Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73140.t1 | KEH19492.1 | 81.8 | 159 | 10 | 3 | 78 | 217 | 42 | 200 | 4.90E-65 | 257.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73140.t1 | A0A072TQ21 | 81.8 | 159 | 10 | 3 | 78 | 217 | 42 | 200 | 3.5e-65 | 257.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene73140 | 0.832027 | 1.19E-55 | -1.69E-46 |
| MS.gene050605 | MS.gene73140 | 0.821289 | 4.37E-53 | -1.69E-46 |
| MS.gene050750 | MS.gene73140 | 0.803029 | 4.24E-49 | -1.69E-46 |
| MS.gene050953 | MS.gene73140 | 0.814105 | 1.83E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene73140 | 0.805454 | 1.32E-49 | -1.69E-46 |
| MS.gene052863 | MS.gene73140 | 0.809673 | 1.68E-50 | -1.69E-46 |
| MS.gene053166 | MS.gene73140 | 0.81053 | 1.10E-50 | -1.69E-46 |
| MS.gene054236 | MS.gene73140 | 0.816897 | 4.36E-52 | -1.69E-46 |
| MS.gene054405 | MS.gene73140 | 0.806697 | 7.25E-50 | -1.69E-46 |
| MS.gene054467 | MS.gene73140 | 0.802729 | 4.89E-49 | -1.69E-46 |
| MS.gene054559 | MS.gene73140 | 0.810638 | 1.04E-50 | -1.69E-46 |
| MS.gene05510 | MS.gene73140 | 0.813268 | 2.79E-51 | -1.69E-46 |
| MS.gene055818 | MS.gene73140 | 0.817871 | 2.63E-52 | -1.69E-46 |
| MS.gene05604 | MS.gene73140 | 0.823942 | 1.06E-53 | -1.69E-46 |
| MS.gene057394 | MS.gene73140 | 0.804842 | 1.78E-49 | -1.69E-46 |
| MS.gene057444 | MS.gene73140 | 0.819839 | 9.41E-53 | -1.69E-46 |
| MS.gene057855 | MS.gene73140 | 0.803968 | 2.71E-49 | -1.69E-46 |
| MS.gene057869 | MS.gene73140 | 0.8053 | 1.43E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene73140 | 0.814521 | 1.48E-51 | -1.69E-46 |
| MS.gene058146 | MS.gene73140 | 0.816985 | 4.17E-52 | -1.69E-46 |
| MS.gene058595 | MS.gene73140 | 0.800669 | 1.29E-48 | -1.69E-46 |
| MS.gene058979 | MS.gene73140 | 0.825063 | 5.75E-54 | -1.69E-46 |
| MS.gene059128 | MS.gene73140 | 0.811044 | 8.52E-51 | -1.69E-46 |
| MS.gene059161 | MS.gene73140 | 0.807189 | 5.71E-50 | -1.69E-46 |
| MS.gene059550 | MS.gene73140 | 0.813875 | 2.05E-51 | -1.69E-46 |
| MS.gene060011 | MS.gene73140 | 0.80528 | 1.44E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene73140 | 0.820717 | 5.92E-53 | -1.69E-46 |
| MS.gene060322 | MS.gene73140 | 0.802619 | 5.15E-49 | -1.69E-46 |
| MS.gene060998 | MS.gene73140 | 0.83566 | 1.47E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73140.t1 | MTR_8g461300 | 83.854 | 192 | 12 | 3 | 45 | 217 | 9 | 200 | 5.25e-113 | 321 |
| MS.gene73140.t1 | MTR_4g055800 | 50.299 | 167 | 69 | 6 | 59 | 215 | 130 | 292 | 4.35e-41 | 141 |
| MS.gene73140.t1 | MTR_3g080680 | 40.606 | 165 | 69 | 8 | 71 | 215 | 43 | 198 | 1.34e-20 | 85.9 |
| MS.gene73140.t1 | MTR_3g082420 | 33.333 | 219 | 112 | 8 | 28 | 215 | 6 | 221 | 3.05e-14 | 69.3 |
| MS.gene73140.t1 | MTR_3g083030 | 30.455 | 220 | 110 | 10 | 28 | 215 | 21 | 229 | 2.24e-11 | 61.6 |
