AlfalfaGEDB | MS.gene91652

M. sativa cultivar XinJiangDaYe / MS.gene91652

KEGG: K02894
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: PF00238.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91652.t1	XP_018810074.1	96	101	4	0	1	101	1	101	7.90E-48	199.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91652.t1	Q07760	92.1	101	8	0	1	101	1	101	3.6e-49	195.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91652.t1	A0A2I4DSF1	96.0	101	4	0	1	101	1	101	5.7e-48	199.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049821	MS.gene91652	0.809106	2.23E-50	-1.69E-46
MS.gene049825	MS.gene91652	0.811621	6.39E-51	-1.69E-46
MS.gene050303	MS.gene91652	0.807771	4.29E-50	-1.69E-46
MS.gene051700	MS.gene91652	0.82029	7.42E-53	-1.69E-46
MS.gene05188	MS.gene91652	0.80297	4.36E-49	-1.69E-46
MS.gene051975	MS.gene91652	0.805105	1.57E-49	-1.69E-46
MS.gene052947	MS.gene91652	0.823052	1.70E-53	-1.69E-46
MS.gene053294	MS.gene91652	0.814813	1.27E-51	-1.69E-46
MS.gene053931	MS.gene91652	0.801081	1.07E-48	-1.69E-46
MS.gene053963	MS.gene91652	0.820939	5.26E-53	-1.69E-46
MS.gene05548	MS.gene91652	0.821343	4.25E-53	-1.69E-46
MS.gene055555	MS.gene91652	0.802506	5.43E-49	-1.69E-46
MS.gene055808	MS.gene91652	0.834111	3.62E-56	-1.69E-46
MS.gene055841	MS.gene91652	0.808957	2.40E-50	-1.69E-46
MS.gene056091	MS.gene91652	0.810756	9.84E-51	-1.69E-46
MS.gene056215	MS.gene91652	0.835245	1.88E-56	-1.69E-46
MS.gene05689	MS.gene91652	0.824766	6.76E-54	-1.69E-46
MS.gene056986	MS.gene91652	0.810081	1.38E-50	-1.69E-46
MS.gene057705	MS.gene91652	0.825645	4.19E-54	-1.69E-46
MS.gene058074	MS.gene91652	0.806686	7.29E-50	-1.69E-46
MS.gene058159	MS.gene91652	0.804823	1.80E-49	-1.69E-46
MS.gene05954	MS.gene91652	0.815194	1.05E-51	-1.69E-46
MS.gene060429	MS.gene91652	0.82787	1.23E-54	-1.69E-46
MS.gene060917	MS.gene91652	0.817935	2.55E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91652.t1	MTR_7g098290	96.040	101	4	0	1	101	1	101	2.04e-66	196
MS.gene91652.t1	MTR_1g069585	96.040	101	4	0	1	101	1	101	2.04e-66	196
MS.gene91652.t1	MTR_7g098290	95.349	86	4	0	16	101	1	86	2.76e-55	168
MS.gene91652.t1	MTR_1g026090	73.000	100	12	1	2	101	42	126	2.10e-42	137
MS.gene91652.t1	MTR_5g464620	84.000	50	8	0	13	62	15	64	5.58e-24	87.4
MS.gene91652.t1	MTR_5g054560	54.651	86	15	1	16	101	1	62	5.33e-23	85.9

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene91652.t1	AT3G04400	93.069	101	7	1	101	1	101	8.43e-65	192
MS.gene91652.t1	AT2G33370	93.069	101	7	1	101	1	101	8.43e-65	192
MS.gene91652.t1	AT1G04480	93.069	101	7	1	101	1	101	8.43e-65	192
MS.gene91652.t1	AT3G04400	94.186	86	5	16	101	1	86	1.76e-54	166
MS.gene91652.t1	AT2G33370	94.186	86	5	16	101	1	86	1.76e-54	166

