Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73270.t1 | XP_003604820.1 | 97.5 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 6.30E-91 | 343.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73270.t1 | G7JRA4 | 97.5 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 4.5e-91 | 343.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene73270 | 0.807721 | 4.40E-50 | -1.69E-46 |
| MS.gene049419 | MS.gene73270 | -0.804746 | 1.86E-49 | -1.69E-46 |
| MS.gene049424 | MS.gene73270 | 0.811463 | 6.91E-51 | -1.69E-46 |
| MS.gene049653 | MS.gene73270 | 0.821556 | 3.79E-53 | -1.69E-46 |
| MS.gene050271 | MS.gene73270 | 0.821336 | 4.26E-53 | -1.69E-46 |
| MS.gene051122 | MS.gene73270 | 0.821302 | 4.34E-53 | -1.69E-46 |
| MS.gene052108 | MS.gene73270 | 0.807731 | 4.38E-50 | -1.69E-46 |
| MS.gene052500 | MS.gene73270 | 0.818726 | 1.69E-52 | -1.69E-46 |
| MS.gene052502 | MS.gene73270 | 0.825382 | 4.83E-54 | -1.69E-46 |
| MS.gene052824 | MS.gene73270 | 0.821572 | 3.76E-53 | -1.69E-46 |
| MS.gene052826 | MS.gene73270 | 0.822706 | 2.05E-53 | -1.69E-46 |
| MS.gene052954 | MS.gene73270 | 0.831536 | 1.58E-55 | -1.69E-46 |
| MS.gene053058 | MS.gene73270 | 0.804884 | 1.74E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene73270 | 0.825836 | 3.77E-54 | -1.69E-46 |
| MS.gene053298 | MS.gene73270 | 0.806929 | 6.48E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene73270 | 0.821592 | 3.72E-53 | -1.69E-46 |
| MS.gene053879 | MS.gene73270 | 0.803393 | 3.56E-49 | -1.69E-46 |
| MS.gene053963 | MS.gene73270 | 0.83886 | 2.24E-57 | -1.69E-46 |
| MS.gene054536 | MS.gene73270 | 0.821506 | 3.89E-53 | -1.69E-46 |
| MS.gene054893 | MS.gene73270 | 0.801539 | 8.58E-49 | -1.69E-46 |
| MS.gene055222 | MS.gene73270 | 0.801406 | 9.14E-49 | -1.69E-46 |
| MS.gene055226 | MS.gene73270 | 0.806649 | 7.43E-50 | -1.69E-46 |
| MS.gene055841 | MS.gene73270 | 0.804657 | 1.94E-49 | -1.69E-46 |
| MS.gene056787 | MS.gene73270 | 0.815592 | 8.54E-52 | -1.69E-46 |
| MS.gene056849 | MS.gene73270 | 0.824766 | 6.76E-54 | -1.69E-46 |
| MS.gene056975 | MS.gene73270 | 0.805974 | 1.03E-49 | -1.69E-46 |
| MS.gene057392 | MS.gene73270 | 0.803404 | 3.54E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene73270 | 0.835279 | 1.84E-56 | -1.69E-46 |
| MS.gene059872 | MS.gene73270 | -0.827547 | 1.47E-54 | -1.69E-46 |
| MS.gene060297 | MS.gene73270 | 0.81964 | 1.04E-52 | -1.69E-46 |
| MS.gene060840 | MS.gene73270 | 0.80748 | 4.95E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73270.t1 | MTR_4g019130 | 97.512 | 201 | 5 | 0 | 1 | 201 | 1 | 201 | 1.61e-141 | 393 |
| MS.gene73270.t1 | MTR_4g019150 | 73.786 | 206 | 45 | 3 | 1 | 201 | 1 | 202 | 1.85e-105 | 301 |
| MS.gene73270.t1 | MTR_7g104650 | 37.500 | 200 | 107 | 10 | 11 | 201 | 102 | 292 | 5.73e-25 | 99.0 |
| MS.gene73270.t1 | MTR_2g054130 | 36.979 | 192 | 95 | 6 | 11 | 201 | 211 | 377 | 2.39e-21 | 90.5 |
