AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89246


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89246.t1 MTR_7g065720 96.444 225 8 0 1 225 1 225 3.01e-160 442
MS.gene89246.t1 MTR_7g065660 76.786 224 51 1 1 224 1 223 2.70e-127 358
MS.gene89246.t1 MTR_7g065740 75.000 224 56 0 1 224 1 224 1.04e-125 355
MS.gene89246.t1 MTR_7g065750 75.221 226 55 1 1 225 1 226 8.07e-124 350
MS.gene89246.t1 MTR_7g065600 69.778 225 67 1 1 225 1 224 6.92e-114 325
MS.gene89246.t1 MTR_7g065640 72.321 224 43 2 1 224 1 205 1.85e-113 323
MS.gene89246.t1 MTR_7g065590 67.556 225 72 1 1 225 1 224 6.66e-112 320
MS.gene89246.t1 MTR_7g065290 70.222 225 66 1 1 225 1 224 9.88e-112 319
MS.gene89246.t1 MTR_7g065265 67.556 225 72 1 1 225 1 224 9.39e-106 304
MS.gene89246.t1 MTR_4g019790 65.625 224 76 1 1 224 1 223 4.21e-105 303
MS.gene89246.t1 MTR_7g065270 66.667 225 74 1 1 225 1 224 3.09e-104 300
MS.gene89246.t1 MTR_7g065630 68.304 224 66 2 1 224 19 237 1.12e-103 300
MS.gene89246.t1 MTR_7g065230 65.778 225 71 2 1 225 1 219 8.62e-100 289
MS.gene89246.t1 MTR_7g065260 62.832 226 82 2 1 225 8 232 1.85e-97 283
MS.gene89246.t1 MTR_4g019780 63.303 218 76 3 1 216 1 216 1.85e-96 280
MS.gene89246.t1 MTR_7g065710 65.865 208 66 2 8 215 5 207 5.99e-96 279
MS.gene89246.t1 MTR_7g065680 64.574 223 74 2 1 223 1 218 1.09e-89 263
MS.gene89246.t1 MTR_7g065700 66.512 215 67 2 1 215 1 210 8.91e-89 261
MS.gene89246.t1 MTR_8g087425 44.292 219 119 2 4 221 2 218 3.83e-66 204
MS.gene89246.t1 MTR_3g099757 45.701 221 117 2 5 224 3 221 2.94e-65 201
MS.gene89246.t1 MTR_6g080440 46.890 209 108 2 7 214 3 209 4.65e-65 201
MS.gene89246.t1 MTR_8g087410 47.664 214 108 3 1 213 1 211 7.69e-64 198
MS.gene89246.t1 MTR_1g090150 43.111 225 114 4 6 223 2 219 8.12e-61 190
MS.gene89246.t1 MTR_1g115195 45.972 211 112 1 7 215 3 213 1.52e-60 189
MS.gene89246.t1 MTR_1g090070 45.495 222 115 3 7 224 9 228 6.23e-60 188
MS.gene89246.t1 MTR_1g090060 44.144 222 119 3 7 225 3 222 1.04e-55 177
MS.gene89246.t1 MTR_4g059730 44.393 214 114 2 6 216 3 214 1.12e-55 177
MS.gene89246.t1 MTR_1g090100 44.091 220 118 3 8 224 3 220 5.58e-54 172
MS.gene89246.t1 MTR_1g090090 44.762 210 111 2 7 213 3 210 9.82e-54 172
MS.gene89246.t1 MTR_5g037380 44.344 221 112 4 1 213 1 218 1.38e-53 172
MS.gene89246.t1 MTR_5g040430 40.807 223 123 3 1 216 1 221 1.84e-53 171
MS.gene89246.t1 MTR_8g061950 43.182 220 116 4 5 216 3 221 2.51e-50 164
