AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059870


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059870.t1 MTR_2g080040 91.981 212 11 3 1 209 1 209 6.71e-126 360
MS.gene059870.t1 MTR_2g090155 72.152 79 22 0 131 209 77 155 2.35e-36 130
MS.gene059870.t1 MTR_2g090155 71.605 81 23 0 131 211 77 157 2.61e-36 130
MS.gene059870.t1 MTR_2g090155 72.152 79 22 0 131 209 77 155 3.04e-36 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059870.t1 AT1G52870 52.113 213 83 6 2 209 4 202 2.59e-62 196
MS.gene059870.t1 AT1G52870 52.113 213 83 6 2 209 4 202 1.38e-61 196
MS.gene059870.t1 AT4G03410 69.663 89 27 0 121 209 72 160 9.01e-41 141
MS.gene059870.t1 AT4G03410 69.663 89 27 0 121 209 72 160 1.12e-40 142

Find 39 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTACTATTGATAGTGATTAT+TGG 0.193346 2.2:+15326904 MS.gene059870:CDS
GCTAGTATTGTTCTTGTTGC+TGG 0.288636 2.2:+15326858 MS.gene059870:CDS
ACGACGCAAGTGAATGGATT+TGG 0.380452 2.2:+15326720 MS.gene059870:CDS
ACACAACTGGTTAGCTTATG+AGG 0.392465 2.2:+15327797 MS.gene059870:CDS
TTGTTTATTCTGTAGGAGAC+TGG 0.418497 2.2:+15327868 MS.gene059870:CDS
TCACTATCAAACCTTCTTGC+TGG 0.438486 2.2:-15326653 None:intergenic
TTGCCAAGATGGTGATTAGC+GGG 0.450889 2.2:+15327844 MS.gene059870:CDS
TTAGTGGAGAATCTTGATAC+TGG 0.460029 2.2:-15326540 None:intergenic
TGAAGAGAATGTGGAGAGTG+TGG 0.488302 2.2:+15326809 MS.gene059870:CDS
GATATGCACCTCTACACAAC+TGG 0.490397 2.2:+15327784 MS.gene059870:CDS
AATGGATTTGGAGCGAATGA+AGG 0.492577 2.2:+15326732 MS.gene059870:CDS
TCGAGTGCTTCGGGTTTGGT+TGG 0.494003 2.2:+15326777 MS.gene059870:CDS
GTGACGACGACGCAAGTGAA+TGG 0.496360 2.2:+15326714 MS.gene059870:CDS
AACCCCGCTAATCACCATCT+TGG 0.520776 2.2:-15327847 None:intergenic
GAAGGTTTGTTATCGTTGGA+AGG 0.524445 2.2:+15326750 MS.gene059870:CDS
AGCGGGGTTGTTTATTCTGT+AGG 0.524576 2.2:+15327861 MS.gene059870:CDS
GATAGTGAGCCGTGTGGAGA+TGG 0.547654 2.2:+15326668 MS.gene059870:CDS
GGAAGCTCTCAAGACAAATC+CGG 0.548802 2.2:+15327818 MS.gene059870:CDS
GAGGAAAGTGTGGTGAGGTA+CGG 0.563573 2.2:-15326466 None:intergenic
GAATGAAGGTTTGTTATCGT+TGG 0.563825 2.2:+15326746 MS.gene059870:CDS
GATATTACAGACCAGCAAGA+AGG 0.585948 2.2:+15326642 MS.gene059870:CDS
TTGGTGTGGAAATTGAAGGG+AGG 0.587765 2.2:-15326485 None:intergenic
TTTGCCAAGATGGTGATTAG+CGG 0.591010 2.2:+15327843 MS.gene059870:CDS
TAATGAGAATGAAGAGAATG+TGG 0.602012 2.2:+15326800 MS.gene059870:CDS
TGTAGGAGACTGGATTGCAC+AGG 0.610678 2.2:+15327878 MS.gene059870:CDS
AGGTGGAGATGGTGAATTAG+CGG 0.614566 2.2:+15326692 MS.gene059870:CDS
GTGGAGATGGAAGGTGGAGA+TGG 0.619496 2.2:+15326681 MS.gene059870:CDS
GTGAGGTACGGTGCTGTGAA+CGG 0.621994 2.2:-15326454 None:intergenic
