Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79222.t1 | XP_013461269.1 | 98 | 199 | 4 | 0 | 1 | 199 | 1 | 199 | 2.30E-93 | 351.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79222.t1 | A0A072UZT9 | 98.0 | 199 | 4 | 0 | 1 | 199 | 1 | 199 | 1.6e-93 | 351.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene79222 | -0.821156 | 4.69E-53 | -1.69E-46 |
| MS.gene049549 | MS.gene79222 | 0.805974 | 1.03E-49 | -1.69E-46 |
| MS.gene050612 | MS.gene79222 | -0.802479 | 5.50E-49 | -1.69E-46 |
| MS.gene050647 | MS.gene79222 | 0.805464 | 1.32E-49 | -1.69E-46 |
| MS.gene050720 | MS.gene79222 | 0.802488 | 5.48E-49 | -1.69E-46 |
| MS.gene051127 | MS.gene79222 | 0.806936 | 6.46E-50 | -1.69E-46 |
| MS.gene051138 | MS.gene79222 | 0.802078 | 6.65E-49 | -1.69E-46 |
| MS.gene051973 | MS.gene79222 | 0.8371 | 6.34E-57 | -1.69E-46 |
| MS.gene052211 | MS.gene79222 | 0.866588 | 2.55E-65 | -1.69E-46 |
| MS.gene052212 | MS.gene79222 | 0.82394 | 1.06E-53 | -1.69E-46 |
| MS.gene052606 | MS.gene79222 | 0.811318 | 7.43E-51 | -1.69E-46 |
| MS.gene054146 | MS.gene79222 | 0.81043 | 1.16E-50 | -1.69E-46 |
| MS.gene054421 | MS.gene79222 | 0.8277 | 1.35E-54 | -1.69E-46 |
| MS.gene05470 | MS.gene79222 | -0.809147 | 2.18E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene79222 | 0.815812 | 7.63E-52 | -1.69E-46 |
| MS.gene056218 | MS.gene79222 | 0.813434 | 2.56E-51 | -1.69E-46 |
| MS.gene056224 | MS.gene79222 | 0.800941 | 1.14E-48 | -1.69E-46 |
| MS.gene056590 | MS.gene79222 | 0.803904 | 2.79E-49 | -1.69E-46 |
| MS.gene056591 | MS.gene79222 | 0.803698 | 3.08E-49 | -1.69E-46 |
| MS.gene056692 | MS.gene79222 | -0.802598 | 5.20E-49 | -1.69E-46 |
| MS.gene056748 | MS.gene79222 | 0.827706 | 1.35E-54 | -1.69E-46 |
| MS.gene056988 | MS.gene79222 | 0.821952 | 3.07E-53 | -1.69E-46 |
| MS.gene057820 | MS.gene79222 | 0.812632 | 3.85E-51 | -1.69E-46 |
| MS.gene057831 | MS.gene79222 | -0.808378 | 3.19E-50 | -1.69E-46 |
| MS.gene058419 | MS.gene79222 | -0.802139 | 6.46E-49 | -1.69E-46 |
| MS.gene058544 | MS.gene79222 | -0.809474 | 1.86E-50 | -1.69E-46 |
| MS.gene058603 | MS.gene79222 | 0.823923 | 1.07E-53 | -1.69E-46 |
| MS.gene059022 | MS.gene79222 | -0.804661 | 1.94E-49 | -1.69E-46 |
| MS.gene059677 | MS.gene79222 | -0.806709 | 7.21E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene79222 | 0.814014 | 1.91E-51 | -1.69E-46 |
