AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280009118.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009118.01.T01 MTR_2g054380 97.059 272 5 2 1 271 1 270 0.0 531
MsG0280009118.01.T01 MTR_2g055990 55.263 266 106 4 9 271 4 259 5.83e-91 270
MsG0280009118.01.T01 MTR_4g112370 52.015 273 101 7 5 271 12 260 2.11e-89 266
MsG0280009118.01.T01 MTR_1g053130 55.556 180 69 3 101 271 107 284 1.09e-56 183
MsG0280009118.01.T01 MTR_3g094830 44.186 172 93 2 101 271 90 259 2.52e-52 171
MsG0280009118.01.T01 MTR_8g040690 48.795 166 81 2 107 271 85 247 2.41e-51 168
MsG0280009118.01.T01 MTR_8g040680 39.912 228 104 4 70 271 37 257 1.90e-49 164
MsG0280009118.01.T01 MTR_3g101300 50.000 166 75 3 111 270 72 235 2.33e-49 163
MsG0280009118.01.T01 MTR_7g064810 45.062 162 84 3 110 270 65 222 2.01e-40 140
MsG0280009118.01.T01 MTR_3g086740 34.667 225 132 6 55 270 24 242 2.56e-35 127
MsG0280009118.01.T01 MTR_8g089865 37.423 163 97 4 112 270 59 220 4.91e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280009118.01.T01 AT3G25640 48.805 293 99 6 1 271 30 293 9.35e-81 245
MsG0280009118.01.T01 AT3G25640 48.805 293 99 6 1 271 4 267 1.19e-80 244
MsG0280009118.01.T01 AT5G23100 48.047 256 103 5 46 271 22 277 3.84e-70 218
MsG0280009118.01.T01 AT2G41660 52.542 177 73 4 104 271 123 297 1.16e-59 191
MsG0280009118.01.T01 AT2G21990 48.235 170 80 3 105 271 88 252 1.64e-54 177
MsG0280009118.01.T01 AT5G06990 48.588 177 82 2 103 271 86 261 1.08e-51 170
MsG0280009118.01.T01 AT4G39610 46.512 172 82 2 108 271 95 264 6.36e-51 168
MsG0280009118.01.T01 AT5G42680 54.938 162 68 4 111 270 78 236 3.19e-49 163
MsG0280009118.01.T01 AT5G42680 54.938 162 68 4 111 270 78 236 3.19e-49 163
MsG0280009118.01.T01 AT2G37880 47.929 169 83 3 106 270 77 244 3.54e-46 155
MsG0280009118.01.T01 AT1G21050 45.399 163 78 4 112 270 89 244 1.16e-40 141
MsG0280009118.01.T01 AT1G21050 45.399 163 78 4 112 270 125 280 1.20e-40 142
MsG0280009118.01.T01 AT5G65340 42.775 173 81 5 110 270 83 249 9.57e-38 134
MsG0280009118.01.T01 AT1G76610 43.713 167 79 4 106 270 72 225 3.07e-37 131
MsG0280009118.01.T01 AT2G22460 36.646 161 97 3 110 269 85 241 2.71e-31 116

Find 62 sgRNAs with CRISPR-Local

Find 73 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTAAGTATGAGAGGGTTAT+TGG 0.220635 2:+45677026 MsG0280009118.01.T01:CDS
TTGGTTAGGGAAATGGCTTC+TGG 0.285014 2:+45676756 MsG0280009118.01.T01:CDS
AGTTGCTTGAAGAACCAATT+TGG 0.286219 2:+45676832 MsG0280009118.01.T01:CDS
AGTCATCTTGGAAAGCAAAA+TGG 0.301808 2:-45676683 None:intergenic
CAGCAATTTCTAATGGCATT+TGG 0.314688 2:-45676527 None:intergenic
