Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63811.t1 | RHN57844.1 | 86 | 235 | 32 | 1 | 1 | 235 | 1 | 234 | 1.60E-109 | 406.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63811.t1 | Q9FLP3 | 64.4 | 180 | 64 | 0 | 354 | 533 | 114 | 293 | 3.1e-53 | 211.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63811.t1 | A0A396I0Z7 | 86.0 | 235 | 32 | 1 | 1 | 235 | 1 | 234 | 1.2e-109 | 406.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene63811 | 0.808237 | 3.42E-50 | -1.69E-46 |
| MS.gene049824 | MS.gene63811 | 0.823416 | 1.40E-53 | -1.69E-46 |
| MS.gene049825 | MS.gene63811 | 0.848019 | 8.00E-60 | -1.69E-46 |
| MS.gene049873 | MS.gene63811 | 0.842299 | 2.81E-58 | -1.69E-46 |
| MS.gene050201 | MS.gene63811 | 0.830176 | 3.40E-55 | -1.69E-46 |
| MS.gene050355 | MS.gene63811 | 0.812224 | 4.72E-51 | -1.69E-46 |
| MS.gene050719 | MS.gene63811 | 0.801376 | 9.27E-49 | -1.69E-46 |
| MS.gene050721 | MS.gene63811 | 0.803152 | 4.00E-49 | -1.69E-46 |
| MS.gene051594 | MS.gene63811 | 0.801446 | 8.97E-49 | -1.69E-46 |
| MS.gene051700 | MS.gene63811 | 0.81612 | 6.51E-52 | -1.69E-46 |
| MS.gene052358 | MS.gene63811 | -0.804774 | 1.84E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene63811 | 0.804596 | 2.00E-49 | -1.69E-46 |
| MS.gene052582 | MS.gene63811 | 0.805177 | 1.51E-49 | -1.69E-46 |
| MS.gene052584 | MS.gene63811 | 0.82779 | 1.29E-54 | -1.69E-46 |
| MS.gene053054 | MS.gene63811 | -0.803286 | 3.75E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene63811 | 0.829355 | 5.39E-55 | -1.69E-46 |
| MS.gene053294 | MS.gene63811 | 0.81434 | 1.62E-51 | -1.69E-46 |
| MS.gene05365 | MS.gene63811 | 0.812727 | 3.66E-51 | -1.69E-46 |
| MS.gene055589 | MS.gene63811 | 0.800507 | 1.40E-48 | -1.69E-46 |
| MS.gene055778 | MS.gene63811 | 0.812533 | 4.04E-51 | -1.69E-46 |
| MS.gene055808 | MS.gene63811 | 0.809794 | 1.59E-50 | -1.69E-46 |
| MS.gene056091 | MS.gene63811 | 0.8209 | 5.37E-53 | -1.69E-46 |
| MS.gene057027 | MS.gene63811 | 0.817447 | 3.28E-52 | -1.69E-46 |
| MS.gene058037 | MS.gene63811 | 0.829208 | 5.85E-55 | -1.69E-46 |
| MS.gene058625 | MS.gene63811 | 0.822686 | 2.07E-53 | -1.69E-46 |
| MS.gene058908 | MS.gene63811 | 0.827984 | 1.16E-54 | -1.69E-46 |
| MS.gene058911 | MS.gene63811 | 0.822559 | 2.22E-53 | -1.69E-46 |
| MS.gene05954 | MS.gene63811 | 0.843849 | 1.09E-58 | -1.69E-46 |
| MS.gene059935 | MS.gene63811 | 0.812381 | 4.36E-51 | -1.69E-46 |
| MS.gene060429 | MS.gene63811 | 0.804829 | 1.79E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene63811 | 0.818123 | 2.31E-52 | -1.69E-46 |
| MS.gene060572 | MS.gene63811 | 0.80585 | 1.09E-49 | -1.69E-46 |
| MS.gene061040 | MS.gene63811 | -0.822061 | 2.90E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63811.t1 | MTR_5g093770 | 95.221 | 272 | 12 | 1 | 268 | 538 | 40 | 311 | 1.61e-156 | 449 |
