Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71178.t1 | KEH38713.1 | 61 | 187 | 63 | 2 | 5 | 191 | 49 | 225 | 1.60E-40 | 176 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71178.t1 | A0A072VB89 | 61.0 | 187 | 63 | 2 | 5 | 191 | 49 | 225 | 1.2e-40 | 176.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene71178 | 0.802432 | 5.63E-49 | -1.69E-46 |
| MS.gene050227 | MS.gene71178 | 0.806298 | 8.80E-50 | -1.69E-46 |
| MS.gene050228 | MS.gene71178 | 0.807255 | 5.53E-50 | -1.69E-46 |
| MS.gene050303 | MS.gene71178 | 0.802046 | 6.76E-49 | -1.69E-46 |
| MS.gene050330 | MS.gene71178 | -0.806097 | 9.70E-50 | -1.69E-46 |
| MS.gene050442 | MS.gene71178 | 0.805516 | 1.29E-49 | -1.69E-46 |
| MS.gene050719 | MS.gene71178 | 0.816001 | 6.92E-52 | -1.69E-46 |
| MS.gene050721 | MS.gene71178 | 0.814376 | 1.59E-51 | -1.69E-46 |
| MS.gene050724 | MS.gene71178 | 0.805333 | 1.40E-49 | -1.69E-46 |
| MS.gene050824 | MS.gene71178 | 0.802805 | 4.71E-49 | -1.69E-46 |
| MS.gene050844 | MS.gene71178 | 0.80408 | 2.56E-49 | -1.69E-46 |
| MS.gene051265 | MS.gene71178 | 0.806664 | 7.37E-50 | -1.69E-46 |
| MS.gene052012 | MS.gene71178 | 0.802448 | 5.58E-49 | -1.69E-46 |
| MS.gene052190 | MS.gene71178 | 0.827036 | 1.95E-54 | -1.69E-46 |
| MS.gene052358 | MS.gene71178 | -0.809317 | 2.01E-50 | -1.69E-46 |
| MS.gene052519 | MS.gene71178 | 0.809042 | 2.30E-50 | -1.69E-46 |
| MS.gene052863 | MS.gene71178 | 0.817474 | 3.24E-52 | -1.69E-46 |
| MS.gene052896 | MS.gene71178 | 0.814574 | 1.44E-51 | -1.69E-46 |
| MS.gene053006 | MS.gene71178 | 0.803037 | 4.22E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene71178 | 0.811073 | 8.40E-51 | -1.69E-46 |
| MS.gene055358 | MS.gene71178 | 0.805458 | 1.32E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene71178 | 0.842509 | 2.48E-58 | -1.69E-46 |
| MS.gene056506 | MS.gene71178 | 0.824341 | 8.51E-54 | -1.69E-46 |
| MS.gene057026 | MS.gene71178 | 0.825371 | 4.86E-54 | -1.69E-46 |
| MS.gene058037 | MS.gene71178 | 0.805521 | 1.28E-49 | -1.69E-46 |
| MS.gene059789 | MS.gene71178 | 0.965182 | 2.83E-124 | -1.69E-46 |
| MS.gene059842 | MS.gene71178 | 0.800467 | 1.42E-48 | -1.69E-46 |
| MS.gene060322 | MS.gene71178 | 0.851068 | 1.13E-60 | -1.69E-46 |
| MS.gene060572 | MS.gene71178 | 0.827251 | 1.73E-54 | -1.69E-46 |
| MS.gene060683 | MS.gene71178 | 0.819127 | 1.37E-52 | -1.69E-46 |
| MS.gene060685 | MS.gene71178 | 0.818182 | 2.24E-52 | -1.69E-46 |
| MS.gene060803 | MS.gene71178 | 0.807166 | 5.77E-50 | -1.69E-46 |
| MS.gene061040 | MS.gene71178 | -0.837611 | 4.69E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71178.t1 | MTR_2g079000 | 79.021 | 143 | 28 | 1 | 49 | 191 | 85 | 225 | 2.24e-68 | 208 |
| MS.gene71178.t1 | MTR_5g012820 | 61.929 | 197 | 48 | 4 | 10 | 179 | 109 | 305 | 2.42e-65 | 204 |
| MS.gene71178.t1 | MTR_5g012820 | 61.929 | 197 | 48 | 4 | 10 | 179 | 119 | 315 | 4.75e-65 | 204 |
