AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050832


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 0 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTAGATTAAATTATTATT+TGG - 50589:24197-24216 MS.gene050832:CDS 10.0%
!! ATACGAAATAATTACTAAAT+CGG + 50589:24404-24423 None:intergenic 15.0%
!! TGTCAACAAAAAAAAAAAAA+AGG - 50589:24577-24596 MS.gene050832:intron 15.0%
!! TACGAAATAATTACTAAATC+GGG + 50589:24403-24422 None:intergenic 20.0%
!! TGATTAAGTATAATATGGTT+GGG + 50589:24638-24657 None:intergenic 20.0%
!! TTGATTAAGTATAATATGGT+TGG + 50589:24639-24658 None:intergenic 20.0%
! ATATACTAAGTTGTTCAATG+TGG - 50589:25120-25139 MS.gene050832:CDS 25.0%
! TCGCTTGATTAAGTATAATA+TGG + 50589:24643-24662 None:intergenic 25.0%
! TTGTGTAGGAATTATAACTA+TGG - 50589:24812-24831 MS.gene050832:intron 25.0%
!!! CATAGAAACAATTTTAGGTT+TGG + 50589:24256-24275 None:intergenic 25.0%
AGCTAATGAGTTTCAGTTAA+AGG - 50589:24675-24694 MS.gene050832:intron 30.0%
CTACACAAAAGTTTATGAGA+GGG + 50589:24800-24819 None:intergenic 30.0%
GAATTGTTTGATACAATCTC+TGG + 50589:24878-24897 None:intergenic 30.0%
GTATACCAAGATAGTTGAAT+AGG + 50589:24153-24172 None:intergenic 30.0%
TACACAAAAGTTTATGAGAG+GGG + 50589:24799-24818 None:intergenic 30.0%
TGATATGCACAATCCAAATT+AGG + 50589:25154-25173 None:intergenic 30.0%
TGTGATGGTTGGAAAATATT+TGG - 50589:24967-24986 MS.gene050832:CDS 30.0%
TTTGAAGGCAAAAGTTACAA+AGG - 50589:24120-24139 MS.gene050832:CDS 30.0%
! AGGATCATATGGATTAAAAC+TGG + 50589:24334-24353 None:intergenic 30.0%
! GAGCTCATAGAAACAATTTT+AGG + 50589:24261-24280 None:intergenic 30.0%
! TTGGTGACAAATATAGTCAA+TGG - 50589:25034-25053 MS.gene050832:CDS 30.0%
! TTTGAAACTAATTTAGCGAC+CGG + 50589:24447-24466 None:intergenic 30.0%
!! AAATATAGTCAATGGTGGAT+TGG - 50589:25042-25061 MS.gene050832:CDS 30.0%
AAAACTTATTTCCCAAGCTG+AGG + 50589:24359-24378 None:intergenic 35.0%
AAGTTGTTCAATGTGGATAC+TGG - 50589:25127-25146 MS.gene050832:CDS 35.0%
AATTATAACTATGGACCAGC+AGG - 50589:24821-24840 MS.gene050832:intron 35.0%
AGCAGCGTTAAAATTCTTGA+CGG - 50589:24308-24327 MS.gene050832:intron 35.0%
ATTATAACTATGGACCAGCA+GGG - 50589:24822-24841 MS.gene050832:intron 35.0%
ATTTCACATGAAACTACTGG+TGG - 50589:23991-24010 MS.gene050832:CDS 35.0%
CAGATTTCACATGAAACTAC+TGG - 50589:23988-24007 MS.gene050832:CDS 35.0%
CCTACACAAAAGTTTATGAG+AGG + 50589:24801-24820 None:intergenic 35.0%
GCAAGAGTTAATGATCGAAT+TGG - 50589:25085-25104 MS.gene050832:CDS 35.0%
GTGACAAATATAGTCAATGG+TGG - 50589:25037-25056 MS.gene050832:CDS 35.0%
GTGATTCTACTGACAAAGAT+TGG - 50589:24091-24110 MS.gene050832:CDS 35.0%
TATTTCGTATCTGTAGTGAC+CGG - 50589:24414-24433 MS.gene050832:intron 35.0%
TCATTAACTCTTGCATCATC+AGG + 50589:25079-25098 None:intergenic 35.0%
TGTGGATTTGGACAAAGAAT+TGG + 50589:24230-24249 None:intergenic 35.0%
TTGCTTTCAAAACTGCTCTA+TGG - 50589:24903-24922 MS.gene050832:intron 35.0%
