Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057820.t1 | XP_003607111.1 | 87.9 | 321 | 19 | 1 | 1 | 301 | 1 | 321 | 4.30E-160 | 573.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057820.t1 | E7C196 | 57.0 | 323 | 112 | 2 | 5 | 300 | 4 | 326 | 3.5e-102 | 372.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057820.t1 | G7JL33 | 87.9 | 321 | 19 | 1 | 1 | 301 | 1 | 321 | 3.1e-160 | 573.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049549 | MS.gene057820 | 0.807303 | 5.40E-50 | -1.69E-46 |
| MS.gene052211 | MS.gene057820 | 0.815873 | 7.39E-52 | -1.69E-46 |
| MS.gene05240 | MS.gene057820 | 0.807878 | 4.08E-50 | -1.69E-46 |
| MS.gene055240 | MS.gene057820 | 0.808499 | 3.01E-50 | -1.69E-46 |
| MS.gene056748 | MS.gene057820 | 0.821859 | 3.23E-53 | -1.69E-46 |
| MS.gene056920 | MS.gene057820 | 0.80838 | 3.19E-50 | -1.69E-46 |
| MS.gene057261 | MS.gene057820 | 0.800092 | 1.69E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene062180 | 0.800178 | 1.63E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene064583 | 0.806201 | 9.23E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene064807 | 0.815702 | 8.07E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene065729 | 0.802688 | 4.98E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene06593 | 0.823243 | 1.54E-53 | -1.69E-46 |
| MS.gene057820 | MS.gene066500 | 0.801766 | 7.71E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene066602 | 0.816182 | 6.31E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene06805 | 0.822492 | 2.30E-53 | -1.69E-46 |
| MS.gene057820 | MS.gene069482 | 0.814752 | 1.31E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene072293 | 0.82964 | 4.60E-55 | -1.69E-46 |
| MS.gene057820 | MS.gene073526 | 0.810075 | 1.38E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene07488 | 0.804657 | 1.94E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene08402 | 0.818561 | 1.84E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene24862 | 0.80101 | 1.10E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene24881 | 0.80032 | 1.52E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene26294 | 0.8133 | 2.74E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene26475 | 0.803434 | 3.49E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene27643 | 0.806363 | 8.53E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene28594 | 0.801813 | 7.54E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene28602 | 0.813244 | 2.82E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene30183 | 0.802866 | 4.58E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene31542 | 0.820204 | 7.76E-53 | -1.69E-46 |
| MS.gene057820 | MS.gene33445 | 0.803232 | 3.85E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene35597 | 0.818416 | 1.98E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene35598 | 0.801135 | 1.04E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene35741 | 0.840267 | 9.64E-58 | -1.69E-46 |
| MS.gene057820 | MS.gene37747 | 0.805879 | 1.08E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene37749 | 0.819162 | 1.34E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene44712 | 0.801268 | 9.76E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene48885 | 0.80209 | 6.62E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene49021 | 0.820146 | 8.00E-53 | -1.69E-46 |
| MS.gene057820 | MS.gene50678 | 0.804488 | 2.11E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene50679 | 0.861314 | 1.12E-63 | -1.69E-46 |
