AlfalfaGEDB | MS.gene51292

M. sativa cultivar XinJiangDaYe / MS.gene51292

KEGG: -
GO: GO:0003674 GO:0003824 GO:0009055 GO:0016491
PFAM: PF02298.20

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51292.t1	KEH27018.1	74.8	135	32	1	1	133	1	135	1.40E-46	195.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51292.t1	P42849	44.0	100	55	1	27	126	3	101	7.8e-17	88.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51292.t1	A0A072UD60	74.8	135	32	1	1	133	1	135	1.0e-46	195.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049356	MS.gene51292	0.807915	4.00E-50	-1.69E-46
MS.gene049369	MS.gene51292	0.828747	7.57E-55	-1.69E-46
MS.gene050122	MS.gene51292	0.823406	1.41E-53	-1.69E-46
MS.gene050670	MS.gene51292	0.821855	3.23E-53	-1.69E-46
MS.gene05105	MS.gene51292	0.806571	7.71E-50	-1.69E-46
MS.gene052346	MS.gene51292	0.805474	1.31E-49	-1.69E-46
MS.gene052414	MS.gene51292	0.807504	4.89E-50	-1.69E-46
MS.gene053449	MS.gene51292	0.827753	1.31E-54	-1.69E-46
MS.gene053813	MS.gene51292	0.805348	1.39E-49	-1.69E-46
MS.gene05505	MS.gene51292	0.813215	2.86E-51	-1.69E-46
MS.gene055909	MS.gene51292	0.834182	3.48E-56	-1.69E-46
MS.gene055911	MS.gene51292	0.834792	2.44E-56	-1.69E-46
MS.gene056407	MS.gene51292	0.808079	3.69E-50	-1.69E-46
MS.gene056511	MS.gene51292	0.815023	1.14E-51	-1.69E-46
MS.gene05664	MS.gene51292	0.806098	9.70E-50	-1.69E-46
MS.gene05666	MS.gene51292	0.834987	2.18E-56	-1.69E-46
MS.gene057734	MS.gene51292	0.826123	3.22E-54	-1.69E-46
MS.gene057926	MS.gene51292	0.806006	1.01E-49	-1.69E-46
MS.gene058323	MS.gene51292	0.801158	1.03E-48	-1.69E-46
MS.gene058948	MS.gene51292	0.821144	4.72E-53	-1.69E-46
MS.gene060193	MS.gene51292	0.830186	3.38E-55	-1.69E-46
MS.gene060372	MS.gene51292	0.806061	9.88E-50	-1.69E-46
MS.gene060403	MS.gene51292	0.815783	7.74E-52	-1.69E-46
MS.gene060835	MS.gene51292	0.800553	1.37E-48	-1.69E-46
MS.gene060837	MS.gene51292	0.831962	1.24E-55	-1.69E-46
MS.gene061154	MS.gene51292	0.807405	5.14E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51292.t1	MTR_6g083240	81.481	135	23	1	1	133	1	135	2.28e-73	217
MS.gene51292.t1	MTR_4g114870	67.188	128	40	2	1	126	1	128	6.49e-51	166
MS.gene51292.t1	MTR_4g114870	58.416	101	42	0	26	126	190	290	1.25e-38	134
MS.gene51292.t1	MTR_0334s0010	45.833	120	63	2	8	127	9	126	4.49e-30	107
MS.gene51292.t1	MTR_4g067200	39.024	123	73	2	7	127	8	130	3.81e-26	97.1
