AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62674

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62674.t1 MTR_8g105740 97.890 853 18 0 1 853 1 853 0.0 1736
MS.gene62674.t1 MTR_1g032730 86.114 857 111 6 1 852 1 854 0.0 1511
MS.gene62674.t1 MTR_1g109620 73.059 850 221 5 1 849 1 843 0.0 1308
MS.gene62674.t1 MTR_4g080160 63.594 857 295 7 1 848 1 849 0.0 1150
MS.gene62674.t1 MTR_8g063790 64.906 852 283 7 1 849 1 839 0.0 1129
MS.gene62674.t1 MTR_4g129270 58.416 808 321 8 47 849 43 840 0.0 994
MS.gene62674.t1 MTR_4g129270 58.971 719 282 7 47 760 43 753 0.0 894
MS.gene62674.t1 MTR_3g078210 54.642 754 335 5 87 837 3 752 0.0 819
MS.gene62674.t1 MTR_3g467030 49.261 609 274 6 62 670 1 574 0.0 555
MS.gene62674.t1 MTR_2g073260 35.263 760 427 20 59 799 122 835 2.89e-141 443
MS.gene62674.t1 MTR_0034s0170 35.363 772 431 25 59 809 83 807 2.11e-136 429
MS.gene62674.t1 MTR_8g087910 38.623 334 178 10 58 387 74 384 2.88e-68 232
MS.gene62674.t1 MTR_8g087930 31.498 454 260 15 394 828 10 431 2.37e-53 194
MS.gene62674.t1 MTR_3g076810 51.095 137 62 3 61 197 39 170 7.09e-39 142
MS.gene62674.t1 MTR_3g467040 44.828 145 71 2 457 601 87 222 1.61e-33 129
MS.gene62674.t1 MTR_3g467040 44.144 111 60 1 313 423 1 109 1.13e-20 92.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62674.t1 AT1G68020 80.000 865 152 4 1 851 1 858 0.0 1441
MS.gene62674.t1 AT1G68020 80.000 865 152 4 1 851 1 858 0.0 1441
MS.gene62674.t1 AT4G17770 71.161 853 236 5 1 850 1 846 0.0 1282
MS.gene62674.t1 AT4G17770 71.161 853 236 5 1 850 1 846 0.0 1282
MS.gene62674.t1 AT1G68020 80.470 681 114 3 1 669 1 674 0.0 1142
MS.gene62674.t1 AT1G23870 63.647 850 299 6 1 847 1 843 0.0 1123
MS.gene62674.t1 AT1G06410 63.435 856 287 8 1 848 1 838 0.0 1121
MS.gene62674.t1 AT1G06410 63.435 856 287 8 1 848 1 838 0.0 1121
MS.gene62674.t1 AT1G06410 63.435 856 287 8 1 848 1 838 0.0 1121
MS.gene62674.t1 AT1G70290 63.002 846 300 6 1 844 1 835 0.0 1112
MS.gene62674.t1 AT1G60140 62.471 850 309 6 1 847 1 843 0.0 1104
MS.gene62674.t1 AT1G60140 62.471 850 309 6 1 847 1 843 0.0 1104
MS.gene62674.t1 AT1G60140 62.471 850 309 6 1 847 1 843 0.0 1104
MS.gene62674.t1 AT1G60140 62.471 850 309 6 1 847 1 843 0.0 1104
MS.gene62674.t1 AT1G60140 62.471 850 309 6 1 847 1 843 0.0 1104
MS.gene62674.t1 AT2G18700 58.734 853 330 9 1 848 1 836 0.0 1039
MS.gene62674.t1 AT1G70290 58.983 846 296 7 1 844 1 797 0.0 1020
MS.gene62674.t1 AT1G60140 62.812 761 275 5 1 760 1 754 0.0 995
MS.gene62674.t1 AT1G16980 34.601 815 461 25 58 848 10 776 6.52e-145 448
MS.gene62674.t1 AT1G78580 34.777 785 441 23 49 810 81 817 4.97e-139 436
MS.gene62674.t1 AT1G78580 34.777 785 441 23 49 810 81 817 4.97e-139 436
MS.gene62674.t1 AT1G78580 34.777 785 441 23 49 810 81 817 4.97e-139 436
MS.gene62674.t1 AT1G78580 34.777 785 441 23 49 810 81 817 4.97e-139 436
MS.gene62674.t1 AT4G27550 32.446 826 469 22 59 843 3 780 1.13e-131 412
MS.gene62674.t1 AT4G27550 32.569 829 473 24 59 843 3 789 3.16e-130 409
MS.gene62674.t1 AT1G16980 35.591 635 350 18 58 681 10 596 5.01e-119 375

Find 199 sgRNAs with CRISPR-Local

Find 278 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACAAGACATTGCCTATT+AGG 0.162279 4.2:+1244773 MS.gene62674:CDS
CAAGTAGTACTTGGCCTTGC+TGG 0.213909 4.2:-1247489 None:intergenic
GAGGATGCAGATCCTGATTT+TGG 0.229776 4.2:+1246479 MS.gene62674:CDS
AAGCCACTCTTCACTGTTAC+TGG 0.259229 4.2:-1246557 None:intergenic
GGATGACACAGATATCTTTA+AGG 0.266657 4.2:+1245086 MS.gene62674:CDS
AGGTGGTGGGGAATTGGTTT+TGG 0.266949 4.2:+1245726 MS.gene62674:CDS
TGCTTCCTCTGAGCCTTTAT+AGG 0.269385 4.2:-1244253 None:intergenic
AATCCCAATATAACCCCTTT+TGG 0.273386 4.2:-1244908 None:intergenic
GATATTTGCTGATAGGATTA+TGG 0.280396 4.2:+1244615 MS.gene62674:CDS
GAAACTTTCTCTTCAGTCTT+TGG 0.280880 4.2:-1245012 None:intergenic
GAATCCAAAAGGGGTTATAT+TGG 0.281794 4.2:+1244904 MS.gene62674:CDS
GTACTCATGATGTCGGTTAC+TGG 0.300060 4.2:+1245649 MS.gene62674:CDS
AAGAGGATTAATGAAAGATT+TGG 0.300946 4.2:+1245258 MS.gene62674:CDS
CCAAGGATTATGACTGTTGC+TGG 0.303200 4.2:+1244127 MS.gene62674:CDS
ATCCAAACATAATCATCTTC+AGG 0.313897 4.2:-1244649 None:intergenic
GAAACCTCATCTTGCTCATT+TGG 0.314431 4.2:-1244406 None:intergenic
TATATTGGGATTGAGTATTA+TGG 0.314572 4.2:+1244919 MS.gene62674:CDS
AGGAGAAGGTGGTGGGGAAT+TGG 0.321169 4.2:+1245720 MS.gene62674:CDS
GTGCCTAAGATAAAACTATT+TGG 0.324753 4.2:-1247565 None:intergenic
TAAATCTCTGATGAAGGAAA+TGG 0.327298 4.2:-1244754 None:intergenic
CTCAAAAGCTCTTCCCTAAT+AGG 0.330353 4.2:-1244787 None:intergenic
CCTGAATTTGTTCTGTGTAT+TGG 0.331756 4.2:+1247365 MS.gene62674:CDS
CCGTAGCTTCTTGCAAGATT+TGG 0.337619 4.2:+1245674 MS.gene62674:CDS
TTGGTTGTGTCTGAGTTTAT+TGG 0.338247 4.2:+1245489 MS.gene62674:CDS
CTGGTCCTTCCATGGCTCCT+AGG 0.341786 4.2:+1247432 MS.gene62674:CDS
TTTGAGGTCATCACTAGTTC+TGG 0.342484 4.2:+1247413 MS.gene62674:CDS
TTACAAGACATTGCCTATTA+GGG 0.349312 4.2:+1244774 MS.gene62674:CDS
AATCCAAAAGGGGTTATATT+GGG 0.361586 4.2:+1244905 MS.gene62674:CDS
ATGGTAATAACATGAATAAA+TGG 0.364353 4.2:+1244278 MS.gene62674:CDS
GGCTAATCAACTTCCTATAA+AGG 0.366306 4.2:+1244240 MS.gene62674:CDS
TTGCCTCTGTCACCAGGCCT+TGG 0.371449 4.2:+1244544 MS.gene62674:CDS
TCAATAAGATATTTGCTGAT+AGG 0.374227 4.2:+1244608 MS.gene62674:CDS
GCTTGGCATGATCCAAAATC+AGG 0.379292 4.2:-1246491 None:intergenic
