AlfalfaGEDB | MS.gene61679

M. sativa cultivar XinJiangDaYe / MS.gene61679

KEGG: K11251
GO: GO:0000786 GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0005515 GO:0005575 GO:0005634 GO:0032991 GO:0032993 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044815 GO:0046982 GO:0046983 GO:0097159 GO:0110165 GO:1901363
PFAM: PF00125.27 PF16211.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61679.t1	XP_004498649.1	98	100	2	0	14	113	35	134	1.00E-45	192.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61679.t1	Q8H7Y8	94.0	100	6	0	14	113	39	138	5.0e-46	184.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61679.t1	G7I6D5	98.0	100	2	0	14	113	35	134	7.2e-46	192.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049255	MS.gene61679	0.818037	2.42E-52	-1.69E-46
MS.gene050277	MS.gene61679	0.844107	9.28E-59	-1.69E-46
MS.gene050303	MS.gene61679	0.822868	1.88E-53	-1.69E-46
MS.gene050916	MS.gene61679	0.835361	1.75E-56	-1.69E-46
MS.gene051241	MS.gene61679	0.806434	8.24E-50	-1.69E-46
MS.gene051330	MS.gene61679	0.814236	1.71E-51	-1.69E-46
MS.gene05134	MS.gene61679	0.810195	1.30E-50	-1.69E-46
MS.gene051903	MS.gene61679	0.841041	6.04E-58	-1.69E-46
MS.gene052947	MS.gene61679	0.849642	2.84E-60	-1.69E-46
MS.gene053294	MS.gene61679	0.80456	2.04E-49	-1.69E-46
MS.gene055555	MS.gene61679	0.817564	3.09E-52	-1.69E-46
MS.gene055808	MS.gene61679	0.841194	5.51E-58	-1.69E-46
MS.gene056075	MS.gene61679	0.808686	2.74E-50	-1.69E-46
MS.gene056091	MS.gene61679	0.815285	1.00E-51	-1.69E-46
MS.gene056601	MS.gene61679	0.81295	3.28E-51	-1.69E-46
MS.gene056631	MS.gene61679	0.85332	2.58E-61	-1.69E-46
MS.gene057206	MS.gene61679	0.800293	1.54E-48	-1.69E-46
MS.gene057705	MS.gene61679	0.859388	4.28E-63	-1.69E-46
MS.gene058238	MS.gene61679	0.806301	8.79E-50	-1.69E-46
MS.gene06001	MS.gene61679	0.850052	2.18E-60	-1.69E-46
MS.gene060429	MS.gene61679	0.837355	5.46E-57	-1.69E-46
MS.gene060917	MS.gene61679	0.806022	1.01E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61679.t1	MTR_1g011750	98.000	100	2	0	14	113	35	134	1.26e-66	197
MS.gene61679.t1	MTR_1g011850	98.000	100	2	0	14	113	35	134	1.26e-66	197
MS.gene61679.t1	MTR_1g071040	90.000	100	10	0	14	113	35	134	1.65e-60	181
MS.gene61679.t1	MTR_1g114050	86.000	100	14	0	14	113	32	131	2.92e-57	173
MS.gene61679.t1	MTR_4g048490	83.582	67	11	0	47	113	39	105	9.70e-35	115
MS.gene61679.t1	MTR_7g108320	60.825	97	36	2	14	109	31	126	2.10e-33	113
MS.gene61679.t1	MTR_7g114060	60.825	97	36	2	14	109	28	123	3.83e-33	112
MS.gene61679.t1	MTR_7g114040	60.825	97	36	2	14	109	28	123	3.83e-33	112
MS.gene61679.t1	MTR_2g082220	60.606	99	37	2	14	111	25	122	1.27e-32	111
MS.gene61679.t1	MTR_2g082510	60.606	99	37	2	14	111	25	122	1.34e-32	111
MS.gene61679.t1	MTR_7g093910	58.763	97	38	2	14	109	31	126	1.12e-31	109
MS.gene61679.t1	MTR_8g086640	58.416	101	40	2	14	113	33	132	4.59e-30	105
MS.gene61679.t1	MTR_4g071150	59.000	100	39	2	14	112	33	131	8.26e-30	104
MS.gene61679.t1	MTR_7g113160	54.545	99	43	2	14	111	35	132	8.99e-30	104
MS.gene61679.t1	MTR_4g064967	57.426	101	41	2	14	113	33	132	1.28e-29	103
MS.gene61679.t1	MTR_4g064005	57.426	101	41	2	14	113	33	132	1.35e-29	103
MS.gene61679.t1	MTR_2g096570	58.000	100	40	2	14	112	32	130	1.46e-29	103
MS.gene61679.t1	MTR_4g063280	57.426	101	41	2	14	113	27	126	1.58e-29	103
MS.gene61679.t1	MTR_2g096610	57.426	101	41	2	14	113	31	130	1.77e-29	103
MS.gene61679.t1	MTR_4g063410	58.763	97	38	2	14	109	33	128	8.11e-29	102
MS.gene61679.t1	MTR_7g027610	55.000	100	21	1	14	113	23	98	1.04e-25	93.2
MS.gene61679.t1	MTR_0653s0030	87.500	48	6	0	44	91	50	97	1.30e-22	86.7
MS.gene61679.t1	MTR_7g114050	51.515	99	35	4	14	111	30	116	3.52e-22	84.7
MS.gene61679.t1	MTR_4g063300	62.687	67	24	1	34	99	17	83	1.16e-19	77.8
MS.gene61679.t1	MTR_6g005150	42.000	100	47	4	14	111	35	125	1.23e-14	65.5
MS.gene61679.t1	MTR_7g427690	78.947	38	8	0	75	112	412	449	2.35e-12	62.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61679.t1	AT1G52740	91.000	100	9	0	14	113	35	134	9.44e-63	187
MS.gene61679.t1	AT3G54560	91.000	100	9	0	14	113	37	136	6.86e-62	185
MS.gene61679.t1	AT3G54560	91.000	100	9	0	14	113	37	136	6.86e-62	185
MS.gene61679.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene61679.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene61679.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene61679.t1	AT2G38810	89.000	100	11	0	14	113	37	136	1.37e-60	182
MS.gene61679.t1	AT4G13570	72.165	97	27	0	7	103	22	118	1.72e-44	140
MS.gene61679.t1	AT4G13570	76.667	90	21	0	14	103	35	124	5.52e-44	139
MS.gene61679.t1	AT1G08880	62.105	95	34	2	14	107	31	124	2.51e-33	113
MS.gene61679.t1	AT1G54690	61.053	95	35	2	14	107	31	124	7.88e-33	112
MS.gene61679.t1	AT4G27230	60.606	99	37	2	14	111	25	122	1.25e-32	111
MS.gene61679.t1	AT4G27230	60.606	99	37	2	14	111	25	122	1.25e-32	111
MS.gene61679.t1	AT5G54640	60.606	99	37	2	14	111	25	122	1.38e-32	111
MS.gene61679.t1	AT1G51060	60.606	99	37	2	14	111	25	122	1.88e-32	110
MS.gene61679.t1	AT3G20670	60.606	99	37	2	14	111	25	122	3.15e-32	110
MS.gene61679.t1	AT5G02560	59.406	101	39	2	14	113	33	132	4.86e-31	107
MS.gene61679.t1	AT5G59870	60.606	99	37	2	14	111	33	130	5.33e-31	107
MS.gene61679.t1	AT5G27670	60.606	99	37	2	14	111	34	131	6.10e-30	105
MS.gene61679.t1	AT5G02560	48.000	125	39	3	14	113	33	156	1.10e-25	95.1