| MS.gene057820 | MS.gene52370 | 0.809485 | 1.85E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene58228 | 0.815221 | 1.03E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene59003 | 0.816667 | 4.91E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene60531 | 0.808329 | 3.27E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene61142 | 0.800204 | 1.61E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene62222 | 0.802009 | 6.88E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene63914 | 0.810621 | 1.05E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene64276 | 0.810757 | 9.84E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene65040 | 0.802276 | 6.06E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene65821 | 0.809137 | 2.20E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene69966 | 0.802605 | 5.18E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene70929 | 0.808101 | 3.65E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene70930 | 0.818259 | 2.15E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene71350 | 0.826473 | 2.66E-54 | -1.69E-46 |
| MS.gene057820 | MS.gene74786 | 0.807524 | 4.85E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene75472 | 0.805834 | 1.10E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene79222 | 0.812632 | 3.85E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene79517 | 0.808377 | 3.19E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene80293 | 0.815476 | 9.07E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene80744 | 0.816267 | 6.04E-52 | -1.69E-46 |
| MS.gene057820 | MS.gene81504 | 0.806414 | 8.32E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene81505 | 0.80115 | 1.03E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene81506 | 0.803282 | 3.76E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene82244 | 0.801149 | 1.03E-48 | -1.69E-46 |
| MS.gene057820 | MS.gene87056 | 0.811398 | 7.14E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene87222 | 0.80203 | 6.81E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene87450 | 0.804292 | 2.32E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene90936 | 0.813124 | 3.00E-51 | -1.69E-46 |
| MS.gene057820 | MS.gene91226 | 0.8067 | 7.24E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene95804 | 0.801481 | 8.82E-49 | -1.69E-46 |
| MS.gene057820 | MS.gene98322 | 0.807987 | 3.86E-50 | -1.69E-46 |
| MS.gene057820 | MS.gene98678 | -0.805847 | 1.10E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057820.t1 | MTR_4g072350 | 87.850 | 321 | 19 | 1 | 1 | 301 | 1 | 321 | 0.0 | 575 |
| MS.gene057820.t1 | MTR_4g072360 | 83.438 | 320 | 33 | 1 | 1 | 300 | 1 | 320 | 0.0 | 544 |
| MS.gene057820.t1 | MTR_4g072320 | 80.312 | 320 | 37 | 3 | 1 | 300 | 1 | 314 | 0.0 | 515 |
| MS.gene057820.t1 | MTR_6g073110 | 57.862 | 318 | 114 | 1 | 1 | 298 | 1 | 318 | 1.45e-137 | 392 |
| MS.gene057820.t1 | MTR_0374s0050 | 48.607 | 323 | 135 | 3 | 1 | 300 | 1 | 315 | 4.15e-104 | 306 |
| MS.gene057820.t1 | MTR_3g083130 | 45.483 | 321 | 139 | 7 | 3 | 298 | 4 | 313 | 6.47e-90 | 270 |
| MS.gene057820.t1 | MTR_4g036845 | 43.354 | 316 | 156 | 5 | 5 | 300 | 2 | 314 | 4.87e-83 | 253 |
| MS.gene057820.t1 | MTR_8g070095 | 42.492 | 313 | 153 | 6 | 11 | 300 | 10 | 318 | 2.18e-80 | 246 |
| MS.gene057820.t1 | MTR_5g097900 | 44.099 | 322 | 145 | 8 | 1 | 298 | 1 | 311 | 1.48e-77 | 239 |
| MS.gene057820.t1 | MTR_5g097910 | 45.183 | 301 | 139 | 6 | 1 | 278 | 1 | 298 | 2.81e-77 | 238 |
| MS.gene057820.t1 | MTR_5g097940 | 45.183 | 301 | 139 | 6 | 1 | 278 | 1 | 298 | 2.81e-77 | 238 |
