Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69400.t1 | XP_003603759.1 | 95.1 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.80E-49 | 204.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69400.t1 | P46689 | 58.3 | 103 | 38 | 1 | 1 | 103 | 1 | 98 | 7.4e-28 | 124.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69400.t1 | G7J582 | 95.1 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.3e-49 | 204.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene69400 | 0.828069 | 1.10E-54 | -1.69E-46 |
| MS.gene05010 | MS.gene69400 | 0.830286 | 3.20E-55 | -1.69E-46 |
| MS.gene050170 | MS.gene69400 | 0.824371 | 8.37E-54 | -1.69E-46 |
| MS.gene050216 | MS.gene69400 | 0.805068 | 1.60E-49 | -1.69E-46 |
| MS.gene050323 | MS.gene69400 | 0.819013 | 1.45E-52 | -1.69E-46 |
| MS.gene050496 | MS.gene69400 | 0.848942 | 4.44E-60 | -1.69E-46 |
| MS.gene050502 | MS.gene69400 | 0.800964 | 1.13E-48 | -1.69E-46 |
| MS.gene050868 | MS.gene69400 | 0.828092 | 1.09E-54 | -1.69E-46 |
| MS.gene050871 | MS.gene69400 | 0.830236 | 3.29E-55 | -1.69E-46 |
| MS.gene051509 | MS.gene69400 | 0.803585 | 3.25E-49 | -1.69E-46 |
| MS.gene052504 | MS.gene69400 | 0.8166 | 5.09E-52 | -1.69E-46 |
| MS.gene053505 | MS.gene69400 | 0.803308 | 3.71E-49 | -1.69E-46 |
| MS.gene054595 | MS.gene69400 | 0.816972 | 4.20E-52 | -1.69E-46 |
| MS.gene056504 | MS.gene69400 | 0.824906 | 6.26E-54 | -1.69E-46 |
| MS.gene056878 | MS.gene69400 | 0.814199 | 1.74E-51 | -1.69E-46 |
| MS.gene057086 | MS.gene69400 | 0.824802 | 6.63E-54 | -1.69E-46 |
| MS.gene059367 | MS.gene69400 | 0.805973 | 1.03E-49 | -1.69E-46 |
| MS.gene059837 | MS.gene69400 | 0.808343 | 3.24E-50 | -1.69E-46 |
| MS.gene059865 | MS.gene69400 | 0.812808 | 3.52E-51 | -1.69E-46 |
| MS.gene06000 | MS.gene69400 | 0.847311 | 1.25E-59 | -1.69E-46 |
| MS.gene060595 | MS.gene69400 | -0.809314 | 2.01E-50 | -1.69E-46 |
| MS.gene060778 | MS.gene69400 | 0.841532 | 4.49E-58 | -1.69E-46 |
| MS.gene06077 | MS.gene69400 | 0.801209 | 1.00E-48 | -1.69E-46 |
| MS.gene060982 | MS.gene69400 | 0.813311 | 2.73E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69400.t1 | MTR_3g113890 | 95.146 | 103 | 5 | 0 | 1 | 103 | 1 | 103 | 1.37e-68 | 200 |
| MS.gene69400.t1 | MTR_1g025220 | 66.990 | 103 | 32 | 1 | 1 | 103 | 1 | 101 | 4.09e-46 | 144 |
| MS.gene69400.t1 | MTR_1g025250 | 66.990 | 103 | 31 | 1 | 1 | 103 | 1 | 100 | 5.80e-38 | 123 |
| MS.gene69400.t1 | MTR_1g096370 | 52.222 | 90 | 42 | 1 | 14 | 103 | 7 | 95 | 5.09e-29 | 100 |
| MS.gene69400.t1 | MTR_4g106860 | 52.381 | 84 | 37 | 2 | 21 | 103 | 34 | 115 | 3.37e-24 | 89.0 |
