Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98137.t1 | XP_013454491.1 | 99.3 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 1.20E-83 | 318.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98137.t1 | A0A072UFR3 | 99.3 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 8.9e-84 | 318.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049427 | MS.gene98137 | 0.80273 | 4.88E-49 | -1.69E-46 |
| MS.gene050670 | MS.gene98137 | 0.804602 | 2.00E-49 | -1.69E-46 |
| MS.gene050812 | MS.gene98137 | 0.802439 | 5.61E-49 | -1.69E-46 |
| MS.gene05105 | MS.gene98137 | 0.806038 | 9.99E-50 | -1.69E-46 |
| MS.gene051617 | MS.gene98137 | 0.800005 | 1.77E-48 | -1.69E-46 |
| MS.gene052310 | MS.gene98137 | 0.805141 | 1.54E-49 | -1.69E-46 |
| MS.gene052340 | MS.gene98137 | 0.809105 | 2.23E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene98137 | 0.822344 | 2.49E-53 | -1.69E-46 |
| MS.gene052484 | MS.gene98137 | 0.812887 | 3.38E-51 | -1.69E-46 |
| MS.gene053103 | MS.gene98137 | 0.816541 | 5.24E-52 | -1.69E-46 |
| MS.gene053207 | MS.gene98137 | 0.800863 | 1.18E-48 | -1.69E-46 |
| MS.gene053209 | MS.gene98137 | 0.851251 | 1.00E-60 | -1.69E-46 |
| MS.gene05553 | MS.gene98137 | 0.80043 | 1.45E-48 | -1.69E-46 |
| MS.gene056659 | MS.gene98137 | 0.812747 | 3.63E-51 | -1.69E-46 |
| MS.gene056796 | MS.gene98137 | 0.815657 | 8.26E-52 | -1.69E-46 |
| MS.gene056897 | MS.gene98137 | 0.826753 | 2.28E-54 | -1.69E-46 |
| MS.gene05702 | MS.gene98137 | 0.802451 | 5.57E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene98137 | 0.851203 | 1.03E-60 | -1.69E-46 |
| MS.gene058082 | MS.gene98137 | 0.836046 | 1.18E-56 | -1.69E-46 |
| MS.gene058233 | MS.gene98137 | 0.83112 | 2.00E-55 | -1.69E-46 |
| MS.gene058276 | MS.gene98137 | 0.819415 | 1.18E-52 | -1.69E-46 |
| MS.gene058365 | MS.gene98137 | 0.821428 | 4.06E-53 | -1.69E-46 |
| MS.gene058746 | MS.gene98137 | 0.812448 | 4.22E-51 | -1.69E-46 |
| MS.gene058748 | MS.gene98137 | 0.824291 | 8.74E-54 | -1.69E-46 |
| MS.gene059688 | MS.gene98137 | 0.82024 | 7.62E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene98137 | 0.837382 | 5.37E-57 | -1.69E-46 |
| MS.gene059695 | MS.gene98137 | 0.835586 | 1.54E-56 | -1.69E-46 |
| MS.gene059894 | MS.gene98137 | 0.807963 | 3.91E-50 | -1.69E-46 |
| MS.gene060407 | MS.gene98137 | 0.830387 | 3.02E-55 | -1.69E-46 |
| MS.gene06092 | MS.gene98137 | 0.800135 | 1.66E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene98137 | 0.802535 | 5.36E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98137.t1 | MTR_4g005360 | 99.338 | 151 | 1 | 0 | 1 | 151 | 83 | 233 | 7.44e-111 | 314 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98137.t1 | AT3G20270 | 57.241 | 145 | 60 | 2 | 1 | 145 | 90 | 232 | 8.53e-54 | 180 |
| MS.gene98137.t1 | AT3G20270 | 57.241 | 145 | 60 | 2 | 1 | 145 | 90 | 232 | 8.53e-54 | 180 |
Find 35 sgRNAs with CRISPR-Local
Find 60 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTTGCTGTTCTTGACAATT+TGG | 0.246299 | 4.1:+89219232 | MS.gene98137:CDS |