| MS.gene71178.t1 | MTR_2g079040 | 87.500 | 40 | 5 | 0 | 133 | 172 | 1 | 40 | 7.42e-16 | 71.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71178.t1 | AT5G05210 | 46.078 | 204 | 78 | 6 | 1 | 179 | 144 | 340 | 1.25e-41 | 144 |
| MS.gene71178.t1 | AT5G05210 | 46.078 | 204 | 78 | 6 | 1 | 179 | 136 | 332 | 1.25e-41 | 144 |
| MS.gene71178.t1 | AT2G27750 | 37.356 | 174 | 75 | 6 | 12 | 179 | 3 | 148 | 5.84e-21 | 85.9 |
Find 44 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGGTGAAGCTATTGCTAAAA+AGG | 0.287342 | 2.4:+19329141 | MS.gene71178:CDS |
| ATACTTCTCTTTATCAGTTT+AGG | 0.301257 | 2.4:-19329217 | None:intergenic |
| TGAAAGAAAGATAGAGTTTC+CGG | 0.308185 | 2.4:+19328955 | MS.gene71178:CDS |
| AGGTCCTAACTGTGCAAATC+TGG | 0.332531 | 2.4:+19328913 | MS.gene71178:CDS |
| GCAGTTAAGCCTTTCATGTC+GGG | 0.372922 | 2.4:+19328812 | MS.gene71178:CDS |
| AGCAGAAAATCGCAGTGAAA+TGG | 0.374864 | 2.4:+19329260 | MS.gene71178:CDS |
| TGAAAGAGCTAAGAAGTTAG+AGG | 0.400614 | 2.4:+19329093 | MS.gene71178:CDS |
| GTATACAGAAATGGAAGAAT+AGG | 0.408150 | 2.4:+19329236 | MS.gene71178:CDS |
| GCAATAGCTTCACCCTTCTC+AGG | 0.419537 | 2.4:-19329133 | None:intergenic |
| AGCAGCAATGAATAGAGCTT+CGG | 0.425070 | 2.4:+19329174 | MS.gene71178:CDS |
| GCAGCAATGAATAGAGCTTC+GGG | 0.426903 | 2.4:+19329175 | MS.gene71178:CDS |
| TCTTTAAAAGAGCTTGTGTT+TGG | 0.437000 | 2.4:+19329007 | MS.gene71178:CDS |
| TCCTTTACCACCTTCTCCTC+CGG | 0.440386 | 2.4:-19328974 | None:intergenic |
| ATCTGGAAAATGCTAATAAG+AGG | 0.444036 | 2.4:+19328930 | MS.gene71178:CDS |
| TAAAGAGAAGTATACAGAAA+TGG | 0.461758 | 2.4:+19329227 | MS.gene71178:CDS |
| GCAGAAAATCGCAGTGAAAT+GGG | 0.472714 | 2.4:+19329261 | MS.gene71178:CDS |
| TCTTTCAAGTTCCTTGTGCT+TGG | 0.476271 | 2.4:-19329077 | None:intergenic |
| GGCAATGTTAAACTTCAGAA+TGG | 0.487362 | 2.4:+19329028 | MS.gene71178:CDS |
| GATAGAGTTTCCGGAGGAGA+AGG | 0.496889 | 2.4:+19328964 | MS.gene71178:CDS |
| GAATAGAGCTTCGGGAATCA+AGG | 0.497557 | 2.4:+19329183 | MS.gene71178:CDS |
| TCCGGAGGAGAAGGTGGTAA+AGG | 0.513098 | 2.4:+19328973 | MS.gene71178:CDS |
| TCAAACAAAGGATCAGTTGA+AGG | 0.525525 | 2.4:+19329301 | MS.gene71178:CDS |
| AAACTTGAAGAGTTTCAGTC+AGG | 0.526291 | 2.4:+19328893 | MS.gene71178:CDS |
| TAAGCCTTTCATGTCGGGGT+TGG | 0.538275 | 2.4:+19328817 | MS.gene71178:CDS |
| TGCAGTTAAGCCTTTCATGT+CGG | 0.538494 | 2.4:+19328811 | MS.gene71178:CDS |
| CCTTTCGCGTAGTTCTTCAA+AGG | 0.546785 | 2.4:-19328861 | None:intergenic |
| CGCAGTGAAATGGGAAGAGA+GGG | 0.547718 | 2.4:+19329270 | MS.gene71178:CDS |
| GCTTCCAACCCCGACATGAA+AGG | 0.556004 | 2.4:-19328821 | None:intergenic |
| CGGGGTTGGAAGCAGATGAT+CGG | 0.561569 | 2.4:+19328831 | MS.gene71178:CDS |
| CTTCAGAATGGTGAAATGCA+AGG | 0.562609 | 2.4:+19329040 | MS.gene71178:CDS |
| TTCAGAATGGTGAAATGCAA+GGG | 0.568164 | 2.4:+19329041 | MS.gene71178:CDS |