| MS.gene060087 | MS.gene79222 | 0.812475 | 4.16E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene79222 | -0.802651 | 5.07E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79222.t1 | MTR_3g087278 | 97.487 | 199 | 5 | 0 | 1 | 199 | 1 | 199 | 8.10e-145 | 401 |
| MS.gene79222.t1 | MTR_3g087270 | 88.945 | 199 | 22 | 0 | 1 | 199 | 1 | 199 | 7.38e-134 | 373 |
| MS.gene79222.t1 | MTR_3g087325 | 90.452 | 199 | 19 | 0 | 1 | 199 | 1 | 199 | 1.26e-133 | 372 |
| MS.gene79222.t1 | MTR_3g087280 | 87.940 | 199 | 24 | 0 | 1 | 199 | 1 | 199 | 2.47e-133 | 372 |
| MS.gene79222.t1 | MTR_3g087330 | 86.935 | 199 | 26 | 0 | 1 | 199 | 1 | 199 | 4.83e-130 | 363 |
| MS.gene79222.t1 | MTR_3g087275 | 88.442 | 199 | 22 | 1 | 1 | 199 | 1 | 198 | 2.91e-128 | 359 |
| MS.gene79222.t1 | MTR_3g087300 | 65.482 | 197 | 24 | 3 | 1 | 197 | 1 | 153 | 4.26e-86 | 253 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79222.t1 | AT5G65030 | 31.313 | 198 | 122 | 4 | 11 | 199 | 10 | 202 | 5.92e-25 | 97.1 |
| MS.gene79222.t1 | AT5G10210 | 31.443 | 194 | 109 | 7 | 15 | 196 | 13 | 194 | 1.70e-24 | 95.5 |
Find 41 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGACAGATTCAACGTTTC+GGG | 0.191083 | 3.1:+70246646 | None:intergenic |
| CCTCTAGGCTTTGTTGATTA+AGG | 0.216614 | 3.1:+70246471 | None:intergenic |
| CCACCAAATCAGTACCCTTT+TGG | 0.225754 | 3.1:-70246544 | MS.gene79222:CDS |
| ATAAGAGAAGGTTGAATAAT+TGG | 0.248389 | 3.1:-70246229 | MS.gene79222:CDS |
| TTAATTTCCACTTCCACTTC+TGG | 0.251842 | 3.1:+70246290 | None:intergenic |
| TGCGCTTGGTGCAGCTTTAG+AGG | 0.317354 | 3.1:-70246138 | MS.gene79222:CDS |
| CCAAGGTTGTATCTTCCAAC+AGG | 0.400612 | 3.1:-70246605 | MS.gene79222:CDS |
| AAAATGGTTGAAAATGGATT+TGG | 0.402081 | 3.1:-70246351 | MS.gene79222:CDS |
| ATCCCAAAAGGGTACTGATT+TGG | 0.405118 | 3.1:+70246541 | None:intergenic |
| ACTTGACAGATTCAACGTTT+CGG | 0.407723 | 3.1:+70246645 | None:intergenic |
| CGCAAATAAATCACAATCTT+CGG | 0.408596 | 3.1:+70246157 | None:intergenic |
| TCCACTTCCACTTCTGGTGT+TGG | 0.429577 | 3.1:+70246296 | None:intergenic |
| GATTCAAGAATCACTTTCCA+TGG | 0.437928 | 3.1:+70246392 | None:intergenic |
| ATTAAAAGACTCATCCCAAA+AGG | 0.445639 | 3.1:+70246529 | None:intergenic |
| TTTGCCTTAGCTCCACCTCT+AGG | 0.447483 | 3.1:+70246456 | None:intergenic |
| AGTGACTTCGGTTGCTGAGA+TGG | 0.471131 | 3.1:-70246261 | MS.gene79222:CDS |
| CAGCTGAAGTTTGCATAAGC+AGG | 0.474655 | 3.1:+70246689 | None:intergenic |
