Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61626.t1 | XP_013455693.1 | 96.8 | 317 | 10 | 0 | 1 | 317 | 1 | 317 | 3.50E-181 | 644 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61626.t1 | Q9FG13 | 49.5 | 317 | 153 | 4 | 5 | 315 | 10 | 325 | 1.1e-93 | 344.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61626.t1 | A0A072UIY5 | 96.8 | 317 | 10 | 0 | 1 | 317 | 1 | 317 | 2.6e-181 | 644.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050082 | MS.gene61626 | 0.813095 | 3.04E-51 | -1.69E-46 |
| MS.gene050165 | MS.gene61626 | -0.805382 | 1.37E-49 | -1.69E-46 |
| MS.gene050338 | MS.gene61626 | 0.801986 | 6.95E-49 | -1.69E-46 |
| MS.gene050444 | MS.gene61626 | -0.810362 | 1.20E-50 | -1.69E-46 |
| MS.gene050496 | MS.gene61626 | -0.811041 | 8.54E-51 | -1.69E-46 |
| MS.gene050504 | MS.gene61626 | -0.814999 | 1.16E-51 | -1.69E-46 |
| MS.gene050612 | MS.gene61626 | -0.824995 | 5.97E-54 | -1.69E-46 |
| MS.gene05079 | MS.gene61626 | 0.810916 | 9.09E-51 | -1.69E-46 |
| MS.gene051138 | MS.gene61626 | 0.8114 | 7.13E-51 | -1.69E-46 |
| MS.gene051364 | MS.gene61626 | 0.808044 | 3.76E-50 | -1.69E-46 |
| MS.gene051366 | MS.gene61626 | 0.834945 | 2.23E-56 | -1.69E-46 |
| MS.gene051367 | MS.gene61626 | 0.807776 | 4.28E-50 | -1.69E-46 |
| MS.gene051651 | MS.gene61626 | 0.806924 | 6.49E-50 | -1.69E-46 |
| MS.gene052129 | MS.gene61626 | 0.813836 | 2.09E-51 | -1.69E-46 |
| MS.gene054954 | MS.gene61626 | -0.813034 | 3.14E-51 | -1.69E-46 |
| MS.gene055204 | MS.gene61626 | 0.804698 | 1.91E-49 | -1.69E-46 |
| MS.gene055748 | MS.gene61626 | -0.800223 | 1.59E-48 | -1.69E-46 |
| MS.gene055751 | MS.gene61626 | -0.81813 | 2.30E-52 | -1.69E-46 |
| MS.gene055776 | MS.gene61626 | -0.827276 | 1.71E-54 | -1.69E-46 |
| MS.gene055942 | MS.gene61626 | 0.816898 | 4.36E-52 | -1.69E-46 |
| MS.gene056198 | MS.gene61626 | 0.815755 | 7.86E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene61626 | -0.822227 | 2.65E-53 | -1.69E-46 |
| MS.gene056856 | MS.gene61626 | 0.81986 | 9.31E-53 | -1.69E-46 |
| MS.gene056857 | MS.gene61626 | 0.810155 | 1.33E-50 | -1.69E-46 |
| MS.gene057768 | MS.gene61626 | 0.802486 | 5.48E-49 | -1.69E-46 |
| MS.gene058268 | MS.gene61626 | 0.800445 | 1.44E-48 | -1.69E-46 |
| MS.gene059022 | MS.gene61626 | -0.811967 | 5.37E-51 | -1.69E-46 |
| MS.gene059677 | MS.gene61626 | -0.808489 | 3.02E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene61626 | -0.803587 | 3.25E-49 | -1.69E-46 |
| MS.gene060762 | MS.gene61626 | -0.810624 | 1.05E-50 | -1.69E-46 |
| MS.gene060823 | MS.gene61626 | 0.817259 | 3.62E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61626.t1 | MTR_4g050140 | 96.845 | 317 | 10 | 0 | 1 | 317 | 1 | 317 | 0.0 | 636 |
| MS.gene61626.t1 | MTR_7g107040 | 58.013 | 312 | 128 | 3 | 5 | 315 | 7 | 316 | 6.34e-128 | 368 |
| MS.gene61626.t1 | MTR_3g012440 | 36.550 | 342 | 190 | 7 | 2 | 318 | 4 | 343 | 5.75e-66 | 211 |
| MS.gene61626.t1 | MTR_1g111280 | 38.226 | 327 | 178 | 10 | 4 | 315 | 25 | 342 | 9.02e-65 | 208 |
| MS.gene61626.t1 | MTR_7g406880 | 34.462 | 325 | 195 | 7 | 11 | 317 | 27 | 351 | 1.24e-60 | 197 |
| MS.gene61626.t1 | MTR_7g056207 | 34.568 | 324 | 191 | 7 | 2 | 315 | 37 | 349 | 1.27e-55 | 184 |
