AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21474


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21474.t1 MTR_5g014260 100.000 166 0 0 1 166 1 166 6.40e-121 338
MS.gene21474.t1 MTR_5g014260 99.200 125 1 0 1 125 1 125 2.21e-87 252
MS.gene21474.t1 MTR_4g084110 59.880 167 54 3 1 166 1 155 2.45e-69 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21474.t1 AT1G32470 82.530 166 29 0 1 166 1 166 1.20e-93 269
MS.gene21474.t1 AT2G35370 83.735 166 26 1 1 166 1 165 9.20e-90 259
MS.gene21474.t1 AT2G35120 58.929 168 55 3 1 166 1 156 1.44e-64 195

Find 46 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACTGAACCGCCAGGTTC+TGG 0.305688 5.3:+7449675 None:intergenic
ATCATCCATCCATCTTCATA+TGG 0.339685 5.3:+7449188 None:intergenic
GTTACAAAGGGTAACGGTTT+CGG 0.348504 5.3:-7449657 MS.gene21474:CDS
AAACATCTGGAGATGGAAAA+AGG 0.352185 5.3:+7450320 None:intergenic
TCAACAATCTCACCAGATAT+TGG 0.373303 5.3:+7449594 None:intergenic
ATCTCCAGATGTTTCTCAAC+TGG 0.379569 5.3:-7450311 MS.gene21474:intron
TCTTGGAGAGGTTGTGTTTG+TGG 0.407524 5.3:-7449703 MS.gene21474:CDS
CGGAGCTGTTGAAAGTGTTA+AGG 0.409242 5.3:-7449637 MS.gene21474:CDS
GATGTAAACTCTCCAATATC+TGG 0.410884 5.3:-7449606 MS.gene21474:CDS
AGTATGCATCTTCACATGAA+TGG 0.426720 5.3:-7450187 MS.gene21474:CDS
TAACAATGGCACTGAGGATG+TGG 0.446358 5.3:-7450406 None:intergenic
AGAAGAGAGTTTGAGAGCAT+TGG 0.453540 5.3:+7450370 None:intergenic
ATCTGAATTAGAATCCTTGT+TGG 0.458285 5.3:-7449144 MS.gene21474:CDS
GATAAGCTCACCGGAAAACC+TGG 0.464278 5.3:-7449564 MS.gene21474:CDS
TGTTGGTTGCAGGACCATCT+TGG 0.467787 5.3:-7449720 MS.gene21474:intron
GAAGGCTCAGTAGCCACTAT+TGG 0.468590 5.3:-7450155 MS.gene21474:CDS
TCTAATTCAGATGGATCACT+TGG 0.485314 5.3:+7449155 None:intergenic
TCATCCATCCATCTTCATAT+GGG 0.487407 5.3:+7449189 None:intergenic
GTATGCATCTTCACATGAAT+GGG 0.495142 5.3:-7450186 MS.gene21474:CDS
GATACCAGTTGAGAAACATC+TGG 0.497324 5.3:+7450307 None:intergenic
AGAATCCTTGTTGGGTGCAA+AGG 0.506140 5.3:-7449135 MS.gene21474:CDS
TCTGAATTAGAATCCTTGTT+GGG 0.506709 5.3:-7449143 MS.gene21474:CDS
AATGAATGAATTACCAGGCC+AGG 0.510526 5.3:+7449546 None:intergenic
TTTGTGGAGCTTCCAGAACC+TGG 0.512073 5.3:-7449687 MS.gene21474:CDS
TGGTATCACTGACCATGCTC+AGG 0.528384 5.3:-7450135 MS.gene21474:intron
GCTCACCGGAAAACCTGGCC+TGG 0.529026 5.3:-7449559 MS.gene21474:intron
AACTCAAGCCCATATGAAGA+TGG 0.530377 5.3:-7449197 MS.gene21474:CDS
AACAAGGATTCTAATTCAGA+TGG 0.546174 5.3:+7449146 None:intergenic