| MS.gene63811.t1 | MTR_5g093740 | 87.168 | 226 | 28 | 1 | 10 | 235 | 301 | 525 | 1.79e-139 | 414 |
| MS.gene63811.t1 | MTR_5g093740 | 46.970 | 198 | 96 | 4 | 14 | 204 | 56 | 251 | 2.70e-49 | 178 |
| MS.gene63811.t1 | MTR_3g054220 | 66.255 | 243 | 57 | 1 | 293 | 535 | 79 | 296 | 5.56e-103 | 312 |
| MS.gene63811.t1 | MTR_6g082340 | 45.455 | 154 | 77 | 2 | 14 | 162 | 52 | 203 | 5.07e-35 | 131 |
| MS.gene63811.t1 | MTR_6g082260 | 42.949 | 156 | 83 | 2 | 11 | 161 | 49 | 203 | 1.64e-34 | 129 |
| MS.gene63811.t1 | MTR_6g082310 | 44.667 | 150 | 77 | 2 | 16 | 160 | 54 | 202 | 7.07e-33 | 125 |
| MS.gene63811.t1 | MTR_3g061070 | 39.333 | 150 | 84 | 2 | 16 | 160 | 85 | 232 | 5.36e-26 | 111 |
| MS.gene63811.t1 | MTR_2g027540 | 39.073 | 151 | 84 | 3 | 16 | 160 | 91 | 239 | 9.70e-23 | 97.8 |
| MS.gene63811.t1 | MTR_2g027540 | 34.932 | 146 | 73 | 3 | 16 | 160 | 91 | 215 | 1.93e-16 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63811.t1 | AT4G26780 | 68.273 | 249 | 64 | 3 | 290 | 533 | 80 | 318 | 3.51e-93 | 288 |
| MS.gene63811.t1 | AT5G55200 | 72.928 | 181 | 49 | 0 | 353 | 533 | 113 | 293 | 4.06e-85 | 266 |
| MS.gene63811.t1 | AT1G55790 | 41.558 | 154 | 83 | 2 | 16 | 164 | 69 | 220 | 8.21e-31 | 124 |
| MS.gene63811.t1 | AT4G26485 | 38.854 | 157 | 86 | 4 | 14 | 162 | 54 | 208 | 1.40e-28 | 113 |
| MS.gene63811.t1 | AT5G56060 | 38.411 | 151 | 76 | 4 | 10 | 150 | 6 | 149 | 1.70e-24 | 100 |
| MS.gene63811.t1 | AT5G56075 | 37.500 | 144 | 75 | 3 | 22 | 162 | 121 | 252 | 4.06e-23 | 99.4 |
| MS.gene63811.t1 | AT5G25030 | 36.424 | 151 | 80 | 4 | 15 | 162 | 56 | 193 | 4.99e-23 | 97.1 |
| MS.gene63811.t1 | AT1G55800 | 37.600 | 125 | 66 | 1 | 82 | 194 | 16 | 140 | 8.82e-17 | 80.5 |
| MS.gene63811.t1 | AT2G11045 | 32.051 | 156 | 74 | 4 | 8 | 162 | 12 | 136 | 4.31e-15 | 72.8 |
Find 98 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCACTTGATGTATCAATTT+TGG | 0.095462 | 5.3:-75800283 | None:intergenic |
| ACTACTTCCCATTTATTAAA+AGG | 0.156322 | 5.3:-75793930 | None:intergenic |
| ACTTGATGTTGCGGATAATT+TGG | 0.165016 | 5.3:+75800227 | MS.gene63811:CDS |
| CGTTTCGACTTCAACATATT+TGG | 0.197420 | 5.3:+75794229 | MS.gene63811:CDS |
| AAGAACCAACATCAAACTTT+TGG | 0.299666 | 5.3:-75794058 | None:intergenic |
| TAAATGGGAAGTAGTGAAAT+TGG | 0.303632 | 5.3:+75793938 | MS.gene63811:CDS |
| AGCACAGCTGCAACAGTGCC+TGG | 0.313047 | 5.3:-75801504 | None:intergenic |
| AAAGAGGATTCGGATCTAAT+TGG | 0.315457 | 5.3:+75794033 | MS.gene63811:CDS |
| AGCAGAGAGAAGCGAGGTTC+GGG | 0.323559 | 5.3:-75796113 | None:intergenic |
| CTTGATGTTGCGGATAATTT+GGG | 0.353085 | 5.3:+75800228 | MS.gene63811:CDS |
| TGCTTCATGATCGTGTTATT+CGG | 0.355131 | 5.3:+75802086 | MS.gene63811:CDS |
