AlfalfaGEDB | MS.gene85003

M. sativa cultivar XinJiangDaYe / MS.gene85003

KEGG: K10899
GO: -
PFAM: PF16888.8

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85003.t1	RHN54641.1	93.1	87	6	0	1	87	1	87	4.70E-20	107.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85003.t1	Q9FT73	51.1	45	22	0	43	87	43	87	6.6e-07	54.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85003.t1	G7K6R5	93.1	87	6	0	1	87	1	87	3.4e-20	107.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049159	MS.gene85003	0.815097	1.10E-51	-1.69E-46
MS.gene049501	MS.gene85003	0.805593	1.24E-49	-1.69E-46
MS.gene050020	MS.gene85003	0.814285	1.67E-51	-1.69E-46
MS.gene050438	MS.gene85003	0.810347	1.21E-50	-1.69E-46
MS.gene050442	MS.gene85003	0.814228	1.71E-51	-1.69E-46
MS.gene050586	MS.gene85003	0.815027	1.14E-51	-1.69E-46
MS.gene050807	MS.gene85003	0.829874	4.03E-55	-1.69E-46
MS.gene050815	MS.gene85003	0.815656	8.27E-52	-1.69E-46
MS.gene050959	MS.gene85003	0.824224	9.06E-54	-1.69E-46
MS.gene051265	MS.gene85003	0.80981	1.57E-50	-1.69E-46
MS.gene051388	MS.gene85003	0.806182	9.31E-50	-1.69E-46
MS.gene05157	MS.gene85003	0.815256	1.01E-51	-1.69E-46
MS.gene051719	MS.gene85003	0.801818	7.53E-49	-1.69E-46
MS.gene052020	MS.gene85003	0.835824	1.34E-56	-1.69E-46
MS.gene052323	MS.gene85003	0.802519	5.40E-49	-1.69E-46
MS.gene052611	MS.gene85003	0.826087	3.29E-54	-1.69E-46
MS.gene052665	MS.gene85003	0.840649	7.66E-58	-1.69E-46
MS.gene052863	MS.gene85003	0.809281	2.04E-50	-1.69E-46
MS.gene052896	MS.gene85003	0.824025	1.01E-53	-1.69E-46
MS.gene053074	MS.gene85003	0.807806	4.22E-50	-1.69E-46
MS.gene053226	MS.gene85003	0.800591	1.34E-48	-1.69E-46
MS.gene054467	MS.gene85003	0.804429	2.17E-49	-1.69E-46
MS.gene055358	MS.gene85003	0.802322	5.93E-49	-1.69E-46
MS.gene055465	MS.gene85003	0.804928	1.71E-49	-1.69E-46
MS.gene055466	MS.gene85003	0.81531	9.87E-52	-1.69E-46
MS.gene055512	MS.gene85003	0.811924	5.49E-51	-1.69E-46
MS.gene055608	MS.gene85003	0.823764	1.16E-53	-1.69E-46
MS.gene056173	MS.gene85003	0.803795	2.94E-49	-1.69E-46
MS.gene056553	MS.gene85003	0.8375	5.01E-57	-1.69E-46
MS.gene057444	MS.gene85003	0.807488	4.93E-50	-1.69E-46
MS.gene057446	MS.gene85003	0.803966	2.71E-49	-1.69E-46
MS.gene057869	MS.gene85003	0.810778	9.73E-51	-1.69E-46
MS.gene057915	MS.gene85003	0.809518	1.82E-50	-1.69E-46
MS.gene058168	MS.gene85003	0.810116	1.35E-50	-1.69E-46
MS.gene059937	MS.gene85003	0.83258	8.72E-56	-1.69E-46
MS.gene060137	MS.gene85003	-0.817643	2.96E-52	-1.69E-46
MS.gene060322	MS.gene85003	0.802524	5.39E-49	-1.69E-46
MS.gene060470	MS.gene85003	0.801361	9.34E-49	-1.69E-46
MS.gene060480	MS.gene85003	0.809008	2.34E-50	-1.69E-46
MS.gene060803	MS.gene85003	0.817449	3.28E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85003.t1	MTR_5g026590	96.552	87	3	0	1	87	1	87	3.54e-52	173

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene85003.t1	AT1G31360	52.874	87	41	1	87	1	87	7.07e-27	102
MS.gene85003.t1	AT1G31360	52.874	87	41	1	87	1	87	7.77e-27	102
MS.gene85003.t1	AT1G31360	52.874	87	41	1	87	1	87	7.77e-27	102
MS.gene85003.t1	AT1G31360	52.874	87	41	1	87	35	121	8.57e-27	102
MS.gene85003.t1	AT1G31360	52.874	87	41	1	87	1	87	8.69e-27	102
MS.gene85003.t1	AT1G31360	52.874	87	41	1	87	1	87	9.87e-27	102
MS.gene85003.t1	AT1G31360	52.874	87	41	1	87	1	87	1.07e-26	102

