AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97237


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97237.t1 MTR_1g041155 92.500 240 13 3 1 237 120 357 1.68e-156 438
MS.gene97237.t1 MTR_1g076500 63.333 240 78 5 1 230 142 381 7.26e-97 288
MS.gene97237.t1 MTR_7g095960 48.954 239 110 6 2 237 127 356 1.19e-70 220
MS.gene97237.t1 MTR_1g070970 50.211 237 91 6 2 235 136 348 5.91e-67 211
MS.gene97237.t1 MTR_8g093440 40.845 142 77 1 34 168 20 161 3.52e-38 132
MS.gene97237.t1 MTR_8g093440 40.845 142 77 1 34 168 82 223 1.05e-37 133
MS.gene97237.t1 MTR_7g083080 47.656 128 61 2 33 154 129 256 9.39e-37 132
MS.gene97237.t1 MTR_4g132963 44.915 118 65 0 36 153 117 234 1.75e-36 130
MS.gene97237.t1 MTR_4g132940 44.915 118 65 0 36 153 117 234 1.75e-36 130
MS.gene97237.t1 MTR_4g132970 39.850 133 80 0 36 168 138 270 5.25e-36 129
MS.gene97237.t1 MTR_2g025520 43.478 138 74 1 42 179 142 275 8.36e-35 126
MS.gene97237.t1 MTR_2g013780 40.625 128 70 2 34 155 143 270 9.88e-34 124
MS.gene97237.t1 MTR_4g078310 39.683 126 67 2 34 151 119 243 1.23e-31 117
MS.gene97237.t1 MTR_4g088770 37.795 127 73 2 34 154 149 275 2.58e-31 117
MS.gene97237.t1 MTR_4g088760 37.795 127 73 2 34 154 202 328 7.34e-31 117
MS.gene97237.t1 MTR_2g013880 29.560 159 106 2 7 159 116 274 6.11e-28 108
MS.gene97237.t1 MTR_7g053310 35.714 140 78 3 22 155 114 247 4.56e-27 105
MS.gene97237.t1 MTR_8g012240 33.766 154 89 3 27 173 130 277 1.91e-26 104
MS.gene97237.t1 MTR_5g008720 34.677 124 75 1 34 151 97 220 1.58e-24 98.2
MS.gene97237.t1 MTR_1g103610 71.795 39 11 0 136 174 1 39 8.61e-14 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97237.t1 AT3G22970 51.200 250 94 5 1 237 25 259 4.85e-76 231
MS.gene97237.t1 AT4G14620 53.782 238 96 4 2 235 112 339 3.25e-75 231
MS.gene97237.t1 AT3G22970 51.406 249 93 5 2 237 137 370 2.61e-74 230
MS.gene97237.t1 AT2G38820 48.691 191 87 3 1 187 126 309 6.52e-62 196
MS.gene97237.t1 AT3G54550 45.860 157 77 3 1 153 115 267 6.44e-47 157
MS.gene97237.t1 AT3G07350 37.748 151 82 2 27 171 134 278 7.97e-35 126
MS.gene97237.t1 AT2G39650 43.902 123 63 1 38 154 52 174 1.21e-33 121
MS.gene97237.t1 AT2G39650 43.902 123 63 1 38 154 114 236 4.07e-33 121
MS.gene97237.t1 AT3G25240 40.523 153 82 3 11 154 90 242 2.83e-32 119
MS.gene97237.t1 AT4G32480 39.516 124 69 2 38 155 120 243 7.45e-29 110
MS.gene97237.t1 AT2G20670 39.474 114 63 1 46 153 130 243 1.51e-27 107
MS.gene97237.t1 AT1G62420 38.261 115 68 1 42 153 116 230 1.72e-27 106
MS.gene97237.t1 AT1G77145 35.000 140 73 2 31 152 72 211 1.75e-26 103
MS.gene97237.t1 AT1G12030 38.938 113 68 1 42 153 118 230 2.27e-26 103
