Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58141.t1 | XP_003630148.1 | 93.9 | 82 | 5 | 0 | 1 | 82 | 1 | 82 | 8.10E-29 | 136 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58141.t1 | G7LC65 | 93.9 | 82 | 5 | 0 | 1 | 82 | 1 | 82 | 5.8e-29 | 136.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene58141 | 0.863109 | 3.15E-64 | -1.69E-46 |
| MS.gene049993 | MS.gene58141 | 0.803471 | 3.43E-49 | -1.69E-46 |
| MS.gene050406 | MS.gene58141 | 0.812674 | 3.76E-51 | -1.69E-46 |
| MS.gene050426 | MS.gene58141 | -0.801866 | 7.35E-49 | -1.69E-46 |
| MS.gene051208 | MS.gene58141 | 0.801912 | 7.20E-49 | -1.69E-46 |
| MS.gene051525 | MS.gene58141 | 0.81208 | 5.08E-51 | -1.69E-46 |
| MS.gene051582 | MS.gene58141 | 0.823001 | 1.75E-53 | -1.69E-46 |
| MS.gene052011 | MS.gene58141 | -0.835429 | 1.69E-56 | -1.69E-46 |
| MS.gene052134 | MS.gene58141 | 0.83079 | 2.41E-55 | -1.69E-46 |
| MS.gene052135 | MS.gene58141 | 0.841746 | 3.94E-58 | -1.69E-46 |
| MS.gene052253 | MS.gene58141 | 0.82518 | 5.39E-54 | -1.69E-46 |
| MS.gene052481 | MS.gene58141 | 0.81122 | 7.81E-51 | -1.69E-46 |
| MS.gene052589 | MS.gene58141 | 0.80738 | 5.20E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene58141 | 0.844722 | 6.34E-59 | -1.69E-46 |
| MS.gene054102 | MS.gene58141 | 0.807659 | 4.54E-50 | -1.69E-46 |
| MS.gene05413 | MS.gene58141 | 0.804052 | 2.60E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene58141 | 0.807352 | 5.27E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene58141 | 0.818207 | 2.21E-52 | -1.69E-46 |
| MS.gene05553 | MS.gene58141 | 0.808507 | 2.99E-50 | -1.69E-46 |
| MS.gene056633 | MS.gene58141 | 0.819023 | 1.44E-52 | -1.69E-46 |
| MS.gene05702 | MS.gene58141 | 0.808155 | 3.56E-50 | -1.69E-46 |
| MS.gene058746 | MS.gene58141 | 0.825171 | 5.42E-54 | -1.69E-46 |
| MS.gene059011 | MS.gene58141 | 0.83837 | 2.99E-57 | -1.69E-46 |
| MS.gene059420 | MS.gene58141 | 0.833274 | 5.86E-56 | -1.69E-46 |
| MS.gene060403 | MS.gene58141 | 0.808648 | 2.79E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene58141 | 0.819705 | 1.01E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene58141.t1 | MTR_8g092320 | 93.902 | 82 | 5 | 0 | 1 | 82 | 1 | 82 | 2.54e-44 | 137 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 14 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACTAGGAGACTTTAATTA+TGG | 0.215662 | 4.2:+9875760 | None:intergenic |
| CTTGTGTTTGTGTCCCTTCA+TGG | 0.225941 | 4.2:+9875862 | None:intergenic |
| TACTAAAGATGGAGCTGAAA+AGG | 0.386508 | 4.2:-9875910 | MS.gene58141:CDS |
| AGGAGACTTTAATTATGGAT+TGG | 0.479268 | 4.2:+9875765 | None:intergenic |
| AGACTTTAATTATGGATTGG+TGG | 0.484159 | 4.2:+9875768 | None:intergenic |
| ATGGATTGGTGGATTCTTTG+AGG | 0.484420 | 4.2:+9875779 | None:intergenic |
| GCAACAAGAAGGCGTTTGCT+TGG | 0.499691 | 4.2:+9876210 | None:intergenic |