MS.gene51292.t1	MTR_4g066110	39.024	123	73	2	7	127	8	130	3.81e-26	97.1
MS.gene51292.t1	MTR_3g099580	41.322	121	66	3	6	126	5	120	4.02e-23	89.4
MS.gene51292.t1	MTR_1g104800	35.714	126	77	2	1	126	1	122	1.16e-22	88.2
MS.gene51292.t1	MTR_5g006040	39.048	105	62	1	22	126	21	123	1.51e-22	87.8
MS.gene51292.t1	MTR_3g099570	38.211	123	71	3	6	128	5	122	1.21e-21	85.5
MS.gene51292.t1	MTR_8g089110	38.710	124	67	3	6	127	5	121	2.50e-21	83.2
MS.gene51292.t1	MTR_8g099220	34.167	120	78	1	8	127	10	128	5.04e-21	82.4
MS.gene51292.t1	MTR_8g088830	36.607	112	62	2	16	127	20	122	1.74e-20	80.9
MS.gene51292.t1	MTR_3g099980	42.453	106	56	3	22	126	21	122	6.37e-20	82.0
MS.gene51292.t1	MTR_3g099680	42.453	106	56	3	22	126	21	122	8.61e-20	81.6
MS.gene51292.t1	MTR_1g090420	34.862	109	70	1	18	126	19	126	1.43e-19	81.3
MS.gene51292.t1	MTR_2g043730	41.346	104	54	3	22	125	27	123	2.74e-19	78.2
MS.gene51292.t1	MTR_7g086100	42.056	107	58	2	22	128	21	123	5.54e-19	79.7
MS.gene51292.t1	MTR_7g086280	39.815	108	59	2	22	128	21	123	1.00e-18	77.8
MS.gene51292.t1	MTR_7g086230	39.815	108	59	2	22	128	21	123	1.02e-18	77.8
MS.gene51292.t1	MTR_8g007035	41.905	105	54	3	24	126	21	120	1.19e-18	78.2
MS.gene51292.t1	MTR_8g007020	42.857	105	53	4	24	126	21	120	2.38e-18	77.0
MS.gene51292.t1	MTR_7g086090	40.000	110	62	2	22	131	21	126	1.22e-17	76.3
MS.gene51292.t1	MTR_4g114830	37.113	97	58	3	24	117	25	121	3.14e-17	73.6
MS.gene51292.t1	MTR_3g099540	35.714	126	75	3	3	128	4	123	7.50e-17	72.0
MS.gene51292.t1	MTR_2g088990	40.506	79	46	1	47	125	42	119	7.55e-17	73.9
MS.gene51292.t1	MTR_7g086160	35.246	122	71	3	9	126	6	123	1.24e-16	72.0
MS.gene51292.t1	MTR_6g022170	34.314	102	64	2	26	126	33	132	4.01e-16	70.9
MS.gene51292.t1	MTR_7g086140	35.537	121	71	3	9	126	6	122	9.94e-16	69.7
MS.gene51292.t1	MTR_7g086190	35.537	121	71	3	9	126	6	122	9.94e-16	69.7
MS.gene51292.t1	MTR_3g089035	36.634	101	57	2	24	124	30	123	1.51e-15	68.6
MS.gene51292.t1	MTR_1g105130	34.454	119	71	3	11	126	7	121	1.83e-15	68.9
MS.gene51292.t1	MTR_1g105120	34.454	119	71	3	11	126	7	121	1.83e-15	68.9
MS.gene51292.t1	MTR_2g101300	31.496	127	84	2	1	126	1	125	1.98e-15	69.7
MS.gene51292.t1	MTR_3g089005	32.500	120	74	3	8	127	14	126	5.48e-15	67.0
MS.gene51292.t1	MTR_1g090190	36.538	104	61	2	24	127	20	118	7.01e-15	67.8
MS.gene51292.t1	MTR_1g077790	34.653	101	65	1	27	126	29	129	8.48e-15	68.2
