AlfalfaGEDB | MsG0180005780.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005780.01

KEGG: -
GO: -
PFAM: PF02704

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005780.01.T01	XP_013470003.1	98.889	90	1	0	1	90	1	90	2.20E-56	179

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005780.01.T01	Q948Z4	71.233	73	19	1	18	90	18	88	5.51E-29	102

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005780.01.T01	A0A072VQU0	98.889	90	1	0	1	90	1	90	1.05e-56	179

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048738.01	MsG0180005780.01	0.816343	5.806786e-52	4.104179e-49
MsG0180001169.01	MsG0180005780.01	0.810982	8.791792e-51	5.377697e-48
MsG0180003905.01	MsG0180005780.01	0.864569	1.106276e-64	3.489204e-61
MsG0180004064.01	MsG0180005780.01	0.826787	2.239958e-54	2.121653e-51
MsG0180004652.01	MsG0180005780.01	0.800148	1.650967e-48	7.630866e-46
MsG0180005780.01	MsG0280006717.01	0.812761	3.603277e-51	2.310542e-48
MsG0180005780.01	MsG0380015402.01	0.850132	2.069199e-60	4.012512e-57
MsG0180005780.01	MsG0380016031.01	0.817389	3.383273e-52	2.460625e-49
MsG0180005780.01	MsG0380017588.01	0.856781	2.550512e-62	6.155946e-59
MsG0180005780.01	MsG0380017826.01	0.810495	1.120652e-50	6.765261e-48
MsG0180005780.01	MsG0480018228.01	0.800525	1.383671e-48	6.456881e-46
MsG0180005780.01	MsG0480020398.01	0.809396	1.931635e-50	1.132889e-47
MsG0180005780.01	MsG0480021301.01	0.807493	4.919432e-50	2.744081e-47
MsG0180005780.01	MsG0480022302.01	0.802117	6.531008e-49	3.173104e-46
MsG0180005780.01	MsG0480023626.01	0.810741	9.913136e-51	6.024172e-48
MsG0180005780.01	MsG0480023663.01	0.809337	1.988741e-50	1.164539e-47
MsG0180005780.01	MsG0480023706.01	0.820760	5.783752e-53	4.617570e-50
MsG0180005780.01	MsG0480023913.01	0.803026	4.243256e-49	2.109802e-46
MsG0180005780.01	MsG0580026029.01	0.815390	9.476884e-52	6.525137e-49
MsG0180005780.01	MsG0580026343.01	0.846653	1.897909e-59	3.289735e-56
MsG0180005780.01	MsG0580026446.01	0.800052	1.726936e-48	7.962583e-46
MsG0180005780.01	MsG0680031006.01	0.801192	1.010874e-48	4.797824e-46
MsG0180005780.01	MsG0780038647.01	0.816303	5.927973e-52	4.185243e-49
MsG0180005780.01	MsG0780040978.01	0.860309	2.260512e-63	6.151413e-60
MsG0180005780.01	MsG0780040990.01	0.831541	1.574278e-55	1.713762e-52
MsG0180005780.01	MsG0780041591.01	0.817286	3.568138e-52	2.587629e-49
MsG0180005780.01	MsG0880042833.01	0.826338	2.865563e-54	2.679811e-51
MsG0180005780.01	MsG0880044356.01	0.800450	1.432940e-48	6.674143e-46
MsG0180005780.01	MsG0880045356.01	0.828720	7.684560e-55	7.700583e-52

