AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009156.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009156.01.T01 MTR_2g054380 97.059 272 5 2 1 271 1 270 0.0 531
MsG0280009156.01.T01 MTR_2g055990 55.263 266 106 4 9 271 4 259 5.83e-91 270
MsG0280009156.01.T01 MTR_4g112370 52.015 273 101 7 5 271 12 260 2.11e-89 266
MsG0280009156.01.T01 MTR_1g053130 55.556 180 69 3 101 271 107 284 1.09e-56 183
MsG0280009156.01.T01 MTR_3g094830 44.186 172 93 2 101 271 90 259 2.52e-52 171
MsG0280009156.01.T01 MTR_8g040690 48.795 166 81 2 107 271 85 247 2.41e-51 168
MsG0280009156.01.T01 MTR_8g040680 39.912 228 104 4 70 271 37 257 1.90e-49 164
MsG0280009156.01.T01 MTR_3g101300 50.000 166 75 3 111 270 72 235 2.33e-49 163
MsG0280009156.01.T01 MTR_7g064810 45.062 162 84 3 110 270 65 222 2.01e-40 140
MsG0280009156.01.T01 MTR_3g086740 34.667 225 132 6 55 270 24 242 2.56e-35 127
MsG0280009156.01.T01 MTR_8g089865 37.423 163 97 4 112 270 59 220 4.91e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009156.01.T01 AT3G25640 48.805 293 99 6 1 271 30 293 9.35e-81 245
MsG0280009156.01.T01 AT3G25640 48.805 293 99 6 1 271 4 267 1.19e-80 244
MsG0280009156.01.T01 AT5G23100 48.047 256 103 5 46 271 22 277 3.84e-70 218
MsG0280009156.01.T01 AT2G41660 52.542 177 73 4 104 271 123 297 1.16e-59 191
MsG0280009156.01.T01 AT2G21990 48.235 170 80 3 105 271 88 252 1.64e-54 177
MsG0280009156.01.T01 AT5G06990 48.588 177 82 2 103 271 86 261 1.08e-51 170
MsG0280009156.01.T01 AT4G39610 46.512 172 82 2 108 271 95 264 6.36e-51 168
MsG0280009156.01.T01 AT5G42680 54.938 162 68 4 111 270 78 236 3.19e-49 163
MsG0280009156.01.T01 AT5G42680 54.938 162 68 4 111 270 78 236 3.19e-49 163
MsG0280009156.01.T01 AT2G37880 47.929 169 83 3 106 270 77 244 3.54e-46 155
MsG0280009156.01.T01 AT1G21050 45.399 163 78 4 112 270 89 244 1.16e-40 141
MsG0280009156.01.T01 AT1G21050 45.399 163 78 4 112 270 125 280 1.20e-40 142
MsG0280009156.01.T01 AT5G65340 42.775 173 81 5 110 270 83 249 9.57e-38 134
MsG0280009156.01.T01 AT1G76610 43.713 167 79 4 106 270 72 225 3.07e-37 131
MsG0280009156.01.T01 AT2G22460 36.646 161 97 3 110 269 85 241 2.71e-31 116

Find 62 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAAGTATGAGAGGGTTAT+TGG 0.220635 2:-46529759 MsG0280009156.01.T01:CDS
TTGGTTAGGGAAATGGCTTC+TGG 0.285014 2:-46530029 MsG0280009156.01.T01:CDS
