Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66928.t1 | XP_003589126.1 | 100 | 78 | 0 | 0 | 1 | 78 | 55 | 132 | 8.50E-36 | 159.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66928.t1 | Q8GXG1 | 93.6 | 78 | 5 | 0 | 1 | 78 | 55 | 132 | 4.3e-36 | 151.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66928.t1 | Q2HTR7 | 100.0 | 78 | 0 | 0 | 1 | 78 | 55 | 132 | 6.1e-36 | 159.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene66928 | 0.821549 | 3.81E-53 | -1.69E-46 |
| MS.gene049350 | MS.gene66928 | 0.818492 | 1.91E-52 | -1.69E-46 |
| MS.gene049807 | MS.gene66928 | 0.811133 | 8.16E-51 | -1.69E-46 |
| MS.gene049887 | MS.gene66928 | 0.843757 | 1.15E-58 | -1.69E-46 |
| MS.gene050551 | MS.gene66928 | 0.8049 | 1.73E-49 | -1.69E-46 |
| MS.gene050838 | MS.gene66928 | 0.835797 | 1.36E-56 | -1.69E-46 |
| MS.gene051102 | MS.gene66928 | 0.809131 | 2.20E-50 | -1.69E-46 |
| MS.gene051234 | MS.gene66928 | 0.804288 | 2.32E-49 | -1.69E-46 |
| MS.gene051852 | MS.gene66928 | 0.800314 | 1.53E-48 | -1.69E-46 |
| MS.gene05224 | MS.gene66928 | 0.809625 | 1.73E-50 | -1.69E-46 |
| MS.gene052307 | MS.gene66928 | 0.817848 | 2.67E-52 | -1.69E-46 |
| MS.gene052335 | MS.gene66928 | 0.80759 | 4.69E-50 | -1.69E-46 |
| MS.gene052459 | MS.gene66928 | 0.811164 | 8.03E-51 | -1.69E-46 |
| MS.gene052463 | MS.gene66928 | 0.809938 | 1.48E-50 | -1.69E-46 |
| MS.gene052516 | MS.gene66928 | 0.816461 | 5.46E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene66928 | 0.819741 | 9.90E-53 | -1.69E-46 |
| MS.gene052732 | MS.gene66928 | 0.808581 | 2.89E-50 | -1.69E-46 |
| MS.gene053143 | MS.gene66928 | 0.813403 | 2.60E-51 | -1.69E-46 |
| MS.gene053144 | MS.gene66928 | 0.824935 | 6.16E-54 | -1.69E-46 |
| MS.gene053381 | MS.gene66928 | 0.811596 | 6.47E-51 | -1.69E-46 |
| MS.gene053581 | MS.gene66928 | 0.836224 | 1.06E-56 | -1.69E-46 |
| MS.gene054584 | MS.gene66928 | 0.81694 | 4.27E-52 | -1.69E-46 |
| MS.gene055465 | MS.gene66928 | 0.820616 | 6.24E-53 | -1.69E-46 |
| MS.gene055466 | MS.gene66928 | 0.815382 | 9.51E-52 | -1.69E-46 |
| MS.gene055468 | MS.gene66928 | 0.835269 | 1.85E-56 | -1.69E-46 |
| MS.gene055469 | MS.gene66928 | 0.860918 | 1.48E-63 | -1.69E-46 |
| MS.gene055513 | MS.gene66928 | 0.827147 | 1.84E-54 | -1.69E-46 |
| MS.gene055580 | MS.gene66928 | -0.820581 | 6.36E-53 | -1.69E-46 |
| MS.gene056043 | MS.gene66928 | -0.836846 | 7.37E-57 | -1.69E-46 |
| MS.gene056308 | MS.gene66928 | 0.831159 | 1.95E-55 | -1.69E-46 |
| MS.gene056390 | MS.gene66928 | 0.801853 | 7.40E-49 | -1.69E-46 |
| MS.gene056522 | MS.gene66928 | -0.828401 | 9.18E-55 | -1.69E-46 |
| MS.gene058720 | MS.gene66928 | 0.809331 | 1.99E-50 | -1.69E-46 |
