Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74674.t1 | XP_013451663.1 | 98.1 | 263 | 4 | 1 | 1 | 263 | 1 | 262 | 1.00E-144 | 523.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74674.t1 | Q8GW31 | 78.5 | 260 | 54 | 1 | 6 | 263 | 5 | 264 | 1.2e-117 | 424.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74674.t1 | A0A072U8J7 | 98.1 | 263 | 4 | 1 | 1 | 263 | 1 | 262 | 7.3e-145 | 523.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene74674 | 0.808835 | 2.55E-50 | -1.69E-46 |
| MS.gene049419 | MS.gene74674 | -0.801863 | 7.37E-49 | -1.69E-46 |
| MS.gene049435 | MS.gene74674 | 0.800649 | 1.31E-48 | -1.69E-46 |
| MS.gene050105 | MS.gene74674 | 0.81315 | 2.96E-51 | -1.69E-46 |
| MS.gene050559 | MS.gene74674 | 0.800039 | 1.74E-48 | -1.69E-46 |
| MS.gene050561 | MS.gene74674 | 0.800076 | 1.71E-48 | -1.69E-46 |
| MS.gene051800 | MS.gene74674 | 0.800349 | 1.50E-48 | -1.69E-46 |
| MS.gene052108 | MS.gene74674 | 0.804745 | 1.86E-49 | -1.69E-46 |
| MS.gene052333 | MS.gene74674 | 0.806706 | 7.22E-50 | -1.69E-46 |
| MS.gene052954 | MS.gene74674 | 0.820091 | 8.24E-53 | -1.69E-46 |
| MS.gene053166 | MS.gene74674 | 0.816077 | 6.66E-52 | -1.69E-46 |
| MS.gene05415 | MS.gene74674 | 0.821048 | 4.97E-53 | -1.69E-46 |
| MS.gene054236 | MS.gene74674 | 0.800184 | 1.62E-48 | -1.69E-46 |
| MS.gene054479 | MS.gene74674 | 0.813965 | 1.96E-51 | -1.69E-46 |
| MS.gene054657 | MS.gene74674 | 0.809793 | 1.59E-50 | -1.69E-46 |
| MS.gene054994 | MS.gene74674 | 0.801599 | 8.35E-49 | -1.69E-46 |
| MS.gene05510 | MS.gene74674 | 0.810366 | 1.19E-50 | -1.69E-46 |
| MS.gene055586 | MS.gene74674 | 0.800454 | 1.43E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene74674 | 0.823301 | 1.49E-53 | -1.69E-46 |
| MS.gene055841 | MS.gene74674 | 0.80723 | 5.60E-50 | -1.69E-46 |
| MS.gene056162 | MS.gene74674 | 0.81075 | 9.87E-51 | -1.69E-46 |
| MS.gene056506 | MS.gene74674 | 0.800865 | 1.18E-48 | -1.69E-46 |
| MS.gene056779 | MS.gene74674 | 0.800059 | 1.72E-48 | -1.69E-46 |
| MS.gene05686 | MS.gene74674 | 0.804828 | 1.79E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene74674 | 0.816502 | 5.35E-52 | -1.69E-46 |
| MS.gene057208 | MS.gene74674 | 0.81657 | 5.17E-52 | -1.69E-46 |
| MS.gene057598 | MS.gene74674 | 0.82521 | 5.31E-54 | -1.69E-46 |
| MS.gene057869 | MS.gene74674 | 0.803757 | 2.99E-49 | -1.69E-46 |
| MS.gene058010 | MS.gene74674 | 0.802366 | 5.81E-49 | -1.69E-46 |
| MS.gene058292 | MS.gene74674 | 0.801997 | 6.91E-49 | -1.69E-46 |
| MS.gene058979 | MS.gene74674 | 0.820295 | 7.40E-53 | -1.69E-46 |
| MS.gene05897 | MS.gene74674 | 0.803427 | 3.50E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene74674 | 0.803481 | 3.42E-49 | -1.69E-46 |
| MS.gene060935 | MS.gene74674 | 0.827716 | 1.34E-54 | -1.69E-46 |