AGTTGCTTGAAGAACCAATT+TGG 0.286219 2:-46529953 MsG0280009156.01.T01:CDS
AGTCATCTTGGAAAGCAAAA+TGG 0.301808 2:+46530102 None:intergenic
CAGCAATTTCTAATGGCATT+TGG 0.314688 2:+46530258 None:intergenic
TTACAATAAGACCTCCAAAT+TGG 0.326088 2:+46529939 None:intergenic
CTTCTTTCTCCTTCTCTAGA+AGG 0.332135 2:-46530442 MsG0280009156.01.T01:CDS
ACACCAGCACCCATTGAAAT+AGG 0.337206 2:+46529843 None:intergenic
TCCCTAACCAAAACACTTGT+TGG 0.338137 2:+46530041 None:intergenic
AGCAACACCAACAAGTGTTT+TGG 0.352527 2:-46530048 MsG0280009156.01.T01:CDS
TCAAGAGCAGCAATTTCTAA+TGG 0.388276 2:+46530251 None:intergenic
CTAAGTATGAGAGGGTTATT+GGG 0.402385 2:-46529758 MsG0280009156.01.T01:CDS
GGCTCAACTTTGAGTCATCT+TGG 0.402943 2:+46530090 None:intergenic
GAAAATGTGGTTATGCATAT+AGG 0.413946 2:-46529911 MsG0280009156.01.T01:CDS
AGCAAGTAAAGGCAGAGTTC+AGG 0.432013 2:+46529684 None:intergenic
GTCCACGACGGTATCCGAAA+AGG 0.435618 2:+46530129 None:intergenic
GCTAGCTCAATTAAGAATGT+AGG 0.445992 2:+46530068 None:intergenic
TCTCTAGAAGGTATTTCCAT+TGG 0.449753 2:-46530430 MsG0280009156.01.T01:CDS
TCAGGACCACCACTACCAAC+AGG 0.456710 2:+46529702 None:intergenic
ATTTGGAGGTCTTATTGTAA+TGG 0.458861 2:-46529936 MsG0280009156.01.T01:CDS
TGGAGTGTGAGAAGAAAGGA+GGG 0.460730 2:-46529989 MsG0280009156.01.T01:CDS
TCACCTCCATTCCCACTACT+TGG 0.485738 2:+46529810 None:intergenic
TTGGAGTGTGAGAAGAAAGG+AGG 0.490295 2:-46529990 MsG0280009156.01.T01:CDS
GGCTTTGGATGAAGATGATG+AGG 0.491809 2:-46530399 MsG0280009156.01.T01:CDS
TATTGTAATGGAAGAAAATG+TGG 0.509766 2:-46529924 MsG0280009156.01.T01:CDS
CTAGCTCAATTAAGAATGTA+GGG 0.510058 2:+46530069 None:intergenic
TGCTTGAAGAACCAATTTGG+AGG 0.514952 2:-46529950 MsG0280009156.01.T01:CDS
GTTAAACTCTAAGCAAGTAA+AGG 0.515924 2:+46529673 None:intergenic
AGCTGTGGAACCTATTTCAA+TGG 0.524917 2:-46529853 MsG0280009156.01.T01:CDS
TCCACGACGGTATCCGAAAA+GGG 0.528553 2:+46530130 None:intergenic
CGTTCACTTCACAGTTCTAG+TGG 0.530900 2:-46530170 MsG0280009156.01.T01:CDS
CCATTCCCACTACTTGGCAT+TGG 0.536474 2:+46529816 None:intergenic
GAACCTATTTCAATGGGTGC+TGG 0.538761 2:-46529846 MsG0280009156.01.T01:CDS
GCTGTGGAACCTATTTCAAT+GGG 0.541783 2:-46529852 MsG0280009156.01.T01:CDS
TGGAGTGGTGAGGATTGCAT+TGG 0.543210 2:-46530009 MsG0280009156.01.T01:CDS
GGGAATGGAGGTGAGTTTGA+AGG 0.549280 2:-46529801 MsG0280009156.01.T01:CDS