Find 32 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TATGAACATTCATTCCTTTG+AGG	0.376752	1.3:-9281781	None:intergenic
CCTCTGCAGTTAAATATTCC+AGG	0.413090	1.3:-9281590	None:intergenic
CAATGGCACATGGAAGAGTT+GGG	0.442531	1.3:+9281538	MS.gene61679:CDS
AGTTTATTCTGCCGCTATCC+TGG	0.454001	1.3:+9281572	MS.gene61679:CDS
ACAATGGCACATGGAAGAGT+TGG	0.476378	1.3:+9281537	MS.gene61679:CDS
TGCTTCAGCCACATGTGTCA+TGG	0.477968	1.3:+9281219	None:intergenic
ATATTTAACTGCAGAGGTGT+TGG	0.485770	1.3:+9281596	MS.gene61679:CDS
CTCATCAAAGGAACAATAGC+TGG	0.487626	1.3:+9281714	MS.gene61679:CDS
ATAAGAGACTTGTGGATATG+AGG	0.507095	1.3:-9281750	None:intergenic
AGACGATGGATACGTCCGAC+AGG	0.539450	1.3:-9281501	None:intergenic
AACTGCAGAGGTGTTGGAGT+TGG	0.540859	1.3:+9281602	MS.gene61679:CDS
GCAGAGGTGTTGGAGTTGGC+TGG	0.558822	1.3:+9281606	MS.gene61679:CDS
CAATGCAAGCAAGGATCTGA+AGG	0.565081	1.3:+9281629	MS.gene61679:CDS
GTTGGCTGGCAATGCAAGCA+AGG	0.571379	1.3:+9281620	MS.gene61679:CDS
GAACTTGACACTCTCATCAA+AGG	0.571379	1.3:+9281702	MS.gene61679:CDS
ACTTGTTGATAAGAGACTTG+TGG	0.572936	1.3:-9281758	None:intergenic
ACATGTGTCATGGCTACAGC+AGG	0.574358	1.3:+9281229	None:intergenic
TGTCAAGTTCTTCGTCTCCA+CGG	0.583078	1.3:-9281689	None:intergenic
ATAGCAAGCTGTAGATGTCT+CGG	0.584334	1.3:-9281666	None:intergenic
TCTTATCAACAAGTCCTCAA+AGG	0.584732	1.3:+9281767	MS.gene61679:CDS
CATCTACAGCTTGCTATCCG+TGG	0.586408	1.3:+9281672	MS.gene61679:CDS
TCCATCGTCTTCTGAAGTCA+AGG	0.589423	1.3:+9281514	MS.gene61679:CDS
AAAGGAACAATAGCTGGTGG+TGG	0.608922	1.3:+9281720	MS.gene61679:CDS
CCTGGAATATTTAACTGCAG+AGG	0.615265	1.3:+9281590	MS.gene61679:CDS
AAGTCAAGGACAATGGCACA+TGG	0.622887	1.3:+9281528	MS.gene61679:CDS
GACGATGGATACGTCCGACA+GGG	0.626692	1.3:-9281500	None:intergenic
TTCTACATACAGTTCCCTGT+CGG	0.628096	1.3:+9281486	MS.gene61679:intron
AATGGCACATGGAAGAGTTG+GGG	0.645846	1.3:+9281539	MS.gene61679:CDS
TCTTCTGAAGTCAAGGACAA+TGG	0.655563	1.3:+9281521	MS.gene61679:CDS
ATCAAAGGAACAATAGCTGG+TGG	0.661257	1.3:+9281717	MS.gene61679:CDS
TCCTTGACTTCAGAAGACGA+TGG	0.668973	1.3:-9281515	None:intergenic
AGTTAAATATTCCAGGATAG+CGG	0.686777	1.3:-9281583	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GTAAATTATGTTGCATTTGT+TGG	+	chr1.3:9281411-9281430	MS.gene61679:intron	25.0%
	AATAATTAACCGCTTGCAAA+AGG	-	chr1.3:9281320-9281339	None:intergenic	30.0%
	AGTTAAATATTCCAGGATAG+CGG	-	chr1.3:9281586-9281605	None:intergenic	30.0%
	ATGTAGAAAGATGTTCATGA+TGG	-	chr1.3:9281474-9281493	None:intergenic	30.0%
!	TGATTATTTCCTTTTGCAAG+CGG	+	chr1.3:9281308-9281327	MS.gene61679:intron	30.0%
!!	ATTTTAGCATGCTTAGAATG+TGG	-	chr1.3:9281378-9281397	None:intergenic	30.0%
	ATAAGAGACTTGTGGATATG+AGG	-	chr1.3:9281753-9281772	None:intergenic	35.0%
	ATTTATCCCACATCATCATC+CGG	+	chr1.3:9281444-9281463	MS.gene61679:intron	35.0%
	TCTTATCAACAAGTCCTCAA+AGG	+	chr1.3:9281767-9281786	MS.gene61679:CDS	35.0%