| MS.gene73270.t1 | MTR_2g055010 | 36.517 | 178 | 87 | 5 | 11 | 187 | 211 | 363 | 1.50e-18 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene73270.t1 | AT2G37970 | 60.648 | 216 | 69 | 3 | 1 | 201 | 32 | 246 | 3.05e-89 | 262 |
| MS.gene73270.t1 | AT5G20140 | 33.508 | 191 | 104 | 5 | 11 | 201 | 206 | 373 | 2.02e-20 | 87.8 |
| MS.gene73270.t1 | AT3G10130 | 37.000 | 200 | 108 | 10 | 11 | 201 | 115 | 305 | 2.82e-19 | 84.0 |
| MS.gene73270.t1 | AT5G20140 | 33.333 | 177 | 95 | 5 | 11 | 187 | 206 | 359 | 5.60e-18 | 81.3 |
Find 39 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGACAAAGATGGTGGGTTTA+AGG | 0.180691 | 4.1:-79109265 | MS.gene73270:CDS |
| TTTGATATGGGTTTAGTATT+TGG | 0.199696 | 4.1:-79109408 | None:intergenic |
| GAGGAAGGTGGGAAGAAATA+TGG | 0.218011 | 4.1:-79109003 | MS.gene73270:CDS |
| TTGTCTCCTTTGAATGTTGA+TGG | 0.274828 | 4.1:+79109282 | None:intergenic |
| AAATATGGGGTGGTGACTTT+TGG | 0.302611 | 4.1:-79108988 | MS.gene73270:CDS |
| TTCTTCTCATTTGTTACAAC+TGG | 0.303094 | 4.1:+79109117 | None:intergenic |
| CGATTACAATTCCAATGTTT+AGG | 0.308020 | 4.1:-79108849 | MS.gene73270:CDS |
| AGGAAGGTGGGAAGAAATAT+GGG | 0.347201 | 4.1:-79109002 | MS.gene73270:CDS |
| ATCCATCACGTTCATTCAAC+AGG | 0.374063 | 4.1:+79108808 | None:intergenic |
| GGAGAAGTTGAGGTTGTGTT+TGG | 0.422907 | 4.1:-79108929 | MS.gene73270:CDS |
| TTTGAATGTTGATGGATCAT+AGG | 0.424014 | 4.1:+79109290 | None:intergenic |
| ATCACAACCCTCTCATCAAT+AGG | 0.466452 | 4.1:+79109030 | None:intergenic |
| TTGTGATAAGAGAGGAAGGT+GGG | 0.471919 | 4.1:-79109014 | MS.gene73270:CDS |
| AGGGTTGTGATAAGAGAGGA+AGG | 0.486753 | 4.1:-79109018 | MS.gene73270:CDS |
| CTCATTTGTTACAACTGGTA+CGG | 0.490347 | 4.1:+79109122 | None:intergenic |
| AGGAGAAGGTGGAGAAGTTG+AGG | 0.492065 | 4.1:-79108939 | MS.gene73270:CDS |
| TCCAATGTTTAGGACTAATG+AGG | 0.513944 | 4.1:-79108839 | MS.gene73270:CDS |
| AGGTTGTGTTTGGAGAAAGA+TGG | 0.524733 | 4.1:-79108919 | MS.gene73270:CDS |
| TGAGAGGGTTGTGATAAGAG+AGG | 0.529346 | 4.1:-79109022 | MS.gene73270:CDS |
| TATGATCCATCAACATTCAA+AGG | 0.540575 | 4.1:-79109288 | MS.gene73270:CDS |
| AACATTGGAATTGTAATCGC+AGG | 0.545246 | 4.1:+79108853 | None:intergenic |
| ACCTAAGCCTATTGATGAGA+GGG | 0.548573 | 4.1:-79109037 | MS.gene73270:CDS |
| ACCCTCTCATCAATAGGCTT+AGG | 0.549333 | 4.1:+79109036 | None:intergenic |
| TGAAGTGGTGAAGGAGAAGG+TGG | 0.561858 | 4.1:-79108950 | MS.gene73270:CDS |
| ATTGGAATTGTAATCGCAGG+TGG | 0.568955 | 4.1:+79108856 | None:intergenic |
| ACCTCATTAGTCCTAAACAT+TGG | 0.572004 | 4.1:+79108838 | None:intergenic |
| GGCAAATGATGAAGTGGTGA+AGG | 0.590348 | 4.1:-79108959 | MS.gene73270:CDS |
| TTCAAAGGAGACAAAGATGG+TGG | 0.597630 | 4.1:-79109273 | MS.gene73270:CDS |
| GTGATTTCAGCTGCTAGAGA+AGG | 0.597735 | 4.1:+79109312 | None:intergenic |
| ACATTCAAAGGAGACAAAGA+TGG | 0.611112 | 4.1:-79109276 | MS.gene73270:CDS |
| TACCTAAGCCTATTGATGAG+AGG | 0.615966 | 4.1:-79109038 | MS.gene73270:CDS |