| MS.gene73140.t1 | MTR_3g083080 | 34.659 | 176 | 80 | 11 | 59 | 215 | 46 | 205 | 6.87e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 57 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTTAAAAGTAATGTAATA+TGG | 0.262097 | 3.4:+51131990 | None:intergenic |
| GATTTCAGCCGTTTCGATTT+CGG | 0.264712 | 3.4:+51132734 | None:intergenic |
| TGGTTCTTTCTATACGAATA+AGG | 0.287403 | 3.4:-51132495 | MS.gene73140:CDS |
| GAATCTTCTGGATCCGGTTT+CGG | 0.312704 | 3.4:+51132596 | None:intergenic |
| TCACTATACTCAGAATCTTC+TGG | 0.339930 | 3.4:+51132584 | None:intergenic |
| AAATCGAAACGGCTGAAATC+AGG | 0.356942 | 3.4:-51132731 | MS.gene73140:CDS |
| TCACGACGCTTATACCAATA+TGG | 0.364498 | 3.4:+51132518 | None:intergenic |
| ATGAAACACTCTTTGACAAC+AGG | 0.409120 | 3.4:+51131891 | None:intergenic |
| GTGTTTCAAAATGTCGAAAT+CGG | 0.413339 | 3.4:-51132765 | None:intergenic |
| GCTGAAATCGGAACCGAAAC+CGG | 0.414648 | 3.4:-51132609 | MS.gene73140:CDS |
| CTACTTCTTTATATGCTCCT+AGG | 0.435411 | 3.4:-51132013 | MS.gene73140:CDS |
| TACTCAGAATCTTCTGGATC+CGG | 0.439841 | 3.4:+51132590 | None:intergenic |
| TGCAGAGGGGTTTGAGAGTA+CGG | 0.440533 | 3.4:+51132216 | None:intergenic |
| GGAGGAGAAACGGCTGAAAT+CGG | 0.450703 | 3.4:-51132621 | MS.gene73140:CDS |
| TTCATCTGTGGAGGAGGAGG+AGG | 0.455235 | 3.4:-51132639 | MS.gene73140:CDS |
| CAAAATGTCGAAATCGGAAC+AGG | 0.457893 | 3.4:-51132759 | None:intergenic |
| CACTGTCCACCACGTTGTGC+TGG | 0.467693 | 3.4:-51132275 | MS.gene73140:CDS |
| TACGCTGCAATACATGAGTC+TGG | 0.467899 | 3.4:+51131925 | None:intergenic |
| AAAGTAATGTAATATGGCCT+AGG | 0.487724 | 3.4:+51131996 | None:intergenic |
| CAATCAAACAAATATTAAGC+AGG | 0.490074 | 3.4:-51132174 | MS.gene73140:intron |
| TAAAGCAAAAGCAGAAGCAA+AGG | 0.500294 | 3.4:-51131973 | MS.gene73140:CDS |
| CTTTCTATACGAATAAGGCT+TGG | 0.501112 | 3.4:-51132490 | MS.gene73140:CDS |
| TGAGGATGAGGAGGATAAGC+AGG | 0.508361 | 3.4:-51132678 | MS.gene73140:CDS |
| TCTATACGAATAAGGCTTGG+AGG | 0.522410 | 3.4:-51132487 | MS.gene73140:CDS |
| AGATTCATCTGTGGAGGAGG+AGG | 0.522683 | 3.4:-51132642 | MS.gene73140:CDS |
| GTCTGGAAAGTTGTTAGAGC+AGG | 0.523457 | 3.4:+51131942 | None:intergenic |
| GTTGTCAAGTGTACCCATCC+TGG | 0.532074 | 3.4:-51132038 | MS.gene73140:intron |
| AAAGCAAAAGCAGAAGCAAA+GGG | 0.538089 | 3.4:-51131972 | MS.gene73140:CDS |
| ACACGGTGAAGGTTCATATG+AGG | 0.543417 | 3.4:-51132696 | MS.gene73140:CDS |
| CTATACGAATAAGGCTTGGA+GGG | 0.544609 | 3.4:-51132486 | MS.gene73140:CDS |
| ACAAGATTCATCTGTGGAGG+AGG | 0.546974 | 3.4:-51132645 | MS.gene73140:CDS |
| CACATCATCGCTTAAGTCGA+GGG | 0.551344 | 3.4:+51132241 | None:intergenic |
| TAAGGCTTGGAGGGATCGTA+AGG | 0.553542 | 3.4:-51132477 | MS.gene73140:CDS |
| AACAGGAACCGAAATCGAAA+CGG | 0.558710 | 3.4:-51132742 | MS.gene73140:CDS |
