Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56293.t1 | XP_003598314.2 | 94.2 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 1.20E-69 | 272.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56293.t1 | A6QLK2 | 36.1 | 133 | 79 | 3 | 4 | 135 | 7 | 134 | 9.5e-15 | 81.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56293.t1 | G7J0F9 | 94.2 | 137 | 8 | 0 | 1 | 137 | 60 | 196 | 8.7e-70 | 272.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049821 | MS.gene56293 | 0.810607 | 1.06E-50 | -1.69E-46 |
| MS.gene050237 | MS.gene56293 | 0.824687 | 7.05E-54 | -1.69E-46 |
| MS.gene050303 | MS.gene56293 | 0.810638 | 1.04E-50 | -1.69E-46 |
| MS.gene050719 | MS.gene56293 | 0.812489 | 4.13E-51 | -1.69E-46 |
| MS.gene050721 | MS.gene56293 | 0.813741 | 2.20E-51 | -1.69E-46 |
| MS.gene050750 | MS.gene56293 | 0.821231 | 4.51E-53 | -1.69E-46 |
| MS.gene050953 | MS.gene56293 | 0.82026 | 7.53E-53 | -1.69E-46 |
| MS.gene05134 | MS.gene56293 | 0.816622 | 5.03E-52 | -1.69E-46 |
| MS.gene052176 | MS.gene56293 | 0.803904 | 2.79E-49 | -1.69E-46 |
| MS.gene052358 | MS.gene56293 | -0.812577 | 3.95E-51 | -1.69E-46 |
| MS.gene052376 | MS.gene56293 | 0.806621 | 7.53E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene56293 | 0.830799 | 2.40E-55 | -1.69E-46 |
| MS.gene052863 | MS.gene56293 | 0.810417 | 1.16E-50 | -1.69E-46 |
| MS.gene053006 | MS.gene56293 | 0.843848 | 1.09E-58 | -1.69E-46 |
| MS.gene053008 | MS.gene56293 | 0.801455 | 8.93E-49 | -1.69E-46 |
| MS.gene05415 | MS.gene56293 | 0.827884 | 1.22E-54 | -1.69E-46 |
| MS.gene054405 | MS.gene56293 | 0.814239 | 1.70E-51 | -1.69E-46 |
| MS.gene054472 | MS.gene56293 | 0.810005 | 1.43E-50 | -1.69E-46 |
| MS.gene055623 | MS.gene56293 | 0.809527 | 1.81E-50 | -1.69E-46 |
| MS.gene055818 | MS.gene56293 | 0.834092 | 3.66E-56 | -1.69E-46 |
| MS.gene056498 | MS.gene56293 | 0.800537 | 1.38E-48 | -1.69E-46 |
| MS.gene057060 | MS.gene56293 | 0.805221 | 1.48E-49 | -1.69E-46 |
| MS.gene057079 | MS.gene56293 | 0.810734 | 9.95E-51 | -1.69E-46 |
| MS.gene057414 | MS.gene56293 | 0.814219 | 1.72E-51 | -1.69E-46 |
| MS.gene05795 | MS.gene56293 | 0.801344 | 9.41E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene56293 | 0.804619 | 1.98E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene56293 | 0.819751 | 9.85E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene56293 | 0.817253 | 3.63E-52 | -1.69E-46 |
| MS.gene060429 | MS.gene56293 | 0.83412 | 3.60E-56 | -1.69E-46 |
| MS.gene060521 | MS.gene56293 | 0.812265 | 4.63E-51 | -1.69E-46 |
| MS.gene060572 | MS.gene56293 | 0.827798 | 1.28E-54 | -1.69E-46 |
| MS.gene060634 | MS.gene56293 | -0.807218 | 5.63E-50 | -1.69E-46 |
| MS.gene060917 | MS.gene56293 | 0.806467 | 8.11E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56293.t1 | MTR_3g010210 | 94.161 | 137 | 8 | 0 | 1 | 137 | 1 | 137 | 2.58e-97 | 276 |
| MS.gene56293.t1 | MTR_3g010210 | 94.161 | 137 | 8 | 0 | 1 | 137 | 60 | 196 | 1.52e-96 | 276 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56293.t1 | AT5G63690 | 66.176 | 136 | 45 | 1 | 2 | 136 | 3 | 138 | 2.42e-66 | 197 |