MS.gene89246.t1 MTR_2g070150 42.718 206 110 5 6 208 3 203 4.59e-47 154
MS.gene89246.t1 MTR_2g070200 42.439 205 113 4 6 208 3 204 9.66e-47 154
MS.gene89246.t1 MTR_2g070180 40.488 205 116 4 6 208 3 203 2.93e-45 150
MS.gene89246.t1 MTR_3g467420 41.827 208 115 5 8 213 10 213 3.57e-45 150
MS.gene89246.t1 MTR_2g070120 42.029 207 114 5 6 210 3 205 4.19e-45 150
MS.gene89246.t1 MTR_2g070130 40.976 205 115 4 6 208 3 203 4.62e-45 150
MS.gene89246.t1 MTR_2g070140 41.837 196 106 6 21 213 2 192 7.00e-45 149
MS.gene89246.t1 MTR_3g467430 40.094 212 121 5 4 213 6 213 4.52e-44 147
MS.gene89246.t1 MTR_2g070210 40.566 212 118 6 6 213 3 210 2.47e-43 145
MS.gene89246.t1 MTR_8g056940 43.915 189 100 4 6 192 3 187 3.08e-42 142
MS.gene89246.t1 MTR_4g124130 39.091 220 127 5 5 221 2 217 2.00e-41 140
MS.gene89246.t1 MTR_5g076900 37.559 213 127 5 3 213 2 210 1.63e-39 135
MS.gene89246.t1 MTR_2g070060 36.321 212 123 5 7 213 4 208 2.30e-39 135
MS.gene89246.t1 MTR_2g070070 36.406 217 132 4 7 221 4 216 7.36e-39 134
MS.gene89246.t1 MTR_2g070110 38.278 209 122 4 7 213 4 207 5.07e-37 129
MS.gene89246.t1 MTR_0186s0030 48.227 141 70 2 6 145 3 141 1.35e-34 120
MS.gene89246.t1 MTR_2g072120 49.153 59 29 1 51 108 2 60 4.21e-14 65.5
MS.gene89246.t1 MTR_5g037390 45.205 73 37 1 31 100 11 83 1.10e-12 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89246.t1 AT3G09270 50.667 225 105 5 4 224 2 224 9.69e-76 228
MS.gene89246.t1 AT2G29420 49.545 220 108 2 3 221 5 222 1.07e-74 226
MS.gene89246.t1 AT2G29490 48.095 210 105 3 1 208 1 208 4.41e-60 188
MS.gene89246.t1 AT2G29480 45.714 210 110 3 1 208 1 208 7.29e-56 177
MS.gene89246.t1 AT2G29450 47.115 208 107 3 3 208 2 208 9.47e-55 175
MS.gene89246.t1 AT2G29460 43.498 223 122 3 1 221 1 221 1.10e-54 174
MS.gene89246.t1 AT1G10370 45.192 208 106 3 5 205 3 209 1.12e-54 175
MS.gene89246.t1 AT2G29440 46.190 210 108 4 1 208 1 207 2.84e-53 171
MS.gene89246.t1 AT2G29470 43.602 211 114 4 1 208 1 209 5.65e-53 170
MS.gene89246.t1 AT1G27130 41.593 226 122 5 1 218 1 224 8.35e-52 167
MS.gene89246.t1 AT1G59700 46.411 209 103 5 5 205 4 211 2.34e-51 166
MS.gene89246.t1 AT1G10360 42.453 212 112 4 1 205 1 209 2.78e-51 166
MS.gene89246.t1 AT1G59670 44.019 209 108 4 5 205 4 211 1.43e-50 164
MS.gene89246.t1 AT1G78340 42.857 210 114 4 6 213 3 208 5.73e-49 160
MS.gene89246.t1 AT1G69930 39.111 225 130 3 2 220 7 230 4.42e-48 158
MS.gene89246.t1 AT1G53680 38.967 213 126 3 1 211 1 211 4.21e-47 155