| TTGGCAAAGGTGAGATTCCA+TGG | 0.486019 | 3.1:-70246409 | MS.gene79222:CDS |
| CCAAAAGGGTACTGATTTGG+TGG | 0.487432 | 3.1:+70246544 | None:intergenic |
| GCATAAGCAGGTATATGTAA+TGG | 0.520758 | 3.1:+70246701 | None:intergenic |
| GATTGTGATTTATTTGCGCT+TGG | 0.539399 | 3.1:-70246152 | MS.gene79222:CDS |
| GCCAACACCAGAAGTGGAAG+TGG | 0.540390 | 3.1:-70246297 | MS.gene79222:CDS |
| TGGAAAAGAAGAATAAGAGA+AGG | 0.547734 | 3.1:-70246241 | MS.gene79222:CDS |
| AATTAAAATAAGAGTGACTT+CGG | 0.548743 | 3.1:-70246273 | MS.gene79222:CDS |
| TTGAAAATGGATTTGGTGAG+TGG | 0.556088 | 3.1:-70246344 | MS.gene79222:CDS |
| GCTAAGGCAAAGGAAGATGT+GGG | 0.557016 | 3.1:-70246444 | MS.gene79222:CDS |
| AGCTAAGGCAAAGGAAGATG+TGG | 0.560256 | 3.1:-70246445 | MS.gene79222:CDS |
| TTAAAAGACTCATCCCAAAA+GGG | 0.562396 | 3.1:+70246530 | None:intergenic |
| AAAGCCTAGAGGTGGAGCTA+AGG | 0.569801 | 3.1:-70246460 | MS.gene79222:CDS |
| TTGAATAATTGGAATGAGTG+TGG | 0.570637 | 3.1:-70246218 | MS.gene79222:CDS |
| CCTGTTGGAAGATACAACCT+TGG | 0.577511 | 3.1:+70246605 | None:intergenic |
| GATTCTTGAATCACAAAACA+TGG | 0.583125 | 3.1:-70246381 | MS.gene79222:CDS |
| TAGAGGTGGAGCTAAGGCAA+AGG | 0.590191 | 3.1:-70246454 | MS.gene79222:CDS |
| TATGTTGCCAACACCAGAAG+TGG | 0.592676 | 3.1:-70246303 | MS.gene79222:CDS |
| GGGTCACAACTTATTGGCAA+AGG | 0.622442 | 3.1:-70246422 | MS.gene79222:CDS |
| ATGGTCATGATCATAATGCT+TGG | 0.627084 | 3.1:-70246187 | MS.gene79222:CDS |
| TAATCAACAAAGCCTAGAGG+TGG | 0.629386 | 3.1:-70246468 | MS.gene79222:CDS |
| TAAGGCAAAGGAAGATGTGG+GGG | 0.660678 | 3.1:-70246442 | MS.gene79222:CDS |
| CTAAGGCAAAGGAAGATGTG+GGG | 0.689053 | 3.1:-70246443 | MS.gene79222:CDS |
| CCTTAATCAACAAAGCCTAG+AGG | 0.695556 | 3.1:-70246471 | MS.gene79222:CDS |
| GAGTGGAAGTGATTGCAAAG+AGG | 0.732454 | 3.1:-70246327 | MS.gene79222:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTAAAATAAGAGTGACTT+CGG | - | chr3.1:70246564-70246583 | MS.gene79222:CDS | 20.0% |
| ! | AAAACATGGAGCTAAAAAAA+TGG | - | chr3.1:70246470-70246489 | MS.gene79222:CDS | 25.0% |
| ! | ATAAGAGAAGGTTGAATAAT+TGG | - | chr3.1:70246608-70246627 | MS.gene79222:CDS | 25.0% |
| ! | GCTAAAAAAATGGTTGAAAA+TGG | - | chr3.1:70246480-70246499 | MS.gene79222:CDS | 25.0% |
| !! | AAAATGGTTGAAAATGGATT+TGG | - | chr3.1:70246486-70246505 | MS.gene79222:CDS | 25.0% |
| ATTAAAAGACTCATCCCAAA+AGG | + | chr3.1:70246311-70246330 | None:intergenic | 30.0% | |