| MS.gene057820.t1 | MTR_8g070115 | 40.439 | 319 | 163 | 5 | 3 | 300 | 2 | 314 | 1.18e-74 | 231 |
| MS.gene057820.t1 | MTR_7g063580 | 42.628 | 312 | 149 | 7 | 11 | 300 | 50 | 353 | 1.71e-70 | 222 |
| MS.gene057820.t1 | MTR_6g072650 | 70.000 | 150 | 23 | 1 | 152 | 301 | 10 | 137 | 8.18e-70 | 213 |
| MS.gene057820.t1 | MTR_4g021350 | 40.063 | 317 | 149 | 9 | 11 | 300 | 10 | 312 | 2.74e-69 | 218 |
| MS.gene057820.t1 | MTR_4g021410 | 41.290 | 310 | 142 | 8 | 11 | 298 | 11 | 302 | 2.77e-64 | 204 |
| MS.gene057820.t1 | MTR_4g021450 | 39.752 | 322 | 154 | 8 | 1 | 300 | 1 | 304 | 5.51e-63 | 201 |
| MS.gene057820.t1 | MTR_4g021410 | 42.960 | 277 | 123 | 6 | 43 | 298 | 3 | 265 | 2.90e-60 | 193 |
| MS.gene057820.t1 | MTR_4g021350 | 39.655 | 290 | 135 | 8 | 11 | 274 | 10 | 285 | 1.33e-59 | 192 |
| MS.gene057820.t1 | MTR_7g070500 | 36.656 | 311 | 165 | 9 | 11 | 298 | 17 | 318 | 1.18e-57 | 188 |
| MS.gene057820.t1 | MTR_4g072060 | 36.054 | 294 | 156 | 7 | 9 | 274 | 3 | 292 | 1.17e-51 | 172 |
| MS.gene057820.t1 | MTR_7g070500 | 35.689 | 283 | 153 | 6 | 11 | 273 | 17 | 290 | 4.40e-51 | 171 |
| MS.gene057820.t1 | MTR_4g021410 | 46.535 | 202 | 94 | 4 | 98 | 298 | 38 | 226 | 5.58e-51 | 168 |
| MS.gene057820.t1 | MTR_4g021450 | 45.588 | 204 | 97 | 4 | 98 | 300 | 38 | 228 | 2.84e-50 | 166 |
| MS.gene057820.t1 | MTR_4g021450 | 47.059 | 187 | 93 | 3 | 115 | 300 | 4 | 185 | 3.22e-49 | 162 |
| MS.gene057820.t1 | MTR_6g072710 | 62.832 | 113 | 42 | 0 | 170 | 282 | 1 | 113 | 6.20e-48 | 156 |
| MS.gene057820.t1 | MTR_6g072705 | 75.472 | 53 | 12 | 1 | 80 | 132 | 34 | 85 | 1.88e-22 | 89.4 |
| MS.gene057820.t1 | MTR_3g092140 | 23.469 | 294 | 156 | 7 | 35 | 274 | 72 | 350 | 5.81e-12 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057820.t1 | AT1G59960 | 45.231 | 325 | 153 | 3 | 1 | 300 | 1 | 325 | 9.76e-104 | 306 |
| MS.gene057820.t1 | AT1G59950 | 45.246 | 305 | 146 | 3 | 17 | 300 | 15 | 319 | 6.80e-94 | 281 |
| MS.gene057820.t1 | AT2G37790 | 45.513 | 312 | 140 | 6 | 11 | 300 | 10 | 313 | 2.63e-86 | 261 |
| MS.gene057820.t1 | AT2G37770 | 44.375 | 320 | 149 | 6 | 3 | 300 | 2 | 314 | 4.24e-84 | 256 |
| MS.gene057820.t1 | AT3G53880 | 43.910 | 312 | 146 | 6 | 11 | 300 | 10 | 314 | 1.99e-79 | 244 |
| MS.gene057820.t1 | AT5G62420 | 42.444 | 311 | 155 | 6 | 11 | 300 | 8 | 315 | 3.32e-79 | 243 |
| MS.gene057820.t1 | AT2G37760 | 41.479 | 311 | 151 | 5 | 11 | 300 | 10 | 310 | 2.74e-75 | 233 |
| MS.gene057820.t1 | AT2G37760 | 41.479 | 311 | 151 | 5 | 11 | 300 | 10 | 310 | 2.74e-75 | 233 |
| MS.gene057820.t1 | AT2G37760 | 41.319 | 288 | 139 | 4 | 11 | 278 | 10 | 287 | 5.90e-69 | 216 |
| MS.gene057820.t1 | AT2G37760 | 41.319 | 288 | 139 | 4 | 11 | 278 | 10 | 287 | 5.90e-69 | 216 |
| MS.gene057820.t1 | AT2G37760 | 41.608 | 286 | 137 | 4 | 11 | 276 | 10 | 285 | 9.78e-69 | 216 |
| MS.gene057820.t1 | AT3G53880 | 43.158 | 285 | 134 | 5 | 11 | 274 | 10 | 287 | 9.93e-69 | 216 |
| MS.gene057820.t1 | AT5G01670 | 38.782 | 312 | 157 | 9 | 11 | 298 | 18 | 319 | 2.40e-65 | 208 |
| MS.gene057820.t1 | AT5G01670 | 35.693 | 339 | 157 | 9 | 11 | 298 | 18 | 346 | 4.92e-61 | 197 |
| MS.gene057820.t1 | AT2G37760 | 41.923 | 260 | 127 | 4 | 62 | 300 | 8 | 264 | 2.38e-60 | 193 |
| MS.gene057820.t1 | AT2G37760 | 41.923 | 260 | 127 | 4 | 62 | 300 | 8 | 264 | 2.38e-60 | 193 |
| MS.gene057820.t1 | AT2G21260 | 35.932 | 295 | 157 | 6 | 8 | 274 | 2 | 292 | 3.28e-58 | 189 |
| MS.gene057820.t1 | AT2G21250 | 35.254 | 295 | 159 | 6 | 8 | 274 | 2 | 292 | 2.88e-57 | 187 |
