AlfalfaGEDB | MS.gene77418

M. sativa cultivar XinJiangDaYe / MS.gene77418

KEGG: K04713
GO: GO:0003674 GO:0003824 GO:0005488 GO:0005506 GO:0006629 GO:0008150 GO:0008152 GO:0008610 GO:0009058 GO:0016491 GO:0043167 GO:0043169 GO:0044238 GO:0046872 GO:0046914 GO:0055114 GO:0071704 GO:1901576
PFAM: PF04116.16

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77418.t1	XP_003613744.1	97.7	263	6	0	1	263	1	263	1.30E-144	522.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77418.t1	Q8VYI1	77.4	261	58	1	3	263	1	260	7.4e-117	421.4

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77418.t1	B7FLU5	97.7	263	6	0	1	263	1	263	9.3e-145	522.3

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene77418	0.811371	7.24E-51	-1.69E-46
MS.gene051043	MS.gene77418	0.836172	1.09E-56	-1.69E-46
MS.gene052118	MS.gene77418	0.801795	7.61E-49	-1.69E-46
MS.gene054489	MS.gene77418	0.823804	1.14E-53	-1.69E-46
MS.gene054892	MS.gene77418	0.813531	2.44E-51	-1.69E-46
MS.gene054893	MS.gene77418	0.811676	6.21E-51	-1.69E-46
MS.gene055229	MS.gene77418	0.815069	1.12E-51	-1.69E-46
MS.gene056677	MS.gene77418	0.804443	2.16E-49	-1.69E-46
MS.gene058279	MS.gene77418	0.821682	3.55E-53	-1.69E-46
MS.gene05952	MS.gene77418	0.810461	1.14E-50	-1.69E-46

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PPI:

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Gene1	Gene2	Type
MS.gene003352	MS.gene77418	PPI
MS.gene02144	MS.gene77418	PPI
MS.gene023338	MS.gene77418	PPI
MS.gene032882	MS.gene77418	PPI
MS.gene08289	MS.gene77418	PPI
MS.gene34901	MS.gene77418	PPI
MS.gene55207	MS.gene77418	PPI
MS.gene68324	MS.gene77418	PPI
MS.gene77418	MS.gene027563	PPI
MS.gene77418	MS.gene02757	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77418.t1	MTR_5g040320	97.719	263	6	0	1	263	1	263	0.0	535
MS.gene77418.t1	MTR_1g097310	67.200	250	79	2	9	258	8	254	2.32e-125	357
MS.gene77418.t1	MTR_1g097320	63.704	135	49	0	76	210	1	135	1.07e-60	188
MS.gene77418.t1	MTR_6g039970	27.536	207	132	8	39	238	52	247	1.79e-13	68.9
MS.gene77418.t1	MTR_8g063210	27.488	211	135	8	39	242	52	251	2.47e-13	68.6
MS.gene77418.t1	MTR_5g035190	28.251	223	141	9	39	253	52	263	5.60e-13	67.4
MS.gene77418.t1	MTR_5g035190	29.557	203	127	8	59	253	15	209	7.32e-13	66.2
MS.gene77418.t1	MTR_4g102060	31.788	151	94	4	101	247	129	274	9.36e-11	61.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene77418.t1	AT1G14290	77.255	255	57	1	9	263	6	259	4.91e-153	427
MS.gene77418.t1	AT1G69640	77.395	261	58	1	3	263	1	260	2.42e-144	405
MS.gene77418.t1	AT1G14290	82.065	184	33	0	80	263	2	185	8.87e-116	330
MS.gene77418.t1	AT2G29390	26.818	220	135	8	36	238	49	259	3.00e-12	65.5