AGGGGAGAGATGTAATCTTG+AGG 0.555174 5.3:+7450343 None:intergenic
CATGAATGGGTGAAGCATGA+AGG 0.562003 5.3:-7450173 MS.gene21474:CDS
CCCTTTGTAACTGAACCGCC+AGG 0.564034 5.3:+7449669 None:intergenic
CAAGCCCATATGAAGATGGA+TGG 0.567565 5.3:-7449193 MS.gene21474:CDS
ACCTGGCGGTTCAGTTACAA+AGG 0.580268 5.3:-7449670 MS.gene21474:CDS
AGTTGAGAAACATCTGGAGA+TGG 0.581853 5.3:+7450313 None:intergenic
GTTGCAGGACCATCTTGGAG+AGG 0.582318 5.3:-7449715 MS.gene21474:intron
GGTTCAGTTACAAAGGGTAA+CGG 0.588941 5.3:-7449663 MS.gene21474:CDS
CAAACACAACCTCTCCAAGA+TGG 0.593260 5.3:+7449706 None:intergenic
GAGGTTAATGATAAGCTCAC+CGG 0.595144 5.3:-7449573 MS.gene21474:CDS
AATATCTGGTGAGATTGTTG+AGG 0.595757 5.3:-7449592 MS.gene21474:CDS
AACAATGGCACTGAGGATGT+GGG 0.614850 5.3:-7450405 None:intergenic
CCTGGCGGTTCAGTTACAAA+GGG 0.619186 5.3:-7449669 MS.gene21474:CDS
ATGGTCAGTGATACCAATAG+TGG 0.628719 5.3:+7450142 None:intergenic
GTACTCCTTTGCACCCAACA+AGG 0.640084 5.3:+7449130 None:intergenic
GCAGTTAACTAACCTGAGCA+TGG 0.648601 5.3:+7450123 None:intergenic
GTGGAGCTTCCAGAACCTGG+CGG 0.691995 5.3:-7449684 MS.gene21474:CDS
TAGTCTTAACAATGGCACTG+AGG 0.723094 5.3:-7450412 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTACATTTATGAAAAATAAG+CGG - chr5.3:7450116-7450135 MS.gene21474:intron 20.0%
!! TGAATTAGACTTTAATTACT+AGG - chr5.3:7449586-7449605 MS.gene21474:CDS 20.0%
!!! TAAATAAATTTTTGCAGTGT+TGG - chr5.3:7449276-7449295 MS.gene21474:intron 20.0%
! AATCATTACAACTTAAGTGA+AGG - chr5.3:7449727-7449746 MS.gene21474:intron 25.0%
! GTTTAATTAATTGCACTTGT+TGG - chr5.3:7449758-7449777 MS.gene21474:intron 25.0%
!! GCTAGTATTTTATCTTTTGT+CGG + chr5.3:7449686-7449705 None:intergenic 25.0%
!!! ATACGAATTAGTCTGTTTTT+GGG - chr5.3:7449484-7449503 MS.gene21474:intron 25.0%
!!! ATTAACTCACTCTCATTTTT+AGG - chr5.3:7449969-7449988 MS.gene21474:intron 25.0%
!!! TATACGAATTAGTCTGTTTT+TGG - chr5.3:7449483-7449502 MS.gene21474:intron 25.0%
AACAAGGATTCTAATTCAGA+TGG + chr5.3:7450352-7450371 None:intergenic 30.0%
ACATATTGTGGAGAATGTTA+AGG + chr5.3:7449407-7449426 None:intergenic 30.0%
ATCTGAATTAGAATCCTTGT+TGG - chr5.3:7450351-7450370 MS.gene21474:CDS 30.0%
ATTGAGCTAAGAGTTAAATG+AGG - chr5.3:7449639-7449658 MS.gene21474:CDS 30.0%
CATTCAACTTGATAGGAAAA+AGG + chr5.3:7449230-7449249 None:intergenic 30.0%
GAGTTAATGAATGAATTACC+AGG + chr5.3:7449957-7449976 None:intergenic 30.0%
TCTGAATTAGAATCCTTGTT+GGG - chr5.3:7450352-7450371 MS.gene21474:CDS 30.0%