MS.gene97237.t1 AT1G77160 34.247 146 78 2 31 158 22 167 3.31e-26 101
MS.gene97237.t1 AT1G77160 34.247 146 78 2 31 158 77 222 6.13e-26 102
MS.gene97237.t1 AT1G77145 39.583 96 48 1 67 152 59 154 6.34e-21 87.4
MS.gene97237.t1 AT2G38820 48.438 64 33 0 2 65 127 190 3.03e-13 67.0

Find 52 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCATTTGCAGATCAAAA+TGG 0.248714 1.3:-23972063 MS.gene97237:CDS
GCCGCATCGGAGACTTGCTT+TGG 0.348894 1.3:+23971651 None:intergenic
AAGAAAAGAAGATTTGATTA+AGG 0.362890 1.3:-23972122 MS.gene97237:CDS
TCGGAGGTGGATAGTGATTC+TGG 0.369464 1.3:-23971609 MS.gene97237:CDS
GAGTCCAGCGTGGCAGTTAC+CGG 0.378357 1.3:-23971508 MS.gene97237:CDS
GCTGAAAAGCTTCAATCTCT+TGG 0.409442 1.3:-23972094 MS.gene97237:CDS
ATTCCTCTTTCACCACTCTT+CGG 0.422133 1.3:+23971477 None:intergenic
TGCTTTGGCCTAGCTGCCGC+CGG 0.433998 1.3:+23971666 None:intergenic
AGTCTCCGATGCGGCGGCGG+TGG 0.440423 1.3:-23971643 MS.gene97237:CDS
ATCCATTTGCAGATCAAAAT+GGG 0.441823 1.3:-23972062 MS.gene97237:CDS
GGATAGTGATTCTGGTGAAT+TGG 0.451879 1.3:-23971601 MS.gene97237:CDS
AAAACAAATTCTTGTCCTGC+TGG 0.468712 1.3:-23972037 MS.gene97237:intron
CCACTCTTCGGTTTCACCGC+CGG 0.488504 1.3:+23971489 None:intergenic
GCAAGTCTCCGATGCGGCGG+CGG 0.496129 1.3:-23971646 MS.gene97237:CDS
GAGTGGTGAAAGAGGAATGA+AGG 0.505285 1.3:-23971472 MS.gene97237:CDS
TGGATTAGCTTCTTTGCTGA+AGG 0.511501 1.3:-23971445 MS.gene97237:CDS
GGAGAAAGCTTCGCCGGAGG+AGG 0.512134 1.3:-23971562 MS.gene97237:CDS
TTCGCCGGAGGAGGTAGTTA+CGG 0.512281 1.3:-23971553 MS.gene97237:CDS
AAGCAGAGTTTGAAGAAGAA+AGG 0.517627 1.3:-23971744 MS.gene97237:CDS
ACCGCCGGTAACTGCCACGC+TGG 0.521213 1.3:+23971504 None:intergenic
GGATCTCTCCGGCGGCAGCT+AGG 0.528128 1.3:-23971674 MS.gene97237:CDS
TGTTTCCACCGCCGCCGCAT+CGG 0.530760 1.3:+23971638 None:intergenic
GGAAACAGTTTCATCGGAGG+TGG 0.532775 1.3:-23971622 MS.gene97237:CDS
CGTCACCGTCATGTCGGAGG+CGG 0.549114 1.3:-23971769 MS.gene97237:CDS
TCAATTCATTGATGCGATTG+TGG 0.553988 1.3:-23971925 MS.gene97237:CDS
TGGTGAGGAGAAAGCTTCGC+CGG 0.554242 1.3:-23971568 MS.gene97237:CDS
AAACCGAAGAGTGGTGAAAG+AGG 0.554832 1.3:-23971480 MS.gene97237:CDS
GAAAGAGGAATGAAGGTGAC+TGG 0.556629 1.3:-23971465 MS.gene97237:CDS
GGCGGTGGAAACAGTTTCAT+CGG 0.562645 1.3:-23971628 MS.gene97237:CDS
CGTTCTTCAACACTTACACA+AGG 0.565445 1.3:+23972199 None:intergenic
CTTGTGTAAGTGTTGAAGAA+CGG 0.570166 1.3:-23972198 MS.gene97237:CDS
ATGTAATCGCGCTTCCTCCA+CGG 0.582747 1.3:+23971708 None:intergenic
CTTCGCCGCCTCCGACATGA+CGG 0.584516 1.3:+23971764 None:intergenic
TTCATTGATGCGATTGTGGA+AGG 0.586622 1.3:-23971921 MS.gene97237:CDS