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Find 56 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCGTGGATGATCACTG+TGG	0.771335	5.1:+30433589	MS.gene77418:CDS
AAAACTTACTGCAAACAAAA+GGG	0.533273	5.1:-30432004	None:intergenic
AACATGTGGAATAGGTTCCC+AGG	0.518779	5.1:-30433622	None:intergenic
AAGGAGAGAAGTATTCTCAT+TGG	0.524581	5.1:-30433302	None:intergenic
AATGACGATGGATTTGGATT+TGG	0.283990	5.1:+30431780	None:intergenic
ACAATTTATTACTGCAATGT+TGG	0.447512	5.1:+30433336	MS.gene77418:CDS
ACAGTTGATGGTGTACGTCG+TGG	0.672465	5.1:-30433666	None:intergenic
ACATGTGGAATAGGTTCCCA+GGG	0.610107	5.1:-30433621	None:intergenic
ACTGTGGTTTATGGCTCCCT+GGG	0.512563	5.1:+30433605	MS.gene77418:CDS
ACTTGTTGCCATACAGTTGA+TGG	0.408130	5.1:-30433678	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AACTATAACTAAACTGTGGT+TGG	+	chr5.1:30433233-30433252	MS.gene77418:intron	30.0%
AATGAAGTTGTGATTGTATC+TGG	+	chr5.1:30433202-30433221	MS.gene77418:intron	30.0%
ACTATAACTAAACTGTGGTT+GGG	+	chr5.1:30433234-30433253	MS.gene77418:intron	30.0%
AGATGAGGATGAAAAGAATT+TGG	+	chr5.1:30431918-30431937	MS.gene77418:CDS	30.0%
ATATAACACAATTGCAGACT+TGG	+	chr5.1:30432712-30432731	MS.gene77418:intron	30.0%
CAGAATCTACAACAGTAAAA+AGG	-	chr5.1:30432456-30432475	None:intergenic	30.0%
CCTTAAAAGTCAACACATAA+AGG	-	chr5.1:30432288-30432307	None:intergenic	30.0%
GAGAGTGAATATGTTTGTAT+AGG	-	chr5.1:30433414-30433433	None:intergenic	30.0%
GTTACATACAAAGAAAGATG+AGG	+	chr5.1:30431903-30431922	MS.gene77418:CDS	30.0%
TACTAGTAATTCATAAGTCC+TGG	-	chr5.1:30432184-30432203	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	30431781	30433828	30431781	ID=MS.gene77418
chr5.1	mRNA	30431781	30433828	30431781	ID=MS.gene77418.t1;Parent=MS.gene77418
chr5.1	exon	30431781	30432017	30431781	ID=MS.gene77418.t1.exon1;Parent=MS.gene77418.t1
chr5.1	CDS	30431781	30432017	30431781	ID=cds.MS.gene77418.t1;Parent=MS.gene77418.t1
chr5.1	exon	30433274	30433828	30433274	ID=MS.gene77418.t1.exon2;Parent=MS.gene77418.t1
chr5.1	CDS	30433274	30433828	30433274	ID=cds.MS.gene77418.t1;Parent=MS.gene77418.t1