| MS.gene61626.t1 | MTR_4g086510 | 33.333 | 324 | 195 | 7 | 2 | 315 | 5 | 317 | 9.32e-54 | 179 |
| MS.gene61626.t1 | MTR_4g086480 | 33.837 | 331 | 187 | 8 | 2 | 316 | 5 | 319 | 3.04e-52 | 174 |
| MS.gene61626.t1 | MTR_3g101420 | 33.968 | 315 | 185 | 8 | 13 | 317 | 15 | 316 | 7.77e-52 | 173 |
| MS.gene61626.t1 | MTR_1g102170 | 38.550 | 262 | 152 | 4 | 40 | 298 | 68 | 323 | 1.10e-50 | 171 |
| MS.gene61626.t1 | MTR_1g012430 | 35.714 | 322 | 185 | 12 | 4 | 317 | 2 | 309 | 5.29e-50 | 169 |
| MS.gene61626.t1 | MTR_1g082210 | 34.796 | 319 | 177 | 6 | 18 | 318 | 38 | 343 | 2.54e-49 | 168 |
| MS.gene61626.t1 | MTR_1g105020 | 33.642 | 324 | 199 | 8 | 2 | 315 | 5 | 322 | 1.42e-48 | 165 |
| MS.gene61626.t1 | MTR_6g065400 | 34.185 | 313 | 184 | 5 | 18 | 317 | 32 | 335 | 3.53e-48 | 164 |
| MS.gene61626.t1 | MTR_4g086470 | 35.806 | 310 | 187 | 6 | 12 | 317 | 18 | 319 | 1.69e-47 | 162 |
| MS.gene61626.t1 | MTR_6g065270 | 32.075 | 318 | 190 | 5 | 17 | 317 | 29 | 337 | 2.48e-47 | 162 |
| MS.gene61626.t1 | MTR_6g065370 | 33.227 | 313 | 187 | 5 | 18 | 317 | 32 | 335 | 2.74e-47 | 162 |
| MS.gene61626.t1 | MTR_8g035520 | 35.082 | 305 | 189 | 6 | 18 | 317 | 26 | 326 | 2.28e-46 | 159 |
| MS.gene61626.t1 | MTR_1g089310 | 34.688 | 320 | 176 | 7 | 18 | 318 | 54 | 359 | 3.50e-46 | 160 |
| MS.gene61626.t1 | MTR_1g089310 | 34.688 | 320 | 176 | 7 | 18 | 318 | 38 | 343 | 4.09e-46 | 159 |
| MS.gene61626.t1 | MTR_6g065110 | 33.569 | 283 | 170 | 4 | 42 | 315 | 61 | 334 | 1.19e-45 | 158 |
| MS.gene61626.t1 | MTR_5g005900 | 33.333 | 327 | 201 | 9 | 3 | 317 | 7 | 328 | 1.44e-44 | 155 |
| MS.gene61626.t1 | MTR_1g102160 | 34.266 | 286 | 173 | 6 | 38 | 315 | 47 | 325 | 3.72e-44 | 154 |
| MS.gene61626.t1 | MTR_6g464510 | 33.922 | 283 | 175 | 6 | 38 | 315 | 51 | 326 | 5.39e-44 | 153 |
| MS.gene61626.t1 | MTR_0007s0520 | 33.779 | 299 | 179 | 9 | 15 | 302 | 23 | 313 | 7.32e-44 | 153 |
| MS.gene61626.t1 | MTR_3g450680 | 31.818 | 330 | 204 | 9 | 1 | 317 | 12 | 333 | 1.70e-43 | 152 |
| MS.gene61626.t1 | MTR_7g093950 | 36.680 | 259 | 151 | 6 | 63 | 318 | 101 | 349 | 2.16e-43 | 152 |
| MS.gene61626.t1 | MTR_6g464280 | 33.651 | 315 | 189 | 9 | 12 | 315 | 19 | 324 | 2.85e-43 | 151 |
| MS.gene61626.t1 | MTR_8g035540 | 33.442 | 308 | 192 | 6 | 17 | 315 | 13 | 316 | 4.43e-43 | 151 |
| MS.gene61626.t1 | MTR_6g065120 | 32.517 | 286 | 174 | 4 | 42 | 317 | 61 | 337 | 1.44e-42 | 150 |
| MS.gene61626.t1 | MTR_6g464320 | 33.333 | 288 | 172 | 8 | 39 | 315 | 47 | 325 | 2.26e-42 | 149 |
| MS.gene61626.t1 | MTR_6g065430 | 33.094 | 278 | 175 | 4 | 43 | 318 | 62 | 330 | 3.21e-42 | 149 |
| MS.gene61626.t1 | MTR_5g005850 | 31.804 | 327 | 202 | 11 | 2 | 315 | 10 | 328 | 3.52e-42 | 149 |
| MS.gene61626.t1 | MTR_4g086540 | 31.250 | 320 | 184 | 7 | 2 | 315 | 15 | 304 | 2.72e-41 | 146 |
| MS.gene61626.t1 | MTR_6g065340 | 32.982 | 285 | 157 | 6 | 42 | 317 | 71 | 330 | 4.33e-41 | 146 |
| MS.gene61626.t1 | MTR_5g005920 | 32.727 | 330 | 201 | 11 | 2 | 317 | 10 | 332 | 6.23e-41 | 145 |
| MS.gene61626.t1 | MTR_8g040860 | 29.907 | 321 | 211 | 8 | 5 | 317 | 16 | 330 | 2.24e-40 | 144 |
