AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene90378

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90378.t1 MTR_2g105390 84.921 378 22 7 7 368 3 361 0.0 612
MS.gene90378.t1 MTR_1g022370 27.698 278 135 13 145 366 154 421 1.52e-13 72.0
MS.gene90378.t1 MTR_2g020280 53.226 62 22 2 312 366 232 293 1.05e-12 68.2
MS.gene90378.t1 MTR_2g020280 40.000 100 48 4 274 366 298 392 1.50e-12 68.6
MS.gene90378.t1 MTR_1g088960 23.934 305 153 11 129 366 109 401 2.25e-12 68.2
MS.gene90378.t1 MTR_2g007930 40.909 110 44 5 272 366 323 426 5.06e-12 67.4
MS.gene90378.t1 MTR_5g020580 38.947 95 44 3 281 366 326 415 9.48e-12 66.2
MS.gene90378.t1 MTR_8g077195 36.697 109 54 4 267 366 309 411 2.23e-11 65.1
MS.gene90378.t1 MTR_1g098790 36.207 116 47 4 272 366 321 430 2.91e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene90378.t1 AT1G16070 56.269 327 128 6 54 368 74 397 2.88e-125 366
MS.gene90378.t1 AT1G16070 54.122 279 113 6 54 320 74 349 1.24e-95 290
MS.gene90378.t1 AT1G16070 53.182 220 88 6 54 261 74 290 1.48e-70 223
MS.gene90378.t1 AT2G18280 25.948 343 177 13 91 366 60 392 3.74e-16 79.7
MS.gene90378.t1 AT2G18280 25.948 343 177 13 91 366 60 392 3.74e-16 79.7
MS.gene90378.t1 AT1G47270 26.953 256 136 10 154 366 140 387 2.26e-12 68.2
MS.gene90378.t1 AT1G47270 26.953 256 136 10 154 366 165 412 2.49e-12 68.2
MS.gene90378.t1 AT1G47270 26.953 256 136 10 154 366 164 411 2.52e-12 68.2
MS.gene90378.t1 AT2G47900 53.226 62 22 2 312 366 344 405 2.66e-12 68.2
MS.gene90378.t1 AT2G47900 53.226 62 22 2 312 366 344 405 2.66e-12 68.2
MS.gene90378.t1 AT2G47900 53.226 62 22 2 312 366 345 406 2.70e-12 68.2
MS.gene90378.t1 AT1G25280 32.090 134 82 3 240 366 135 266 3.48e-12 66.6
MS.gene90378.t1 AT1G25280 32.090 134 82 3 240 366 135 266 3.48e-12 66.6
MS.gene90378.t1 AT1G25280 31.884 138 69 3 249 366 312 444 1.20e-11 66.2
MS.gene90378.t1 AT3G06380 45.902 61 27 1 312 366 227 287 6.52e-11 63.2

Find 105 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCAAACTCTTTGAAGATT+TGG 0.208848 2.1:+608183 MS.gene90378:CDS
GTTGAAAATGAGTTGGATTT+TGG 0.248838 2.1:-608077 None:intergenic
GTTTACTACTGCGGGGCTTA+TGG 0.274791 2.1:-608672 None:intergenic
GGTTCAGGTACAACACATTA+AGG 0.279223 2.1:+608995 MS.gene90378:intron
TTCAAACTCTTTGAAGATTT+GGG 0.291430 2.1:+608184 MS.gene90378:CDS
AAACTTTCAGCTCACGTTAG+AGG 0.301321 2.1:+609263 MS.gene90378:CDS
CCTGTACATGTTGCTATTGT+AGG 0.305231 2.1:-608801 None:intergenic
GGTATAAAGAGAGAAGAAAT+TGG 0.306355 2.1:-608354 None:intergenic
CCGGTTCAAAATACCACATT+TGG 0.317816 2.1:+608632 MS.gene90378:CDS
TACGACCAAGGGTTTGGTTT+TGG 0.321955 2.1:-607953 None:intergenic
GAGCTTGACTATAGAGATAA+AGG 0.329596 2.1:+609207 MS.gene90378:CDS
AAAAGTATCATCATCAGAAT+TGG 0.355965 2.1:-608584 None:intergenic
AGACCATTGCCTATAGATAT+TGG 0.366407 2.1:+608278 MS.gene90378:CDS
TTAAGGGACTGCCAATGAAT+TGG 0.380340 2.1:+609012 MS.gene90378:CDS
GCAACAAAGCTTTGCATCAA+TGG 0.384089 2.1:-609589 None:intergenic
AAAATACCACATTTGGGATC+AGG 0.391655 2.1:+608639 MS.gene90378:CDS
