Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77731.t1 | XP_013455324.1 | 91.5 | 47 | 4 | 0 | 21 | 67 | 150 | 196 | 1.30E-16 | 95.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77731.t1 | F4KDA6 | 62.8 | 43 | 16 | 0 | 21 | 63 | 139 | 181 | 1.7e-09 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77731.t1 | A0A072UU36 | 91.5 | 47 | 4 | 0 | 21 | 67 | 150 | 196 | 9.3e-17 | 95.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050301 | MS.gene77731 | 0.808674 | 2.76E-50 | -1.69E-46 |
| MS.gene050397 | MS.gene77731 | 0.811745 | 6.00E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene77731 | 0.826377 | 2.81E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene77731 | 0.812355 | 4.42E-51 | -1.69E-46 |
| MS.gene051525 | MS.gene77731 | 0.810943 | 8.97E-51 | -1.69E-46 |
| MS.gene051582 | MS.gene77731 | 0.825117 | 5.58E-54 | -1.69E-46 |
| MS.gene051860 | MS.gene77731 | 0.807761 | 4.32E-50 | -1.69E-46 |
| MS.gene052011 | MS.gene77731 | -0.806367 | 8.51E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene77731 | 0.812014 | 5.25E-51 | -1.69E-46 |
| MS.gene052481 | MS.gene77731 | 0.806337 | 8.64E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene77731 | 0.800889 | 1.17E-48 | -1.69E-46 |
| MS.gene053836 | MS.gene77731 | 0.808331 | 3.26E-50 | -1.69E-46 |
| MS.gene055116 | MS.gene77731 | 0.823162 | 1.61E-53 | -1.69E-46 |
| MS.gene055277 | MS.gene77731 | 0.818407 | 1.99E-52 | -1.69E-46 |
| MS.gene05553 | MS.gene77731 | 0.81318 | 2.92E-51 | -1.69E-46 |
| MS.gene055564 | MS.gene77731 | 0.80254 | 5.35E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene77731 | -0.804578 | 2.02E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene77731 | 0.801805 | 7.57E-49 | -1.69E-46 |
| MS.gene058002 | MS.gene77731 | 0.824772 | 6.74E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene77731 | 0.816407 | 5.62E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene77731 | 0.80741 | 5.12E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene77731 | 0.806698 | 7.25E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene77731 | 0.823912 | 1.07E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene77731 | 0.821999 | 3.00E-53 | -1.69E-46 |
| MS.gene059688 | MS.gene77731 | 0.814378 | 1.59E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene77731 | 0.801634 | 8.21E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene77731 | 0.805084 | 1.58E-49 | -1.69E-46 |
| MS.gene060403 | MS.gene77731 | 0.808562 | 2.91E-50 | -1.69E-46 |
| MS.gene061049 | MS.gene77731 | 0.817459 | 3.26E-52 | -1.69E-46 |
| MS.gene061159 | MS.gene77731 | 0.805017 | 1.64E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77731.t1 | MTR_4g035825 | 82.692 | 52 | 9 | 0 | 16 | 67 | 145 | 196 | 1.15e-26 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77731.t1 | AT5G51545 | 62.791 | 43 | 16 | 0 | 21 | 63 | 139 | 181 | 3.01e-14 | 63.5 |
Find 14 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAACTCTTCTTCTTCTTCA+TGG | 0.155370 | 4.4:-68661878 | None:intergenic |
| AGGAAATTGTCCCACTCTTC+TGG | 0.220374 | 4.4:-68662457 | None:intergenic |
| ACAGTATTATTGTTTCTTGC+TGG | 0.296795 | 4.4:+68662511 | MS.gene77731:CDS |
| CTTGCTGGAGCAGTAGCATT+TGG | 0.343726 | 4.4:+68662526 | MS.gene77731:CDS |
| CCATGAAGAAGAAGAAGAGT+TGG | 0.456160 | 4.4:+68661878 | None:intergenic |
| CAATAATACTGTAGGGGTAA+AGG | 0.457025 | 4.4:-68662500 | None:intergenic |
| ACTCTCGATATGTTGTCAAT+AGG | 0.472365 | 4.4:-68661913 | None:intergenic |
| GCAAGAAACAATAATACTGT+AGG | 0.502406 | 4.4:-68662508 | None:intergenic |
| CAAGAAACAATAATACTGTA+GGG | 0.504164 | 4.4:-68662507 | None:intergenic |
| AATACTGTAGGGGTAAAGGA+TGG | 0.613012 | 4.4:-68662496 | None:intergenic |
| TAAGCAGAATGAGAAACTCA+CGG | 0.646278 | 4.4:+68662561 | MS.gene77731:CDS |
| AAGAAACAATAATACTGTAG+GGG | 0.647405 | 4.4:-68662506 | None:intergenic |
| ATGGATAGAGATACTTGACA+AGG | 0.707055 | 4.4:-68662477 | None:intergenic |
| TGGAGCAGTAGCATTTGGAG+TGG | 0.720186 | 4.4:+68662531 | MS.gene77731:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATACTATTTATTATTCA+AGG | + | chr4.4:68662133-68662152 | MS.gene77731:intron | 10.0% |