ACCATTCAGTAAGCGCCTTT+CGG 0.381194 4.2:-1245977 None:intergenic
CGTGGATCCATCTGTTGTTT+CGG 0.383516 4.2:-1246420 None:intergenic
CCAATACACAGAACAAATTC+AGG 0.383583 4.2:-1247365 None:intergenic
ACCCTGAAGATGATTATGTT+TGG 0.386374 4.2:+1244647 MS.gene62674:CDS
GTTGTTTGGTGACTGCAGTT+AGG 0.386829 4.2:+1245364 MS.gene62674:CDS
TGTGGCTAAGTGCCTACTTT+CGG 0.390454 4.2:+1247319 MS.gene62674:CDS
GCCTCAGGCTTCCATTGATA+AGG 0.396543 4.2:+1245872 MS.gene62674:CDS
TATTTATGTTGGATGTCTTA+AGG 0.405849 4.2:+1244372 MS.gene62674:CDS
CTTCTACTTCAACTCAAAGA+TGG 0.409979 4.2:+1244325 MS.gene62674:CDS
ATTTCCTATCATGTTGTAGT+AGG 0.415880 4.2:+1244863 MS.gene62674:CDS
GATGACACAGATATCTTTAA+GGG 0.417700 4.2:+1245087 MS.gene62674:CDS
AAGTAGTACTTGGCCTTGCT+GGG 0.418154 4.2:-1247488 None:intergenic
ATAAGGAGACAGCACTTGTT+TGG 0.418945 4.2:+1246450 MS.gene62674:CDS
TCCTTCCCTTTCCCTCGTGC+AGG 0.421069 4.2:-1245198 None:intergenic
CGTTGTAGCTGAGTGTTGTT+TGG 0.422680 4.2:+1245350 MS.gene62674:CDS
CTTACAAGCAGACGAAAACT+AGG 0.423719 4.2:+1245817 MS.gene62674:CDS
CAATCAAATCAGAATTCAAA+AGG 0.424700 4.2:-1244813 None:intergenic
GATACTGATGTTATTTATGT+TGG 0.425721 4.2:+1244361 MS.gene62674:CDS
CCTCTGTCACCAGGCCTTGG+TGG 0.427112 4.2:+1244547 MS.gene62674:CDS
CTGCACCATTCTCACTATAT+CGG 0.436393 4.2:-1247522 None:intergenic
TTGTTTGGTGACTGCAGTTA+GGG 0.442313 4.2:+1245365 MS.gene62674:CDS
GCATCCTACTACAACATGAT+AGG 0.442394 4.2:-1244867 None:intergenic
TGATGCAATGGACTCAGCTT+TGG 0.442527 4.2:+1245572 MS.gene62674:CDS
GTCTATCCTATGAATCCAAA+AGG 0.443152 4.2:+1244893 MS.gene62674:CDS
AATGAAAGATTTGGAAAGCC+TGG 0.444274 4.2:+1245267 MS.gene62674:CDS
GAGTGCCATCGTAATCTAGA+AGG 0.445396 4.2:-1245845 None:intergenic
CGACTGATTATATATTCATA+AGG 0.447308 4.2:-1245405 None:intergenic
CTTCAACTCAAAGATGGGTT+AGG 0.449220 4.2:+1244331 MS.gene62674:CDS
CTTTCTCTTCAGTCTTTGGT+AGG 0.450253 4.2:-1245008 None:intergenic
GCCTTGGTGGTAGGTTCAAT+AGG 0.451958 4.2:+1244560 MS.gene62674:CDS
CAACGTTTGAAAACATCAAC+AGG 0.453156 4.2:+1244098 MS.gene62674:CDS
ACTTTCGGCCATGCAAGAAA+AGG 0.456881 4.2:+1247334 MS.gene62674:CDS
TGATGTTGATGATGACCCTT+TGG 0.457651 4.2:+1244159 MS.gene62674:CDS
TGTCACCAGGCCTTGGTGGT+AGG 0.457812 4.2:+1244551 MS.gene62674:CDS
AACTATTTGGTCTGAAACAC+AGG 0.458415 4.2:-1247552 None:intergenic
CCAAATCTTGCAAGAAGCTA+CGG 0.467585 4.2:-1245674 None:intergenic
CTTTGAACAGATGAGGAAGA+AGG 0.471489 4.2:-1244199 None:intergenic
CAGCAAGGCCAAGTACTACT+TGG 0.472261 4.2:+1247490 MS.gene62674:CDS
AACCTCTTCCTCAAGAAAGT+TGG 0.473764 4.2:-1244697 None:intergenic
CCAGCAACAGTCATAATCCT+TGG 0.474830 4.2:-1244127 None:intergenic
CACTAGTTCTGGTCCTTCCA+TGG 0.474911 4.2:+1247424 MS.gene62674:CDS
CGATATAGTGAGAATGGTGC+AGG 0.476585 4.2:+1247523 MS.gene62674:CDS
TGACCCATGTGTATGCCAAC+AGG 0.477648 4.2:-1244967 None:intergenic
GGTTGTTCACCATCTTTAAG+TGG 0.482976 4.2:+1245510 MS.gene62674:CDS
CCTCAGGCTTCCATTGATAA+GGG 0.483342 4.2:+1245873 MS.gene62674:CDS
TACCAACTTTCTTGAGGAAG+AGG 0.485361 4.2:+1244695 MS.gene62674:CDS
TACTCATGATGTCGGTTACT+GGG 0.485413 4.2:+1245650 MS.gene62674:CDS
TGGTCCTTCCATGGCTCCTA+GGG 0.485852 4.2:+1247433 MS.gene62674:CDS
TGATCCTGTCATTCTGATTG+AGG 0.485986 4.2:+1245293 MS.gene62674:CDS
TGGATCCACGATTGAAGATA+AGG 0.486239 4.2:+1246433 MS.gene62674:CDS
AATGGACTCAGCTTTGGAAA+TGG 0.486505 4.2:+1245578 MS.gene62674:CDS
GTTCAATAGGTCACTGTGGC+AGG 0.488428 4.2:+1244573 MS.gene62674:CDS
ACTGAAGCGGGATGCAGAAT+GGG 0.489562 4.2:+1246331 MS.gene62674:CDS
GGCTCCTCAATCAGAATGAC+AGG 0.489606 4.2:-1245297 None:intergenic
GCCGAAAGGCGCTTACTGAA+TGG 0.491778 4.2:+1245976 MS.gene62674:CDS
GATTAAGAAGTTCTTTAGCT+TGG 0.493640 4.2:-1246508 None:intergenic
CTAATCCTGCACGAGGGAAA+GGG 0.497486 4.2:+1245193 MS.gene62674:CDS
TCTATCCTATGAATCCAAAA+GGG 0.498749 4.2:+1244894 MS.gene62674:CDS
GCTAATCCTGCACGAGGGAA+AGG 0.499260 4.2:+1245192 MS.gene62674:CDS
AAGTTTGAAGTTGTTAGCCA+TGG 0.502578 4.2:+1245113 MS.gene62674:CDS
AGTATCAAAATTCTTCCTGT+TGG 0.503713 4.2:+1244952 MS.gene62674:CDS
TACATGTTGCCTCTGTCACC+AGG 0.504717 4.2:+1244538 MS.gene62674:CDS
GACTGAAGCGGGATGCAGAA+TGG 0.504876 4.2:+1246330 MS.gene62674:intron
TTTCTTGCATGGCCGAAAGT+AGG 0.505526 4.2:-1247331 None:intergenic
CGATGGCACTCTAATGCCTC+AGG 0.508745 4.2:+1245857 MS.gene62674:CDS
TACTTCAGCCCTAGGAGCCA+TGG 0.509397 4.2:-1247441 None:intergenic
ACTTATTGGACCCTTATCAA+TGG 0.511323 4.2:-1245883 None:intergenic
CTTTCGGCCATGCAAGAAAA+GGG 0.511483 4.2:+1247335 MS.gene62674:CDS
TCCAAACATAATCATCTTCA+GGG 0.512166 4.2:-1244648 None:intergenic
TTCTACTTCAACTCAAAGAT+GGG 0.513201 4.2:+1244326 MS.gene62674:CDS
GCACCCTGAGTGGCATGAAA+AGG 0.513329 4.2:+1245152 MS.gene62674:CDS
AGATCACCTGGGAGAAAAGT+AGG 0.513575 4.2:-1244460 None:intergenic
GGATTTGTAGTGATCATGTA+AGG 0.518174 4.2:+1245700 MS.gene62674:CDS
CAAATCTTGCAAGAAGCTAC+GGG 0.520883 4.2:-1245673 None:intergenic
GTGGCATGAAAAGGTTGTAT+TGG 0.523521 4.2:+1245161 MS.gene62674:CDS
GTGACAGAGGCAACATGTAA+TGG 0.523789 4.2:-1244534 None:intergenic
AAATCTCTGATGAAGGAAAT+GGG 0.525502 4.2:-1244753 None:intergenic
GTCTTGTAAATCTCTGATGA+AGG 0.525942 4.2:-1244760 None:intergenic
CACCTGTAGCAGACTTCAGT+TGG 0.526677 4.2:+1246363 MS.gene62674:CDS
TCATGTTGTAGTAGGATGCT+TGG 0.527256 4.2:+1244871 MS.gene62674:CDS
TTCCAACTGAAGTCTGCTAC+AGG 0.529777 4.2:-1246365 None:intergenic