| GAAACTCCTGAAGACACTTC+TGG | 0.368193 | 5.3:+75800320 | MS.gene63811:CDS |
| TTCAAGGAAATTCGCTATAC+AGG | 0.393442 | 5.3:+75800114 | MS.gene63811:CDS |
| AAGAGCAACAATAAATTTGA+TGG | 0.393682 | 5.3:+75793492 | MS.gene63811:CDS |
| GGATCAAAGGGTTCATTTGT+AGG | 0.395749 | 5.3:-75801435 | None:intergenic |
| CACCTTCCAGAAGTGTCTTC+AGG | 0.396210 | 5.3:-75800326 | None:intergenic |
| GAGCAGAGAGAAGCGAGGTT+CGG | 0.396337 | 5.3:-75796114 | None:intergenic |
| TCTCCGTACTTACGCTGAAA+TGG | 0.407067 | 5.3:+75800054 | MS.gene63811:CDS |
| TCTTCAGACTTGGCTTGTCC+AGG | 0.407793 | 5.3:-75799821 | None:intergenic |
| AGAGAAAGCTGGTTTGATAT+TGG | 0.414884 | 5.3:+75793962 | MS.gene63811:CDS |
| AATGTAGAAATGAACCTTGT+TGG | 0.417880 | 5.3:-75796206 | None:intergenic |
| GAGAAGCGAGGTTCGGGAAA+TGG | 0.422213 | 5.3:-75796107 | None:intergenic |
| CATCACCCGAAGTTGGTGTT+GGG | 0.427313 | 5.3:+75793837 | MS.gene63811:CDS |
| GAAGATCAAACAGAAGAATC+AGG | 0.432003 | 5.3:+75799839 | MS.gene63811:CDS |
| GACGTGAAGCAGAGAATTCA+AGG | 0.434720 | 5.3:+75800098 | MS.gene63811:CDS |
| GATGAGGGAATGTAATTGAT+TGG | 0.436632 | 5.3:-75796170 | None:intergenic |
| TTCTGTTTGATCTTCAGACT+TGG | 0.446854 | 5.3:-75799831 | None:intergenic |
| ACAGTGAATCGGATCTATCC+AGG | 0.450640 | 5.3:+75799948 | MS.gene63811:CDS |
| CTACTTCCCATTTATTAAAA+GGG | 0.451717 | 5.3:-75793929 | None:intergenic |
| TACGCAAATAAAAGAGGATT+CGG | 0.452936 | 5.3:+75794023 | MS.gene63811:CDS |
| ATCATTGAATGAAGATCATA+TGG | 0.457962 | 5.3:+75794202 | MS.gene63811:CDS |
| GAAGGGAATAATGTGCATAA+TGG | 0.461273 | 5.3:+75799740 | MS.gene63811:CDS |
| TAAATTTGATGGAACTAAAG+AGG | 0.464832 | 5.3:+75793503 | MS.gene63811:CDS |
| CACATTCTCAAACTGTCAAA+AGG | 0.467135 | 5.3:+75799879 | MS.gene63811:CDS |
| GCACAGCTGCAACAGTGCCT+GGG | 0.468538 | 5.3:-75801503 | None:intergenic |
| GTGAAATTGGCAGAGAAAGC+TGG | 0.471686 | 5.3:+75793951 | MS.gene63811:CDS |
| AGAAAACCCAACACCAACTT+CGG | 0.472302 | 5.3:-75793843 | None:intergenic |
| AACACATTCAAATTCTCAAA+AGG | 0.473855 | 5.3:+75794083 | MS.gene63811:CDS |
| GCATCACCCGAAGTTGGTGT+TGG | 0.474874 | 5.3:+75793836 | MS.gene63811:CDS |
| AAAAGAGATTGAGAAAATGC+AGG | 0.485423 | 5.3:+75800024 | MS.gene63811:CDS |
| ATAACAATCCAGCTGAATGA+TGG | 0.497132 | 5.3:+75802143 | MS.gene63811:CDS |
| GCAACAACGGGTGTTGGTAC+TGG | 0.498694 | 5.3:-75793570 | None:intergenic |
| ACAAAGAATGAACCGAGTCT+TGG | 0.498768 | 5.3:-75794265 | None:intergenic |
| TGGAACCAAAAGTTTGATGT+TGG | 0.504024 | 5.3:+75794053 | MS.gene63811:CDS |
| GTTATTCGGCCAGCGGAAGT+TGG | 0.504351 | 5.3:+75802100 | MS.gene63811:CDS |
| ATAATTTGTCAAGCAACAAC+GGG | 0.504719 | 5.3:-75793582 | None:intergenic |
| GAAAACCCAACACCAACTTC+GGG | 0.508541 | 5.3:-75793842 | None:intergenic |