GCATTGGAGTGTGAGAAGAA+AGG 0.552731 2:-46529993 MsG0280009156.01.T01:CDS
TTCTAGTGGGACACGTATGG+TGG 0.556842 2:-46530156 MsG0280009156.01.T01:CDS
CCAATGCCAAGTAGTGGGAA+TGG 0.559271 2:-46529816 MsG0280009156.01.T01:CDS
ATGAACCCTGTTGGTAGTGG+TGG 0.559559 2:-46529708 MsG0280009156.01.T01:CDS
AGAAGAGTCATGTGGTGACT+TGG 0.563167 2:+46530460 None:intergenic
TATGAGAGCTAAGTATGAGA+GGG 0.565675 2:-46529766 MsG0280009156.01.T01:CDS
ATGGAAATACCTTCTAGAGA+AGG 0.577098 2:+46530433 None:intergenic
CAGTTCTAGTGGGACACGTA+TGG 0.583654 2:-46530159 MsG0280009156.01.T01:CDS
ATATGAGAGCTAAGTATGAG+AGG 0.585257 2:-46529767 MsG0280009156.01.T01:CDS
ATGATGAACCCTGTTGGTAG+TGG 0.586626 2:-46529711 MsG0280009156.01.T01:CDS
TTCTACATGATGAACCCTGT+TGG 0.588457 2:-46529717 MsG0280009156.01.T01:CDS
ATGGAAGATATTGAAAGCTG+TGG 0.589180 2:-46529868 MsG0280009156.01.T01:CDS
TAGGGAAATGGCTTCTGGAG+TGG 0.612183 2:-46530024 MsG0280009156.01.T01:CDS
ATGAATGTGGATCAGAGGAA+TGG 0.616115 2:-46529887 MsG0280009156.01.T01:CDS
TAGGCATGAATGTGGATCAG+AGG 0.616854 2:-46529892 MsG0280009156.01.T01:CDS
AAGGAGAAAGAAGAGTCATG+TGG 0.646233 2:+46530452 None:intergenic
GTTCACTTCACAGTTCTAGT+GGG 0.646627 2:-46530169 MsG0280009156.01.T01:CDS
TCTAGTGGGACACGTATGGT+GGG 0.646952 2:-46530155 MsG0280009156.01.T01:CDS
ATGCCAAGTAGTGGGAATGG+AGG 0.656575 2:-46529813 MsG0280009156.01.T01:CDS
TGTTGCCAATGCCAAGTAGT+GGG 0.657243 2:-46529821 MsG0280009156.01.T01:CDS
CAGGACCACCACTACCAACA+GGG 0.658555 2:+46529703 None:intergenic
AAATGGCTTCTGGAGTGGTG+AGG 0.662532 2:-46530019 MsG0280009156.01.T01:CDS
GTGTTGCCAATGCCAAGTAG+TGG 0.663696 2:-46529822 MsG0280009156.01.T01:CDS
TATGCATATAGGCATGAATG+TGG 0.680421 2:-46529900 MsG0280009156.01.T01:CDS
TGTTGGTGGTGAGAAAGAAG+AGG 0.693894 2:-46530318 MsG0280009156.01.T01:CDS
CAAAATGGACATGTCCACGA+CGG 0.712176 2:+46530117 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTAATGGAAGAAAATG+TGG - Chr2:46530217-46530236 MsG0280009156.01.T01:CDS 25.0%
CTAGCTCAATTAAGAATGTA+GGG + Chr2:46530075-46530094 None:intergenic 30.0%
GAAAATGTGGTTATGCATAT+AGG - Chr2:46530230-46530249 MsG0280009156.01.T01:CDS 30.0%
GTATTTCCATTGGAAAAAGA+AGG - Chr2:46529721-46529740 MsG0280009156.01.T01:CDS 30.0%
TTACAATAAGACCTCCAAAT+TGG + Chr2:46530205-46530224 None:intergenic 30.0%
!! ATTTGGAGGTCTTATTGTAA+TGG - Chr2:46530205-46530224 MsG0280009156.01.T01:CDS 30.0%