GATACATGATTATCATCTCA+TGG 0.533172 4.2:+1244669 MS.gene62674:CDS
ATCATGTAAGGAGAAGGTGG+TGG 0.533579 4.2:+1245712 MS.gene62674:CDS
ATCTGATGAAGACATGTTTG+AGG 0.533716 4.2:+1247397 MS.gene62674:CDS
AAAGATAATGTTACTTGGTG+TGG 0.533734 4.2:+1245065 MS.gene62674:CDS
CATGCAAATACTTCAGCCCT+AGG 0.534052 4.2:-1247449 None:intergenic
AAGAGAGAGGATCATAATAG+TGG 0.534843 4.2:+1244219 MS.gene62674:CDS
CTGATAGCTCCACTTAAAGA+TGG 0.537544 4.2:-1245519 None:intergenic
TCATGTAAGGAGAAGGTGGT+GGG 0.540099 4.2:+1245713 MS.gene62674:CDS
CTGATGAAGGAAATGGGCTG+TGG 0.540371 4.2:-1244747 None:intergenic
GAAGTATTTGCATGCACTGT+TGG 0.540517 4.2:+1247458 MS.gene62674:CDS
CTGAATAAGAAGCTGCTCCA+TGG 0.541635 4.2:-1245130 None:intergenic
GTATGGTTTCAGACTGAAGC+GGG 0.545907 4.2:+1246319 MS.gene62674:intron
GCTTCTTGCAAGATTTGGAA+AGG 0.545943 4.2:+1245679 MS.gene62674:CDS
ACACATGCAGGGTGTGAACA+AGG 0.548293 4.2:+1247292 MS.gene62674:intron
AATCTACTAGAGCTAGTCTC+TGG 0.553158 4.2:+1244067 MS.gene62674:CDS
GATGATGACCCTTTGGAGAG+TGG 0.553229 4.2:+1244166 MS.gene62674:CDS
AGGTTTAATAGAGTGAAACT+TGG 0.553468 4.2:+1244715 MS.gene62674:CDS
TTCTGATTGAGGAGCCATTG+AGG 0.558923 4.2:+1245304 MS.gene62674:CDS
TTGAACCTACCACCAAGGCC+TGG 0.561132 4.2:-1244556 None:intergenic
CATGCTCAGCAGCAATCCCC+AGG 0.562276 4.2:-1246016 None:intergenic
TTTCTCCTTGCGAAAACCTG+GGG 0.562838 4.2:+1246000 MS.gene62674:CDS
GAGCTATCAGAGTGAATCCA+TGG 0.565285 4.2:+1245532 MS.gene62674:CDS
CTGGGGATTGCTGCTGAGCA+TGG 0.565464 4.2:+1246017 MS.gene62674:CDS
AGTGTCATCCAAGTAGTACT+TGG 0.566276 4.2:-1247498 None:intergenic
GCTACTGCATCAATATTCCA+TGG 0.566583 4.2:-1245549 None:intergenic
CACTGCCGATATAGTGAGAA+TGG 0.566955 4.2:+1247517 MS.gene62674:CDS
AATCAAATCAGAATTCAAAA+GGG 0.571439 4.2:-1244812 None:intergenic
CAGAACAACCACTCTCCAAA+GGG 0.573092 4.2:-1244174 None:intergenic
TGGTGTTACCAACTTTCTTG+AGG 0.574083 4.2:+1244689 MS.gene62674:CDS
CCTCATCTGTTCAAAGAGAG+AGG 0.578913 4.2:+1244206 MS.gene62674:CDS
CCTCTCTCTTTGAACAGATG+AGG 0.579822 4.2:-1244206 None:intergenic
AAAGGAAAGATAATGTTACT+TGG 0.580891 4.2:+1245060 MS.gene62674:CDS
CTGATTCAGAGAAACAGCTT+AGG 0.583665 4.2:+1245601 MS.gene62674:CDS
TTTGAACAGATGAGGAAGAA+GGG 0.587497 4.2:-1244198 None:intergenic
TAATGTGGAGGTTAAACCAC+AGG 0.590016 4.2:+1246583 MS.gene62674:CDS
TCTTCCTGTTGGCATACACA+TGG 0.591080 4.2:+1244963 MS.gene62674:CDS
GTCTTGCATAATCAAAAGTG+TGG 0.591403 4.2:-1244840 None:intergenic
TCAGAACAACCACTCTCCAA+AGG 0.591725 4.2:-1244175 None:intergenic
TTGGTGACTGCAGTTAGGGA+TGG 0.592851 4.2:+1245369 MS.gene62674:CDS
AGACAAAAGCTACGGCGAAG+AGG 0.594739 4.2:+1245241 MS.gene62674:CDS
TCGGTGTAAAGCTTCATCAC+AGG 0.600827 4.2:-1246401 None:intergenic
CTTCCTGTTGGCATACACAT+GGG 0.603940 4.2:+1244964 MS.gene62674:CDS
TATGTTAGTACTCATGATGT+CGG 0.604186 4.2:+1245642 MS.gene62674:CDS
CATGTAAGGAGAAGGTGGTG+GGG 0.605812 4.2:+1245714 MS.gene62674:CDS
GTAGTGATCATGTAAGGAGA+AGG 0.606006 4.2:+1245706 MS.gene62674:CDS
ATTTGCTGATAGGATTATGG+AGG 0.608159 4.2:+1244618 MS.gene62674:CDS
TTGGGATTGAGTATTATGGT+AGG 0.613646 4.2:+1244923 MS.gene62674:CDS
CCACCAAGGCCTGGTGACAG+AGG 0.613836 4.2:-1244547 None:intergenic
TTCCTCTAAGAAAAGCATGT+TGG 0.614570 4.2:+1245470 MS.gene62674:CDS
CACATGCAGGGTGTGAACAA+GGG 0.617510 4.2:+1247293 MS.gene62674:intron
CAGATAGCTAATCCTGCACG+AGG 0.619839 4.2:+1245186 MS.gene62674:CDS
GAATATATAATCAGTCGTCA+AGG 0.621419 4.2:+1245411 MS.gene62674:CDS
TTCTTATTCAGCACCCTGAG+TGG 0.624533 4.2:+1245142 MS.gene62674:CDS
ACATCAACAGGAGAATTCCA+AGG 0.626195 4.2:+1244110 MS.gene62674:CDS
TGATGCAGTAGCTGATGCAA+TGG 0.626363 4.2:+1245560 MS.gene62674:CDS
GCAAGCCGAGACAAAAGCTA+CGG 0.632384 4.2:+1245233 MS.gene62674:CDS
CTTTACACCGAAACAACAGA+TGG 0.633557 4.2:+1246413 MS.gene62674:CDS
AGGGAAAGGGAAGGATGTGA+AGG 0.634035 4.2:+1245206 MS.gene62674:CDS
CTATCCTATGAATCCAAAAG+GGG 0.639551 4.2:+1244895 MS.gene62674:CDS
GGGTGTGAACAAGGGACTTG+TGG 0.642436 4.2:+1247301 MS.gene62674:intron
TCATCCAAATGAGCAAGATG+AGG 0.645265 4.2:+1244402 MS.gene62674:CDS
AAGGCTCAGAGGAAGCAAGA+TGG 0.645296 4.2:+1244259 MS.gene62674:CDS
TTACAAGCAGACGAAAACTA+GGG 0.645980 4.2:+1245818 MS.gene62674:CDS
GTGATCATGTAAGGAGAAGG+TGG 0.648395 4.2:+1245709 MS.gene62674:CDS
TCCTGCACGAGGGAAAGGGA+AGG 0.651339 4.2:+1245197 MS.gene62674:CDS
TGTATGGTTTCAGACTGAAG+CGG 0.652924 4.2:+1246318 MS.gene62674:intron
CCCTTATCAATGGAAGCCTG+AGG 0.657525 4.2:-1245873 None:intergenic
AGAATGACAGGATCATAACC+AGG 0.659389 4.2:-1245285 None:intergenic
AACTTCCTATAAAGGCTCAG+AGG 0.663406 4.2:+1244248 MS.gene62674:CDS
AGTAAGTGCTAAAAGCCGAA+AGG 0.665875 4.2:+1245962 MS.gene62674:CDS
TGTTGGTCGAAAACCCAGCA+AGG 0.667193 4.2:+1247475 MS.gene62674:CDS
GGCAACATGTAATGGAACAG+TGG 0.669575 4.2:-1244526 None:intergenic
AGCACTTGTTTGGTGCTACG+AGG 0.670344 4.2:+1246460 MS.gene62674:CDS
TCAGCCCTAGGAGCCATGGA+AGG 0.671674 4.2:-1247437 None:intergenic
GAAGAGTGGCTTGAATAATG+TGG 0.677280 4.2:+1246568 MS.gene62674:CDS
GAACCAGTAACAGTGAAGAG+TGG 0.680785 4.2:+1246554 MS.gene62674:CDS
TGTCTCCTTATCTTCAATCG+TGG 0.682762 4.2:-1246438 None:intergenic
ATGACACAGATATCTTTAAG+GGG 0.687759 4.2:+1245088 MS.gene62674:CDS
ACCTATTGAACCTACCACCA+AGG 0.689096 4.2:-1244561 None:intergenic
GATATAGTGAGAATGGTGCA+GGG 0.698860 4.2:+1247524 MS.gene62674:CDS
GCGATCCTTCTAGATTACGA+TGG 0.708503 4.2:+1245840 MS.gene62674:CDS
GAGTGGCTTGAATAATGTGG+AGG 0.723673 4.2:+1246571 MS.gene62674:CDS
