AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09661


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09661.t1 MTR_5g014260 99.398 166 1 0 1 166 1 166 1.36e-120 337
MS.gene09661.t1 MTR_5g014260 99.200 125 1 0 1 125 1 125 2.29e-87 252
MS.gene09661.t1 MTR_4g084110 59.880 167 54 3 1 166 1 155 2.82e-69 207
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09661.t1 AT1G32470 83.133 166 28 0 1 166 1 166 4.67e-94 270
MS.gene09661.t1 AT2G35370 83.133 166 27 1 1 166 1 165 2.24e-89 258
MS.gene09661.t1 AT2G35120 58.929 168 55 3 1 166 1 156 1.52e-64 195

Find 46 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTAACTGAACCGCCAGGTTC+TGG 0.305688 5.2:+6243651 None:intergenic
ATCATCCATCCATCTTCATA+TGG 0.339685 5.2:+6243168 None:intergenic
GTTACAAAGGGTAACGGTTT+CGG 0.348504 5.2:-6243633 MS.gene09661:CDS
AAACATCTGGAGATGGAAAA+AGG 0.352185 5.2:+6244304 None:intergenic
TCAACAATCTCACCAGATAT+TGG 0.373303 5.2:+6243570 None:intergenic
ATCTCCAGATGTTTCTCAAC+TGG 0.379569 5.2:-6244295 MS.gene09661:intron
TCTTGGAGAGGTTGTGTTTG+TGG 0.407524 5.2:-6243679 MS.gene09661:CDS
CGGAGCTGTTGAAAGTGTTA+AGG 0.409242 5.2:-6243613 MS.gene09661:CDS
GATGTAAACTCTCCAATATC+TGG 0.410884 5.2:-6243582 MS.gene09661:CDS
ATCTGAATTAGAAGCCTTGT+TGG 0.425384 5.2:-6243124 MS.gene09661:CDS
AGTATGCATCTTCACATGAA+TGG 0.426720 5.2:-6244171 MS.gene09661:CDS
TAACAATGGCACTGAGGATG+TGG 0.450009 5.2:-6244390 None:intergenic
AGAAGAGAGTTTGAGAGCAT+TGG 0.453540 5.2:+6244354 None:intergenic
GATAAGCTCACCGGAAAACC+TGG 0.464278 5.2:-6243540 MS.gene09661:CDS
TGTTGGTTGCAGGACCATCT+TGG 0.467787 5.2:-6243696 MS.gene09661:intron
GAAGGCTCAGTAGCCACTAT+TGG 0.468590 5.2:-6244139 MS.gene09661:CDS
TCTGAATTAGAAGCCTTGTT+GGG 0.485713 5.2:-6243123 MS.gene09661:CDS
TCATCCATCCATCTTCATAT+GGG 0.487407 5.2:+6243169 None:intergenic
AATACCAGTTGAGAAACATC+TGG 0.490504 5.2:+6244291 None:intergenic
GTATGCATCTTCACATGAAT+GGG 0.495142 5.2:-6244170 MS.gene09661:CDS
AGAAGCCTTGTTGGGTGCAA+AGG 0.506140 5.2:-6243115 MS.gene09661:CDS
AATGAATGAATTACCAGGCC+AGG 0.510526 5.2:+6243522 None:intergenic
TTTGTGGAGCTTCCAGAACC+TGG 0.512073 5.2:-6243663 MS.gene09661:CDS
TCTAATTCAGATGGATCACT+TGG 0.513292 5.2:+6243135 None:intergenic
TGGTATCACTGACCATGCTC+AGG 0.528384 5.2:-6244119 MS.gene09661:intron
GCTCACCGGAAAACCTGGCC+TGG 0.529026 5.2:-6243535 MS.gene09661:intron
AACTCAAGCCCATATGAAGA+TGG 0.530377 5.2:-6243177 MS.gene09661:CDS
AGGGGAGAGATGTAATCTTG+AGG 0.555174 5.2:+6244327 None:intergenic