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005780.01.T01	MTR_1g106915	98.889	90	1	0	1	90	1	90	2.66e-60	179
MsG0180005780.01.T01	MTR_1g018640	64.835	91	31	1	1	90	1	91	3.61e-30	102
MsG0180005780.01.T01	MTR_3g102660	67.692	65	21	0	26	90	21	85	5.28e-26	92.0
MsG0180005780.01.T01	MTR_5g036860	46.237	93	46	2	1	90	1	92	1.68e-20	78.6
MsG0180005780.01.T01	MTR_5g036640	47.561	82	41	1	9	90	4	83	1.83e-20	78.2
MsG0180005780.01.T01	MTR_5g036720	40.860	93	51	2	1	90	1	92	3.42e-17	70.1
MsG0180005780.01.T01	MTR_5g036880	41.935	93	50	2	1	90	1	92	6.87e-17	69.3
MsG0180005780.01.T01	MTR_5g095990	52.459	61	29	0	30	90	89	149	3.87e-16	68.9
MsG0180005780.01.T01	MTR_3g007900	52.459	61	29	0	30	90	94	154	4.26e-16	68.9
MsG0180005780.01.T01	MTR_1g096370	39.362	94	50	1	4	90	2	95	2.34e-15	65.5
MsG0180005780.01.T01	MTR_6g012360	34.951	103	48	2	7	90	5	107	7.83e-15	64.3
MsG0180005780.01.T01	MTR_1g025220	35.354	99	51	1	5	90	3	101	2.36e-14	63.2
MsG0180005780.01.T01	MTR_7g010580	46.774	62	33	0	29	90	48	109	2.65e-14	63.2
MsG0180005780.01.T01	MTR_3g037750	33.696	92	46	1	14	90	12	103	4.36e-14	62.4
MsG0180005780.01.T01	MTR_3g113890	32.653	98	51	1	8	90	6	103	1.93e-13	60.8
MsG0180005780.01.T01	MTR_7g090590	42.857	63	36	0	28	90	58	120	2.20e-13	60.8
MsG0180005780.01.T01	MTR_8g062800	45.902	61	32	1	31	90	52	112	2.25e-12	58.2
MsG0180005780.01.T01	MTR_1g025250	40.000	75	40	1	21	90	26	100	3.74e-12	57.4
MsG0180005780.01.T01	MTR_8g085470	45.679	81	41	1	13	90	15	95	7.43e-12	56.6
MsG0180005780.01.T01	MTR_6g007897	47.541	61	31	1	31	90	188	248	8.18e-12	58.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005780.01.T01	AT5G59845	60.440	91	33	2	1	90	1	89	3.97e-28	97.8
MsG0180005780.01.T01	AT2G39540	54.444	90	38	1	1	90	1	87	1.52e-26	93.6
MsG0180005780.01.T01	AT2G14900	69.492	59	18	0	32	90	50	108	4.46e-26	93.2
MsG0180005780.01.T01	AT1G10588	56.180	89	36	2	3	90	4	90	1.51e-25	91.3
MsG0180005780.01.T01	AT1G10588	53.933	89	37	2	3	90	4	89	3.50e-24	87.8
MsG0180005780.01.T01	AT1G74670	36.634	101	53	1	1	90	1	101	2.02e-16	68.2
MsG0180005780.01.T01	AT5G15230	30.189	106	58	1	1	90	1	106	2.61e-13	60.5
MsG0180005780.01.T01	AT4G09600	40.244	82	40	2	18	90	18	99	8.03e-13	58.9
MsG0180005780.01.T01	AT5G15230	44.262	61	34	0	30	90	23	83	4.61e-12	56.6
MsG0180005780.01.T01	AT1G22690	45.000	60	33	0	31	90	60	119	9.32e-11	54.3
MsG0180005780.01.T01	AT1G22690	45.000	60	33	0	31	90	60	119	9.32e-11	54.3
MsG0180005780.01.T01	AT1G22690	45.000	60	33	0	31	90	60	119	9.32e-11	54.3

