Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66039.t1 | XP_013448803.1 | 98.8 | 81 | 1 | 0 | 6 | 86 | 94 | 174 | 3.90E-34 | 153.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66039.t1 | A0A072UAI2 | 98.8 | 81 | 1 | 0 | 6 | 86 | 94 | 174 | 2.8e-34 | 153.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050397 | MS.gene66039 | 0.810809 | 9.58E-51 | -1.69E-46 |
| MS.gene050426 | MS.gene66039 | -0.812865 | 3.42E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene66039 | 0.835309 | 1.81E-56 | -1.69E-46 |
| MS.gene050529 | MS.gene66039 | 0.834195 | 3.45E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene66039 | 0.834345 | 3.16E-56 | -1.69E-46 |
| MS.gene050971 | MS.gene66039 | 0.810978 | 8.81E-51 | -1.69E-46 |
| MS.gene05105 | MS.gene66039 | 0.824766 | 6.76E-54 | -1.69E-46 |
| MS.gene051525 | MS.gene66039 | 0.816507 | 5.34E-52 | -1.69E-46 |
| MS.gene052011 | MS.gene66039 | -0.808606 | 2.85E-50 | -1.69E-46 |
| MS.gene052078 | MS.gene66039 | 0.814446 | 1.53E-51 | -1.69E-46 |
| MS.gene052188 | MS.gene66039 | 0.817245 | 3.64E-52 | -1.69E-46 |
| MS.gene052253 | MS.gene66039 | 0.85064 | 1.49E-60 | -1.69E-46 |
| MS.gene052310 | MS.gene66039 | 0.814491 | 1.50E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene66039 | 0.811056 | 8.47E-51 | -1.69E-46 |
| MS.gene053449 | MS.gene66039 | 0.801671 | 8.07E-49 | -1.69E-46 |
| MS.gene054349 | MS.gene66039 | 0.803365 | 3.61E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene66039 | 0.820507 | 6.61E-53 | -1.69E-46 |
| MS.gene056511 | MS.gene66039 | 0.800959 | 1.13E-48 | -1.69E-46 |
| MS.gene056899 | MS.gene66039 | -0.824933 | 6.17E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene66039 | 0.805265 | 1.45E-49 | -1.69E-46 |
| MS.gene057749 | MS.gene66039 | 0.809478 | 1.85E-50 | -1.69E-46 |
| MS.gene057823 | MS.gene66039 | 0.818795 | 1.63E-52 | -1.69E-46 |
| MS.gene05811 | MS.gene66039 | 0.825534 | 4.45E-54 | -1.69E-46 |
| MS.gene058276 | MS.gene66039 | 0.802473 | 5.52E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene66039 | 0.801269 | 9.75E-49 | -1.69E-46 |
| MS.gene05886 | MS.gene66039 | 0.800989 | 1.11E-48 | -1.69E-46 |
| MS.gene058928 | MS.gene66039 | 0.828177 | 1.04E-54 | -1.69E-46 |
| MS.gene05907 | MS.gene66039 | 0.824787 | 6.68E-54 | -1.69E-46 |
| MS.gene059420 | MS.gene66039 | 0.822432 | 2.38E-53 | -1.69E-46 |
| MS.gene059688 | MS.gene66039 | 0.815599 | 8.51E-52 | -1.69E-46 |
| MS.gene059692 | MS.gene66039 | 0.816289 | 5.97E-52 | -1.69E-46 |
| MS.gene059695 | MS.gene66039 | 0.820706 | 5.95E-53 | -1.69E-46 |
| MS.gene05984 | MS.gene66039 | 0.813743 | 2.19E-51 | -1.69E-46 |
| MS.gene060403 | MS.gene66039 | 0.822429 | 2.38E-53 | -1.69E-46 |