| MS.gene61626.t1 | MTR_8g035500 | 29.643 | 280 | 185 | 5 | 42 | 315 | 44 | 317 | 3.51e-38 | 138 |
| MS.gene61626.t1 | MTR_6g464120 | 37.129 | 202 | 120 | 3 | 39 | 238 | 51 | 247 | 5.75e-38 | 141 |
| MS.gene61626.t1 | MTR_6g022710 | 31.579 | 266 | 144 | 4 | 66 | 298 | 158 | 418 | 3.08e-33 | 127 |
| MS.gene61626.t1 | MTR_7g051020 | 29.630 | 324 | 215 | 7 | 2 | 317 | 9 | 327 | 6.53e-33 | 124 |
| MS.gene61626.t1 | MTR_1g104933 | 31.707 | 328 | 198 | 10 | 1 | 317 | 6 | 318 | 5.82e-32 | 121 |
| MS.gene61626.t1 | MTR_1g104930 | 29.448 | 326 | 210 | 7 | 2 | 317 | 9 | 324 | 5.96e-32 | 121 |
| MS.gene61626.t1 | MTR_0007s0480 | 29.114 | 158 | 102 | 5 | 153 | 302 | 4 | 159 | 4.94e-12 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene61626.t1 | AT5G06570 | 49.527 | 317 | 153 | 4 | 5 | 315 | 25 | 340 | 1.78e-106 | 314 |
| MS.gene61626.t1 | AT5G06570 | 49.527 | 317 | 153 | 4 | 5 | 315 | 25 | 340 | 1.78e-106 | 314 |
| MS.gene61626.t1 | AT5G06570 | 49.527 | 317 | 153 | 4 | 5 | 315 | 10 | 325 | 2.28e-106 | 313 |
| MS.gene61626.t1 | AT5G06570 | 49.527 | 317 | 153 | 4 | 5 | 315 | 10 | 325 | 2.28e-106 | 313 |
| MS.gene61626.t1 | AT5G16080 | 34.985 | 323 | 191 | 9 | 5 | 316 | 28 | 342 | 1.49e-56 | 186 |
| MS.gene61626.t1 | AT5G62180 | 38.019 | 313 | 169 | 9 | 18 | 315 | 21 | 323 | 1.83e-53 | 178 |
| MS.gene61626.t1 | AT1G68620 | 35.625 | 320 | 186 | 8 | 5 | 316 | 23 | 330 | 4.30e-53 | 177 |
| MS.gene61626.t1 | AT5G27320 | 37.143 | 315 | 168 | 8 | 18 | 315 | 38 | 339 | 4.75e-53 | 177 |
| MS.gene61626.t1 | AT3G05120 | 34.385 | 317 | 176 | 6 | 18 | 315 | 38 | 341 | 1.39e-49 | 168 |
| MS.gene61626.t1 | AT3G63010 | 35.312 | 320 | 171 | 7 | 18 | 316 | 38 | 342 | 2.39e-49 | 168 |
| MS.gene61626.t1 | AT1G47480 | 30.195 | 308 | 200 | 6 | 13 | 317 | 16 | 311 | 2.01e-47 | 162 |
| MS.gene61626.t1 | AT2G45600 | 34.783 | 276 | 161 | 7 | 17 | 283 | 20 | 285 | 5.95e-46 | 159 |
| MS.gene61626.t1 | AT5G23530 | 34.098 | 305 | 185 | 6 | 18 | 317 | 35 | 328 | 1.20e-44 | 155 |
| MS.gene61626.t1 | AT3G48690 | 32.407 | 324 | 208 | 7 | 1 | 316 | 1 | 321 | 9.14e-43 | 150 |
| MS.gene61626.t1 | AT2G45610 | 32.143 | 280 | 181 | 4 | 42 | 316 | 49 | 324 | 2.80e-42 | 149 |
| MS.gene61626.t1 | AT3G48700 | 31.231 | 333 | 205 | 9 | 1 | 316 | 1 | 326 | 7.80e-42 | 148 |
| MS.gene61626.t1 | AT1G19190 | 28.435 | 313 | 206 | 7 | 13 | 317 | 13 | 315 | 9.55e-40 | 142 |
| MS.gene61626.t1 | AT3G27320 | 33.692 | 279 | 148 | 6 | 55 | 298 | 120 | 396 | 1.55e-36 | 136 |
| MS.gene61626.t1 | AT3G27320 | 33.692 | 279 | 148 | 6 | 55 | 298 | 152 | 428 | 2.57e-36 | 136 |
| MS.gene61626.t1 | AT2G03550 | 28.221 | 326 | 211 | 8 | 1 | 317 | 1 | 312 | 3.25e-36 | 132 |
| MS.gene61626.t1 | AT5G14310 | 30.201 | 298 | 169 | 4 | 57 | 315 | 140 | 437 | 1.86e-34 | 130 |
| MS.gene61626.t1 | AT1G49650 | 30.868 | 311 | 199 | 9 | 13 | 316 | 69 | 370 | 2.71e-34 | 129 |
| MS.gene61626.t1 | AT1G49640 | 30.128 | 312 | 197 | 10 | 13 | 315 | 14 | 313 | 1.72e-32 | 123 |
| MS.gene61626.t1 | AT1G49660 | 30.104 | 289 | 176 | 8 | 1 | 276 | 1 | 276 | 4.22e-31 | 119 |
Find 70 sgRNAs with CRISPR-Local
Find 109 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTCAAGGCTCTTACTTCT+TGG | 0.253436 | 4.1:-47678706 | None:intergenic |