Find 24 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTCTATGCTTCTCTTTCT+TGG	0.113371	1:+96098635	MsG0180005780.01.T01:CDS
CAGAGACAAGGTTAACAATA+AGG	0.287897	1:+96098943	MsG0180005780.01.T01:CDS
TGTGTTCCTTCTGGGACTTA+TGG	0.316342	1:+96098896	MsG0180005780.01.T01:CDS
GTGTTCCTTCTGGGACTTAT+GGG	0.319469	1:+96098897	MsG0180005780.01.T01:CDS
TAATTGACATCAAGGGCATT+TGG	0.329972	1:-96098972	None:intergenic
GAGTGCAAATGTGTTCCTTC+TGG	0.347493	1:+96098887	MsG0180005780.01.T01:CDS
AATTGACATCAAGGGCATTT+GGG	0.384143	1:-96098971	None:intergenic
CGAACACTTGCTGCTACAAT+TGG	0.398537	1:-96098801	None:intergenic
CTAAAGCAGGGGTTCAAGAT+AGG	0.424996	1:+96098834	MsG0180005780.01.T01:CDS
GGAGCTAGCCATTGCATATG+AGG	0.425249	1:+96098656	MsG0180005780.01.T01:CDS
AGAGACAAGGTTAACAATAA+GGG	0.465278	1:+96098944	MsG0180005780.01.T01:CDS
AGAAAGAGAAGCATAGAAGA+AGG	0.472112	1:-96098632	None:intergenic
AGTGCAAATGTGTTCCTTCT+GGG	0.495711	1:+96098888	MsG0180005780.01.T01:CDS
GAAAGAGAAGCATAGAAGAA+GGG	0.513355	1:-96098631	None:intergenic
CAATTGTAGCAGCAAGTGTT+CGG	0.519914	1:+96098802	MsG0180005780.01.T01:CDS
TCGGACAGATGCTCTAAAGC+AGG	0.531159	1:+96098821	MsG0180005780.01.T01:CDS
CGGACAGATGCTCTAAAGCA+GGG	0.574198	1:+96098822	MsG0180005780.01.T01:CDS
TTAACCTTGTCTCTGTAGCA+AGG	0.582422	1:-96098935	None:intergenic
ACATGAATCCTCATATGCAA+TGG	0.604589	1:-96098664	None:intergenic
AATAGCAACATGGTGAAGAG+AGG	0.630987	1:-96098603	None:intergenic
TTGTTCCCATAAGTCCCAGA+AGG	0.664123	1:-96098902	None:intergenic
ATGTCCTTGCTACAGAGACA+AGG	0.668753	1:+96098931	MsG0180005780.01.T01:CDS
GGACAGATGCTCTAAAGCAG+GGG	0.697192	1:+96098823	MsG0180005780.01.T01:CDS
AAGGGAACAGAATAGCAACA+TGG	0.736288	1:-96098613	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATATTCTTTTACTTTTTT+TGG	+	Chr1:96098737-96098756	MsG0180005780.01.T01:intron	10.0%
!!!	TGCATTTCCACTTTTAATAA+TGG	+	Chr1:96098775-96098794	MsG0180005780.01.T01:intron	25.0%
	AGAGACAAGGTTAACAATAA+GGG	+	Chr1:96098944-96098963	MsG0180005780.01.T01:CDS	30.0%
	GAATCCTCTCATTTCATAAT+AGG	-	Chr1:96098702-96098721	None:intergenic	30.0%
	TTCACCTATTATGAAATGAG+AGG	+	Chr1:96098695-96098714	MsG0180005780.01.T01:intron	30.0%
	ACATGAATCCTCATATGCAA+TGG	-	Chr1:96098667-96098686	None:intergenic	35.0%
	CAGAGACAAGGTTAACAATA+AGG	+	Chr1:96098943-96098962	MsG0180005780.01.T01:CDS	35.0%
	TCTTCTATGCTTCTCTTTCT+TGG	+	Chr1:96098635-96098654	MsG0180005780.01.T01:CDS	35.0%
	TTGGCTGCCATTATTAAAAG+TGG	-	Chr1:96098785-96098804	None:intergenic	35.0%
!	AGAAAGAGAAGCATAGAAGA+AGG	-	Chr1:96098635-96098654	None:intergenic	35.0%