| MS.gene057820.t1 | AT2G37770 | 45.238 | 210 | 87 | 5 | 3 | 191 | 2 | 204 | 8.27e-52 | 172 |
| MS.gene057820.t1 | AT2G21250 | 35.169 | 236 | 121 | 6 | 8 | 215 | 2 | 233 | 1.09e-40 | 142 |
| MS.gene057820.t1 | AT2G21260 | 35.176 | 199 | 119 | 3 | 86 | 274 | 26 | 224 | 1.42e-38 | 136 |
Find 76 sgRNAs with CRISPR-Local
Find 114 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGTCCTCACCTCAATTT+TGG | 0.184617 | 4.4:-36804183 | MS.gene057820:CDS |
| ATTAACAGCAGGAGAGATTT+TGG | 0.226195 | 4.4:+36803639 | None:intergenic |
| ACGTCTTGTTTCAACCTTAC+TGG | 0.266387 | 4.4:+36803814 | None:intergenic |
| TTTGGCACTTTCTAAGAGTA+TGG | 0.273981 | 4.4:+36803657 | None:intergenic |
| GTTGAAGGCCATAACTTTAA+AGG | 0.283083 | 4.4:-36803793 | MS.gene057820:CDS |
| TTGGTTGATGCCATTGAAAT+AGG | 0.299974 | 4.4:-36804164 | MS.gene057820:CDS |
| AATATGCCAATGATAGGTTT+TGG | 0.303449 | 4.4:-36804233 | MS.gene057820:CDS |
| TATCCTCACCTTTAAAGTTA+TGG | 0.331649 | 4.4:+36803785 | None:intergenic |
| TGACACCAATAGACTTTGAT+AGG | 0.343698 | 4.4:+36803704 | None:intergenic |
| TATGGAAGATTGTTATAGAT+TGG | 0.350814 | 4.4:-36803728 | MS.gene057820:CDS |
| CAATGGGAATTGCCCCTTGC+TGG | 0.357502 | 4.4:+36802840 | None:intergenic |
| AATCTCTCCTGCTGTTAATC+AGG | 0.358117 | 4.4:-36803635 | MS.gene057820:intron |
| AAAACATTGAAATATTTGAT+TGG | 0.366649 | 4.4:-36802787 | MS.gene057820:CDS |
| GAAATGTTTGTATCTGATAA+TGG | 0.381759 | 4.4:-36802701 | MS.gene057820:CDS |
| CCAGAGGTGATACTAAATTC+AGG | 0.400690 | 4.4:-36804260 | MS.gene057820:CDS |
| AAACATTGAAATATTTGATT+GGG | 0.403714 | 4.4:-36802786 | MS.gene057820:CDS |
| TGGATCTCTATTTGATTCAC+TGG | 0.404097 | 4.4:-36803837 | MS.gene057820:CDS |
| AGGAGGGTACAAACTAAGTT+GGG | 0.414798 | 4.4:-36803255 | MS.gene057820:CDS |
| GATGTTCCAAAACCTATCAT+TGG | 0.419966 | 4.4:+36804227 | None:intergenic |
| AGTTTCCCTTGTTGCCATGA+TGG | 0.420449 | 4.4:+36803329 | None:intergenic |
| ATGGAAGATTGTTATAGATT+GGG | 0.428752 | 4.4:-36803727 | MS.gene057820:CDS |
| CTATAATACTGAAGAACCTC+TGG | 0.433603 | 4.4:-36804114 | MS.gene057820:CDS |
| TCTATTGGTGTCAGTAACTT+TGG | 0.446190 | 4.4:-36803694 | MS.gene057820:CDS |
| ATTTGATTCACTGGCCAGTA+AGG | 0.449094 | 4.4:-36803828 | MS.gene057820:CDS |
| TAATGGACCTTACAAGTCAT+TGG | 0.455554 | 4.4:-36802684 | MS.gene057820:CDS |
| GTTTCCCTTGTTGCCATGAT+GGG | 0.465312 | 4.4:+36803330 | None:intergenic |
| AAATCTTGAATTGGAGTATG+TGG | 0.465434 | 4.4:-36803857 | MS.gene057820:CDS |
| CCTGAATTTAGTATCACCTC+TGG | 0.467537 | 4.4:+36804260 | None:intergenic |
| TCCTCAATGCAGACTGCTAA+AGG | 0.470577 | 4.4:-36802726 | MS.gene057820:CDS |
| TTGTTGAAGCTCTTCACAAT+GGG | 0.474196 | 4.4:+36802824 | None:intergenic |
| TAGAAAGAAGAGTGTAGCCC+AGG | 0.476626 | 4.4:-36803183 | MS.gene057820:intron |
| AAAAGGAACATGGGAAGCTA+TGG | 0.478713 | 4.4:-36803746 | MS.gene057820:CDS |
| AATGGCATCAACCAAAATTG+AGG | 0.495834 | 4.4:+36804172 | None:intergenic |
| TTGGGCCTATCAAAGTCTAT+TGG | 0.496646 | 4.4:-36803709 | MS.gene057820:CDS |
| TTGGGGTTCGCCTGCAGTGA+TGG | 0.500062 | 4.4:-36803237 | MS.gene057820:CDS |
| TAGGAGGGTACAAACTAAGT+TGG | 0.507601 | 4.4:-36803256 | MS.gene057820:CDS |
| ATCGAATTCTCCATCACTGC+AGG | 0.507726 | 4.4:+36803227 | None:intergenic |
| CAGAGGTGATACTAAATTCA+GGG | 0.521393 | 4.4:-36804259 | MS.gene057820:CDS |
| TTATACAGATAGCACTAAGA+TGG | 0.527906 | 4.4:-36802868 | MS.gene057820:intron |
| GTGTCAAAAGCTTTAGAGCT+AGG | 0.527948 | 4.4:-36804083 | MS.gene057820:CDS |
| CAAGTCATTGGAAGAGCTGT+GGG | 0.528282 | 4.4:-36802672 | MS.gene057820:CDS |
| AGCTCTTCCAATGACTTGTA+AGG | 0.535075 | 4.4:+36802677 | None:intergenic |