| !!! | ATGTTCATTTAAAAATTTTG+AGG | + | chr4.4:68662178-68662197 | MS.gene77731:intron | 15.0% |
| !! | AGAAATGAAAAACATTAGTT+TGG | - | chr4.4:68662205-68662224 | None:intergenic | 20.0% |
| ! | AAGAAACAATAATACTGTAG+GGG | - | chr4.4:68662509-68662528 | None:intergenic | 25.0% |
| ! | CAAGAAACAATAATACTGTA+GGG | - | chr4.4:68662510-68662529 | None:intergenic | 25.0% |
| ! | GTAAAATGACACTAATTCTT+AGG | - | chr4.4:68662391-68662410 | None:intergenic | 25.0% |
| !!! | GGATTTATTTTTATTCTCTC+TGG | + | chr4.4:68661942-68661961 | MS.gene77731:intron | 25.0% |
| !!! | TAACTCGTTCTTTAGAATAA+AGG | - | chr4.4:68662280-68662299 | None:intergenic | 25.0% |
| CTGATTAAGATACAATAGCT+TGG | - | chr4.4:68662341-68662360 | None:intergenic | 30.0% | |
| GCAAGAAACAATAATACTGT+AGG | - | chr4.4:68662511-68662530 | None:intergenic | 30.0% | |
| TGGGAGGAAAAATAAAGTAT+CGG | + | chr4.4:68662029-68662048 | MS.gene77731:intron | 30.0% | |
| ! | ACAGTATTATTGTTTCTTGC+TGG | + | chr4.4:68662511-68662530 | MS.gene77731:CDS | 30.0% |
| ! | ACTCTGATACTTTCATTTTC+GGG | - | chr4.4:68662085-68662104 | None:intergenic | 30.0% |
| ! | CACTCTGATACTTTCATTTT+CGG | - | chr4.4:68662086-68662105 | None:intergenic | 30.0% |
| ! | CTTAATCAGTTAGTTGACTT+AGG | + | chr4.4:68662352-68662371 | MS.gene77731:intron | 30.0% |
| ACTCTCGATATGTTGTCAAT+AGG | - | chr4.4:68661916-68661935 | None:intergenic | 35.0% | |
| AGAGACTATCACGTACAATA+TGG | + | chr4.4:68662009-68662028 | MS.gene77731:intron | 35.0% | |
| ATGGATAGAGATACTTGACA+AGG | - | chr4.4:68662480-68662499 | None:intergenic | 35.0% | |
| CAATAATACTGTAGGGGTAA+AGG | - | chr4.4:68662503-68662522 | None:intergenic | 35.0% | |
| CGATACTGTGGTTAATACTT+TGG | + | chr4.4:68662413-68662432 | MS.gene77731:intron | 35.0% | |
| GACACTAATTCTTAGGTTCT+AGG | - | chr4.4:68662384-68662403 | None:intergenic | 35.0% | |
| GAGACTATCACGTACAATAT+GGG | + | chr4.4:68662010-68662029 | MS.gene77731:intron | 35.0% | |
| TAAGCAGAATGAGAAACTCA+CGG | + | chr4.4:68662561-68662580 | MS.gene77731:CDS | 35.0% | |
| ! | ATACTTTGGTGTGTTATTGC+AGG | + | chr4.4:68662427-68662446 | MS.gene77731:intron | 35.0% |
| !! | GTCATTTTACATCGATACTG+TGG | + | chr4.4:68662401-68662420 | MS.gene77731:intron | 35.0% |
| AATACTGTAGGGGTAAAGGA+TGG | - | chr4.4:68662499-68662518 | None:intergenic | 40.0% | |
| AATAGCTTGGAGACCATCTT+AGG | - | chr4.4:68662328-68662347 | None:intergenic | 40.0% | |
| ACTATCACGTACAATATGGG+AGG | + | chr4.4:68662013-68662032 | MS.gene77731:intron | 40.0% | |
| ACTATGACCCAAACCTAAGA+TGG | + | chr4.4:68662312-68662331 | MS.gene77731:intron | 40.0% | |
| TTGGAGACCATCTTAGGTTT+GGG | - | chr4.4:68662322-68662341 | None:intergenic | 40.0% | |
| ! | CAACATATCGAGAGTGTGTA+AGG | + | chr4.4:68661921-68661940 | MS.gene77731:intron | 40.0% |
| !!! | AGGCTTTTTTCCAGAAGAGT+GGG | + | chr4.4:68662447-68662466 | MS.gene77731:intron | 40.0% |
| AGGAAATTGTCCCACTCTTC+TGG | - | chr4.4:68662460-68662479 | None:intergenic | 45.0% | |
| CTTGGAGACCATCTTAGGTT+TGG | - | chr4.4:68662323-68662342 | None:intergenic | 45.0% | |
| !!! | CAGGCTTTTTTCCAGAAGAG+TGG | + | chr4.4:68662446-68662465 | MS.gene77731:intron | 45.0% |
| CTTGCTGGAGCAGTAGCATT+TGG | + | chr4.4:68662526-68662545 | MS.gene77731:CDS | 50.0% | |
| TGGAGCAGTAGCATTTGGAG+TGG | + | chr4.4:68662531-68662550 | MS.gene77731:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 68661880 | 68662594 | 68661880 | ID=MS.gene77731 |
| chr4.4 | mRNA | 68661880 | 68662594 | 68661880 | ID=MS.gene77731.t1;Parent=MS.gene77731 |
| chr4.4 | exon | 68661880 | 68661937 | 68661880 | ID=MS.gene77731.t1.exon1;Parent=MS.gene77731.t1 |
| chr4.4 | CDS | 68661880 | 68661937 | 68661880 | ID=cds.MS.gene77731.t1;Parent=MS.gene77731.t1 |
| chr4.4 | exon | 68662449 | 68662594 | 68662449 | ID=MS.gene77731.t1.exon2;Parent=MS.gene77731.t1 |
| chr4.4 | CDS | 68662449 | 68662594 | 68662449 | ID=cds.MS.gene77731.t1;Parent=MS.gene77731.t1 |
| Gene Sequence |
| Protein sequence |