| CACAGCTGCAACAGTGCCTG+GGG | 0.509586 | 5.3:-75801502 | None:intergenic |
| TCTCCATTTCAGCGTAAGTA+CGG | 0.510972 | 5.3:-75800057 | None:intergenic |
| GATCAAAGGGTTCATTTGTA+GGG | 0.515825 | 5.3:-75801434 | None:intergenic |
| TGGTTTGATATTGGTTGAGA+AGG | 0.517079 | 5.3:+75793971 | MS.gene63811:CDS |
| GTTGAAACAGAAGGGAAAGA+AGG | 0.519141 | 5.3:+75799722 | MS.gene63811:CDS |
| GATTAAGCTTGTAGCTGAGA+AGG | 0.524422 | 5.3:+75799979 | MS.gene63811:CDS |
| CCATGTTACGCAAATAAAAG+AGG | 0.525629 | 5.3:+75794017 | MS.gene63811:CDS |
| AAGCTTAATCAAATCATCCC+TGG | 0.527767 | 5.3:-75799966 | None:intergenic |
| TGGGTTACACCAACTTCCGC+TGG | 0.528802 | 5.3:-75802109 | None:intergenic |
| GTCATGCCTATGTGGATCAA+AGG | 0.531278 | 5.3:-75801448 | None:intergenic |
| TTGAAACAGAAGGGAAAGAA+GGG | 0.534188 | 5.3:+75799723 | MS.gene63811:CDS |
| GCAGAGGAAATTGATCAGCC+TGG | 0.537322 | 5.3:+75799803 | MS.gene63811:CDS |
| AGGAAGATGTTAAGTTGTAA+TGG | 0.539279 | 5.3:+75793876 | MS.gene63811:CDS |
| AAATGGAGAATGTCATGGAC+AGG | 0.541642 | 5.3:+75800071 | MS.gene63811:CDS |
| CTCAAAAGGAAGAGATATTG+TGG | 0.543556 | 5.3:+75794097 | MS.gene63811:CDS |
| AAAGAGTTTACTTGATGTTG+CGG | 0.544338 | 5.3:+75800218 | MS.gene63811:CDS |
| AGCTGTGGAAGTGTTGTTGA+GGG | 0.545466 | 5.3:-75796144 | None:intergenic |
| TTCAGATTCAGACAGTGAAT+CGG | 0.546821 | 5.3:+75799937 | MS.gene63811:CDS |
| AATAATTTGTCAAGCAACAA+CGG | 0.546876 | 5.3:-75793583 | None:intergenic |
| TCATGCCTATGTGGATCAAA+GGG | 0.547596 | 5.3:-75801447 | None:intergenic |
| TTTCCCTTCTGTTTCAACTG+AGG | 0.548286 | 5.3:-75799717 | None:intergenic |
| AAGTGTTGTTGAGGGATTCG+CGG | 0.548610 | 5.3:-75796136 | None:intergenic |
| CGCTGAAATGGAGAATGTCA+TGG | 0.553186 | 5.3:+75800066 | MS.gene63811:CDS |
| TGAACCTTGTTGGTAGAATG+AGG | 0.554586 | 5.3:-75796196 | None:intergenic |
| ATCAAGTGACTCCGCTGAAG+CGG | 0.555608 | 5.3:+75800296 | MS.gene63811:CDS |
| TGTCAAGCAACAACGGGTGT+TGG | 0.558206 | 5.3:-75793576 | None:intergenic |
| CATTCCTCATTCTACCAACA+AGG | 0.561352 | 5.3:+75796192 | MS.gene63811:CDS |
| CTCAAACTGTCAAAAGGAGG+AGG | 0.568785 | 5.3:+75799885 | MS.gene63811:CDS |
| AAACTGTCAAAAGGAGGAGG+AGG | 0.571090 | 5.3:+75799888 | MS.gene63811:CDS |
| CACTGTTGCAGCTGTGCTGA+AGG | 0.572042 | 5.3:+75801509 | MS.gene63811:CDS |
| TCAGCCTCAGTTGAAACAGA+AGG | 0.575095 | 5.3:+75799713 | MS.gene63811:intron |
| GTGCAGGCATCACCCGAAGT+TGG | 0.577764 | 5.3:+75793830 | MS.gene63811:intron |
| CAGGAGTTTCACCGCTTCAG+CGG | 0.583821 | 5.3:-75800307 | None:intergenic |
| AAGCTGTGGAAGTGTTGTTG+AGG | 0.585156 | 5.3:-75796145 | None:intergenic |
| TCAAAATACGATCCAAGACT+CGG | 0.591630 | 5.3:+75794253 | MS.gene63811:CDS |
| TAGTCGACCCAAACTCACTT+CGG | 0.594497 | 5.3:+75794147 | MS.gene63811:CDS |
