AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041591.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041591.01.T02 MTR_7g114020 92.045 88 3 1 1 88 1 84 5.73e-51 157
MsG0780041591.01.T02 MTR_1g082290 67.045 88 28 1 1 88 1 87 9.24e-35 116
MsG0780041591.01.T02 MTR_1g089130 51.807 83 39 1 6 88 3 84 1.03e-26 95.9
MsG0780041591.01.T02 MTR_2g020770 60.000 85 33 1 4 88 3 86 1.22e-23 87.8
MsG0780041591.01.T02 MTR_2g020770 60.000 85 33 1 4 88 3 86 1.76e-23 87.8
MsG0780041591.01.T02 MTR_2g100880 46.914 81 42 1 8 88 7 86 1.53e-21 82.8
MsG0780041591.01.T02 MTR_2g100900 46.914 81 42 1 8 88 7 86 1.61e-21 82.4
MsG0780041591.01.T01 MTR_7g114020 89.908 109 7 1 1 109 1 105 1.78e-64 192
MsG0780041591.01.T01 MTR_1g082290 67.308 104 33 1 1 104 1 103 3.06e-45 144
MsG0780041591.01.T01 MTR_1g089130 52.525 99 46 1 6 104 3 100 1.20e-34 117
MsG0780041591.01.T01 MTR_2g020770 61.386 101 38 1 4 104 3 102 3.37e-33 113
MsG0780041591.01.T01 MTR_2g020770 61.386 101 38 1 4 104 3 102 4.74e-33 112
MsG0780041591.01.T01 MTR_2g100880 48.315 89 45 1 8 96 7 94 7.48e-26 94.4
MsG0780041591.01.T01 MTR_2g100900 48.315 89 45 1 8 96 7 94 7.65e-26 94.4
MsG0780041591.01.T01 MTR_2g103870 48.837 86 44 0 18 103 20 105 1.49e-18 75.9
MsG0780041591.01.T01 MTR_4g010160 50.000 88 44 0 16 103 6 93 2.38e-15 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041591.01.T02 AT5G39340 58.824 85 35 0 4 88 5 89 2.93e-32 110
MsG0780041591.01.T02 AT5G39340 58.824 85 35 0 4 88 38 122 5.98e-32 110
MsG0780041591.01.T02 AT5G39340 58.824 85 35 0 4 88 54 138 9.31e-32 110
MsG0780041591.01.T02 AT5G39340 58.824 85 35 0 4 88 54 138 9.31e-32 110
MsG0780041591.01.T02 AT1G03430 60.000 85 34 0 4 88 6 90 6.01e-31 107
MsG0780041591.01.T02 AT1G03430 60.000 85 34 0 4 88 6 90 6.27e-31 106
MsG0780041591.01.T02 AT3G29350 55.294 85 38 0 4 88 5 89 1.01e-29 102
MsG0780041591.01.T02 AT3G29350 55.294 85 38 0 4 88 5 89 5.14e-29 102
MsG0780041591.01.T02 AT3G21510 60.000 85 33 1 4 88 3 86 2.26e-21 82.4
MsG0780041591.01.T02 AT3G16360 43.902 82 45 1 7 88 1 81 2.50e-20 79.3
MsG0780041591.01.T02 AT3G16360 44.118 68 37 1 21 88 9 75 6.84e-17 70.1
MsG0780041591.01.T02 AT4G04402 49.057 53 26 1 22 73 24 76 3.30e-12 58.9
MsG0780041591.01.T01 AT1G03430 59.223 103 42 0 4 106 6 108 8.76e-40 130
MsG0780041591.01.T01 AT1G03430 59.223 103 42 0 4 106 6 108 9.66e-40 130
MsG0780041591.01.T01 AT5G39340 55.340 103 46 0 4 106 5 107 6.36e-39 127
MsG0780041591.01.T01 AT5G39340 55.340 103 46 0 4 106 38 140 1.57e-38 127
MsG0780041591.01.T01 AT5G39340 55.340 103 46 0 4 106 54 156 2.26e-38 128
MsG0780041591.01.T01 AT5G39340 55.340 103 46 0 4 106 54 156 2.26e-38 128
MsG0780041591.01.T01 AT3G29350 52.830 106 50 0 4 109 5 110 2.75e-38 125
MsG0780041591.01.T01 AT3G29350 52.830 106 50 0 4 109 5 110 1.60e-37 124
MsG0780041591.01.T01 AT3G21510 59.406 101 40 1 4 104 3 102 5.79e-30 105
MsG0780041591.01.T01 AT3G16360 43.564 101 56 1 7 107 1 100 2.87e-27 98.2
MsG0780041591.01.T01 AT3G16360 43.678 87 48 1 21 107 9 94 7.13e-24 89.0
MsG0780041591.01.T01 AT1G80100 44.660 103 57 0 3 105 5 107 5.59e-13 61.6
MsG0780041591.01.T01 AT1G80100 44.660 103 57 0 3 105 5 107 8.16e-13 61.2
MsG0780041591.01.T01 AT4G04402 49.057 53 26 1 22 73 24 76 5.53e-12 59.3

