Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59002.t1 | XP_013445956.1 | 91.5 | 130 | 11 | 0 | 27 | 156 | 36 | 165 | 6.60E-64 | 253.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59002.t1 | Q39224 | 52.6 | 116 | 54 | 1 | 42 | 156 | 52 | 167 | 5.2e-33 | 142.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59002.t1 | A0A072TR49 | 91.5 | 130 | 11 | 0 | 27 | 156 | 41 | 170 | 4.8e-64 | 253.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene59002 | 0.815312 | 9.86E-52 | -1.69E-46 |
| MS.gene050647 | MS.gene59002 | 0.805239 | 1.47E-49 | -1.69E-46 |
| MS.gene051138 | MS.gene59002 | 0.800223 | 1.59E-48 | -1.69E-46 |
| MS.gene051385 | MS.gene59002 | 0.808192 | 3.49E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene59002 | 0.816499 | 5.36E-52 | -1.69E-46 |
| MS.gene05240 | MS.gene59002 | 0.817245 | 3.64E-52 | -1.69E-46 |
| MS.gene052606 | MS.gene59002 | 0.8105 | 1.12E-50 | -1.69E-46 |
| MS.gene053031 | MS.gene59002 | 0.809287 | 2.04E-50 | -1.69E-46 |
| MS.gene053033 | MS.gene59002 | 0.815081 | 1.11E-51 | -1.69E-46 |
| MS.gene053403 | MS.gene59002 | 0.810787 | 9.69E-51 | -1.69E-46 |
| MS.gene053406 | MS.gene59002 | 0.840884 | 6.65E-58 | -1.69E-46 |
| MS.gene054146 | MS.gene59002 | 0.803362 | 3.61E-49 | -1.69E-46 |
| MS.gene054742 | MS.gene59002 | 0.808346 | 3.24E-50 | -1.69E-46 |
| MS.gene055124 | MS.gene59002 | 0.80159 | 8.38E-49 | -1.69E-46 |
| MS.gene055202 | MS.gene59002 | 0.803381 | 3.58E-49 | -1.69E-46 |
| MS.gene055240 | MS.gene59002 | 0.816247 | 6.10E-52 | -1.69E-46 |
| MS.gene056218 | MS.gene59002 | 0.834317 | 3.21E-56 | -1.69E-46 |
| MS.gene056590 | MS.gene59002 | 0.804484 | 2.11E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene59002 | 0.805332 | 1.41E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene59002 | 0.817934 | 2.55E-52 | -1.69E-46 |
| MS.gene057544 | MS.gene59002 | 0.832809 | 7.65E-56 | -1.69E-46 |
| MS.gene057550 | MS.gene59002 | 0.801622 | 8.25E-49 | -1.69E-46 |
| MS.gene058171 | MS.gene59002 | 0.802274 | 6.06E-49 | -1.69E-46 |
| MS.gene059037 | MS.gene59002 | 0.800491 | 1.41E-48 | -1.69E-46 |
| MS.gene059045 | MS.gene59002 | 0.808788 | 2.61E-50 | -1.69E-46 |
| MS.gene060593 | MS.gene59002 | 0.802532 | 5.36E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 38 sgRNAs with CRISPR-Local
Find 77 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGAGAATGGGGTTTCTTTC+AGG | 0.083963 | 5.1:+37497902 | MS.gene59002:CDS |
| TCACGGAGTCAATCCTTTAT+TGG | 0.222974 | 5.1:+37498811 | MS.gene59002:CDS |
| CTAGTATTCCCCAACAATTC+AGG | 0.279797 | 5.1:+37499029 | MS.gene59002:CDS |