| AGAGTTTCCGGAGGAGAAGG+TGG | 0.570130 | 2.4:+19328967 | MS.gene71178:CDS |
| AATGACAGCGATGGAGAGGA+AGG | 0.580649 | 2.4:+19328788 | MS.gene71178:CDS |
| AATGAATGACAGCGATGGAG+AGG | 0.585376 | 2.4:+19328784 | None:intergenic |
| GAAGAAGAATGATCCTGAGA+AGG | 0.589096 | 2.4:+19329120 | MS.gene71178:CDS |
| AAGAAGAATGATCCTGAGAA+GGG | 0.591026 | 2.4:+19329121 | MS.gene71178:CDS |
| CAGTTAAGCCTTTCATGTCG+GGG | 0.594657 | 2.4:+19328813 | MS.gene71178:CDS |
| TCTGGAAAATGCTAATAAGA+GGG | 0.594774 | 2.4:+19328931 | MS.gene71178:CDS |
| TCGCAGTGAAATGGGAAGAG+AGG | 0.597560 | 2.4:+19329269 | MS.gene71178:CDS |
| CCTTTGAAGAACTACGCGAA+AGG | 0.632563 | 2.4:+19328861 | MS.gene71178:CDS |
| AGGGTTCAAAGTTCAAACAA+AGG | 0.644899 | 2.4:+19329289 | MS.gene71178:CDS |
| GAGAAAAGTTTCCAAGCACA+AGG | 0.645702 | 2.4:+19329066 | MS.gene71178:CDS |
| GGAGGAGAAGGTGGTAAAGG+AGG | 0.646856 | 2.4:+19328976 | MS.gene71178:CDS |
| AAGAAAGATAGAGTTTCCGG+AGG | 0.655190 | 2.4:+19328958 | MS.gene71178:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATACTTCTCTTTATCAGTTT+AGG | - | chr2.4:19329220-19329239 | None:intergenic | 25.0% |
| !! | TAAAGAGAAGTATACAGAAA+TGG | + | chr2.4:19329227-19329246 | MS.gene71178:CDS | 25.0% |
| ATCTGGAAAATGCTAATAAG+AGG | + | chr2.4:19328930-19328949 | MS.gene71178:CDS | 30.0% | |
| GTATACAGAAATGGAAGAAT+AGG | + | chr2.4:19329236-19329255 | MS.gene71178:CDS | 30.0% | |
| TCTGGAAAATGCTAATAAGA+GGG | + | chr2.4:19328931-19328950 | MS.gene71178:CDS | 30.0% | |
| TGAAAGAAAGATAGAGTTTC+CGG | + | chr2.4:19328955-19328974 | MS.gene71178:CDS | 30.0% | |
| ! | TCTTTAAAAGAGCTTGTGTT+TGG | + | chr2.4:19329007-19329026 | MS.gene71178:CDS | 30.0% |
| AAACTTGAAGAGTTTCAGTC+AGG | + | chr2.4:19328893-19328912 | MS.gene71178:CDS | 35.0% | |
| AAGAAGAATGATCCTGAGAA+GGG | + | chr2.4:19329121-19329140 | MS.gene71178:CDS | 35.0% | |
| AGGCAGAGAAACAACAAAAA+AGG | + | chr2.4:19329321-19329340 | MS.gene71178:CDS | 35.0% | |
| AGGGTTCAAAGTTCAAACAA+AGG | + | chr2.4:19329289-19329308 | MS.gene71178:CDS | 35.0% | |
| CTATTGCTAAAAAGGAAGCA+TGG | + | chr2.4:19329149-19329168 | MS.gene71178:CDS | 35.0% | |
| GGCAATGTTAAACTTCAGAA+TGG | + | chr2.4:19329028-19329047 | MS.gene71178:CDS | 35.0% | |
| TCAAACAAAGGATCAGTTGA+AGG | + | chr2.4:19329301-19329320 | MS.gene71178:CDS | 35.0% | |
| TGAAAGAGCTAAGAAGTTAG+AGG | + | chr2.4:19329093-19329112 | MS.gene71178:CDS | 35.0% | |
| TTCAGAATGGTGAAATGCAA+GGG | + | chr2.4:19329041-19329060 | MS.gene71178:CDS | 35.0% | |
| ! | TTTTCCAGATTTGCACAGTT+AGG | - | chr2.4:19328920-19328939 | None:intergenic | 35.0% |
| !! | AAGCTCTTTTAAAGACTCTG+TGG | - | chr2.4:19329002-19329021 | None:intergenic | 35.0% |
| AAGAAAGATAGAGTTTCCGG+AGG | + | chr2.4:19328958-19328977 | MS.gene71178:CDS | 40.0% | |
| AGCAGAAAATCGCAGTGAAA+TGG | + | chr2.4:19329260-19329279 | MS.gene71178:CDS | 40.0% | |