| MS.gene060937 | MS.gene74674 | 0.820037 | 8.48E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74674.t1 | MTR_6g033535 | 98.099 | 263 | 4 | 1 | 1 | 263 | 1 | 262 | 0.0 | 529 |
| MS.gene74674.t1 | MTR_6g033535 | 98.305 | 236 | 4 | 0 | 28 | 263 | 15 | 250 | 8.35e-172 | 480 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74674.t1 | AT5G13570 | 80.162 | 247 | 49 | 0 | 17 | 263 | 18 | 264 | 5.75e-150 | 427 |
| MS.gene74674.t1 | AT5G13570 | 76.154 | 260 | 49 | 1 | 17 | 263 | 18 | 277 | 2.42e-146 | 419 |
Find 77 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTATATGGTGTCTCCCTTTC+TGG | 0.137033 | 6.3:+23218944 | MS.gene74674:CDS |
| AGACCTGATTTGCCACTTAA+AGG | 0.227358 | 6.3:+23219096 | MS.gene74674:CDS |
| AGAATTATTGCCCCAGTTAC+AGG | 0.269034 | 6.3:-23215404 | None:intergenic |
| AATGCAGATCTTTGAAATCT+TGG | 0.274871 | 6.3:+23219043 | MS.gene74674:intron |
| AAGGTTCGAGTTCCTGTAAC+TGG | 0.310426 | 6.3:+23215392 | MS.gene74674:CDS |
| CACTGGTCTTAAGCTGTATA+TGG | 0.326583 | 6.3:+23218929 | MS.gene74674:CDS |
| TTGACAGTGTTTGCTAGTTA+AGG | 0.332894 | 6.3:+23216359 | MS.gene74674:intron |
| ATTTGCCCCACAAACCAAAA+AGG | 0.347992 | 6.3:+23217334 | MS.gene74674:CDS |
| ATGCAGGTCTTGGAAGAAAC+TGG | 0.359933 | 6.3:+23217206 | MS.gene74674:intron |
| TGACAGTGTTTGCTAGTTAA+GGG | 0.368625 | 6.3:+23216360 | MS.gene74674:intron |
| ATACCTTTAAGTGGCAAATC+AGG | 0.382526 | 6.3:-23219099 | None:intergenic |
| TGCAAGAAATCTGCAACAAC+AGG | 0.388689 | 6.3:+23219580 | MS.gene74674:CDS |
| AATGATTGAAGATCCTCCTT+TGG | 0.413528 | 6.3:-23214380 | None:intergenic |
| CGAAAGGATCTTATTTCTTG+TGG | 0.433621 | 6.3:+23214403 | MS.gene74674:CDS |
| TTAAGTGGCAAATCAGGTCT+TGG | 0.443206 | 6.3:-23219093 | None:intergenic |
| AAAGAATTCCATGGTACCTT+GGG | 0.451920 | 6.3:-23219634 | None:intergenic |
| TCTTGATGAAACCTTTGAAA+GGG | 0.457863 | 6.3:+23215424 | MS.gene74674:CDS |
| GTACGACTCTACATTATTGC+TGG | 0.465038 | 6.3:+23217293 | MS.gene74674:CDS |
| AAGTGGGCACTTCTATGTAT+TGG | 0.468295 | 6.3:+23219678 | MS.gene74674:CDS |
| TTCAACACACCAACCTCCTA+TGG | 0.469327 | 6.3:+23219068 | MS.gene74674:CDS |
| GAGAAGACGGGGAATGTTAA+CGG | 0.471300 | 6.3:-23219300 | None:intergenic |
| GATATCACATCATCACTAGA+AGG | 0.473059 | 6.3:-23218897 | None:intergenic |
| AGGGATGGAAAGGTTCAAGT+TGG | 0.480500 | 6.3:+23216379 | MS.gene74674:CDS |
| AAAAGAATTCCATGGTACCT+TGG | 0.490131 | 6.3:-23219635 | None:intergenic |
| TCATCTATATGGGCCACATA+AGG | 0.495540 | 6.3:-23215344 | None:intergenic |
| TACAGGAACTCGAACCTTGT+AGG | 0.496480 | 6.3:-23215387 | None:intergenic |
| GTGATATCTCGTGGGATCAC+TGG | 0.499501 | 6.3:+23218912 | MS.gene74674:CDS |
| TTGGCACATTTAGCACAAAC+CGG | 0.502442 | 6.3:-23214361 | None:intergenic |
| TTCTCAGCTTACCCTTTCAA+AGG | 0.507737 | 6.3:-23215435 | None:intergenic |
| TAAGGACTTCACTTCCTACA+AGG | 0.509912 | 6.3:+23215373 | MS.gene74674:CDS |