Gene Sequence

>MS.gene77418

ATGACGATGGATTTGGATTTGGGGGTTTCTGATGAGATGTTAGGGACTTTTGCTCCACTTTTAGTTTATTGGATTTATTCTGGTTTTTATGTTGTTTTGGGATTGTTTGCTGAAGATTATAGGTTACATACAAAGAAAGATGAGGATGAAAAGAATTTGGTTTCTAAGTTTGATGTTGTTAAAGGTGTTCTTCTTCAACAAGCAGTTCAAGCTGTTGTTGCAACCCTTTTGTTTGCAGTAAGTTTTTTGTGATGGAAAGATTTTACTAGTAGATGTTTTTTGTTTGTTTGTAGAGGGATTGGTAAATACCACATGAAACTCGAACAAATGAGAGGTCCTGGCTTCGAATTCGGGACACAACATATATTCGGCTTAACAATTTTAGCATTTGCCAGTTGAGCCAGGACTTATGAATTACTAGTAGATATAATAATTGAGTTGATTTTATATTGCCTAAGCTGGTTTATGGAGGTTTAATTGCTTAATGAATTGATCTATTTGGTCCCTTTATGTGTTGACTTTTAAGGAAAAGTAGACAACAAACAATTTTGTTGTCAATATCTTGCGTTTTGTCTTTTTTTATCATAGTTTACCCTCTTTTGCAAATACTATGTTTTGCCTAGTTGTTTAGAGTTCAGAGCTAAATAGGGTTGACTTTGTGTATGATGTTTTCCTTTTTACTGTTGTAGATTCTGATAAGGTTTTGTTTCTATTTGGTATATGTTACTTAATTAATGAATCTCTTGAGTTGGAGTATTTCAATATGTATAGCTGATTGAATTGTTTTTGAGCCTGTGTTTATCTTAGGCTACATCAAAATGGAGAGTTGTATTGCATCTGTAGATACTATAGTGGGTTATAGGAACTCCTTAATTGTTATATGTTATACACACAAAATAAGTTTCTTTTACGCAAGAAAGGAAAGAAGAGCATATAACACAATTGCAGACTTGGGTTCTAGTGGTTATGAATTGTTCTTCCGTTTCAAAAATTAGAAAGATAAATTGTGAACCGTTGCAAACATTGACTTGTCTCGATCAGTTTAGAGGTTTTGCATTTACTATCTATGAAGCGTTTTCGAGCCAAATCTTGTATGGATTTTACTTTGGAGTCGTTGAGTTAATATCACGAGTGTTGTCAAATAGCGGCTATAGCATATCAGAATTTGAAAATATCGCTATTATTCTGCAATACTTTATTTACAACTAAGTGTTCTCAAATAGCAGCTATATCATAGAGATTTTGAACAACGCTATATAATATGTACTTTTTGTGTTTATCATGTTGAATTATTGTGAAGTAATTAAGTTGCAATTGTATCTGGATGACTAATTGAGAAAACCACTATTTTCAATGATCCGGGTTGACAATGCAGTATCAACTGTACTTTTTGTGCTTACTATGTTGAATTATTGTGAAGTAATGAAGTTGTGATTGTATCTGGATAACTATAACTATAACTAAACTGTGGTTGGGTTTTTGTTTTTGTGTAGGTGACAGGAAGTGATAGCCAAAGTAGTACCAATGAGAATACTTCTCTCCTTGTTTTAGCAAGACAATTTATTACTGCAATGTTGGTTATGGACACATGGCAATATTTTATGCACAGATACATGCATCAGAACAAGTTCCTATACAAACATATTCACTCTCTACATCACAGGCTCATTGTACCCTATTCATTTGGAGCTCTATACAATCATCCCATCGAGGGGCTTCTTAACGACACAATCGGCGGAGCTTTATCTTTTCTCCTATCTGGTATGAGTCCTCGAGCATCCATTTTTTTCTTCTCCTTTGCCACCATTAAAACCGTGGATGATCACTGTGGTTTATGGCTCCCTGGGAACCTATTCCACATGTTTTTCAATAACAATTCTGCTTACCACGACGTACACCATCAACTGTATGGCAACAAGTACAACTTCTCGCAACCGTTCTTTGTTATGTGGGACAAAATCTTAGGTACCTACATGCCATACTCATTAGAGAAGAGAGCTAGTGGCGGTTTTGAATCTAGACCTTGTAAACCTTACAAGGATGAATGA

Protein sequence

>MS.gene77418.t1

MTMDLDLGVSDEMLGTFAPLLVYWIYSGFYVVLGLFAEDYRLHTKKDEDEKNLVSKFDVVKGVLLQQAVQAVVATLLFAVTGSDSQSSTNENTSLLVLARQFITAMLVMDTWQYFMHRYMHQNKFLYKHIHSLHHRLIVPYSFGALYNHPIEGLLNDTIGGALSFLLSGMSPRASIFFFSFATIKTVDDHCGLWLPGNLFHMFFNNNSAYHDVHHQLYGNKYNFSQPFFVMWDKILGTYMPYSLEKRASGGFESRPCKPYKDE