AGATAGCTAATCCTGCACGA+GGG 0.749112 4.2:+1245187 MS.gene62674:CDS
GTGTAGAGATGACAACAACA+TGG 0.765307 4.2:+1245932 MS.gene62674:CDS
GTAGGTTCAATAGGTCACTG+TGG 0.787596 4.2:+1244569 MS.gene62674:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAATGAATATAAACTATAA+AGG + chr4.2:1247111-1247130 MS.gene62674:intron 15.0%
!! TATTAAAAATCTTTGATAGA+TGG + chr4.2:1246887-1246906 MS.gene62674:intron 15.0%
!!! ACTTTTTAAGGTAAATTATT+TGG + chr4.2:1246042-1246061 MS.gene62674:intron 15.0%
!!! ATATTCATTCTTTTACTAAA+AGG - chr4.2:1247102-1247121 None:intergenic 15.0%
!!! ATTTTAATGGAATTACTTAT+TGG - chr4.2:1245900-1245919 None:intergenic 15.0%
!!! CTTTTTAAGGTAAATTATTT+GGG + chr4.2:1246043-1246062 MS.gene62674:intron 15.0%
!!! TTTTTAAGGTAAATTATTTG+GGG + chr4.2:1246044-1246063 MS.gene62674:intron 15.0%
!! AATCAAATCAGAATTCAAAA+GGG - chr4.2:1244815-1244834 None:intergenic 20.0%
!! ATAAACTATAAAGGTAACTT+AGG + chr4.2:1247120-1247139 MS.gene62674:intron 20.0%
!! ATGGTAATAACATGAATAAA+TGG + chr4.2:1244278-1244297 MS.gene62674:CDS 20.0%
!! CATGTTTAATATTATGTCAA+GGG + chr4.2:1246851-1246870 MS.gene62674:intron 20.0%
!! TAAACTATAAAGGTAACTTA+GGG + chr4.2:1247121-1247140 MS.gene62674:intron 20.0%
!! TATATAATCACTTTCTGTAA+TGG - chr4.2:1247152-1247171 None:intergenic 20.0%
!! TCATGTTTAATATTATGTCA+AGG + chr4.2:1246850-1246869 MS.gene62674:intron 20.0%
!!! ATACAACATTTTTCTGATAA+AGG + chr4.2:1245042-1245061 MS.gene62674:CDS 20.0%
!!! ATGATAAATTTTCTGTCTAT+TGG + chr4.2:1246645-1246664 MS.gene62674:intron 20.0%
!!! CAAACTATTAAGCATTTTAA+TGG - chr4.2:1245913-1245932 None:intergenic 20.0%
! AAAAGGAAAAGATTGAATCA+AGG - chr4.2:1247085-1247104 None:intergenic 25.0%
! AAACTTCAAAAAGCTTTCAA+TGG + chr4.2:1245779-1245798 MS.gene62674:CDS 25.0%
! AAAGGAAAGATAATGTTACT+TGG + chr4.2:1245060-1245079 MS.gene62674:CDS 25.0%
! AAGAGGATTAATGAAAGATT+TGG + chr4.2:1245258-1245277 MS.gene62674:CDS 25.0%
! AATAGCTGTTAAATTGAGAT+CGG - chr4.2:1246214-1246233 None:intergenic 25.0%
! ATACATATATATACACATGC+AGG + chr4.2:1247280-1247299 MS.gene62674:intron 25.0%
! CAATCAAATCAGAATTCAAA+AGG - chr4.2:1244816-1244835 None:intergenic 25.0%
! CGACTGATTATATATTCATA+AGG - chr4.2:1245408-1245427 None:intergenic 25.0%
! GTTAGATTATAACTTACAGT+TGG + chr4.2:1246293-1246312 MS.gene62674:intron 25.0%
! TACATATATATACACATGCA+GGG + chr4.2:1247281-1247300 MS.gene62674:intron 25.0%
! TATATTGGGATTGAGTATTA+TGG + chr4.2:1244919-1244938 MS.gene62674:CDS 25.0%
! TATTTATGTTGGATGTCTTA+AGG + chr4.2:1244372-1244391 MS.gene62674:CDS 25.0%
! TGTATATGAATATGCATTAG+AGG - chr4.2:1247265-1247284 None:intergenic 25.0%
!! GAAAAATTGGACAAAGTTTT+GGG + chr4.2:1245438-1245457 MS.gene62674:CDS 25.0%
!! GATACTGATGTTATTTATGT+TGG + chr4.2:1244361-1244380 MS.gene62674:CDS 25.0%
!! TCAATAAGATATTTGCTGAT+AGG + chr4.2:1244608-1244627 MS.gene62674:CDS 25.0%
!! TGAAAAATTGGACAAAGTTT+TGG + chr4.2:1245437-1245456 MS.gene62674:CDS 25.0%
!!! ACTTTTTCATAAAACCTCAA+TGG - chr4.2:1245321-1245340 None:intergenic 25.0%
!!! ATTGAAAGCTTTTTGAAGTT+TGG - chr4.2:1245780-1245799 None:intergenic 25.0%
!!! CTTTTGAATTCTGATTTGAT+TGG + chr4.2:1244814-1244833 MS.gene62674:CDS 25.0%
!!! CTTTTTACAAGGTATTATCA+TGG + chr4.2:1244481-1244500 MS.gene62674:CDS 25.0%
!!! GAATAAATGGTTTTTCAGTT+GGG + chr4.2:1244291-1244310 MS.gene62674:CDS 25.0%
!!! TGAATAAATGGTTTTTCAGT+TGG + chr4.2:1244290-1244309 MS.gene62674:CDS 25.0%
AAAACAAAGTTCCTTTACTG+TGG + chr4.2:1246760-1246779 MS.gene62674:intron 30.0%
AAATCTCTGATGAAGGAAAT+GGG - chr4.2:1244756-1244775 None:intergenic 30.0%
AATCCAAAAGGGGTTATATT+GGG + chr4.2:1244905-1244924 MS.gene62674:CDS 30.0%
AGGTTTAATAGAGTGAAACT+TGG + chr4.2:1244715-1244734 MS.gene62674:CDS 30.0%
AGTATCAAAATTCTTCCTGT+TGG + chr4.2:1244952-1244971 MS.gene62674:CDS 30.0%
ATCATTTCTCAAACACAACA+AGG - chr4.2:1247204-1247223 None:intergenic 30.0%
ATCCAAACATAATCATCTTC+AGG - chr4.2:1244652-1244671 None:intergenic 30.0%
ATGACACAGATATCTTTAAG+GGG + chr4.2:1245088-1245107 MS.gene62674:CDS 30.0%
ATTTCCTATCATGTTGTAGT+AGG + chr4.2:1244863-1244882 MS.gene62674:CDS 30.0%
GAATATATAATCAGTCGTCA+AGG + chr4.2:1245411-1245430 MS.gene62674:CDS 30.0%
GATACATGATTATCATCTCA+TGG + chr4.2:1244669-1244688 MS.gene62674:CDS 30.0%
GATGACACAGATATCTTTAA+GGG + chr4.2:1245087-1245106 MS.gene62674:CDS 30.0%
GATTAAGAAGTTCTTTAGCT+TGG - chr4.2:1246511-1246530 None:intergenic 30.0%
TAAATCTCTGATGAAGGAAA+TGG - chr4.2:1244757-1244776 None:intergenic 30.0%
TATGTTAGTACTCATGATGT+CGG + chr4.2:1245642-1245661 MS.gene62674:CDS 30.0%
TCCAAACATAATCATCTTCA+GGG - chr4.2:1244651-1244670 None:intergenic 30.0%
TCGTCAAGGAAATGAAAAAT+TGG + chr4.2:1245425-1245444 MS.gene62674:CDS 30.0%
TCTATCCTATGAATCCAAAA+GGG + chr4.2:1244894-1244913 MS.gene62674:CDS 30.0%
TGTTTGAGAAATGATAAGCT+TGG + chr4.2:1247209-1247228 MS.gene62674:intron 30.0%
TTACAAGACATTGCCTATTA+GGG + chr4.2:1244774-1244793 MS.gene62674:CDS 30.0%
TTCTACTTCAACTCAAAGAT+GGG + chr4.2:1244326-1244345 MS.gene62674:CDS 30.0%
TTTACAAGACATTGCCTATT+AGG + chr4.2:1244773-1244792 MS.gene62674:CDS 30.0%
! AAAGATAATGTTACTTGGTG+TGG + chr4.2:1245065-1245084 MS.gene62674:CDS 30.0%
! ATTTTCTGTCTATTGGATTC+TGG + chr4.2:1246652-1246671 MS.gene62674:intron 30.0%
! GATATTTGCTGATAGGATTA+TGG + chr4.2:1244615-1244634 MS.gene62674:CDS 30.0%