| TGATGAAGTGGTGAAGGAGA+AGG | 0.619951 | 4.1:-79108953 | MS.gene73270:CDS |
| AGGAGTGGCAAATGATGAAG+TGG | 0.629210 | 4.1:-79108965 | MS.gene73270:CDS |
| TCAAAGGAGACAAAGATGGT+GGG | 0.635974 | 4.1:-79109272 | MS.gene73270:CDS |
| AACAAATGAGAAGAACAAGA+TGG | 0.636525 | 4.1:-79109109 | MS.gene73270:CDS |
| AGGTGGGAAGAAATATGGGG+TGG | 0.665542 | 4.1:-79108998 | MS.gene73270:CDS |
| GTTGTGATAAGAGAGGAAGG+TGG | 0.671478 | 4.1:-79109015 | MS.gene73270:CDS |
| GACTTCTCCAGTTATCACAA+AGG | 0.674539 | 4.1:-79109178 | MS.gene73270:CDS |
| GGAAGGTGGGAAGAAATATG+GGG | 0.684324 | 4.1:-79109001 | MS.gene73270:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GTTTTGAGTATTTCATATTT+TGG | + | chr4.1:79108858-79108877 | None:intergenic | 20.0% |
| ! | TTCTATGTATGAAAAAGTAG+AGG | - | chr4.1:79109157-79109176 | MS.gene73270:CDS | 25.0% |
| !! | GTTGATTACATAGGAATTTT+TGG | - | chr4.1:79108984-79109003 | MS.gene73270:CDS | 25.0% |
| !!! | AAAGATGGTTTTAAGGTTAT+TGG | - | chr4.1:79109317-79109336 | MS.gene73270:CDS | 25.0% |
| !!! | AAGGTTTTAGTTGATTACAT+AGG | - | chr4.1:79108975-79108994 | MS.gene73270:CDS | 25.0% |
| !!! | GTTATTGGAGATTTTTTGTT+GGG | - | chr4.1:79109332-79109351 | MS.gene73270:CDS | 25.0% |
| !!! | TCTACTTTTTCATACATAGA+AGG | + | chr4.1:79109158-79109177 | None:intergenic | 25.0% |
| !!! | TTTTGTGTTTTTGTGTTTTG+TGG | + | chr4.1:79109014-79109033 | None:intergenic | 25.0% |
| AACAAATGAGAAGAACAAGA+TGG | - | chr4.1:79109112-79109131 | MS.gene73270:CDS | 30.0% | |
| CGATTACAATTCCAATGTTT+AGG | - | chr4.1:79109372-79109391 | MS.gene73270:CDS | 30.0% | |
| TATGATCCATCAACATTCAA+AGG | - | chr4.1:79108933-79108952 | MS.gene73270:CDS | 30.0% | |
| ! | TTCTTCTCATTTGTTACAAC+TGG | + | chr4.1:79109107-79109126 | None:intergenic | 30.0% |
| ! | TTGTTTTCCTTTGTGATAAC+TGG | + | chr4.1:79109053-79109072 | None:intergenic | 30.0% |
| ! | TTTGAATGTTGATGGATCAT+AGG | + | chr4.1:79108934-79108953 | None:intergenic | 30.0% |
| !! | TTTGGAGAAAGATGGTTTTA+AGG | - | chr4.1:79109310-79109329 | MS.gene73270:CDS | 30.0% |
| !!! | GGTTATTGGAGATTTTTTGT+TGG | - | chr4.1:79109331-79109350 | MS.gene73270:CDS | 30.0% |
| AACATTGGAATTGTAATCGC+AGG | + | chr4.1:79109371-79109390 | None:intergenic | 35.0% | |
| ACATTCAAAGGAGACAAAGA+TGG | - | chr4.1:79108945-79108964 | MS.gene73270:CDS | 35.0% | |
| ACCTCATTAGTCCTAAACAT+TGG | + | chr4.1:79109386-79109405 | None:intergenic | 35.0% | |
| TTGTCTCCTTTGAATGTTGA+TGG | + | chr4.1:79108942-79108961 | None:intergenic | 35.0% | |
| ! | CTCATTTGTTACAACTGGTA+CGG | + | chr4.1:79109102-79109121 | None:intergenic | 35.0% |
| ! | TCCAATGTTTAGGACTAATG+AGG | - | chr4.1:79109382-79109401 | MS.gene73270:CDS | 35.0% |
| !! | CTAGAGAAGGTGCATATTTT+CGG | + | chr4.1:79108899-79108918 | None:intergenic | 35.0% |
| !!! | TCGGATTACATAGTTTTGTG+TGG | + | chr4.1:79108880-79108899 | None:intergenic | 35.0% |
| !!! | TTGGAGATTTTTTGTTGGGT+AGG | - | chr4.1:79109336-79109355 | MS.gene73270:CDS | 35.0% |