| MS.gene69400.t1 | MTR_8g075920 | 52.326 | 86 | 40 | 1 | 18 | 103 | 19 | 103 | 4.72e-23 | 85.5 |
| MS.gene69400.t1 | MTR_3g113850 | 88.889 | 45 | 5 | 0 | 44 | 88 | 406 | 450 | 6.32e-21 | 85.9 |
| MS.gene69400.t1 | MTR_8g062800 | 38.889 | 108 | 54 | 2 | 8 | 103 | 5 | 112 | 7.15e-20 | 77.8 |
| MS.gene69400.t1 | MTR_6g007897 | 57.377 | 61 | 25 | 1 | 44 | 103 | 188 | 248 | 1.19e-15 | 70.1 |
| MS.gene69400.t1 | MTR_5g036880 | 36.458 | 96 | 57 | 2 | 8 | 103 | 1 | 92 | 2.44e-15 | 65.9 |
| MS.gene69400.t1 | MTR_5g036720 | 37.113 | 97 | 53 | 2 | 7 | 103 | 4 | 92 | 2.52e-15 | 65.9 |
| MS.gene69400.t1 | MTR_3g102660 | 49.153 | 59 | 30 | 0 | 45 | 103 | 27 | 85 | 8.80e-15 | 64.3 |
| MS.gene69400.t1 | MTR_7g090590 | 33.884 | 121 | 61 | 3 | 1 | 103 | 1 | 120 | 2.54e-13 | 61.2 |
| MS.gene69400.t1 | MTR_1g106915 | 35.714 | 70 | 45 | 0 | 34 | 103 | 21 | 90 | 9.61e-13 | 58.9 |
| MS.gene69400.t1 | MTR_7g010580 | 31.731 | 104 | 68 | 1 | 3 | 103 | 6 | 109 | 1.22e-12 | 59.3 |
| MS.gene69400.t1 | MTR_5g036860 | 33.333 | 96 | 60 | 2 | 8 | 103 | 1 | 92 | 1.38e-12 | 58.5 |
| MS.gene69400.t1 | MTR_5g095990 | 40.260 | 77 | 44 | 2 | 27 | 103 | 75 | 149 | 4.43e-12 | 58.9 |
| MS.gene69400.t1 | MTR_6g012360 | 29.907 | 107 | 71 | 1 | 1 | 103 | 1 | 107 | 8.52e-12 | 57.0 |
| MS.gene69400.t1 | MTR_5g036640 | 40.580 | 69 | 41 | 0 | 35 | 103 | 15 | 83 | 8.54e-12 | 56.6 |
| MS.gene69400.t1 | MTR_3g037750 | 34.694 | 98 | 63 | 1 | 6 | 103 | 7 | 103 | 9.55e-12 | 57.0 |
| MS.gene69400.t1 | MTR_3g007900 | 42.029 | 69 | 39 | 1 | 35 | 103 | 87 | 154 | 2.29e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69400.t1 | AT1G75750 | 68.056 | 72 | 23 | 0 | 32 | 103 | 27 | 98 | 6.02e-32 | 108 |
| MS.gene69400.t1 | AT1G75750 | 68.056 | 72 | 23 | 0 | 32 | 103 | 26 | 97 | 6.18e-32 | 108 |
| MS.gene69400.t1 | AT2G18420 | 68.919 | 74 | 22 | 1 | 31 | 103 | 21 | 94 | 3.11e-30 | 103 |
| MS.gene69400.t1 | AT2G18420 | 75.806 | 62 | 15 | 0 | 42 | 103 | 9 | 70 | 4.26e-29 | 100 |
| MS.gene69400.t1 | AT4G09600 | 59.494 | 79 | 29 | 1 | 28 | 103 | 21 | 99 | 2.12e-26 | 94.0 |
| MS.gene69400.t1 | AT1G22690 | 41.593 | 113 | 56 | 3 | 1 | 103 | 7 | 119 | 2.70e-23 | 86.7 |
| MS.gene69400.t1 | AT1G22690 | 41.593 | 113 | 56 | 3 | 1 | 103 | 7 | 119 | 2.70e-23 | 86.7 |
| MS.gene69400.t1 | AT1G22690 | 41.593 | 113 | 56 | 3 | 1 | 103 | 7 | 119 | 2.70e-23 | 86.7 |
| MS.gene69400.t1 | AT4G09610 | 62.025 | 79 | 27 | 1 | 28 | 103 | 21 | 99 | 1.58e-19 | 76.6 |
| MS.gene69400.t1 | AT5G14920 | 56.923 | 65 | 27 | 1 | 40 | 103 | 211 | 275 | 4.92e-17 | 73.9 |
| MS.gene69400.t1 | AT1G74670 | 36.735 | 98 | 61 | 1 | 7 | 103 | 4 | 101 | 2.72e-16 | 68.6 |