| MS.gene061049 | MS.gene66039 | 0.823366 | 1.44E-53 | -1.69E-46 |
| MS.gene061154 | MS.gene66039 | 0.837912 | 3.93E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66039.t1 | MTR_7g059255 | 98.765 | 81 | 1 | 0 | 6 | 86 | 94 | 174 | 3.03e-53 | 163 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66039.t1 | AT4G13220 | 60.976 | 82 | 30 | 1 | 7 | 86 | 95 | 176 | 4.64e-29 | 102 |
Find 24 sgRNAs with CRISPR-Local
Find 82 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTACAGCTTTCTTTATTT+TGG | 0.099548 | 7.3:+45552534 | MS.gene66039:CDS |
| GTTACTGAAATCCTTGCTAT+TGG | 0.328579 | 7.3:-45552466 | None:intergenic |
| ATCCTTTGGAAGAGGCTAAT+AGG | 0.388720 | 7.3:+45553382 | MS.gene66039:CDS |
| CTTGATCTTTATTGAATGCT+AGG | 0.393502 | 7.3:-45553314 | None:intergenic |
| GAAATCCTTGCTATTGGTCT+TGG | 0.422289 | 7.3:-45552460 | None:intergenic |
| CAAAGGATCATCCATAGGTT+TGG | 0.442636 | 7.3:-45553367 | None:intergenic |
| CAAACCTATGGATGATCCTT+TGG | 0.442745 | 7.3:+45553368 | MS.gene66039:CDS |
| GGAAGAGGCTAATAGGATAA+TGG | 0.446378 | 7.3:+45553389 | MS.gene66039:CDS |
| CAAGAACCACCTAGCGGGAA+TGG | 0.467436 | 7.3:+45553333 | MS.gene66039:CDS |
| GTTTGTTCATAGGCTACCAT+AGG | 0.522734 | 7.3:+45553273 | MS.gene66039:intron |
| CTGCAGAATGCATGGAGAGT+TGG | 0.536539 | 7.3:+45552093 | None:intergenic |
| AGGAACTACGACCAAACCTA+TGG | 0.545163 | 7.3:+45553356 | MS.gene66039:CDS |
| TGCAAAGTATGCAAGTCCTA+TGG | 0.552339 | 7.3:-45553289 | None:intergenic |
| AGATAGTTCTGCAGAATGCA+TGG | 0.555735 | 7.3:+45552085 | None:intergenic |
| AAGATCAAGAACCACCTAGC+GGG | 0.560815 | 7.3:+45553328 | MS.gene66039:CDS |
| ATCCTATTAGCCTCTTCCAA+AGG | 0.564567 | 7.3:-45553384 | None:intergenic |
| TATGGATGATCCTTTGGAAG+AGG | 0.564870 | 7.3:+45553374 | MS.gene66039:CDS |
| GTAGTTCCTCCATTCCCGCT+AGG | 0.572693 | 7.3:-45553342 | None:intergenic |
| TCTTCCAAAGGATCATCCAT+AGG | 0.581655 | 7.3:-45553372 | None:intergenic |
| AAATAAAGAAAGCTGTAGAG+AGG | 0.616626 | 7.3:-45552531 | None:intergenic |
| GTTCCTCCATTCCCGCTAGG+TGG | 0.621366 | 7.3:-45553339 | None:intergenic |
| GAACCACCTAGCGGGAATGG+AGG | 0.633606 | 7.3:+45553336 | MS.gene66039:CDS |
| CTGTACCAAGACCAATAGCA+AGG | 0.633937 | 7.3:+45552455 | MS.gene66039:CDS |
| AAAGATCAAGAACCACCTAG+CGG | 0.725332 | 7.3:+45553327 | MS.gene66039:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTAATATGATATGAAAAA+TGG | - | chr7.3:45552568-45552587 | None:intergenic | 10.0% |
| !! | AATAGTTGAGACTTATTTAT+AGG | - | chr7.3:45552727-45552746 | None:intergenic | 20.0% |
| !! | ATAGTTGAGACTTATTTATA+GGG | - | chr7.3:45552726-45552745 | None:intergenic | 20.0% |