| MS.gene059170 | MS.gene66928 | 0.832624 | 8.50E-56 | -1.69E-46 |
| MS.gene060932 | MS.gene66928 | 0.805869 | 1.08E-49 | -1.69E-46 |
| MS.gene061006 | MS.gene66928 | 0.836562 | 8.70E-57 | -1.69E-46 |
| MS.gene061100 | MS.gene66928 | 0.817904 | 2.59E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66928.t1 | MTR_1g018760 | 100.000 | 78 | 0 | 0 | 1 | 78 | 55 | 132 | 1.48e-51 | 163 |
| MS.gene66928.t1 | MTR_3g102370 | 87.179 | 78 | 10 | 0 | 1 | 78 | 55 | 132 | 2.34e-44 | 145 |
| MS.gene66928.t1 | MTR_1g094030 | 87.179 | 78 | 10 | 0 | 1 | 78 | 55 | 132 | 5.34e-44 | 144 |
| MS.gene66928.t1 | MTR_4g109920 | 78.205 | 78 | 17 | 0 | 1 | 78 | 55 | 132 | 1.52e-37 | 127 |
| MS.gene66928.t1 | MTR_4g109900 | 78.205 | 78 | 17 | 0 | 1 | 78 | 54 | 131 | 2.31e-37 | 127 |
| MS.gene66928.t1 | MTR_8g031580 | 38.636 | 88 | 44 | 1 | 1 | 78 | 70 | 157 | 6.77e-12 | 59.3 |
| MS.gene66928.t1 | MTR_8g031580 | 38.636 | 88 | 44 | 1 | 1 | 78 | 70 | 157 | 7.34e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66928.t1 | AT3G16150 | 93.590 | 78 | 5 | 0 | 1 | 78 | 55 | 132 | 3.48e-48 | 155 |
| MS.gene66928.t1 | AT5G08100 | 74.359 | 78 | 20 | 0 | 1 | 78 | 56 | 133 | 1.89e-35 | 122 |
| MS.gene66928.t1 | AT5G08100 | 77.358 | 53 | 12 | 0 | 26 | 78 | 1 | 53 | 2.69e-23 | 88.6 |
Find 17 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACGATGCGGTGCCGTTTC+AGG | 0.158496 | 1.4:-4394831 | MS.gene66928:CDS |
| GGATCTGCCCTGACGGAAAA+AGG | 0.260928 | 1.4:-4394894 | MS.gene66928:CDS |
| ACTAAACGAGCCAGTGAAAT+TGG | 0.271206 | 1.4:+4394789 | None:intergenic |
| AACGGATCCATTATTCAATT+CGG | 0.328084 | 1.4:-4394922 | MS.gene66928:CDS |
| GTTATTGAAGGTGAGGGAAT+TGG | 0.347604 | 1.4:-4394946 | None:intergenic |
| ACGGATCCATTATTCAATTC+GGG | 0.402987 | 1.4:-4394921 | MS.gene66928:CDS |
| CCACGACCCGAATTGAATAA+TGG | 0.409899 | 1.4:+4394915 | None:intergenic |
| TACTGTGGTTAAACCTGAAA+CGG | 0.417200 | 1.4:+4394818 | None:intergenic |
| CCATTATTCAATTCGGGTCG+TGG | 0.423817 | 1.4:-4394915 | MS.gene66928:CDS |
| GGAAATGGAGGCTAGCATAA+TGG | 0.434511 | 1.4:-4394865 | MS.gene66928:CDS |
| AGCGCCGTTAAAAGCAAGAT+AGG | 0.481695 | 1.4:+4394746 | None:intergenic |
| TTCACTGGCTCGTTTAGTCA+TGG | 0.486469 | 1.4:-4394784 | MS.gene66928:CDS |
| GAAGGTGAGGGAATTGGAAA+CGG | 0.565330 | 1.4:-4394940 | None:intergenic |
| GGGTCGTGGATCTGCCCTGA+CGG | 0.570628 | 1.4:-4394901 | MS.gene66928:CDS |
| ATGGAGGCTAGCATAATGGA+TGG | 0.598729 | 1.4:-4394861 | MS.gene66928:CDS |
| AAAAGGAACGGTGGAAATGG+AGG | 0.622339 | 1.4:-4394877 | MS.gene66928:CDS |
| TTAAAAGCAAGATAGGAATG+AGG | 0.636686 | 1.4:+4394753 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGTAAAAAATCCAATTTCAC+TGG | - | chr1.4:4394862-4394881 | MS.gene66928:CDS | 25.0% |