MS.gene51292.t1	MTR_7g086220	33.333	111	68	2	12	120	8	114	1.51e-14	66.6
MS.gene51292.t1	MTR_1g014120	31.193	109	67	2	35	135	34	142	4.28e-14	65.9
MS.gene51292.t1	MTR_2g043790	35.514	107	60	3	22	127	2	100	1.15e-13	62.8
MS.gene51292.t1	MTR_1g112700	28.455	123	83	2	8	129	14	132	3.34e-13	63.5
MS.gene51292.t1	MTR_4g111640	28.099	121	82	3	8	126	14	131	6.50e-13	62.8
MS.gene51292.t1	MTR_8g086360	35.922	103	64	1	24	126	21	121	1.81e-12	61.6
MS.gene51292.t1	MTR_2g090580	31.200	125	72	3	24	137	24	145	3.10e-12	61.2
MS.gene51292.t1	MTR_8g463180	31.624	117	76	2	8	124	7	119	3.77e-12	62.4
MS.gene51292.t1	MTR_2g079030	30.702	114	77	1	9	122	7	118	3.80e-12	62.8
MS.gene51292.t1	MTR_4g081100	28.037	107	77	0	18	124	23	129	3.82e-12	61.2
MS.gene51292.t1	MTR_3g105930	31.034	116	69	2	24	128	24	139	1.81e-11	58.9
MS.gene51292.t1	MTR_4g130780	26.562	128	93	1	1	127	3	130	4.24e-11	58.2
MS.gene51292.t1	MTR_6g023760	34.286	105	63	4	23	126	22	121	5.66e-11	57.8
MS.gene51292.t1	MTR_4g078410	32.039	103	68	1	24	126	22	122	9.52e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51292.t1	AT5G20230	48.039	102	52	1	25	126	23	123	7.27e-29	104
MS.gene51292.t1	AT3G17675	36.364	121	73	1	8	128	13	129	4.20e-23	87.8
MS.gene51292.t1	AT2G32300	39.062	128	73	3	1	128	1	123	9.52e-23	90.5
MS.gene51292.t1	AT2G32300	39.062	128	73	3	1	128	31	153	1.49e-22	90.5
MS.gene51292.t1	AT2G25060	41.525	118	65	3	22	135	26	143	1.64e-20	82.8
MS.gene51292.t1	AT5G26330	35.294	119	75	1	8	126	5	121	2.75e-20	82.4
MS.gene51292.t1	AT3G20570	34.400	125	78	2	1	122	1	124	6.94e-20	81.6
MS.gene51292.t1	AT2G26720	40.164	122	64	5	8	128	14	127	2.34e-19	80.1
MS.gene51292.t1	AT2G31050	38.211	123	72	2	12	133	13	132	4.82e-18	76.6
MS.gene51292.t1	AT3G60270	39.806	103	57	3	24	126	23	120	5.14e-18	76.3
MS.gene51292.t1	AT4G31840	36.364	121	75	2	8	126	7	127	1.12e-17	75.1
MS.gene51292.t1	AT3G60280	38.835	103	58	2	24	126	21	118	4.94e-16	72.0
MS.gene51292.t1	AT4G30590	31.933	119	79	2	8	124	8	126	1.80e-15	69.7
MS.gene51292.t1	AT5G07475	35.577	104	62	2	24	127	27	125	5.03e-15	68.6
MS.gene51292.t1	AT1G72230	40.244	82	48	1	45	126	37	117	6.79e-15	68.2
MS.gene51292.t1	AT2G44790	36.036	111	60	5	18	126	23	124	2.05e-14	67.4
MS.gene51292.t1	AT1G79800	30.645	124	79	3	11	129	14	135	2.36e-14	67.0
MS.gene51292.t1	AT1G22480	31.496	127	74	2	1	127	1	114	3.30e-14	66.2
MS.gene51292.t1	AT1G64640	31.707	123	79	2	8	126	10	131	4.59e-14	66.2