| !! | ATATATACTGTTATGAATCA+TGG | - | chr7.3:45552873-45552892 | None:intergenic | 20.0% |
| !! | TTCCCTTTAATAACATAATA+TGG | - | chr7.3:45552934-45552953 | None:intergenic | 20.0% |
| ! | AAAAAATTCAACCCTACAAA+AGG | - | chr7.3:45552986-45553005 | None:intergenic | 25.0% |
| ! | AAAAATTCAACCCTACAAAA+GGG | - | chr7.3:45552985-45553004 | None:intergenic | 25.0% |
| ! | ATAGTGAATAAACCATAGTT+AGG | + | chr7.3:45552638-45552657 | MS.gene66039:intron | 25.0% |
| ! | GGCCATATTATGTTATTAAA+GGG | + | chr7.3:45552929-45552948 | MS.gene66039:intron | 25.0% |
| ! | GTAAAAAACTTGAAAACAAC+AGG | - | chr7.3:45552366-45552385 | None:intergenic | 25.0% |
| ! | GTTAGGTTATATGTAAACTA+TGG | + | chr7.3:45552655-45552674 | MS.gene66039:intron | 25.0% |
| ! | GTTTACATATAACCTAACTA+TGG | - | chr7.3:45552653-45552672 | None:intergenic | 25.0% |
| ! | TAGTTGAGACTTATTTATAG+GGG | - | chr7.3:45552725-45552744 | None:intergenic | 25.0% |
| ! | TGGCCATATTATGTTATTAA+AGG | + | chr7.3:45552928-45552947 | MS.gene66039:intron | 25.0% |
| ! | TTGTACTAAATCACTTATTG+CGG | - | chr7.3:45552243-45552262 | None:intergenic | 25.0% |
| !! | GCTAATTATGCTATATTTTG+TGG | + | chr7.3:45553234-45553253 | MS.gene66039:intron | 25.0% |
| !!! | TACAAAGTATTGTCAAACAA+CGG | + | chr7.3:45553088-45553107 | MS.gene66039:intron | 25.0% |
| AAATAAAGAAAGCTGTAGAG+AGG | - | chr7.3:45552534-45552553 | None:intergenic | 30.0% | |
| AAATGGTAATTGTGACTTAC+AGG | - | chr7.3:45552153-45552172 | None:intergenic | 30.0% | |
| AATGGTAATTGTGACTTACA+GGG | - | chr7.3:45552152-45552171 | None:intergenic | 30.0% | |
| ATATTTGTGCTATATGGTAG+CGG | + | chr7.3:45552194-45552213 | MS.gene66039:intron | 30.0% | |
| ATCCTAAAGTCTTAACCATT+GGG | - | chr7.3:45553191-45553210 | None:intergenic | 30.0% | |
| CAAGTACATTGTTTGTTCAT+AGG | + | chr7.3:45553263-45553282 | MS.gene66039:intron | 30.0% | |
| CTTGATCTTTATTGAATGCT+AGG | - | chr7.3:45553317-45553336 | None:intergenic | 30.0% | |
| GAGAAACATGAAAAACTAGA+AGG | - | chr7.3:45552402-45552421 | None:intergenic | 30.0% | |
| TAAGTCACAATTACCATTTC+CGG | + | chr7.3:45552154-45552173 | MS.gene66039:intron | 30.0% | |
| TAGCAGATATTTGTGCTATA+TGG | + | chr7.3:45552188-45552207 | MS.gene66039:intron | 30.0% | |
| TATCCTAAAGTCTTAACCAT+TGG | - | chr7.3:45553192-45553211 | None:intergenic | 30.0% | |
| ! | AAGGATTTCAGTAACTTTTG+TGG | + | chr7.3:45552474-45552493 | MS.gene66039:CDS | 30.0% |
| ! | ATATGTCTTTTCCCTTTTGT+AGG | + | chr7.3:45552971-45552990 | MS.gene66039:intron | 30.0% |
| ! | CTATGGTTAGTTAGTTAGTT+AGG | + | chr7.3:45552672-45552691 | MS.gene66039:intron | 30.0% |
| ! | TATGTCTTTTCCCTTTTGTA+GGG | + | chr7.3:45552972-45552991 | MS.gene66039:intron | 30.0% |