| GGAATACTAGGAAATAAGTT+TGG | 0.339541 | 5.1:-37499016 | None:intergenic |
| ATCTCAGAAACCAGGTGATA+TGG | 0.344452 | 5.1:+37498898 | MS.gene59002:CDS |
| CTGAATTGTTGGGGAATACT+AGG | 0.351495 | 5.1:-37499028 | None:intergenic |
| CCAGGTGATATGGAAGGTTT+TGG | 0.352253 | 5.1:+37498908 | MS.gene59002:CDS |
| TCTCTTGGTGCCTTCTGTCC+AGG | 0.394890 | 5.1:+37497711 | MS.gene59002:CDS |
| GGTATCAGAGGATCACAAAT+TGG | 0.402340 | 5.1:+37498943 | MS.gene59002:CDS |
| TGATCATGCCTGCAGAGAAT+GGG | 0.418939 | 5.1:+37497890 | MS.gene59002:CDS |
| TTGATCATGCCTGCAGAGAA+TGG | 0.426303 | 5.1:+37497889 | MS.gene59002:CDS |
| GGGAAAGAACCTGAATTGTT+GGG | 0.431712 | 5.1:-37499038 | None:intergenic |
| TGGGTTGCTTTACTATCTCC+TGG | 0.435852 | 5.1:-37497729 | None:intergenic |
| AGGGAAAGAACCTGAATTGT+TGG | 0.436427 | 5.1:-37499039 | None:intergenic |
| AACATATCGTTCTGGAACTT+TGG | 0.450602 | 5.1:-37497755 | None:intergenic |
| TCGTTCTGGAACTTTGGTGA+TGG | 0.474377 | 5.1:-37497749 | None:intergenic |
| TAGATACAACTAGAGGGTCT+TGG | 0.489914 | 5.1:-37497785 | None:intergenic |
| TTTGGATTTCTCATGTATGA+TGG | 0.500988 | 5.1:-37498998 | None:intergenic |
| AAAGAAACCCCATTCTCTGC+AGG | 0.510694 | 5.1:-37497898 | None:intergenic |
| GAAGCTTGGAAGCTCTCTCT+TGG | 0.514547 | 5.1:+37497696 | None:intergenic |
| CGTTCTGGAACTTTGGTGAT+GGG | 0.517955 | 5.1:-37497748 | None:intergenic |
| AGAGGATCACAAATTGGAAT+GGG | 0.518486 | 5.1:+37498949 | MS.gene59002:CDS |
| CCAAAACCTTCCATATCACC+TGG | 0.521789 | 5.1:-37498908 | None:intergenic |
| CAGAGGATCACAAATTGGAA+TGG | 0.527609 | 5.1:+37498948 | MS.gene59002:CDS |
| AACTTATTCAAATCGATGAC+TGG | 0.529324 | 5.1:-37497831 | None:intergenic |
| CGGAGTCAATCCTTTATTGG+TGG | 0.547582 | 5.1:+37498814 | MS.gene59002:CDS |
| TTTACTATCTCCTGGACAGA+AGG | 0.556430 | 5.1:-37497721 | None:intergenic |
| TATTGGCAGCTGATTAATCA+CGG | 0.589335 | 5.1:+37498794 | MS.gene59002:intron |
| GATCATGCCTGCAGAGAATG+GGG | 0.590145 | 5.1:+37497891 | MS.gene59002:CDS |
| TCAACTGTTTGTGGTATCAG+AGG | 0.600452 | 5.1:+37498931 | MS.gene59002:CDS |
| CAGAAACCAGGTGATATGGA+AGG | 0.617232 | 5.1:+37498902 | MS.gene59002:CDS |
| AAATCGATGACTGGAACTTG+TGG | 0.617267 | 5.1:-37497822 | None:intergenic |
| TTTGTGCTAGATACAACTAG+AGG | 0.618176 | 5.1:-37497792 | None:intergenic |
| AAGTTGTTGTCAGAAGAAGA+TGG | 0.623206 | 5.1:+37497849 | MS.gene59002:CDS |
| GGAAAGAACCTGAATTGTTG+GGG | 0.633271 | 5.1:-37499037 | None:intergenic |
| TTGTGCTAGATACAACTAGA+GGG | 0.634845 | 5.1:-37497791 | None:intergenic |
| AGACTTCTTCAATCTACCAG+TGG | 0.636943 | 5.1:+37498859 | MS.gene59002:CDS |