| MS.gene69400.t1 | AT5G59845 | 36.000 | 100 | 46 | 2 | 5 | 103 | 7 | 89 | 4.71e-14 | 62.4 |
| MS.gene69400.t1 | AT1G10588 | 34.043 | 94 | 52 | 2 | 11 | 103 | 5 | 89 | 1.26e-12 | 58.9 |
| MS.gene69400.t1 | AT1G10588 | 45.000 | 60 | 32 | 1 | 45 | 103 | 31 | 90 | 1.81e-12 | 58.5 |
| MS.gene69400.t1 | AT2G39540 | 32.258 | 93 | 54 | 1 | 11 | 103 | 4 | 87 | 2.54e-12 | 57.8 |
| MS.gene69400.t1 | AT5G15230 | 29.907 | 107 | 58 | 2 | 6 | 103 | 8 | 106 | 2.00e-11 | 56.2 |
Find 31 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATAGCAGGGACACATTTCT+TGG | 0.264407 | 3.3:+93229430 | None:intergenic |
| TCTCCATCTTGTTCATGCTT+TGG | 0.293177 | 3.3:-93230126 | MS.gene69400:CDS |
| GGTGGTTTGGTTGGCATAGC+AGG | 0.303830 | 3.3:+93229416 | None:intergenic |
| TGCGTGCCACCGGGAACTTC+TGG | 0.308001 | 3.3:-93229456 | MS.gene69400:CDS |
| GGCACTTGCGTCTGCCTCCA+TGG | 0.330551 | 3.3:+93229394 | None:intergenic |
| GCTTCTAGTTACATCTCTTC+TGG | 0.340920 | 3.3:-93230162 | MS.gene69400:CDS |
| CACGGCCGCGCCTCTGCCAC+AGG | 0.395156 | 3.3:-93229509 | MS.gene69400:CDS |
| GTCTGCCTCCATGGGTGGTT+TGG | 0.427775 | 3.3:+93229403 | None:intergenic |
| GCCTCCATGGGTGGTTTGGT+TGG | 0.435015 | 3.3:+93229407 | None:intergenic |
| TCGGTGCAACTGCGTGCCAC+CGG | 0.451569 | 3.3:-93229466 | MS.gene69400:CDS |
| TTCCACATGCCCTGTGGCAG+AGG | 0.454808 | 3.3:+93229499 | None:intergenic |
| GGCGCGGCCGTGATGATAAA+CGG | 0.465640 | 3.3:+93229520 | None:intergenic |
| TCTTGGTTACCAGAAGTTCC+CGG | 0.467040 | 3.3:+93229447 | None:intergenic |
| CGCCTCTGCCACAGGGCATG+TGG | 0.480960 | 3.3:-93229501 | MS.gene69400:CDS |
| GCACTTGCGTCTGCCTCCAT+GGG | 0.498020 | 3.3:+93229395 | None:intergenic |
| CTTGCGTCTGCCTCCATGGG+TGG | 0.501890 | 3.3:+93229398 | None:intergenic |
| GTGGTTTGGTTGGCATAGCA+GGG | 0.546195 | 3.3:+93229417 | None:intergenic |
| CGGTGCAACTGCGTGCCACC+GGG | 0.551251 | 3.3:-93229465 | MS.gene69400:CDS |
| TGTTCATGCTTTGGACCAGC+CGG | 0.555470 | 3.3:-93230117 | MS.gene69400:intron |
| GCAATGGAGCATGTGCTGCT+AGG | 0.562822 | 3.3:-93229545 | MS.gene69400:CDS |
| ATATAAATTTCAGATTGCAA+TGG | 0.566444 | 3.3:-93229561 | MS.gene69400:intron |
| GGTCCAAAGCATGAACAAGA+TGG | 0.576917 | 3.3:+93230123 | None:intergenic |
| TGAGTTTCTGCGTGTACATG+CGG | 0.582718 | 3.3:+93229981 | None:intergenic |
| CATGCCCTGTGGCAGAGGCG+CGG | 0.598879 | 3.3:+93229504 | None:intergenic |
| CATGTGGAACTTGCTGCAGT+CGG | 0.613537 | 3.3:-93229485 | MS.gene69400:CDS |
| ACGGCCGCGCCTCTGCCACA+GGG | 0.616011 | 3.3:-93229508 | MS.gene69400:CDS |
| CTAGGTGCCGTTTATCATCA+CGG | 0.637583 | 3.3:-93229527 | MS.gene69400:CDS |
