Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73000.t1 | RZC20088.1 | 100 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 7.30E-34 | 152.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73000.t1 | Q9ZRS8 | 98.6 | 71 | 1 | 0 | 1 | 71 | 1 | 71 | 2.6e-35 | 148.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73000.t1 | G7JLC8 | 100.0 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 5.3e-34 | 152.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049361 | MS.gene73000 | 0.805739 | 1.15E-49 | -1.69E-46 |
| MS.gene050134 | MS.gene73000 | 0.892811 | 1.12E-74 | -1.69E-46 |
| MS.gene050252 | MS.gene73000 | 0.825255 | 5.18E-54 | -1.69E-46 |
| MS.gene050791 | MS.gene73000 | 0.873943 | 9.93E-68 | -1.69E-46 |
| MS.gene050912 | MS.gene73000 | 0.840433 | 8.72E-58 | -1.69E-46 |
| MS.gene051904 | MS.gene73000 | 0.827237 | 1.75E-54 | -1.69E-46 |
| MS.gene051984 | MS.gene73000 | 0.843954 | 1.02E-58 | -1.69E-46 |
| MS.gene052664 | MS.gene73000 | 0.868498 | 6.25E-66 | -1.69E-46 |
| MS.gene053224 | MS.gene73000 | 0.881321 | 2.64E-70 | -1.69E-46 |
| MS.gene057165 | MS.gene73000 | 0.805114 | 1.56E-49 | -1.69E-46 |
| MS.gene057272 | MS.gene73000 | 0.864135 | 1.51E-64 | -1.69E-46 |
| MS.gene057641 | MS.gene73000 | 0.829913 | 3.95E-55 | -1.69E-46 |
| MS.gene057643 | MS.gene73000 | 0.886682 | 2.77E-72 | -1.69E-46 |
| MS.gene060483 | MS.gene73000 | 0.821655 | 3.60E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73000.t1 | MTR_4g099160 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 7.22e-47 | 144 |
| MS.gene73000.t1 | MTR_4g103340 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 7.22e-47 | 144 |
| MS.gene73000.t1 | MTR_8g007485 | 100.000 | 71 | 0 | 0 | 1 | 71 | 1 | 71 | 7.22e-47 | 144 |
| MS.gene73000.t1 | MTR_0199s0040 | 98.611 | 72 | 1 | 0 | 1 | 72 | 82 | 153 | 1.12e-46 | 146 |
| MS.gene73000.t1 | MTR_4g103340 | 100.000 | 72 | 0 | 0 | 1 | 72 | 1 | 72 | 1.52e-46 | 142 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73000.t1 | AT3G10950 | 91.549 | 71 | 6 | 0 | 1 | 71 | 1 | 71 | 1.76e-43 | 135 |
| MS.gene73000.t1 | AT3G60245 | 90.278 | 72 | 7 | 0 | 1 | 72 | 1 | 72 | 3.87e-43 | 134 |
Find 16 sgRNAs with CRISPR-Local
Find 56 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGAAAGGCTGTAGGAATA+TGG | 0.316747 | 8.3:+18906217 | MS.gene73000:CDS |
| GAGAAAGGCTGTAGGAATAT+GGG | 0.416937 | 8.3:+18906218 | MS.gene73000:CDS |
| GCTGGCATCGTTGGCAAATA+CGG | 0.428463 | 8.3:+18905422 | MS.gene73000:CDS |
| ATGATATTAGGTACCCGTTA+TGG | 0.441488 | 8.3:+18905994 | MS.gene73000:intron |
| ACGCAAACTAGCACCATAAC+GGG | 0.476774 | 8.3:-18906007 | None:intergenic |
| ACAAAGAAGGCTGGCATCGT+TGG | 0.496247 | 8.3:+18905413 | MS.gene73000:CDS |
| TACGCAAACTAGCACCATAA+CGG | 0.535734 | 8.3:-18906008 | None:intergenic |
| AGAAAGGCTGTAGGAATATG+GGG | 0.537082 | 8.3:+18906219 | MS.gene73000:CDS |
| GACTGCGGTAAAGTGAAAGC+AGG | 0.566191 | 8.3:+18906249 | MS.gene73000:CDS |
| ACTAAGAGAACAAAGAAGGC+TGG | 0.571248 | 8.3:+18905404 | MS.gene73000:CDS |
| TGTAGGAATATGGGGATGCA+AGG | 0.596749 | 8.3:+18906227 | MS.gene73000:CDS |
| GCAGACTAAGAGAACAAAGA+AGG | 0.598781 | 8.3:+18905400 | MS.gene73000:intron |
| GCAGTATGCTGTGAAGAGAA+AGG | 0.632531 | 8.3:+18906203 | MS.gene73000:intron |