TCCCACTCCGGCGCTTGTGG+TGG 0.398163 2.1:-608519 None:intergenic
GTGTACTTGTGTTATTGTTA+AGG 0.401096 2.1:+608304 MS.gene90378:CDS
ACCTCCCACTCCGGCGCTTG+TGG 0.404570 2.1:-608522 None:intergenic
AAAGACATAGATTGATGATT+GGG 0.409134 2.1:-608852 None:intergenic
AAAACATACGACCAAGGGTT+TGG 0.409386 2.1:-607959 None:intergenic
ATATCCTTTGACTGGTTACC+AGG 0.411071 2.1:+609545 MS.gene90378:CDS
TTCCTACCCTCCCTAGCTGC+AGG 0.422680 2.1:-609403 None:intergenic
CTGCTGCTCCTGCTTCCTCC+TGG 0.428096 2.1:+608225 MS.gene90378:CDS
TGTTAAGGAACCAACTCCTC+AGG 0.436248 2.1:+608319 MS.gene90378:CDS
GAAAGACATAGATTGATGAT+TGG 0.438091 2.1:-608853 None:intergenic
TCTACATATCAAAGACTTGT+TGG 0.438291 2.1:-608258 None:intergenic
GTACACCTTCCAATATCTAT+AGG 0.439094 2.1:-608287 None:intergenic
TAAGGGACTGCCAATGAATT+GGG 0.444518 2.1:+609013 MS.gene90378:CDS
GTTTGCTATGATTAGGAGAA+TGG 0.459646 2.1:+609368 MS.gene90378:intron
CGGTTCAAAATACCACATTT+GGG 0.463937 2.1:+608633 MS.gene90378:CDS
GATAAAGGAAGGCCAGGACT+TGG 0.466820 2.1:+609222 MS.gene90378:CDS
AAGAGGTGGTTTACTACTGC+GGG 0.474800 2.1:-608680 None:intergenic
GGGACGGCAGAATAGGAAAC+TGG 0.475585 2.1:+608489 MS.gene90378:CDS
AAGGTCAGGGACGGCAGAAT+AGG 0.479149 2.1:+608482 MS.gene90378:intron
CCCACTCCGGCGCTTGTGGT+GGG 0.479209 2.1:-608518 None:intergenic
GAAGAATCAGAAGAAGGAAT+AGG 0.486222 2.1:-608155 None:intergenic
CTTCAAAGAGTTTGAAAAGT+GGG 0.488487 2.1:-608177 None:intergenic
CTACATATCAAAGACTTGTT+GGG 0.489447 2.1:-608257 None:intergenic
GTATAAAGAGAGAAGAAATT+GGG 0.490055 2.1:-608353 None:intergenic
CGTGTTTGTTGAAAATGAGT+TGG 0.490098 2.1:-608084 None:intergenic
ATCTGCAGATATCCTTTGAC+TGG 0.494012 2.1:+609537 MS.gene90378:intron
CCAACTCCTCAGGGTCTCTC+CGG 0.498571 2.1:+608329 MS.gene90378:CDS
AAATACCACATTTGGGATCA+GGG 0.506568 2.1:+608640 MS.gene90378:CDS
CCGGAGAGACCCTGAGGAGT+TGG 0.514963 2.1:-608329 None:intergenic
AAAGTAGACCAGGAGGAAGC+AGG 0.520011 2.1:-608233 None:intergenic
AAGTGGGAAGAATCAGAAGA+AGG 0.520266 2.1:-608161 None:intergenic
AGCAGACAATCCTGCAGCTA+GGG 0.521878 2.1:+609393 MS.gene90378:CDS
GGAAGCCAAACATATGCAAA+AGG 0.524760 2.1:-609568 None:intergenic
CCTCCTACCTTGTTATAGAG+TGG 0.527819 2.1:-609077 None:intergenic
TATAGAGATAAAGGAAGGCC+AGG 0.527875 2.1:+609216 MS.gene90378:CDS
AAGCAGACAATCCTGCAGCT+AGG 0.533935 2.1:+609392 MS.gene90378:CDS
CTTGTTGGGTAAAGTAGACC+AGG 0.539180 2.1:-608243 None:intergenic
TTTGCTATGATTAGGAGAAT+GGG 0.539684 2.1:+609369 MS.gene90378:intron
CAAAACCAAAACCAAACCCT+TGG 0.542341 2.1:+607948 MS.gene90378:CDS
ATATAATACATTACATACAT+AGG 0.545980 2.1:-608375 None:intergenic
CTGTGGCCCACCACAAGCGC+CGG 0.547264 2.1:+608512 MS.gene90378:CDS
AAGTGCATCAACTATTCTCA+AGG 0.551216 2.1:+609051 MS.gene90378:CDS
GTTCAGGTACAACACATTAA+GGG 0.551230 2.1:+608996 MS.gene90378:intron
GATCTCTGGATTCCAAGTCC+TGG 0.553307 2.1:-609234 None:intergenic
AAGAGAGAAGAAATTGGGAC+CGG 0.559507 2.1:-608348 None:intergenic
AGACAATCCTGCAGCTAGGG+AGG 0.560928 2.1:+609396 MS.gene90378:CDS
GTCTTTCAAGAACACCAACC+AGG 0.561985 2.1:+608869 MS.gene90378:CDS