| GCCTTTAGCAGTCTGCATTG+AGG | 0.539010 | 4.4:+36802725 | None:intergenic |
| ATGCCTGTAGCCTATTTCAA+TGG | 0.544065 | 4.4:+36804154 | None:intergenic |
| ATGCCATTGAAATAGGCTAC+AGG | 0.548301 | 4.4:-36804157 | MS.gene057820:CDS |
| CTTGTTGAAGCTCTTCACAA+TGG | 0.556325 | 4.4:+36802823 | None:intergenic |
| AGGACTTCATGTGGTGGAGA+GGG | 0.561027 | 4.4:+36804197 | None:intergenic |
| AGGTGGAAATGAACCCATCA+TGG | 0.568456 | 4.4:-36803343 | MS.gene057820:CDS |
| TCATATACCTGATTAACAGC+AGG | 0.569876 | 4.4:+36803628 | None:intergenic |
| AGAGAGTTCTGCAAGCAGAA+AGG | 0.571100 | 4.4:-36803305 | MS.gene057820:CDS |
| GTGAGTGCATGGTCACCACT+AGG | 0.581102 | 4.4:-36803275 | MS.gene057820:CDS |
| ATGAACCCATCATGGCAACA+AGG | 0.585669 | 4.4:-36803335 | MS.gene057820:CDS |
| AAGGAATCCATGTGAGTGCA+TGG | 0.586898 | 4.4:-36803286 | MS.gene057820:CDS |
| ACAAGTCATTGGAAGAGCTG+TGG | 0.592554 | 4.4:-36802673 | MS.gene057820:CDS |
| TAAGATGGATACACCAGCAA+GGG | 0.593058 | 4.4:-36802853 | MS.gene057820:CDS |
| TGAACCCATCATGGCAACAA+GGG | 0.594189 | 4.4:-36803334 | MS.gene057820:CDS |
| GAGGACTTCATGTGGTGGAG+AGG | 0.594677 | 4.4:+36804196 | None:intergenic |
| AGGCCATAACTTTAAAGGTG+AGG | 0.603244 | 4.4:-36803788 | MS.gene057820:CDS |
| CATTGTGAAGAGCTTCAACA+AGG | 0.610965 | 4.4:-36802822 | MS.gene057820:CDS |
| GTGGTGACCATGCACTCACA+TGG | 0.614668 | 4.4:+36803279 | None:intergenic |
| CTTAGTTTGTACCCTCCTAG+TGG | 0.627177 | 4.4:+36803260 | None:intergenic |
| AGGTTGAAACAAGACGTTGA+AGG | 0.628014 | 4.4:-36803808 | MS.gene057820:CDS |
| TGAAGAGCTTCAACAAGGAG+AGG | 0.630692 | 4.4:-36802817 | MS.gene057820:CDS |
| TCATTGGAAGAGCTGTGGGA+TGG | 0.633458 | 4.4:-36802668 | MS.gene057820:CDS |
| TTTGACACAGCTTGGCCCAG+AGG | 0.635347 | 4.4:+36804098 | None:intergenic |
| TATAATACTGAAGAACCTCT+GGG | 0.636374 | 4.4:-36804113 | MS.gene057820:CDS |
| GATTCTGCGCAAAATTGCTG+AGG | 0.639021 | 4.4:-36803207 | MS.gene057820:CDS |
| AGTGCATGGTCACCACTAGG+AGG | 0.651978 | 4.4:-36803272 | MS.gene057820:CDS |
| GGAAACAAACAAAGTTCCAG+AGG | 0.669203 | 4.4:-36804276 | MS.gene057820:CDS |
| AAGATGGATACACCAGCAAG+GGG | 0.678333 | 4.4:-36802852 | MS.gene057820:CDS |
| GGAGGGTACAAACTAAGTTG+GGG | 0.682661 | 4.4:-36803254 | MS.gene057820:CDS |
| CATCAACCAAAATTGAGGTG+AGG | 0.683706 | 4.4:+36804177 | None:intergenic |
| GTGCATGGTCACCACTAGGA+GGG | 0.685225 | 4.4:-36803271 | MS.gene057820:CDS |
| CTAAGATGGATACACCAGCA+AGG | 0.696550 | 4.4:-36802854 | MS.gene057820:CDS |
| GAGGTGAGGACTTCATGTGG+TGG | 0.731533 | 4.4:+36804191 | None:intergenic |
| ATTGAGGTGAGGACTTCATG+TGG | 0.753402 | 4.4:+36804188 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACTTGAATTATAAAAT+AGG | + | chr4.4:36803873-36803892 | None:intergenic | 10.0% |
| !! | AAAACATTGAAATATTTGAT+TGG | - | chr4.4:36804149-36804168 | MS.gene057820:CDS | 15.0% |
| !! | AAACATTGAAATATTTGATT+GGG | - | chr4.4:36804150-36804169 | MS.gene057820:CDS | 15.0% |
| !! | AACTTCATTAAAATCTTAAT+TGG | + | chr4.4:36803836-36803855 | None:intergenic | 15.0% |
| !!! | ATTTTTTAGAAATCTTGAAT+TGG | - | chr4.4:36803070-36803089 | MS.gene057820:intron | 15.0% |
| !!! | TATTATTGTTTTGGTTTTTT+TGG | - | chr4.4:36803041-36803060 | MS.gene057820:intron | 15.0% |
| !! | ACTTAAAAGAAGTAGAAAAA+AGG | + | chr4.4:36802997-36803016 | None:intergenic | 20.0% |
| !!! | ACAAATTCTTATTTGGATTT+AGG | - | chr4.4:36803573-36803592 | MS.gene057820:intron | 20.0% |
| !!! | ACAATTCCTTTTGATATAAA+AGG | - | chr4.4:36803173-36803192 | MS.gene057820:intron | 20.0% |