| TGCGGTAAAGTGAAAGCAGG+CGG | 0.647382 | 8.3:+18906252 | MS.gene73000:CDS |
| GCTGTGAAGAGAAAGGCTGT+AGG | 0.663437 | 8.3:+18906210 | MS.gene73000:CDS |
| ATATGGGGATGCAAGGACTG+CGG | 0.722475 | 8.3:+18906234 | MS.gene73000:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTTTATTGTATGATATT+AGG | + | chr8.3:18905982-18906001 | MS.gene73000:intron | 10.0% |
| !! | AAGTACAGATTCAATATAAA+AGG | - | chr8.3:18905367-18905386 | None:intergenic | 20.0% |
| !! | ATCTAAATAATTATGCACAT+AGG | + | chr8.3:18905564-18905583 | MS.gene73000:intron | 20.0% |
| !! | TTATTTATTTGCATAAGCTA+AGG | - | chr8.3:18905826-18905807 | None:intergenic | 20.0% |
| !!! | AAATTTTTCTGTGAATTTTG+TGG | + | chr8.3:18906066-18906085 | MS.gene73000:CDS | 20.0% |
| !!! | AATAAATAACCTTTTGTGTA+AGG | + | chr8.3:18905714-18905733 | MS.gene73000:intron | 20.0% |
| !!! | ATCAAATCATTTAGTTTACT+AGG | + | chr8.3:18906130-18906149 | MS.gene73000:intron | 20.0% |
| !!! | TTGAAAGAAATGTTTAGAAT+TGG | - | chr8.3:18905307-18905326 | None:intergenic | 20.0% |
| ! | ACAAATACAGTTCATGAATT+GGG | - | chr8.3:18905920-18905939 | None:intergenic | 25.0% |
| ! | ATAATTATGCACATAGGTAT+AGG | + | chr8.3:18905570-18905589 | MS.gene73000:intron | 25.0% |
| ! | TACAAATACAGTTCATGAAT+TGG | - | chr8.3:18905921-18905940 | None:intergenic | 25.0% |
| !! | AAATGTGGTTTAGTTTATGA+TGG | + | chr8.3:18905506-18905525 | MS.gene73000:intron | 25.0% |
| !! | AGAAATGTTTAGAATTGGAT+TGG | - | chr8.3:18905302-18905321 | None:intergenic | 25.0% |
| !! | TCTAGGTGGTTTAATATTTT+GGG | + | chr8.3:18905868-18905887 | MS.gene73000:intron | 25.0% |
| !! | TTCTAGGTGGTTTAATATTT+TGG | + | chr8.3:18905867-18905886 | MS.gene73000:intron | 25.0% |
| AAGGAATAAACCAATCGAAA+TGG | - | chr8.3:18905684-18905703 | None:intergenic | 30.0% | |
| GTTCTTCTATGTTGTAAATG+TGG | + | chr8.3:18905491-18905510 | MS.gene73000:intron | 30.0% | |
| ! | CTAGGTGGTTTAATATTTTG+GGG | + | chr8.3:18905869-18905888 | MS.gene73000:intron | 30.0% |
| ! | TTTAGAATTGGATTGGATGA+TGG | - | chr8.3:18905295-18905314 | None:intergenic | 30.0% |
| ! | TTTCTGTGAATTTTGTGGAA+AGG | + | chr8.3:18906071-18906090 | MS.gene73000:CDS | 30.0% |
| !! | CAGAACTCAACATTTTGAAA+AGG | - | chr8.3:18906166-18906185 | None:intergenic | 30.0% |
| AACAACAGTTAATGTCACGT+GGG | - | chr8.3:18905643-18905662 | None:intergenic | 35.0% | |
| ACCTAGAATGTAACTACCTA+GGG | - | chr8.3:18905855-18905874 | None:intergenic | 35.0% | |
| ATGATATTAGGTACCCGTTA+TGG | + | chr8.3:18905994-18906013 | MS.gene73000:intron | 35.0% | |
| CATACACTACCTTACACAAA+AGG | - | chr8.3:18905726-18905745 | None:intergenic | 35.0% | |
| GCGTAAGCAGATTAAAAAGA+TGG | + | chr8.3:18906026-18906045 | MS.gene73000:CDS | 35.0% | |
| TGAACTGTATTTGTAGAGCA+AGG | + | chr8.3:18905926-18905945 | MS.gene73000:intron | 35.0% | |
| TGCACATAGGTATAGGTATA+TGG | + | chr8.3:18905577-18905596 | MS.gene73000:intron | 35.0% | |
| ACTAAGAGAACAAAGAAGGC+TGG | + | chr8.3:18905404-18905423 | MS.gene73000:CDS | 40.0% | |
| AGAAAGGCTGTAGGAATATG+GGG | + | chr8.3:18906219-18906238 | MS.gene73000:CDS | 40.0% | |
| AGAGAAAGGCTGTAGGAATA+TGG | + | chr8.3:18906217-18906236 | MS.gene73000:CDS | 40.0% | |
| CAACAACAGTTAATGTCACG+TGG | - | chr8.3:18905644-18905663 | None:intergenic | 40.0% | |