TTTCACATGAAACTACTGGT+GGG - 50589:23992-24011 MS.gene050832:CDS 35.0%
! ACATTGTGACAAGAAGGTAT+TGG + 50589:24950-24969 None:intergenic 35.0%
! CCTCTCATAAACTTTTGTGT+AGG - 50589:24798-24817 MS.gene050832:intron 35.0%
! GAATCACAGTAGTTACTTTG+AGG + 50589:24078-24097 None:intergenic 35.0%
! TAACTTTTGCCTTCAAAGCA+AGG + 50589:24117-24136 None:intergenic 35.0%
! TCAAAACTGCTCTATGGTTT+TGG - 50589:24909-24928 MS.gene050832:intron 35.0%
!!! CAATTTTAGGTTTGGATGTG+TGG + 50589:24248-24267 None:intergenic 35.0%
AAGCATAAGCCCCAAGAAAA+TGG + 50589:24039-24058 None:intergenic 40.0%
ACCATCACATTGTGACAAGA+AGG + 50589:24956-24975 None:intergenic 40.0%
ACCTTCTTGTCACAATGTGA+TGG - 50589:24952-24971 MS.gene050832:intron 40.0%
GAGGTCCTATTCAACTATCT+TGG - 50589:24145-24164 MS.gene050832:CDS 40.0%
GGCAAAAGTTACAAAGGAAG+AGG - 50589:24126-24145 MS.gene050832:CDS 40.0%
TAGGTTTGGATGTGTGGATT+TGG + 50589:24242-24261 None:intergenic 40.0%
TCTTGTCACAATGTGATGGT+TGG - 50589:24956-24975 MS.gene050832:intron 40.0%
TGTGGATACTGGACCTAATT+TGG - 50589:25138-25157 MS.gene050832:CDS 40.0%
TTTAGCGACCGGTTATAAAC+CGG + 50589:24436-24455 None:intergenic 40.0%
! TGTAGTGACCGGTTTATAAC+CGG - 50589:24425-24444 MS.gene050832:intron 40.0%
!! AAAGATTGGCCTTGCTTTGA+AGG - 50589:24105-24124 MS.gene050832:CDS 40.0%
!! ACCTGATGGACCATTTTCTT+GGG - 50589:24026-24045 MS.gene050832:CDS 40.0%
AAGCTGAGGTGAGGATCATA+TGG + 50589:24345-24364 None:intergenic 45.0%
AGCTATCTCACGCTTTCTTG+TGG + 50589:23956-23975 None:intergenic 45.0%
ATAGAAGCTAATCGCACAGC+TGG - 50589:25004-25023 MS.gene050832:CDS 45.0%
ATATGATCCTCACCTCAGCT+TGG - 50589:24344-24363 MS.gene050832:intron 45.0%
TATGATCCTCACCTCAGCTT+GGG - 50589:24345-24364 MS.gene050832:intron 45.0%
TTATTTCCCAAGCTGAGGTG+AGG + 50589:24354-24373 None:intergenic 45.0%
! ACATGAAACTACTGGTGGGT+GGG - 50589:23996-24015 MS.gene050832:CDS 45.0%
!! CACCTGATGGACCATTTTCT+TGG - 50589:24025-24044 MS.gene050832:CDS 45.0%
!! CCTGATGGACCATTTTCTTG+GGG - 50589:24027-24046 MS.gene050832:CDS 45.0%
!! GGAAGGCGTTAGGATTTGAT+GGG - 50589:24843-24862 MS.gene050832:intron 45.0%
!! GTCAATGGTGGATTGGAATG+TGG - 50589:25049-25068 MS.gene050832:CDS 45.0%
AAATGGTCCATCAGGTGCAG+TGG + 50589:24022-24041 None:intergenic 50.0%
CACATGAAACTACTGGTGGG+TGG - 50589:23995-24014 MS.gene050832:CDS 50.0%
CCCCAAGAAAATGGTCCATC+AGG + 50589:24030-24049 None:intergenic 50.0%
TAACTATGGACCAGCAGGGA+AGG - 50589:24826-24845 MS.gene050832:intron 50.0%
!! GCTAATCGCACAGCTGGTTT+TGG - 50589:25010-25029 MS.gene050832:CDS 50.0%
!! GGGAAGGCGTTAGGATTTGA+TGG - 50589:24842-24861 MS.gene050832:intron 50.0%
!!! TCGCACAGCTGGTTTTGGAT+TGG - 50589:25015-25034 MS.gene050832:CDS 50.0%
AATCCTAACGCCTTCCCTGC+TGG + 50589:24839-24858 None:intergenic 55.0%
!! GGACCAGCAGGGAAGGCGTT+AGG - 50589:24833-24852 MS.gene050832:intron 65.0%
GGGTGGGCCACTGCACCTGA+TGG - 50589:24012-24031 MS.gene050832:CDS 70.0%