| AGTTGGCACCGGTTCGGGTT+AGG | 0.267807 | 4.1:+47678108 | MS.gene61626:CDS |
| CATAATTTGGCGGTTCGGTT+TGG | 0.283309 | 4.1:+47678071 | MS.gene61626:CDS |
| TCTCCAACCACCACAAGAAT+TGG | 0.284897 | 4.1:-47678742 | None:intergenic |
| ATAATTTGGCGGTTCGGTTT+GGG | 0.314873 | 4.1:+47678072 | MS.gene61626:CDS |
| CAATTCATAGAGAAGAATTA+AGG | 0.314893 | 4.1:+47678922 | MS.gene61626:CDS |
| GTTGGCACCGGTTCGGGTTA+GGG | 0.314906 | 4.1:+47678109 | MS.gene61626:CDS |
| CTCTTACTTCTTGGTCCAAA+TGG | 0.330488 | 4.1:-47678697 | None:intergenic |
| GGTTGAGTTGGCACCGGTTC+GGG | 0.335939 | 4.1:+47678103 | MS.gene61626:CDS |
| AAAATGATTTGCTTAAAGAT+AGG | 0.343018 | 4.1:+47678764 | MS.gene61626:CDS |
| TGGTAATATAGCTCATAATT+TGG | 0.349968 | 4.1:+47678058 | MS.gene61626:CDS |
| TTGGCGGTTCGGTTTGGGTT+TGG | 0.380867 | 4.1:+47678077 | MS.gene61626:CDS |
| CGGTTGAGTTGGCACCGGTT+CGG | 0.381756 | 4.1:+47678102 | MS.gene61626:CDS |
| CATCCACTTGTGAATCCATT+TGG | 0.383792 | 4.1:+47678682 | MS.gene61626:CDS |
| TGAACTGTGATGTTGAAGGC+TGG | 0.384844 | 4.1:-47677654 | None:intergenic |
| ATCGACTTGCGCCAGAGAAT+CGG | 0.412033 | 4.1:+47677901 | MS.gene61626:CDS |
| CTCCAACCACCACAAGAATT+GGG | 0.412421 | 4.1:-47678741 | None:intergenic |
| TGCTATTGAAGACGGTTTCA+TGG | 0.425705 | 4.1:+47677932 | MS.gene61626:CDS |
| TAGCTCATAATTTGGCGGTT+CGG | 0.429884 | 4.1:+47678066 | MS.gene61626:CDS |
| TTTATTTCGGGTGACTCGGC+TGG | 0.436373 | 4.1:+47678035 | MS.gene61626:CDS |
| TTGGCACCGGTTCGGGTTAG+GGG | 0.442158 | 4.1:+47678110 | MS.gene61626:CDS |
| ATAGAGTATGTAGAATTTGA+AGG | 0.444698 | 4.1:+47678829 | MS.gene61626:CDS |
| GGGCAGAGGATTATGCAAAA+AGG | 0.446914 | 4.1:+47678785 | MS.gene61626:CDS |
| CGGTCCTTCAGCTTCTGACT+TGG | 0.447400 | 4.1:-47678174 | None:intergenic |
| GCACATGGAAGCCGATTCTC+TGG | 0.447935 | 4.1:-47677912 | None:intergenic |
| AATTCATAGAGAAGAATTAA+GGG | 0.450220 | 4.1:+47678923 | MS.gene61626:CDS |
| GGTTCGGTTTGGGTTTGGTT+CGG | 0.452189 | 4.1:+47678082 | MS.gene61626:CDS |
| AGCTTCTGACTTGGTCCTCA+CGG | 0.456270 | 4.1:-47678165 | None:intergenic |
| ACATAACCCCTAACCCGAAC+CGG | 0.456317 | 4.1:-47678116 | None:intergenic |
| TGGGTTTGGTTCGGTTGAGT+TGG | 0.466016 | 4.1:+47678091 | MS.gene61626:CDS |
| TGGGTTTAACGTGCGAACTA+TGG | 0.478396 | 4.1:-47677634 | None:intergenic |
| GAACTGTGATGTTGAAGGCT+GGG | 0.480754 | 4.1:-47677653 | None:intergenic |
| AAATGATTTGCTTAAAGATA+GGG | 0.489878 | 4.1:+47678765 | MS.gene61626:CDS |
| CACGGTGGCGGCTTCTGCAT+CGG | 0.491547 | 4.1:+47677798 | MS.gene61626:CDS |
| ATGAGTTAGCTGGCTTATAG+AGG | 0.492426 | 4.1:-47677740 | None:intergenic |
| TCAACTTGGAACTTATTGAC+AGG | 0.502436 | 4.1:+47678210 | MS.gene61626:CDS |
| TCTGGCGCAAGTCGATAGTC+AGG | 0.504250 | 4.1:-47677894 | None:intergenic |
| ACGGTTTCATGGCTGTTAAG+TGG | 0.505255 | 4.1:+47677943 | MS.gene61626:CDS |
| GTCTTGAACTGTGATGTTGA+AGG | 0.511456 | 4.1:-47677658 | None:intergenic |
| CTTCCATGTGCTATTGAAGA+CGG | 0.514278 | 4.1:+47677924 | MS.gene61626:CDS |
| CCGTGAATGTAGTAAAAGAT+TGG | 0.516981 | 4.1:-47677780 | None:intergenic |
| TCTGCATCGGCTCACGAACA+TGG | 0.517704 | 4.1:+47677811 | MS.gene61626:CDS |
| GACCCAATTCTTGTGGTGGT+TGG | 0.518707 | 4.1:+47678739 | MS.gene61626:CDS |