CCTACAATAGCAACATGTAC+AGG 0.562677 2.1:+608801 MS.gene90378:CDS
ACCACAAGCGCCGGAGTGGG+AGG 0.564951 2.1:+608521 MS.gene90378:CDS
CCCACCACAAGCGCCGGAGT+GGG 0.568859 2.1:+608518 MS.gene90378:CDS
TCTTCAAAGAGTTTGAAAAG+TGG 0.573900 2.1:-608178 None:intergenic
TTGCATGCAGAACTGTAATG+AGG 0.573968 2.1:-608123 None:intergenic
GACAATCCTGCAGCTAGGGA+GGG 0.574505 2.1:+609397 MS.gene90378:CDS
CCAAACATATGCAAAAGGCC+TGG 0.577415 2.1:-609563 None:intergenic
AGCTTTGTTGCATAGTGTAG+TGG 0.581324 2.1:+609601 MS.gene90378:CDS
GCCCACCACAAGCGCCGGAG+TGG 0.582189 2.1:+608517 MS.gene90378:CDS
ACAGTTACCGCTAACCTCAC+CGG 0.586784 2.1:+608613 MS.gene90378:CDS
AGGAGCAGCAGCTTCAGAGT+CGG 0.586966 2.1:-608213 None:intergenic
ATTTGAAAACATACGACCAA+GGG 0.596408 2.1:-607964 None:intergenic
GTTAAGGAACCAACTCCTCA+GGG 0.602181 2.1:+608320 MS.gene90378:CDS
CATTGCCTATAGATATTGGA+AGG 0.608050 2.1:+608282 MS.gene90378:CDS
TGAAGTGAGACCTCCCACTC+CGG 0.610091 2.1:-608531 None:intergenic
ACAGTAGCTCAGAATCTCAA+AGG 0.612108 2.1:+608553 MS.gene90378:CDS
AAGGCCTGGTAACCAGTCAA+AGG 0.614442 2.1:-609549 None:intergenic
TACTCAACAACAGCAAGAGG+TGG 0.614584 2.1:-608694 None:intergenic
GTTGGCATCAATCTCGCTGA+GGG 0.614601 2.1:-607988 None:intergenic
CTTCCAATATCTATAGGCAA+TGG 0.616470 2.1:-608281 None:intergenic
ATGAATCTAACTAACCTGGT+TGG 0.618468 2.1:-608883 None:intergenic
GCAGAATAGGAAACTGGCTG+TGG 0.618843 2.1:+608495 MS.gene90378:CDS
ATCCTGCAGCTAGGGAGGGT+AGG 0.625245 2.1:+609401 MS.gene90378:CDS
ATGCAGAACTGTAATGAGGA+AGG 0.628521 2.1:-608119 None:intergenic
TTGACTATAGAGATAAAGGA+AGG 0.630332 2.1:+609211 MS.gene90378:CDS
GCTTACTCAACAACAGCAAG+AGG 0.633778 2.1:-608697 None:intergenic
GGACTGCCAATGAATTGGGA+AGG 0.634626 2.1:+609017 MS.gene90378:CDS
CAAGAGGTGGTTTACTACTG+CGG 0.635758 2.1:-608681 None:intergenic
AGTGCATCAACTATTCTCAA+GGG 0.640841 2.1:+609052 MS.gene90378:CDS
GATATCCCTGATCCCAAATG+TGG 0.643558 2.1:-608645 None:intergenic
CAGCAGCTTCAGAGTCGGAG+TGG 0.644616 2.1:-608208 None:intergenic
TTGATGCCAACGTTCAGCTG+AGG 0.653807 2.1:+608000 MS.gene90378:CDS
TATTGTAGGAAGGTCAGGGA+CGG 0.654971 2.1:+608473 MS.gene90378:intron
TAGCTACAACAACAAGAACA+AGG 0.657649 2.1:+608031 MS.gene90378:CDS
ACTATGCCTCAGCTGAACGT+TGG 0.657801 2.1:-608006 None:intergenic
CGTTGGCATCAATCTCGCTG+AGG 0.662438 2.1:-607989 None:intergenic
GATTTGAAAACATACGACCA+AGG 0.673412 2.1:-607965 None:intergenic
AAGGGATCCACTCTATAACA+AGG 0.674607 2.1:+609070 MS.gene90378:CDS
AGAGGTGGTTTACTACTGCG+GGG 0.697959 2.1:-608679 None:intergenic
TTGGGACCGGAGAGACCCTG+AGG 0.711109 2.1:-608335 None:intergenic
TTGGCATCAATCTCGCTGAG+GGG 0.720118 2.1:-607987 None:intergenic
GTTGGGTAAAGTAGACCAGG+AGG 0.744497 2.1:-608240 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATATTATTATTATTATTGT+AGG + chr2.1:608459-608478 MS.gene90378:intron 10.0%
!! TATTACTAACAATAAAATTT+GGG + chr2.1:608973-608992 MS.gene90378:intron 10.0%
!! TTATTACTAACAATAAAATT+TGG + chr2.1:608972-608991 MS.gene90378:intron 10.0%
!! ATATAATACATTACATACAT+AGG - chr2.1:608378-608397 None:intergenic 15.0%