| AACTCCTCGATGATCTCTGC+AGG | 0.514488 | 6.3:+23212519 | MS.gene74674:CDS |
| AAATTTCGACTGCTCTAAAG+TGG | 0.515341 | 6.3:+23219661 | MS.gene74674:CDS |
| ACATAGGAAATACCTTTAAG+TGG | 0.517044 | 6.3:-23219108 | None:intergenic |
| AGTTGTACTCACGCCAGAAA+GGG | 0.521072 | 6.3:-23218957 | None:intergenic |
| TGGTTATCACAATTAACTAC+AGG | 0.532346 | 6.3:+23219552 | MS.gene74674:CDS |
| TATCTTTACAGGAAATTGCA+TGG | 0.538158 | 6.3:+23218853 | MS.gene74674:intron |
| AGACTTAAAGACTTCAGAGA+TGG | 0.542098 | 6.3:-23214470 | None:intergenic |
| TTCTTGATGAAACCTTTGAA+AGG | 0.543629 | 6.3:+23215423 | MS.gene74674:CDS |
| TTGCTAGTTAAGGGATGGAA+AGG | 0.544440 | 6.3:+23216369 | MS.gene74674:CDS |
| TAACATTCCCCGTCTTCTCA+AGG | 0.544677 | 6.3:+23219304 | MS.gene74674:CDS |
| TTACACCACCTTGAGAAGAC+GGG | 0.544811 | 6.3:-23219312 | None:intergenic |
| AAATCAGGTCTTGGAGCCAT+AGG | 0.545316 | 6.3:-23219084 | None:intergenic |
| AGGTTCGAGTTCCTGTAACT+GGG | 0.545714 | 6.3:+23215393 | MS.gene74674:CDS |
| GGCTGAAGGCTACTGTACTC+TGG | 0.548324 | 6.3:+23219532 | MS.gene74674:CDS |
| AGTGTTTGCTAGTTAAGGGA+TGG | 0.553791 | 6.3:+23216364 | MS.gene74674:intron |
| TCTGAAGTCTTTAAGTCTGA+AGG | 0.553893 | 6.3:+23214475 | MS.gene74674:CDS |
| TGTTTCAAAATTTCTTAACA+AGG | 0.554360 | 6.3:+23217235 | MS.gene74674:CDS |
| AGATCATCGAGGAGTTCTTG+AGG | 0.556196 | 6.3:-23212512 | None:intergenic |
| GTCTTGGAGCCATAGGAGGT+TGG | 0.558406 | 6.3:-23219077 | None:intergenic |
| AAGTTGTACTCACGCCAGAA+AGG | 0.559904 | 6.3:-23218958 | None:intergenic |
| TGGGGTGCAGAACTGGATTG+TGG | 0.564634 | 6.3:+23219511 | MS.gene74674:intron |
| ATTAACAGAAAACTTAGTTG+TGG | 0.566593 | 6.3:+23219709 | MS.gene74674:CDS |
| CTTACACCACCTTGAGAAGA+CGG | 0.568909 | 6.3:-23219313 | None:intergenic |
| ATCACAATTAACTACAGGAC+TGG | 0.571086 | 6.3:+23219557 | MS.gene74674:CDS |
| GAGTTCTTGAGGAGGAAGAA+CGG | 0.571382 | 6.3:-23212501 | None:intergenic |
| GGCAGTACCCCTGTGATGAA+TGG | 0.582771 | 6.3:+23219608 | MS.gene74674:CDS |
| AGGATCTTCAATCATTCGAA+AGG | 0.590208 | 6.3:+23214387 | MS.gene74674:CDS |
| CTTGGGATACCATTCATCAC+AGG | 0.595684 | 6.3:-23219617 | None:intergenic |
| TTCTTGTGGAGTATGCACAT+TGG | 0.597459 | 6.3:+23214417 | MS.gene74674:CDS |
| TCAGGTCTTGGAGCCATAGG+AGG | 0.601393 | 6.3:-23219081 | None:intergenic |
| AATGGTATCCCAAGGTACCA+TGG | 0.611675 | 6.3:+23219626 | MS.gene74674:CDS |
| GTTTGTGCTAAATGTGCCAA+AGG | 0.613887 | 6.3:+23214364 | MS.gene74674:CDS |
| AATCTGCAACAACAGGGCTG+CGG | 0.617355 | 6.3:+23219587 | MS.gene74674:CDS |
| AATTTGTCAAAAGAATTCCA+TGG | 0.622118 | 6.3:-23219643 | None:intergenic |
| CTGTGATGAATGGTATCCCA+AGG | 0.625952 | 6.3:+23219618 | MS.gene74674:CDS |
| AGTGATGATGTGATATCTCG+TGG | 0.625983 | 6.3:+23218903 | MS.gene74674:CDS |
| TTGGGATACCATTCATCACA+GGG | 0.631833 | 6.3:-23219616 | None:intergenic |