| CTTCAAAGCAGCATTGCAGA+GGG | 0.559702 | 3.4:+51132202 | None:intergenic |
| TGAAGGTTCATATGAGGATG+AGG | 0.567019 | 3.4:-51132690 | MS.gene73140:CDS |
| GCATATAAAGAAGTAGCACC+AGG | 0.567371 | 3.4:+51132020 | None:intergenic |
| TGTCCACCACGTTGTGCTGG+CGG | 0.572730 | 3.4:-51132272 | MS.gene73140:CDS |
| AGGTTCATATGAGGATGAGG+AGG | 0.573297 | 3.4:-51132687 | MS.gene73140:CDS |
| TATTGGTATAAGCGTCGTGA+TGG | 0.589918 | 3.4:-51132515 | MS.gene73140:CDS |
| TAAAGAAGTAGCACCAGGAT+GGG | 0.590468 | 3.4:+51132025 | None:intergenic |
| GCACATCATCGCTTAAGTCG+AGG | 0.599300 | 3.4:+51132240 | None:intergenic |
| ATAAAGAAGTAGCACCAGGA+TGG | 0.610411 | 3.4:+51132024 | None:intergenic |
| CAGCACAACGTGGTGGACAG+TGG | 0.610706 | 3.4:+51132276 | None:intergenic |
| GAAACAAGATTCATCTGTGG+AGG | 0.613961 | 3.4:-51132648 | MS.gene73140:CDS |
| TCGGAACAGAAACACGGTGA+AGG | 0.629860 | 3.4:-51132707 | MS.gene73140:CDS |
| TCTTCAAAGCAGCATTGCAG+AGG | 0.630589 | 3.4:+51132201 | None:intergenic |
| AGAAGATTCTGAGTATAGTG+AGG | 0.652619 | 3.4:-51132582 | MS.gene73140:CDS |
| GCAAAAGCAGAAGCAAAGGG+AGG | 0.667232 | 3.4:-51131969 | MS.gene73140:CDS |
| GAAACGGCTGAAATCAGGAT+CGG | 0.671485 | 3.4:-51132726 | MS.gene73140:CDS |
| TGAGTATAGTGAGGAAGACG+AGG | 0.671624 | 3.4:-51132573 | MS.gene73140:CDS |
| CATGAAACAAGATTCATCTG+TGG | 0.677002 | 3.4:-51132651 | MS.gene73140:CDS |
| ATACCGCCAGCACAACGTGG+TGG | 0.689012 | 3.4:+51132269 | None:intergenic |
| ACATCATCGCTTAAGTCGAG+GGG | 0.690101 | 3.4:+51132242 | None:intergenic |
| TTCAAAGCAGCATTGCAGAG+GGG | 0.703047 | 3.4:+51132203 | None:intergenic |
| TCAGGATCGGAACAGAAACA+CGG | 0.709336 | 3.4:-51132713 | MS.gene73140:CDS |
| ACAATACCGCCAGCACAACG+TGG | 0.758364 | 3.4:+51132266 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GCTTTAAAAGTAATGTAATA+TGG | + | chr3.4:51132646-51132665 | None:intergenic | 20.0% |
| ! | ACTTTATCAAACACAAAAGT+TGG | + | chr3.4:51132577-51132596 | None:intergenic | 25.0% |
| ! | CAATCAAACAAATATTAAGC+AGG | - | chr3.4:51132459-51132478 | MS.gene73140:CDS | 25.0% |
| !!! | CACTTAATTTTGATTGATTC+TGG | - | chr3.4:51132306-51132325 | MS.gene73140:intron | 25.0% |
| AAAGTAATGTAATATGGCCT+AGG | + | chr3.4:51132640-51132659 | None:intergenic | 30.0% | |
| CAAACAAATATTAAGCAGGT+TGG | - | chr3.4:51132463-51132482 | MS.gene73140:CDS | 30.0% | |
| TGGTTCTTTCTATACGAATA+AGG | - | chr3.4:51132138-51132157 | MS.gene73140:intron | 30.0% | |
| ! | ACAAAGACAGTTTTCCATAT+TGG | - | chr3.4:51132101-51132120 | MS.gene73140:intron | 30.0% |
| AAAGCAAAAGCAGAAGCAAA+GGG | - | chr3.4:51132661-51132680 | MS.gene73140:CDS | 35.0% | |
| AGAAGATTCTGAGTATAGTG+AGG | - | chr3.4:51132051-51132070 | MS.gene73140:intron | 35.0% | |
| AGCAAACTAAGTGTAAGTGT+AGG | - | chr3.4:51132208-51132227 | MS.gene73140:CDS | 35.0% | |