| AAAATATTATCTCAGAAACC+AGG | 0.664490 | 5.1:+37498890 | MS.gene59002:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAAAAAAAAAAAAAAAAA+AGG | - | chr5.1:37497942-37497961 | None:intergenic | 0.0% |
| !! | AAAAAAAAAAAAAAAGGAGA+CGG | - | chr5.1:37497937-37497956 | None:intergenic | 15.0% |
| !! | AAAATAATCCATAAATATCT+CGG | - | chr5.1:37498374-37498393 | None:intergenic | 15.0% |
| !! | ATTTAGAAAATAGCAAAATA+AGG | + | chr5.1:37498055-37498074 | MS.gene59002:intron | 15.0% |
| !! | TAGAAATATATGTTAATAAG+TGG | + | chr5.1:37498129-37498148 | MS.gene59002:intron | 15.0% |
| !!! | AGTTCATGAAATATATATTT+TGG | + | chr5.1:37498262-37498281 | MS.gene59002:intron | 15.0% |
| !!! | ATGATATTTCTAATTATTAG+TGG | - | chr5.1:37498104-37498123 | None:intergenic | 15.0% |
| !!! | ATTAATGATCGAAATTTTTT+AGG | + | chr5.1:37498182-37498201 | MS.gene59002:intron | 15.0% |
| !!! | ATTATGGTTTTATTGTAATT+TGG | + | chr5.1:37498730-37498749 | MS.gene59002:intron | 15.0% |
| !!! | ATTGATATAGACTTATTTTT+TGG | + | chr5.1:37498671-37498690 | MS.gene59002:intron | 15.0% |
| !!! | TCATACTTCATTATTTTAAA+AGG | - | chr5.1:37498222-37498241 | None:intergenic | 15.0% |
| !!! | TGATATTTCTAATTATTAGT+GGG | - | chr5.1:37498103-37498122 | None:intergenic | 15.0% |
| !!! | TTAATGATCGAAATTTTTTA+GGG | + | chr5.1:37498183-37498202 | MS.gene59002:intron | 15.0% |
| !!! | TTATGGTTTTATTGTAATTT+GGG | + | chr5.1:37498731-37498750 | MS.gene59002:intron | 15.0% |
| !!! | TTTTAGTTGTATCATATTTA+TGG | + | chr5.1:37498285-37498304 | MS.gene59002:intron | 15.0% |
| !! | AAAATCATGGTTTATGTTAT+GGG | - | chr5.1:37498769-37498788 | None:intergenic | 20.0% |
| !! | TTTATAACCAAAATTTATCG+AGG | + | chr5.1:37498314-37498333 | MS.gene59002:intron | 20.0% |
| !!! | AACCATGATTTTGAAATTAT+TGG | + | chr5.1:37498777-37498796 | MS.gene59002:intron | 20.0% |
| !!! | AATTTGTTCTACTTTGTAAA+CGG | + | chr5.1:37498509-37498528 | MS.gene59002:intron | 20.0% |
| !!! | CCGAAATATAAATTTTAGAA+CGG | + | chr5.1:37498452-37498471 | MS.gene59002:intron | 20.0% |
| !!! | CCGTTCTAAAATTTATATTT+CGG | - | chr5.1:37498455-37498474 | None:intergenic | 20.0% |
| !!! | TTAAAATTTTCCACCAATAA+AGG | - | chr5.1:37498827-37498846 | None:intergenic | 20.0% |
| !!! | TTTTCACTTATTCAAAAGAA+CGG | + | chr5.1:37497970-37497989 | MS.gene59002:intron | 20.0% |
| ! | AAAATATTATCTCAGAAACC+AGG | + | chr5.1:37498890-37498909 | MS.gene59002:CDS | 25.0% |
| ! | CAAAATCATGGTTTATGTTA+TGG | - | chr5.1:37498770-37498789 | None:intergenic | 25.0% |
| ! | TCTCAATGGTTACATAATTA+TGG | + | chr5.1:37498714-37498733 | MS.gene59002:intron | 25.0% |
| ! | TGCCAATAATTTCAAAATCA+TGG | - | chr5.1:37498782-37498801 | None:intergenic | 25.0% |