! AAAAGGAGGGTATATATGAA+AGG + chr5.3:7449213-7449232 None:intergenic 30.0%
!! TAAATTTTTGCAGTGTTGGA+TGG - chr5.3:7449280-7449299 MS.gene21474:intron 30.0%
AAACATCTGGAGATGGAAAA+AGG + chr5.3:7449178-7449197 None:intergenic 35.0%
AAGACATAAGTGAACTAGAG+GGG - chr5.3:7450094-7450113 MS.gene21474:intron 35.0%
AATATCTGGTGAGATTGTTG+AGG - chr5.3:7449903-7449922 MS.gene21474:intron 35.0%
AGTATGCATCTTCACATGAA+TGG - chr5.3:7449308-7449327 MS.gene21474:intron 35.0%
ATCATCCATCCATCTTCATA+TGG + chr5.3:7450310-7450329 None:intergenic 35.0%
CAACTTGATAGGAAAAAGGA+GGG + chr5.3:7449226-7449245 None:intergenic 35.0%
CCACATTCATTCAACTTGAT+AGG + chr5.3:7449237-7449256 None:intergenic 35.0%
GAAGACATAAGTGAACTAGA+GGG - chr5.3:7450093-7450112 MS.gene21474:intron 35.0%
GATGTAAACTCTCCAATATC+TGG - chr5.3:7449889-7449908 MS.gene21474:intron 35.0%
GTATGCATCTTCACATGAAT+GGG - chr5.3:7449309-7449328 MS.gene21474:intron 35.0%
TCAACAATCTCACCAGATAT+TGG + chr5.3:7449904-7449923 None:intergenic 35.0%
TCAACTTGATAGGAAAAAGG+AGG + chr5.3:7449227-7449246 None:intergenic 35.0%
TCATCCATCCATCTTCATAT+GGG + chr5.3:7450309-7450328 None:intergenic 35.0%
TCTAATTCAGATGGATCACT+TGG + chr5.3:7450343-7450362 None:intergenic 35.0%
TGAAGACATAAGTGAACTAG+AGG - chr5.3:7450092-7450111 MS.gene21474:intron 35.0%
! GGAGTACACAAAATTTTGTG+AGG - chr5.3:7450381-7450400 MS.gene21474:CDS 35.0%
!! CCTATCAAGTTGAATGAATG+TGG - chr5.3:7449234-7449253 MS.gene21474:intron 35.0%
!!! GAATTAGTCTGTTTTTGGGT+CGG - chr5.3:7449488-7449507 MS.gene21474:intron 35.0%
AACTCAAGCCCATATGAAGA+TGG - chr5.3:7450298-7450317 MS.gene21474:intron 40.0%
AATCTCACAGTTCACTTGTG+GGG + chr5.3:7449459-7449478 None:intergenic 40.0%
AATGAATGAATTACCAGGCC+AGG + chr5.3:7449952-7449971 None:intergenic 40.0%
AGTTGAGAAACATCTGGAGA+TGG + chr5.3:7449185-7449204 None:intergenic 40.0%
ATCTCCAGATGTTTCTCAAC+TGG - chr5.3:7449184-7449203 MS.gene21474:CDS 40.0%
ATCTGGAGATGGAAAAAGGA+GGG + chr5.3:7449174-7449193 None:intergenic 40.0%
ATGGTCAGTGATACCAATAG+TGG + chr5.3:7449356-7449375 None:intergenic 40.0%
CAATCTCACAGTTCACTTGT+GGG + chr5.3:7449460-7449479 None:intergenic 40.0%
GAGGTTAATGATAAGCTCAC+CGG - chr5.3:7449922-7449941 MS.gene21474:intron 40.0%
GATACCAGTTGAGAAACATC+TGG + chr5.3:7449191-7449210 None:intergenic 40.0%
GGTTCAGTTACAAAGGGTAA+CGG - chr5.3:7449832-7449851 MS.gene21474:intron 40.0%
TCAATCTCACAGTTCACTTG+TGG + chr5.3:7449461-7449480 None:intergenic 40.0%
TGGAGAATGTTAAGGAGTAG+AGG + chr5.3:7449399-7449418 None:intergenic 40.0%