Chromosome Type Strat End Strand Name
50589 gene 23944 25186 23944 ID=MS.gene050832
50589 mRNA 23944 25186 23944 ID=MS.gene050832.t1;Parent=MS.gene050832
50589 exon 24964 25186 24964 ID=MS.gene050832.t1.exon1;Parent=MS.gene050832.t1
50589 CDS 24964 25186 24964 ID=cds.MS.gene050832.t1;Parent=MS.gene050832.t1
50589 exon 23944 24310 23944 ID=MS.gene050832.t1.exon2;Parent=MS.gene050832.t1
50589 CDS 23944 24310 23944 ID=cds.MS.gene050832.t1;Parent=MS.gene050832.t1
Gene Sequence

>MS.gene050832

GTTGTTTGACCACAAGAAAGCGTGAGATAGCTGCTTTTCTTGCTCAGATTTCACATGAAACTACTGGTGGGTGGGCCACTGCACCTGATGGACCATTTTCTTGGGGCTTATGCTTCAAAGAAGAAATTAGTCCTCAAAGTAACTACTGTGATTCTACTGACAAAGATTGGCCTTGCTTTGAAGGCAAAAGTTACAAAGGAAGAGGTCCTATTCAACTATCTTGGTATACACTAACTACTTGCAAACTATTGATTCTTAGATTAAATTATTATTTGGTTTATTACCAATTCTTTGTCCAAATCCACACATCCAAACCTAAAATTGTTTCTATGAGCTCAAATTCAATATGTTATAGTGTACGTATAGCAGCGTTAAAATTCTTGACGGCCAGTTTTAATCCATATGATCCTCACCTCAGCTTGGGAAATAAGTTTTCTGAAAATTTGCTATGTAGAACCCGATTTAGTAATTATTTCGTATCTGTAGTGACCGGTTTATAACCGGTCGCTAAATTAGTTTCAAAATTAGCTGCAAAATGAGATTGCAGTCGATTTTTTTTTTTTTTAGAGACTAATTTGCAAAAAAAAAAAACAACTAGACATCTAAATCATTCTCTAACTATATCAGTTTATTTGTCAACAAAAAAAAAAAAAAGGAGAAACTCATTTATGTCAAACAAAATATTCACACACCCAACCATATTATACTTAATCAAGCGATTGAATTCAAGTAGCTAATGAGTTTCAGTTAAAGGCAGTTTGTTCGAGAGAATTCAAGTTCAAATTCTAACTAAAACTATAATAGTAAAATTTTACTTGCGTCTTAATCAATCGAACTCAAGATTAACAGAGTCCCCTCTCATAAACTTTTGTGTAGGAATTATAACTATGGACCAGCAGGGAAGGCGTTAGGATTTGATGGGCTGAGAAATCCAGAGATTGTATCAAACAATTCAGTGATTGCTTTCAAAACTGCTCTATGGTTTTGGATGACAGAGAGAAAACCAATACCTTCTTGTCACAATGTGATGGTTGGAAAATATTTGGCAACAAAAGCTGACATAGAAGCTAATCGCACAGCTGGTTTTGGATTGGTGACAAATATAGTCAATGGTGGATTGGAATGTGGAATTCCTGATGATGCAAGAGTTAATGATCGAATTGGATTTTTTCAAAGATATACTAAGTTGTTCAATGTGGATACTGGACCTAATTTGGATTGTGCATATCAGAAATCTTTTTAA

Protein sequence

>MS.gene050832.t1

VVUPQESVRULLFLLRFHMKLLVGGPLHLMDHFLGAYASKKKLVLKVTTVILLTKIGLALKAKVTKEEVLFNYLGIITMDQQGRRUDLMGUEIQRLYQTIQULLSKLLYGFGUQRENQYLLVTMUWLENIWQQKLTUKLIAQLVLDWUQIUSMVDWNVEFLMMQELMIELDFFKDILSCSMWILDLIWIVHIRNLF