| GCAGTCTTCGAAGTTCCTGA+TGG | 0.602698 | 5.3:+75801471 | MS.gene63811:CDS |
| GGTAGAATGAGGAATGATGA+GGG | 0.603929 | 5.3:-75796185 | None:intergenic |
| CAGCCTCAGTTGAAACAGAA+GGG | 0.605878 | 5.3:+75799714 | MS.gene63811:intron |
| GACTGAGAAACAACTTTCAG+AGG | 0.609291 | 5.3:+75800356 | MS.gene63811:CDS |
| AAGACTGCGTCATGCCTATG+TGG | 0.609345 | 5.3:-75801456 | None:intergenic |
| TACGGTGTGTACTTATCCTG+AGG | 0.609826 | 5.3:-75793628 | None:intergenic |
| GTAATTGATTGGAGAAGCTG+TGG | 0.610688 | 5.3:-75796159 | None:intergenic |
| TGATCGTGTTATTCGGCCAG+CGG | 0.642674 | 5.3:+75802093 | MS.gene63811:CDS |
| CTCCTGAAGACACTTCTGGA+AGG | 0.644196 | 5.3:+75800324 | MS.gene63811:CDS |
| CAGTGAATCGGATCTATCCA+GGG | 0.646702 | 5.3:+75799949 | MS.gene63811:CDS |
| TGGTAGAATGAGGAATGATG+AGG | 0.653665 | 5.3:-75796186 | None:intergenic |
| ATTCTCAAACTGTCAAAAGG+AGG | 0.665800 | 5.3:+75799882 | MS.gene63811:CDS |
| TCGCGGAGCAGAGAGAAGCG+AGG | 0.670556 | 5.3:-75796119 | None:intergenic |
| ATGAACCCTTTGATCCACAT+AGG | 0.670564 | 5.3:+75801442 | MS.gene63811:CDS |
| ACAGCTGCAACAGTGCCTGG+GGG | 0.671502 | 5.3:-75801501 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 75793442 | 75802162 | 75793442 | ID=MS.gene63811 |
| chr5.3 | mRNA | 75793442 | 75802162 | 75793442 | ID=MS.gene63811.t1;Parent=MS.gene63811 |
| chr5.3 | exon | 75793442 | 75793659 | 75793442 | ID=MS.gene63811.t1.exon1;Parent=MS.gene63811.t1 |
| chr5.3 | CDS | 75793442 | 75793659 | 75793442 | ID=cds.MS.gene63811.t1;Parent=MS.gene63811.t1 |
| chr5.3 | exon | 75793836 | 75794321 | 75793836 | ID=MS.gene63811.t1.exon2;Parent=MS.gene63811.t1 |
| chr5.3 | CDS | 75793836 | 75794321 | 75793836 | ID=cds.MS.gene63811.t1;Parent=MS.gene63811.t1 |
| chr5.3 | exon | 75796104 | 75796213 | 75796104 | ID=MS.gene63811.t1.exon3;Parent=MS.gene63811.t1 |
| chr5.3 | CDS | 75796104 | 75796213 | 75796104 | ID=cds.MS.gene63811.t1;Parent=MS.gene63811.t1 |
| chr5.3 | exon | 75799717 | 75800135 | 75799717 | ID=MS.gene63811.t1.exon4;Parent=MS.gene63811.t1 |
| chr5.3 | CDS | 75799717 | 75800135 | 75799717 | ID=cds.MS.gene63811.t1;Parent=MS.gene63811.t1 |
| chr5.3 | exon | 75800210 | 75800377 | 75800210 | ID=MS.gene63811.t1.exon5;Parent=MS.gene63811.t1 |
| chr5.3 | CDS | 75800210 | 75800377 | 75800210 | ID=cds.MS.gene63811.t1;Parent=MS.gene63811.t1 |
| chr5.3 | exon | 75801402 | 75801530 | 75801402 | ID=MS.gene63811.t1.exon6;Parent=MS.gene63811.t1 |
| chr5.3 | CDS | 75801402 | 75801530 | 75801402 | ID=cds.MS.gene63811.t1;Parent=MS.gene63811.t1 |
| chr5.3 | exon | 75802076 | 75802162 | 75802076 | ID=MS.gene63811.t1.exon7;Parent=MS.gene63811.t1 |
| chr5.3 | CDS | 75802076 | 75802162 | 75802076 | ID=cds.MS.gene63811.t1;Parent=MS.gene63811.t1 |
| Gene Sequence |
| Protein sequence |