| !!! | AATGGTTTTGAAAGAAAGAT+TGG | + | chr5.1:37498025-37498044 | MS.gene59002:intron | 25.0% |
| AACTTATTCAAATCGATGAC+TGG | - | chr5.1:37497834-37497853 | None:intergenic | 30.0% | |
| AAGCTAAACATTCAAAGACT+TGG | + | chr5.1:37498399-37498418 | MS.gene59002:intron | 30.0% | |
| AAGGACATTGTCTAAAAGAA+TGG | + | chr5.1:37498007-37498026 | MS.gene59002:intron | 30.0% | |
| AGAGACTTATGTAATCTCAA+TGG | + | chr5.1:37498700-37498719 | MS.gene59002:intron | 30.0% | |
| ATTTACAACAGTAGAAAGAG+AGG | + | chr5.1:37498479-37498498 | MS.gene59002:intron | 30.0% | |
| GGAATACTAGGAAATAAGTT+TGG | - | chr5.1:37499019-37499038 | None:intergenic | 30.0% | |
| GTTTGAGTCATACTTCATAA+GGG | + | chr5.1:37498531-37498550 | MS.gene59002:intron | 30.0% | |
| TTATAAATACCCATCGCTTT+CGG | + | chr5.1:37498605-37498624 | MS.gene59002:intron | 30.0% | |
| TTTGGATTTCTCATGTATGA+TGG | - | chr5.1:37499001-37499020 | None:intergenic | 30.0% | |
| ! | AATATTTTCTTCTCTTCCAC+TGG | - | chr5.1:37498878-37498897 | None:intergenic | 30.0% |
| AAGTTGTTGTCAGAAGAAGA+TGG | + | chr5.1:37497849-37497868 | MS.gene59002:CDS | 35.0% | |
| AATACGCTCCGAGATATTTA+TGG | + | chr5.1:37498363-37498382 | MS.gene59002:intron | 35.0% | |
| AGAGGATCACAAATTGGAAT+GGG | + | chr5.1:37498949-37498968 | MS.gene59002:CDS | 35.0% | |
| GGTTTGAGTCATACTTCATA+AGG | + | chr5.1:37498530-37498549 | MS.gene59002:intron | 35.0% | |
| TATTGGCAGCTGATTAATCA+CGG | + | chr5.1:37498794-37498813 | MS.gene59002:intron | 35.0% | |
| TTGTGCTAGATACAACTAGA+GGG | - | chr5.1:37497794-37497813 | None:intergenic | 35.0% | |
| TTTGATATTCCGAAAGCGAT+GGG | - | chr5.1:37498617-37498636 | None:intergenic | 35.0% | |
| TTTGTGCTAGATACAACTAG+AGG | - | chr5.1:37497795-37497814 | None:intergenic | 35.0% | |
| ! | GTTTTGTGAACATATCGTTC+TGG | - | chr5.1:37497766-37497785 | None:intergenic | 35.0% |
| ! | TTTTGATATTCCGAAAGCGA+TGG | - | chr5.1:37498618-37498637 | None:intergenic | 35.0% |
| !! | AACATATCGTTCTGGAACTT+TGG | - | chr5.1:37497758-37497777 | None:intergenic | 35.0% |
| AAATCGATGACTGGAACTTG+TGG | - | chr5.1:37497825-37497844 | None:intergenic | 40.0% | |
| AACGGTTGTGTGTTTGACAA+AGG | + | chr5.1:37497988-37498007 | MS.gene59002:intron | 40.0% | |
| AGACTTCTTCAATCTACCAG+TGG | + | chr5.1:37498859-37498878 | MS.gene59002:CDS | 40.0% | |
| ATCTCAGAAACCAGGTGATA+TGG | + | chr5.1:37498898-37498917 | MS.gene59002:CDS | 40.0% | |
| CAGAGGATCACAAATTGGAA+TGG | + | chr5.1:37498948-37498967 | MS.gene59002:CDS | 40.0% | |
| CTGAATTGTTGGGGAATACT+AGG | - | chr5.1:37499031-37499050 | None:intergenic | 40.0% | |