!! ATTACTAACAATAAAATTTG+GGG + chr2.1:608974-608993 MS.gene90378:intron 15.0%
!! TTAATGAAAATCAATATCAA+TGG - chr2.1:609148-609167 None:intergenic 15.0%
!! TTATTATTATTATTGTAGGA+AGG + chr2.1:608463-608482 MS.gene90378:intron 15.0%
!! ATACATAGAATAGAATAGAA+TGG - chr2.1:608410-608429 None:intergenic 20.0%
!!! AGGAAAATCTAATTTTGTTA+TGG + chr2.1:609421-609440 MS.gene90378:CDS 20.0%
!!! GTTTTTATGTTATAACAACT+CGG + chr2.1:609315-609334 MS.gene90378:intron 20.0%
! GTATAAAGAGAGAAGAAATT+GGG - chr2.1:608356-608375 None:intergenic 25.0%
! TTATAACATAAAAACCCTTC+CGG - chr2.1:609310-609329 None:intergenic 25.0%
!! AAAGACATAGATTGATGATT+GGG - chr2.1:608855-608874 None:intergenic 25.0%
!! TTCAAACTCTTTGAAGATTT+GGG + chr2.1:608184-608203 MS.gene90378:CDS 25.0%
!! TTTCAAACTCTTTGAAGATT+TGG + chr2.1:608183-608202 MS.gene90378:CDS 25.0%
!!! AAAAGTATCATCATCAGAAT+TGG - chr2.1:608587-608606 None:intergenic 25.0%
AATGAATTGGGAAGGAAAAA+TGG + chr2.1:609025-609044 MS.gene90378:CDS 30.0%
ATTATTATTGTAGGAAGGTC+AGG + chr2.1:608468-608487 MS.gene90378:intron 30.0%
ATTTGAAAACATACGACCAA+GGG - chr2.1:607967-607986 None:intergenic 30.0%
CTTCAAAGAGTTTGAAAAGT+GGG - chr2.1:608180-608199 None:intergenic 30.0%
GAAAATCAATATCAATGGCT+TGG - chr2.1:609143-609162 None:intergenic 30.0%
GAAAATGAATCTAACTAACC+TGG - chr2.1:608890-608909 None:intergenic 30.0%
GGTATAAAGAGAGAAGAAAT+TGG - chr2.1:608357-608376 None:intergenic 30.0%
TATAACATAAAAACCCTTCC+GGG - chr2.1:609309-609328 None:intergenic 30.0%
TCTACATATCAAAGACTTGT+TGG - chr2.1:608261-608280 None:intergenic 30.0%
TCTTCAAAGAGTTTGAAAAG+TGG - chr2.1:608181-608200 None:intergenic 30.0%
TTATTATTGTAGGAAGGTCA+GGG + chr2.1:608469-608488 MS.gene90378:intron 30.0%
TTGACTATAGAGATAAAGGA+AGG + chr2.1:609211-609230 MS.gene90378:CDS 30.0%
! AACAATAAAATTTGGGGTTC+AGG + chr2.1:608980-608999 MS.gene90378:intron 30.0%
! ATTTTTCCTTCCCAATTCAT+TGG - chr2.1:609026-609045 None:intergenic 30.0%
! CTACATATCAAAGACTTGTT+GGG - chr2.1:608260-608279 None:intergenic 30.0%
! GAAAGACATAGATTGATGAT+TGG - chr2.1:608856-608875 None:intergenic 30.0%
! GTGTACTTGTGTTATTGTTA+AGG + chr2.1:608304-608323 MS.gene90378:CDS 30.0%
! TAACTAGTACATCATTTTGC+AGG + chr2.1:608772-608791 MS.gene90378:intron 30.0%
! TTTGCTATGATTAGGAGAAT+GGG + chr2.1:609369-609388 MS.gene90378:intron 30.0%
!! CTAATTTTGTTATGGACTAC+AGG + chr2.1:609429-609448 MS.gene90378:CDS 30.0%
!! GATTTGTGTTTGCTATGATT+AGG + chr2.1:609361-609380 MS.gene90378:intron 30.0%
!! GTTGAAAATGAGTTGGATTT+TGG - chr2.1:608080-608099 None:intergenic 30.0%
!! TCTGATGATGATACTTTTCT+CGG + chr2.1:608589-608608 MS.gene90378:CDS 30.0%
!! TTTTGGAAGAGAAACAACAT+TGG - chr2.1:608063-608082 None:intergenic 30.0%
!!! TTTGGTTTTGGTTTTGGTTT+TGG - chr2.1:607938-607957 None:intergenic 30.0%
AAGTGCATCAACTATTCTCA+AGG + chr2.1:609051-609070 MS.gene90378:CDS 35.0%
AGACCATTGCCTATAGATAT+TGG + chr2.1:608278-608297 MS.gene90378:CDS 35.0%
AGTGCATCAACTATTCTCAA+GGG + chr2.1:609052-609071 MS.gene90378:CDS 35.0%
ATGAATCTAACTAACCTGGT+TGG - chr2.1:608886-608905 None:intergenic 35.0%
