AlfalfaGEDB | MsG0680030763.01

M. sativa cultivar ZhongmuNo.1 / MsG0680030763.01

KEGG: K18980
GO: GO:0008270 GO:0016491 GO:0055114
PFAM: PF08240 PF13602

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030763.01.T01	XP_003618487.1	95.625	320	14	0	1	320	1	320	0	585
MsG0680030763.01.T02	XP_003618487.1	95.625	320	14	0	1	320	1	320	0	585

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030763.01.T01	Q84V25	73.354	319	85	0	2	320	4	322	3.62E-161	455
MsG0680030763.01.T02	Q84V25	73.354	319	85	0	2	320	4	322	3.62E-161	455

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030763.01.T01	G7KKH6	95.625	320	14	0	1	320	1	320	0.0	585
MsG0680030763.01.T02	G7KKH6	95.625	320	14	0	1	320	1	320	0.0	585

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048700.01	MsG0680030763.01	0.822601	2.170607e-53	1.825468e-50
MsG0080048701.01	MsG0680030763.01	0.801547	8.550693e-49	4.094729e-46
MsG0080049140.01	MsG0680030763.01	0.826800	2.224097e-54	2.107423e-51
MsG0180000353.01	MsG0680030763.01	0.811235	7.749911e-51	4.771555e-48
MsG0180000360.01	MsG0680030763.01	0.806616	7.545245e-50	4.113873e-47
MsG0180000373.01	MsG0680030763.01	0.828382	9.274928e-55	9.204220e-52
MsG0180002421.01	MsG0680030763.01	0.808220	3.446958e-50	1.959683e-47
MsG0180003427.01	MsG0680030763.01	0.850179	2.006857e-60	3.897903e-57
MsG0180003796.01	MsG0680030763.01	0.803411	3.531565e-49	1.773438e-46
MsG0180003858.01	MsG0680030763.01	0.801985	6.954921e-49	3.367595e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030763.01.T01	MTR_6g012170	95.625	320	14	0	1	320	1	320	0.0	585
MsG0680030763.01.T01	MTR_6g012160	92.857	294	21	0	1	294	1	294	0.0	522
MsG0680030763.01.T01	MTR_8g064610	68.471	314	98	1	7	320	72	384	3.97e-154	437
MsG0680030763.01.T01	MTR_7g034395	72.492	309	85	0	12	320	9	317	1.31e-146	415
MsG0680030763.01.T01	MTR_8g035630	46.624	311	160	5	12	320	5	311	8.39e-88	267
MsG0680030763.01.T01	MTR_8g035880	47.231	307	156	5	12	316	5	307	3.31e-87	265
MsG0680030763.01.T01	MTR_6g012140	87.234	141	18	0	179	319	3	143	2.57e-84	251
MsG0680030763.01.T01	MTR_1319s0010	71.963	107	30	0	73	179	3	109	1.13e-41	140
MsG0680030763.01.T01	MTR_7g055743	34.132	334	169	11	13	317	57	368	4.31e-38	139
MsG0680030763.01.T01	MTR_4g036310	35.866	329	173	12	16	317	11	328	2.18e-37	136

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0680030763.01.T01	AT1G23740	65.079	315	109	1	6	320	73	386	4.23e-143	409
MsG0680030763.01.T01	AT4G13010	32.530	332	182	10	16	318	11	329	6.28e-35	130
MsG0680030763.01.T01	AT3G15090	32.685	257	159	8	1	251	22	270	4.02e-30	117
MsG0680030763.01.T01	AT4G21580	28.319	339	194	12	12	317	2	324	2.94e-25	103
MsG0680030763.01.T01	AT4G21580	27.811	338	191	12	12	317	2	318	3.66e-24	100
MsG0680030763.01.T01	AT3G56460	29.154	319	178	11	33	316	31	336	2.36e-21	93.2
MsG0680030763.01.T01	AT1G49670	26.316	323	183	12	35	320	316	620	1.17e-18	87.0
MsG0680030763.01.T01	AT1G49670	25.291	344	183	12	35	320	316	643	6.32e-18	84.7
MsG0680030763.01.T01	AT3G45770	28.458	253	157	8	11	250	45	286	4.51e-16	78.6
MsG0680030763.01.T01	AT5G63620	26.224	286	144	10	29	251	63	344	3.15e-11	64.3

