Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008715.01.T01 | CAA2958641.1 | 64.706 | 68 | 24 | 0 | 2 | 69 | 49 | 116 | 2.05E-22 | 94.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280008715.01.T01 | A0A6A5P522 | 69.231 | 65 | 20 | 0 | 1 | 65 | 217 | 281 | 1.96e-20 | 92.4 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048938.01 | MsG0280008715.01 | 0.805963 | 1.035598e-49 | 5.553451e-47 |
| MsG0180000582.01 | MsG0280008715.01 | 0.807007 | 6.236684e-50 | 3.435048e-47 |
| MsG0180001523.01 | MsG0280008715.01 | 0.852761 | 3.733335e-61 | 7.882820e-58 |
| MsG0180006255.01 | MsG0280008715.01 | -0.824325 | 8.581705e-54 | 7.576423e-51 |
| MsG0280008139.01 | MsG0280008715.01 | 0.818335 | 2.068456e-52 | 1.543956e-49 |
| MsG0280008715.01 | MsG0280008717.01 | 0.971757 | 1.141663e-133 | 1.274440e-127 |
| MsG0280008715.01 | MsG0380012931.01 | 0.856791 | 2.533700e-62 | 6.116954e-59 |
| MsG0280008715.01 | MsG0380013687.01 | 0.819644 | 1.042274e-52 | 8.066650e-50 |
| MsG0280008715.01 | MsG0380014646.01 | 0.811485 | 6.838843e-51 | 4.238641e-48 |
| MsG0280008715.01 | MsG0380015362.01 | 0.843280 | 1.544174e-58 | 2.404122e-55 |
| MsG0280008715.01 | MsG0380015520.01 | -0.801458 | 8.919656e-49 | 4.261792e-46 |
| MsG0280008715.01 | MsG0380015847.01 | -0.808052 | 3.743074e-50 | 2.118510e-47 |
| MsG0280008715.01 | MsG0380016527.01 | 0.820256 | 7.550961e-53 | 5.944654e-50 |
| MsG0280008715.01 | MsG0380017253.01 | -0.811879 | 5.613927e-51 | 3.515748e-48 |
| MsG0280008715.01 | MsG0480018232.01 | 0.817732 | 2.830758e-52 | 2.078264e-49 |
| MsG0280008715.01 | MsG0480020689.01 | 0.845266 | 4.521911e-59 | 7.494470e-56 |
| MsG0280008715.01 | MsG0480021564.01 | 0.818570 | 1.829219e-52 | 1.374203e-49 |
| MsG0280008715.01 | MsG0480022617.01 | 0.804734 | 1.873814e-49 | 9.736375e-47 |
| MsG0280008715.01 | MsG0480022918.01 | 0.831847 | 1.323305e-55 | 1.453727e-52 |
| MsG0280008715.01 | MsG0580024435.01 | 0.856631 | 2.822905e-62 | 6.780153e-59 |
| MsG0280008715.01 | MsG0580024538.01 | 0.829641 | 4.594503e-55 | 4.730422e-52 |
| MsG0280008715.01 | MsG0580027331.01 | 0.838808 | 2.307249e-57 | 3.125386e-54 |
| MsG0280008715.01 | MsG0580029276.01 | 0.804487 | 2.109836e-49 | 1.089186e-46 |
| MsG0280008715.01 | MsG0680031406.01 | 0.833713 | 4.553992e-56 | 5.287424e-53 |
| MsG0280008715.01 | MsG0680034402.01 | 0.858057 | 1.069374e-62 | 2.694590e-59 |
| MsG0280008715.01 | MsG0680035707.01 | 0.810954 | 8.912969e-51 | 5.447730e-48 |
| MsG0280008715.01 | MsG0780039256.01 | 0.829500 | 4.972913e-55 | 5.098561e-52 |
| MsG0280008715.01 | MsG0780041191.01 | 0.871352 | 7.298992e-67 | 2.935207e-63 |
| MsG0280008715.01 | MsG0780041218.01 | 0.853368 | 2.503025e-61 | 5.395819e-58 |
| MsG0280008715.01 | MsG0780041256.01 | 0.803386 | 3.574216e-49 | 1.793670e-46 |
| MsG0280008715.01 | MsG0780041301.01 | 0.859286 | 4.595700e-63 | 1.207340e-59 |
| MsG0280008715.01 | MsG0780041668.01 | 0.824615 | 7.333856e-54 | 6.530125e-51 |
| MsG0280008715.01 | MsG0880041965.01 | 0.824332 | 8.548728e-54 | 7.548738e-51 |
| MsG0280008715.01 | MsG0880042000.01 | 0.802298 | 5.996032e-49 | 2.926799e-46 |
| MsG0280008715.01 | MsG0880046062.01 | 0.805680 | 1.187588e-49 | 6.322404e-47 |
| MsG0280008715.01 | MsG0880046334.01 | -0.805015 | 1.636736e-49 | 8.565614e-47 |
| MsG0280008715.01 | MsG0880046735.01 | 0.816209 | 6.223405e-52 | 4.382390e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 16 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATATTCCTCTGTATCTTAA+TGG | 0.207748 | 2:+37148061 | MsG0280008715.01.T01:CDS |