MS.gene89246.t1 AT1G78370 40.291 206 117 4 10 213 7 208 4.61e-47 155
MS.gene89246.t1 AT1G69920 40.465 215 117 5 1 205 26 239 6.71e-47 155
MS.gene89246.t1 AT1G78380 43.284 201 108 4 7 205 4 200 6.83e-47 154
MS.gene89246.t1 AT1G78320 40.930 215 121 5 5 217 2 212 1.64e-46 154
MS.gene89246.t1 AT1G17170 39.908 218 124 4 6 221 3 215 1.31e-45 151
MS.gene89246.t1 AT1G27140 38.961 231 130 5 1 222 1 229 4.04e-45 150
MS.gene89246.t1 AT5G62480 38.288 222 125 4 5 216 6 225 3.62e-44 148
MS.gene89246.t1 AT1G17180 40.000 210 120 5 6 213 3 208 3.94e-43 145
MS.gene89246.t1 AT1G78360 40.476 210 118 5 7 213 4 209 5.16e-42 142
MS.gene89246.t1 AT3G43800 38.462 208 118 6 4 205 2 205 4.86e-39 134
MS.gene89246.t1 AT1G74590 33.778 225 141 4 1 219 1 223 2.34e-38 133
MS.gene89246.t1 AT1G17190 38.140 215 125 5 1 213 1 209 4.97e-37 129
MS.gene89246.t1 AT1G78320 34.579 214 109 5 5 217 2 185 2.26e-32 116
MS.gene89246.t1 AT5G62480 30.769 221 117 4 5 216 6 199 1.55e-28 107
MS.gene89246.t1 AT5G62480 30.317 221 117 4 5 216 6 198 1.21e-27 105

Find 40 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGCTCCACTTGGAAAGTT+TGG 0.246363 7.1:-36920657 None:intergenic
AAAGAGCCTTGGCTAGATTT+TGG 0.274128 7.1:+36920100 MS.gene89246:CDS
AATCACAAGTGATTCTGATA+TGG 0.275550 7.1:-36920019 None:intergenic
GAGCTGAAGGACAAGTTCTT+TGG 0.294063 7.1:+36920527 MS.gene89246:CDS
AAAATCTAGCCAAGGCTCTT+TGG 0.322049 7.1:-36920098 None:intergenic
TGGCTCCACTTGGAAAGTTT+GGG 0.342035 7.1:-36920656 None:intergenic
TTGGATGGTTGAGAAATTCT+TGG 0.345416 7.1:-36920676 None:intergenic
TCTCAACCATCCAATTGTTA+AGG 0.377126 7.1:+36920685 MS.gene89246:CDS
CCTTTGATTCAAGACATAAC+AGG 0.388085 7.1:+36920605 MS.gene89246:CDS
TTCTTTGGAGGAGAGGAGAT+TGG 0.390435 7.1:+36920542 MS.gene89246:CDS
GTGAAGCTATTGGGAGCTAT+AGG 0.394371 7.1:+36919844 MS.gene89246:CDS
TGCTTCAATGGCTACAAATC+AGG 0.399394 7.1:+36919816 None:intergenic
CAGGAAGAAGTGAAGCTATT+GGG 0.411817 7.1:+36919835 MS.gene89246:CDS
TCAGGAAGAAGTGAAGCTAT+TGG 0.415262 7.1:+36919834 MS.gene89246:CDS
ATCTGTACTCTACACACAAA+TGG 0.424561 7.1:-36919871 None:intergenic
ACAGATTGCTCTAAAGTTGA+AGG 0.449931 7.1:+36919888 MS.gene89246:CDS
ATCACAAGTGATTCTGATAT+GGG 0.450253 7.1:-36920018 None:intergenic
AGGAGAGGAGATTGGAATTG+TGG 0.454908 7.1:+36920550 MS.gene89246:CDS
GAGAAATTCTTGGCTCCACT+TGG 0.459712 7.1:-36920666 None:intergenic
GCTCTTTGGTATGGATCATA+AGG 0.477384 7.1:-36920084 None:intergenic