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Find 21 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACTTGATCTTCTCTAGGGTG+TGG	0.546580	6:+8731324	None:intergenic
AGAAGATCAAGTGCTCATCA+AGG	0.612803	6:-8731313	MsG0680030763.01.T01:CDS
AGAGCCCTCTTAATATCAAC+AGG	0.464124	6:+8731270	None:intergenic
ATGAGCACTTGATCTTCTCT+AGG	0.411783	6:+8731318	None:intergenic
ATGATCACTTTGCTACTAAG+AGG	0.493924	6:+8731447	None:intergenic
CTTAATATCAACAGGATTAA+GGG	0.365726	6:+8731278	None:intergenic
CTTCTCTAGGGTGTGGTGTA+GGG	0.431980	6:+8731331	None:intergenic
GAGGGCTCTTGGTCATTTCA+AGG	0.427218	6:-8731256	MsG0680030763.01.T01:CDS
GATTGCAATCTTACTGGCAA+AGG	0.597841	6:+8731222	None:intergenic
GCTTGGGTTTACTCTCAATA+TGG	0.324385	6:-8731390	MsG0680030763.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAATCTATACTTGTTCTTGC+AGG	-	Chr6:8730325-8730344	MsG0680030763.01.T01:intron	30.0%
AAGTTCTTCATAATTCTCCT+TGG	+	Chr6:8730933-8730952	None:intergenic	30.0%
AATCAAGCACATCAATTTCA+TGG	-	Chr6:8731379-8731398	MsG0680030763.01.T01:CDS	30.0%
ACAGTATGAGAAAATGGAAA+GGG	+	Chr6:8731284-8731303	None:intergenic	30.0%
AGCTCTATTTGTATTGAGAT+AGG	+	Chr6:8731318-8731337	None:intergenic	30.0%
ATCCATTGGTCAATATAAGT+TGG	-	Chr6:8730729-8730748	MsG0680030763.01.T01:CDS	30.0%
ATTATTCATGCTCATGGAAT+GGG	+	Chr6:8731362-8731381	None:intergenic	30.0%
ATTGACCAATGGATAAGTTA+AGG	+	Chr6:8730723-8730742	None:intergenic	30.0%
CTCAATACAAATAGAGCTAT+TGG	-	Chr6:8731320-8731339	MsG0680030763.01.T01:CDS	30.0%
GCAATAATTACTGCTTATCA+AGG	-	Chr6:8730274-8730293	MsG0680030763.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr6	gene	8729347	8731540	8729347	ID=MsG0680030763.01;Name=MsG0680030763.01
Chr6	mRNA	8729347	8731540	8729347	ID=MsG0680030763.01.T01;Parent=MsG0680030763.01;Name=MsG0680030763.01.T01;_AED=0.44;_eAED=0.44;_QI=71\|1\|1\|1\|0.75\|0.6\|5\|170\|320
Chr6	exon	8731236	8731461	8731236	ID=MsG0680030763.01.T01:exon:7240;Parent=MsG0680030763.01.T01
Chr6	exon	8731483	8731540	8731483	ID=MsG0680030763.01.T01:exon:7239;Parent=MsG0680030763.01.T01
Chr6	five_prime_UTR	8731483	8731540	8731483	ID=MsG0680030763.01.T01:five_prime_utr;Parent=MsG0680030763.01.T01
Chr6	five_prime_UTR	8731449	8731461	8731449	ID=MsG0680030763.01.T01:five_prime_utr;Parent=MsG0680030763.01.T01
Chr6	CDS	8731236	8731448	8731236	ID=MsG0680030763.01.T01:cds;Parent=MsG0680030763.01.T01
Chr6	CDS	8730509	8730832	8730509	ID=MsG0680030763.01.T01:cds;Parent=MsG0680030763.01.T01
Chr6	CDS	8729901	8730060	8729901	ID=MsG0680030763.01.T01:cds;Parent=MsG0680030763.01.T01
Chr6	CDS	8729517	8729782	8729517	ID=MsG0680030763.01.T01:cds;Parent=MsG0680030763.01.T01
Chr6	three_prime_UTR	8729347	8729516	8729347	ID=MsG0680030763.01.T01:three_prime_utr;Parent=MsG0680030763.01.T01
Chr6	mRNA	8729347	8731535	8729347	ID=MsG0680030763.01.T02;Parent=MsG0680030763.01;Name=MsG0680030763.01.T02;_AED=0.44;_eAED=0.44;_QI=87\|1\|1\|1\|1\|1\|4\|170\|320
Chr6	exon	8731236	8731535	8731236	ID=MsG0680030763.01.T02:exon:7244;Parent=MsG0680030763.01.T02
Chr6	five_prime_UTR	8731449	8731535	8731449	ID=MsG0680030763.01.T02:five_prime_utr;Parent=MsG0680030763.01.T02
Chr6	CDS	8731236	8731448	8731236	ID=MsG0680030763.01.T02:cds;Parent=MsG0680030763.01.T02
Chr6	CDS	8730509	8730832	8730509	ID=MsG0680030763.01.T02:cds;Parent=MsG0680030763.01.T02
Chr6	CDS	8729901	8730060	8729901	ID=MsG0680030763.01.T02:cds;Parent=MsG0680030763.01.T02
Chr6	CDS	8729517	8729782	8729517	ID=MsG0680030763.01.T02:cds;Parent=MsG0680030763.01.T02
Chr6	three_prime_UTR	8729347	8729516	8729347	ID=MsG0680030763.01.T02:three_prime_utr;Parent=MsG0680030763.01.T02