| ATTGGTGGATTCTTTGAGGC+AGG | 0.510445 | 4.2:+9875783 | None:intergenic |
| ACTAAAGATGGAGCTGAAAA+GGG | 0.528078 | 4.2:-9875909 | MS.gene58141:CDS |
| TTGTTTAAGCTAAACCATGA+AGG | 0.545024 | 4.2:-9875876 | MS.gene58141:CDS |
| TGATGACTTGTTGGATACAA+TGG | 0.554869 | 4.2:-9875814 | MS.gene58141:CDS |
| AACTTGAGAGATACTAAAGA+TGG | 0.564007 | 4.2:-9875921 | MS.gene58141:CDS |
| TTCATCTTAACTACAGCTCA+AGG | 0.570896 | 4.2:-9876171 | MS.gene58141:intron |
| TGTTTAAGCTAAACCATGAA+GGG | 0.579354 | 4.2:-9875875 | MS.gene58141:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAATATTTGTATATTTG+AGG | - | chr4.2:9876007-9876026 | MS.gene58141:intron | 10.0% |
| !! | GAAAACATCACATAAATTAT+AGG | + | chr4.2:9876033-9876052 | None:intergenic | 20.0% |
| !!! | ATTTATGTGATGTTTTCTTT+TGG | - | chr4.2:9876036-9876055 | MS.gene58141:intron | 20.0% |
| !! | AGAAAAAAATGATGACTTGT+TGG | - | chr4.2:9876172-9876191 | MS.gene58141:CDS | 25.0% |
| !! | TGCATCGTTCATTAATTTTA+AGG | - | chr4.2:9875887-9875906 | MS.gene58141:CDS | 25.0% |
| AACTTGAGAGATACTAAAGA+TGG | - | chr4.2:9876074-9876093 | MS.gene58141:intron | 30.0% | |
| TCAACAAAAATGCAACAAGA+AGG | + | chr4.2:9875799-9875818 | None:intergenic | 30.0% | |
| TGTTTAAGCTAAACCATGAA+GGG | - | chr4.2:9876120-9876139 | MS.gene58141:intron | 30.0% | |
| TTGTTTAAGCTAAACCATGA+AGG | - | chr4.2:9876119-9876138 | MS.gene58141:intron | 30.0% | |
| ACTAAAGATGGAGCTGAAAA+GGG | - | chr4.2:9876086-9876105 | MS.gene58141:intron | 35.0% | |
| TACTAAAGATGGAGCTGAAA+AGG | - | chr4.2:9876085-9876104 | MS.gene58141:intron | 35.0% | |
| TTCATCTTAACTACAGCTCA+AGG | - | chr4.2:9875824-9875843 | MS.gene58141:CDS | 35.0% | |
| !! | TGATGACTTGTTGGATACAA+TGG | - | chr4.2:9876181-9876200 | MS.gene58141:CDS | 35.0% |
| ATTAATGAACGATGCAGCAG+TGG | + | chr4.2:9875883-9875902 | None:intergenic | 40.0% | |
| !! | ATGGATTGGTGGATTCTTTG+AGG | + | chr4.2:9876219-9876238 | None:intergenic | 40.0% |
| CTTGTGTTTGTGTCCCTTCA+TGG | + | chr4.2:9876136-9876155 | None:intergenic | 45.0% | |
| !! | ATTGGTGGATTCTTTGAGGC+AGG | + | chr4.2:9876215-9876234 | None:intergenic | 45.0% |
| ATGAACGATGCAGCAGTGGT+AGG | + | chr4.2:9875879-9875898 | None:intergenic | 50.0% | |
| !! | GCAACAAGAAGGCGTTTGCT+TGG | + | chr4.2:9875788-9875807 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 9875773 | 9876244 | 9875773 | ID=MS.gene58141 |
| chr4.2 | mRNA | 9875773 | 9876244 | 9875773 | ID=MS.gene58141.t1;Parent=MS.gene58141 |
| chr4.2 | exon | 9876172 | 9876244 | 9876172 | ID=MS.gene58141.t1.exon1;Parent=MS.gene58141.t1 |
| chr4.2 | CDS | 9876172 | 9876244 | 9876172 | ID=cds.MS.gene58141.t1;Parent=MS.gene58141.t1 |
| chr4.2 | exon | 9875773 | 9875948 | 9875773 | ID=MS.gene58141.t1.exon2;Parent=MS.gene58141.t1 |
| chr4.2 | CDS | 9875773 | 9875948 | 9875773 | ID=cds.MS.gene58141.t1;Parent=MS.gene58141.t1 |
| Gene Sequence |
| Protein sequence |