Find 12 sgRNAs with CRISPR-Local

Find 44 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGAAGCTTCTTAATCAAT+TGG 0.405079 7:+92799900 MsG0780041591.01.T01:CDS
AGTGGTCACTCTCTTCTTTG+AGG 0.421547 7:+92799873 MsG0780041591.01.T01:CDS
ACCACTTCAACAACAAAGTC+AGG 0.424359 7:-92799856 None:intergenic
ATTAGAATTATACATTACTT+TGG 0.446513 7:-92799984 None:intergenic
CATCTTGAAGTAGTTGAAGC+TGG 0.469279 7:-92799825 None:intergenic
TGGAGCTGCTGCCTTTCAGC+TGG 0.470844 7:-92800068 None:intergenic
AGCTGAAAGGCAGCAGCTCC+AGG 0.471527 7:+92800070 MsG0780041591.01.T01:CDS
CCCTGACTTTGTTGTTGAAG+TGG 0.477305 7:+92799855 MsG0780041591.01.T01:CDS
CAAAAGTTCAATATATTACC+TGG 0.505739 7:-92800088 None:intergenic
ATTAATCCCAAGCAGAGGCC+AGG 0.527313 7:+92800005 MsG0780041591.01.T01:intron
GCTCTTGTTCACCAGCTGAA+AGG 0.590228 7:+92800057 MsG0780041591.01.T01:CDS
CCACTTCAACAACAAAGTCA+GGG 0.677112 7:-92799855 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATAACATTGATTTTAAA+AGG + Chr7:92800028-92800047 MsG0780041591.01.T01:CDS 10.0%
!!! AAATAACATTGATTTTAAAA+GGG + Chr7:92800029-92800048 MsG0780041591.01.T01:CDS 10.0%
!!! TTTTCTTTATTTTATGTATA+GGG + Chr7:92799960-92799979 MsG0780041591.01.T01:intron 10.0%
!! ATTAGAATTATACATTACTT+TGG - Chr7:92799987-92800006 None:intergenic 15.0%
!!! CTTTTCTTTATTTTATGTAT+AGG + Chr7:92799959-92799978 MsG0780041591.01.T01:intron 15.0%
!! AAAGAAAGATAGAAAGAAAA+TGG - Chr7:92799754-92799773 None:intergenic 20.0%
!!! ATACTTCTTATCTTGTTTTA+AGG - Chr7:92800246-92800265 None:intergenic 20.0%
!!! CAACTTGTATAATTTTGATA+AGG + Chr7:92800283-92800302 MsG0780041591.01.T01:intron 20.0%
!!! TAAAATCAATGTTATTTTCC+TGG - Chr7:92800026-92800045 None:intergenic 20.0%
! CAAAAGTTCAATATATTACC+TGG - Chr7:92800091-92800110 None:intergenic 25.0%
! CAATTGGAAAAAGAAATGTA+AGG + Chr7:92799916-92799935 MsG0780041591.01.T01:intron 25.0%
! TATCTTTCTTTCTTCATTTG+TGG + Chr7:92799763-92799782 MsG0780041591.01.T01:intron 25.0%
!! TCTATCTTATTTCAAGAACA+AGG - Chr7:92800191-92800210 None:intergenic 25.0%
!!! TAACATTGATTTTAAAAGGG+TGG + Chr7:92800032-92800051 MsG0780041591.01.T01:CDS 25.0%
AAATGTTACCTCTTGAAAGA+GGG - Chr7:92799705-92799724 None:intergenic 30.0%
AGAGAAGCTTCTTAATCAAT+TGG + Chr7:92799900-92799919 MsG0780041591.01.T01:CDS 30.0%
CTTATTTCAAGAACAAGGAA+AGG - Chr7:92800186-92800205 None:intergenic 30.0%
GTTATTGGAAAATACAGCAT+TGG + Chr7:92800313-92800332 MsG0780041591.01.T01:intron 30.0%
TAAATGTTACCTCTTGAAAG+AGG - Chr7:92799706-92799725 None:intergenic 30.0%
! TGTGGTGATATCTTTGAAAA+GGG + Chr7:92799781-92799800 MsG0780041591.01.T01:intron 30.0%
! TTGTGGTGATATCTTTGAAA+AGG + Chr7:92799780-92799799 MsG0780041591.01.T01:intron 30.0%
GAGAACAACAATGTATAGTG+TGG + Chr7:92799643-92799662 MsG0780041591.01.T01:intron 35.0%
GCTGGTTAAATTGATCATCT+AGG - Chr7:92799810-92799829 None:intergenic 35.0%
TGATAAGGTCGTTGAGTTAT+TGG + Chr7:92800298-92800317 MsG0780041591.01.T01:intron 35.0%
TTCTAATTAATCCCAAGCAG+AGG + Chr7:92800000-92800019 MsG0780041591.01.T01:intron 35.0%
TTGATGTGATTAAGTCAACG+AGG - Chr7:92800129-92800148 None:intergenic 35.0%
TTGCCTTCAAAAATACTGTG+AGG + Chr7:92800362-92800381 MsG0780041591.01.T01:CDS 35.0%
! TCAGTGCTACTCAATATCTT+AGG - Chr7:92799618-92799637 None:intergenic 35.0%