| TGAAGAGTGAGATGGATCCT+TGG | 0.522474 | 4.1:+47677982 | MS.gene61626:CDS |
| AGAAGCTTTGAAGAGTGAGA+TGG | 0.536702 | 4.1:+47677974 | MS.gene61626:CDS |
| CAAGTCAGAAGCTGAAGGAC+CGG | 0.553703 | 4.1:+47678175 | MS.gene61626:CDS |
| ATGATCAGTATTCTCTCCGA+TGG | 0.556242 | 4.1:-47678662 | None:intergenic |
| AAGTTGAGAAAAGCATCATC+CGG | 0.562536 | 4.1:-47678194 | None:intergenic |
| TGGTTCGGTTGAGTTGGCAC+CGG | 0.564253 | 4.1:+47678097 | MS.gene61626:CDS |
| GTTCTTCATGTCTATAACGA+CGG | 0.568020 | 4.1:+47677609 | MS.gene61626:CDS |
| ATTTCGGGTGACTCGGCTGG+TGG | 0.569148 | 4.1:+47678038 | MS.gene61626:CDS |
| TCTTGACCCAATTCTTGTGG+TGG | 0.585357 | 4.1:+47678735 | MS.gene61626:CDS |
| GAATTTGAAGGACAACAACA+TGG | 0.593809 | 4.1:+47678841 | MS.gene61626:CDS |
| TAGCTGGCTTATAGAGGCGA+AGG | 0.596667 | 4.1:-47677734 | None:intergenic |
| TTTGCTTAAAGATAGGGCAG+AGG | 0.596874 | 4.1:+47678771 | MS.gene61626:CDS |
| TAATATAGCTCATAATTTGG+CGG | 0.598417 | 4.1:+47678061 | MS.gene61626:CDS |
| ACCATCAAAAGAGTTGATGT+TGG | 0.606485 | 4.1:+47678891 | MS.gene61626:CDS |
| GGTCAAGATCAATTTCCTCA+AGG | 0.607533 | 4.1:-47678720 | None:intergenic |
| CCCGGTTAAAATCAGCCACA+TGG | 0.609681 | 4.1:-47678010 | None:intergenic |
| TGGAGACTTTCTATACCCAT+CGG | 0.612006 | 4.1:+47678646 | MS.gene61626:CDS |
| TGATCTTGACCCAATTCTTG+TGG | 0.614564 | 4.1:+47678732 | MS.gene61626:CDS |
| TGATCAGTATTCTCTCCGAT+GGG | 0.634036 | 4.1:-47678661 | None:intergenic |
| AGGACCAAGTCAGAAGCTGA+AGG | 0.644457 | 4.1:+47678170 | MS.gene61626:CDS |
| ACTATAGTTGACGAATGTCG+TGG | 0.650039 | 4.1:+47677585 | MS.gene61626:CDS |
| GGTCCAAATGGATTCACAAG+TGG | 0.650243 | 4.1:-47678685 | None:intergenic |
| AAACCGTCTTCAATAGCACA+TGG | 0.659267 | 4.1:-47677927 | None:intergenic |
| TCAGCCACATGGCTCAACCA+AGG | 0.660011 | 4.1:-47677999 | None:intergenic |
| GGATCCTTGGTTGAGCCATG+TGG | 0.675420 | 4.1:+47677995 | MS.gene61626:CDS |
| AATTTGAAGGACAACAACAT+GGG | 0.677406 | 4.1:+47678842 | MS.gene61626:CDS |
| ACCAAGAAGTAAGAGCCTTG+AGG | 0.706738 | 4.1:+47678705 | MS.gene61626:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAAAAAATTAAATCATTAT+CGG | - | chr4.1:47678451-47678470 | None:intergenic | 10.0% |
| !!! | TATAAAAAGTGTCAAAATTA+TGG | + | chr4.1:47678543-47678562 | MS.gene61626:intron | 15.0% |
| !! | CTTGCATAATGTAAAATATT+TGG | - | chr4.1:47678271-47678290 | None:intergenic | 20.0% |
| !!! | AAAATGATTTGCTTAAAGAT+AGG | + | chr4.1:47678764-47678783 | MS.gene61626:CDS | 20.0% |
| !!! | AAATGATTTGCTTAAAGATA+GGG | + | chr4.1:47678765-47678784 | MS.gene61626:CDS | 20.0% |
| ! | ATAGAGTATGTAGAATTTGA+AGG | + | chr4.1:47678829-47678848 | MS.gene61626:CDS | 25.0% |
| ! | CAATACCTGTTCAATATTAA+AGG | - | chr4.1:47678628-47678647 | None:intergenic | 25.0% |
| ! | CTTTAATATTGAACAGGTAT+TGG | + | chr4.1:47678626-47678645 | MS.gene61626:intron | 25.0% |
| ! | TAATATAGCTCATAATTTGG+CGG | + | chr4.1:47678061-47678080 | MS.gene61626:CDS | 25.0% |
| ! | TGGTAATATAGCTCATAATT+TGG | + | chr4.1:47678058-47678077 | MS.gene61626:CDS | 25.0% |
| ! | TTGTACCTTTAATATTGAAC+AGG | + | chr4.1:47678620-47678639 | MS.gene61626:intron | 25.0% |
| !! | GGTATTTGTTACTTATTGAA+AGG | + | chr4.1:47678586-47678605 | MS.gene61626:intron | 25.0% |