MS.gene51292.t1	AT2G02850	33.621	116	67	3	9	124	21	126	7.52e-14	64.3
MS.gene51292.t1	AT4G27520	33.333	114	74	1	11	124	15	126	1.21e-13	66.6
MS.gene51292.t1	AT3G18590	31.707	123	77	4	8	126	7	126	2.41e-13	64.3
MS.gene51292.t1	AT5G25090	35.052	97	63	0	30	126	30	126	5.51e-13	63.2
MS.gene51292.t1	AT1G45063	33.028	109	70	2	27	134	149	255	5.69e-13	64.3
MS.gene51292.t1	AT1G48940	32.381	105	67	2	27	129	26	128	7.68e-13	62.8
MS.gene51292.t1	AT1G45063	33.028	109	70	2	27	134	149	255	1.43e-12	63.5
MS.gene51292.t1	AT5G14345	26.891	119	81	3	9	126	1	114	1.75e-12	60.8
MS.gene51292.t1	AT4G28365	34.000	100	64	2	27	126	29	126	5.43e-12	60.8
MS.gene51292.t1	AT3G27200	29.752	121	77	4	9	126	6	121	7.97e-12	59.7
MS.gene51292.t1	AT4G32490	26.891	119	85	1	8	126	12	128	4.39e-11	58.5
MS.gene51292.t1	AT4G01380	34.694	98	61	3	34	129	79	175	7.40e-11	57.8
MS.gene51292.t1	AT3G53330	34.906	106	66	2	24	128	185	288	7.49e-11	58.5

Find 33 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATTTCATATGTACCGTTAC+TGG	0.314883	6.3:-78038718	MS.gene51292:CDS
ACCATGGCAAGCAACATAAA+TGG	0.379823	6.3:-78039124	MS.gene51292:CDS
CTATATCAGTGGAAGGGTGC+TGG	0.436825	6.3:+78038640	None:intergenic
CTCATGAGAGACACCTTAGC+TGG	0.440760	6.3:+78038754	None:intergenic
GATCACAATTCTATGTCAAC+TGG	0.459472	6.3:-78039000	MS.gene51292:CDS
GCAGTGGCTAACCTTCTTCA+TGG	0.479052	6.3:-78039082	MS.gene51292:CDS
ACCATTTATGTTGCTTGCCA+TGG	0.484297	6.3:+78039123	None:intergenic
AAGGTGTCTCTCATGAGTGC+AGG	0.497615	6.3:-78038748	MS.gene51292:CDS
CCGTGGGTGATGACTTAGGC+TGG	0.505012	6.3:-78039039	MS.gene51292:CDS
GGCTGGATTATCCCAACCGT+TGG	0.506002	6.3:-78039022	MS.gene51292:CDS
GTTACTGGTCACTGTTCTGC+TGG	0.511691	6.3:-78038703	MS.gene51292:CDS
TAGAATTGTGATCCAACGGT+TGG	0.521337	6.3:+78039010	None:intergenic
TTGCTACGGATCCATGAAGA+AGG	0.528261	6.3:+78039071	None:intergenic
GAATGCACACGCCGTGGCAA+AGG	0.533207	6.3:-78038839	MS.gene51292:CDS
AGAATTGTGATCCAACGGTT+GGG	0.536039	6.3:+78039011	None:intergenic
TCTGCTTTCGATAGTTGCAA+TGG	0.540088	6.3:-78038808	MS.gene51292:CDS
CTTAAATCTATATCAGTGGA+AGG	0.546850	6.3:+78038633	None:intergenic
CAATGGAACCAACGCACTTG+TGG	0.564659	6.3:-78038791	MS.gene51292:CDS
TAAGTCATCACCCACGGTGA+AGG	0.570272	6.3:+78039045	None:intergenic
TATGACAAAGCCTCCAGCTA+AGG	0.573588	6.3:-78038767	MS.gene51292:CDS
GACATAGAATTGTGATCCAA+CGG	0.579776	6.3:+78039006	None:intergenic