| !!! | CTTGTCTAATATTATTGTTT+TGG | - | chr4.4:36803032-36803051 | MS.gene057820:intron | 20.0% |
| !!! | TAATTCAAGTTTTTTTTCCA+TGG | - | chr4.4:36803879-36803898 | MS.gene057820:intron | 20.0% |
| !!! | TAATTTTGTCTAATTCTTCT+TGG | + | chr4.4:36804183-36804202 | None:intergenic | 20.0% |
| !!! | TATTGGATACTATAAGTTTT+GGG | + | chr4.4:36803417-36803436 | None:intergenic | 20.0% |
| !!! | TTATTGGATACTATAAGTTT+TGG | + | chr4.4:36803418-36803437 | None:intergenic | 20.0% |
| !!! | TTTTGATATAAAAGGAACAT+GGG | - | chr4.4:36803181-36803200 | MS.gene057820:intron | 20.0% |
| ! | AATCTTAATTGGCTAAACAT+AGG | + | chr4.4:36803825-36803844 | None:intergenic | 25.0% |
| ! | ATGGAAGATTGTTATAGATT+GGG | - | chr4.4:36803209-36803228 | MS.gene057820:CDS | 25.0% |
| ! | GAAATGTTTGTATCTGATAA+TGG | - | chr4.4:36804235-36804254 | MS.gene057820:CDS | 25.0% |
| ! | GCATCATACAAATTCTTATT+TGG | - | chr4.4:36803566-36803585 | MS.gene057820:intron | 25.0% |
| ! | TATGGAAGATTGTTATAGAT+TGG | - | chr4.4:36803208-36803227 | MS.gene057820:CDS | 25.0% |
| !! | AATTCTTATTTGGATTTAGG+TGG | - | chr4.4:36803576-36803595 | MS.gene057820:intron | 25.0% |
| !! | CATGTTCCTTTTATATCAAA+AGG | + | chr4.4:36803182-36803201 | None:intergenic | 25.0% |
| !! | CTTTTGATATAAAAGGAACA+TGG | - | chr4.4:36803180-36803199 | MS.gene057820:intron | 25.0% |
| AAATCTTGAATTGGAGTATG+TGG | - | chr4.4:36803079-36803098 | MS.gene057820:intron | 30.0% | |
| ATAAAATAATCAACTGCACC+TGG | + | chr4.4:36803774-36803793 | None:intergenic | 30.0% | |
| TAAAATAATCAACTGCACCT+GGG | + | chr4.4:36803773-36803792 | None:intergenic | 30.0% | |
| TAACAGTAATCTCAATACAG+TGG | + | chr4.4:36804016-36804035 | None:intergenic | 30.0% | |
| TATAATACTGAAGAACCTCT+GGG | - | chr4.4:36802823-36802842 | MS.gene057820:CDS | 30.0% | |
| TGTTCATTACTTCCAAGTTA+TGG | - | chr4.4:36802899-36802918 | MS.gene057820:intron | 30.0% | |
| TTATACAGATAGCACTAAGA+TGG | - | chr4.4:36804068-36804087 | MS.gene057820:CDS | 30.0% | |
| TTGTTTCGCATAACAATCTA+AGG | + | chr4.4:36803951-36803970 | None:intergenic | 30.0% | |
| ! | AATATGCCAATGATAGGTTT+TGG | - | chr4.4:36802703-36802722 | MS.gene057820:CDS | 30.0% |
| ! | ATGTATTTGTGAAGTATCAC+TGG | + | chr4.4:36803469-36803488 | None:intergenic | 30.0% |
| !! | TATCCTCACCTTTAAAGTTA+TGG | + | chr4.4:36803154-36803173 | None:intergenic | 30.0% |
| AAATTATGCTTGCTTGAGAG+TGG | + | chr4.4:36802968-36802987 | None:intergenic | 35.0% | |
| AATGGCATCAACCAAAATTG+AGG | + | chr4.4:36802767-36802786 | None:intergenic | 35.0% | |
| CAGAGGTGATACTAAATTCA+GGG | - | chr4.4:36802677-36802696 | MS.gene057820:CDS | 35.0% | |
| CTATAATACTGAAGAACCTC+TGG | - | chr4.4:36802822-36802841 | MS.gene057820:CDS | 35.0% | |
| CTGCATCAGTTACTTCAAAA+TGG | - | chr4.4:36803358-36803377 | MS.gene057820:intron | 35.0% | |
| GATGTTCCAAAACCTATCAT+TGG | + | chr4.4:36802712-36802731 | None:intergenic | 35.0% | |
| GGGAAAAATATGCCAATGAT+AGG | - | chr4.4:36802697-36802716 | MS.gene057820:CDS | 35.0% | |
| GTTGAAGGCCATAACTTTAA+AGG | - | chr4.4:36803143-36803162 | MS.gene057820:intron | 35.0% | |
| TCATATACCTGATTAACAGC+AGG | + | chr4.4:36803311-36803330 | None:intergenic | 35.0% | |
| TGACACCAATAGACTTTGAT+AGG | + | chr4.4:36803235-36803254 | None:intergenic | 35.0% | |
| TGGATCTCTATTTGATTCAC+TGG | - | chr4.4:36803099-36803118 | MS.gene057820:intron | 35.0% | |
| TTGTTGAAGCTCTTCACAAT+GGG | + | chr4.4:36804115-36804134 | None:intergenic | 35.0% | |
| ! | ATTAACAGCAGGAGAGATTT+TGG | + | chr4.4:36803300-36803319 | None:intergenic | 35.0% |
| ! | GTTCATCACGATTTTTAACG+AGG | + | chr4.4:36802879-36802898 | None:intergenic | 35.0% |