!	ACTTGTTGATAAGAGACTTG+TGG	-	chr1.3:9281761-9281780	None:intergenic	35.0%
!	AGATGTTCATGATGGAAAAC+CGG	-	chr1.3:9281466-9281485	None:intergenic	35.0%
!	ATATTTAACTGCAGAGGTGT+TGG	+	chr1.3:9281596-9281615	MS.gene61679:CDS	35.0%
!!	GCATGCTAAAATTTGTTGTC+TGG	+	chr1.3:9281386-9281405	MS.gene61679:intron	35.0%
	ATAGCAAGCTGTAGATGTCT+CGG	-	chr1.3:9281669-9281688	None:intergenic	40.0%
	ATCAAAGGAACAATAGCTGG+TGG	+	chr1.3:9281717-9281736	MS.gene61679:CDS	40.0%
	CCTCTGCAGTTAAATATTCC+AGG	-	chr1.3:9281593-9281612	None:intergenic	40.0%
	CCTGGAATATTTAACTGCAG+AGG	+	chr1.3:9281590-9281609	MS.gene61679:CDS	40.0%
	CTCATCAAAGGAACAATAGC+TGG	+	chr1.3:9281714-9281733	MS.gene61679:CDS	40.0%
	CTGTGAGTGACTAGTATCTT+TGG	+	chr1.3:9281274-9281293	MS.gene61679:intron	40.0%
	GAACTTGACACTCTCATCAA+AGG	+	chr1.3:9281702-9281721	MS.gene61679:CDS	40.0%
	TTCTACATACAGTTCCCTGT+CGG	+	chr1.3:9281486-9281505	MS.gene61679:intron	40.0%
!!	TCTTCTGAAGTCAAGGACAA+TGG	+	chr1.3:9281521-9281540	MS.gene61679:CDS	40.0%
	AAAGGAACAATAGCTGGTGG+TGG	+	chr1.3:9281720-9281739	MS.gene61679:CDS	45.0%
	AAGTCAAGGACAATGGCACA+TGG	+	chr1.3:9281528-9281547	MS.gene61679:CDS	45.0%
	AATGGCACATGGAAGAGTTG+GGG	+	chr1.3:9281539-9281558	MS.gene61679:CDS	45.0%
	ACAATGGCACATGGAAGAGT+TGG	+	chr1.3:9281537-9281556	MS.gene61679:CDS	45.0%
	AGTTTATTCTGCCGCTATCC+TGG	+	chr1.3:9281572-9281591	MS.gene61679:CDS	45.0%
	CAATGCAAGCAAGGATCTGA+AGG	+	chr1.3:9281629-9281648	MS.gene61679:CDS	45.0%
	CAATGGCACATGGAAGAGTT+GGG	+	chr1.3:9281538-9281557	MS.gene61679:CDS	45.0%
	TCCTTGACTTCAGAAGACGA+TGG	-	chr1.3:9281518-9281537	None:intergenic	45.0%
	TGTCAAGTTCTTCGTCTCCA+CGG	-	chr1.3:9281692-9281711	None:intergenic	45.0%
!	GGAAAACCGGATGATGATGT+GGG	-	chr1.3:9281453-9281472	None:intergenic	45.0%
!	TGGAAAACCGGATGATGATG+TGG	-	chr1.3:9281454-9281473	None:intergenic	45.0%
!!	TCCATCGTCTTCTGAAGTCA+AGG	+	chr1.3:9281514-9281533	MS.gene61679:CDS	45.0%
!!	AACTGCAGAGGTGTTGGAGT+TGG	+	chr1.3:9281602-9281621	MS.gene61679:CDS	50.0%
!!	CATCTACAGCTTGCTATCCG+TGG	+	chr1.3:9281672-9281691	MS.gene61679:CDS	50.0%
	AGACGATGGATACGTCCGAC+AGG	-	chr1.3:9281504-9281523	None:intergenic	55.0%
	GACGATGGATACGTCCGACA+GGG	-	chr1.3:9281503-9281522	None:intergenic	55.0%
	GTTGGCTGGCAATGCAAGCA+AGG	+	chr1.3:9281620-9281639	MS.gene61679:CDS	55.0%
!!	GCAGAGGTGTTGGAGTTGGC+TGG	+	chr1.3:9281606-9281625	MS.gene61679:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.3	gene	9281231	9281794	9281231	ID=MS.gene61679
chr1.3	mRNA	9281231	9281794	9281231	ID=MS.gene61679.t1;Parent=MS.gene61679
chr1.3	exon	9281231	9281275	9281231	ID=MS.gene61679.t1.exon1;Parent=MS.gene61679.t1
chr1.3	CDS	9281231	9281275	9281231	ID=cds.MS.gene61679.t1;Parent=MS.gene61679.t1
chr1.3	exon	9281498	9281794	9281498	ID=MS.gene61679.t1.exon2;Parent=MS.gene61679.t1
chr1.3	CDS	9281498	9281794	9281498	ID=cds.MS.gene61679.t1;Parent=MS.gene61679.t1