Find 35 sgRNAs with CRISPR-Local

Find 39 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAACAAGTTCCGTATGTCAC+TGG	0.413902	7.4:-85942213	MS.gene91652:CDS
ACATGAAGACACCGTCCTTT+CGG	0.413999	7.4:+85941979	None:intergenic
CCTACCCTTGATTCCCTTCA+CGG	0.419579	7.4:+85942124	None:intergenic
AAAACATTCTTCCTGAGATC+AGG	0.427356	7.4:+85942038	None:intergenic
CGTCTTCCTTCAGCTTGTGC+TGG	0.450748	7.4:-85942095	MS.gene91652:CDS
TTAGGTCGTGGTGGTTCCGC+GGG	0.471837	7.4:-85942236	MS.gene91652:intron
CATCTCCGTGAAGGGAATCA+AGG	0.491257	7.4:-85942129	MS.gene91652:CDS
GGTGTCTTCATGTACTTTGA+AGG	0.499615	7.4:-85941969	MS.gene91652:CDS
CAGTTCACCGTCGCTGCCAC+TGG	0.502403	7.4:+85942185	None:intergenic
TTGTGCTGGTGACGTGGTGA+TGG	0.519017	7.4:-85942081	MS.gene91652:CDS
AGAAGGGAAAGCCTGATCTC+AGG	0.525390	7.4:-85942049	MS.gene91652:CDS
TGCTGACGAACGATGACAGC+TGG	0.538722	7.4:+85942014	None:intergenic
ACACCGTCCTTTCGGCGCCA+TGG	0.546165	7.4:+85941987	None:intergenic
AACAAGTTCCGTATGTCACT+GGG	0.554811	7.4:-85942212	MS.gene91652:CDS
AAGCCATGGCGCCGAAAGGA+CGG	0.558400	7.4:-85941990	MS.gene91652:CDS
ATTAGGTCGTGGTGGTTCCG+CGG	0.570592	7.4:-85942237	MS.gene91652:intron
CTGGTAGACCCAGTGACATA+CGG	0.574663	7.4:+85942204	None:intergenic
GGTGATGGCTACTGTGAAGA+AGG	0.577572	7.4:-85942066	MS.gene91652:CDS
TTCAGCTTGTGCTGGTGACG+TGG	0.580455	7.4:-85942087	MS.gene91652:CDS
GGGTCTACCAGTGGCAGCGA+CGG	0.583652	7.4:-85942192	MS.gene91652:CDS
CTGTACATCATCTCCGTGAA+GGG	0.589573	7.4:-85942137	MS.gene91652:CDS
GATGTACAGATTCTTTGCTC+CGG	0.589715	7.4:+85942151	None:intergenic
ATCTCCGTGAAGGGAATCAA+GGG	0.592804	7.4:-85942128	MS.gene91652:CDS
TCTGTACATCATCTCCGTGA+AGG	0.600106	7.4:-85942138	MS.gene91652:CDS
GCATAAGCCATGGCGCCGAA+AGG	0.608624	7.4:-85941994	MS.gene91652:CDS
ACGTCACCAGCACAAGCTGA+AGG	0.618315	7.4:+85942089	None:intergenic
AACGCAACCATGTCGAAGAG+AGG	0.636545	7.4:-85942337	None:intergenic
TCGTTCGTCAGCATAAGCCA+TGG	0.638983	7.4:-85942004	MS.gene91652:CDS
CCGTGAAGGGAATCAAGGGT+AGG	0.651559	7.4:-85942124	MS.gene91652:CDS
TATGTCACTGGGTCTACCAG+TGG	0.667978	7.4:-85942201	MS.gene91652:CDS
GTGATGGCTACTGTGAAGAA+GGG	0.669945	7.4:-85942065	MS.gene91652:CDS
GTACATACCTCTCTTCGACA+TGG	0.681454	7.4:+85942330	None:intergenic
GTGAACTGTGCTGATAACAC+CGG	0.699857	7.4:-85942170	MS.gene91652:CDS
TAGGTCGTGGTGGTTCCGCG+GGG	0.708397	7.4:-85942235	MS.gene91652:CDS
CATACGGAACTTGTTCCCCG+CGG	0.790506	7.4:+85942220	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATACAATTGAATCTGAGAA+AGG	+	chr7.4:85941975-85941994	None:intergenic	25.0%
!	TGTATCCCTATTTTCTGATT+AGG	-	chr7.4:85942012-85942031	MS.gene91652:CDS	30.0%
	AAAACATTCTTCCTGAGATC+AGG	+	chr7.4:85942231-85942250	None:intergenic	35.0%
	CACGACCTAATCAGAAAATA+GGG	+	chr7.4:85942020-85942039	None:intergenic	35.0%
	AACAAGTTCCGTATGTCACT+GGG	-	chr7.4:85942054-85942073	MS.gene91652:CDS	40.0%
	CCACGACCTAATCAGAAAAT+AGG	+	chr7.4:85942021-85942040	None:intergenic	40.0%
	GATGTACAGATTCTTTGCTC+CGG	+	chr7.4:85942118-85942137	None:intergenic	40.0%