TAAGCTAACCAGTTGTGTAG+AGG 0.623564 2.2:-15327792 None:intergenic
AGCCGTGTGGAGATGGAAGG+TGG 0.639866 2.2:+15326675 MS.gene059870:CDS
CTCCACCTTCCATCTCCACA+CGG 0.651428 2.2:-15326677 None:intergenic
AAATTGAAGGGAGGAAAGTG+TGG 0.659597 2.2:-15326476 None:intergenic
GAATTTCACAAAACGTGCAG+CGG 0.660249 2.2:+15326566 MS.gene059870:CDS
GTGAGCCGTGTGGAGATGGA+AGG 0.660357 2.2:+15326672 MS.gene059870:CDS
GAAGGGAGGAAAGTGTGGTG+AGG 0.665797 2.2:-15326471 None:intergenic
TGCCAAGATGGTGATTAGCG+GGG 0.667655 2.2:+15327845 MS.gene059870:CDS
GGTAGCGACGAAAGTATCAA+CGG 0.696411 2.2:+15326587 MS.gene059870:CDS
AGGTTTGATAGTGAGCCGTG+TGG 0.739464 2.2:+15326662 MS.gene059870:CDS
AGCGACGAAAGTATCAACGG+AGG 0.758121 2.2:+15326590 MS.gene059870:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAATAAAACATAACGTAA+AGG - chr2.2:15327126-15327145 None:intergenic 15.0%
!! CATATGGAAAAATATTTGTT+TGG + chr2.2:15327454-15327473 MS.gene059870:intron 20.0%
!! GATTTGGAAATGATTAAATT+TGG + chr2.2:15326993-15327012 MS.gene059870:intron 20.0%
!! TAAACTTCTCATTCAAAATT+AGG + chr2.2:15327365-15327384 MS.gene059870:intron 20.0%
!!! AAATATTTTTCCATATGCAT+TGG - chr2.2:15327451-15327470 None:intergenic 20.0%
!!! AATTTGCAATTTTATAGACT+AGG + chr2.2:15327041-15327060 MS.gene059870:intron 20.0%
!!! CAACTTTAAATTAGCTTATA+AGG - chr2.2:15327595-15327614 None:intergenic 20.0%
!!! CTTATAAGCTAATTTAAAGT+TGG + chr2.2:15327593-15327612 MS.gene059870:intron 20.0%
!!! TTACTATTGATAGTGATTAT+TGG + chr2.2:15326904-15326923 MS.gene059870:CDS 20.0%
! CAAGATAATCAAATTTGACT+GGG - chr2.2:15327693-15327712 None:intergenic 25.0%
! TATTATTCATAAGCTTGTCA+AGG - chr2.2:15327233-15327252 None:intergenic 25.0%
! TCAAGATAATCAAATTTGAC+TGG - chr2.2:15327694-15327713 None:intergenic 25.0%
!! CACGTTTAAATTAGCTTATT+TGG - chr2.2:15327638-15327657 None:intergenic 25.0%
!!! TTGGAAATGGATTTTGATTT+TGG - chr2.2:15326507-15326526 None:intergenic 25.0%
AAAATAAGTCAATCCAAACC+TGG - chr2.2:15327067-15327086 None:intergenic 30.0%
AATCACTATACTGTTTGGAT+TGG - chr2.2:15327308-15327327 None:intergenic 30.0%
TAATGAGAATGAAGAGAATG+TGG + chr2.2:15326800-15326819 MS.gene059870:CDS 30.0%
TCCAAACAGTATAGTGATTA+TGG + chr2.2:15327309-15327328 MS.gene059870:intron 30.0%
! TGAATTTTTCCTTATCTGCT+AGG + chr2.2:15327711-15327730 MS.gene059870:intron 30.0%
!! ATTTTGGTGTGGAAATTGAA+GGG - chr2.2:15326491-15326510 None:intergenic 30.0%
!! TTTTGTGAAATTCGGATTAG+TGG - chr2.2:15326559-15326578 None:intergenic 30.0%
!!! AATGGATTTTGATTTTGGTG+TGG - chr2.2:15326502-15326521 None:intergenic 30.0%
!!! CGAAGAACTATACTTTTTCT+TGG - chr2.2:15327557-15327576 None:intergenic 30.0%
!!! GTTTTTAGCATATGCAAAAC+TGG - chr2.2:15327281-15327300 None:intergenic 30.0%