| AGCAGCAATGAATAGAGCTT+CGG | + | chr2.4:19329174-19329193 | MS.gene71178:CDS | 40.0% | |
| CTTCAGAATGGTGAAATGCA+AGG | + | chr2.4:19329040-19329059 | MS.gene71178:CDS | 40.0% | |
| GAAGAAGAATGATCCTGAGA+AGG | + | chr2.4:19329120-19329139 | MS.gene71178:CDS | 40.0% | |
| GAGAAAAGTTTCCAAGCACA+AGG | + | chr2.4:19329066-19329085 | MS.gene71178:CDS | 40.0% | |
| GCAGAAAATCGCAGTGAAAT+GGG | + | chr2.4:19329261-19329280 | MS.gene71178:CDS | 40.0% | |
| GGGTGAAGCTATTGCTAAAA+AGG | + | chr2.4:19329141-19329160 | MS.gene71178:CDS | 40.0% | |
| TGCAGTTAAGCCTTTCATGT+CGG | + | chr2.4:19328811-19328830 | MS.gene71178:CDS | 40.0% | |
| !! | TCTTTCAAGTTCCTTGTGCT+TGG | - | chr2.4:19329080-19329099 | None:intergenic | 40.0% |
| AGGTCCTAACTGTGCAAATC+TGG | + | chr2.4:19328913-19328932 | MS.gene71178:CDS | 45.0% | |
| CAGTTAAGCCTTTCATGTCG+GGG | + | chr2.4:19328813-19328832 | MS.gene71178:CDS | 45.0% | |
| CCTTTCGCGTAGTTCTTCAA+AGG | - | chr2.4:19328864-19328883 | None:intergenic | 45.0% | |
| GAATAGAGCTTCGGGAATCA+AGG | + | chr2.4:19329183-19329202 | MS.gene71178:CDS | 45.0% | |
| GCAGCAATGAATAGAGCTTC+GGG | + | chr2.4:19329175-19329194 | MS.gene71178:CDS | 45.0% | |
| GCAGTTAAGCCTTTCATGTC+GGG | + | chr2.4:19328812-19328831 | MS.gene71178:CDS | 45.0% | |
| ! | CCTTTGAAGAACTACGCGAA+AGG | + | chr2.4:19328861-19328880 | MS.gene71178:CDS | 45.0% |
| AATGACAGCGATGGAGAGGA+AGG | + | chr2.4:19328788-19328807 | MS.gene71178:CDS | 50.0% | |
| CGCAGTGAAATGGGAAGAGA+GGG | + | chr2.4:19329270-19329289 | MS.gene71178:CDS | 50.0% | |
| GATAGAGTTTCCGGAGGAGA+AGG | + | chr2.4:19328964-19328983 | MS.gene71178:CDS | 50.0% | |
| GCAATAGCTTCACCCTTCTC+AGG | - | chr2.4:19329136-19329155 | None:intergenic | 50.0% | |
| TAAGCCTTTCATGTCGGGGT+TGG | + | chr2.4:19328817-19328836 | MS.gene71178:CDS | 50.0% | |
| TCCTTTACCACCTTCTCCTC+CGG | - | chr2.4:19328977-19328996 | None:intergenic | 50.0% | |
| TCGCAGTGAAATGGGAAGAG+AGG | + | chr2.4:19329269-19329288 | MS.gene71178:CDS | 50.0% | |
| AGAGTTTCCGGAGGAGAAGG+TGG | + | chr2.4:19328967-19328986 | MS.gene71178:CDS | 55.0% | |
| CGGGGTTGGAAGCAGATGAT+CGG | + | chr2.4:19328831-19328850 | MS.gene71178:CDS | 55.0% | |
| GCTTCCAACCCCGACATGAA+AGG | - | chr2.4:19328824-19328843 | None:intergenic | 55.0% | |
| GGAGGAGAAGGTGGTAAAGG+AGG | + | chr2.4:19328976-19328995 | MS.gene71178:CDS | 55.0% | |
| TCCGGAGGAGAAGGTGGTAA+AGG | + | chr2.4:19328973-19328992 | MS.gene71178:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 19328785 | 19329360 | 19328785 | ID=MS.gene71178 |
| chr2.4 | mRNA | 19328785 | 19329360 | 19328785 | ID=MS.gene71178.t1;Parent=MS.gene71178 |
| chr2.4 | exon | 19328785 | 19329360 | 19328785 | ID=MS.gene71178.t1.exon1;Parent=MS.gene71178.t1 |
| chr2.4 | CDS | 19328785 | 19329360 | 19328785 | ID=cds.MS.gene71178.t1;Parent=MS.gene71178.t1 |
| Gene Sequence |
| Protein sequence |