| GGTATCAGAGGATCACAAAT+TGG | + | chr5.1:37498943-37498962 | MS.gene59002:CDS | 40.0% | |
| TAGATACAACTAGAGGGTCT+TGG | - | chr5.1:37497788-37497807 | None:intergenic | 40.0% | |
| TCAACTGTTTGTGGTATCAG+AGG | + | chr5.1:37498931-37498950 | MS.gene59002:CDS | 40.0% | |
| TCACGGAGTCAATCCTTTAT+TGG | + | chr5.1:37498811-37498830 | MS.gene59002:CDS | 40.0% | |
| TTTACTATCTCCTGGACAGA+AGG | - | chr5.1:37497724-37497743 | None:intergenic | 40.0% | |
| ! | TGCTCGTCCTCGATAAATTT+TGG | - | chr5.1:37498324-37498343 | None:intergenic | 40.0% |
| !! | AGGTTTTGGTCAACTGTTTG+TGG | + | chr5.1:37498922-37498941 | MS.gene59002:CDS | 40.0% |
| AAAGAAACCCCATTCTCTGC+AGG | - | chr5.1:37497901-37497920 | None:intergenic | 45.0% | |
| CAGAAACCAGGTGATATGGA+AGG | + | chr5.1:37498902-37498921 | MS.gene59002:CDS | 45.0% | |
| CAGAGAATGGGGTTTCTTTC+AGG | + | chr5.1:37497902-37497921 | MS.gene59002:CDS | 45.0% | |
| CCAAAACCTTCCATATCACC+TGG | - | chr5.1:37498911-37498930 | None:intergenic | 45.0% | |
| CGGAGTCAATCCTTTATTGG+TGG | + | chr5.1:37498814-37498833 | MS.gene59002:CDS | 45.0% | |
| GGTGCGATGCTATCATTAGT+TGG | + | chr5.1:37498578-37498597 | MS.gene59002:intron | 45.0% | |
| TGATCATGCCTGCAGAGAAT+GGG | + | chr5.1:37497890-37497909 | MS.gene59002:CDS | 45.0% | |
| TTGATCATGCCTGCAGAGAA+TGG | + | chr5.1:37497889-37497908 | MS.gene59002:CDS | 45.0% | |
| ! | CCAGGTGATATGGAAGGTTT+TGG | + | chr5.1:37498908-37498927 | MS.gene59002:CDS | 45.0% |
| ! | TGGGTTGCTTTACTATCTCC+TGG | - | chr5.1:37497732-37497751 | None:intergenic | 45.0% |
| !! | CGTTCTGGAACTTTGGTGAT+GGG | - | chr5.1:37497751-37497770 | None:intergenic | 45.0% |
| !! | TCGTTCTGGAACTTTGGTGA+TGG | - | chr5.1:37497752-37497771 | None:intergenic | 45.0% |
| GAGTTCAACTCTCGTTGCTC+TGG | + | chr5.1:37498557-37498576 | MS.gene59002:intron | 50.0% | |
| GATCATGCCTGCAGAGAATG+GGG | + | chr5.1:37497891-37497910 | MS.gene59002:CDS | 50.0% | |
| !! | TCTCTTGGTGCCTTCTGTCC+AGG | + | chr5.1:37497711-37497730 | MS.gene59002:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 37497700 | 37499050 | 37497700 | ID=MS.gene59002 |
| chr5.1 | mRNA | 37497700 | 37499050 | 37497700 | ID=MS.gene59002.t1;Parent=MS.gene59002 |
| chr5.1 | exon | 37497700 | 37497923 | 37497700 | ID=MS.gene59002.t1.exon1;Parent=MS.gene59002.t1 |
| chr5.1 | CDS | 37497700 | 37497923 | 37497700 | ID=cds.MS.gene59002.t1;Parent=MS.gene59002.t1 |
| chr5.1 | exon | 37498803 | 37499050 | 37498803 | ID=MS.gene59002.t1.exon2;Parent=MS.gene59002.t1 |
| chr5.1 | CDS | 37498803 | 37499050 | 37498803 | ID=cds.MS.gene59002.t1;Parent=MS.gene59002.t1 |
| Gene Sequence |
| Protein sequence |