AGTCATCTTGGAAAGCAAAA+TGG + Chr2:46530042-46530061 None:intergenic 35.0%
AGTTGCTTGAAGAACCAATT+TGG - Chr2:46530188-46530207 MsG0280009156.01.T01:CDS 35.0%
ATATGAGAGCTAAGTATGAG+AGG - Chr2:46530374-46530393 MsG0280009156.01.T01:CDS 35.0%
ATGGAAGATATTGAAAGCTG+TGG - Chr2:46530273-46530292 MsG0280009156.01.T01:CDS 35.0%
CAGCAATTTCTAATGGCATT+TGG + Chr2:46529886-46529905 None:intergenic 35.0%
CTAAGTATGAGAGGGTTATT+GGG - Chr2:46530383-46530402 MsG0280009156.01.T01:CDS 35.0%
GCTAGCTCAATTAAGAATGT+AGG + Chr2:46530076-46530095 None:intergenic 35.0%
TATGAGAGCTAAGTATGAGA+GGG - Chr2:46530375-46530394 MsG0280009156.01.T01:CDS 35.0%
TATGCATATAGGCATGAATG+TGG - Chr2:46530241-46530260 MsG0280009156.01.T01:CDS 35.0%
TCAAGAGCAGCAATTTCTAA+TGG + Chr2:46529893-46529912 None:intergenic 35.0%
! ATGGAAATACCTTCTAGAGA+AGG + Chr2:46529711-46529730 None:intergenic 35.0%
! GATGATGATGATGATGATGT+TGG - Chr2:46529806-46529825 MsG0280009156.01.T01:CDS 35.0%
! TTCATCATCACATTTTCCTG+AGG - Chr2:46529784-46529803 MsG0280009156.01.T01:CDS 35.0%
!! TCTCTAGAAGGTATTTCCAT+TGG - Chr2:46529711-46529730 MsG0280009156.01.T01:CDS 35.0%
!!! AAGTGTTTTGGTTAGGGAAA+TGG - Chr2:46530105-46530124 MsG0280009156.01.T01:CDS 35.0%
!!! ACCAACAAGTGTTTTGGTTA+GGG - Chr2:46530099-46530118 MsG0280009156.01.T01:CDS 35.0%
AAGGAGAAAGAAGAGTCATG+TGG + Chr2:46529692-46529711 None:intergenic 40.0%
AGCTGTGGAACCTATTTCAA+TGG - Chr2:46530288-46530307 MsG0280009156.01.T01:CDS 40.0%
ATGAATGTGGATCAGAGGAA+TGG - Chr2:46530254-46530273 MsG0280009156.01.T01:CDS 40.0%
CCATTGGAAAAAGAAGGCTT+TGG - Chr2:46529727-46529746 MsG0280009156.01.T01:CDS 40.0%
CTTCTTTCTCCTTCTCTAGA+AGG - Chr2:46529699-46529718 MsG0280009156.01.T01:CDS 40.0%
GCTAAGTATGAGAGGGTTAT+TGG - Chr2:46530382-46530401 MsG0280009156.01.T01:CDS 40.0%
GCTGTGGAACCTATTTCAAT+GGG - Chr2:46530289-46530308 MsG0280009156.01.T01:CDS 40.0%
TCATCATCATCATCATCCTC+AGG + Chr2:46529803-46529822 None:intergenic 40.0%
TCCCTAACCAAAACACTTGT+TGG + Chr2:46530103-46530122 None:intergenic 40.0%
TGCTTGAAGAACCAATTTGG+AGG - Chr2:46530191-46530210 MsG0280009156.01.T01:CDS 40.0%
TTCTACATGATGAACCCTGT+TGG - Chr2:46530424-46530443 MsG0280009156.01.T01:CDS 40.0%
! AGCAACACCAACAAGTGTTT+TGG - Chr2:46530093-46530112 MsG0280009156.01.T01:CDS 40.0%
! CCAAAGCCTTCTTTTTCCAA+TGG + Chr2:46529730-46529749 None:intergenic 40.0%