| !!! | CACTTTTTATATTGTTCTCA+CGG | - | chr4.1:47678534-47678553 | None:intergenic | 25.0% |
| !!! | TTTTAACCGGGTTTTTATTT+CGG | + | chr4.1:47678022-47678041 | MS.gene61626:CDS | 25.0% |
| AATTAGACGTAATCATGTGT+CGG | + | chr4.1:47678381-47678400 | MS.gene61626:intron | 30.0% | |
| AATTTGAAGGACAACAACAT+GGG | + | chr4.1:47678842-47678861 | MS.gene61626:CDS | 30.0% | |
| TGAGAAAAGAGTTACAAACT+CGG | + | chr4.1:47678309-47678328 | MS.gene61626:intron | 30.0% | |
| ! | CCAATCTTTTACTACATTCA+CGG | + | chr4.1:47677780-47677799 | MS.gene61626:CDS | 30.0% |
| !! | GTAAAAGATTGGTAGCTTTT+TGG | - | chr4.1:47677772-47677791 | None:intergenic | 30.0% |
| !! | TTTAACCGGGTTTTTATTTC+GGG | + | chr4.1:47678023-47678042 | MS.gene61626:CDS | 30.0% |
| !!! | TCTTTTGATGGTTCTGAATT+TGG | - | chr4.1:47678883-47678902 | None:intergenic | 30.0% |
| ATGCAAAAAGGCTCAAGAAT+TGG | + | chr4.1:47678797-47678816 | MS.gene61626:CDS | 35.0% | |
| CCGTGAATGTAGTAAAAGAT+TGG | - | chr4.1:47677783-47677802 | None:intergenic | 35.0% | |
| GAATTTGAAGGACAACAACA+TGG | + | chr4.1:47678841-47678860 | MS.gene61626:CDS | 35.0% | |
| GTTCTTCATGTCTATAACGA+CGG | + | chr4.1:47677609-47677628 | MS.gene61626:CDS | 35.0% | |
| TCAACTTGGAACTTATTGAC+AGG | + | chr4.1:47678210-47678229 | MS.gene61626:CDS | 35.0% | |
| TGCAAAAAGGCTCAAGAATT+GGG | + | chr4.1:47678798-47678817 | MS.gene61626:CDS | 35.0% | |
| TGTTTACATATCTGACATCG+AGG | + | chr4.1:47678409-47678428 | MS.gene61626:intron | 35.0% | |
| ! | AAGACAACGACACTGTTTTA+TGG | + | chr4.1:47677676-47677695 | MS.gene61626:CDS | 35.0% |
| ! | ACCAACATCAACTCTTTTGA+TGG | - | chr4.1:47678895-47678914 | None:intergenic | 35.0% |
| ! | ATCTTTTACTACATTCACGG+TGG | + | chr4.1:47677783-47677802 | MS.gene61626:CDS | 35.0% |
| ! | GTGTGTCTAGAATAGAACAT+TGG | + | chr4.1:47678565-47678584 | MS.gene61626:intron | 35.0% |
| !! | AAAGATTGGTAGCTTTTTGG+AGG | - | chr4.1:47677769-47677788 | None:intergenic | 35.0% |
| !! | AAGATTGGTAGCTTTTTGGA+GGG | - | chr4.1:47677768-47677787 | None:intergenic | 35.0% |
| !! | AAGTTGAGAAAAGCATCATC+CGG | - | chr4.1:47678197-47678216 | None:intergenic | 35.0% |
| !! | ACCATCAAAAGAGTTGATGT+TGG | + | chr4.1:47678891-47678910 | MS.gene61626:CDS | 35.0% |
| !!! | AAGCAGTAGTTTTGACAGTT+TGG | - | chr4.1:47677837-47677856 | None:intergenic | 35.0% |
| AAACCGTCTTCAATAGCACA+TGG | - | chr4.1:47677930-47677949 | None:intergenic | 40.0% | |
| ACTATAGTTGACGAATGTCG+TGG | + | chr4.1:47677585-47677604 | MS.gene61626:CDS | 40.0% | |
| AGTCACCCGAAATAAAAACC+CGG | - | chr4.1:47678031-47678050 | None:intergenic | 40.0% | |
| ATGATCAGTATTCTCTCCGA+TGG | - | chr4.1:47678665-47678684 | None:intergenic | 40.0% | |
| CATCCACTTGTGAATCCATT+TGG | + | chr4.1:47678682-47678701 | MS.gene61626:CDS | 40.0% | |
| CTCTTACTTCTTGGTCCAAA+TGG | - | chr4.1:47678700-47678719 | None:intergenic | 40.0% | |
| GCAAAAAGGCTCAAGAATTG+GGG | + | chr4.1:47678799-47678818 | MS.gene61626:CDS | 40.0% | |
| GGTCAAGATCAATTTCCTCA+AGG | - | chr4.1:47678723-47678742 | None:intergenic | 40.0% | |
| GTCTTGAACTGTGATGTTGA+AGG | - | chr4.1:47677661-47677680 | None:intergenic | 40.0% | |