AACACAAATCCTAGCAGATA+AGG - chr2.2:15327723-15327742 None:intergenic 35.0%
AGTCTATACATTTGCATGCA+GGG + chr2.2:15327522-15327541 MS.gene059870:intron 35.0%
CAAATTTGACTGGGTATACA+GGG - chr2.2:15327684-15327703 None:intergenic 35.0%
GCCATAATCACTATACTGTT+TGG - chr2.2:15327313-15327332 None:intergenic 35.0%
GGCTAATCTAAATTAGCAAC+TGG - chr2.2:15327663-15327682 None:intergenic 35.0%
TAGTCTATACATTTGCATGC+AGG + chr2.2:15327521-15327540 MS.gene059870:intron 35.0%
TCAAATTTGACTGGGTATAC+AGG - chr2.2:15327685-15327704 None:intergenic 35.0%
TGTGTGTGATTCTTTGGATT+TGG + chr2.2:15326977-15326996 MS.gene059870:intron 35.0%
TTAGGTCATAGTTACCAACT+TGG + chr2.2:15327383-15327402 MS.gene059870:intron 35.0%
TTAGTGGAGAATCTTGATAC+TGG - chr2.2:15326543-15326562 None:intergenic 35.0%
TTGTTTATTCTGTAGGAGAC+TGG + chr2.2:15327868-15327887 MS.gene059870:CDS 35.0%
! CTTGATACTGGAAAGTTTTG+TGG - chr2.2:15326531-15326550 None:intergenic 35.0%
!! AAAGTTTTGTGGCTTGGAAA+TGG - chr2.2:15326520-15326539 None:intergenic 35.0%
!! GAATGAAGGTTTGTTATCGT+TGG + chr2.2:15326746-15326765 MS.gene059870:CDS 35.0%
!! GATTTTGGTGTGGAAATTGA+AGG - chr2.2:15326492-15326511 None:intergenic 35.0%
AAATTGAAGGGAGGAAAGTG+TGG - chr2.2:15326479-15326498 None:intergenic 40.0%
ATCACCATCTTGGCAAAAAC+CGG - chr2.2:15327840-15327859 None:intergenic 40.0%
CTGCTAGGATTTGTGTTTCT+TGG + chr2.2:15327726-15327745 MS.gene059870:intron 40.0%
GAATTTCACAAAACGTGCAG+CGG + chr2.2:15326566-15326585 MS.gene059870:CDS 40.0%
GATATTACAGACCAGCAAGA+AGG + chr2.2:15326642-15326661 MS.gene059870:CDS 40.0%
GCTAGAAATCGACACAAACT+TGG - chr2.2:15327335-15327354 None:intergenic 40.0%
GCTAGTATTGTTCTTGTTGC+TGG + chr2.2:15326858-15326877 MS.gene059870:CDS 40.0%
TAAGCTAACCAGTTGTGTAG+AGG - chr2.2:15327795-15327814 None:intergenic 40.0%
TATACATTTGCATGCAGGGA+GGG + chr2.2:15327526-15327545 MS.gene059870:intron 40.0%
TCACTATCAAACCTTCTTGC+TGG - chr2.2:15326656-15326675 None:intergenic 40.0%
TGTGTGTGTGTGTGATTCTT+TGG + chr2.2:15326971-15326990 MS.gene059870:intron 40.0%
TTTATAGACTAGGCCAGGTT+TGG + chr2.2:15327051-15327070 MS.gene059870:intron 40.0%
TTTGCCAAGATGGTGATTAG+CGG + chr2.2:15327843-15327862 MS.gene059870:CDS 40.0%
! AAATCCGGTTTTTGCCAAGA+TGG + chr2.2:15327833-15327852 MS.gene059870:CDS 40.0%
! AATGGATTTGGAGCGAATGA+AGG + chr2.2:15326732-15326751 MS.gene059870:CDS 40.0%
! GCAATTTTATAGACTAGGCC+AGG + chr2.2:15327046-15327065 MS.gene059870:intron 40.0%
! GCTGCACGTTTTGTGAAATT+CGG - chr2.2:15326567-15326586 None:intergenic 40.0%
! TTTCCATATGCATTGGCCAA+GGG - chr2.2:15327444-15327463 None:intergenic 40.0%
!! ACACAACTGGTTAGCTTATG+AGG + chr2.2:15327797-15327816 MS.gene059870:CDS 40.0%
!! GAAGGTTTGTTATCGTTGGA+AGG + chr2.2:15326750-15326769 MS.gene059870:CDS 40.0%