GCCGCCGTAACTACCTCCTC+CGG 0.598847 1.3:+23971549 None:intergenic
GTTAGCGAAGTGGATCTCTC+CGG 0.616423 1.3:-23971685 MS.gene97237:CDS
AAAGCAAGTCTCCGATGCGG+CGG 0.623703 1.3:-23971649 MS.gene97237:CDS
GAATGCATGTTCCGCCGTGG+AGG 0.625206 1.3:-23971722 MS.gene97237:CDS
GCAGATCGTCACCGTCATGT+CGG 0.626575 1.3:-23971775 MS.gene97237:CDS
GCGATTACATGTTAGCGAAG+TGG 0.650775 1.3:-23971695 MS.gene97237:CDS
GATCGTCACCGTCATGTCGG+AGG 0.651203 1.3:-23971772 MS.gene97237:CDS
TCCAGCGTGGCAGTTACCGG+CGG 0.657779 1.3:-23971505 MS.gene97237:CDS
AGCGAAGTGGATCTCTCCGG+CGG 0.660131 1.3:-23971682 MS.gene97237:CDS
CCGGCGGTGAAACCGAAGAG+TGG 0.661349 1.3:-23971489 MS.gene97237:CDS
AAGGAATGCATGTTCCGCCG+TGG 0.669326 1.3:-23971725 MS.gene97237:CDS
GGCGGCGACAGCGACAGCGA+CGG 0.672784 1.3:-23971532 MS.gene97237:CDS
GCCGGAGGAGGTAGTTACGG+CGG 0.688861 1.3:-23971550 MS.gene97237:CDS
GGTGGAAACAGTTTCATCGG+AGG 0.697111 1.3:-23971625 MS.gene97237:CDS
TGAGGAGAAAGCTTCGCCGG+AGG 0.709522 1.3:-23971565 MS.gene97237:CDS
GCCAAAGCAAGTCTCCGATG+CGG 0.718766 1.3:-23971652 MS.gene97237:CDS
CAGCGACGGTGAGTCCAGCG+TGG 0.734694 1.3:-23971518 MS.gene97237:CDS
TAATCGCGCTTCCTCCACGG+CGG 0.770748 1.3:+23971711 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGAAAAGAAGATTTGATTA+AGG - chr1.3:23971520-23971539 MS.gene97237:CDS 20.0%
!!! ATCGTTTTTTGTTTTGTTTT+AGG - chr1.3:23971688-23971707 MS.gene97237:CDS 20.0%
!!! TATTGATATCGATTTTCGAT+CGG - chr1.3:23971756-23971775 MS.gene97237:CDS 25.0%
ATCCATTTGCAGATCAAAAT+GGG - chr1.3:23971580-23971599 MS.gene97237:CDS 30.0%
TTAGAGAATTAATTACCAGC+AGG + chr1.3:23971623-23971642 None:intergenic 30.0%
! TTTTTCCCGACGAAAATATA+CGG + chr1.3:23971835-23971854 None:intergenic 30.0%
AAAACAAATTCTTGTCCTGC+TGG - chr1.3:23971605-23971624 MS.gene97237:CDS 35.0%
AAGCAGAGTTTGAAGAAGAA+AGG - chr1.3:23971898-23971917 MS.gene97237:CDS 35.0%
CATCCATTTGCAGATCAAAA+TGG - chr1.3:23971579-23971598 MS.gene97237:CDS 35.0%
TCAATTCATTGATGCGATTG+TGG - chr1.3:23971717-23971736 MS.gene97237:CDS 35.0%
! GTATATTTTCGTCGGGAAAA+AGG - chr1.3:23971834-23971853 MS.gene97237:CDS 35.0%
!! CTTGTGTAAGTGTTGAAGAA+CGG - chr1.3:23971444-23971463 MS.gene97237:CDS 35.0%
ATTCCTCTTTCACCACTCTT+CGG + chr1.3:23972168-23972187 None:intergenic 40.0%
CGTTCTTCAACACTTACACA+AGG + chr1.3:23971446-23971465 None:intergenic 40.0%
GCTGAAAAGCTTCAATCTCT+TGG - chr1.3:23971548-23971567 MS.gene97237:CDS 40.0%
GGATAGTGATTCTGGTGAAT+TGG - chr1.3:23972041-23972060 MS.gene97237:CDS 40.0%
TTCATTGATGCGATTGTGGA+AGG - chr1.3:23971721-23971740 MS.gene97237:CDS 40.0%