! TTTTAAACTCTAAACTACCG+AGG - chr4.2:1246171-1246190 None:intergenic 30.0%
!! AGAAGTATCAAACCATGAAA+AGG - chr4.2:1246273-1246292 None:intergenic 30.0%
!! CTTATGACATTTTCAGGATT+TGG + chr4.2:1246067-1246086 MS.gene62674:intron 30.0%
AAGAGAGAGGATCATAATAG+TGG + chr4.2:1244219-1244238 MS.gene62674:CDS 35.0%
AATGAAAGATTTGGAAAGCC+TGG + chr4.2:1245267-1245286 MS.gene62674:CDS 35.0%
ACCCTGAAGATGATTATGTT+TGG + chr4.2:1244647-1244666 MS.gene62674:CDS 35.0%
ACCTTGTAAAAAGATCACCT+GGG - chr4.2:1244474-1244493 None:intergenic 35.0%
ACTTATTGGACCCTTATCAA+TGG - chr4.2:1245886-1245905 None:intergenic 35.0%
ATCTGATGAAGACATGTTTG+AGG + chr4.2:1247397-1247416 MS.gene62674:CDS 35.0%
ATGTAACTTAGGGTTGCATT+TGG + chr4.2:1246914-1246933 MS.gene62674:intron 35.0%
CAACGTTTGAAAACATCAAC+AGG + chr4.2:1244098-1244117 MS.gene62674:CDS 35.0%
CCAATACACAGAACAAATTC+AGG - chr4.2:1247368-1247387 None:intergenic 35.0%
CTATCCTATGAATCCAAAAG+GGG + chr4.2:1244895-1244914 MS.gene62674:CDS 35.0%
CTTCTACTTCAACTCAAAGA+TGG + chr4.2:1244325-1244344 MS.gene62674:CDS 35.0%
GAAACTTTCTCTTCAGTCTT+TGG - chr4.2:1245015-1245034 None:intergenic 35.0%
GAATCCAAAAGGGGTTATAT+TGG + chr4.2:1244904-1244923 MS.gene62674:CDS 35.0%
GGATGACACAGATATCTTTA+AGG + chr4.2:1245086-1245105 MS.gene62674:CDS 35.0%
GGATTTGTAGTGATCATGTA+AGG + chr4.2:1245700-1245719 MS.gene62674:CDS 35.0%
GGCTAATCAACTTCCTATAA+AGG + chr4.2:1244240-1244259 MS.gene62674:CDS 35.0%
GGTTTGCAAATTCACATAGT+CGG - chr4.2:1246243-1246262 None:intergenic 35.0%
GTCTATCCTATGAATCCAAA+AGG + chr4.2:1244893-1244912 MS.gene62674:CDS 35.0%
GTCTTGCATAATCAAAAGTG+TGG - chr4.2:1244843-1244862 None:intergenic 35.0%
GTCTTGTAAATCTCTGATGA+AGG - chr4.2:1244763-1244782 None:intergenic 35.0%
GTGACTGTTAGATGAAAGAT+CGG + chr4.2:1246129-1246148 MS.gene62674:intron 35.0%
TACCTTGTAAAAAGATCACC+TGG - chr4.2:1244475-1244494 None:intergenic 35.0%
TTACAAGCAGACGAAAACTA+GGG + chr4.2:1245818-1245837 MS.gene62674:CDS 35.0%
TTGGGATTGAGTATTATGGT+AGG + chr4.2:1244923-1244942 MS.gene62674:CDS 35.0%
TTGGTTGTGTCTGAGTTTAT+TGG + chr4.2:1245489-1245508 MS.gene62674:CDS 35.0%
TTTGAACAGATGAGGAAGAA+GGG - chr4.2:1244201-1244220 None:intergenic 35.0%
! AAACCAGATACACCTTTTCA+TGG + chr4.2:1246258-1246277 MS.gene62674:intron 35.0%
! AAACCATGAAAAGGTGTATC+TGG - chr4.2:1246264-1246283 None:intergenic 35.0%
! AACCAACATGCTTTTCTTAG+AGG - chr4.2:1245475-1245494 None:intergenic 35.0%
! AACTATTTGGTCTGAAACAC+AGG - chr4.2:1247555-1247574 None:intergenic 35.0%
! AAGTTTGAAGTTGTTAGCCA+TGG + chr4.2:1245113-1245132 MS.gene62674:CDS 35.0%
! AATCCCAATATAACCCCTTT+TGG - chr4.2:1244911-1244930 None:intergenic 35.0%
! AGATGGAAGCATGTAACTTA+GGG + chr4.2:1246904-1246923 MS.gene62674:intron 35.0%
! ATAACCCCTTTTGGATTCAT+AGG - chr4.2:1244902-1244921 None:intergenic 35.0%
! ATTTGCTGATAGGATTATGG+AGG + chr4.2:1244618-1244637 MS.gene62674:CDS 35.0%
! CCTGAATTTGTTCTGTGTAT+TGG + chr4.2:1247365-1247384 MS.gene62674:CDS 35.0%
! CTGTTGATGTTTTCAAACGT+TGG - chr4.2:1244100-1244119 None:intergenic 35.0%
! TAGATGGAAGCATGTAACTT+AGG + chr4.2:1246903-1246922 MS.gene62674:intron 35.0%
! TTCCTCTAAGAAAAGCATGT+TGG + chr4.2:1245470-1245489 MS.gene62674:CDS 35.0%
!! TAACTTACAGTTGGCTTGTA+TGG + chr4.2:1246302-1246321 MS.gene62674:intron 35.0%
!!! CTGAGCATGGTTACTTTTTA+AGG + chr4.2:1246030-1246049 MS.gene62674:CDS 35.0%
!!! TTGGACAAAGTTTTGGGTAT+AGG + chr4.2:1245444-1245463 MS.gene62674:CDS 35.0%
AACCTCTTCCTCAAGAAAGT+TGG - chr4.2:1244700-1244719 None:intergenic 40.0%
AACTTCCTATAAAGGCTCAG+AGG + chr4.2:1244248-1244267 MS.gene62674:CDS 40.0%
ACAAATGTTATACGCACCTG+TGG - chr4.2:1246602-1246621 None:intergenic 40.0%
ACATCAACAGGAGAATTCCA+AGG + chr4.2:1244110-1244129 MS.gene62674:CDS 40.0%
ACTTACGATGAAGATACAGC+AGG + chr4.2:1246787-1246806 MS.gene62674:intron 40.0%
AGAATGACAGGATCATAACC+AGG - chr4.2:1245288-1245307 None:intergenic 40.0%
AGCCAGTGTAAGATTGAACT+AGG - chr4.2:1247036-1247055 None:intergenic 40.0%
AGTAAGTGCTAAAAGCCGAA+AGG + chr4.2:1245962-1245981 MS.gene62674:CDS 40.0%
AGTGTCATCCAAGTAGTACT+TGG - chr4.2:1247501-1247520 None:intergenic 40.0%
ATAAGGAGACAGCACTTGTT+TGG + chr4.2:1246450-1246469 MS.gene62674:CDS 40.0%
ATCTCATGCAATCATCGCAT+CGG + chr4.2:1246107-1246126 MS.gene62674:intron 40.0%
CAAATCTTGCAAGAAGCTAC+GGG - chr4.2:1245676-1245695 None:intergenic 40.0%
CCAAATCTTGCAAGAAGCTA+CGG - chr4.2:1245677-1245696 None:intergenic 40.0%
CCAGTGTAAGATTGAACTAG+GGG - chr4.2:1247034-1247053 None:intergenic 40.0%
CGTAAGTCTAACCACAGTAA+AGG - chr4.2:1246774-1246793 None:intergenic 40.0%
CTCAAAAGCTCTTCCCTAAT+AGG - chr4.2:1244790-1244809 None:intergenic 40.0%
CTGATAGCTCCACTTAAAGA+TGG - chr4.2:1245522-1245541 None:intergenic 40.0%
CTGATTCAGAGAAACAGCTT+AGG + chr4.2:1245601-1245620 MS.gene62674:CDS 40.0%
CTGCACCATTCTCACTATAT+CGG - chr4.2:1247525-1247544 None:intergenic 40.0%
CTGTAAGACTGTAAGATGGT+TGG - chr4.2:1247242-1247261 None:intergenic 40.0%
CTGTTAGATGAAAGATCGGA+CGG + chr4.2:1246133-1246152 MS.gene62674:intron 40.0%
CTTACAAGCAGACGAAAACT+AGG + chr4.2:1245817-1245836 MS.gene62674:CDS 40.0%
CTTACGATGAAGATACAGCA+GGG + chr4.2:1246788-1246807 MS.gene62674:intron 40.0%
CTTTACACCGAAACAACAGA+TGG + chr4.2:1246413-1246432 MS.gene62674:CDS 40.0%
CTTTCTCTTCAGTCTTTGGT+AGG - chr4.2:1245011-1245030 None:intergenic 40.0%
CTTTGAACAGATGAGGAAGA+AGG - chr4.2:1244202-1244221 None:intergenic 40.0%