!	GAAAGAGAAGCATAGAAGAA+GGG	-	Chr1:96098634-96098653	None:intergenic	35.0%
	AAGGGAACAGAATAGCAACA+TGG	-	Chr1:96098616-96098635	None:intergenic	40.0%
	AATAGCAACATGGTGAAGAG+AGG	-	Chr1:96098606-96098625	None:intergenic	40.0%
	AGTGCAAATGTGTTCCTTCT+GGG	+	Chr1:96098888-96098907	MsG0180005780.01.T01:CDS	40.0%
	CAATTGTAGCAGCAAGTGTT+CGG	+	Chr1:96098802-96098821	MsG0180005780.01.T01:CDS	40.0%
	TTAACCTTGTCTCTGTAGCA+AGG	-	Chr1:96098938-96098957	None:intergenic	40.0%
	ATGTCCTTGCTACAGAGACA+AGG	+	Chr1:96098931-96098950	MsG0180005780.01.T01:CDS	45.0%
	CTAAAGCAGGGGTTCAAGAT+AGG	+	Chr1:96098834-96098853	MsG0180005780.01.T01:CDS	45.0%
	GAGTGCAAATGTGTTCCTTC+TGG	+	Chr1:96098887-96098906	MsG0180005780.01.T01:CDS	45.0%
	TTGTTCCCATAAGTCCCAGA+AGG	-	Chr1:96098905-96098924	None:intergenic	45.0%
!	CGAACACTTGCTGCTACAAT+TGG	-	Chr1:96098804-96098823	None:intergenic	45.0%
!!	GTGTTCCTTCTGGGACTTAT+GGG	+	Chr1:96098897-96098916	MsG0180005780.01.T01:CDS	45.0%
!!	TGTGTTCCTTCTGGGACTTA+TGG	+	Chr1:96098896-96098915	MsG0180005780.01.T01:CDS	45.0%
	GGAGCTAGCCATTGCATATG+AGG	+	Chr1:96098656-96098675	MsG0180005780.01.T01:CDS	50.0%
!!	CGGACAGATGCTCTAAAGCA+GGG	+	Chr1:96098822-96098841	MsG0180005780.01.T01:CDS	50.0%
!!	GGACAGATGCTCTAAAGCAG+GGG	+	Chr1:96098823-96098842	MsG0180005780.01.T01:CDS	50.0%
!!	TCGGACAGATGCTCTAAAGC+AGG	+	Chr1:96098821-96098840	MsG0180005780.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	96098597	96098985	96098597	ID=MsG0180005780.01;Name=MsG0180005780.01
Chr1	mRNA	96098597	96098985	96098597	ID=MsG0180005780.01.T01;Parent=MsG0180005780.01;Name=MsG0180005780.01.T01;_AED=0.50;_eAED=0.51;_QI=0\|0\|0\|1\|0\|0\|2\|0\|90
Chr1	exon	96098597	96098684	96098597	ID=MsG0180005780.01.T01:exon:10580;Parent=MsG0180005780.01.T01
Chr1	exon	96098801	96098985	96098801	ID=MsG0180005780.01.T01:exon:10581;Parent=MsG0180005780.01.T01
Chr1	CDS	96098597	96098684	96098597	ID=MsG0180005780.01.T01:cds;Parent=MsG0180005780.01.T01
Chr1	CDS	96098801	96098985	96098801	ID=MsG0180005780.01.T01:cds;Parent=MsG0180005780.01.T01

Gene Sequence	>MsG0180005780.01.T01 ATGAAGCCTCTCTTCACCATGTTGCTATTCTGTTCCCTTCTTCTATGCTTCTCTTTCTTGGAGCTAGCCATTGCATATGAGGATTCATCCAATTGTAGCAGCAAGTGTTCGGACAGATGCTCTAAAGCAGGGGTTCAAGATAGGTGTGTGAAATACTGTGAAATATGCTGCAATGAGTGCAAATGTGTTCCTTCTGGGACTTATGGGAACAAGCATGAATGTCCTTGCTACAGAGACAAGGTTAACAATAAGGGAAAGCCCAAATGCCCTTGA
Protein sequence	>MsG0180005780.01.T01 MKPLFTMLLFCSLLLCFSFLELAIAYEDSSNCSSKCSDRCSKAGVQDRCVKYCEICCNECKCVPSGTYGNKHECPCYRDKVNNKGKPKCP*