| TAGCTCATAATTTGGCGGTT+CGG | + | chr4.1:47678066-47678085 | MS.gene61626:CDS | 40.0% | |
| TGATCAGTATTCTCTCCGAT+GGG | - | chr4.1:47678664-47678683 | None:intergenic | 40.0% | |
| TGATCTTGACCCAATTCTTG+TGG | + | chr4.1:47678732-47678751 | MS.gene61626:CDS | 40.0% | |
| TGCTATTGAAGACGGTTTCA+TGG | + | chr4.1:47677932-47677951 | MS.gene61626:CDS | 40.0% | |
| TGGAGACTTTCTATACCCAT+CGG | + | chr4.1:47678646-47678665 | MS.gene61626:CDS | 40.0% | |
| TTTGCTTAAAGATAGGGCAG+AGG | + | chr4.1:47678771-47678790 | MS.gene61626:CDS | 40.0% | |
| ! | ATGAGTTAGCTGGCTTATAG+AGG | - | chr4.1:47677743-47677762 | None:intergenic | 40.0% |
| ! | CTTCCATGTGCTATTGAAGA+CGG | + | chr4.1:47677924-47677943 | MS.gene61626:CDS | 40.0% |
| ! | GGATGATGCTTTTCTCAACT+TGG | + | chr4.1:47678196-47678215 | MS.gene61626:CDS | 40.0% |
| ! | GGTTAGGGGTTATGTTTTGT+TGG | + | chr4.1:47678124-47678143 | MS.gene61626:CDS | 40.0% |
| ! | TTTTACTACATTCACGGTGG+CGG | + | chr4.1:47677786-47677805 | MS.gene61626:CDS | 40.0% |
| !! | AGAAGCTTTGAAGAGTGAGA+TGG | + | chr4.1:47677974-47677993 | MS.gene61626:CDS | 40.0% |
| !! | AGATTGGTAGCTTTTTGGAG+GGG | - | chr4.1:47677767-47677786 | None:intergenic | 40.0% |
| !! | ATAATTTGGCGGTTCGGTTT+GGG | + | chr4.1:47678072-47678091 | MS.gene61626:CDS | 40.0% |
| !! | GGTTTTTATTTCGGGTGACT+CGG | + | chr4.1:47678031-47678050 | MS.gene61626:CDS | 40.0% |
| !! | GTTTTGTTGGCACCGTTTTT+TGG | + | chr4.1:47678137-47678156 | MS.gene61626:CDS | 40.0% |
| ACCAAGAAGTAAGAGCCTTG+AGG | + | chr4.1:47678705-47678724 | MS.gene61626:CDS | 45.0% | |
| ACGGTTTCATGGCTGTTAAG+TGG | + | chr4.1:47677943-47677962 | MS.gene61626:CDS | 45.0% | |
| CTCACGGTTCCACCAAAAAA+CGG | - | chr4.1:47678152-47678171 | None:intergenic | 45.0% | |
| CTCCAACCACCACAAGAATT+GGG | - | chr4.1:47678744-47678763 | None:intergenic | 45.0% | |
| GAACTGTGATGTTGAAGGCT+GGG | - | chr4.1:47677656-47677675 | None:intergenic | 45.0% | |
| GGGCAGAGGATTATGCAAAA+AGG | + | chr4.1:47678785-47678804 | MS.gene61626:CDS | 45.0% | |
| TCCTCAAGGCTCTTACTTCT+TGG | - | chr4.1:47678709-47678728 | None:intergenic | 45.0% | |
| TCTCCAACCACCACAAGAAT+TGG | - | chr4.1:47678745-47678764 | None:intergenic | 45.0% | |
| TCTTGACCCAATTCTTGTGG+TGG | + | chr4.1:47678735-47678754 | MS.gene61626:CDS | 45.0% | |
| TGAACTGTGATGTTGAAGGC+TGG | - | chr4.1:47677657-47677676 | None:intergenic | 45.0% | |
| TGAAGAGTGAGATGGATCCT+TGG | + | chr4.1:47677982-47678001 | MS.gene61626:CDS | 45.0% | |
| TGGGTTTAACGTGCGAACTA+TGG | - | chr4.1:47677637-47677656 | None:intergenic | 45.0% | |
| ! | GGTCCAAATGGATTCACAAG+TGG | - | chr4.1:47678688-47678707 | None:intergenic | 45.0% |
| !! | CATAATTTGGCGGTTCGGTT+TGG | + | chr4.1:47678071-47678090 | MS.gene61626:CDS | 45.0% |
| !! | CCATGTGGCTGATTTTAACC+GGG | + | chr4.1:47678010-47678029 | MS.gene61626:CDS | 45.0% |
| !! | GATTGGTAGCTTTTTGGAGG+GGG | - | chr4.1:47677766-47677785 | None:intergenic | 45.0% |
| !! | GCCATGTGGCTGATTTTAAC+CGG | + | chr4.1:47678009-47678028 | MS.gene61626:CDS | 45.0% |
| !!! | TTGTTGGCACCGTTTTTTGG+TGG | + | chr4.1:47678140-47678159 | MS.gene61626:CDS | 45.0% |
| ACATAACCCCTAACCCGAAC+CGG | - | chr4.1:47678119-47678138 | None:intergenic | 50.0% | |