CATTGCCTATAGATATTGGA+AGG + chr2.1:608282-608301 MS.gene90378:CDS 35.0%
CGGTTCAAAATACCACATTT+GGG + chr2.1:608633-608652 MS.gene90378:CDS 35.0%
CTTAATGTGTATTGAAGAGG+CGG + chr2.1:609483-609502 MS.gene90378:intron 35.0%
CTTCCAATATCTATAGGCAA+TGG - chr2.1:608284-608303 None:intergenic 35.0%
GAAGAATCAGAAGAAGGAAT+AGG - chr2.1:608158-608177 None:intergenic 35.0%
GACCTTAATGTGTATTGAAG+AGG + chr2.1:609480-609499 MS.gene90378:intron 35.0%
GAGCTTGACTATAGAGATAA+AGG + chr2.1:609207-609226 MS.gene90378:CDS 35.0%
GATTTGAAAACATACGACCA+AGG - chr2.1:607968-607987 None:intergenic 35.0%
GTACACCTTCCAATATCTAT+AGG - chr2.1:608290-608309 None:intergenic 35.0%
GTTCAGGTACAACACATTAA+GGG + chr2.1:608996-609015 MS.gene90378:intron 35.0%
TAGCTACAACAACAAGAACA+AGG + chr2.1:608031-608050 MS.gene90378:CDS 35.0%
TTAATGTGTATTGAAGAGGC+GGG + chr2.1:609484-609503 MS.gene90378:intron 35.0%
TTAGTTAGCAGAGAAGAATG+AGG - chr2.1:608755-608774 None:intergenic 35.0%
TTGCTTTGTACTCTTGATGA+AGG - chr2.1:609184-609203 None:intergenic 35.0%
! AAAATACCACATTTGGGATC+AGG + chr2.1:608639-608658 MS.gene90378:CDS 35.0%
! AAATACCACATTTGGGATCA+GGG + chr2.1:608640-608659 MS.gene90378:CDS 35.0%
! ACTCATTTTCAACAAACACG+AGG + chr2.1:608087-608106 MS.gene90378:CDS 35.0%
! CGTGAGCTGAAAGTTTTTAA+CGG - chr2.1:609259-609278 None:intergenic 35.0%
! CGTGTTTGTTGAAAATGAGT+TGG - chr2.1:608087-608106 None:intergenic 35.0%
!! CCAAATGTGGTATTTTGAAC+CGG - chr2.1:608635-608654 None:intergenic 35.0%
!! GAAAGTTTTTAACGGATCTC+TGG - chr2.1:609251-609270 None:intergenic 35.0%
!! GTTTGCTATGATTAGGAGAA+TGG + chr2.1:609368-609387 MS.gene90378:intron 35.0%
AAAACATACGACCAAGGGTT+TGG - chr2.1:607962-607981 None:intergenic 40.0%
AAACTTTCAGCTCACGTTAG+AGG + chr2.1:609263-609282 MS.gene90378:CDS 40.0%
AAAGAAACTTCCCCTAAGTG+AGG - chr2.1:609105-609124 None:intergenic 40.0%
AAGAGAGAAGAAATTGGGAC+CGG - chr2.1:608351-608370 None:intergenic 40.0%
AAGGGATCCACTCTATAACA+AGG + chr2.1:609070-609089 MS.gene90378:CDS 40.0%
AAGTGGGAAGAATCAGAAGA+AGG - chr2.1:608164-608183 None:intergenic 40.0%
ACAGTAGCTCAGAATCTCAA+AGG + chr2.1:608553-608572 MS.gene90378:CDS 40.0%
AGGTGGAGATTGAGAGATTT+AGG - chr2.1:608722-608741 None:intergenic 40.0%
ATCTGCAGATATCCTTTGAC+TGG + chr2.1:609537-609556 MS.gene90378:intron 40.0%
ATGCAGAACTGTAATGAGGA+AGG - chr2.1:608122-608141 None:intergenic 40.0%
ATTGACAGAGGAAATGGTAC+TGG - chr2.1:609519-609538 None:intergenic 40.0%
CAAAACCAAAACCAAACCCT+TGG + chr2.1:607948-607967 MS.gene90378:CDS 40.0%
CCGGTTCAAAATACCACATT+TGG + chr2.1:608632-608651 MS.gene90378:CDS 40.0%
CCTACAATAGCAACATGTAC+AGG + chr2.1:608801-608820 MS.gene90378:CDS 40.0%
CGCCTCTTCAATACACATTA+AGG - chr2.1:609485-609504 None:intergenic 40.0%
GATCCACTCTATAACAAGGT+AGG + chr2.1:609074-609093 MS.gene90378:intron 40.0%
GCAACAAAGCTTTGCATCAA+TGG - chr2.1:609592-609611 None:intergenic 40.0%
GGAAGCCAAACATATGCAAA+AGG - chr2.1:609571-609590 None:intergenic 40.0%
GGTTCAGGTACAACACATTA+AGG + chr2.1:608995-609014 MS.gene90378:intron 40.0%
TAAATCTCTCAATCTCCACC+TGG + chr2.1:608721-608740 MS.gene90378:intron 40.0%