| CTATCATTGACTGATGCTAT+TGG | 0.289689 | 2:-37147980 | None:intergenic |
| CATGGAACTGTTCCCATTCC+TGG | 0.335209 | 2:+37148106 | MsG0280008715.01.T01:CDS |
| GAATAGCATCAACATCAAAT+TGG | 0.360652 | 2:-37148033 | None:intergenic |
| CCATTCCTGGTGAGAAGAAT+AGG | 0.401944 | 2:+37148119 | MsG0280008715.01.T01:CDS |
| AACGGTTGGCTAGCACTAAT+CGG | 0.428488 | 2:-37148157 | None:intergenic |
| CCTATTCTTCTCACCAGGAA+TGG | 0.445918 | 2:-37148119 | None:intergenic |
| CTATTCTTCTCACCAGGAAT+GGG | 0.473967 | 2:-37148118 | None:intergenic |
| ATTTCAGAGTTTACAACCCA+TGG | 0.480742 | 2:+37148088 | MsG0280008715.01.T01:CDS |
| CAGGAATGGGAACAGTTCCA+TGG | 0.531089 | 2:-37148105 | None:intergenic |
| TTAAGATACAGAGGAATATG+TGG | 0.557495 | 2:-37148058 | None:intergenic |
| AGGAATGGGAACAGTTCCAT+GGG | 0.596384 | 2:-37148104 | None:intergenic |
| TACATCCTATTCTTCTCACC+AGG | 0.604815 | 2:-37148124 | None:intergenic |
| AGTCGACAATGGTACACAAG+AGG | 0.654966 | 2:-37148193 | None:intergenic |
| AGATACAGAGGAATATGTGG+AGG | 0.659965 | 2:-37148055 | None:intergenic |
| ATATATCCATTAAGATACAG+AGG | 0.661567 | 2:-37148067 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATATATCCATTAAGATACAG+AGG | - | Chr2:37148070-37148089 | None:intergenic | 25.0% |
| CATATTCCTCTGTATCTTAA+TGG | + | Chr2:37148061-37148080 | MsG0280008715.01.T01:CDS | 30.0% | |
| GAATAGCATCAACATCAAAT+TGG | - | Chr2:37148036-37148055 | None:intergenic | 30.0% | |
| TTAAGATACAGAGGAATATG+TGG | - | Chr2:37148061-37148080 | None:intergenic | 30.0% | |
| AAAAAGAGCAAAGTCGACAA+TGG | - | Chr2:37148207-37148226 | None:intergenic | 35.0% | |
| ATTTCAGAGTTTACAACCCA+TGG | + | Chr2:37148088-37148107 | MsG0280008715.01.T01:CDS | 35.0% | |
| ! | CTATCATTGACTGATGCTAT+TGG | - | Chr2:37147983-37148002 | None:intergenic | 35.0% |
| AGAGGATGTCCAAGCAAAAA+CGG | - | Chr2:37148178-37148197 | None:intergenic | 40.0% | |
| AGATACAGAGGAATATGTGG+AGG | - | Chr2:37148058-37148077 | None:intergenic | 40.0% | |
| CTATTCTTCTCACCAGGAAT+GGG | - | Chr2:37148121-37148140 | None:intergenic | 40.0% | |
| TACATCCTATTCTTCTCACC+AGG | - | Chr2:37148127-37148146 | None:intergenic | 40.0% | |
| AGGAATGGGAACAGTTCCAT+GGG | - | Chr2:37148107-37148126 | None:intergenic | 45.0% | |
| AGTCGACAATGGTACACAAG+AGG | - | Chr2:37148196-37148215 | None:intergenic | 45.0% | |
| CCATTCCTGGTGAGAAGAAT+AGG | + | Chr2:37148119-37148138 | MsG0280008715.01.T01:CDS | 45.0% | |
| CCTATTCTTCTCACCAGGAA+TGG | - | Chr2:37148122-37148141 | None:intergenic | 45.0% | |
| GATGTCCAAGCAAAAACGGT+TGG | - | Chr2:37148174-37148193 | None:intergenic | 45.0% | |
| ! | AACGGTTGGCTAGCACTAAT+CGG | - | Chr2:37148160-37148179 | None:intergenic | 45.0% |
| CAGGAATGGGAACAGTTCCA+TGG | - | Chr2:37148108-37148127 | None:intergenic | 50.0% | |
| CATGGAACTGTTCCCATTCC+TGG | + | Chr2:37148106-37148125 | MsG0280008715.01.T01:CDS | 50.0% | |
| !! | GCTAGCCAACCGTTTTTGCT+TGG | + | Chr2:37148166-37148185 | MsG0280008715.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 37147932 | 37148228 | 37147932 | ID=MsG0280008715.01;Name=MsG0280008715.01 |
| Chr2 | mRNA | 37147932 | 37148228 | 37147932 | ID=MsG0280008715.01.T01;Parent=MsG0280008715.01;Name=MsG0280008715.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|98 |
| Chr2 | exon | 37147932 | 37148228 | 37147932 | ID=MsG0280008715.01.T01:exon:10117;Parent=MsG0280008715.01.T01 |
| Chr2 | CDS | 37147932 | 37148228 | 37147932 | ID=MsG0280008715.01.T01:cds;Parent=MsG0280008715.01.T01 |
| Gene Sequence |
| Protein sequence |