| AGGACCAAGTCAGAAGCTGA+AGG | + | chr4.1:47678170-47678189 | MS.gene61626:CDS | 50.0% | |
| CAAGTCAGAAGCTGAAGGAC+CGG | + | chr4.1:47678175-47678194 | MS.gene61626:CDS | 50.0% | |
| CCCGGTTAAAATCAGCCACA+TGG | - | chr4.1:47678013-47678032 | None:intergenic | 50.0% | |
| GACCCAATTCTTGTGGTGGT+TGG | + | chr4.1:47678739-47678758 | MS.gene61626:CDS | 50.0% | |
| GGAAACGAACACTAGACACG+CGG | + | chr4.1:47678330-47678349 | MS.gene61626:intron | 50.0% | |
| ! | TAGCTGGCTTATAGAGGCGA+AGG | - | chr4.1:47677737-47677756 | None:intergenic | 50.0% |
| ! | TTTATTTCGGGTGACTCGGC+TGG | + | chr4.1:47678035-47678054 | MS.gene61626:CDS | 50.0% |
| !! | AGCTTCTGACTTGGTCCTCA+CGG | - | chr4.1:47678168-47678187 | None:intergenic | 50.0% |
| !! | ATCGACTTGCGCCAGAGAAT+CGG | + | chr4.1:47677901-47677920 | MS.gene61626:CDS | 50.0% |
| !! | GGTTCGGTTTGGGTTTGGTT+CGG | + | chr4.1:47678082-47678101 | MS.gene61626:CDS | 50.0% |
| !! | TGGGTTTGGTTCGGTTGAGT+TGG | + | chr4.1:47678091-47678110 | MS.gene61626:CDS | 50.0% |
| !!! | CGTTTTTTGGTGGAACCGTG+AGG | + | chr4.1:47678150-47678169 | MS.gene61626:CDS | 50.0% |
| CGGTCCTTCAGCTTCTGACT+TGG | - | chr4.1:47678177-47678196 | None:intergenic | 55.0% | |
| GGATCCTTGGTTGAGCCATG+TGG | + | chr4.1:47677995-47678014 | MS.gene61626:CDS | 55.0% | |
| TCAGCCACATGGCTCAACCA+AGG | - | chr4.1:47678002-47678021 | None:intergenic | 55.0% | |
| TCTGGCGCAAGTCGATAGTC+AGG | - | chr4.1:47677897-47677916 | None:intergenic | 55.0% | |
| TTGGAGGGGGATGAGTTAGC+TGG | - | chr4.1:47677753-47677772 | None:intergenic | 55.0% | |
| ! | GCACATGGAAGCCGATTCTC+TGG | - | chr4.1:47677915-47677934 | None:intergenic | 55.0% |
| !! | TCTGCATCGGCTCACGAACA+TGG | + | chr4.1:47677811-47677830 | MS.gene61626:CDS | 55.0% |
| !! | TGGTTCGGTTGAGTTGGCAC+CGG | + | chr4.1:47678097-47678116 | MS.gene61626:CDS | 55.0% |
| !! | TTGGCGGTTCGGTTTGGGTT+TGG | + | chr4.1:47678077-47678096 | MS.gene61626:CDS | 55.0% |
| ! | ATTTCGGGTGACTCGGCTGG+TGG | + | chr4.1:47678038-47678057 | MS.gene61626:CDS | 60.0% |
| !! | AGTTGGCACCGGTTCGGGTT+AGG | + | chr4.1:47678108-47678127 | MS.gene61626:CDS | 60.0% |
| !! | CGGTTGAGTTGGCACCGGTT+CGG | + | chr4.1:47678102-47678121 | MS.gene61626:CDS | 60.0% |
| !! | GGTTGAGTTGGCACCGGTTC+GGG | + | chr4.1:47678103-47678122 | MS.gene61626:CDS | 60.0% |
| !! | GTTGGCACCGGTTCGGGTTA+GGG | + | chr4.1:47678109-47678128 | MS.gene61626:CDS | 60.0% |
| !! | TTGGCACCGGTTCGGGTTAG+GGG | + | chr4.1:47678110-47678129 | MS.gene61626:CDS | 60.0% |
| ! | CACGGTGGCGGCTTCTGCAT+CGG | + | chr4.1:47677798-47677817 | MS.gene61626:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 47677576 | 47678942 | 47677576 | ID=MS.gene61626 |
| chr4.1 | mRNA | 47677576 | 47678942 | 47677576 | ID=MS.gene61626.t1;Parent=MS.gene61626 |
| chr4.1 | exon | 47677576 | 47678231 | 47677576 | ID=MS.gene61626.t1.exon1;Parent=MS.gene61626.t1 |
| chr4.1 | CDS | 47677576 | 47678231 | 47677576 | ID=cds.MS.gene61626.t1;Parent=MS.gene61626.t1 |
| chr4.1 | exon | 47678642 | 47678942 | 47678642 | ID=MS.gene61626.t1.exon2;Parent=MS.gene61626.t1 |
| chr4.1 | CDS | 47678642 | 47678942 | 47678642 | ID=cds.MS.gene61626.t1;Parent=MS.gene61626.t1 |
| Gene Sequence |
| Protein sequence |
