Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96508.t1 | PNX95515.1 | 87.1 | 85 | 8 | 1 | 1 | 85 | 108 | 189 | 9.90E-30 | 139 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96508.t1 | Q9SJF1 | 60.5 | 81 | 30 | 1 | 1 | 79 | 217 | 297 | 2.2e-17 | 89.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96508.t1 | A0A2K3P2G1 | 87.1 | 85 | 8 | 1 | 1 | 85 | 50 | 131 | 7.1e-30 | 139.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049283 | MS.gene96508 | 0.83012 | 3.51E-55 | -1.69E-46 |
| MS.gene049419 | MS.gene96508 | -0.826539 | 2.57E-54 | -1.69E-46 |
| MS.gene050105 | MS.gene96508 | 0.864292 | 1.35E-64 | -1.69E-46 |
| MS.gene050312 | MS.gene96508 | 0.818587 | 1.81E-52 | -1.69E-46 |
| MS.gene050508 | MS.gene96508 | 0.829997 | 3.76E-55 | -1.69E-46 |
| MS.gene050571 | MS.gene96508 | 0.802541 | 5.34E-49 | -1.69E-46 |
| MS.gene050605 | MS.gene96508 | 0.808805 | 2.59E-50 | -1.69E-46 |
| MS.gene051064 | MS.gene96508 | 0.803571 | 3.27E-49 | -1.69E-46 |
| MS.gene051122 | MS.gene96508 | 0.806714 | 7.19E-50 | -1.69E-46 |
| MS.gene051368 | MS.gene96508 | 0.835384 | 1.73E-56 | -1.69E-46 |
| MS.gene051597 | MS.gene96508 | 0.821824 | 3.29E-53 | -1.69E-46 |
| MS.gene052333 | MS.gene96508 | 0.80935 | 1.98E-50 | -1.69E-46 |
| MS.gene052586 | MS.gene96508 | 0.82823 | 1.01E-54 | -1.69E-46 |
| MS.gene052954 | MS.gene96508 | 0.84136 | 4.98E-58 | -1.69E-46 |
| MS.gene053080 | MS.gene96508 | 0.810678 | 1.02E-50 | -1.69E-46 |
| MS.gene05308 | MS.gene96508 | 0.834393 | 3.08E-56 | -1.69E-46 |
| MS.gene053963 | MS.gene96508 | 0.829576 | 4.77E-55 | -1.69E-46 |
| MS.gene054328 | MS.gene96508 | 0.812882 | 3.39E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene96508 | 0.832654 | 8.35E-56 | -1.69E-46 |
| MS.gene055701 | MS.gene96508 | 0.825419 | 4.74E-54 | -1.69E-46 |
| MS.gene056045 | MS.gene96508 | 0.828456 | 8.90E-55 | -1.69E-46 |
| MS.gene056403 | MS.gene96508 | 0.809539 | 1.80E-50 | -1.69E-46 |
| MS.gene05659 | MS.gene96508 | 0.805628 | 1.22E-49 | -1.69E-46 |
| MS.gene056605 | MS.gene96508 | 0.816799 | 4.59E-52 | -1.69E-46 |
| MS.gene056973 | MS.gene96508 | 0.809997 | 1.43E-50 | -1.69E-46 |
| MS.gene057071 | MS.gene96508 | 0.805536 | 1.27E-49 | -1.69E-46 |
| MS.gene057208 | MS.gene96508 | 0.80802 | 3.80E-50 | -1.69E-46 |
| MS.gene058126 | MS.gene96508 | 0.803034 | 4.23E-49 | -1.69E-46 |
| MS.gene058223 | MS.gene96508 | -0.844291 | 8.28E-59 | -1.69E-46 |
| MS.gene058548 | MS.gene96508 | 0.806012 | 1.01E-49 | -1.69E-46 |
| MS.gene059935 | MS.gene96508 | 0.800386 | 1.48E-48 | -1.69E-46 |
| MS.gene060879 | MS.gene96508 | 0.818422 | 1.98E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 21 sgRNAs with CRISPR-Local
Find 22 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCATATATCTTCGTGTC+TGG | 0.316983 | 4.1:+47349008 | None:intergenic |
| AACCCAGACACGAAGATATA+TGG | 0.324106 | 4.1:-47349011 | MS.gene96508:CDS |
| TAAAGCTGCTACCGCAGTTT+TGG | 0.336125 | 4.1:-47349084 | MS.gene96508:CDS |
| ATGGTATTCTCTTTATCTTC+TGG | 0.358836 | 4.1:-47349112 | MS.gene96508:CDS |
| GTAGATATGAGAAGAAGTTC+AGG | 0.418084 | 4.1:-47348942 | MS.gene96508:CDS |
| CTATCGCTTCTGCCTCTGAT+TGG | 0.419536 | 4.1:+47348964 | None:intergenic |
| CAGACGACAATGCTTCTGTT+AGG | 0.424539 | 4.1:-47348905 | None:intergenic |
| TTCCATATATCTTCGTGTCT+GGG | 0.450384 | 4.1:+47349009 | None:intergenic |
| TTCTGCCTCTGATTGGAGGC+GGG | 0.477218 | 4.1:+47348971 | None:intergenic |