TAAGGGACTGCCAATGAATT+GGG + chr2.1:609013-609032 MS.gene90378:CDS 40.0%
TAATGTGTATTGAAGAGGCG+GGG + chr2.1:609485-609504 MS.gene90378:intron 40.0%
TATAGAGATAAAGGAAGGCC+AGG + chr2.1:609216-609235 MS.gene90378:CDS 40.0%
TTAAGGGACTGCCAATGAAT+TGG + chr2.1:609012-609031 MS.gene90378:CDS 40.0%
TTGCATGCAGAACTGTAATG+AGG - chr2.1:608126-608145 None:intergenic 40.0%
! ATATCCTTTGACTGGTTACC+AGG + chr2.1:609545-609564 MS.gene90378:CDS 40.0%
! CCTGTACATGTTGCTATTGT+AGG - chr2.1:608804-608823 None:intergenic 40.0%
!!! CAAGGGTTTGGTTTTGGTTT+TGG - chr2.1:607950-607969 None:intergenic 40.0%
AAGAGGTGGTTTACTACTGC+GGG - chr2.1:608683-608702 None:intergenic 45.0%
AGAGGTAACATCATTGACGC+CGG + chr2.1:609281-609300 MS.gene90378:intron 45.0%
ATAAAAACCCTTCCGGGTTC+CGG - chr2.1:609303-609322 None:intergenic 45.0%
CAAGAGGTGGTTTACTACTG+CGG - chr2.1:608684-608703 None:intergenic 45.0%
CCAAACATATGCAAAAGGCC+TGG - chr2.1:609566-609585 None:intergenic 45.0%
CCACTCTATAACAAGGTAGG+AGG + chr2.1:609077-609096 MS.gene90378:intron 45.0%
CTGCAGATTGACAGAGGAAA+TGG - chr2.1:609525-609544 None:intergenic 45.0%
CTTGTTGGGTAAAGTAGACC+AGG - chr2.1:608246-608265 None:intergenic 45.0%
GATATCCCTGATCCCAAATG+TGG - chr2.1:608648-608667 None:intergenic 45.0%
GCTTACTCAACAACAGCAAG+AGG - chr2.1:608700-608719 None:intergenic 45.0%
GGATATCTGCAGATTGACAG+AGG - chr2.1:609531-609550 None:intergenic 45.0%
GTCTTTCAAGAACACCAACC+AGG + chr2.1:608869-608888 MS.gene90378:CDS 45.0%
GTTAAGGAACCAACTCCTCA+GGG + chr2.1:608320-608339 MS.gene90378:CDS 45.0%
GTTAGCAGAGAAGAATGAGG+CGG - chr2.1:608752-608771 None:intergenic 45.0%
TACTCAACAACAGCAAGAGG+TGG - chr2.1:608697-608716 None:intergenic 45.0%
TATTGTAGGAAGGTCAGGGA+CGG + chr2.1:608473-608492 MS.gene90378:intron 45.0%
TGTTAAGGAACCAACTCCTC+AGG + chr2.1:608319-608338 MS.gene90378:CDS 45.0%
! CCAGGCCTTTTGCATATGTT+TGG + chr2.1:609563-609582 MS.gene90378:CDS 45.0%
! CCTCCTACCTTGTTATAGAG+TGG - chr2.1:609080-609099 None:intergenic 45.0%
! TTTTGAACCGGTGAGGTTAG+CGG - chr2.1:608623-608642 None:intergenic 45.0%
!! TACGACCAAGGGTTTGGTTT+TGG - chr2.1:607956-607975 None:intergenic 45.0%
!! TGTGGTATTTTGAACCGGTG+AGG - chr2.1:608630-608649 None:intergenic 45.0%
AAAGTAGACCAGGAGGAAGC+AGG - chr2.1:608236-608255 None:intergenic 50.0%
AAGCAGACAATCCTGCAGCT+AGG + chr2.1:609392-609411 MS.gene90378:CDS 50.0%
AAGGCCTGGTAACCAGTCAA+AGG - chr2.1:609552-609571 None:intergenic 50.0%
ACAGTTACCGCTAACCTCAC+CGG + chr2.1:608613-608632 MS.gene90378:CDS 50.0%
ACTATGCCTCAGCTGAACGT+TGG - chr2.1:608009-608028 None:intergenic 50.0%
AGAGGTGGTTTACTACTGCG+GGG - chr2.1:608682-608701 None:intergenic 50.0%
AGCAGACAATCCTGCAGCTA+GGG + chr2.1:609393-609412 MS.gene90378:CDS 50.0%
GATAAAGGAAGGCCAGGACT+TGG + chr2.1:609222-609241 MS.gene90378:CDS 50.0%
GATCTCTGGATTCCAAGTCC+TGG - chr2.1:609237-609256 None:intergenic 50.0%
GCAGAATAGGAAACTGGCTG+TGG + chr2.1:608495-608514 MS.gene90378:CDS 50.0%
GGACTGCCAATGAATTGGGA+AGG + chr2.1:609017-609036 MS.gene90378:CDS 50.0%