| MS.gene56293.t1 | AT5G63690 | 66.176 | 136 | 45 | 1 | 2 | 136 | 3 | 138 | 2.42e-66 | 197 |
Find 31 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACACTAAATTTATAATTT+TGG | 0.156979 | 3.1:-5257543 | MS.gene56293:CDS |
| TGCAATTCATTTACAACTTT+GGG | 0.191576 | 3.1:-5257453 | MS.gene56293:CDS |
| CACATCAAAATCTACAAAAT+TGG | 0.250053 | 3.1:+5257185 | None:intergenic |
| CTGCAATTCATTTACAACTT+TGG | 0.295983 | 3.1:-5257454 | MS.gene56293:CDS |
| TCTTACCTGCCCTTAGTATA+AGG | 0.314556 | 3.1:+5257354 | None:intergenic |
| TTCTTAGAATTTGTTGGGTC+AGG | 0.345198 | 3.1:+5257254 | None:intergenic |
| TGGAGAGAAGGCACGGGAAT+GGG | 0.347886 | 3.1:+5257205 | None:intergenic |
| CAAACATGAGTGAGATTCAT+TGG | 0.355934 | 3.1:-5257280 | MS.gene56293:CDS |
| TGCGTGGCAACCTTATACTA+AGG | 0.364864 | 3.1:-5257364 | MS.gene56293:CDS |
| AGAGGAAAGCTTGAGAAGAT+AGG | 0.378036 | 3.1:-5257332 | MS.gene56293:CDS |
| TATACTTCTTAGAATTTGTT+GGG | 0.390203 | 3.1:+5257249 | None:intergenic |
| CAATGAATCTCACTCATGTT+TGG | 0.429356 | 3.1:+5257281 | None:intergenic |
| ATATACTTCTTAGAATTTGT+TGG | 0.431038 | 3.1:+5257248 | None:intergenic |
| GGGGAGATATGACATACTCT+TGG | 0.503412 | 3.1:+5257225 | None:intergenic |
| TTGGAGAGAAGGCACGGGAA+TGG | 0.503623 | 3.1:+5257204 | None:intergenic |
| AAAGGGAGGATAACACTTGA+AGG | 0.510258 | 3.1:-5257518 | MS.gene56293:CDS |
| GGAGAGAAGGCACGGGAATG+GGG | 0.529940 | 3.1:+5257206 | None:intergenic |
| AATCTACAAAATTGGAGAGA+AGG | 0.536686 | 3.1:+5257193 | None:intergenic |
| CATGTTTGGTGTCTCAACAT+AGG | 0.565946 | 3.1:+5257295 | None:intergenic |
| ACAAAATTGGAGAGAAGGCA+CGG | 0.567717 | 3.1:+5257198 | None:intergenic |
| TATAAGGTTGCCACGCAGGT+AGG | 0.568146 | 3.1:+5257370 | None:intergenic |
| GGCAACCTTATACTAAGGGC+AGG | 0.570742 | 3.1:-5257359 | MS.gene56293:CDS |
| GCAATTCATTTACAACTTTG+GGG | 0.580709 | 3.1:-5257452 | MS.gene56293:CDS |
| TTAGTATAAGGTTGCCACGC+AGG | 0.586370 | 3.1:+5257366 | None:intergenic |
| ATACTAAGGGCAGGTAAGAG+AGG | 0.591856 | 3.1:-5257350 | MS.gene56293:CDS |
| CAGGTAGGAAAAGATACCAT+TGG | 0.607789 | 3.1:+5257385 | None:intergenic |
| CAAAATTGGAGAGAAGGCAC+GGG | 0.629659 | 3.1:+5257199 | None:intergenic |
| GACATCGTTTATATGACCAA+TGG | 0.633538 | 3.1:-5257401 | MS.gene56293:CDS |
| AAGGGAGGATAACACTTGAA+GGG | 0.653494 | 3.1:-5257517 | MS.gene56293:CDS |
| GCGTGGCAACCTTATACTAA+GGG | 0.670757 | 3.1:-5257363 | MS.gene56293:CDS |
| AAGTTGTAAATGAATTGCAG+CGG | 0.683257 | 3.1:+5257457 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTATAATTTTGGAGAA+AGG | - | chr3.1:5257247-5257266 | MS.gene56293:CDS | 15.0% |
| !!! | AATTTATAATTTTGGAGAAA+GGG | - | chr3.1:5257248-5257267 | MS.gene56293:CDS | 15.0% |
| !!! | AGACACTAAATTTATAATTT+TGG | - | chr3.1:5257240-5257259 | MS.gene56293:CDS | 15.0% |
| !! | ATATACTTCTTAGAATTTGT+TGG | + | chr3.1:5257538-5257557 | None:intergenic | 20.0% |
| !!! | TATACTTCTTAGAATTTGTT+GGG | + | chr3.1:5257537-5257556 | None:intergenic | 20.0% |
| ! | TGCAATTCATTTACAACTTT+GGG | - | chr3.1:5257330-5257349 | MS.gene56293:CDS | 25.0% |