| TATGCCAACCAAACCACCCA+TGG | 0.640986 | 3.3:-93229411 | MS.gene69400:CDS |
| GCCAACCAAACCACCCATGG+AGG | 0.699460 | 3.3:-93229408 | MS.gene69400:CDS |
| AGCAAGTTCCACATGCCCTG+TGG | 0.700479 | 3.3:+93229493 | None:intergenic |
| TGGTTACCAGAAGTTCCCGG+TGG | 0.747138 | 3.3:+93229450 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAAGATTAGTAAAAAAA+AGG | - | chr3.3:93229844-93229863 | MS.gene69400:intron | 10.0% |
| !! | AAAATATATGAACTAAACTA+AGG | - | chr3.3:93229730-93229749 | MS.gene69400:intron | 15.0% |
| !!! | TTTTAATTATTTGCTTAAAG+TGG | - | chr3.3:93229665-93229684 | MS.gene69400:intron | 15.0% |
| !! | ATATAAATTTCAGATTGCAA+TGG | - | chr3.3:93230004-93230023 | MS.gene69400:intron | 20.0% |
| !!! | TATTTTATAAAGAAAACGCT+TGG | + | chr3.3:93229523-93229542 | None:intergenic | 20.0% |
| !!! | TTAATTACATGTGTTTTTGT+AGG | - | chr3.3:93229559-93229578 | MS.gene69400:intron | 20.0% |
| ! | TAACGAATATGTATGCTAAT+GGG | - | chr3.3:93229821-93229840 | MS.gene69400:intron | 25.0% |
| ! | TTAACGAATATGTATGCTAA+TGG | - | chr3.3:93229820-93229839 | MS.gene69400:intron | 25.0% |
| ! | CTACTTCACACACATATTTT+TGG | + | chr3.3:93229972-93229991 | None:intergenic | 30.0% |
| !! | CAACAATAGCTTTGAATTAC+CGG | + | chr3.3:93229470-93229489 | None:intergenic | 30.0% |
| TCTCCATCTTGTTCATGCTT+TGG | - | chr3.3:93229439-93229458 | MS.gene69400:CDS | 40.0% | |
| TTTCATCAACTGCTTCTGTC+TGG | - | chr3.3:93229642-93229661 | MS.gene69400:intron | 40.0% | |
| ! | GCTTCTAGTTACATCTCTTC+TGG | - | chr3.3:93229403-93229422 | MS.gene69400:CDS | 40.0% |
| ! | GTTTCTTTTCAGCAGCAGAT+AGG | - | chr3.3:93229608-93229627 | MS.gene69400:intron | 40.0% |
| !! | AATAGCTTTGAATTACCGGC+TGG | + | chr3.3:93229466-93229485 | None:intergenic | 40.0% |
| CATAGCAGGGACACATTTCT+TGG | + | chr3.3:93230138-93230157 | None:intergenic | 45.0% | |
| GGTCCAAAGCATGAACAAGA+TGG | + | chr3.3:93229445-93229464 | None:intergenic | 45.0% | |
| TCTTGGTTACCAGAAGTTCC+CGG | + | chr3.3:93230121-93230140 | None:intergenic | 45.0% | |
| TGAGTTTCTGCGTGTACATG+CGG | + | chr3.3:93229587-93229606 | None:intergenic | 45.0% | |
| !! | CTAGGTGCCGTTTATCATCA+CGG | - | chr3.3:93230038-93230057 | MS.gene69400:intron | 45.0% |
| !! | GTAATTAAATTAAATTAAAT+TGG | + | chr3.3:93229547-93229566 | None:intergenic | 5.0% |
| TATGCCAACCAAACCACCCA+TGG | - | chr3.3:93230154-93230173 | MS.gene69400:CDS | 50.0% | |
| ! | TGTTCATGCTTTGGACCAGC+CGG | - | chr3.3:93229448-93229467 | MS.gene69400:CDS | 50.0% |
| !! | CATGTGGAACTTGCTGCAGT+CGG | - | chr3.3:93230080-93230099 | MS.gene69400:intron | 50.0% |