! TCGCTTCCGTATATTTTCGT+CGG - chr1.3:23971826-23971845 MS.gene97237:CDS 40.0%
!! CTCCCATTTTGATCTGCAAA+TGG + chr1.3:23971585-23971604 None:intergenic 40.0%
!! GGTGAATTGGAGCTGATTTT+TGG - chr1.3:23972054-23972073 MS.gene97237:CDS 40.0%
!! TGGATTAGCTTCTTTGCTGA+AGG - chr1.3:23972197-23972216 MS.gene97237:CDS 40.0%
!!! ATTGGAGCTGATTTTTGGTG+AGG - chr1.3:23972059-23972078 MS.gene97237:CDS 40.0%
AAACCGAAGAGTGGTGAAAG+AGG - chr1.3:23972162-23972181 MS.gene97237:CDS 45.0%
GAAAGAGGAATGAAGGTGAC+TGG - chr1.3:23972177-23972196 MS.gene97237:CDS 45.0%
GAGTGGTGAAAGAGGAATGA+AGG - chr1.3:23972170-23972189 MS.gene97237:CDS 45.0%
GCGATTACATGTTAGCGAAG+TGG - chr1.3:23971947-23971966 MS.gene97237:intron 45.0%
! CGCTTCCGTATATTTTCGTC+GGG - chr1.3:23971827-23971846 MS.gene97237:CDS 45.0%
! TTTTCGTCGGGAAAAAGGAG+CGG - chr1.3:23971839-23971858 MS.gene97237:CDS 45.0%
ATGTAATCGCGCTTCCTCCA+CGG + chr1.3:23971937-23971956 None:intergenic 50.0%
GGAAACAGTTTCATCGGAGG+TGG - chr1.3:23972020-23972039 MS.gene97237:intron 50.0%
GGCGGTGGAAACAGTTTCAT+CGG - chr1.3:23972014-23972033 MS.gene97237:intron 50.0%
GGTGGAAACAGTTTCATCGG+AGG - chr1.3:23972017-23972036 MS.gene97237:intron 50.0%
GTTAGCGAAGTGGATCTCTC+CGG - chr1.3:23971957-23971976 MS.gene97237:intron 50.0%
TCGGAGGTGGATAGTGATTC+TGG - chr1.3:23972033-23972052 MS.gene97237:intron 50.0%
AAAGCAAGTCTCCGATGCGG+CGG - chr1.3:23971993-23972012 MS.gene97237:intron 55.0%
AAGGAATGCATGTTCCGCCG+TGG - chr1.3:23971917-23971936 MS.gene97237:CDS 55.0%
GCAGATCGTCACCGTCATGT+CGG - chr1.3:23971867-23971886 MS.gene97237:CDS 55.0%
GCCAAAGCAAGTCTCCGATG+CGG - chr1.3:23971990-23972009 MS.gene97237:intron 55.0%
TGGTGAGGAGAAAGCTTCGC+CGG - chr1.3:23972074-23972093 MS.gene97237:CDS 55.0%
TTCGCCGGAGGAGGTAGTTA+CGG - chr1.3:23972089-23972108 MS.gene97237:CDS 55.0%
AGCGAAGTGGATCTCTCCGG+CGG - chr1.3:23971960-23971979 MS.gene97237:intron 60.0%
CCACTCTTCGGTTTCACCGC+CGG + chr1.3:23972156-23972175 None:intergenic 60.0%
GAATGCATGTTCCGCCGTGG+AGG - chr1.3:23971920-23971939 MS.gene97237:CDS 60.0%
GAGTCCAGCGTGGCAGTTAC+CGG - chr1.3:23972134-23972153 MS.gene97237:CDS 60.0%
GATCGTCACCGTCATGTCGG+AGG - chr1.3:23971870-23971889 MS.gene97237:CDS 60.0%
TAATCGCGCTTCCTCCACGG+CGG + chr1.3:23971934-23971953 None:intergenic 60.0%
TGAGGAGAAAGCTTCGCCGG+AGG - chr1.3:23972077-23972096 MS.gene97237:CDS 60.0%
! GCCGCATCGGAGACTTGCTT+TGG + chr1.3:23971994-23972013 None:intergenic 60.0%
CCGGCGGTGAAACCGAAGAG+TGG - chr1.3:23972153-23972172 MS.gene97237:CDS 65.0%
CGTCACCGTCATGTCGGAGG+CGG - chr1.3:23971873-23971892 MS.gene97237:CDS 65.0%
CTTCGCCGCCTCCGACATGA+CGG + chr1.3:23971881-23971900 None:intergenic 65.0%