| ! | TAATGGACCTTACAAGTCAT+TGG | - | chr4.4:36804252-36804271 | MS.gene057820:CDS | 35.0% |
| ! | TTGGTTGATGCCATTGAAAT+AGG | - | chr4.4:36802772-36802791 | MS.gene057820:CDS | 35.0% |
| ! | TTTGGCACTTTCTAAGAGTA+TGG | + | chr4.4:36803282-36803301 | None:intergenic | 35.0% |
| !! | TCTATTGGTGTCAGTAACTT+TGG | - | chr4.4:36803242-36803261 | MS.gene057820:CDS | 35.0% |
| AAAAGGAACATGGGAAGCTA+TGG | - | chr4.4:36803190-36803209 | MS.gene057820:CDS | 40.0% | |
| AATCTCTCCTGCTGTTAATC+AGG | - | chr4.4:36803301-36803320 | MS.gene057820:CDS | 40.0% | |
| AGCATCAGTACACCATAACT+TGG | + | chr4.4:36802914-36802933 | None:intergenic | 40.0% | |
| AGGTTGAAACAAGACGTTGA+AGG | - | chr4.4:36803128-36803147 | MS.gene057820:intron | 40.0% | |
| ATGCCATTGAAATAGGCTAC+AGG | - | chr4.4:36802779-36802798 | MS.gene057820:CDS | 40.0% | |
| ATGCCTGTAGCCTATTTCAA+TGG | + | chr4.4:36802785-36802804 | None:intergenic | 40.0% | |
| ATTTGATTCACTGGCCAGTA+AGG | - | chr4.4:36803108-36803127 | MS.gene057820:intron | 40.0% | |
| CATCAACCAAAATTGAGGTG+AGG | + | chr4.4:36802762-36802781 | None:intergenic | 40.0% | |
| CATTGTGAAGAGCTTCAACA+AGG | - | chr4.4:36804114-36804133 | MS.gene057820:CDS | 40.0% | |
| CCAGAGGTGATACTAAATTC+AGG | - | chr4.4:36802676-36802695 | MS.gene057820:CDS | 40.0% | |
| CCATGGTCAAACTTTAGACT+TGG | - | chr4.4:36803896-36803915 | MS.gene057820:intron | 40.0% | |
| CCTGAATTTAGTATCACCTC+TGG | + | chr4.4:36802679-36802698 | None:intergenic | 40.0% | |
| CTTGTTGAAGCTCTTCACAA+TGG | + | chr4.4:36804116-36804135 | None:intergenic | 40.0% | |
| GGAAACAAACAAAGTTCCAG+AGG | - | chr4.4:36802660-36802679 | MS.gene057820:CDS | 40.0% | |
| TAAGATGGATACACCAGCAA+GGG | - | chr4.4:36804083-36804102 | MS.gene057820:CDS | 40.0% | |
| TTGGGCCTATCAAAGTCTAT+TGG | - | chr4.4:36803227-36803246 | MS.gene057820:CDS | 40.0% | |
| ! | AGCTCTTCCAATGACTTGTA+AGG | + | chr4.4:36804262-36804281 | None:intergenic | 40.0% |
| ! | AGGAGGGTACAAACTAAGTT+GGG | - | chr4.4:36803681-36803700 | MS.gene057820:CDS | 40.0% |
| ! | AGGCCATAACTTTAAAGGTG+AGG | - | chr4.4:36803148-36803167 | MS.gene057820:intron | 40.0% |
| ! | CCAAGTCTAAAGTTTGACCA+TGG | + | chr4.4:36803899-36803918 | None:intergenic | 40.0% |
| ! | CTAAAGCTTTTGACACAGCT+TGG | + | chr4.4:36802849-36802868 | None:intergenic | 40.0% |
| ! | TAGGAGGGTACAAACTAAGT+TGG | - | chr4.4:36803680-36803699 | MS.gene057820:CDS | 40.0% |
| ! | TGAAGTCCTCACCTCAATTT+TGG | - | chr4.4:36802753-36802772 | MS.gene057820:CDS | 40.0% |
| !! | ACGTCTTGTTTCAACCTTAC+TGG | + | chr4.4:36803125-36803144 | None:intergenic | 40.0% |
| !! | GTGTCAAAAGCTTTAGAGCT+AGG | - | chr4.4:36802853-36802872 | MS.gene057820:CDS | 40.0% |
| AAGATGGATACACCAGCAAG+GGG | - | chr4.4:36804084-36804103 | MS.gene057820:CDS | 45.0% | |
| AAGGAATCCATGTGAGTGCA+TGG | - | chr4.4:36803650-36803669 | MS.gene057820:CDS | 45.0% | |
| ACAAGTCATTGGAAGAGCTG+TGG | - | chr4.4:36804263-36804282 | MS.gene057820:CDS | 45.0% | |
| AGAGAGTTCTGCAAGCAGAA+AGG | - | chr4.4:36803631-36803650 | MS.gene057820:intron | 45.0% | |
| AGGTGGAAATGAACCCATCA+TGG | - | chr4.4:36803593-36803612 | MS.gene057820:intron | 45.0% | |
| AGTTTCCCTTGTTGCCATGA+TGG | + | chr4.4:36803610-36803629 | None:intergenic | 45.0% | |
| ATCGAATTCTCCATCACTGC+AGG | + | chr4.4:36803712-36803731 | None:intergenic | 45.0% | |
| ATGAACCCATCATGGCAACA+AGG | - | chr4.4:36803601-36803620 | MS.gene057820:intron | 45.0% | |
| ATGACAACTGCGTGCTTAGT+TGG | + | chr4.4:36803508-36803527 | None:intergenic | 45.0% | |
| ATTGAGGTGAGGACTTCATG+TGG | + | chr4.4:36802751-36802770 | None:intergenic | 45.0% | |