GAAACCTCATCTTGCTCATT+TGG - chr4.2:1244409-1244428 None:intergenic 40.0%
GAAGAGTGGCTTGAATAATG+TGG + chr4.2:1246568-1246587 MS.gene62674:CDS 40.0%
GACGGTCTAAATTTCAACCT+CGG + chr4.2:1246151-1246170 MS.gene62674:intron 40.0%
GATATAGTGAGAATGGTGCA+GGG + chr4.2:1247524-1247543 MS.gene62674:CDS 40.0%
GCATCCTACTACAACATGAT+AGG - chr4.2:1244870-1244889 None:intergenic 40.0%
GCCAGTGTAAGATTGAACTA+GGG - chr4.2:1247035-1247054 None:intergenic 40.0%
GCTACTGCATCAATATTCCA+TGG - chr4.2:1245552-1245571 None:intergenic 40.0%
GCTGTTAAATTGAGATCGGA+CGG - chr4.2:1246210-1246229 None:intergenic 40.0%
GCTTCTTGCAAGATTTGGAA+AGG + chr4.2:1245679-1245698 MS.gene62674:CDS 40.0%
GGTTGTTCACCATCTTTAAG+TGG + chr4.2:1245510-1245529 MS.gene62674:CDS 40.0%
GTAGTGATCATGTAAGGAGA+AGG + chr4.2:1245706-1245725 MS.gene62674:CDS 40.0%
GTGGCATGAAAAGGTTGTAT+TGG + chr4.2:1245161-1245180 MS.gene62674:CDS 40.0%
GTGTAGAGATGACAACAACA+TGG + chr4.2:1245932-1245951 MS.gene62674:CDS 40.0%
TAATGTGGAGGTTAAACCAC+AGG + chr4.2:1246583-1246602 MS.gene62674:CDS 40.0%
TACCAACTTTCTTGAGGAAG+AGG + chr4.2:1244695-1244714 MS.gene62674:CDS 40.0%
TCATCCAAATGAGCAAGATG+AGG + chr4.2:1244402-1244421 MS.gene62674:CDS 40.0%
TCATGTTGTAGTAGGATGCT+TGG + chr4.2:1244871-1244890 MS.gene62674:CDS 40.0%
TGATCCTGTCATTCTGATTG+AGG + chr4.2:1245293-1245312 MS.gene62674:CDS 40.0%
TGGATCCACGATTGAAGATA+AGG + chr4.2:1246433-1246452 MS.gene62674:CDS 40.0%
TGTATGGTTTCAGACTGAAG+CGG + chr4.2:1246318-1246337 MS.gene62674:intron 40.0%
TGTCTCCTTATCTTCAATCG+TGG - chr4.2:1246441-1246460 None:intergenic 40.0%
! AATCTACTAGAGCTAGTCTC+TGG + chr4.2:1244067-1244086 MS.gene62674:CDS 40.0%
! CTTCAACTCAAAGATGGGTT+AGG + chr4.2:1244331-1244350 MS.gene62674:CDS 40.0%
! GAAGTATTTGCATGCACTGT+TGG + chr4.2:1247458-1247477 MS.gene62674:CDS 40.0%
! GCTTTTGCAAGCAACAGTTA+TGG + chr4.2:1244503-1244522 MS.gene62674:CDS 40.0%
! TACTCATGATGTCGGTTACT+GGG + chr4.2:1245650-1245669 MS.gene62674:CDS 40.0%
! TCCCAGGTGATCTTTTTACA+AGG + chr4.2:1244470-1244489 MS.gene62674:CDS 40.0%
! TGACATTCCCTTTTCTTGCA+TGG - chr4.2:1247345-1247364 None:intergenic 40.0%
! TTGGGGCTTATGACATTTTC+AGG + chr4.2:1246061-1246080 MS.gene62674:intron 40.0%
! TTGTTTGGTGACTGCAGTTA+GGG + chr4.2:1245365-1245384 MS.gene62674:CDS 40.0%
! TTTGAGGTCATCACTAGTTC+TGG + chr4.2:1247413-1247432 MS.gene62674:CDS 40.0%
! TTTTCTCCTTGCGAAAACCT+GGG + chr4.2:1245999-1246018 MS.gene62674:CDS 40.0%
!! AATGGACTCAGCTTTGGAAA+TGG + chr4.2:1245578-1245597 MS.gene62674:CDS 40.0%
!! TGATGTTGATGATGACCCTT+TGG + chr4.2:1244159-1244178 MS.gene62674:CDS 40.0%
!! TGGTGTTACCAACTTTCTTG+AGG + chr4.2:1244689-1244708 MS.gene62674:CDS 40.0%
AAGCCACTCTTCACTGTTAC+TGG - chr4.2:1246560-1246579 None:intergenic 45.0%
ACCATTCAGTAAGCGCCTTT+CGG - chr4.2:1245980-1245999 None:intergenic 45.0%
ACCTATTGAACCTACCACCA+AGG - chr4.2:1244564-1244583 None:intergenic 45.0%
ACTTTCGGCCATGCAAGAAA+AGG + chr4.2:1247334-1247353 MS.gene62674:CDS 45.0%
AGATAGCTAATCCTGCACGA+GGG + chr4.2:1245187-1245206 MS.gene62674:CDS 45.0%
AGATCACCTGGGAGAAAAGT+AGG - chr4.2:1244463-1244482 None:intergenic 45.0%
AGGGCAAAAATGAGTTCGGA+TGG + chr4.2:1246807-1246826 MS.gene62674:intron 45.0%
ATCATGTAAGGAGAAGGTGG+TGG + chr4.2:1245712-1245731 MS.gene62674:CDS 45.0%
CACTGCCGATATAGTGAGAA+TGG + chr4.2:1247517-1247536 MS.gene62674:CDS 45.0%
CAGAACAACCACTCTCCAAA+GGG - chr4.2:1244177-1244196 None:intergenic 45.0%
CAGCAGGGCAAAAATGAGTT+CGG + chr4.2:1246803-1246822 MS.gene62674:intron 45.0%
CATGCAAATACTTCAGCCCT+AGG - chr4.2:1247452-1247471 None:intergenic 45.0%
CCAAGGATTATGACTGTTGC+TGG + chr4.2:1244127-1244146 MS.gene62674:CDS 45.0%
CCAGCAACAGTCATAATCCT+TGG - chr4.2:1244130-1244149 None:intergenic 45.0%
CCCCTAGTTCAATCTTACAC+TGG + chr4.2:1247031-1247050 MS.gene62674:intron 45.0%
CCGTAGCTTCTTGCAAGATT+TGG + chr4.2:1245674-1245693 MS.gene62674:CDS 45.0%
CCTCAGGCTTCCATTGATAA+GGG + chr4.2:1245873-1245892 MS.gene62674:CDS 45.0%
CCTCATCTGTTCAAAGAGAG+AGG + chr4.2:1244206-1244225 MS.gene62674:CDS 45.0%
CCTCTCTCTTTGAACAGATG+AGG - chr4.2:1244209-1244228 None:intergenic 45.0%
CGATATAGTGAGAATGGTGC+AGG + chr4.2:1247523-1247542 MS.gene62674:CDS 45.0%
CTGAATAAGAAGCTGCTCCA+TGG - chr4.2:1245133-1245152 None:intergenic 45.0%
CTTCCTGTTGGCATACACAT+GGG + chr4.2:1244964-1244983 MS.gene62674:CDS 45.0%
CTTTCGGCCATGCAAGAAAA+GGG + chr4.2:1247335-1247354 MS.gene62674:CDS 45.0%
GAACCAGTAACAGTGAAGAG+TGG + chr4.2:1246554-1246573 MS.gene62674:CDS 45.0%
GAGCTATCAGAGTGAATCCA+TGG + chr4.2:1245532-1245551 MS.gene62674:CDS 45.0%
GAGGCTGTAAGACTGTAAGA+TGG - chr4.2:1247246-1247265 None:intergenic 45.0%
GAGTGCCATCGTAATCTAGA+AGG - chr4.2:1245848-1245867 None:intergenic 45.0%
GAGTGGCTTGAATAATGTGG+AGG + chr4.2:1246571-1246590 MS.gene62674:CDS 45.0%
GCGATCCTTCTAGATTACGA+TGG + chr4.2:1245840-1245859 MS.gene62674:CDS 45.0%
GCTTGGCATGATCCAAAATC+AGG - chr4.2:1246494-1246513 None:intergenic 45.0%
GTAGGTTCAATAGGTCACTG+TGG + chr4.2:1244569-1244588 MS.gene62674:CDS 45.0%
GTATGGTTTCAGACTGAAGC+GGG + chr4.2:1246319-1246338 MS.gene62674:intron 45.0%
GTGACAGAGGCAACATGTAA+TGG - chr4.2:1244537-1244556 None:intergenic 45.0%
GTGATCATGTAAGGAGAAGG+TGG + chr4.2:1245709-1245728 MS.gene62674:CDS 45.0%
TCAGAACAACCACTCTCCAA+AGG - chr4.2:1244178-1244197 None:intergenic 45.0%
TCATGTAAGGAGAAGGTGGT+GGG + chr4.2:1245713-1245732 MS.gene62674:CDS 45.0%