| !! | CTCTACAGCTTTCTTTATTT+TGG | + | chr7.3:45552534-45552553 | MS.gene66039:CDS | 30.0% |
| AAGTCACAATTACCATTTCC+GGG | + | chr7.3:45552155-45552174 | MS.gene66039:intron | 35.0% | |
| ACATGAATGAACATGGCAAT+TGG | - | chr7.3:45552906-45552925 | None:intergenic | 35.0% | |
| ACTCTACTATGAGTCACTAT+GGG | + | chr7.3:45552780-45552799 | MS.gene66039:intron | 35.0% | |
| ATAGCATAGGTGTATTCATG+TGG | - | chr7.3:45552812-45552831 | None:intergenic | 35.0% | |
| ATCAGCAAGAAAGATAGCAT+AGG | - | chr7.3:45552825-45552844 | None:intergenic | 35.0% | |
| ATCATTATGACCTTTATCCC+TGG | + | chr7.3:45552843-45552862 | MS.gene66039:intron | 35.0% | |
| ATTGACAACAAGTTTCCCAA+TGG | + | chr7.3:45553173-45553192 | MS.gene66039:intron | 35.0% | |
| TACTCTACTATGAGTCACTA+TGG | + | chr7.3:45552779-45552798 | MS.gene66039:intron | 35.0% | |
| TAGCATAGGTGTATTCATGT+GGG | - | chr7.3:45552811-45552830 | None:intergenic | 35.0% | |
| TCATTATGACCTTTATCCCT+GGG | + | chr7.3:45552844-45552863 | MS.gene66039:intron | 35.0% | |
| TCTAGCATCAGCAAAAAGAA+GGG | - | chr7.3:45552432-45552451 | None:intergenic | 35.0% | |
| TTATGAATCATGGAATCCCA+GGG | - | chr7.3:45552863-45552882 | None:intergenic | 35.0% | |
| TTCCCAATGGTTAAGACTTT+AGG | + | chr7.3:45553186-45553205 | MS.gene66039:intron | 35.0% | |
| TTCTAGCATCAGCAAAAAGA+AGG | - | chr7.3:45552433-45552452 | None:intergenic | 35.0% | |
| ! | GTTACTGAAATCCTTGCTAT+TGG | - | chr7.3:45552469-45552488 | None:intergenic | 35.0% |
| ! | TACAAAGTCTTGTCGAATAG+TGG | + | chr7.3:45552258-45552277 | MS.gene66039:intron | 35.0% |
| !! | GGGAAACTTGTTGTCAATTA+CGG | - | chr7.3:45553171-45553190 | None:intergenic | 35.0% |
| AAAGATCAAGAACCACCTAG+CGG | + | chr7.3:45553327-45553346 | MS.gene66039:CDS | 40.0% | |
| ATCACTTATTGCGGAACAAC+AGG | - | chr7.3:45552234-45552253 | None:intergenic | 40.0% | |
| CAAACCTATGGATGATCCTT+TGG | + | chr7.3:45553368-45553387 | MS.gene66039:CDS | 40.0% | |
| CAAAGGATCATCCATAGGTT+TGG | - | chr7.3:45553370-45553389 | None:intergenic | 40.0% | |
| GGAAGAGGCTAATAGGATAA+TGG | + | chr7.3:45553389-45553408 | MS.gene66039:CDS | 40.0% | |
| GTTATGAATCATGGAATCCC+AGG | - | chr7.3:45552864-45552883 | None:intergenic | 40.0% | |
| GTTTGTTCATAGGCTACCAT+AGG | + | chr7.3:45553273-45553292 | MS.gene66039:intron | 40.0% | |
| TATTTGACAATGCCCGGAAA+TGG | - | chr7.3:45552170-45552189 | None:intergenic | 40.0% | |
| TCGAATAGTGGCTATAACAG+TGG | + | chr7.3:45552270-45552289 | MS.gene66039:intron | 40.0% | |
| TCTTCCAAAGGATCATCCAT+AGG | - | chr7.3:45553375-45553394 | None:intergenic | 40.0% | |
| TGCAAAGTATGCAAGTCCTA+TGG | - | chr7.3:45553292-45553311 | None:intergenic | 40.0% | |
| TGCCATGTTCATTCATGTAC+TGG | + | chr7.3:45552908-45552927 | MS.gene66039:intron | 40.0% | |