TTACAATAAGACCTCCAAAT+TGG 0.326088 2:-45676846 None:intergenic
CTTCTTTCTCCTTCTCTAGA+AGG 0.332135 2:+45676343 MsG0280009118.01.T01:CDS
ACACCAGCACCCATTGAAAT+AGG 0.337206 2:-45676942 None:intergenic
TCCCTAACCAAAACACTTGT+TGG 0.338137 2:-45676744 None:intergenic
AGCAACACCAACAAGTGTTT+TGG 0.352527 2:+45676737 MsG0280009118.01.T01:CDS
TCAAGAGCAGCAATTTCTAA+TGG 0.388276 2:-45676534 None:intergenic
CTAAGTATGAGAGGGTTATT+GGG 0.402385 2:+45677027 MsG0280009118.01.T01:CDS
GGCTCAACTTTGAGTCATCT+TGG 0.402943 2:-45676695 None:intergenic
GAAAATGTGGTTATGCATAT+AGG 0.413946 2:+45676874 MsG0280009118.01.T01:CDS
AGCAAGTAAAGGCAGAGTTC+AGG 0.432013 2:-45677101 None:intergenic
GTCCACGACGGTATCCGAAA+AGG 0.435618 2:-45676656 None:intergenic
GCTAGCTCAATTAAGAATGT+AGG 0.445992 2:-45676717 None:intergenic
TCTCTAGAAGGTATTTCCAT+TGG 0.449753 2:+45676355 MsG0280009118.01.T01:CDS
TCAGGACCACCACTACCAAC+AGG 0.456710 2:-45677083 None:intergenic
ATTTGGAGGTCTTATTGTAA+TGG 0.458861 2:+45676849 MsG0280009118.01.T01:CDS
TGGAGTGTGAGAAGAAAGGA+GGG 0.460730 2:+45676796 MsG0280009118.01.T01:CDS
TCACCTCCATTCCCACTACT+TGG 0.485738 2:-45676975 None:intergenic
TTGGAGTGTGAGAAGAAAGG+AGG 0.490295 2:+45676795 MsG0280009118.01.T01:CDS
TTTCAATATCTTCCATTCCT+CGG 0.491311 2:-45676910 None:intergenic
GGCTTTGGATGAAGATGATG+AGG 0.491809 2:+45676386 MsG0280009118.01.T01:CDS
TATTGTAATGGAAGAAAATG+TGG 0.509766 2:+45676861 MsG0280009118.01.T01:CDS
CTAGCTCAATTAAGAATGTA+GGG 0.510058 2:-45676716 None:intergenic
TGCTTGAAGAACCAATTTGG+AGG 0.514952 2:+45676835 MsG0280009118.01.T01:CDS
GTTAAACTCTAAGCAAGTAA+AGG 0.515924 2:-45677112 None:intergenic
TTCTAGTGGCACACGTATGG+TGG 0.520065 2:+45676629 MsG0280009118.01.T01:CDS
AGCTGTGGAACCTATTTCAA+TGG 0.524917 2:+45676932 MsG0280009118.01.T01:CDS
TCCACGACGGTATCCGAAAA+GGG 0.528553 2:-45676655 None:intergenic
CCATTCCCACTACTTGGCAT+TGG 0.536474 2:-45676969 None:intergenic
GAACCTATTTCAATGGGTGC+TGG 0.538761 2:+45676939 MsG0280009118.01.T01:CDS
GCTGTGGAACCTATTTCAAT+GGG 0.541783 2:+45676933 MsG0280009118.01.T01:CDS
TGGAGTGGTGAGGATTGCAT+TGG 0.543210 2:+45676776 MsG0280009118.01.T01:CDS
CAGTTCTAGTGGCACACGTA+TGG 0.547741 2:+45676626 MsG0280009118.01.T01:CDS
GGGAATGGAGGTGAGTTTGA+AGG 0.549280 2:+45676984 MsG0280009118.01.T01:CDS
GCATTGGAGTGTGAGAAGAA+AGG 0.552731 2:+45676792 MsG0280009118.01.T01:CDS
CCAATGCCAAGTAGTGGGAA+TGG 0.559271 2:+45676969 MsG0280009118.01.T01:CDS
ATGAACCCTGTTGGTAGTGG+TGG 0.559559 2:+45677077 MsG0280009118.01.T01:CDS
AGAAGAGTCATGTGGTGACT+TGG 0.563167 2:-45676325 None:intergenic
TATGAGAGCTAAGTATGAGA+GGG 0.565675 2:+45677019 MsG0280009118.01.T01:CDS