TCGGTGTAAAGCTTCATCAC+AGG - chr4.2:1246404-1246423 None:intergenic 45.0%
TCTTCCTGTTGGCATACACA+TGG + chr4.2:1244963-1244982 MS.gene62674:CDS 45.0%
TGATGCAGTAGCTGATGCAA+TGG + chr4.2:1245560-1245579 MS.gene62674:CDS 45.0%
TGCTTCCTCTGAGCCTTTAT+AGG - chr4.2:1244256-1244275 None:intergenic 45.0%
TGTGGCTAAGTGCCTACTTT+CGG + chr4.2:1247319-1247338 MS.gene62674:CDS 45.0%
TTCCAACTGAAGTCTGCTAC+AGG - chr4.2:1246368-1246387 None:intergenic 45.0%
TTCTGATTGAGGAGCCATTG+AGG + chr4.2:1245304-1245323 MS.gene62674:CDS 45.0%
TTCTTATTCAGCACCCTGAG+TGG + chr4.2:1245142-1245161 MS.gene62674:CDS 45.0%
TTTCTCCTTGCGAAAACCTG+GGG + chr4.2:1246000-1246019 MS.gene62674:CDS 45.0%
TTTCTTGCATGGCCGAAAGT+AGG - chr4.2:1247334-1247353 None:intergenic 45.0%
! ACAACCTTTTCATGCCACTC+AGG - chr4.2:1245159-1245178 None:intergenic 45.0%
! CAACCTTTTCATGCCACTCA+GGG - chr4.2:1245158-1245177 None:intergenic 45.0%
! CGTGGATCCATCTGTTGTTT+CGG - chr4.2:1246423-1246442 None:intergenic 45.0%
! CGTTGTAGCTGAGTGTTGTT+TGG + chr4.2:1245350-1245369 MS.gene62674:CDS 45.0%
! GAGGATGCAGATCCTGATTT+TGG + chr4.2:1246479-1246498 MS.gene62674:CDS 45.0%
! GGCAACATGTAATGGAACAG+TGG - chr4.2:1244529-1244548 None:intergenic 45.0%
! GTACTCATGATGTCGGTTAC+TGG + chr4.2:1245649-1245668 MS.gene62674:CDS 45.0%
! GTTGTTTGGTGACTGCAGTT+AGG + chr4.2:1245364-1245383 MS.gene62674:CDS 45.0%
! GTTTTCTCCTTGCGAAAACC+TGG + chr4.2:1245998-1246017 MS.gene62674:CDS 45.0%
! TGATGCAATGGACTCAGCTT+TGG + chr4.2:1245572-1245591 MS.gene62674:CDS 45.0%
!! AAGTAGTACTTGGCCTTGCT+GGG - chr4.2:1247491-1247510 None:intergenic 45.0%
!! TGTGTTCCTACTTTTCTCCC+AGG + chr4.2:1244454-1244473 MS.gene62674:CDS 45.0%
AAGGCTCAGAGGAAGCAAGA+TGG + chr4.2:1244259-1244278 MS.gene62674:CDS 50.0%
ACACATGCAGGGTGTGAACA+AGG + chr4.2:1247292-1247311 MS.gene62674:intron 50.0%
ACTGAAGCGGGATGCAGAAT+GGG + chr4.2:1246331-1246350 MS.gene62674:CDS 50.0%
AGACAAAAGCTACGGCGAAG+AGG + chr4.2:1245241-1245260 MS.gene62674:CDS 50.0%
AGGGAAAGGGAAGGATGTGA+AGG + chr4.2:1245206-1245225 MS.gene62674:CDS 50.0%
CACATGCAGGGTGTGAACAA+GGG + chr4.2:1247293-1247312 MS.gene62674:intron 50.0%
CACCTGTAGCAGACTTCAGT+TGG + chr4.2:1246363-1246382 MS.gene62674:CDS 50.0%
CAGATAGCTAATCCTGCACG+AGG + chr4.2:1245186-1245205 MS.gene62674:CDS 50.0%
CAGCAAGGCCAAGTACTACT+TGG + chr4.2:1247490-1247509 MS.gene62674:CDS 50.0%
CATGTAAGGAGAAGGTGGTG+GGG + chr4.2:1245714-1245733 MS.gene62674:CDS 50.0%
CCCTTATCAATGGAAGCCTG+AGG - chr4.2:1245876-1245895 None:intergenic 50.0%
CTAATCCTGCACGAGGGAAA+GGG + chr4.2:1245193-1245212 MS.gene62674:CDS 50.0%
GCAAGCCGAGACAAAAGCTA+CGG + chr4.2:1245233-1245252 MS.gene62674:CDS 50.0%
GCCTCAGGCTTCCATTGATA+AGG + chr4.2:1245872-1245891 MS.gene62674:CDS 50.0%
GGCTCCTCAATCAGAATGAC+AGG - chr4.2:1245300-1245319 None:intergenic 50.0%
GTTCAATAGGTCACTGTGGC+AGG + chr4.2:1244573-1244592 MS.gene62674:CDS 50.0%
TACATGTTGCCTCTGTCACC+AGG + chr4.2:1244538-1244557 MS.gene62674:CDS 50.0%
TGACCCATGTGTATGCCAAC+AGG - chr4.2:1244970-1244989 None:intergenic 50.0%
TGTTGGTCGAAAACCCAGCA+AGG + chr4.2:1247475-1247494 MS.gene62674:CDS 50.0%
! CTTCGCCGTAGCTTTTGTCT+CGG - chr4.2:1245241-1245260 None:intergenic 50.0%
! GATGATGACCCTTTGGAGAG+TGG + chr4.2:1244166-1244185 MS.gene62674:CDS 50.0%
! GCCTTGGTGGTAGGTTCAAT+AGG + chr4.2:1244560-1244579 MS.gene62674:CDS 50.0%
! TTGGTGACTGCAGTTAGGGA+TGG + chr4.2:1245369-1245388 MS.gene62674:CDS 50.0%
!! AGCACTTGTTTGGTGCTACG+AGG + chr4.2:1246460-1246479 MS.gene62674:CDS 50.0%
!! CAAGTAGTACTTGGCCTTGC+TGG - chr4.2:1247492-1247511 None:intergenic 50.0%
!! CACTAGTTCTGGTCCTTCCA+TGG + chr4.2:1247424-1247443 MS.gene62674:CDS 50.0%
!! CTGATGAAGGAAATGGGCTG+TGG - chr4.2:1244750-1244769 None:intergenic 50.0%
!!! AGGTGGTGGGGAATTGGTTT+TGG + chr4.2:1245726-1245745 MS.gene62674:CDS 50.0%
CGATGGCACTCTAATGCCTC+AGG + chr4.2:1245857-1245876 MS.gene62674:CDS 55.0%
GACTGAAGCGGGATGCAGAA+TGG + chr4.2:1246330-1246349 MS.gene62674:intron 55.0%
GCACCCTGAGTGGCATGAAA+AGG + chr4.2:1245152-1245171 MS.gene62674:CDS 55.0%
GCTAATCCTGCACGAGGGAA+AGG + chr4.2:1245192-1245211 MS.gene62674:CDS 55.0%
GGGTGTGAACAAGGGACTTG+TGG + chr4.2:1247301-1247320 MS.gene62674:intron 55.0%
TACTTCAGCCCTAGGAGCCA+TGG - chr4.2:1247444-1247463 None:intergenic 55.0%
TGGTCCTTCCATGGCTCCTA+GGG + chr4.2:1247433-1247452 MS.gene62674:CDS 55.0%
TTGAACCTACCACCAAGGCC+TGG - chr4.2:1244559-1244578 None:intergenic 55.0%
! GCAATCCCCAGGTTTTCGCA+AGG - chr4.2:1246008-1246027 None:intergenic 55.0%
!! AGGAGAAGGTGGTGGGGAAT+TGG + chr4.2:1245720-1245739 MS.gene62674:CDS 55.0%
!! GCCGAAAGGCGCTTACTGAA+TGG + chr4.2:1245976-1245995 MS.gene62674:CDS 55.0%
CATGCTCAGCAGCAATCCCC+AGG - chr4.2:1246019-1246038 None:intergenic 60.0%
CTGGGGATTGCTGCTGAGCA+TGG + chr4.2:1246017-1246036 MS.gene62674:CDS 60.0%
CTGGTCCTTCCATGGCTCCT+AGG + chr4.2:1247432-1247451 MS.gene62674:CDS 60.0%
TCAGCCCTAGGAGCCATGGA+AGG - chr4.2:1247440-1247459 None:intergenic 60.0%
TCCTGCACGAGGGAAAGGGA+AGG + chr4.2:1245197-1245216 MS.gene62674:CDS 60.0%
TCCTTCCCTTTCCCTCGTGC+AGG - chr4.2:1245201-1245220 None:intergenic 60.0%
TTGCCTCTGTCACCAGGCCT+TGG + chr4.2:1244544-1244563 MS.gene62674:CDS 60.0%
! TGTCACCAGGCCTTGGTGGT+AGG + chr4.2:1244551-1244570 MS.gene62674:CDS 60.0%
CCACCAAGGCCTGGTGACAG+AGG - chr4.2:1244550-1244569 None:intergenic 65.0%