CATGAATGGGTGAAGCATGA+AGG 0.562003 5.2:-6244157 MS.gene09661:CDS
CCCTTTGTAACTGAACCGCC+AGG 0.564034 5.2:+6243645 None:intergenic
CAAGCCCATATGAAGATGGA+TGG 0.567565 5.2:-6243173 MS.gene09661:CDS
AACAAGGCTTCTAATTCAGA+TGG 0.567704 5.2:+6243126 None:intergenic
ACCTGGCGGTTCAGTTACAA+AGG 0.580268 5.2:-6243646 MS.gene09661:CDS
AGTTGAGAAACATCTGGAGA+TGG 0.581853 5.2:+6244297 None:intergenic
GTTGCAGGACCATCTTGGAG+AGG 0.582318 5.2:-6243691 MS.gene09661:intron
GGTTCAGTTACAAAGGGTAA+CGG 0.588941 5.2:-6243639 MS.gene09661:CDS
CAAACACAACCTCTCCAAGA+TGG 0.593260 5.2:+6243682 None:intergenic
GAGGTTAATGATAAGCTCAC+CGG 0.595144 5.2:-6243549 MS.gene09661:CDS
AATATCTGGTGAGATTGTTG+AGG 0.595757 5.2:-6243568 MS.gene09661:CDS
AACAATGGCACTGAGGATGT+GGG 0.614850 5.2:-6244389 None:intergenic
CCTGGCGGTTCAGTTACAAA+GGG 0.619186 5.2:-6243645 MS.gene09661:CDS
ATGGTCAGTGATACCAATAG+TGG 0.628719 5.2:+6244126 None:intergenic
GTACTCCTTTGCACCCAACA+AGG 0.634212 5.2:+6243110 None:intergenic
GCAGTTAACTAACCTGAGCA+TGG 0.648601 5.2:+6244107 None:intergenic
GTGGAGCTTCCAGAACCTGG+CGG 0.691995 5.2:-6243660 MS.gene09661:CDS
GAAGCTTAACAATGGCACTG+AGG 0.716107 5.2:-6244396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTACATTTATGAAAAATAAG+CGG - chr5.2:6244104-6244123 MS.gene09661:intron 20.0%
!!! ATTCGAATTAGTTTGTTTTT+GGG - chr5.2:6243464-6243483 MS.gene09661:intron 20.0%
!!! TATATAAATTTTTGCAGTGT+TGG - chr5.2:6243256-6243275 MS.gene09661:intron 20.0%
!!! TATTCGAATTAGTTTGTTTT+TGG - chr5.2:6243463-6243482 MS.gene09661:intron 20.0%
! AATCATTACAACTTAAGTGA+AGG - chr5.2:6243715-6243734 MS.gene09661:intron 25.0%
! GTTTAATTAATTGCACTTGT+TGG - chr5.2:6243746-6243765 MS.gene09661:intron 25.0%
! TGAATTAGACTTTAATTGCT+AGG - chr5.2:6243574-6243593 MS.gene09661:CDS 25.0%
!! GCTAGTATTTTATCTTTTGT+CGG + chr5.2:6243674-6243693 None:intergenic 25.0%
!!! ATTAACTCACTCTCATTTTT+AGG - chr5.2:6243957-6243976 MS.gene09661:intron 25.0%
ATTGAGCTAAGAGTTAAATG+AGG - chr5.2:6243627-6243646 MS.gene09661:CDS 30.0%
CATTCAACTTGATAGGAAAA+AGG + chr5.2:6243210-6243229 None:intergenic 30.0%
GAGTTAATGAATGAATTACC+AGG + chr5.2:6243945-6243964 None:intergenic 30.0%
!! TAAATTTTTGCAGTGTTGGA+TGG - chr5.2:6243260-6243279 MS.gene09661:intron 30.0%
!!! GAATTAGTTTGTTTTTGGGT+CGG - chr5.2:6243468-6243487 MS.gene09661:intron 30.0%
AAACATCTGGAGATGGAAAA+AGG + chr5.2:6243158-6243177 None:intergenic 35.0%
AACAAGGCTTCTAATTCAGA+TGG + chr5.2:6244336-6244355 None:intergenic 35.0%