| CGCAAATAAATCACAATCTT+CGG | + | chr3.1:70246683-70246702 | None:intergenic | 30.0% | |
| GATTCTTGAATCACAAAACA+TGG | - | chr3.1:70246456-70246475 | MS.gene79222:CDS | 30.0% | |
| TGGAAAAGAAGAATAAGAGA+AGG | - | chr3.1:70246596-70246615 | MS.gene79222:CDS | 30.0% | |
| TTAAAAGACTCATCCCAAAA+GGG | + | chr3.1:70246310-70246329 | None:intergenic | 30.0% | |
| TTGAATAATTGGAATGAGTG+TGG | - | chr3.1:70246619-70246638 | MS.gene79222:CDS | 30.0% | |
| ! | AAGGTTTTCCAAGAATTCTT+GGG | + | chr3.1:70246350-70246369 | None:intergenic | 30.0% |
| ! | TAAGGTTTTCCAAGAATTCT+TGG | + | chr3.1:70246351-70246370 | None:intergenic | 30.0% |
| !!! | ATTCTTTTGTTGTTTCCTGT+TGG | + | chr3.1:70246250-70246269 | None:intergenic | 30.0% |
| AATGAGTGTGGATGCAAAAA+TGG | - | chr3.1:70246631-70246650 | MS.gene79222:CDS | 35.0% | |
| ACTTGACAGATTCAACGTTT+CGG | + | chr3.1:70246195-70246214 | None:intergenic | 35.0% | |
| ATGGTCATGATCATAATGCT+TGG | - | chr3.1:70246650-70246669 | MS.gene79222:CDS | 35.0% | |
| GATTCAAGAATCACTTTCCA+TGG | + | chr3.1:70246448-70246467 | None:intergenic | 35.0% | |
| GATTGTGATTTATTTGCGCT+TGG | - | chr3.1:70246685-70246704 | MS.gene79222:CDS | 35.0% | |
| GCATAAGCAGGTATATGTAA+TGG | + | chr3.1:70246139-70246158 | None:intergenic | 35.0% | |
| TTAATTTCCACTTCCACTTC+TGG | + | chr3.1:70246550-70246569 | None:intergenic | 35.0% | |
| ! | AGGTTTTCCAAGAATTCTTG+GGG | + | chr3.1:70246349-70246368 | None:intergenic | 35.0% |
| !! | TTGAAAATGGATTTGGTGAG+TGG | - | chr3.1:70246493-70246512 | MS.gene79222:CDS | 35.0% |
| CCTTAATCAACAAAGCCTAG+AGG | - | chr3.1:70246366-70246385 | MS.gene79222:CDS | 40.0% | |
| CTTGACAGATTCAACGTTTC+GGG | + | chr3.1:70246194-70246213 | None:intergenic | 40.0% | |
| TAATCAACAAAGCCTAGAGG+TGG | - | chr3.1:70246369-70246388 | MS.gene79222:CDS | 40.0% | |
| ! | CACCAAATCAGTACCCTTTT+GGG | - | chr3.1:70246294-70246313 | MS.gene79222:CDS | 40.0% |
| ! | CCACGAAAAACTTGTTTCCA+AGG | - | chr3.1:70246215-70246234 | MS.gene79222:CDS | 40.0% |
| ! | CCTTGGAAACAAGTTTTTCG+TGG | + | chr3.1:70246218-70246237 | None:intergenic | 40.0% |
| ! | GGTTTTCCAAGAATTCTTGG+GGG | + | chr3.1:70246348-70246367 | None:intergenic | 40.0% |
| !! | ATCCCAAAAGGGTACTGATT+TGG | + | chr3.1:70246299-70246318 | None:intergenic | 40.0% |
| !! | CCTCTAGGCTTTGTTGATTA+AGG | + | chr3.1:70246369-70246388 | None:intergenic | 40.0% |