| ATCTCATCCACTTGTATATC+AGG | 0.333453 | 4.1:-89218767 | None:intergenic |
| ACTGATCTAGTGTAAGGATA+TGG | 0.366614 | 4.1:-89219516 | None:intergenic |
| ATGAACTTGTATCTCGAATC+TGG | 0.411354 | 4.1:+89219210 | MS.gene98137:CDS |
| CCAATACCATCTGCTTGGTT+AGG | 0.422828 | 4.1:+89219645 | MS.gene98137:CDS |
| TATTTGTTGACTTATATGTA+AGG | 0.446098 | 4.1:-89218732 | None:intergenic |
| CCTAACCAAGCAGATGGTAT+TGG | 0.447991 | 4.1:-89219645 | None:intergenic |
| ATACTTCACGGAACCATGAT+TGG | 0.450822 | 4.1:+89218857 | MS.gene98137:CDS |
| TACAAGTGGATGAGATTGTT+GGG | 0.457723 | 4.1:+89218774 | MS.gene98137:CDS |
| TGTTGGCATGTGCTAAAAGT+AGG | 0.469168 | 4.1:+89219616 | MS.gene98137:CDS |
| ACTTTGACTGATCTAGTGTA+AGG | 0.474599 | 4.1:-89219522 | None:intergenic |
| TCCACCATGAGGAGGCATAA+TGG | 0.492329 | 4.1:-89218834 | None:intergenic |
| TTCAGCCAATACCATCTGCT+TGG | 0.495555 | 4.1:+89219640 | MS.gene98137:CDS |
| GCAGATCAGTTTATTGCCAA+TGG | 0.496622 | 4.1:+89219167 | MS.gene98137:intron |
| AGATTCGAGATACAAGTTCA+TGG | 0.497424 | 4.1:-89219208 | None:intergenic |
| CTAGATCAGTCAAAGTTCAA+TGG | 0.503366 | 4.1:+89219529 | MS.gene98137:CDS |
| CCTCATGGTGGAATACTTCA+CGG | 0.510920 | 4.1:+89218845 | MS.gene98137:CDS |
| TACGTCTCTTGACTTGCCAT+TGG | 0.513346 | 4.1:-89219183 | None:intergenic |
| TGTAGATTATTATGATATGT+TGG | 0.518385 | 4.1:+89219599 | MS.gene98137:CDS |
| CTTAAACGCCTTGCACATGA+AGG | 0.518985 | 4.1:+89219281 | MS.gene98137:CDS |
| AAGGCGTTTAAGTAAGCAAA+AGG | 0.541095 | 4.1:-89219270 | None:intergenic |
| GGGGCATGCCATACCAATCA+TGG | 0.551161 | 4.1:-89218870 | None:intergenic |
| CTTTCCATTATGCCTCCTCA+TGG | 0.553623 | 4.1:+89218830 | MS.gene98137:CDS |
| ATACAAGTGGATGAGATTGT+TGG | 0.562800 | 4.1:+89218773 | MS.gene98137:CDS |
| CTACATGGTTGAAGCAATCA+CGG | 0.564717 | 4.1:-89219581 | None:intergenic |
| GATTCGAGATACAAGTTCAT+GGG | 0.566517 | 4.1:-89219207 | None:intergenic |
| GATCAGTCAAAGTTCAATGG+AGG | 0.590714 | 4.1:+89219532 | MS.gene98137:CDS |
| ACATATCATAATAATCTACA+TGG | 0.601342 | 4.1:-89219596 | None:intergenic |
| ATCAGTCAAAGTTCAATGGA+GGG | 0.611627 | 4.1:+89219533 | MS.gene98137:CDS |
| TAATAACCTAACCAAGCAGA+TGG | 0.613735 | 4.1:-89219651 | None:intergenic |
| TTACATCTCCTTCATGTGCA+AGG | 0.614326 | 4.1:-89219289 | None:intergenic |
| TCCATTATGCCTCCTCATGG+TGG | 0.618370 | 4.1:+89218833 | MS.gene98137:CDS |
| TTCTGATCCTGATATACAAG+TGG | 0.625145 | 4.1:+89218760 | MS.gene98137:CDS |
| TGAAGTATTCCACCATGAGG+AGG | 0.648479 | 4.1:-89218842 | None:intergenic |
| CCGTGAAGTATTCCACCATG+AGG | 0.714100 | 4.1:-89218845 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACATATCATAATAATCTACA+TGG | - | chr4.1:89219599-89219618 | None:intergenic | 20.0% |
| !! | TGTAGATTATTATGATATGT+TGG | + | chr4.1:89219599-89219618 | MS.gene98137:CDS | 20.0% |
| !! | TTCATTTCAATTAGAATCAT+AGG | - | chr4.1:89219040-89219059 | None:intergenic | 20.0% |
| !!! | TATTTGTTGACTTATATGTA+AGG | - | chr4.1:89218735-89218754 | None:intergenic | 20.0% |