| ATGAAACACTCTTTGACAAC+AGG | + | chr3.4:51132745-51132764 | None:intergenic | 35.0% | |
| CATGAAACAAGATTCATCTG+TGG | - | chr3.4:51131982-51132001 | MS.gene73140:CDS | 35.0% | |
| CTACTTCTTTATATGCTCCT+AGG | - | chr3.4:51132620-51132639 | MS.gene73140:CDS | 35.0% | |
| CTTTCTATACGAATAAGGCT+TGG | - | chr3.4:51132143-51132162 | MS.gene73140:intron | 35.0% | |
| TAAAGCAAAAGCAGAAGCAA+AGG | - | chr3.4:51132660-51132679 | MS.gene73140:CDS | 35.0% | |
| TCACTATACTCAGAATCTTC+TGG | + | chr3.4:51132052-51132071 | None:intergenic | 35.0% | |
| AAATCGAAACGGCTGAAATC+AGG | - | chr3.4:51131902-51131921 | MS.gene73140:CDS | 40.0% | |
| AACAGGAACCGAAATCGAAA+CGG | - | chr3.4:51131891-51131910 | MS.gene73140:CDS | 40.0% | |
| ATAAAGAAGTAGCACCAGGA+TGG | + | chr3.4:51132612-51132631 | None:intergenic | 40.0% | |
| CTATACGAATAAGGCTTGGA+GGG | - | chr3.4:51132147-51132166 | MS.gene73140:intron | 40.0% | |
| GAAACAAGATTCATCTGTGG+AGG | - | chr3.4:51131985-51132004 | MS.gene73140:CDS | 40.0% | |
| GATTCTGGACAATACAAAGC+AGG | - | chr3.4:51132321-51132340 | MS.gene73140:intron | 40.0% | |
| GATTTCAGCCGTTTCGATTT+CGG | + | chr3.4:51131902-51131921 | None:intergenic | 40.0% | |
| GCATATAAAGAAGTAGCACC+AGG | + | chr3.4:51132616-51132635 | None:intergenic | 40.0% | |
| TAAAGAAGTAGCACCAGGAT+GGG | + | chr3.4:51132611-51132630 | None:intergenic | 40.0% | |
| TACTCAGAATCTTCTGGATC+CGG | + | chr3.4:51132046-51132065 | None:intergenic | 40.0% | |
| TCACGACGCTTATACCAATA+TGG | + | chr3.4:51132118-51132137 | None:intergenic | 40.0% | |
| TCTATACGAATAAGGCTTGG+AGG | - | chr3.4:51132146-51132165 | MS.gene73140:intron | 40.0% | |
| TGAAGGTTCATATGAGGATG+AGG | - | chr3.4:51131943-51131962 | MS.gene73140:CDS | 40.0% | |
| ! | TATTGGTATAAGCGTCGTGA+TGG | - | chr3.4:51132118-51132137 | MS.gene73140:intron | 40.0% |
| ACAAGATTCATCTGTGGAGG+AGG | - | chr3.4:51131988-51132007 | MS.gene73140:CDS | 45.0% | |
| ACACGGTGAAGGTTCATATG+AGG | - | chr3.4:51131937-51131956 | MS.gene73140:CDS | 45.0% | |
| ACATCATCGCTTAAGTCGAG+GGG | + | chr3.4:51132394-51132413 | None:intergenic | 45.0% | |
| AGGTTCATATGAGGATGAGG+AGG | - | chr3.4:51131946-51131965 | MS.gene73140:CDS | 45.0% | |
| CACATCATCGCTTAAGTCGA+GGG | + | chr3.4:51132395-51132414 | None:intergenic | 45.0% | |
| CTTCAAAGCAGCATTGCAGA+GGG | + | chr3.4:51132434-51132453 | None:intergenic | 45.0% | |
| GAAACGGCTGAAATCAGGAT+CGG | - | chr3.4:51131907-51131926 | MS.gene73140:CDS | 45.0% | |
| GAATCTTCTGGATCCGGTTT+CGG | + | chr3.4:51132040-51132059 | None:intergenic | 45.0% | |
| GTCTGGAAAGTTGTTAGAGC+AGG | + | chr3.4:51132694-51132713 | None:intergenic | 45.0% | |
| TACGCTGCAATACATGAGTC+TGG | + | chr3.4:51132711-51132730 | None:intergenic | 45.0% | |
| TCAGGATCGGAACAGAAACA+CGG | - | chr3.4:51131920-51131939 | MS.gene73140:CDS | 45.0% | |