! AGAAGAGAGTTTGAGAGCAT+TGG + chr5.3:7449128-7449147 None:intergenic 40.0%
! GTTACAAAGGGTAACGGTTT+CGG - chr5.3:7449838-7449857 MS.gene21474:intron 40.0%
! TAATTGCACTTGTTGGTTGC+AGG - chr5.3:7449765-7449784 MS.gene21474:intron 40.0%
AGAATCCTTGTTGGGTGCAA+AGG - chr5.3:7450360-7450379 MS.gene21474:CDS 45.0%
AGGGGAGAGATGTAATCTTG+AGG + chr5.3:7449155-7449174 None:intergenic 45.0%
ATCTCACAGTTCACTTGTGG+GGG + chr5.3:7449458-7449477 None:intergenic 45.0%
CAAACACAACCTCTCCAAGA+TGG + chr5.3:7449792-7449811 None:intergenic 45.0%
CAAGCCCATATGAAGATGGA+TGG - chr5.3:7450302-7450321 MS.gene21474:intron 45.0%
CATCTGGAGATGGAAAAAGG+AGG + chr5.3:7449175-7449194 None:intergenic 45.0%
CATGAATGGGTGAAGCATGA+AGG - chr5.3:7449322-7449341 MS.gene21474:intron 45.0%
CGCTTTGCATGGACATATTG+TGG + chr5.3:7449419-7449438 None:intergenic 45.0%
GCAGTTAACTAACCTGAGCA+TGG + chr5.3:7449375-7449394 None:intergenic 45.0%
TCTGGAGATGGAAAAAGGAG+GGG + chr5.3:7449173-7449192 None:intergenic 45.0%
TCTTGGAGAGGTTGTGTTTG+TGG - chr5.3:7449792-7449811 MS.gene21474:intron 45.0%
! AATTACCAGGCCAGGTTTTC+CGG + chr5.3:7449944-7449963 None:intergenic 45.0%
!! CAAAGCGCTCTGGCTTTAAA+TGG - chr5.3:7449432-7449451 MS.gene21474:intron 45.0%
!! CGGAGCTGTTGAAAGTGTTA+AGG - chr5.3:7449858-7449877 MS.gene21474:intron 45.0%
ACCTGGCGGTTCAGTTACAA+AGG - chr5.3:7449825-7449844 MS.gene21474:intron 50.0%
CCTGGCGGTTCAGTTACAAA+GGG - chr5.3:7449826-7449845 MS.gene21474:intron 50.0%
GAAGGCTCAGTAGCCACTAT+TGG - chr5.3:7449340-7449359 MS.gene21474:intron 50.0%
GATAAGCTCACCGGAAAACC+TGG - chr5.3:7449931-7449950 MS.gene21474:intron 50.0%
GTACTCCTTTGCACCCAACA+AGG + chr5.3:7450368-7450387 None:intergenic 50.0%
TGGTATCACTGACCATGCTC+AGG - chr5.3:7449360-7449379 MS.gene21474:intron 50.0%
TTTGTGGAGCTTCCAGAACC+TGG - chr5.3:7449808-7449827 MS.gene21474:intron 50.0%
! TAAAGCCAGAGCGCTTTGCA+TGG + chr5.3:7449430-7449449 None:intergenic 50.0%
! TATGTCCATGCAAAGCGCTC+TGG - chr5.3:7449422-7449441 MS.gene21474:intron 50.0%
! TGTTGGTTGCAGGACCATCT+TGG - chr5.3:7449775-7449794 MS.gene21474:intron 50.0%
CCCTTTGTAACTGAACCGCC+AGG + chr5.3:7449829-7449848 None:intergenic 55.0%
GTAACTGAACCGCCAGGTTC+TGG + chr5.3:7449823-7449842 None:intergenic 55.0%
GTTGCAGGACCATCTTGGAG+AGG - chr5.3:7449780-7449799 MS.gene21474:intron 55.0%
GTGGAGCTTCCAGAACCTGG+CGG - chr5.3:7449811-7449830 MS.gene21474:intron 60.0%