| CTTCTGCCTCTGATTGGAGG+CGG | 0.485826 | 4.1:+47348970 | None:intergenic |
| ATTGTCGTCTGAGGATTTAG+AGG | 0.499374 | 4.1:+47348917 | None:intergenic |
| TCGCTTCTGCCTCTGATTGG+AGG | 0.511592 | 4.1:+47348967 | None:intergenic |
| TGTTCCCCTGCAGAGAGAAG+TGG | 0.531572 | 4.1:-47349154 | None:intergenic |
| GCAGAATACTTCCGTGCTCA+TGG | 0.555358 | 4.1:-47349131 | MS.gene96508:CDS |
| TTTATCACAGGCTGATAACA+TGG | 0.568230 | 4.1:-47349045 | MS.gene96508:CDS |
| GTTCCCCTGCAGAGAGAAGT+GGG | 0.583589 | 4.1:-47349153 | None:intergenic |
| CTTAGCCCGCCTCCAATCAG+AGG | 0.603367 | 4.1:-47348976 | MS.gene96508:CDS |
| AACAGAAGCATTGTCGTCTG+AGG | 0.611947 | 4.1:+47348908 | None:intergenic |
| TAAAGAGAATACCATGAGCA+CGG | 0.615254 | 4.1:+47349120 | None:intergenic |
| CAGACACGAAGATATATGGA+AGG | 0.653054 | 4.1:-47349007 | MS.gene96508:CDS |
| AGACACGAAGATATATGGAA+GGG | 0.689139 | 4.1:-47349006 | MS.gene96508:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGGTATTCTCTTTATCTTC+TGG | - | chr4.1:47348935-47348954 | MS.gene96508:CDS | 30.0% | |
| AGACACGAAGATATATGGAA+GGG | - | chr4.1:47349041-47349060 | MS.gene96508:CDS | 35.0% | |
| GTAGATATGAGAAGAAGTTC+AGG | - | chr4.1:47349105-47349124 | MS.gene96508:CDS | 35.0% | |
| TAAAGAGAATACCATGAGCA+CGG | + | chr4.1:47348930-47348949 | None:intergenic | 35.0% | |
| TTCCATATATCTTCGTGTCT+GGG | + | chr4.1:47349041-47349060 | None:intergenic | 35.0% | |
| TTTATCACAGGCTGATAACA+TGG | - | chr4.1:47349002-47349021 | MS.gene96508:CDS | 35.0% | |
| AACCCAGACACGAAGATATA+TGG | - | chr4.1:47349036-47349055 | MS.gene96508:CDS | 40.0% | |
| ATTGTCGTCTGAGGATTTAG+AGG | + | chr4.1:47349133-47349152 | None:intergenic | 40.0% | |
| CAAAAAGCTCGGTTTATCAC+AGG | - | chr4.1:47348990-47349009 | MS.gene96508:CDS | 40.0% | |
| CAGACACGAAGATATATGGA+AGG | - | chr4.1:47349040-47349059 | MS.gene96508:CDS | 40.0% | |
| CTTCCATATATCTTCGTGTC+TGG | + | chr4.1:47349042-47349061 | None:intergenic | 40.0% | |
| ! | CTTTTTGCCTTCCAAAACTG+CGG | + | chr4.1:47348977-47348996 | None:intergenic | 40.0% |
| !!! | GTTTTGGAAGGCAAAAAGCT+CGG | - | chr4.1:47348979-47348998 | MS.gene96508:CDS | 40.0% |
| ! | TAAAGCTGCTACCGCAGTTT+TGG | - | chr4.1:47348963-47348982 | MS.gene96508:CDS | 45.0% |
| !! | AACAGAAGCATTGTCGTCTG+AGG | + | chr4.1:47349142-47349161 | None:intergenic | 45.0% |
| CTATCGCTTCTGCCTCTGAT+TGG | + | chr4.1:47349086-47349105 | None:intergenic | 50.0% | |
| GCAGAATACTTCCGTGCTCA+TGG | - | chr4.1:47348916-47348935 | MS.gene96508:CDS | 50.0% | |
| CTTCTGCCTCTGATTGGAGG+CGG | + | chr4.1:47349080-47349099 | None:intergenic | 55.0% | |
| TCGCTTCTGCCTCTGATTGG+AGG | + | chr4.1:47349083-47349102 | None:intergenic | 55.0% | |
| TTCTGCCTCTGATTGGAGGC+GGG | + | chr4.1:47349079-47349098 | None:intergenic | 55.0% | |
| !!! | GCTGCTACCGCAGTTTTGGA+AGG | - | chr4.1:47348967-47348986 | MS.gene96508:CDS | 55.0% |
| CTTAGCCCGCCTCCAATCAG+AGG | - | chr4.1:47349071-47349090 | MS.gene96508:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 47348906 | 47349163 | 47348906 | ID=MS.gene96508 |
| chr4.1 | mRNA | 47348906 | 47349163 | 47348906 | ID=MS.gene96508.t1;Parent=MS.gene96508 |
| chr4.1 | exon | 47348906 | 47349163 | 47348906 | ID=MS.gene96508.t1.exon1;Parent=MS.gene96508.t1 |
| chr4.1 | CDS | 47348906 | 47349163 | 47348906 | ID=cds.MS.gene96508.t1;Parent=MS.gene96508.t1 |
| Gene Sequence |
| Protein sequence |