GTTGGCATCAATCTCGCTGA+GGG - chr2.1:607991-608010 None:intergenic 50.0%
GTTGGGTAAAGTAGACCAGG+AGG - chr2.1:608243-608262 None:intergenic 50.0%
GTTTACTACTGCGGGGCTTA+TGG - chr2.1:608675-608694 None:intergenic 50.0%
TTGATGCCAACGTTCAGCTG+AGG + chr2.1:608000-608019 MS.gene90378:CDS 50.0%
TTGGCATCAATCTCGCTGAG+GGG - chr2.1:607990-608009 None:intergenic 50.0%
AAGGTCAGGGACGGCAGAAT+AGG + chr2.1:608482-608501 MS.gene90378:intron 55.0%
ACATCATTGACGCCGGAACC+CGG + chr2.1:609288-609307 MS.gene90378:intron 55.0%
AGACAATCCTGCAGCTAGGG+AGG + chr2.1:609396-609415 MS.gene90378:CDS 55.0%
AGGAGCAGCAGCTTCAGAGT+CGG - chr2.1:608216-608235 None:intergenic 55.0%
AGGTAGGAGGAGCCTCACTT+AGG + chr2.1:609090-609109 MS.gene90378:intron 55.0%
CGTTGGCATCAATCTCGCTG+AGG - chr2.1:607992-608011 None:intergenic 55.0%
GACAATCCTGCAGCTAGGGA+GGG + chr2.1:609397-609416 MS.gene90378:CDS 55.0%
GGGACGGCAGAATAGGAAAC+TGG + chr2.1:608489-608508 MS.gene90378:CDS 55.0%
GGTAGGAGGAGCCTCACTTA+GGG + chr2.1:609091-609110 MS.gene90378:intron 55.0%
GTAGGAGGAGCCTCACTTAG+GGG + chr2.1:609092-609111 MS.gene90378:intron 55.0%
TGAAGTGAGACCTCCCACTC+CGG - chr2.1:608534-608553 None:intergenic 55.0%
ATCCTGCAGCTAGGGAGGGT+AGG + chr2.1:609401-609420 MS.gene90378:CDS 60.0%
ATTGACGCCGGAACCCGGAA+GGG + chr2.1:609293-609312 MS.gene90378:intron 60.0%
CAGAGAAGAATGAGGCGGCC+AGG - chr2.1:608747-608766 None:intergenic 60.0%
CAGCAGCTTCAGAGTCGGAG+TGG - chr2.1:608211-608230 None:intergenic 60.0%
CCAACTCCTCAGGGTCTCTC+CGG + chr2.1:608329-608348 MS.gene90378:CDS 60.0%
CTCTCAATCTCCACCTGGCC+TGG + chr2.1:608726-608745 MS.gene90378:intron 60.0%
TTCCTACCCTCCCTAGCTGC+AGG - chr2.1:609406-609425 None:intergenic 60.0%
AAGAATGAGGCGGCCAGGCC+AGG - chr2.1:608742-608761 None:intergenic 65.0%
CATTGACGCCGGAACCCGGA+AGG + chr2.1:609292-609311 MS.gene90378:intron 65.0%
CCGGAGAGACCCTGAGGAGT+TGG - chr2.1:608332-608351 None:intergenic 65.0%
CTGCTGCTCCTGCTTCCTCC+TGG + chr2.1:608225-608244 MS.gene90378:CDS 65.0%
TTGGGACCGGAGAGACCCTG+AGG - chr2.1:608338-608357 None:intergenic 65.0%
AATGAGGCGGCCAGGCCAGG+TGG - chr2.1:608739-608758 None:intergenic 70.0%
ACCTCCCACTCCGGCGCTTG+TGG - chr2.1:608525-608544 None:intergenic 70.0%
CCCACTCCGGCGCTTGTGGT+GGG - chr2.1:608521-608540 None:intergenic 70.0%
CTGTGGCCCACCACAAGCGC+CGG + chr2.1:608512-608531 MS.gene90378:CDS 70.0%
TCCCACTCCGGCGCTTGTGG+TGG - chr2.1:608522-608541 None:intergenic 70.0%
!! ACCACAAGCGCCGGAGTGGG+AGG + chr2.1:608521-608540 MS.gene90378:CDS 70.0%
!! CCCACCACAAGCGCCGGAGT+GGG + chr2.1:608518-608537 MS.gene90378:CDS 70.0%
!! GCCCACCACAAGCGCCGGAG+TGG + chr2.1:608517-608536 MS.gene90378:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 607930 609620 607930 ID=MS.gene90378
chr2.1 mRNA 607930 609620 607930 ID=MS.gene90378.t1;Parent=MS.gene90378
chr2.1 exon 607930 608383 607930 ID=MS.gene90378.t1.exon1;Parent=MS.gene90378.t1
chr2.1 CDS 607930 608383 607930 ID=cds.MS.gene90378.t1;Parent=MS.gene90378.t1
chr2.1 exon 608485 608713 608485 ID=MS.gene90378.t1.exon2;Parent=MS.gene90378.t1