| AGCTAAGGCAAAGGAAGATG+TGG | - | chr3.1:70246392-70246411 | MS.gene79222:CDS | 45.0% | |
| ATGTGGGGGTCACAACTTAT+TGG | - | chr3.1:70246409-70246428 | MS.gene79222:CDS | 45.0% | |
| CAGCTGAAGTTTGCATAAGC+AGG | + | chr3.1:70246151-70246170 | None:intergenic | 45.0% | |
| CCAAGGTTGTATCTTCCAAC+AGG | - | chr3.1:70246232-70246251 | MS.gene79222:CDS | 45.0% | |
| CCTGTTGGAAGATACAACCT+TGG | + | chr3.1:70246235-70246254 | None:intergenic | 45.0% | |
| CTAAGGCAAAGGAAGATGTG+GGG | - | chr3.1:70246394-70246413 | MS.gene79222:CDS | 45.0% | |
| GAGTGGAAGTGATTGCAAAG+AGG | - | chr3.1:70246510-70246529 | MS.gene79222:CDS | 45.0% | |
| GCTAAGGCAAAGGAAGATGT+GGG | - | chr3.1:70246393-70246412 | MS.gene79222:CDS | 45.0% | |
| GGGTCACAACTTATTGGCAA+AGG | - | chr3.1:70246415-70246434 | MS.gene79222:CDS | 45.0% | |
| TAAGGCAAAGGAAGATGTGG+GGG | - | chr3.1:70246395-70246414 | MS.gene79222:CDS | 45.0% | |
| TATGTTGCCAACACCAGAAG+TGG | - | chr3.1:70246534-70246553 | MS.gene79222:CDS | 45.0% | |
| TTCTTGCCCCCAAGAATTCT+TGG | - | chr3.1:70246339-70246358 | MS.gene79222:CDS | 45.0% | |
| TTGGCAAAGGTGAGATTCCA+TGG | - | chr3.1:70246428-70246447 | MS.gene79222:CDS | 45.0% | |
| ! | CCACCAAATCAGTACCCTTT+TGG | - | chr3.1:70246293-70246312 | MS.gene79222:CDS | 45.0% |
| !! | CCAAAAGGGTACTGATTTGG+TGG | + | chr3.1:70246296-70246315 | None:intergenic | 45.0% |
| AAAGCCTAGAGGTGGAGCTA+AGG | - | chr3.1:70246377-70246396 | MS.gene79222:CDS | 50.0% | |
| AGTGACTTCGGTTGCTGAGA+TGG | - | chr3.1:70246576-70246595 | MS.gene79222:CDS | 50.0% | |
| TAGAGGTGGAGCTAAGGCAA+AGG | - | chr3.1:70246383-70246402 | MS.gene79222:CDS | 50.0% | |
| TCCACTTCCACTTCTGGTGT+TGG | + | chr3.1:70246544-70246563 | None:intergenic | 50.0% | |
| TTTGCCTTAGCTCCACCTCT+AGG | + | chr3.1:70246384-70246403 | None:intergenic | 50.0% | |
| GCCAACACCAGAAGTGGAAG+TGG | - | chr3.1:70246540-70246559 | MS.gene79222:CDS | 55.0% | |
| !! | TGCGCTTGGTGCAGCTTTAG+AGG | - | chr3.1:70246699-70246718 | MS.gene79222:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 70246130 | 70246729 | 70246130 | ID=MS.gene79222 |
| chr3.1 | mRNA | 70246130 | 70246729 | 70246130 | ID=MS.gene79222.t1;Parent=MS.gene79222 |
| chr3.1 | exon | 70246130 | 70246729 | 70246130 | ID=MS.gene79222.t1.exon1;Parent=MS.gene79222.t1 |
| chr3.1 | CDS | 70246130 | 70246729 | 70246130 | ID=cds.MS.gene79222.t1;Parent=MS.gene79222.t1 |
| Gene Sequence |
| Protein sequence |