| CAAGTCATTGGAAGAGCTGT+GGG | - | chr4.4:36804264-36804283 | MS.gene057820:CDS | 45.0% | |
| CTAAGATGGATACACCAGCA+AGG | - | chr4.4:36804082-36804101 | MS.gene057820:CDS | 45.0% | |
| GATTCTGCGCAAAATTGCTG+AGG | - | chr4.4:36803729-36803748 | MS.gene057820:CDS | 45.0% | |
| GTTTCCCTTGTTGCCATGAT+GGG | + | chr4.4:36803609-36803628 | None:intergenic | 45.0% | |
| TCCTCAATGCAGACTGCTAA+AGG | - | chr4.4:36804210-36804229 | MS.gene057820:CDS | 45.0% | |
| TGAACCCATCATGGCAACAA+GGG | - | chr4.4:36803602-36803621 | MS.gene057820:intron | 45.0% | |
| TGAAGAGCTTCAACAAGGAG+AGG | - | chr4.4:36804119-36804138 | MS.gene057820:CDS | 45.0% | |
| ! | GGAGGGTACAAACTAAGTTG+GGG | - | chr4.4:36803682-36803701 | MS.gene057820:CDS | 45.0% |
| ! | TAGAAAGAAGAGTGTAGCCC+AGG | - | chr4.4:36803753-36803772 | MS.gene057820:CDS | 45.0% |
| !! | CTTAGTTTGTACCCTCCTAG+TGG | + | chr4.4:36803679-36803698 | None:intergenic | 45.0% |
| !! | TTTTGAGAGCTGGGAGAACA+AGG | + | chr4.4:36802945-36802964 | None:intergenic | 45.0% |
| !!! | TGAGAGTGGTTTTGAGAGCT+GGG | + | chr4.4:36802954-36802973 | None:intergenic | 45.0% |
| !!! | TTGAGAGTGGTTTTGAGAGC+TGG | + | chr4.4:36802955-36802974 | None:intergenic | 45.0% |
| !! | AAAGAATTAAAATAAATTAT+TGG | + | chr4.4:36803434-36803453 | None:intergenic | 5.0% |
| !!! | ATAATTTATTTTAATTCTTT+TGG | - | chr4.4:36803434-36803453 | MS.gene057820:intron | 5.0% |
| GCCTTTAGCAGTCTGCATTG+AGG | + | chr4.4:36804214-36804233 | None:intergenic | 50.0% | |
| TCATTGGAAGAGCTGTGGGA+TGG | - | chr4.4:36804268-36804287 | MS.gene057820:CDS | 50.0% | |
| ! | AGGACTTCATGTGGTGGAGA+GGG | + | chr4.4:36802742-36802761 | None:intergenic | 50.0% |
| AGTGCATGGTCACCACTAGG+AGG | - | chr4.4:36803664-36803683 | MS.gene057820:CDS | 55.0% | |
| CAATGGGAATTGCCCCTTGC+TGG | + | chr4.4:36804099-36804118 | None:intergenic | 55.0% | |
| GAGGTGAGGACTTCATGTGG+TGG | + | chr4.4:36802748-36802767 | None:intergenic | 55.0% | |
| GTGAGTGCATGGTCACCACT+AGG | - | chr4.4:36803661-36803680 | MS.gene057820:CDS | 55.0% | |
| GTGCATGGTCACCACTAGGA+GGG | - | chr4.4:36803665-36803684 | MS.gene057820:CDS | 55.0% | |
| GTGGTGACCATGCACTCACA+TGG | + | chr4.4:36803660-36803679 | None:intergenic | 55.0% | |
| TTTGACACAGCTTGGCCCAG+AGG | + | chr4.4:36802841-36802860 | None:intergenic | 55.0% | |
| ! | GAGGACTTCATGTGGTGGAG+AGG | + | chr4.4:36802743-36802762 | None:intergenic | 55.0% |
| TTGGGGTTCGCCTGCAGTGA+TGG | - | chr4.4:36803699-36803718 | MS.gene057820:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 36802658 | 36804300 | 36802658 | ID=MS.gene057820 |
| chr4.4 | mRNA | 36802658 | 36804300 | 36802658 | ID=MS.gene057820.t1;Parent=MS.gene057820 |
| chr4.4 | exon | 36804044 | 36804300 | 36804044 | ID=MS.gene057820.t1.exon1;Parent=MS.gene057820.t1 |
| chr4.4 | CDS | 36804044 | 36804300 | 36804044 | ID=cds.MS.gene057820.t1;Parent=MS.gene057820.t1 |
| chr4.4 | exon | 36803636 | 36803879 | 36803636 | ID=MS.gene057820.t1.exon2;Parent=MS.gene057820.t1 |
| chr4.4 | CDS | 36803636 | 36803879 | 36803636 | ID=cds.MS.gene057820.t1;Parent=MS.gene057820.t1 |
| chr4.4 | exon | 36803184 | 36803363 | 36803184 | ID=MS.gene057820.t1.exon3;Parent=MS.gene057820.t1 |
| chr4.4 | CDS | 36803184 | 36803363 | 36803184 | ID=cds.MS.gene057820.t1;Parent=MS.gene057820.t1 |
| chr4.4 | exon | 36802658 | 36802882 | 36802658 | ID=MS.gene057820.t1.exon4;Parent=MS.gene057820.t1 |
| chr4.4 | CDS | 36802658 | 36802882 | 36802658 | ID=cds.MS.gene057820.t1;Parent=MS.gene057820.t1 |
| Gene Sequence |
| Protein sequence |