CTAGCCAAGGCTCTTTGGTA+TGG 0.477671 7.1:-36920093 None:intergenic
CTGAAGGACAAGTTCTTTGG+AGG 0.491967 7.1:+36920530 MS.gene89246:CDS
CCTGTTATGTCTTGAATCAA+AGG 0.497589 7.1:-36920605 None:intergenic
TCTGTACTCTACACACAAAT+GGG 0.504085 7.1:-36919870 None:intergenic
GAGCGCGAGAAGAATATTGA+AGG 0.504203 7.1:+36920473 MS.gene89246:CDS
TTGAGTATATTGATGAGACT+TGG 0.504629 7.1:+36920043 MS.gene89246:CDS
TATAAACTGGGTTATACTTG+AGG 0.528468 7.1:-36919963 None:intergenic
CAAATTTCTTGAAGAAAACA+TGG 0.532285 7.1:+36919921 MS.gene89246:CDS
GGACAAGTTCTTTGGAGGAG+AGG 0.532885 7.1:+36920535 MS.gene89246:CDS
CTTTCTCATCATCAACGGTA+TGG 0.537106 7.1:-36920451 None:intergenic
CTTTGATTCAAGACATAACA+GGG 0.541148 7.1:+36920606 MS.gene89246:CDS
CTGTTATGTCTTGAATCAAA+GGG 0.548966 7.1:-36920604 None:intergenic
GAATATTGAAGGAACAATTG+AGG 0.577684 7.1:+36920484 MS.gene89246:CDS
TGATCCATACCAAAGAGCCT+TGG 0.577800 7.1:+36920089 MS.gene89246:CDS
GATTCTTGAGAATGAGCTGA+AGG 0.588727 7.1:+36920514 MS.gene89246:CDS
CAGATTGCTCTAAAGTTGAA+GGG 0.593796 7.1:+36919889 MS.gene89246:CDS
GTCTAACTTCATCGACGACA+AGG 0.594451 7.1:+36920122 MS.gene89246:CDS
GTTAGACCAAAATCTAGCCA+AGG 0.639426 7.1:-36920106 None:intergenic
GCGCTCTTTCTCATCATCAA+CGG 0.643070 7.1:-36920456 None:intergenic
AGTGTCCCAAACTTTCCAAG+TGG 0.647650 7.1:+36920651 MS.gene89246:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATACATCATATGTGATTGT+TGG - chr7.1:36920255-36920274 None:intergenic 25.0%
! AATTTACATCTTTGATGTCA+CGG + chr7.1:36920341-36920360 MS.gene89246:intron 25.0%
! CAAATTTCTTGAAGAAAACA+TGG + chr7.1:36919921-36919940 MS.gene89246:CDS 25.0%
!! ACTGGAATCTTTTTATAAAC+TGG - chr7.1:36919979-36919998 None:intergenic 25.0%
!! ATTTACATTAGAAAAGTACG+CGG - chr7.1:36920379-36920398 None:intergenic 25.0%
!! CTGGAATCTTTTTATAAACT+GGG - chr7.1:36919978-36919997 None:intergenic 25.0%
!! TATTTTCCTTAACAATTGGA+TGG - chr7.1:36920694-36920713 None:intergenic 25.0%
!! TTTTCATTGTGAACAAAAAC+TGG - chr7.1:36919997-36920016 None:intergenic 25.0%
AATCACAAGTGATTCTGATA+TGG - chr7.1:36920022-36920041 None:intergenic 30.0%
ATCACAAGTGATTCTGATAT+GGG - chr7.1:36920021-36920040 None:intergenic 30.0%
ATTACATCATACGTGATTGA+TGG + chr7.1:36920202-36920221 MS.gene89246:intron 30.0%
CTGTTATGTCTTGAATCAAA+GGG - chr7.1:36920607-36920626 None:intergenic 30.0%
CTTTGATTCAAGACATAACA+GGG + chr7.1:36920606-36920625 MS.gene89246:CDS 30.0%