| !! | GTGGTTTGGTTGGCATAGCA+GGG | + | chr3.3:93230151-93230170 | None:intergenic | 50.0% |
| AGCAAGTTCCACATGCCCTG+TGG | + | chr3.3:93230075-93230094 | None:intergenic | 55.0% | |
| TGGTTACCAGAAGTTCCCGG+TGG | + | chr3.3:93230118-93230137 | None:intergenic | 55.0% | |
| ! | GCAATGGAGCATGTGCTGCT+AGG | - | chr3.3:93230020-93230039 | MS.gene69400:intron | 55.0% |
| !! | GGTGGTTTGGTTGGCATAGC+AGG | + | chr3.3:93230152-93230171 | None:intergenic | 55.0% |
| GCACTTGCGTCTGCCTCCAT+GGG | + | chr3.3:93230173-93230192 | None:intergenic | 60.0% | |
| GCCAACCAAACCACCCATGG+AGG | - | chr3.3:93230157-93230176 | MS.gene69400:CDS | 60.0% | |
| GGCGCGGCCGTGATGATAAA+CGG | + | chr3.3:93230048-93230067 | None:intergenic | 60.0% | |
| GTCTGCCTCCATGGGTGGTT+TGG | + | chr3.3:93230165-93230184 | None:intergenic | 60.0% | |
| TTCCACATGCCCTGTGGCAG+AGG | + | chr3.3:93230069-93230088 | None:intergenic | 60.0% | |
| !! | GCCTCCATGGGTGGTTTGGT+TGG | + | chr3.3:93230161-93230180 | None:intergenic | 60.0% |
| CTTGCGTCTGCCTCCATGGG+TGG | + | chr3.3:93230170-93230189 | None:intergenic | 65.0% | |
| GGCACTTGCGTCTGCCTCCA+TGG | + | chr3.3:93230174-93230193 | None:intergenic | 65.0% | |
| TCGGTGCAACTGCGTGCCAC+CGG | - | chr3.3:93230099-93230118 | MS.gene69400:intron | 65.0% | |
| TGCGTGCCACCGGGAACTTC+TGG | - | chr3.3:93230109-93230128 | MS.gene69400:intron | 65.0% | |
| CATGCCCTGTGGCAGAGGCG+CGG | + | chr3.3:93230064-93230083 | None:intergenic | 70.0% | |
| CGCCTCTGCCACAGGGCATG+TGG | - | chr3.3:93230064-93230083 | MS.gene69400:intron | 70.0% | |
| CGGTGCAACTGCGTGCCACC+GGG | - | chr3.3:93230100-93230119 | MS.gene69400:intron | 70.0% | |
| ACGGCCGCGCCTCTGCCACA+GGG | - | chr3.3:93230057-93230076 | MS.gene69400:intron | 75.0% | |
| ! | CACGGCCGCGCCTCTGCCAC+AGG | - | chr3.3:93230056-93230075 | MS.gene69400:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 93229389 | 93230198 | 93229389 | ID=MS.gene69400 |
| chr3.3 | mRNA | 93229389 | 93230198 | 93229389 | ID=MS.gene69400.t1;Parent=MS.gene69400 |
| chr3.3 | exon | 93230118 | 93230198 | 93230118 | ID=MS.gene69400.t1.exon1;Parent=MS.gene69400.t1 |
| chr3.3 | CDS | 93230118 | 93230198 | 93230118 | ID=cds.MS.gene69400.t1;Parent=MS.gene69400.t1 |
| chr3.3 | exon | 93229958 | 93230006 | 93229958 | ID=MS.gene69400.t1.exon2;Parent=MS.gene69400.t1 |
| chr3.3 | CDS | 93229958 | 93230006 | 93229958 | ID=cds.MS.gene69400.t1;Parent=MS.gene69400.t1 |
| chr3.3 | exon | 93229389 | 93229570 | 93229389 | ID=MS.gene69400.t1.exon3;Parent=MS.gene69400.t1 |
| chr3.3 | CDS | 93229389 | 93229570 | 93229389 | ID=cds.MS.gene69400.t1;Parent=MS.gene69400.t1 |
| Gene Sequence |
| Protein sequence |