AAGACATAAGTGAACTAGAG+GGG - chr5.2:6244082-6244101 MS.gene09661:intron 35.0%
AATACCAGTTGAGAAACATC+TGG + chr5.2:6243171-6243190 None:intergenic 35.0%
AATATCTGGTGAGATTGTTG+AGG - chr5.2:6243891-6243910 MS.gene09661:intron 35.0%
ACATAGTGTGGAGAATGTTA+AGG + chr5.2:6243387-6243406 None:intergenic 35.0%
AGTATGCATCTTCACATGAA+TGG - chr5.2:6243288-6243307 MS.gene09661:intron 35.0%
ATCATCCATCCATCTTCATA+TGG + chr5.2:6244294-6244313 None:intergenic 35.0%
CAACTTGATAGGAAAAAGGA+GGG + chr5.2:6243206-6243225 None:intergenic 35.0%
CCACATTCATTCAACTTGAT+AGG + chr5.2:6243217-6243236 None:intergenic 35.0%
GAAGACATAAGTGAACTAGA+GGG - chr5.2:6244081-6244100 MS.gene09661:intron 35.0%
GATGTAAACTCTCCAATATC+TGG - chr5.2:6243877-6243896 MS.gene09661:intron 35.0%
GTATGCATCTTCACATGAAT+GGG - chr5.2:6243289-6243308 MS.gene09661:intron 35.0%
TCAACAATCTCACCAGATAT+TGG + chr5.2:6243892-6243911 None:intergenic 35.0%
TCAACTTGATAGGAAAAAGG+AGG + chr5.2:6243207-6243226 None:intergenic 35.0%
TCATCCATCCATCTTCATAT+GGG + chr5.2:6244293-6244312 None:intergenic 35.0%
TCTAATTCAGATGGATCACT+TGG + chr5.2:6244327-6244346 None:intergenic 35.0%
TGAAGACATAAGTGAACTAG+AGG - chr5.2:6244080-6244099 MS.gene09661:intron 35.0%
! ATCTGAATTAGAAGCCTTGT+TGG - chr5.2:6244335-6244354 MS.gene09661:CDS 35.0%
! GGAGTACACAAAATTTTGTG+AGG - chr5.2:6244365-6244384 MS.gene09661:CDS 35.0%
!! CCTATCAAGTTGAATGAATG+TGG - chr5.2:6243214-6243233 MS.gene09661:intron 35.0%
!! TCTGAATTAGAAGCCTTGTT+GGG - chr5.2:6244336-6244355 MS.gene09661:CDS 35.0%
AACTCAAGCCCATATGAAGA+TGG - chr5.2:6244282-6244301 MS.gene09661:intron 40.0%
AATCTCACAGTTCACTTGTG+GGG + chr5.2:6243439-6243458 None:intergenic 40.0%
AATGAATGAATTACCAGGCC+AGG + chr5.2:6243940-6243959 None:intergenic 40.0%
AGTTGAGAAACATCTGGAGA+TGG + chr5.2:6243165-6243184 None:intergenic 40.0%
ATCTCCAGATGTTTCTCAAC+TGG - chr5.2:6243164-6243183 MS.gene09661:CDS 40.0%
ATCTGGAGATGGAAAAAGGA+GGG + chr5.2:6243154-6243173 None:intergenic 40.0%
ATGGTCAGTGATACCAATAG+TGG + chr5.2:6243336-6243355 None:intergenic 40.0%
CAATCTCACAGTTCACTTGT+GGG + chr5.2:6243440-6243459 None:intergenic 40.0%
GAGGTTAATGATAAGCTCAC+CGG - chr5.2:6243910-6243929 MS.gene09661:intron 40.0%
GGTTCAGTTACAAAGGGTAA+CGG - chr5.2:6243820-6243839 MS.gene09661:intron 40.0%
TAGTGTGGAGAATGTTAAGG+AGG + chr5.2:6243384-6243403 None:intergenic 40.0%
TCAATCTCACAGTTCACTTG+TGG + chr5.2:6243441-6243460 None:intergenic 40.0%
! AGAAGAGAGTTTGAGAGCAT+TGG + chr5.2:6243108-6243127 None:intergenic 40.0%