GAATATTGAAGGAACAATTG+AGG + chr7.1:36920484-36920503 MS.gene89246:CDS 30.0%
TATAAACTGGGTTATACTTG+AGG - chr7.1:36919966-36919985 None:intergenic 30.0%
TTGAGTATATTGATGAGACT+TGG + chr7.1:36920043-36920062 MS.gene89246:CDS 30.0%
! GGCATATTTTCCTTAACAAT+TGG - chr7.1:36920698-36920717 None:intergenic 30.0%
!! GCTTTGAAATAAGCAAAAAG+AGG - chr7.1:36920731-36920750 None:intergenic 30.0%
ATCTGTACTCTACACACAAA+TGG - chr7.1:36919874-36919893 None:intergenic 35.0%
CCTGTTATGTCTTGAATCAA+AGG - chr7.1:36920608-36920627 None:intergenic 35.0%
CCTTTGATTCAAGACATAAC+AGG + chr7.1:36920605-36920624 MS.gene89246:CDS 35.0%
TAAGCAAAAAGAGGTTCTCT+AGG - chr7.1:36920722-36920741 None:intergenic 35.0%
TCTCAACCATCCAATTGTTA+AGG + chr7.1:36920685-36920704 MS.gene89246:CDS 35.0%
TCTGTACTCTACACACAAAT+GGG - chr7.1:36919873-36919892 None:intergenic 35.0%
TTGGATGGTTGAGAAATTCT+TGG - chr7.1:36920679-36920698 None:intergenic 35.0%
!! ACAGATTGCTCTAAAGTTGA+AGG + chr7.1:36919888-36919907 MS.gene89246:CDS 35.0%
!! CAGATTGCTCTAAAGTTGAA+GGG + chr7.1:36919889-36919908 MS.gene89246:CDS 35.0%
AAAATCTAGCCAAGGCTCTT+TGG - chr7.1:36920101-36920120 None:intergenic 40.0%
CAGGAAGAAGTGAAGCTATT+GGG + chr7.1:36919835-36919854 MS.gene89246:CDS 40.0%
CTTTCTCATCATCAACGGTA+TGG - chr7.1:36920454-36920473 None:intergenic 40.0%
GATTCTTGAGAATGAGCTGA+AGG + chr7.1:36920514-36920533 MS.gene89246:CDS 40.0%
GCTCTTTGGTATGGATCATA+AGG - chr7.1:36920087-36920106 None:intergenic 40.0%
GTTAGACCAAAATCTAGCCA+AGG - chr7.1:36920109-36920128 None:intergenic 40.0%
TCAGGAAGAAGTGAAGCTAT+TGG + chr7.1:36919834-36919853 MS.gene89246:CDS 40.0%
! AAAGAGCCTTGGCTAGATTT+TGG + chr7.1:36920100-36920119 MS.gene89246:CDS 40.0%
! CAGCTGTCTACATAGCTTTT+TGG + chr7.1:36920579-36920598 MS.gene89246:CDS 40.0%
!! GAGGAGAAGTTCACTTTTGT+TGG - chr7.1:36919947-36919966 None:intergenic 40.0%
AGGAGAGGAGATTGGAATTG+TGG + chr7.1:36920550-36920569 MS.gene89246:CDS 45.0%
AGTGTCCCAAACTTTCCAAG+TGG + chr7.1:36920651-36920670 MS.gene89246:CDS 45.0%
CTGAAGGACAAGTTCTTTGG+AGG + chr7.1:36920530-36920549 MS.gene89246:CDS 45.0%
GAGAAATTCTTGGCTCCACT+TGG - chr7.1:36920669-36920688 None:intergenic 45.0%
GAGCGCGAGAAGAATATTGA+AGG + chr7.1:36920473-36920492 MS.gene89246:CDS 45.0%
GAGCTGAAGGACAAGTTCTT+TGG + chr7.1:36920527-36920546 MS.gene89246:CDS 45.0%
GCAAAAAGAGGTTCTCTAGG+AGG - chr7.1:36920719-36920738 None:intergenic 45.0%
GCGCTCTTTCTCATCATCAA+CGG - chr7.1:36920459-36920478 None:intergenic 45.0%