| CACCTAGAATGTAACTACCT+AGG | - | chr8.3:18905856-18905875 | None:intergenic | 40.0% | |
| CTAGGTAGTTACATTCTAGG+TGG | + | chr8.3:18905854-18905873 | MS.gene73000:intron | 40.0% | |
| GAGAAAGGCTGTAGGAATAT+GGG | + | chr8.3:18906218-18906237 | MS.gene73000:CDS | 40.0% | |
| GCAGACTAAGAGAACAAAGA+AGG | + | chr8.3:18905400-18905419 | MS.gene73000:intron | 40.0% | |
| TACGCAAACTAGCACCATAA+CGG | - | chr8.3:18906011-18906030 | None:intergenic | 40.0% | |
| TGTATAGCGACCATTTCGAT+TGG | + | chr8.3:18905671-18905690 | MS.gene73000:intron | 40.0% | |
| !! | ATTGGATTGGATGATGGAGA+AGG | - | chr8.3:18905289-18905308 | None:intergenic | 40.0% |
| ACGCAAACTAGCACCATAAC+GGG | - | chr8.3:18906010-18906029 | None:intergenic | 45.0% | |
| CAGTTAATGTCACGTGGGTT+CGG | - | chr8.3:18905638-18905657 | None:intergenic | 45.0% | |
| CTTCATAAGCCCATCCAAAC+AGG | + | chr8.3:18905829-18905848 | MS.gene73000:intron | 45.0% | |
| GCAGTATGCTGTGAAGAGAA+AGG | + | chr8.3:18906203-18906222 | MS.gene73000:intron | 45.0% | |
| GCCCTAGGTAGTTACATTCT+AGG | + | chr8.3:18905851-18905870 | MS.gene73000:intron | 45.0% | |
| TGTAGGAATATGGGGATGCA+AGG | + | chr8.3:18906227-18906246 | MS.gene73000:CDS | 45.0% | |
| ACAAAGAAGGCTGGCATCGT+TGG | + | chr8.3:18905413-18905432 | MS.gene73000:CDS | 50.0% | |
| ATATGGGGATGCAAGGACTG+CGG | + | chr8.3:18906234-18906253 | MS.gene73000:CDS | 50.0% | |
| GACTGCGGTAAAGTGAAAGC+AGG | + | chr8.3:18906249-18906268 | MS.gene73000:CDS | 50.0% | |
| GCTGGCATCGTTGGCAAATA+CGG | + | chr8.3:18905422-18905441 | MS.gene73000:CDS | 50.0% | |
| GCTGTGAAGAGAAAGGCTGT+AGG | + | chr8.3:18906210-18906229 | MS.gene73000:CDS | 50.0% | |
| GTAACTACCTAGGGCCTGTT+TGG | - | chr8.3:18905846-18905865 | None:intergenic | 50.0% | |
| TACCTAGGGCCTGTTTGGAT+GGG | - | chr8.3:18905841-18905860 | None:intergenic | 50.0% | |
| TGCGGTAAAGTGAAAGCAGG+CGG | + | chr8.3:18906252-18906271 | MS.gene73000:CDS | 50.0% | |
| CTACCTAGGGCCTGTTTGGA+TGG | - | chr8.3:18905842-18905861 | None:intergenic | 55.0% | |
| ! | GTCACGTGGGTTCGGTTACA+TGG | - | chr8.3:18905630-18905649 | None:intergenic | 55.0% |
| AGCCCATCCAAACAGGCCCT+AGG | + | chr8.3:18905836-18905855 | MS.gene73000:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 18905270 | 18906293 | 18905270 | ID=MS.gene73000 |
| chr8.3 | mRNA | 18905270 | 18906293 | 18905270 | ID=MS.gene73000.t1;Parent=MS.gene73000 |
| chr8.3 | exon | 18905270 | 18905272 | 18905270 | ID=MS.gene73000.t1.exon1;Parent=MS.gene73000.t1 |
| chr8.3 | CDS | 18905270 | 18905272 | 18905270 | ID=cds.MS.gene73000.t1;Parent=MS.gene73000.t1 |
| chr8.3 | exon | 18905404 | 18905443 | 18905404 | ID=MS.gene73000.t1.exon2;Parent=MS.gene73000.t1 |
| chr8.3 | CDS | 18905404 | 18905443 | 18905404 | ID=cds.MS.gene73000.t1;Parent=MS.gene73000.t1 |
| chr8.3 | exon | 18906004 | 18906092 | 18906004 | ID=MS.gene73000.t1.exon3;Parent=MS.gene73000.t1 |
| chr8.3 | CDS | 18906004 | 18906092 | 18906004 | ID=cds.MS.gene73000.t1;Parent=MS.gene73000.t1 |
| chr8.3 | exon | 18906207 | 18906293 | 18906207 | ID=MS.gene73000.t1.exon4;Parent=MS.gene73000.t1 |
| chr8.3 | CDS | 18906207 | 18906293 | 18906207 | ID=cds.MS.gene73000.t1;Parent=MS.gene73000.t1 |
| Gene Sequence |
| Protein sequence |