| ! | ATTTCTTAGATTTGTGCAAA+AGG | + | chr4.1:89219384-89219403 | MS.gene98137:intron | 25.0% |
| ! | TGTAACAACAAAAGTAAAGA+AGG | - | chr4.1:89218897-89218916 | None:intergenic | 25.0% |
| !! | AATGAATTAGTTCTCTAAGT+TGG | + | chr4.1:89219054-89219073 | MS.gene98137:intron | 25.0% |
| !! | CTTATATCTTTATGTCCTTT+TGG | + | chr4.1:89218917-89218936 | MS.gene98137:intron | 25.0% |
| AAAGAGACAAAATGTTGATG+AGG | - | chr4.1:89219452-89219471 | None:intergenic | 30.0% | |
| AACAACAAAAGTAAAGAAGG+TGG | - | chr4.1:89218894-89218913 | None:intergenic | 30.0% | |
| ACAACAAAAGTAAAGAAGGT+GGG | - | chr4.1:89218893-89218912 | None:intergenic | 30.0% | |
| AGGATCAATTCAGTTTGATA+TGG | - | chr4.1:89219020-89219039 | None:intergenic | 30.0% | |
| TTGGTTTATCTTGTTCTTGT+TGG | + | chr4.1:89219073-89219092 | MS.gene98137:intron | 30.0% | |
| ! | TTTTTGCAATAACTTTCAGC+AGG | + | chr4.1:89219485-89219504 | MS.gene98137:intron | 30.0% |
| ACTCGACATAAGAGTTGTTA+CGG | - | chr4.1:89218949-89218968 | None:intergenic | 35.0% | |
| ACTGATCTAGTGTAAGGATA+TGG | - | chr4.1:89219519-89219538 | None:intergenic | 35.0% | |
| ACTTTGACTGATCTAGTGTA+AGG | - | chr4.1:89219525-89219544 | None:intergenic | 35.0% | |
| AGATTCGAGATACAAGTTCA+TGG | - | chr4.1:89219211-89219230 | None:intergenic | 35.0% | |
| ATACAAGTGGATGAGATTGT+TGG | + | chr4.1:89218773-89218792 | MS.gene98137:CDS | 35.0% | |
| ATCAGTCAAAGTTCAATGGA+GGG | + | chr4.1:89219533-89219552 | MS.gene98137:CDS | 35.0% | |
| ATCTCATCCACTTGTATATC+AGG | - | chr4.1:89218770-89218789 | None:intergenic | 35.0% | |
| ATGAACTTGTATCTCGAATC+TGG | + | chr4.1:89219210-89219229 | MS.gene98137:CDS | 35.0% | |
| CAAAAGGATGAGATCTTTAC+TGG | + | chr4.1:89219400-89219419 | MS.gene98137:intron | 35.0% | |
| CAACAAAAGTAAAGAAGGTG+GGG | - | chr4.1:89218892-89218911 | None:intergenic | 35.0% | |
| CTAGATCAGTCAAAGTTCAA+TGG | + | chr4.1:89219529-89219548 | MS.gene98137:CDS | 35.0% | |
| GATTCGAGATACAAGTTCAT+GGG | - | chr4.1:89219210-89219229 | None:intergenic | 35.0% | |
| TAATAACCTAACCAAGCAGA+TGG | - | chr4.1:89219654-89219673 | None:intergenic | 35.0% | |
| TACAAGTGGATGAGATTGTT+GGG | + | chr4.1:89218774-89218793 | MS.gene98137:CDS | 35.0% | |
| TTCTGATCCTGATATACAAG+TGG | + | chr4.1:89218760-89218779 | MS.gene98137:CDS | 35.0% | |
| TTGTAGGAGAGAGGTTAATA+TGG | + | chr4.1:89218970-89218989 | MS.gene98137:intron | 35.0% | |
| TTGTTACGGGATAAACCAAA+AGG | - | chr4.1:89218935-89218954 | None:intergenic | 35.0% | |
| ! | AAGGCGTTTAAGTAAGCAAA+AGG | - | chr4.1:89219273-89219292 | None:intergenic | 35.0% |
| !! | ACTCTTATGTCGAGTTTTGT+AGG | + | chr4.1:89218954-89218973 | MS.gene98137:intron | 35.0% |
| ATACTTCACGGAACCATGAT+TGG | + | chr4.1:89218857-89218876 | MS.gene98137:CDS | 40.0% | |
| CTACATGGTTGAAGCAATCA+CGG | - | chr4.1:89219584-89219603 | None:intergenic | 40.0% | |
| CTCGACATAAGAGTTGTTAC+GGG | - | chr4.1:89218948-89218967 | None:intergenic | 40.0% | |