| TCTTCAAAGCAGCATTGCAG+AGG | + | chr3.4:51132435-51132454 | None:intergenic | 45.0% | |
| TGAGTATAGTGAGGAAGACG+AGG | - | chr3.4:51132060-51132079 | MS.gene73140:intron | 45.0% | |
| TTCAAAGCAGCATTGCAGAG+GGG | + | chr3.4:51132433-51132452 | None:intergenic | 45.0% | |
| !! | TACTTATTTATTATTAATTA+GGG | - | chr3.4:51132550-51132569 | MS.gene73140:CDS | 5.0% |
| !! | TTACTTATTTATTATTAATT+AGG | - | chr3.4:51132549-51132568 | MS.gene73140:CDS | 5.0% |
| AGATTCATCTGTGGAGGAGG+AGG | - | chr3.4:51131991-51132010 | MS.gene73140:CDS | 50.0% | |
| GCAAAAGCAGAAGCAAAGGG+AGG | - | chr3.4:51132664-51132683 | MS.gene73140:CDS | 50.0% | |
| GCACATCATCGCTTAAGTCG+AGG | + | chr3.4:51132396-51132415 | None:intergenic | 50.0% | |
| GCTGAAATCGGAACCGAAAC+CGG | - | chr3.4:51132024-51132043 | MS.gene73140:CDS | 50.0% | |
| GGAGGAGAAACGGCTGAAAT+CGG | - | chr3.4:51132012-51132031 | MS.gene73140:CDS | 50.0% | |
| GTTGTCAAGTGTACCCATCC+TGG | - | chr3.4:51132595-51132614 | MS.gene73140:CDS | 50.0% | |
| TAAGGCTTGGAGGGATCGTA+AGG | - | chr3.4:51132156-51132175 | MS.gene73140:intron | 50.0% | |
| TCGGAACAGAAACACGGTGA+AGG | - | chr3.4:51131926-51131945 | MS.gene73140:CDS | 50.0% | |
| TGAGGATGAGGAGGATAAGC+AGG | - | chr3.4:51131955-51131974 | MS.gene73140:CDS | 50.0% | |
| TGCAGAGGGGTTTGAGAGTA+CGG | + | chr3.4:51132420-51132439 | None:intergenic | 50.0% | |
| ACAATACCGCCAGCACAACG+TGG | + | chr3.4:51132370-51132389 | None:intergenic | 55.0% | |
| TGGAGGAGGAGGAGGAGAAA+CGG | - | chr3.4:51132002-51132021 | MS.gene73140:CDS | 55.0% | |
| TTCATCTGTGGAGGAGGAGG+AGG | - | chr3.4:51131994-51132013 | MS.gene73140:CDS | 55.0% | |
| ATACCGCCAGCACAACGTGG+TGG | + | chr3.4:51132367-51132386 | None:intergenic | 60.0% | |
| CACTGTCCACCACGTTGTGC+TGG | - | chr3.4:51132358-51132377 | MS.gene73140:intron | 60.0% | |
| TGTCCACCACGTTGTGCTGG+CGG | - | chr3.4:51132361-51132380 | MS.gene73140:intron | 60.0% | |
| !! | CAGCACAACGTGGTGGACAG+TGG | + | chr3.4:51132360-51132379 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 51131878 | 51132777 | 51131878 | ID=MS.gene73140 |
| chr3.4 | mRNA | 51131878 | 51132777 | 51131878 | ID=MS.gene73140.t1;Parent=MS.gene73140 |
| chr3.4 | exon | 51132436 | 51132777 | 51132436 | ID=MS.gene73140.t1.exon1;Parent=MS.gene73140.t1 |
| chr3.4 | CDS | 51132436 | 51132777 | 51132436 | ID=cds.MS.gene73140.t1;Parent=MS.gene73140.t1 |
| chr3.4 | exon | 51132175 | 51132312 | 51132175 | ID=MS.gene73140.t1.exon2;Parent=MS.gene73140.t1 |
| chr3.4 | CDS | 51132175 | 51132312 | 51132175 | ID=cds.MS.gene73140.t1;Parent=MS.gene73140.t1 |
| chr3.4 | exon | 51131878 | 51132051 | 51131878 | ID=MS.gene73140.t1.exon3;Parent=MS.gene73140.t1 |
| chr3.4 | CDS | 51131878 | 51132051 | 51131878 | ID=cds.MS.gene73140.t1;Parent=MS.gene73140.t1 |
| Gene Sequence |
| Protein sequence |