	GGTGTCTTCATGTACTTTGA+AGG	-	chr7.4:85942297-85942316	MS.gene91652:intron	40.0%
!	ATTTTCTGATTAGGTCGTGG+TGG	-	chr7.4:85942021-85942040	MS.gene91652:CDS	40.0%
!	CCTATTTTCTGATTAGGTCG+TGG	-	chr7.4:85942018-85942037	MS.gene91652:CDS	40.0%
	ACATGAAGACACCGTCCTTT+CGG	+	chr7.4:85942290-85942309	None:intergenic	45.0%
	ATCTCCGTGAAGGGAATCAA+GGG	-	chr7.4:85942138-85942157	MS.gene91652:CDS	45.0%
	CTGTACATCATCTCCGTGAA+GGG	-	chr7.4:85942129-85942148	MS.gene91652:CDS	45.0%
	GAACAAGTTCCGTATGTCAC+TGG	-	chr7.4:85942053-85942072	MS.gene91652:CDS	45.0%
	GTGATGGCTACTGTGAAGAA+GGG	-	chr7.4:85942201-85942220	MS.gene91652:CDS	45.0%
	TCTGTACATCATCTCCGTGA+AGG	-	chr7.4:85942128-85942147	MS.gene91652:CDS	45.0%
!	GTGAACTGTGCTGATAACAC+CGG	-	chr7.4:85942096-85942115	MS.gene91652:CDS	45.0%
	AGAAGGGAAAGCCTGATCTC+AGG	-	chr7.4:85942217-85942236	MS.gene91652:CDS	50.0%
	CATCTCCGTGAAGGGAATCA+AGG	-	chr7.4:85942137-85942156	MS.gene91652:CDS	50.0%
	CCTACCCTTGATTCCCTTCA+CGG	+	chr7.4:85942145-85942164	None:intergenic	50.0%
	CTGGTAGACCCAGTGACATA+CGG	+	chr7.4:85942065-85942084	None:intergenic	50.0%
	TATGTCACTGGGTCTACCAG+TGG	-	chr7.4:85942065-85942084	MS.gene91652:CDS	50.0%
	TCGTTCGTCAGCATAAGCCA+TGG	-	chr7.4:85942262-85942281	MS.gene91652:intron	50.0%
!	GGTGATGGCTACTGTGAAGA+AGG	-	chr7.4:85942200-85942219	MS.gene91652:CDS	50.0%
	ACGTCACCAGCACAAGCTGA+AGG	+	chr7.4:85942180-85942199	None:intergenic	55.0%
	ATTAGGTCGTGGTGGTTCCG+CGG	-	chr7.4:85942029-85942048	MS.gene91652:CDS	55.0%
	CCGTGAAGGGAATCAAGGGT+AGG	-	chr7.4:85942142-85942161	MS.gene91652:CDS	55.0%
	CGTCTTCCTTCAGCTTGTGC+TGG	-	chr7.4:85942171-85942190	MS.gene91652:CDS	55.0%
	TGCTGACGAACGATGACAGC+TGG	+	chr7.4:85942255-85942274	None:intergenic	55.0%
!	CATACGGAACTTGTTCCCCG+CGG	+	chr7.4:85942049-85942068	None:intergenic	55.0%
!	TTCAGCTTGTGCTGGTGACG+TGG	-	chr7.4:85942179-85942198	MS.gene91652:CDS	55.0%
!	TTGTGCTGGTGACGTGGTGA+TGG	-	chr7.4:85942185-85942204	MS.gene91652:CDS	55.0%
	TTAGGTCGTGGTGGTTCCGC+GGG	-	chr7.4:85942030-85942049	MS.gene91652:CDS	60.0%
!	AAGCCATGGCGCCGAAAGGA+CGG	-	chr7.4:85942276-85942295	MS.gene91652:intron	60.0%
!	GCATAAGCCATGGCGCCGAA+AGG	-	chr7.4:85942272-85942291	MS.gene91652:intron	60.0%
	ACACCGTCCTTTCGGCGCCA+TGG	+	chr7.4:85942282-85942301	None:intergenic	65.0%
	CAGTTCACCGTCGCTGCCAC+TGG	+	chr7.4:85942084-85942103	None:intergenic	65.0%
	GGGTCTACCAGTGGCAGCGA+CGG	-	chr7.4:85942074-85942093	MS.gene91652:CDS	65.0%
	TAGGTCGTGGTGGTTCCGCG+GGG	-	chr7.4:85942031-85942050	MS.gene91652:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	85941938	85942350	85941938	ID=MS.gene91652
chr7.4	mRNA	85941938	85942350	85941938	ID=MS.gene91652.t1;Parent=MS.gene91652
chr7.4	exon	85942338	85942350	85942338	ID=MS.gene91652.t1.exon1;Parent=MS.gene91652.t1
chr7.4	CDS	85942338	85942350	85942338	ID=cds.MS.gene91652.t1;Parent=MS.gene91652.t1
chr7.4	exon	85941938	85942254	85941938	ID=MS.gene91652.t1.exon2;Parent=MS.gene91652.t1
chr7.4	CDS	85941938	85942254	85941938	ID=cds.MS.gene91652.t1;Parent=MS.gene91652.t1