! GTTACAAAGGGTAACGGTTT+CGG - chr5.2:6243826-6243845 MS.gene09661:intron 40.0%
! TAATTGCACTTGTTGGTTGC+AGG - chr5.2:6243753-6243772 MS.gene09661:intron 40.0%
AGGGGAGAGATGTAATCTTG+AGG + chr5.2:6243135-6243154 None:intergenic 45.0%
ATCTCACAGTTCACTTGTGG+GGG + chr5.2:6243438-6243457 None:intergenic 45.0%
CAAACACAACCTCTCCAAGA+TGG + chr5.2:6243780-6243799 None:intergenic 45.0%
CAAGCCCATATGAAGATGGA+TGG - chr5.2:6244286-6244305 MS.gene09661:intron 45.0%
CATCTGGAGATGGAAAAAGG+AGG + chr5.2:6243155-6243174 None:intergenic 45.0%
CATGAATGGGTGAAGCATGA+AGG - chr5.2:6243302-6243321 MS.gene09661:intron 45.0%
GCAGTTAACTAACCTGAGCA+TGG + chr5.2:6243355-6243374 None:intergenic 45.0%
TCTGGAGATGGAAAAAGGAG+GGG + chr5.2:6243153-6243172 None:intergenic 45.0%
TCTTGGAGAGGTTGTGTTTG+TGG - chr5.2:6243780-6243799 MS.gene09661:intron 45.0%
TGGAGAATGTTAAGGAGGAG+AGG + chr5.2:6243379-6243398 None:intergenic 45.0%
! AATTACCAGGCCAGGTTTTC+CGG + chr5.2:6243932-6243951 None:intergenic 45.0%
!! CAAAGCGCTCTGGCTTTAAA+TGG - chr5.2:6243412-6243431 MS.gene09661:intron 45.0%
!! CGGAGCTGTTGAAAGTGTTA+AGG - chr5.2:6243846-6243865 MS.gene09661:intron 45.0%
ACCTGGCGGTTCAGTTACAA+AGG - chr5.2:6243813-6243832 MS.gene09661:intron 50.0%
CCTGGCGGTTCAGTTACAAA+GGG - chr5.2:6243814-6243833 MS.gene09661:intron 50.0%
CGCTTTGCATGGACATAGTG+TGG + chr5.2:6243399-6243418 None:intergenic 50.0%
GAAGGCTCAGTAGCCACTAT+TGG - chr5.2:6243320-6243339 MS.gene09661:intron 50.0%
GATAAGCTCACCGGAAAACC+TGG - chr5.2:6243919-6243938 MS.gene09661:intron 50.0%
GTACTCCTTTGCACCCAACA+AGG + chr5.2:6244352-6244371 None:intergenic 50.0%
TGGTATCACTGACCATGCTC+AGG - chr5.2:6243340-6243359 MS.gene09661:intron 50.0%
TTTGTGGAGCTTCCAGAACC+TGG - chr5.2:6243796-6243815 MS.gene09661:intron 50.0%
! TAAAGCCAGAGCGCTTTGCA+TGG + chr5.2:6243410-6243429 None:intergenic 50.0%
! TATGTCCATGCAAAGCGCTC+TGG - chr5.2:6243402-6243421 MS.gene09661:intron 50.0%
! TGTTGGTTGCAGGACCATCT+TGG - chr5.2:6243763-6243782 MS.gene09661:intron 50.0%
!! AGAAGCCTTGTTGGGTGCAA+AGG - chr5.2:6244344-6244363 MS.gene09661:CDS 50.0%
CCCTTTGTAACTGAACCGCC+AGG + chr5.2:6243817-6243836 None:intergenic 55.0%
GTAACTGAACCGCCAGGTTC+TGG + chr5.2:6243811-6243830 None:intergenic 55.0%
GTTGCAGGACCATCTTGGAG+AGG - chr5.2:6243768-6243787 MS.gene09661:intron 55.0%
GTGGAGCTTCCAGAACCTGG+CGG - chr5.2:6243799-6243818 MS.gene09661:intron 60.0%