chr2.1 CDS 608485 608713 608485 ID=cds.MS.gene90378.t1;Parent=MS.gene90378.t1
chr2.1 exon 608794 608890 608794 ID=MS.gene90378.t1.exon3;Parent=MS.gene90378.t1
chr2.1 CDS 608794 608890 608794 ID=cds.MS.gene90378.t1;Parent=MS.gene90378.t1
chr2.1 exon 609002 609091 609002 ID=MS.gene90378.t1.exon4;Parent=MS.gene90378.t1
chr2.1 CDS 609002 609091 609002 ID=cds.MS.gene90378.t1;Parent=MS.gene90378.t1
chr2.1 exon 609192 609284 609192 ID=MS.gene90378.t1.exon5;Parent=MS.gene90378.t1
chr2.1 CDS 609192 609284 609192 ID=cds.MS.gene90378.t1;Parent=MS.gene90378.t1
chr2.1 exon 609383 609450 609383 ID=MS.gene90378.t1.exon6;Parent=MS.gene90378.t1
chr2.1 CDS 609383 609450 609383 ID=cds.MS.gene90378.t1;Parent=MS.gene90378.t1
chr2.1 exon 609545 609620 609545 ID=MS.gene90378.t1.exon7;Parent=MS.gene90378.t1
chr2.1 CDS 609545 609620 609545 ID=cds.MS.gene90378.t1;Parent=MS.gene90378.t1
Gene Sequence

>MS.gene90378

ATGAAGAAAAACCAAAACCAAAACCAAAACCAAACCCTTGGTCGTATGTTTTCAAATCCCCTCAGCGAGATTGATGCCAACGTTCAGCTGAGGCATAGTCGTAGCTACAACAACAAGAACAAGGAAAATGCCAATGTTGTTTCTCTTCCAAAATCCAACTCATTTTCAACAAACACGAGGATTCTTAAGCCTTCCTCATTACAGTTCTGCATGCAAATAAACGATCCTATTCCTTCTTCTGATTCTTCCCACTTTTCAAACTCTTTGAAGATTTGGGACCACTCCGACTCTGAAGCTGCTGCTCCTGCTTCCTCCTGGTCTACTTTACCCAACAAGTCTTTGATATGTAGACCATTGCCTATAGATATTGGAAGGTGTACTTGTGTTATTGTTAAGGAACCAACTCCTCAGGGTCTCTCCGGTCCCAATTTCTTCTCTCTTTATACCTATGTATGTAATGTATTATATTATTCACTACCATTCTATTCTATTCTATGTATAATATAATATCACTTTGCTATTTAATCAACATATTATTATTATTATTGTAGGAAGGTCAGGGACGGCAGAATAGGAAACTGGCTGTGGCCCACCACAAGCGCCGGAGTGGGAGGTCTCACTTCACAGTAGCTCAGAATCTCAAAGGACTACTCTCCAATTCTGATGATGATACTTTTCTCGGTACAGTTACCGCTAACCTCACCGGTTCAAAATACCACATTTGGGATCAGGGATATCATCGCCATAAGCCCCGCAGTAGTAAACCACCTCTTGCTGTTGTTGAGTAAGCCTAAATCTCTCAATCTCCACCTGGCCTGGCCGCCTCATTCTTCTCTGCTAACTAACTAGTACATCATTTTGCAGGTATGTACCTACAATAGCAACATGTACAGGAACTCACAGAAGCATCAAAGCTTATATACCCAATCATCAATCTATGTCTTTCAAGAACACCAACCAGGTTAGTTAGATTCATTTTCAATAATATCATGAATCATGATCACATCTACTGTGTCGTCAGTTCAATTACAACAATATCTCTTTATTACTAACAATAAAATTTGGGGTTCAGGTACAACACATTAAGGGACTGCCAATGAATTGGGAAGGAAAAATGGACAAAGTGCATCAACTATTCTCAAGGGATCCACTCTATAACAAGGTAGGAGGAGCCTCACTTAGGGGAAGTTTCTTTGTCATTTATAGCTAGCCAAGCCATTGATATTGATTTTCATTAATTTGTATTGTCTTCCTTCATCAAGAGTACAAAGCAATTTGAGCTTGACTATAGAGATAAAGGAAGGCCAGGACTTGGAATCCAGAGATCCGTTAAAAACTTTCAGCTCACGTTAGAGGTAACATCATTGACGCCGGAACCCGGAAGGGTTTTTATGTTATAACAACTCGGTTAGTTATGTTGTCTACTTAGCTGATTTGTGTTTGCTATGATTAGGAGAATGGGAAGCAGACAATCCTGCAGCTAGGGAGGGTAGGAAAATCTAATTTTGTTATGGACTACAGGTATCAATTCAAATTTTTCTTGTTTTATTGACCTTAATGTGTATTGAAGAGGCGGGGTTTCATAGCCAGTACCATTTCCTCTGTCAATCTGCAGATATCCTTTGACTGGTTACCAGGCCTTTTGCATATGTTTGGCTTCCATTGATGCAAAGCTTTGTTGCATAGTGTAG
Protein sequence

>MS.gene90378.t1

MKKNQNQNQNQTLGRMFSNPLSEIDANVQLRHSRSYNNKNKENANVVSLPKSNSFSTNTRILKPSSLQFCMQINDPIPSSDSSHFSNSLKIWDHSDSEAAAPASSWSTLPNKSLICRPLPIDIGRCTCVIVKEPTPQGLSGPNFFSLYTYVCQGRQNRKLAVAHHKRRSGRSHFTVAQNLKGLLSNSDDDTFLGTVTANLTGSKYHIWDQGYHRHKPRSSKPPLAVVEYVPTIATCTGTHRSIKAYIPNHQSMSFKNTNQVQHIKGLPMNWEGKMDKVHQLFSRDPLYNKSTKQFELDYRDKGRPGLGIQRSVKNFQLTLEENGKQTILQLGRVGKSNFVMDYRYPLTGYQAFCICLASIDAKLCCIV