CCTCTGTCACCAGGCCTTGG+TGG + chr4.2:1244547-1244566 MS.gene62674:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 1244046 1247583 1244046 ID=MS.gene62674
chr4.2 mRNA 1244046 1247583 1244046 ID=MS.gene62674.t1;Parent=MS.gene62674
chr4.2 exon 1244046 1246051 1244046 ID=MS.gene62674.t1.exon1;Parent=MS.gene62674.t1
chr4.2 CDS 1244046 1246051 1244046 ID=cds.MS.gene62674.t1;Parent=MS.gene62674.t1
chr4.2 exon 1246331 1246604 1246331 ID=MS.gene62674.t1.exon2;Parent=MS.gene62674.t1
chr4.2 CDS 1246331 1246604 1246331 ID=cds.MS.gene62674.t1;Parent=MS.gene62674.t1
chr4.2 exon 1247302 1247583 1247302 ID=MS.gene62674.t1.exon3;Parent=MS.gene62674.t1
chr4.2 CDS 1247302 1247583 1247302 ID=cds.MS.gene62674.t1;Parent=MS.gene62674.t1
Gene Sequence

>MS.gene62674

ATGGTTTCAAGATCTTATTCAAATCTACTAGAGCTAGTCTCTGGTGAGTCTCCAACGTTTGAAAACATCAACAGGAGAATTCCAAGGATTATGACTGTTGCTGGTTTGATATCTGATGTTGATGATGACCCTTTGGAGAGTGGTTGTTCTGACCCTTCTTCCTCATCTGTTCAAAGAGAGAGGATCATAATAGTGGCTAATCAACTTCCTATAAAGGCTCAGAGGAAGCAAGATGGTAATAACATGAATAAATGGTTTTTCAGTTGGGATGAGAACTCTCTTCTACTTCAACTCAAAGATGGGTTAGGAGATGATGATACTGATGTTATTTATGTTGGATGTCTTAAGGAAGATGTTCATCCAAATGAGCAAGATGAGGTTTCACATATACTTCTTGAGACTTTTAAATGTGTTCCTACTTTTCTCCCAGGTGATCTTTTTACAAGGTATTATCATGGCTTTTGCAAGCAACAGTTATGGCCACTGTTCCATTACATGTTGCCTCTGTCACCAGGCCTTGGTGGTAGGTTCAATAGGTCACTGTGGCAGGCTTATGTATCTGTCAATAAGATATTTGCTGATAGGATTATGGAGGTGATTAACCCTGAAGATGATTATGTTTGGATACATGATTATCATCTCATGGTGTTACCAACTTTCTTGAGGAAGAGGTTTAATAGAGTGAAACTTGGTTTCTTTCTCCACAGCCCATTTCCTTCATCAGAGATTTACAAGACATTGCCTATTAGGGAAGAGCTTTTGAGAGCCCTTTTGAATTCTGATTTGATTGGTTTCCACACTTTTGATTATGCAAGACATTTCCTATCATGTTGTAGTAGGATGCTTGGTCTATCCTATGAATCCAAAAGGGGTTATATTGGGATTGAGTATTATGGTAGGACAGTTAGTATCAAAATTCTTCCTGTTGGCATACACATGGGTCAGCTTCAATCAGTTCTAAGCCTACCAAAGACTGAAGAGAAAGTTTCTGAGCTCATACAACATTTTTCTGATAAAGGAAAGATAATGTTACTTGGTGTGGATGACACAGATATCTTTAAGGGGATAAGTTTGAAGTTGTTAGCCATGGAGCAGCTTCTTATTCAGCACCCTGAGTGGCATGAAAAGGTTGTATTGGTTCAGATAGCTAATCCTGCACGAGGGAAAGGGAAGGATGTGAAGGAAGTGCAAGCCGAGACAAAAGCTACGGCGAAGAGGATTAATGAAAGATTTGGAAAGCCTGGTTATGATCCTGTCATTCTGATTGAGGAGCCATTGAGGTTTTATGAAAAAGTTGCATACTACGTTGTAGCTGAGTGTTGTTTGGTGACTGCAGTTAGGGATGGAATGAATCTTATACCTTATGAATATATAATCAGTCGTCAAGGAAATGAAAAATTGGACAAAGTTTTGGGTATAGGTGCTTCCTCTAAGAAAAGCATGTTGGTTGTGTCTGAGTTTATTGGTTGTTCACCATCTTTAAGTGGAGCTATCAGAGTGAATCCATGGAATATTGATGCAGTAGCTGATGCAATGGACTCAGCTTTGGAAATGGCTGATTCAGAGAAACAGCTTAGGCATGAGAAGCATTACAGATATGTTAGTACTCATGATGTCGGTTACTGGGCCCGTAGCTTCTTGCAAGATTTGGAAAGGATTTGTAGTGATCATGTAAGGAGAAGGTGGTGGGGAATTGGTTTTGGATTAAGTTTTAGAGTTGTAGCACTTGATCCAAACTTCAAAAAGCTTTCAATGGAACACATTGTTTCTGCTTACAAGCAGACGAAAACTAGGGCGATCCTTCTAGATTACGATGGCACTCTAATGCCTCAGGCTTCCATTGATAAGGGTCCAATAAGTAATTCCATTAAAATGCTTAATAGTTTGTGTAGAGATGACAACAACATGGTGTTTATAGTAAGTGCTAAAAGCCGAAAGGCGCTTACTGAATGGTTTTCTCCTTGCGAAAACCTGGGGATTGCTGCTGAGCATGGTTACTTTTTAAGGTAAATTATTTGGGGCTTATGACATTTTCAGGATTTGGATTGCTTGCAATGACAAATCTCATGCAATCATCGCATCGGTGACTGTTAGATGAAAGATCGGACGGTCTAAATTTCAACCTCGGTAGTTTAGAGTTTAAAAATATATATATTATGCACCGTCCGATCTCAATTTAACAGCTATTGATGTGCCGACTATGTGAATTTGCAAACCAGATACACCTTTTCATGGTTTGATACTTCTGTTAGATTATAACTTACAGTTGGCTTGTATGGTTTCAGACTGAAGCGGGATGCAGAATGGGAAACTTGTGCACCTGTAGCAGACTTCAGTTGGAAACAAATTGCAGAGCCTGTGATGAAGCTTTACACCGAAACAACAGATGGATCCACGATTGAAGATAAGGAGACAGCACTTGTTTGGTGCTACGAGGATGCAGATCCTGATTTTGGATCATGCCAAGCTAAAGAACTTCTTAATCATCTTGAAAGTGTGCTTACAAATGAACCAGTAACAGTGAAGAGTGGCTTGAATAATGTGGAGGTTAAACCACAGGTGCGTATAACATTTGTTGAATGAGTTGTAGTGATGACTAATGATAAATTTTCTGTCTATTGGATTCTGGTATACTTAAAAATCTTGTAAATTTAACAAACTTTCGTCACATTAAGACATCATTTTTTCTACAAAAAGAAAATCATAAATATATGAAAACAAAGTTCCTTTACTGTGGTTAGACTTACGATGAAGATACAGCAGGGCAAAAATGAGTTCGGATGGTGTTGTGAGCGCTGTGAAATTCATGTTTAATATTATGTCAAGGGAAAAGTTGAGATGTATTAAAAATCTTTGATAGATGGAAGCATGTAACTTAGGGTTGCATTTGGAAAAGCTTGTTTAAGTTTTATCATGTGACTTTTGCACTTGCATTATGTTTGAAAGAGCTTACAAAAATAGCATATGCAGAGTTTATCTCAGCGTCCCCTAGTTCAATCTTACACTGGCTTTTAGTTGTTAATCTTGTTTGTGTTACCTTGATTCAATCTTTTCCTTTTAGTAAAAGAATGAATATAAACTATAAAGGTAACTTAGGGAATAGCCATTACAGAAAGTGATTATATATAAAATGCATTCTTTTTTTAGTCTTCTTTCCTTGTTGTGTTTGAGAAATGATAAGCTTGGATATTAACCAACCATCTTACAGTCTTACAGCCTCTAATGCATATTCATATACATATATATACACATGCAGGGTGTGAACAAGGGACTTGTGGCTAAGTGCCTACTTTCGGCCATGCAAGAAAAGGGAATGTCACCTGAATTTGTTCTGTGTATTGGAGACGACAGATCTGATGAAGACATGTTTGAGGTCATCACTAGTTCTGGTCCTTCCATGGCTCCTAGGGCTGAAGTATTTGCATGCACTGTTGGTCGAAAACCCAGCAAGGCCAAGTACTACTTGGATGACACTGCCGATATAGTGAGAATGGTGCAGGGTTTAGCCTGTGTTTCAGACCAAATAGTTTTATCTTAG
Protein sequence

>MS.gene62674.t1

MVSRSYSNLLELVSGESPTFENINRRIPRIMTVAGLISDVDDDPLESGCSDPSSSSVQRERIIIVANQLPIKAQRKQDGNNMNKWFFSWDENSLLLQLKDGLGDDDTDVIYVGCLKEDVHPNEQDEVSHILLETFKCVPTFLPGDLFTRYYHGFCKQQLWPLFHYMLPLSPGLGGRFNRSLWQAYVSVNKIFADRIMEVINPEDDYVWIHDYHLMVLPTFLRKRFNRVKLGFFLHSPFPSSEIYKTLPIREELLRALLNSDLIGFHTFDYARHFLSCCSRMLGLSYESKRGYIGIEYYGRTVSIKILPVGIHMGQLQSVLSLPKTEEKVSELIQHFSDKGKIMLLGVDDTDIFKGISLKLLAMEQLLIQHPEWHEKVVLVQIANPARGKGKDVKEVQAETKATAKRINERFGKPGYDPVILIEEPLRFYEKVAYYVVAECCLVTAVRDGMNLIPYEYIISRQGNEKLDKVLGIGASSKKSMLVVSEFIGCSPSLSGAIRVNPWNIDAVADAMDSALEMADSEKQLRHEKHYRYVSTHDVGYWARSFLQDLERICSDHVRRRWWGIGFGLSFRVVALDPNFKKLSMEHIVSAYKQTKTRAILLDYDGTLMPQASIDKGPISNSIKMLNSLCRDDNNMVFIVSAKSRKALTEWFSPCENLGIAAEHGYFLRLKRDAEWETCAPVADFSWKQIAEPVMKLYTETTDGSTIEDKETALVWCYEDADPDFGSCQAKELLNHLESVLTNEPVTVKSGLNNVEVKPQGVNKGLVAKCLLSAMQEKGMSPEFVLCIGDDRSDEDMFEVITSSGPSMAPRAEVFACTVGRKPSKAKYYLDDTADIVRMVQGLACVSDQIVLS