| GATCAGTCAAAGTTCAATGG+AGG | + | chr4.1:89219532-89219551 | MS.gene98137:CDS | 40.0% | |
| GCAGATCAGTTTATTGCCAA+TGG | + | chr4.1:89219167-89219186 | MS.gene98137:intron | 40.0% | |
| TGTTGGCATGTGCTAAAAGT+AGG | + | chr4.1:89219616-89219635 | MS.gene98137:CDS | 40.0% | |
| TTACATCTCCTTCATGTGCA+AGG | - | chr4.1:89219292-89219311 | None:intergenic | 40.0% | |
| !! | GCTTGCTGTTCTTGACAATT+TGG | + | chr4.1:89219232-89219251 | MS.gene98137:CDS | 40.0% |
| CCAATACCATCTGCTTGGTT+AGG | + | chr4.1:89219645-89219664 | MS.gene98137:CDS | 45.0% | |
| CCTAACCAAGCAGATGGTAT+TGG | - | chr4.1:89219648-89219667 | None:intergenic | 45.0% | |
| CTTAAACGCCTTGCACATGA+AGG | + | chr4.1:89219281-89219300 | MS.gene98137:CDS | 45.0% | |
| CTTTCCATTATGCCTCCTCA+TGG | + | chr4.1:89218830-89218849 | MS.gene98137:CDS | 45.0% | |
| TCACGGAACCATGATTGGTA+TGG | + | chr4.1:89218862-89218881 | MS.gene98137:intron | 45.0% | |
| TTCAGCCAATACCATCTGCT+TGG | + | chr4.1:89219640-89219659 | MS.gene98137:CDS | 45.0% | |
| ! | CCTCATGGTGGAATACTTCA+CGG | + | chr4.1:89218845-89218864 | MS.gene98137:CDS | 45.0% |
| ! | TGAAGTATTCCACCATGAGG+AGG | - | chr4.1:89218845-89218864 | None:intergenic | 45.0% |
| !! | TACGTCTCTTGACTTGCCAT+TGG | - | chr4.1:89219186-89219205 | None:intergenic | 45.0% |
| !! | TGTCGAGTTTTGTAGGAGAG+AGG | + | chr4.1:89218961-89218980 | MS.gene98137:intron | 45.0% |
| !! | AAATAATGATAAAAATATAT+AGG | - | chr4.1:89219368-89219387 | None:intergenic | 5.0% |
| CGCCCATAATCTAGCAAAGG+CGG | + | chr4.1:89218994-89219013 | MS.gene98137:intron | 50.0% | |
| GGCCGCCTTTGCTAGATTAT+GGG | - | chr4.1:89218999-89219018 | None:intergenic | 50.0% | |
| GGTCGCCCATAATCTAGCAA+AGG | + | chr4.1:89218991-89219010 | MS.gene98137:intron | 50.0% | |
| TCCATTATGCCTCCTCATGG+TGG | + | chr4.1:89218833-89218852 | MS.gene98137:CDS | 50.0% | |
| TGGCCGCCTTTGCTAGATTA+TGG | - | chr4.1:89219000-89219019 | None:intergenic | 50.0% | |
| ! | CCGTGAAGTATTCCACCATG+AGG | - | chr4.1:89218848-89218867 | None:intergenic | 50.0% |
| ! | TCCACCATGAGGAGGCATAA+TGG | - | chr4.1:89218837-89218856 | None:intergenic | 50.0% |
| GGGGCATGCCATACCAATCA+TGG | - | chr4.1:89218873-89218892 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 89218728 | 89219674 | 89218728 | ID=MS.gene98137 |
| chr4.1 | mRNA | 89218728 | 89219674 | 89218728 | ID=MS.gene98137.t1;Parent=MS.gene98137 |
| chr4.1 | exon | 89218728 | 89218878 | 89218728 | ID=MS.gene98137.t1.exon1;Parent=MS.gene98137.t1 |
| chr4.1 | CDS | 89218728 | 89218878 | 89218728 | ID=cds.MS.gene98137.t1;Parent=MS.gene98137.t1 |
| chr4.1 | exon | 89219171 | 89219307 | 89219171 | ID=MS.gene98137.t1.exon2;Parent=MS.gene98137.t1 |
| chr4.1 | CDS | 89219171 | 89219307 | 89219171 | ID=cds.MS.gene98137.t1;Parent=MS.gene98137.t1 |
| chr4.1 | exon | 89219507 | 89219674 | 89219507 | ID=MS.gene98137.t1.exon3;Parent=MS.gene98137.t1 |
| chr4.1 | CDS | 89219507 | 89219674 | 89219507 | ID=cds.MS.gene98137.t1;Parent=MS.gene98137.t1 |
| Gene Sequence |
| Protein sequence |