| !!! | GAATGTGATTATTTTGATTC+AGG | - | chr3.1:5257358-5257377 | MS.gene56293:CDS | 25.0% |
| AAGTTGTAAATGAATTGCAG+CGG | + | chr3.1:5257329-5257348 | None:intergenic | 30.0% | |
| CTGCAATTCATTTACAACTT+TGG | - | chr3.1:5257329-5257348 | MS.gene56293:CDS | 30.0% | |
| GCAATTCATTTACAACTTTG+GGG | - | chr3.1:5257331-5257350 | MS.gene56293:CDS | 30.0% | |
| !! | AGGGCAAAATAAAAAGTGTT+TGG | - | chr3.1:5257285-5257304 | MS.gene56293:CDS | 30.0% |
| !! | TTATAATTTTGGAGAAAGGG+AGG | - | chr3.1:5257251-5257270 | MS.gene56293:CDS | 30.0% |
| CAAACATGAGTGAGATTCAT+TGG | - | chr3.1:5257503-5257522 | MS.gene56293:CDS | 35.0% | |
| CAATGAATCTCACTCATGTT+TGG | + | chr3.1:5257505-5257524 | None:intergenic | 35.0% | |
| GACATCGTTTATATGACCAA+TGG | - | chr3.1:5257382-5257401 | MS.gene56293:CDS | 35.0% | |
| !! | TTCTTAGAATTTGTTGGGTC+AGG | + | chr3.1:5257532-5257551 | None:intergenic | 35.0% |
| AAAGGGAGGATAACACTTGA+AGG | - | chr3.1:5257265-5257284 | MS.gene56293:CDS | 40.0% | |
| AAGGGAGGATAACACTTGAA+GGG | - | chr3.1:5257266-5257285 | MS.gene56293:CDS | 40.0% | |
| ACAAAATTGGAGAGAAGGCA+CGG | + | chr3.1:5257588-5257607 | None:intergenic | 40.0% | |
| AGAGGAAAGCTTGAGAAGAT+AGG | - | chr3.1:5257451-5257470 | MS.gene56293:CDS | 40.0% | |
| CAGGTAGGAAAAGATACCAT+TGG | + | chr3.1:5257401-5257420 | None:intergenic | 40.0% | |
| TCTTACCTGCCCTTAGTATA+AGG | + | chr3.1:5257432-5257451 | None:intergenic | 40.0% | |
| !! | CATGTTTGGTGTCTCAACAT+AGG | + | chr3.1:5257491-5257510 | None:intergenic | 40.0% |
| !!! | TCTATGTTGTTTTGAGCTGC+AGG | + | chr3.1:5257223-5257242 | None:intergenic | 40.0% |
| ATACTAAGGGCAGGTAAGAG+AGG | - | chr3.1:5257433-5257452 | MS.gene56293:CDS | 45.0% | |
| GCGTGGCAACCTTATACTAA+GGG | - | chr3.1:5257420-5257439 | MS.gene56293:CDS | 45.0% | |
| GGGGAGATATGACATACTCT+TGG | + | chr3.1:5257561-5257580 | None:intergenic | 45.0% | |
| TGCGTGGCAACCTTATACTA+AGG | - | chr3.1:5257419-5257438 | MS.gene56293:CDS | 45.0% | |
| TTAGTATAAGGTTGCCACGC+AGG | + | chr3.1:5257420-5257439 | None:intergenic | 45.0% | |
| ! | CAAAATTGGAGAGAAGGCAC+GGG | + | chr3.1:5257587-5257606 | None:intergenic | 45.0% |
| GGCAACCTTATACTAAGGGC+AGG | - | chr3.1:5257424-5257443 | MS.gene56293:CDS | 50.0% | |
| TATAAGGTTGCCACGCAGGT+AGG | + | chr3.1:5257416-5257435 | None:intergenic | 50.0% | |
| ! | GGTATCTTTTCCTACCTGCG+TGG | - | chr3.1:5257403-5257422 | MS.gene56293:CDS | 50.0% |
| ! | TGGAGAGAAGGCACGGGAAT+GGG | + | chr3.1:5257581-5257600 | None:intergenic | 55.0% |
| ! | TTGGAGAGAAGGCACGGGAA+TGG | + | chr3.1:5257582-5257601 | None:intergenic | 55.0% |
| ! | GGAGAGAAGGCACGGGAATG+GGG | + | chr3.1:5257580-5257599 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 5257196 | 5257609 | 5257196 | ID=MS.gene56293 |
| chr3.1 | mRNA | 5257196 | 5257609 | 5257196 | ID=MS.gene56293.t1;Parent=MS.gene56293 |
| chr3.1 | exon | 5257196 | 5257609 | 5257196 | ID=MS.gene56293.t1.exon1;Parent=MS.gene56293.t1 |
| chr3.1 | CDS | 5257196 | 5257609 | 5257196 | ID=cds.MS.gene56293.t1;Parent=MS.gene56293.t1 |
| Gene Sequence |
| Protein sequence |