GTCTAACTTCATCGACGACA+AGG + chr7.1:36920122-36920141 MS.gene89246:CDS 45.0%
GTGAAGCTATTGGGAGCTAT+AGG + chr7.1:36919844-36919863 MS.gene89246:CDS 45.0%
TGATCCATACCAAAGAGCCT+TGG + chr7.1:36920089-36920108 MS.gene89246:CDS 45.0%
TGGCTCCACTTGGAAAGTTT+GGG - chr7.1:36920659-36920678 None:intergenic 45.0%
TTGGCTCCACTTGGAAAGTT+TGG - chr7.1:36920660-36920679 None:intergenic 45.0%
! TTCTTTGGAGGAGAGGAGAT+TGG + chr7.1:36920542-36920561 MS.gene89246:CDS 45.0%
CTAGCCAAGGCTCTTTGGTA+TGG - chr7.1:36920096-36920115 None:intergenic 50.0%
! GGACAAGTTCTTTGGAGGAG+AGG + chr7.1:36920535-36920554 MS.gene89246:CDS 50.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 36919823 36920781 36919823 ID=MS.gene89246
chr7.1 mRNA 36919823 36920781 36919823 ID=MS.gene89246.t1;Parent=MS.gene89246
chr7.1 exon 36919823 36920143 36919823 ID=MS.gene89246.t1.exon1;Parent=MS.gene89246.t1
chr7.1 CDS 36919823 36920143 36919823 ID=cds.MS.gene89246.t1;Parent=MS.gene89246.t1
chr7.1 exon 36920425 36920781 36920425 ID=MS.gene89246.t1.exon2;Parent=MS.gene89246.t1
chr7.1 CDS 36920425 36920781 36920425 ID=cds.MS.gene89246.t1;Parent=MS.gene89246.t1
Gene Sequence

>MS.gene89246

ATGGCTACAAATCAGGAAGAAGTGAAGCTATTGGGAGCTATAGGAAGCCCATTTGTGTGTAGAGTACAGATTGCTCTAAAGTTGAAGGGAATTGAATACAAATTTCTTGAAGAAAACATGGCCAACAAAAGTGAACTTCTCCTCAAGTATAACCCAGTTTATAAAAAGATTCCAGTTTTTGTTCACAATGAAAAGCCCATATCAGAATCACTTGTGATTCTTGAGTATATTGATGAGACTTGGAAACAAAATTCAATCTTGCCTTATGATCCATACCAAAGAGCCTTGGCTAGATTTTGGTCTAACTTCATCGACGACAAGGTAAATACGCTGAATTTTAAATTTTAGTCGCGTTAAAATACTCTATTTTATCAAAAAAATTACATCATACGTGATTGATGGAGTTTTAACTGTCTTCGCGTTACAACTCCAACAATCACATATGATGTATTTGTTTTGAGAAAATCAAGTATTTCAAGCGTAAATTACTAATGCAAGACTTATGAATAAAACACTTAAATTTACATCTTTGATGTCACGGCTCAAATTGCAGCCGCGTACTTTTCTAATGTAAATTACTAATAAATTTTTGAAATGATCAGATCATAGCTGCTTCAATAAAAGCTGTCCATACCGTTGATGATGAGAAAGAGCGCGAGAAGAATATTGAAGGAACAATTGAGGCTCTACAGATTCTTGAGAATGAGCTGAAGGACAAGTTCTTTGGAGGAGAGGAGATTGGAATTGTGGATATTGCAGCTGTCTACATAGCTTTTTGGATCCCTTTGATTCAAGACATAACAGGGTTGCAATTGTTGAATGCTGAAAAGTGTCCCAAACTTTCCAAGTGGAGCCAAGAATTTCTCAACCATCCAATTGTTAAGGAAAATATGCCTCCTAGAGAACCTCTTTTTGCTTATTTCAAAGCTCACTATGAGAGCCTCTTTGCTTCAAAATAG

Protein sequence

>MS.gene89246.t1

MATNQEEVKLLGAIGSPFVCRVQIALKLKGIEYKFLEENMANKSELLLKYNPVYKKIPVFVHNEKPISESLVILEYIDETWKQNSILPYDPYQRALARFWSNFIDDKIIAASIKAVHTVDDEKEREKNIEGTIEALQILENELKDKFFGGEEIGIVDIAAVYIAFWIPLIQDITGLQLLNAEKCPKLSKWSQEFLNHPIVKENMPPREPLFAYFKAHYESLFASK