AGCAGAACAGTGACCAGTAA+CGG	0.582060	6.3:+78038705	None:intergenic
TGTCATAACCACAAGTGCGT+TGG	0.590117	6.3:+78038783	None:intergenic
AGGTGTCTCTCATGAGTGCA+GGG	0.602357	6.3:-78038747	MS.gene51292:CDS
TTCACCGTAGGTGACACTCT+TGG	0.604216	6.3:-78038962	MS.gene51292:intron
TTCACCGTGGGTGATGACTT+AGG	0.609147	6.3:-78039043	MS.gene51292:CDS
TTAAATCTATATCAGTGGAA+GGG	0.611961	6.3:+78038634	None:intergenic
TCAATTGAATGCACACGCCG+TGG	0.614375	6.3:-78038845	MS.gene51292:CDS
AAGAGTGTCACCTACGGTGA+AGG	0.615747	6.3:+78038964	None:intergenic
ACCAATAAAACCTTCACCGT+AGG	0.655037	6.3:-78038974	MS.gene51292:CDS
ATGAGAGACACCTTAGCTGG+AGG	0.666881	6.3:+78038757	None:intergenic
CCAGCCTAAGTCATCACCCA+CGG	0.672113	6.3:+78039039	None:intergenic
CATACCAAGAGTGTCACCTA+CGG	0.673615	6.3:+78038958	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TATTTCATATGTACCGTTAC+TGG	-	chr6.3:78039048-78039067	MS.gene51292:CDS	30.0%
	GACATAGAATTGTGATCCAA+CGG	+	chr6.3:78038763-78038782	None:intergenic	35.0%
	GATCACAATTCTATGTCAAC+TGG	-	chr6.3:78038766-78038785	MS.gene51292:CDS	35.0%
!	AGATTTTGTTACCTTTGCCA+CGG	+	chr6.3:78038941-78038960	None:intergenic	35.0%
!	GGTGCTGGAAAAAAGATAAT+CGG	+	chr6.3:78039114-78039133	None:intergenic	35.0%
	ACCAATAAAACCTTCACCGT+AGG	-	chr6.3:78038792-78038811	MS.gene51292:CDS	40.0%
	AGAATTGTGATCCAACGGTT+GGG	+	chr6.3:78038758-78038777	None:intergenic	40.0%
	TAGAATTGTGATCCAACGGT+TGG	+	chr6.3:78038759-78038778	None:intergenic	40.0%
	TCTGCTTTCGATAGTTGCAA+TGG	-	chr6.3:78038958-78038977	MS.gene51292:intron	40.0%
!!	ACCTACGGTGAAGGTTTTAT+TGG	+	chr6.3:78038796-78038815	None:intergenic	40.0%
	AGCAGAACAGTGACCAGTAA+CGG	+	chr6.3:78039064-78039083	None:intergenic	45.0%
	GTAGCAAAAACCTTCACCGT+GGG	-	chr6.3:78038711-78038730	MS.gene51292:CDS	45.0%
	TATGACAAAGCCTCCAGCTA+AGG	-	chr6.3:78038999-78039018	MS.gene51292:CDS	45.0%
	TGTCATAACCACAAGTGCGT+TGG	+	chr6.3:78038986-78039005	None:intergenic	45.0%
	TTGCTACGGATCCATGAAGA+AGG	+	chr6.3:78038698-78038717	None:intergenic	45.0%
!	CACGGTGAAGGTTTTTGCTA+CGG	+	chr6.3:78038712-78038731	None:intergenic	45.0%
!!	CATACCAAGAGTGTCACCTA+CGG	+	chr6.3:78038811-78038830	None:intergenic	45.0%
	AGGTGTCTCTCATGAGTGCA+GGG	-	chr6.3:78039019-78039038	MS.gene51292:CDS	50.0%
	ATGAGAGACACCTTAGCTGG+AGG	+	chr6.3:78039012-78039031	None:intergenic	50.0%
	CAATGGAACCAACGCACTTG+TGG	-	chr6.3:78038975-78038994	MS.gene51292:CDS	50.0%