GCTCACCGGAAAACCTGGCC+TGG - chr5.2:6243924-6243943 MS.gene09661:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 6243074 6244407 6243074 ID=MS.gene09661
chr5.2 mRNA 6243074 6244407 6243074 ID=MS.gene09661.t1;Parent=MS.gene09661
chr5.2 exon 6244296 6244407 6244296 ID=MS.gene09661.t1.exon1;Parent=MS.gene09661.t1
chr5.2 CDS 6244296 6244407 6244296 ID=cds.MS.gene09661.t1;Parent=MS.gene09661.t1
chr5.2 exon 6244120 6244208 6244120 ID=MS.gene09661.t1.exon2;Parent=MS.gene09661.t1
chr5.2 CDS 6244120 6244208 6244120 ID=cds.MS.gene09661.t1;Parent=MS.gene09661.t1
chr5.2 exon 6243536 6243706 6243536 ID=MS.gene09661.t1.exon3;Parent=MS.gene09661.t1
chr5.2 CDS 6243536 6243706 6243536 ID=cds.MS.gene09661.t1;Parent=MS.gene09661.t1
chr5.2 exon 6243074 6243202 6243074 ID=MS.gene09661.t1.exon4;Parent=MS.gene09661.t1
chr5.2 CDS 6243074 6243202 6243074 ID=cds.MS.gene09661.t1;Parent=MS.gene09661.t1
Gene Sequence

>MS.gene09661

ATGGCACTGAGGATGTGGGCTTCTTCAACTGCCAATGCTCTCAAACTCTCTTCTTCTTCCTCAAGATTACATCTCTCCCCTCCTTTTTCCATCTCCAGATGTTTCTCAACTGGTATTCTTTCATATATACCCTCCTTTTTCCTATCAAGTTGAATGAATGTGGAAACATATATGTATGTATTTATATAAATTTTTGCAGTGTTGGATGGACTCAAGTATGCATCTTCACATGAATGGGTGAAGCATGAAGGCTCAGTAGCCACTATTGGTATCACTGACCATGCTCAGGTTAGTTAACTGCACCTCTCCTCCTTAACATTCTCCACACTATGTCCATGCAAAGCGCTCTGGCTTTAAATGGCCCCCACAAGTGAACTGTGAGATTGAACTATTCGAATTAGTTTGTTTTTGGGTCGGATACAGAGTTTTAAAAAAAAAAAAAAAAAAAAAAAAACTCAACTGAATTGTGTATGTGTTTCTAGTTGTGTGCGCATGTACTTTGAATTAGACTTTAATTGCTAGGACTTATAAGTGAACTACATAATTCGACTGAATTGAGCTAAGAGTTAAATGAGGAAAATGATCGAGATTTAAACTCCGACAAAAGATAAAATACTAGCATCAAACTAATATAATAACATAATCATTACAACTTAAGTGAAGGCTGAAACTGTTTAATTAATTGCACTTGTTGGTTGCAGGACCATCTTGGAGAGGTTGTGTTTGTGGAGCTTCCAGAACCTGGCGGTTCAGTTACAAAGGGTAACGGTTTCGGAGCTGTTGAAAGTGTTAAGGCAACAAGTGATGTAAACTCTCCAATATCTGGTGAGATTGTTGAGGTTAATGATAAGCTCACCGGAAAACCTGGCCTGGTAATTCATTCATTAACTCACTCTCATTTTTAGGACTAGAAGAAGATTAATTACTGATTGAATATAAGAGCTTATGAATAAGTTGTTTTAATTTAATTTAAAATCAAAATAGAGAATAATAAACTCTGCAAAGCTGAAGACATAAGTGAACTAGAGGGGTACATTTATGAAAAATAAGCGGAAAACAACTTAGAAACATATTATAAGCTATTTACACAAATGTTTATGTCATGATGACTTATGAAATAAGTCAACATTGAAATAAGTCTTTAGATTTATATTAGTAGCTTACACATGTTCATACTTATTTATTTTTACTTTTGTGACTTGAAGATCAACTCAAGCCCATATGAAGATGGATGGATGATCAAAATAAAGCCAAGTGATCCATCTGAATTAGAAGCCTTGTTGGGTGCAAAGGAGTACACAAAATTTTGTGAGGAAGAAGATGCTGCACATTGA

Protein sequence

>MS.gene09661.t1

MALRMWASSTANALKLSSSSSRLHLSPPFSISRCFSTVLDGLKYASSHEWVKHEGSVATIGITDHAQDHLGEVVFVELPEPGGSVTKGNGFGAVESVKATSDVNSPISGEIVEVNDKLTGKPGLINSSPYEDGWMIKIKPSDPSELEALLGAKEYTKFCEEEDAAH