Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90179.t1 | PNY17151.1 | 70.5 | 44 | 5 | 1 | 1 | 36 | 111 | 154 | 5.80E-06 | 59.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90179.t1 | A0A2K3PPF5 | 70.5 | 44 | 5 | 1 | 1 | 36 | 111 | 154 | 4.1e-06 | 59.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene90179 | 0.806709 | 7.21E-50 | -1.69E-46 |
| MS.gene049272 | MS.gene90179 | 0.802252 | 6.13E-49 | -1.69E-46 |
| MS.gene050026 | MS.gene90179 | 0.804969 | 1.67E-49 | -1.69E-46 |
| MS.gene050156 | MS.gene90179 | 0.802365 | 5.81E-49 | -1.69E-46 |
| MS.gene051147 | MS.gene90179 | 0.805388 | 1.37E-49 | -1.69E-46 |
| MS.gene053166 | MS.gene90179 | 0.811498 | 6.79E-51 | -1.69E-46 |
| MS.gene053974 | MS.gene90179 | 0.80037 | 1.49E-48 | -1.69E-46 |
| MS.gene054291 | MS.gene90179 | -0.805561 | 1.26E-49 | -1.69E-46 |
| MS.gene054467 | MS.gene90179 | 0.803145 | 4.01E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene90179 | 0.816806 | 4.57E-52 | -1.69E-46 |
| MS.gene054559 | MS.gene90179 | 0.807206 | 5.66E-50 | -1.69E-46 |
| MS.gene055357 | MS.gene90179 | 0.804928 | 1.71E-49 | -1.69E-46 |
| MS.gene05604 | MS.gene90179 | 0.806367 | 8.51E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene90179 | 0.803457 | 3.45E-49 | -1.69E-46 |
| MS.gene056506 | MS.gene90179 | 0.801602 | 8.33E-49 | -1.69E-46 |
| MS.gene057026 | MS.gene90179 | 0.810749 | 9.87E-51 | -1.69E-46 |
| MS.gene057444 | MS.gene90179 | 0.823669 | 1.22E-53 | -1.69E-46 |
| MS.gene058625 | MS.gene90179 | 0.819464 | 1.15E-52 | -1.69E-46 |
| MS.gene058829 | MS.gene90179 | 0.813847 | 2.08E-51 | -1.69E-46 |
| MS.gene058979 | MS.gene90179 | 0.801045 | 1.08E-48 | -1.69E-46 |
| MS.gene060322 | MS.gene90179 | 0.805585 | 1.24E-49 | -1.69E-46 |
| MS.gene060521 | MS.gene90179 | 0.800605 | 1.33E-48 | -1.69E-46 |
| MS.gene06052 | MS.gene90179 | 0.802278 | 6.05E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene90179.t1 | MTR_3g111870 | 71.429 | 42 | 4 | 1 | 1 | 34 | 53 | 94 | 1.03e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 13 sgRNAs with CRISPR-Local
Find 30 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATAGGACAAACCTTGAT+AGG | 0.418182 | 4.2:+19328635 | None:intergenic |
| CGATTTGGAATATCCTATCA+AGG | 0.436524 | 4.2:-19328648 | MS.gene90179:intron |
| TCATCATGAGGCTGGAATGA+TGG | 0.453701 | 4.2:-19328341 | MS.gene90179:CDS |
| CAGAAATTCATGAGTACAGC+TGG | 0.484901 | 4.2:-19328400 | MS.gene90179:CDS |
| ATTCCAGCCTCATGATGAAA+AGG | 0.488849 | 4.2:+19328346 | None:intergenic |
| ACAGCTGGTGGTGGTGGTGA+TGG | 0.514789 | 4.2:-19328385 | MS.gene90179:CDS |
| ATGAGTACAGCTGGTGGTGG+TGG | 0.547213 | 4.2:-19328391 | MS.gene90179:CDS |
| ATTTGACATGGACTATACTC+TGG | 0.566549 | 4.2:-19328711 | MS.gene90179:CDS |
| GGTGGTGGTGATGGAATGAA+AGG | 0.569990 | 4.2:-19328376 | MS.gene90179:CDS |
| GGTGGTGATGGAATGAAAGG+TGG | 0.612810 | 4.2:-19328373 | MS.gene90179:CDS |
| TTCATGAGTACAGCTGGTGG+TGG | 0.613825 | 4.2:-19328394 | MS.gene90179:CDS |
| CTGGCACAATACAAGCCTGA+AGG | 0.646454 | 4.2:-19328692 | MS.gene90179:CDS |
| AAATTCATGAGTACAGCTGG+TGG | 0.700963 | 4.2:-19328397 | MS.gene90179:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TCATAATTACTGCAAGAAAA+AGG | + | chr4.2:19328484-19328503 | None:intergenic | 25.0% |