GCCGCCGTAACTACCTCCTC+CGG + chr1.3:23972096-23972115 None:intergenic 65.0%
GCCGGAGGAGGTAGTTACGG+CGG - chr1.3:23972092-23972111 MS.gene97237:CDS 65.0%
GGAGAAAGCTTCGCCGGAGG+AGG - chr1.3:23972080-23972099 MS.gene97237:CDS 65.0%
TCCAGCGTGGCAGTTACCGG+CGG - chr1.3:23972137-23972156 MS.gene97237:CDS 65.0%
TGCTTTGGCCTAGCTGCCGC+CGG + chr1.3:23971979-23971998 None:intergenic 65.0%
TGTTTCCACCGCCGCCGCAT+CGG + chr1.3:23972007-23972026 None:intergenic 65.0%
ACCGCCGGTAACTGCCACGC+TGG + chr1.3:23972141-23972160 None:intergenic 70.0%
CAGCGACGGTGAGTCCAGCG+TGG - chr1.3:23972124-23972143 MS.gene97237:CDS 70.0%
GCAAGTCTCCGATGCGGCGG+CGG - chr1.3:23971996-23972015 MS.gene97237:intron 70.0%
GGATCTCTCCGGCGGCAGCT+AGG - chr1.3:23971968-23971987 MS.gene97237:intron 70.0%
AGTCTCCGATGCGGCGGCGG+TGG - chr1.3:23971999-23972018 MS.gene97237:intron 75.0%
GGCGGCGACAGCGACAGCGA+CGG - chr1.3:23972110-23972129 MS.gene97237:CDS 75.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 23971434 23972230 23971434 ID=MS.gene97237
chr1.3 mRNA 23971434 23972230 23971434 ID=MS.gene97237.t1;Parent=MS.gene97237
chr1.3 exon 23972038 23972230 23972038 ID=MS.gene97237.t1.exon1;Parent=MS.gene97237.t1
chr1.3 CDS 23972038 23972230 23972038 ID=cds.MS.gene97237.t1;Parent=MS.gene97237.t1
chr1.3 exon 23971434 23971954 23971434 ID=MS.gene97237.t1.exon2;Parent=MS.gene97237.t1
chr1.3 CDS 23971434 23971954 23971434 ID=cds.MS.gene97237.t1;Parent=MS.gene97237.t1
Gene Sequence

>MS.gene97237

AATTTGATTCCTTGTGTAAGTGTTGAAGAACGGAATTTACTAGCAGATGCAACGAAAATTGTTGAGAAGAATAGCAAAATTTACAAAAGAAAAGAAGATTTGATTAAGGTCGTTGCTGAAAAGCTTCAATCTCTTGGTTACGATTCATCCATTTGCAGATCAAAATGGGAGAAAACAAATTCTTGTCCTGCTGGTAATTAATTCTCTAATCAGATTCAAAATTCAAAATTGAGTTAACTCAACTCGTTTTCTGAATCGTTTTTTGTTTTGTTTTAGGTGATTATCAATTCATTGATGCGATTGTGGAAGGTGAAACGTTGATTATTGATATCGATTTTCGATCGGAGTTTGAAATCGCGAGATCTACAAGTCAGTATAAGACGATTCTTCAATCGCTTCCGTATATTTTCGTCGGGAAAAAGGAGCGGTTGAAGCAGATCGTCACCGTCATGTCGGAGGCGGCGAAGCAGAGTTTGAAGAAGAAAGGAATGCATGTTCCGCCGTGGAGGAAGCGCGATTACATGTTAGCGAAGTGGATCTCTCCGGCGGCAGCTAGGCCAAAGCAAGTCTCCGATGCGGCGGCGGTGGAAACAGTTTCATCGGAGGTGGATAGTGATTCTGGTGAATTGGAGCTGATTTTTGGTGAGGAGAAAGCTTCGCCGGAGGAGGTAGTTACGGCGGCGACAGCGACAGCGACGGTGAGTCCAGCGTGGCAGTTACCGGCGGTGAAACCGAAGAGTGGTGAAAGAGGAATGAAGGTGACTGGATTAGCTTCTTTGCTGAAGGAGAAACCATAG

Protein sequence

>MS.gene97237.t1

NLIPCVSVEERNLLADATKIVEKNSKIYKRKEDLIKVVAEKLQSLGYDSSICRSKWEKTNSCPAGDYQFIDAIVEGETLIIDIDFRSEFEIARSTSQYKTILQSLPYIFVGKKERLKQIVTVMSEAAKQSLKKKGMHVPPWRKRDYMLAKWISPAAARPKQVSDAAAVETVSSEVDSDSGELELIFGEEKASPEEVVTAATATATVSPAWQLPAVKPKSGERGMKVTGLASLLKEKP