Gene Sequence

>MsG0680030763.01.T01

ATGGCAAGCACACATAGTATTCCATCTCATACAAAAGCTTGGGTTTACTCTCAATATGGTAACATAGAAGAAATTCTAAAGTTTGATCCTAATGTCCCTACACCACACCCTAGAGAAGATCAAGTGCTCATCAAGGTTGTAGCTGCTGCCCTTAATCCTGTTGATATTAAGAGGGCTCTTGGTCATTTCAAGGACATTGACTCTCCTTTGCCAACTGTGCCAGGATATGATGTAGCTGGTGTGGTGGTTAGTGTTGGAGACCAAGTAAAGAAATTTAAGGTTGGAGATGAAGTTTATGGTGATATCAATGAGATTACTTTACACAATCCAAAAACTATTGGTTCTTTGTCAGAGTATACTGTTGCTGAAGAAAAAGTACTTGCTCACAAACCATCAAATTTGAGTTTTGTTGAAGCTGCTAGTCTCCCTTTAGCAATAATTACTGCTTATCAAGGACTTGAAAGAGTTGAATTTTCTGCTGGTAAATCTATACTTGTTCTTGCAGGTGCTGGAGGAGTTGGATCTCTTGTTATTCAGCTAGCCAAGCATGTTTTTGAGGCATCTAGAATTGCAGCTACTGCTAGCACAACAAAACTTGAATTTTTGAGAAAATTGGGAGCAGACTTGGCAATTGATTATACCAAGGAGAATTATGAAGAACTTACTGAAAAATTTGATGTGGTGTATGATGCAGTAGGTGATAGTGAGAGGGCAGTGAAGGCTGCCAATGAAGGAGGAAAAGTTGTGACAATATTACCACCTGGAACTCCACCAGCTATCCCTTTCTTACTCACTTCAGATGGAGCTGTGTTGGAGAAATTGCTCCCTTATTTGGAAAATGGCAAAGTGAAACCAATATTGGACCCTAAGAGTCCCTTTCCATTTTCTCATACTGTGGAAGCATTTACCTATCTCAATACAAATAGAGCTATTGGGAAAATAGTTATTCATCCCATTCCATGA

>MsG0680030763.01.T02

Protein sequence

>MsG0680030763.01.T01

MASTHSIPSHTKAWVYSQYGNIEEILKFDPNVPTPHPREDQVLIKVVAAALNPVDIKRALGHFKDIDSPLPTVPGYDVAGVVVSVGDQVKKFKVGDEVYGDINEITLHNPKTIGSLSEYTVAEEKVLAHKPSNLSFVEAASLPLAIITAYQGLERVEFSAGKSILVLAGAGGVGSLVIQLAKHVFEASRIAATASTTKLEFLRKLGADLAIDYTKENYEELTEKFDVVYDAVGDSERAVKAANEGGKVVTILPPGTPPAIPFLLTSDGAVLEKLLPYLENGKVKPILDPKSPFPFSHTVEAFTYLNTNRAIGKIVIHPIP*

>MsG0680030763.01.T02