| ! | TCTTACATTCTGTTATACTT+TGG | - | chr4.2:19328615-19328634 | MS.gene90179:intron | 25.0% |
| ! | TTTCTTGCAGTAATTATGAT+GGG | - | chr4.2:19328485-19328504 | MS.gene90179:intron | 25.0% |
| !! | TTTTCTTGCAGTAATTATGA+TGG | - | chr4.2:19328484-19328503 | MS.gene90179:intron | 25.0% |
| TTCTTGCAGTAATTATGATG+GGG | - | chr4.2:19328486-19328505 | MS.gene90179:intron | 30.0% | |
| !! | TTTTGTTGTGGAATTTGACA+TGG | - | chr4.2:19328344-19328363 | MS.gene90179:CDS | 30.0% |
| CTCTTGGTTCACTATGTTAA+AGG | - | chr4.2:19328562-19328581 | MS.gene90179:intron | 35.0% | |
| TGCAAGAAAAAGGAGAAACA+TGG | + | chr4.2:19328474-19328493 | None:intergenic | 35.0% | |
| ! | ATTTGACATGGACTATACTC+TGG | - | chr4.2:19328356-19328375 | MS.gene90179:CDS | 35.0% |
| ! | CGATTTGGAATATCCTATCA+AGG | - | chr4.2:19328419-19328438 | MS.gene90179:intron | 35.0% |
| ! | TAAAAAGCTGGTCTACGATT+TGG | - | chr4.2:19328404-19328423 | MS.gene90179:intron | 35.0% |
| ! | TGAAGGCACAATTAAAAAGC+TGG | - | chr4.2:19328392-19328411 | MS.gene90179:CDS | 35.0% |
| !! | AGCTTTTTAATTGTGCCTTC+AGG | + | chr4.2:19328393-19328412 | None:intergenic | 35.0% |
| AAATTCATGAGTACAGCTGG+TGG | - | chr4.2:19328670-19328689 | MS.gene90179:CDS | 40.0% | |
| ATTCCAGCCTCATGATGAAA+AGG | + | chr4.2:19328724-19328743 | None:intergenic | 40.0% | |
| CAGAAATTCATGAGTACAGC+TGG | - | chr4.2:19328667-19328686 | MS.gene90179:CDS | 40.0% | |
| CAGATAGGACAAACCTTGAT+AGG | + | chr4.2:19328435-19328454 | None:intergenic | 40.0% | |
| GTTGCAGTAGTGTAGAGTTA+AGG | + | chr4.2:19328648-19328667 | None:intergenic | 40.0% | |
| TTTACTCACTCACTCACTCT+TGG | - | chr4.2:19328546-19328565 | MS.gene90179:intron | 40.0% | |
| ! | TGCAGTAATTATGATGGGGT+TGG | - | chr4.2:19328490-19328509 | MS.gene90179:intron | 40.0% |
| ! | TGGTGATCCTTTTCATCATG+AGG | - | chr4.2:19328714-19328733 | MS.gene90179:CDS | 40.0% |
| TCATCATGAGGCTGGAATGA+TGG | - | chr4.2:19328726-19328745 | MS.gene90179:CDS | 45.0% | |
| ! | GATCCTTTTCATCATGAGGC+TGG | - | chr4.2:19328718-19328737 | MS.gene90179:CDS | 45.0% |
| !! | GAGAGTGTTGCAAGACAGAT+AGG | + | chr4.2:19328450-19328469 | None:intergenic | 45.0% |
| CTGGCACAATACAAGCCTGA+AGG | - | chr4.2:19328375-19328394 | MS.gene90179:CDS | 50.0% | |
| TTCATGAGTACAGCTGGTGG+TGG | - | chr4.2:19328673-19328692 | MS.gene90179:CDS | 50.0% | |
| !! | GGTGGTGATGGAATGAAAGG+TGG | - | chr4.2:19328694-19328713 | MS.gene90179:CDS | 50.0% |
| !! | GGTGGTGGTGATGGAATGAA+AGG | - | chr4.2:19328691-19328710 | MS.gene90179:CDS | 50.0% |
| !! | ATGAGTACAGCTGGTGGTGG+TGG | - | chr4.2:19328676-19328695 | MS.gene90179:CDS | 55.0% |
| !! | ACAGCTGGTGGTGGTGGTGA+TGG | - | chr4.2:19328682-19328701 | MS.gene90179:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 19328333 | 19328756 | 19328333 | ID=MS.gene90179 |
| chr4.2 | mRNA | 19328333 | 19328756 | 19328333 | ID=MS.gene90179.t1;Parent=MS.gene90179 |
| chr4.2 | exon | 19328649 | 19328756 | 19328649 | ID=MS.gene90179.t1.exon1;Parent=MS.gene90179.t1 |
| chr4.2 | CDS | 19328649 | 19328756 | 19328649 | ID=cds.MS.gene90179.t1;Parent=MS.gene90179.t1 |
| chr4.2 | exon | 19328333 | 19328419 | 19328333 | ID=MS.gene90179.t1.exon2;Parent=MS.gene90179.t1 |
| chr4.2 | CDS | 19328333 | 19328419 | 19328333 | ID=cds.MS.gene90179.t1;Parent=MS.gene90179.t1 |
| Gene Sequence |
| Protein sequence |