| ! | ATAGCTGCTGTTTGAAACAG+TGG | - | chr7.3:45553019-45553038 | None:intergenic | 40.0% |
| ! | ATCCTATTAGCCTCTTCCAA+AGG | - | chr7.3:45553387-45553406 | None:intergenic | 40.0% |
| ! | ATCCTTTGGAAGAGGCTAAT+AGG | + | chr7.3:45553382-45553401 | MS.gene66039:CDS | 40.0% |
| ! | ATCTGCTATTTGACAATGCC+CGG | - | chr7.3:45552176-45552195 | None:intergenic | 40.0% |
| ! | TATGGATGATCCTTTGGAAG+AGG | + | chr7.3:45553374-45553393 | MS.gene66039:CDS | 40.0% |
| !! | GAAATCCTTGCTATTGGTCT+TGG | - | chr7.3:45552463-45552482 | None:intergenic | 40.0% |
| !!! | ACAGGCAGTTTTTTCAATCG+CGG | - | chr7.3:45552348-45552367 | None:intergenic | 40.0% |
| AAGATCAAGAACCACCTAGC+GGG | + | chr7.3:45553328-45553347 | MS.gene66039:CDS | 45.0% | |
| AGGAACTACGACCAAACCTA+TGG | + | chr7.3:45553356-45553375 | MS.gene66039:CDS | 45.0% | |
| CAATCGCGGATCACAGAAAA+TGG | - | chr7.3:45552334-45552353 | None:intergenic | 45.0% | |
| CTGTACCAAGACCAATAGCA+AGG | + | chr7.3:45552455-45552474 | MS.gene66039:CDS | 45.0% | |
| GGCCAGTACATGAATGAACA+TGG | - | chr7.3:45552913-45552932 | None:intergenic | 45.0% | |
| GGTAAGGATGGAATTGATGC+AGG | + | chr7.3:45552114-45552133 | MS.gene66039:intron | 45.0% | |
| GTTATAGCACTGCAGTGTAG+CGG | + | chr7.3:45552294-45552313 | MS.gene66039:intron | 45.0% | |
| TCATGGAATCCCAGGGATAA+AGG | - | chr7.3:45552856-45552875 | None:intergenic | 45.0% | |
| !! | GCATGGAGAGTTGGTAAGGA+TGG | + | chr7.3:45552102-45552121 | MS.gene66039:intron | 50.0% |
| CAAGAACCACCTAGCGGGAA+TGG | + | chr7.3:45553333-45553352 | MS.gene66039:CDS | 55.0% | |
| GTAGTTCCTCCATTCCCGCT+AGG | - | chr7.3:45553345-45553364 | None:intergenic | 55.0% | |
| GAACCACCTAGCGGGAATGG+AGG | + | chr7.3:45553336-45553355 | MS.gene66039:CDS | 60.0% | |
| GTTCCTCCATTCCCGCTAGG+TGG | - | chr7.3:45553342-45553361 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 45552100 | 45553425 | 45552100 | ID=MS.gene66039 |
| chr7.3 | mRNA | 45552100 | 45553425 | 45552100 | ID=MS.gene66039.t1;Parent=MS.gene66039 |
| chr7.3 | exon | 45552100 | 45552114 | 45552100 | ID=MS.gene66039.t1.exon1;Parent=MS.gene66039.t1 |
| chr7.3 | CDS | 45552100 | 45552114 | 45552100 | ID=cds.MS.gene66039.t1;Parent=MS.gene66039.t1 |
| chr7.3 | exon | 45552451 | 45552555 | 45552451 | ID=MS.gene66039.t1.exon2;Parent=MS.gene66039.t1 |
| chr7.3 | CDS | 45552451 | 45552555 | 45552451 | ID=cds.MS.gene66039.t1;Parent=MS.gene66039.t1 |
| chr7.3 | exon | 45553285 | 45553425 | 45553285 | ID=MS.gene66039.t1.exon3;Parent=MS.gene66039.t1 |
| chr7.3 | CDS | 45553285 | 45553425 | 45553285 | ID=cds.MS.gene66039.t1;Parent=MS.gene66039.t1 |
| Gene Sequence |
| Protein sequence |