!! TCTCCTCACAGTATTTTTGA+AGG - Chr7:92800368-92800387 None:intergenic 35.0%
ACCACTTCAACAACAAAGTC+AGG - Chr7:92799859-92799878 None:intergenic 40.0%
CAATTCTTCCCTCTTTCAAG+AGG + Chr7:92799694-92799713 MsG0780041591.01.T01:CDS 40.0%
CATCTTGAAGTAGTTGAAGC+TGG - Chr7:92799828-92799847 None:intergenic 40.0%
CCACTTCAACAACAAAGTCA+GGG - Chr7:92799858-92799877 None:intergenic 40.0%
TGTGATTAAGTCAACGAGGT+CGG - Chr7:92800125-92800144 None:intergenic 40.0%
! GTGCTACTCAATATCTTAGG+AGG - Chr7:92799615-92799634 None:intergenic 40.0%
AGTGGTCACTCTCTTCTTTG+AGG + Chr7:92799873-92799892 MsG0780041591.01.T01:CDS 45.0%
! TATTTTCCTGGCCTCTGCTT+GGG - Chr7:92800014-92800033 None:intergenic 45.0%
! TTATTTTCCTGGCCTCTGCT+TGG - Chr7:92800015-92800034 None:intergenic 45.0%
!! CCCTGACTTTGTTGTTGAAG+TGG + Chr7:92799855-92799874 MsG0780041591.01.T01:CDS 45.0%
ATTAATCCCAAGCAGAGGCC+AGG + Chr7:92800005-92800024 MsG0780041591.01.T01:intron 50.0%
GCTCTTGTTCACCAGCTGAA+AGG + Chr7:92800057-92800076 MsG0780041591.01.T01:CDS 50.0%
GGCTCAGTTGCAAAGACAGT+TGG + Chr7:92799664-92799683 MsG0780041591.01.T01:CDS 50.0%
AGCTGAAAGGCAGCAGCTCC+AGG + Chr7:92800070-92800089 MsG0780041591.01.T01:CDS 60.0%
TGGAGCTGCTGCCTTTCAGC+TGG - Chr7:92800071-92800090 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr7 gene 92799611 92800396 92799611 ID=MsG0780041591.01;Name=MsG0780041591.01
Chr7 mRNA 92799611 92800396 92799611 ID=MsG0780041591.01.T01;Parent=MsG0780041591.01;Name=MsG0780041591.01.T01;_AED=0.41;_eAED=0.41;_QI=42|1|1|1|0|0|6|0|110
Chr7 exon 92800020 92800091 92800020 ID=MsG0780041591.01.T01:exon:21991;Parent=MsG0780041591.01.T01
Chr7 exon 92800330 92800396 92800330 ID=MsG0780041591.01.T01:exon:21992;Parent=MsG0780041591.01.T01
Chr7 five_prime_UTR 92799611 92799652 92799611 ID=MsG0780041591.01.T01:five_prime_utr;Parent=MsG0780041591.01.T01
Chr7 CDS 92799653 92799715 92799653 ID=MsG0780041591.01.T01:cds;Parent=MsG0780041591.01.T01
Chr7 CDS 92799802 92799932 92799802 ID=MsG0780041591.01.T01:cds;Parent=MsG0780041591.01.T01
Chr7 CDS 92800020 92800091 92800020 ID=MsG0780041591.01.T01:cds;Parent=MsG0780041591.01.T01
Chr7 CDS 92800330 92800396 92800330 ID=MsG0780041591.01.T01:cds;Parent=MsG0780041591.01.T01
Chr7 mRNA 92799611 92800095 92799611 ID=MsG0780041591.01.T02;Parent=MsG0780041591.01;Name=MsG0780041591.01.T02;_AED=0.46;_eAED=0.46;_QI=42|1|0.66|1|0|0|3|0|89
Chr7 exon 92800020 92800095 92800020 ID=MsG0780041591.01.T02:exon:21993;Parent=MsG0780041591.01.T02
Chr7 five_prime_UTR 92799611 92799652 92799611 ID=MsG0780041591.01.T02:five_prime_utr;Parent=MsG0780041591.01.T02
Chr7 CDS 92799653 92799715 92799653 ID=MsG0780041591.01.T02:cds;Parent=MsG0780041591.01.T02
Chr7 CDS 92799802 92799932 92799802 ID=MsG0780041591.01.T02:cds;Parent=MsG0780041591.01.T02
Chr7 CDS 92800020 92800095 92800020 ID=MsG0780041591.01.T02:cds;Parent=MsG0780041591.01.T02
Chr7 mRNA 92799812 92800009 92799812 ID=MsG0780041591.01.T03;Parent=MsG0780041591.01;Name=MsG0780041591.01.T03;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|0|1|0|65
Chr7 exon 92799812 92800009 92799812 ID=MsG0780041591.01.T03:exon:21994;Parent=MsG0780041591.01.T03
Chr7 CDS 92799812 92800009 92799812 ID=MsG0780041591.01.T03:cds;Parent=MsG0780041591.01.T03
Gene Sequence