! GATGATGATGATGATGTTGG+TGG - Chr2:46529809-46529828 MsG0280009156.01.T01:CDS 40.0%
! GTTCACTTCACAGTTCTAGT+GGG - Chr2:46529972-46529991 MsG0280009156.01.T01:CDS 40.0%
!!! CACCAACAAGTGTTTTGGTT+AGG - Chr2:46530098-46530117 MsG0280009156.01.T01:CDS 40.0%
ACACCAGCACCCATTGAAAT+AGG + Chr2:46530301-46530320 None:intergenic 45.0%
AGAAGAGTCATGTGGTGACT+TGG + Chr2:46529684-46529703 None:intergenic 45.0%
AGCAAGTAAAGGCAGAGTTC+AGG + Chr2:46530460-46530479 None:intergenic 45.0%
CAAAATGGACATGTCCACGA+CGG + Chr2:46530027-46530046 None:intergenic 45.0%
GAACCTATTTCAATGGGTGC+TGG - Chr2:46530295-46530314 MsG0280009156.01.T01:CDS 45.0%
GCATTGGAGTGTGAGAAGAA+AGG - Chr2:46530148-46530167 MsG0280009156.01.T01:CDS 45.0%
GGCTCAACTTTGAGTCATCT+TGG + Chr2:46530054-46530073 None:intergenic 45.0%
TAGGCATGAATGTGGATCAG+AGG - Chr2:46530249-46530268 MsG0280009156.01.T01:CDS 45.0%
TGGAGTGTGAGAAGAAAGGA+GGG - Chr2:46530152-46530171 MsG0280009156.01.T01:CDS 45.0%
TGTTGCCAATGCCAAGTAGT+GGG - Chr2:46530320-46530339 MsG0280009156.01.T01:CDS 45.0%
TTGGAGTGTGAGAAGAAAGG+AGG - Chr2:46530151-46530170 MsG0280009156.01.T01:CDS 45.0%
TTGGTTAGGGAAATGGCTTC+TGG - Chr2:46530112-46530131 MsG0280009156.01.T01:CDS 45.0%
! ATGATGAACCCTGTTGGTAG+TGG - Chr2:46530430-46530449 MsG0280009156.01.T01:CDS 45.0%
! CGTTCACTTCACAGTTCTAG+TGG - Chr2:46529971-46529990 MsG0280009156.01.T01:CDS 45.0%
!! GGCTTTGGATGAAGATGATG+AGG - Chr2:46529742-46529761 MsG0280009156.01.T01:CDS 45.0%
!! TGTTGGTGGTGAGAAAGAAG+AGG - Chr2:46529823-46529842 MsG0280009156.01.T01:CDS 45.0%
CCATTCCCACTACTTGGCAT+TGG + Chr2:46530328-46530347 None:intergenic 50.0%
GGGAATGGAGGTGAGTTTGA+AGG - Chr2:46530340-46530359 MsG0280009156.01.T01:CDS 50.0%
GTGTTGCCAATGCCAAGTAG+TGG - Chr2:46530319-46530338 MsG0280009156.01.T01:CDS 50.0%
TCACCTCCATTCCCACTACT+TGG + Chr2:46530334-46530353 None:intergenic 50.0%
TCCACGACGGTATCCGAAAA+GGG + Chr2:46530014-46530033 None:intergenic 50.0%
TCTAGTGGGACACGTATGGT+GGG - Chr2:46529986-46530005 MsG0280009156.01.T01:CDS 50.0%
TGGAGTGGTGAGGATTGCAT+TGG - Chr2:46530132-46530151 MsG0280009156.01.T01:CDS 50.0%
TTCTAGTGGGACACGTATGG+TGG - Chr2:46529985-46530004 MsG0280009156.01.T01:CDS 50.0%
! ATGCCAAGTAGTGGGAATGG+AGG - Chr2:46530328-46530347 MsG0280009156.01.T01:CDS 50.0%
! CAGTTCTAGTGGGACACGTA+TGG - Chr2:46529982-46530001 MsG0280009156.01.T01:CDS 50.0%
! CCAATGCCAAGTAGTGGGAA+TGG - Chr2:46530325-46530344 MsG0280009156.01.T01:CDS 50.0%