	CGTAGCAAAAACCTTCACCG+TGG	-	chr6.3:78038710-78038729	MS.gene51292:CDS	50.0%
	CTCATGAGAGACACCTTAGC+TGG	+	chr6.3:78039015-78039034	None:intergenic	50.0%
	GCAGTGGCTAACCTTCTTCA+TGG	-	chr6.3:78038684-78038703	MS.gene51292:CDS	50.0%
	GTTACTGGTCACTGTTCTGC+TGG	-	chr6.3:78039063-78039082	MS.gene51292:CDS	50.0%
	TAAGTCATCACCCACGGTGA+AGG	+	chr6.3:78038724-78038743	None:intergenic	50.0%
	TCAATTGAATGCACACGCCG+TGG	-	chr6.3:78038921-78038940	MS.gene51292:intron	50.0%
	TTCACCGTAGGTGACACTCT+TGG	-	chr6.3:78038804-78038823	MS.gene51292:CDS	50.0%
	TTCACCGTGGGTGATGACTT+AGG	-	chr6.3:78038723-78038742	MS.gene51292:CDS	50.0%
!	AAGGTGTCTCTCATGAGTGC+AGG	-	chr6.3:78039018-78039037	MS.gene51292:CDS	50.0%
!!	AAGAGTGTCACCTACGGTGA+AGG	+	chr6.3:78038805-78038824	None:intergenic	50.0%
!!	TGTTTTTGTGCTGCTCGCAG+TGG	-	chr6.3:78038668-78038687	MS.gene51292:CDS	50.0%
	CCAGCCTAAGTCATCACCCA+CGG	+	chr6.3:78038730-78038749	None:intergenic	55.0%
	GGCTGGATTATCCCAACCGT+TGG	-	chr6.3:78038744-78038763	MS.gene51292:CDS	55.0%
	CCGTGGGTGATGACTTAGGC+TGG	-	chr6.3:78038727-78038746	MS.gene51292:CDS	60.0%
	GAATGCACACGCCGTGGCAA+AGG	-	chr6.3:78038927-78038946	MS.gene51292:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	78038645	78039143	78038645	ID=MS.gene51292
chr6.3	mRNA	78038645	78039143	78038645	ID=MS.gene51292.t1;Parent=MS.gene51292
chr6.3	exon	78038963	78039143	78038963	ID=MS.gene51292.t1.exon1;Parent=MS.gene51292.t1
chr6.3	CDS	78038963	78039143	78038963	ID=cds.MS.gene51292.t1;Parent=MS.gene51292.t1
chr6.3	exon	78038645	78038877	78038645	ID=MS.gene51292.t1.exon2;Parent=MS.gene51292.t1
chr6.3	CDS	78038645	78038877	78038645	ID=cds.MS.gene51292.t1;Parent=MS.gene51292.t1

Gene Sequence

>MS.gene51292

ATGGCAAGCAACATAAATGGTCTTGTTTTTGTGCTGCTCGCAGTGGCTAACCTTCTTCATGGATCCGTAGCAAAAACCTTCACCGTGGGTGATGACTTAGGCTGGATTATCCCAACCGTTGGATCACAATTCTATGTCAACTGGTCGACCAATAAAACCTTCACCGTAGGTGACACTCTTGGTATGTAAGATATCATGTAAATCTTAGCTATTAATATTAATAACTATAAATCAGATTATTTTAATTAATTTCTTTTAGCAAACAGTGTTCAACTTTCAATTGAATGCACACGCCGTGGCAAAGGTAACAAAATCTGCTTTCGATAGTTGCAATGGAACCAACGCACTTGTGGTTATGACAAAGCCTCCAGCTAAGGTGTCTCTCATGAGTGCAGGGCAACAATATTTCATATGTACCGTTACTGGTCACTGTTCTGCTGGTCAGAAACTGTCGATTAACGTTTCTCCGATTATCTTTTTTCCAGCACCCTTCCACTGA

Protein sequence

>MS.gene51292.t1

MASNINGLVFVLLAVANLLHGSVAKTFTVGDDLGWIIPTVGSQFYVNWSTNKTFTVGDTLVFNFQLNAHAVAKVTKSAFDSCNGTNALVVMTKPPAKVSLMSAGQQYFICTVTGHCSAGQKLSINVSPIIFFPAPFH