GCTCACCGGAAAACCTGGCC+TGG - chr5.3:7449936-7449955 MS.gene21474:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 7449094 7450423 7449094 ID=MS.gene21474
chr5.3 mRNA 7449094 7450423 7449094 ID=MS.gene21474.t1;Parent=MS.gene21474
chr5.3 exon 7450312 7450423 7450312 ID=MS.gene21474.t1.exon1;Parent=MS.gene21474.t1
chr5.3 CDS 7450312 7450423 7450312 ID=cds.MS.gene21474.t1;Parent=MS.gene21474.t1
chr5.3 exon 7450136 7450224 7450136 ID=MS.gene21474.t1.exon2;Parent=MS.gene21474.t1
chr5.3 CDS 7450136 7450224 7450136 ID=cds.MS.gene21474.t1;Parent=MS.gene21474.t1
chr5.3 exon 7449560 7449730 7449560 ID=MS.gene21474.t1.exon3;Parent=MS.gene21474.t1
chr5.3 CDS 7449560 7449730 7449560 ID=cds.MS.gene21474.t1;Parent=MS.gene21474.t1
chr5.3 exon 7449094 7449222 7449094 ID=MS.gene21474.t1.exon4;Parent=MS.gene21474.t1
chr5.3 CDS 7449094 7449222 7449094 ID=cds.MS.gene21474.t1;Parent=MS.gene21474.t1
Gene Sequence

>MS.gene21474

ATGGCACTGAGGATGTGGGCTTCTTCAACTGCCAATGCTCTCAAACTCTCTTCTTCTTCCTCAAGATTACATCTCTCCCCTCCTTTTTCCATCTCCAGATGTTTCTCAACTGGTATCCTTTCATATATACCCTCCTTTTTCCTATCAAGTTGAATGAATGTGGAAACATATATGTATGTATTTAAATAAATTTTTGCAGTGTTGGATGGACTCAAGTATGCATCTTCACATGAATGGGTGAAGCATGAAGGCTCAGTAGCCACTATTGGTATCACTGACCATGCTCAGGTTAGTTAACTGCACCTCTACTCCTTAACATTCTCCACAATATGTCCATGCAAAGCGCTCTGGCTTTAAATGGCCCCCACAAGTGAACTGTGAGATTGAACTATACGAATTAGTCTGTTTTTGGGTCGGATACAGAGTTTCAAAAAAAAAAAAAAAAACTCAACTGAATTGTGTATGTGTTTCTAGTTGTGTGCGCATGTACTTTGAATTAGACTTTAATTACTAGGACTTATAAGTGAACTACATAATTCGACTGAATTGAGCTAAGAGTTAAATGAGGAAAATGATCGAGATTTAAACTCCGACAAAAGATAAAATACTAGCATCAAACTAATATAATAACATAATCATTACAACTTAAGTGAAGGCTGAAACTGTTTAATTAATTGCACTTGTTGGTTGCAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGCGGTTCAGTTACAAAGGGTAACGGTTTCGGAGCTGTTGAAAGTGTTAAGGCAACAAGTGATGTAAACTCTCCAATATCTGGTGAGATTGTTGAGGTTAATGATAAGCTCACCGGAAAACCTGGCCTGGTAATTCATTCATTAACTCACTCTCATTTTTAGGACTAGAAGAAGATTAATTACTGATTGAATATAAGAGCTTATGAATAAGTTGTTTTAATTTAATTTAAAATCAAAATAGAGAATAATAAACTCTGCAAAGCTGAAGACATAAGTGAACTAGAGGGGTACATTTATGAAAAATAAGCGGAAAACAACTTAGAAACATATTATAAGCTATTTACACAAATGTTTATGTCATGAGTCATGACTTATGAAATAAGTCAACATTGAAATAAGTCTTTAGATTTATATTAGTAGCTTACACATGTTCATACTTATTTATTTTTACTTTTGTGACTTGAAGATCAACTCAAGCCCATATGAAGATGGATGGATGATCAAAATAAAGCCAAGTGATCCATCTGAATTAGAATCCTTGTTGGGTGCAAAGGAGTACACAAAATTTTGTGAGGAAGAAGATGCTGCACATTGA

Protein sequence

>MS.gene21474.t1

MALRMWASSTANALKLSSSSSRLHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGSVATIGITDHAQDHLGEVVFVELPEPGGSVTKGNGFGAVESVKATSDVNSPISGEIVEVNDKLTGKPGLINSSPYEDGWMIKIKPSDPSELESLLGAKEYTKFCEEEDAAH