| CAGAACTGGATTGTGGCTGA+AGG | 0.634881 | 6.3:+23219518 | MS.gene74674:intron |
| GGTTCGAGTTCCTGTAACTG+GGG | 0.643999 | 6.3:+23215394 | MS.gene74674:CDS |
| TCATCGAGGAGTTCTTGAGG+AGG | 0.644982 | 6.3:-23212509 | None:intergenic |
| TGTGCTAAATGTGCCAAAGG+AGG | 0.652756 | 6.3:+23214367 | MS.gene74674:CDS |
| GCAAGAAATCTGCAACAACA+GGG | 0.653602 | 6.3:+23219581 | MS.gene74674:CDS |
| AATTTCGACTGCTCTAAAGT+GGG | 0.655366 | 6.3:+23219662 | MS.gene74674:CDS |
| TACACCACCTTGAGAAGACG+GGG | 0.690247 | 6.3:-23219311 | None:intergenic |
| GTGATGATGTGATATCTCGT+GGG | 0.691549 | 6.3:+23218904 | MS.gene74674:CDS |
| CGAACCTGCAGAGATCATCG+AGG | 0.758435 | 6.3:-23212523 | None:intergenic |
| TGGGATACCATTCATCACAG+GGG | 0.788287 | 6.3:-23219615 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 23212458 | 23219739 | 23212458 | ID=MS.gene74674 |
| chr6.3 | mRNA | 23212458 | 23219739 | 23212458 | ID=MS.gene74674.t1;Parent=MS.gene74674 |
| chr6.3 | exon | 23212458 | 23212540 | 23212458 | ID=MS.gene74674.t1.exon1;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23212458 | 23212540 | 23212458 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23214361 | 23214512 | 23214361 | ID=MS.gene74674.t1.exon2;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23214361 | 23214512 | 23214361 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23215318 | 23215445 | 23215318 | ID=MS.gene74674.t1.exon3;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23215318 | 23215445 | 23215318 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23216366 | 23216461 | 23216366 | ID=MS.gene74674.t1.exon4;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23216366 | 23216461 | 23216366 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23217212 | 23217364 | 23217212 | ID=MS.gene74674.t1.exon5;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23217212 | 23217364 | 23217212 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23218864 | 23218967 | 23218864 | ID=MS.gene74674.t1.exon6;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23218864 | 23218967 | 23218864 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23219050 | 23219117 | 23219050 | ID=MS.gene74674.t1.exon7;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23219050 | 23219117 | 23219050 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23219264 | 23219325 | 23219264 | ID=MS.gene74674.t1.exon8;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23219264 | 23219325 | 23219264 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| chr6.3 | exon | 23219521 | 23219739 | 23219521 | ID=MS.gene74674.t1.exon9;Parent=MS.gene74674.t1 |
| chr6.3 | CDS | 23219521 | 23219739 | 23219521 | ID=cds.MS.gene74674.t1;Parent=MS.gene74674.t1 |
| Gene Sequence |
| Protein sequence |