| !!! | TGAAATTGGATTTTTTACTG+TGG | + | chr1.4:4394861-4394880 | None:intergenic | 25.0% |
| AACGGATCCATTATTCAATT+CGG | - | chr1.4:4394739-4394758 | MS.gene66928:CDS | 30.0% | |
| TTAAAAGCAAGATAGGAATG+AGG | + | chr1.4:4394911-4394930 | None:intergenic | 30.0% | |
| !! | CATTCCTATCTTGCTTTTAA+CGG | - | chr1.4:4394911-4394930 | MS.gene66928:CDS | 30.0% |
| ACGGATCCATTATTCAATTC+GGG | - | chr1.4:4394740-4394759 | MS.gene66928:CDS | 35.0% | |
| TACTGTGGTTAAACCTGAAA+CGG | + | chr1.4:4394846-4394865 | None:intergenic | 35.0% | |
| ! | TGAAGATTTTGCTAGACAAC+AGG | - | chr1.4:4394937-4394956 | MS.gene66928:CDS | 35.0% |
| ACTAAACGAGCCAGTGAAAT+TGG | + | chr1.4:4394875-4394894 | None:intergenic | 40.0% | |
| AAAAGGAACGGTGGAAATGG+AGG | - | chr1.4:4394784-4394803 | MS.gene66928:CDS | 45.0% | |
| AGCGCCGTTAAAAGCAAGAT+AGG | + | chr1.4:4394918-4394937 | None:intergenic | 45.0% | |
| CCACGACCCGAATTGAATAA+TGG | + | chr1.4:4394749-4394768 | None:intergenic | 45.0% | |
| CCATTATTCAATTCGGGTCG+TGG | - | chr1.4:4394746-4394765 | MS.gene66928:CDS | 45.0% | |
| GATGGACCAAAAAGACGATG+CGG | - | chr1.4:4394818-4394837 | MS.gene66928:CDS | 45.0% | |
| GGAAAAAGGAACGGTGGAAA+TGG | - | chr1.4:4394781-4394800 | MS.gene66928:CDS | 45.0% | |
| GGAAATGGAGGCTAGCATAA+TGG | - | chr1.4:4394796-4394815 | MS.gene66928:CDS | 45.0% | |
| ! | ATGGAGGCTAGCATAATGGA+TGG | - | chr1.4:4394800-4394819 | MS.gene66928:CDS | 45.0% |
| !! | TTCACTGGCTCGTTTAGTCA+TGG | - | chr1.4:4394877-4394896 | MS.gene66928:CDS | 45.0% |
| TGCCCTGACGGAAAAAGGAA+CGG | - | chr1.4:4394772-4394791 | MS.gene66928:CDS | 50.0% | |
| ! | CACCGTTCCTTTTTCCGTCA+GGG | + | chr1.4:4394777-4394796 | None:intergenic | 50.0% |
| CCTGACGGAAAAAGGAACGG+TGG | - | chr1.4:4394775-4394794 | MS.gene66928:CDS | 55.0% | |
| GGATCTGCCCTGACGGAAAA+AGG | - | chr1.4:4394767-4394786 | MS.gene66928:CDS | 55.0% | |
| ! | ACGGCACCGCATCGTCTTTT+TGG | + | chr1.4:4394827-4394846 | None:intergenic | 55.0% |
| ! | CCACCGTTCCTTTTTCCGTC+AGG | + | chr1.4:4394778-4394797 | None:intergenic | 55.0% |
| !! | AGACGATGCGGTGCCGTTTC+AGG | - | chr1.4:4394830-4394849 | MS.gene66928:CDS | 60.0% |
| GGGTCGTGGATCTGCCCTGA+CGG | - | chr1.4:4394760-4394779 | MS.gene66928:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 4394725 | 4394958 | 4394725 | ID=MS.gene66928 |
| chr1.4 | mRNA | 4394725 | 4394958 | 4394725 | ID=MS.gene66928.t1;Parent=MS.gene66928 |
| chr1.4 | exon | 4394725 | 4394958 | 4394725 | ID=MS.gene66928.t1.exon1;Parent=MS.gene66928.t1 |
| chr1.4 | CDS | 4394725 | 4394958 | 4394725 | ID=cds.MS.gene66928.t1;Parent=MS.gene66928.t1 |
| Gene Sequence |
| Protein sequence |