!! GTGTTTCTTGGTGCTATGTA+GGG + chr2.2:15327738-15327757 MS.gene059870:intron 40.0%
!! GTTTGTGTCGATTTCTAGCT+TGG + chr2.2:15327336-15327355 MS.gene059870:intron 40.0%
!! TACTGGAAAGTTTTGTGGCT+TGG - chr2.2:15326526-15326545 None:intergenic 40.0%
!! TGTGTTTCTTGGTGCTATGT+AGG + chr2.2:15327737-15327756 MS.gene059870:intron 40.0%
!! TTTTCCATATGCATTGGCCA+AGG - chr2.2:15327445-15327464 None:intergenic 40.0%
AGCGGGGTTGTTTATTCTGT+AGG + chr2.2:15327861-15327880 MS.gene059870:CDS 45.0%
AGGTGGAGATGGTGAATTAG+CGG + chr2.2:15326692-15326711 MS.gene059870:CDS 45.0%
CTATACATTTGCATGCAGGG+AGG + chr2.2:15327525-15327544 MS.gene059870:intron 45.0%
GATATGCACCTCTACACAAC+TGG + chr2.2:15327784-15327803 MS.gene059870:CDS 45.0%
GGAAGCTCTCAAGACAAATC+CGG + chr2.2:15327818-15327837 MS.gene059870:CDS 45.0%
TGAAGAGAATGTGGAGAGTG+TGG + chr2.2:15326809-15326828 MS.gene059870:CDS 45.0%
TTGCCAAGATGGTGATTAGC+GGG + chr2.2:15327844-15327863 MS.gene059870:CDS 45.0%
TTGGTGTGGAAATTGAAGGG+AGG - chr2.2:15326488-15326507 None:intergenic 45.0%
! ACGACGCAAGTGAATGGATT+TGG + chr2.2:15326720-15326739 MS.gene059870:CDS 45.0%
!! ACTTGAGTTGATCGATCCCT+TGG + chr2.2:15327425-15327444 MS.gene059870:intron 45.0%
!! GGTAGCGACGAAAGTATCAA+CGG + chr2.2:15326587-15326606 MS.gene059870:CDS 45.0%
!! TTCTTGGTGCTATGTAGGGA+TGG + chr2.2:15327742-15327761 MS.gene059870:intron 45.0%
!!! GAAGGTTTTTCGAGTGCTTC+GGG + chr2.2:15326768-15326787 MS.gene059870:CDS 45.0%
!!! GGAAGGTTTTTCGAGTGCTT+CGG + chr2.2:15326767-15326786 MS.gene059870:CDS 45.0%
!!! TTTTTCGAGTGCTTCGGGTT+TGG + chr2.2:15326773-15326792 MS.gene059870:CDS 45.0%
AACCCCGCTAATCACCATCT+TGG - chr2.2:15327850-15327869 None:intergenic 50.0%
GATCCCTTGGCCAATGCATA+TGG + chr2.2:15327438-15327457 MS.gene059870:intron 50.0%
TGCCAAGATGGTGATTAGCG+GGG + chr2.2:15327845-15327864 MS.gene059870:CDS 50.0%
TGTAGGAGACTGGATTGCAC+AGG + chr2.2:15327878-15327897 MS.gene059870:CDS 50.0%
! GAGGAAAGTGTGGTGAGGTA+CGG - chr2.2:15326469-15326488 None:intergenic 50.0%
!! AGCGACGAAAGTATCAACGG+AGG + chr2.2:15326590-15326609 MS.gene059870:CDS 50.0%
!! AGGTTTGATAGTGAGCCGTG+TGG + chr2.2:15326662-15326681 MS.gene059870:CDS 50.0%
CTCCACCTTCCATCTCCACA+CGG - chr2.2:15326680-15326699 None:intergenic 55.0%
GATAGTGAGCCGTGTGGAGA+TGG + chr2.2:15326668-15326687 MS.gene059870:CDS 55.0%
GTGACGACGACGCAAGTGAA+TGG + chr2.2:15326714-15326733 MS.gene059870:CDS 55.0%
GTGGAGATGGAAGGTGGAGA+TGG + chr2.2:15326681-15326700 MS.gene059870:CDS 55.0%
! GAAGGGAGGAAAGTGTGGTG+AGG - chr2.2:15326474-15326493 None:intergenic 55.0%
! TCGAGTGCTTCGGGTTTGGT+TGG + chr2.2:15326777-15326796 MS.gene059870:CDS 55.0%
!! GTGAGGTACGGTGCTGTGAA+CGG - chr2.2:15326457-15326476 None:intergenic 55.0%