>MsG0780041591.01.T02

ATGTATAGTGTGGCTCAGTTGCAAAGACAGTTGGTTGAATACAATTCTTCCCTCTTTCAAGAGGGATTCCTAGATGATCAATTTAACCAGCTTCAACTACTTCAAGATGAAAGCAACCCTGACTTTGTTGTTGAAGTGGTCACTCTCTTCTTTGAGGATGCAGAGAAGCTTCTTAATCAATTGGAAAAAGAAATAGGCCAGGAAAATAACATTGATTTTAAAAGGGTGGATGCTCTTGTTCACCAGCTGAAAGGCAGCAGCTCCAGGTAA

>MsG0780041591.01.T03

ATGATCAATTTAACCAGCTTCAACTACTTCAAGATGAAAGCAACCCTGACTTTGTTGTTGAAGTGGTCACTCTCTTCTTTGAGGATGCAGAGAAGCTTCTTAATCAATTGGAAAAAGAAATGTAAGGACATATACTATAAATTCACTCTTTTCTTTATTTTATGTATAGGGACCAAAGTAATGTATAATTCTAATTAA

>MsG0780041591.01.T01

ATGTATAGTGTGGCTCAGTTGCAAAGACAGTTGGTTGAATACAATTCTTCCCTCTTTCAAGAGGGATTCCTAGATGATCAATTTAACCAGCTTCAACTACTTCAAGATGAAAGCAACCCTGACTTTGTTGTTGAAGTGGTCACTCTCTTCTTTGAGGATGCAGAGAAGCTTCTTAATCAATTGGAAAAAGAAATAGGCCAGGAAAATAACATTGATTTTAAAAGGGTGGATGCTCTTGTTCACCAGCTGAAAGGCAGCAGCTCCAGCATTGGCGCACAGAGAGTTCAGAGAGTCTGTATTGCCTTCAAAAATACTGTGAGGAGAAAAACATAG

Protein sequence

>MsG0780041591.01.T02

MYSVAQLQRQLVEYNSSLFQEGFLDDQFNQLQLLQDESNPDFVVEVVTLFFEDAEKLLNQLEKEIGQENNIDFKRVDALVHQLKGSSSR*

>MsG0780041591.01.T03

MINLTSFNYFKMKATLTLLLKWSLSSLRMQRSFLINWKKKCKDIYYKFTLFFILCIGTKVMYNSN*

>MsG0780041591.01.T01

MYSVAQLQRQLVEYNSSLFQEGFLDDQFNQLQLLQDESNPDFVVEVVTLFFEDAEKLLNQLEKEIGQENNIDFKRVDALVHQLKGSSSSIGAQRVQRVCIAFKNTVRRKT*