! TAGGGAAATGGCTTCTGGAG+TGG - Chr2:46530117-46530136 MsG0280009156.01.T01:CDS 50.0%
!! AAATGGCTTCTGGAGTGGTG+AGG - Chr2:46530122-46530141 MsG0280009156.01.T01:CDS 50.0%
!! ATGAACCCTGTTGGTAGTGG+TGG - Chr2:46530433-46530452 MsG0280009156.01.T01:CDS 50.0%
CAGGACCACCACTACCAACA+GGG + Chr2:46530441-46530460 None:intergenic 55.0%
GTCCACGACGGTATCCGAAA+AGG + Chr2:46530015-46530034 None:intergenic 55.0%
TCAGGACCACCACTACCAAC+AGG + Chr2:46530442-46530461 None:intergenic 55.0%
! ACCCTTTTCGGATACCGTCG+TGG - Chr2:46530010-46530029 MsG0280009156.01.T01:CDS 55.0%
!!! CGTATGGTGGGCACCCTTTT+CGG - Chr2:46529998-46530017 MsG0280009156.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 46529674 46530489 46529674 ID=MsG0280009156.01;Name=MsG0280009156.01
Chr2 mRNA 46529674 46530489 46529674 ID=MsG0280009156.01.T01;Parent=MsG0280009156.01;Name=MsG0280009156.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|271
Chr2 exon 46529674 46530489 46529674 ID=MsG0280009156.01.T01:exon:15827;Parent=MsG0280009156.01.T01
Chr2 CDS 46529674 46530489 46529674 ID=MsG0280009156.01.T01:cds;Parent=MsG0280009156.01.T01
Gene Sequence

>MsG0280009156.01.T01

ATGGCAGCCAAGTCACCACATGACTCTTCTTTCTCCTTCTCTAGAAGGTATTTCCATTGGAAAAAGAAGGCTTTGGATGAAGATGATGAGGAAGAAATACTCAATATAAGTTCATCATCACATTTTCCTGAGGATGATGATGATGATGATGTTGGTGGTGAGAAAGAAGAGGTTCATCATCATCGACATCATCATAATCATTTAAGAATCCAAATGCCATTAGAAATTGCTGCTCTTGAAAAGAAGAAGAATTCAAAATCAAAGTTCAAGTCAGCACTAACAATTCTCACAAAGAATCGTTCACTTCACAGTTCTAGTGGGACACGTATGGTGGGCACCCTTTTCGGATACCGTCGTGGACATGTCCATTTTGCTTTCCAAGATGACTCAAAGTTGAGCCCTACATTCTTAATTGAGCTAGCAACACCAACAAGTGTTTTGGTTAGGGAAATGGCTTCTGGAGTGGTGAGGATTGCATTGGAGTGTGAGAAGAAAGGAGGGAGAAAGAGTTTGAAGTTGCTTGAAGAACCAATTTGGAGGTCTTATTGTAATGGAAGAAAATGTGGTTATGCATATAGGCATGAATGTGGATCAGAGGAATGGAAGATATTGAAAGCTGTGGAACCTATTTCAATGGGTGCTGGTGTGTTGCCAATGCCAAGTAGTGGGAATGGAGGTGAGTTTGAAGGTGAGCTCATGTATATGAGAGCTAAGTATGAGAGGGTTATTGGGTCTAAAGATTCTGAAGCTTTCTACATGATGAACCCTGTTGGTAGTGGTGGTCCTGAACTCTGCCTTTACTTGCTTAGAGTTTAA

Protein sequence

>MsG0280009156.01.T01

MAAKSPHDSSFSFSRRYFHWKKKALDEDDEEEILNISSSSHFPEDDDDDDVGGEKEEVHHHRHHHNHLRIQMPLEIAALEKKKNSKSKFKSALTILTKNRSLHSSSGTRMVGTLFGYRRGHVHFAFQDDSKLSPTFLIELATPTSVLVREMASGVVRIALECEKKGGRKSLKLLEEPIWRSYCNGRKCGYAYRHECGSEEWKILKAVEPISMGAGVLPMPSSGNGGEFEGELMYMRAKYERVIGSKDSEAFYMMNPVGSGGPELCLYLLRV*