AGCCGTGTGGAGATGGAAGG+TGG + chr2.2:15326675-15326694 MS.gene059870:CDS 60.0%
CCACTCGCGTCACACCAAGT+TGG - chr2.2:15327400-15327419 None:intergenic 60.0%
GTGAGCCGTGTGGAGATGGA+AGG + chr2.2:15326672-15326691 MS.gene059870:CDS 60.0%
!! CCAACTTGGTGTGACGCGAG+TGG + chr2.2:15327397-15327416 MS.gene059870:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 15326447 15327935 15326447 ID=MS.gene059870
chr2.2 mRNA 15326447 15327935 15326447 ID=MS.gene059870.t1;Parent=MS.gene059870
chr2.2 exon 15326447 15326929 15326447 ID=MS.gene059870.t1.exon1;Parent=MS.gene059870.t1
chr2.2 CDS 15326447 15326929 15326447 ID=cds.MS.gene059870.t1;Parent=MS.gene059870.t1
chr2.2 exon 15327759 15327935 15327759 ID=MS.gene059870.t1.exon2;Parent=MS.gene059870.t1
chr2.2 CDS 15327759 15327935 15327759 ID=cds.MS.gene059870.t1;Parent=MS.gene059870.t1
Gene Sequence

>MS.gene059870

ATGGCTTCCGTTCACAGCACCGTACCTCACCACACTTTCCTCCCTTCAATTTCCACACCAAAATCAAAATCCATTTCCAAGCCACAAAACTTTCCAGTATCAAGATTCTCCACTAATCCGAATTTCACAAAACGTGCAGCGGTAGCGACGAAAGTATCAACGGAGGAGTTCGACGTAATAGCAGTACAGAGCAACGATATTACAGACCAGCAAGAAGGTTTGATAGTGAGCCGTGTGGAGATGGAAGGTGGAGATGGTGAATTAGCGGTGACGACGACGCAAGTGAATGGATTTGGAGCGAATGAAGGTTTGTTATCGTTGGAAGGTTTTTCGAGTGCTTCGGGTTTGGTTGGTAATGAGAATGAAGAGAATGTGGAGAGTGTGGAGAAGCTTCTTGATAGAAGTATTAATGCTAGTATTGTTCTTGTTGCTGGTACTTTTGCTCTTACGAAGCTTCTTACTATTGATAGTGATTATTGGCATGTAAGTTTAGAACAACTCAATCGTGCTTATCTGAAATCGTGTGTGTGTGTGTGTGATTCTTTGGATTTGGAAATGATTAAATTTGGTTGTACTGTCGAAATTTTGATACAAAATTTGCAATTTTATAGACTAGGCCAGGTTTGGATTGACTTATTTTTTAGCTTATGAAAGATAGTTTATGCAAATAAATAATCCTTTACGTTATGTTTTATTTTATTTTATGACAGCATAATTTTTTATGTTAATTCTTAAGTTCATGCTAATGAAAATTGTATTTTATAAGCTGTTTTTCATAAACTACCTTGACAAGCTTATGAATAATACATAAAAGCTTATTTATTTGCATAACCAGTTTTGCATATGCTAAAAACTAAGCCAATCCAAACAGTATAGTGATTATGGCCAAGTTTGTGTCGATTTCTAGCTTGGATCAAGTAAACTTCTCATTCAAAATTAGGTCATAGTTACCAACTTGGTGTGACGCGAGTGGTAGATACTTGAGTTGATCGATCCCTTGGCCAATGCATATGGAAAAATATTTGTTTGGAGAGAGACTGATCTCTTTAATGAATCTTAGTTGTCTAGAAAAATTAGTCTATACATTTGCATGCAGGGAGGGTTTTACCAAGAAAAAGTATAGTTCTTCGTCTTGTAGAAAGAGTCCTTATAAGCTAATTTAAAGTTGGTCTAAGTTATGTGTTTATTCCAAATAAGCTAATTTAAACGTGCTCCAGTTGCTAATTTAGATTAGCCCTGTATACCCAGTCAAATTTGATTATCTTGAATTTTTCCTTATCTGCTAGGATTTGTGTTTCTTGGTGCTATGTAGGGATGGACAATCTATGAGATAGTAAGATATGCACCTCTACACAACTGGTTAGCTTATGAGGAAGCTCTCAAGACAAATCCGGTTTTTGCCAAGATGGTGATTAGCGGGGTTGTTTATTCTGTAGGAGACTGGATTGCACAGGTGAGTTTTTGTGTATTTCATTTCATATACAGCTGA

Protein sequence

>MS.gene059870.t1

MASVHSTVPHHTFLPSISTPKSKSISKPQNFPVSRFSTNPNFTKRAAVATKVSTEEFDVIAVQSNDITDQQEGLIVSRVEMEGGDGELAVTTTQVNGFGANEGLLSLEGFSSASGLVGNENEENVESVEKLLDRSINASIVLVAGTFALTKLLTIDSDYWHGWTIYEIVRYAPLHNWLAYEEALKTNPVFAKMVISGVVYSVGDWIAQVSFCVFHFIYS