Find 11 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATCTGAACTAAATTCGATTT+TGG	0.157671	5.2:+17885919	MS.gene85003:CDS
ATTCTCAAGCTGATGATCTT+AGG	0.305060	5.2:+17886026	MS.gene85003:CDS
GTTCGAGTGTTGTGGAGAAT+TGG	0.383508	5.2:+17885984	MS.gene85003:CDS
GTGTAAAGAATCTGAAAATC+AGG	0.408828	5.2:+17885946	MS.gene85003:CDS
ATTGGAACGGGACGTTTGAA+TGG	0.415913	5.2:+17886002	MS.gene85003:CDS
TTGGAACGGGACGTTTGAAT+GGG	0.503155	5.2:+17886003	MS.gene85003:CDS
GCTGAAATCGAAGATGTTCA+AGG	0.509003	5.2:+17885747	MS.gene85003:CDS
GTGTTGTGGAGAATTGGAAC+GGG	0.540609	5.2:+17885990	MS.gene85003:CDS
TGCTGCAAGTTCGAGTGTTG+TGG	0.580841	5.2:+17885976	MS.gene85003:CDS
AGTGTTGTGGAGAATTGGAA+CGG	0.602354	5.2:+17885989	MS.gene85003:CDS
CGTTAATTGAAAAGCAAGAG+AGG	0.608856	5.2:+17885882	MS.gene85003:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATGATTGTTATTATGATT+TGG	-	chr5.2:17885802-17885821	None:intergenic	15.0%
!!	TTAATCAGCTAATTTAGTTA+TGG	-	chr5.2:17885833-17885852	None:intergenic	20.0%
!!	ATCTGAACTAAATTCGATTT+TGG	+	chr5.2:17885919-17885938	MS.gene85003:CDS	25.0%
!!	CATTTTCTGCAGAAAAAATT+AGG	+	chr5.2:17885858-17885877	MS.gene85003:intron	25.0%
!!	TGATTTGGAAGAAAAATTGA+AGG	-	chr5.2:17885787-17885806	None:intergenic	25.0%
!!!	GATCTTAGGTTGAATATTTT+TGG	+	chr5.2:17886040-17886059	MS.gene85003:CDS	25.0%
	GTGTAAAGAATCTGAAAATC+AGG	+	chr5.2:17885946-17885965	MS.gene85003:CDS	30.0%
	ATTCTCAAGCTGATGATCTT+AGG	+	chr5.2:17886026-17886045	MS.gene85003:CDS	35.0%
	CGTTAATTGAAAAGCAAGAG+AGG	+	chr5.2:17885882-17885901	MS.gene85003:CDS	35.0%
	AGTGTTGTGGAGAATTGGAA+CGG	+	chr5.2:17885989-17886008	MS.gene85003:CDS	40.0%
	GCTGAAATCGAAGATGTTCA+AGG	+	chr5.2:17885747-17885766	MS.gene85003:CDS	40.0%
	ATTGGAACGGGACGTTTGAA+TGG	+	chr5.2:17886002-17886021	MS.gene85003:CDS	45.0%
	GTGTTGTGGAGAATTGGAAC+GGG	+	chr5.2:17885990-17886009	MS.gene85003:CDS	45.0%
	TTGGAACGGGACGTTTGAAT+GGG	+	chr5.2:17886003-17886022	MS.gene85003:CDS	45.0%
!	GTTCGAGTGTTGTGGAGAAT+TGG	+	chr5.2:17885984-17886003	MS.gene85003:CDS	45.0%
!	TGCTGCAAGTTCGAGTGTTG+TGG	+	chr5.2:17885976-17885995	MS.gene85003:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	17885705	17886092	17885705	ID=MS.gene85003
chr5.2	mRNA	17885705	17886092	17885705	ID=MS.gene85003.t1;Parent=MS.gene85003
chr5.2	exon	17885705	17885768	17885705	ID=MS.gene85003.t1.exon1;Parent=MS.gene85003.t1
chr5.2	CDS	17885705	17885768	17885705	ID=cds.MS.gene85003.t1;Parent=MS.gene85003.t1
chr5.2	exon	17885870	17886092	17885870	ID=MS.gene85003.t1.exon2;Parent=MS.gene85003.t1
chr5.2	CDS	17885870	17886092	17885870	ID=cds.MS.gene85003.t1;Parent=MS.gene85003.t1

Gene Sequence

>MS.gene85003

ATGGAAAACTACGAAGTTCTTGAAGAGCTTCACAACGTCGAAGCTGAAATCGAAGATGTTCAAGGTGCTTTTTTTTTTTCCTTCAATTTTTCTTCCAAATCATAATAACAATCATAATGTTAATGCCATAACTAAATTAGCTGATTAATTTTTCATTTTCTGCAGAAAAAATTAGGTCGTTAATTGAAAAGCAAGAGAGGTTGTACGAGAGAAAATCTGAACTAAATTCGATTTTGGAAGCGTGTAAAGAATCTGAAAATCAGGTTAATAATGCTGCAAGTTCGAGTGTTGTGGAGAATTGGAACGGGACGTTTGAATGGGATTCTCAAGCTGATGATCTTAGGTTGAATATTTTTGGTATTCATCTTATCGTGCTAATCAAAAAGAG

Protein sequence

>MS.gene85003.t1

MENYEVLEELHNVEAEIEDVQEKIRSLIEKQERLYERKSELNSILEACKESENQVNNAASSSVVENWNGTFEWDSQADDLRLNIFGIHLIVLIKK