Gene Sequence

>MS.gene61679

ATGTGTCATGGCTACAGCAGGTTCATCTATAACAATGATATTGCTGTGAGTGACTAGTATCTTTGGATGCTGTGTGTTGATTATTTCCTTTTGCAAGCGGTTAATTATTGTATCTCTTTGATTCTTGAAACTATTTTTTTTCTTCCACATTCTAAGCATGCTAAAATTTGTTGTCTGGCTGTAAATTATGTTGCATTTGTTGGTATTGTTTAAATTTATCCCACATCATCATCCGGTTTTCCATCATGAACATCTTTCTACATACAGTTCCCTGTCGGACGTATCCATCGTCTTCTGAAGTCAAGGACAATGGCACATGGAAGAGTTGGGGCTACAGCTGCAGTTTATTCTGCCGCTATCCTGGAATATTTAACTGCAGAGGTGTTGGAGTTGGCTGGCAATGCAAGCAAGGATCTGAAGGTGAAGCGTATTACACCGAGACATCTACAGCTTGCTATCCGTGGAGACGAAGAACTTGACACTCTCATCAAAGGAACAATAGCTGGTGGTGGTGTTATTCCTCATATCCACAAGTCTCTTATCAACAAGTCCTCAAAGGAATGA

Protein sequence

>MS.gene61679.t1

MCHGYSRFIYNNDIAFPVGRIHRLLKSRTMAHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDTLIKGTIAGGGVIPHIHKSLINKSSKE