Gene Sequence

>MS.gene91652

ATGTCGAAGAGAGGTATGTACAGTGATTGTGAATCCTTTCTCAGATTCAATTGTATTGTTCTCTTTTTCTGATATGTATCCCTATTTTCTGATTAGGTCGTGGTGGTTCCGCGGGGAACAAGTTCCGTATGTCACTGGGTCTACCAGTGGCAGCGACGGTGAACTGTGCTGATAACACCGGAGCAAAGAATCTGTACATCATCTCCGTGAAGGGAATCAAGGGTAGGTTGAATCGTCTTCCTTCAGCTTGTGCTGGTGACGTGGTGATGGCTACTGTGAAGAAGGGAAAGCCTGATCTCAGGAAGAATGTTTTGCCAGCTGTCATCGTTCGTCAGCATAAGCCATGGCGCCGAAAGGACGGTGTCTTCATGTACTTTGAAGGTAACAAACGCTGTTTTGATTCATGTTTTTGA

Protein sequence

>MS.gene91652.t1

MSKRGRGGSAGNKFRMSLGLPVAATVNCADNTGAKNLYIISVKGIKGRLNRLPSACAGDVVMATVKKGKPDLRKNVLPAVIVRQHKPWRRKDGVFMYFEGNKRCFDSCF