| ACCTAAGCCTATTGATGAGA+GGG | - | chr4.1:79109184-79109203 | MS.gene73270:CDS | 40.0% | |
| AGGAAGGTGGGAAGAAATAT+GGG | - | chr4.1:79109219-79109238 | MS.gene73270:CDS | 40.0% | |
| AGGTTGTGTTTGGAGAAAGA+TGG | - | chr4.1:79109302-79109321 | MS.gene73270:CDS | 40.0% | |
| ATCACAACCCTCTCATCAAT+AGG | + | chr4.1:79109194-79109213 | None:intergenic | 40.0% | |
| ATTGGAATTGTAATCGCAGG+TGG | + | chr4.1:79109368-79109387 | None:intergenic | 40.0% | |
| GACTTCTCCAGTTATCACAA+AGG | - | chr4.1:79109043-79109062 | MS.gene73270:CDS | 40.0% | |
| TACCTAAGCCTATTGATGAG+AGG | - | chr4.1:79109183-79109202 | MS.gene73270:CDS | 40.0% | |
| TCAAAGGAGACAAAGATGGT+GGG | - | chr4.1:79108949-79108968 | MS.gene73270:CDS | 40.0% | |
| TTCAAAGGAGACAAAGATGG+TGG | - | chr4.1:79108948-79108967 | MS.gene73270:CDS | 40.0% | |
| TTGTGATAAGAGAGGAAGGT+GGG | - | chr4.1:79109207-79109226 | MS.gene73270:CDS | 40.0% | |
| ! | AAATATGGGGTGGTGACTTT+TGG | - | chr4.1:79109233-79109252 | MS.gene73270:CDS | 40.0% |
| !! | AGACAAAGATGGTGGGTTTA+AGG | - | chr4.1:79108956-79108975 | MS.gene73270:CDS | 40.0% |
| ACCCTCTCATCAATAGGCTT+AGG | + | chr4.1:79109188-79109207 | None:intergenic | 45.0% | |
| AGGAGTGGCAAATGATGAAG+TGG | - | chr4.1:79109256-79109275 | MS.gene73270:CDS | 45.0% | |
| AGGGTTGTGATAAGAGAGGA+AGG | - | chr4.1:79109203-79109222 | MS.gene73270:CDS | 45.0% | |
| GAGGAAGGTGGGAAGAAATA+TGG | - | chr4.1:79109218-79109237 | MS.gene73270:CDS | 45.0% | |
| GGAAGGTGGGAAGAAATATG+GGG | - | chr4.1:79109220-79109239 | MS.gene73270:CDS | 45.0% | |
| GGAGAAGTTGAGGTTGTGTT+TGG | - | chr4.1:79109292-79109311 | MS.gene73270:CDS | 45.0% | |
| GGCAAATGATGAAGTGGTGA+AGG | - | chr4.1:79109262-79109281 | MS.gene73270:CDS | 45.0% | |
| GTGATTTCAGCTGCTAGAGA+AGG | + | chr4.1:79108912-79108931 | None:intergenic | 45.0% | |
| GTTGTGATAAGAGAGGAAGG+TGG | - | chr4.1:79109206-79109225 | MS.gene73270:CDS | 45.0% | |
| TGAGAGGGTTGTGATAAGAG+AGG | - | chr4.1:79109199-79109218 | MS.gene73270:CDS | 45.0% | |
| TGATGAAGTGGTGAAGGAGA+AGG | - | chr4.1:79109268-79109287 | MS.gene73270:CDS | 45.0% | |
| AGGAGAAGGTGGAGAAGTTG+AGG | - | chr4.1:79109282-79109301 | MS.gene73270:CDS | 50.0% | |
| AGGTGGGAAGAAATATGGGG+TGG | - | chr4.1:79109223-79109242 | MS.gene73270:CDS | 50.0% | |
| TGAAGTGGTGAAGGAGAAGG+TGG | - | chr4.1:79109271-79109290 | MS.gene73270:CDS | 50.0% | |
| ! | TATGGGGTGGTGACTTTTGG+AGG | - | chr4.1:79109236-79109255 | MS.gene73270:CDS | 50.0% |
| !! | GGTGGTGACTTTTGGAGGAG+TGG | - | chr4.1:79109241-79109260 | MS.gene73270:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 79108819 | 79109424 | 79108819 | ID=MS.gene73270 |
| chr4.1 | mRNA | 79108819 | 79109424 | 79108819 | ID=MS.gene73270.t1;Parent=MS.gene73270 |
| chr4.1 | exon | 79108819 | 79109424 | 79108819 | ID=MS.gene73270.t1.exon1;Parent=MS.gene73270.t1 |
| chr4.1 | CDS | 79108819 | 79109424 | 79108819 | ID=cds.MS.gene73270.t1;Parent=MS.gene73270.t1 |
| Gene Sequence |
| Protein sequence |