CGTTCACTTCACAGTTCTAG+TGG 0.569271 2:+45676615 MsG0280009118.01.T01:CDS
ATGGAAATACCTTCTAGAGA+AGG 0.577098 2:-45676352 None:intergenic
ATATGAGAGCTAAGTATGAG+AGG 0.585257 2:+45677018 MsG0280009118.01.T01:CDS
ATGATGAACCCTGTTGGTAG+TGG 0.586626 2:+45677074 MsG0280009118.01.T01:CDS
TTCTACATGATGAACCCTGT+TGG 0.588457 2:+45677068 MsG0280009118.01.T01:CDS
ATGGAAGATATTGAAAGCTG+TGG 0.589180 2:+45676917 MsG0280009118.01.T01:CDS
ATGAATGTGGATCCGAGGAA+TGG 0.598910 2:+45676898 MsG0280009118.01.T01:CDS
TAGGGAAATGGCTTCTGGAG+TGG 0.612183 2:+45676761 MsG0280009118.01.T01:CDS
TCTAGTGGCACACGTATGGT+GGG 0.631207 2:+45676630 MsG0280009118.01.T01:CDS
AAGGAGAAAGAAGAGTCATG+TGG 0.646233 2:-45676333 None:intergenic
TAGGCATGAATGTGGATCCG+AGG 0.652974 2:+45676893 MsG0280009118.01.T01:CDS
ATGCCAAGTAGTGGGAATGG+AGG 0.656575 2:+45676972 MsG0280009118.01.T01:CDS
TGTTGCCAATGCCAAGTAGT+GGG 0.657243 2:+45676964 MsG0280009118.01.T01:CDS
CAGGACCACCACTACCAACA+GGG 0.658555 2:-45677082 None:intergenic
AAATGGCTTCTGGAGTGGTG+AGG 0.662532 2:+45676766 MsG0280009118.01.T01:CDS
GTGTTGCCAATGCCAAGTAG+TGG 0.663696 2:+45676963 MsG0280009118.01.T01:CDS
TATGCATATAGGCATGAATG+TGG 0.680421 2:+45676885 MsG0280009118.01.T01:CDS
TGTTGGTGGTGAGAAAGAAG+AGG 0.693894 2:+45676467 MsG0280009118.01.T01:CDS
CAAAATGGACATGTCCACGA+CGG 0.712176 2:-45676668 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTAATGGAAGAAAATG+TGG + Chr2:45676861-45676880 MsG0280009118.01.T01:CDS 25.0%
CTAGCTCAATTAAGAATGTA+GGG - Chr2:45676719-45676738 None:intergenic 30.0%
GAAAATGTGGTTATGCATAT+AGG + Chr2:45676874-45676893 MsG0280009118.01.T01:CDS 30.0%
GTATTTCCATTGGAAAAAGA+AGG + Chr2:45676365-45676384 MsG0280009118.01.T01:CDS 30.0%
TTACAATAAGACCTCCAAAT+TGG - Chr2:45676849-45676868 None:intergenic 30.0%
TTTCAATATCTTCCATTCCT+CGG - Chr2:45676913-45676932 None:intergenic 30.0%
!! ATTTGGAGGTCTTATTGTAA+TGG + Chr2:45676849-45676868 MsG0280009118.01.T01:CDS 30.0%
AGTCATCTTGGAAAGCAAAA+TGG - Chr2:45676686-45676705 None:intergenic 35.0%
AGTTGCTTGAAGAACCAATT+TGG + Chr2:45676832-45676851 MsG0280009118.01.T01:CDS 35.0%
ATATGAGAGCTAAGTATGAG+AGG + Chr2:45677018-45677037 MsG0280009118.01.T01:CDS 35.0%
ATGGAAGATATTGAAAGCTG+TGG + Chr2:45676917-45676936 MsG0280009118.01.T01:CDS 35.0%
CAGCAATTTCTAATGGCATT+TGG - Chr2:45676530-45676549 None:intergenic 35.0%
CTAAGTATGAGAGGGTTATT+GGG + Chr2:45677027-45677046 MsG0280009118.01.T01:CDS 35.0%
GCTAGCTCAATTAAGAATGT+AGG - Chr2:45676720-45676739 None:intergenic 35.0%
TATGAGAGCTAAGTATGAGA+GGG + Chr2:45677019-45677038 MsG0280009118.01.T01:CDS 35.0%
TATGCATATAGGCATGAATG+TGG + Chr2:45676885-45676904 MsG0280009118.01.T01:CDS 35.0%
TCAAGAGCAGCAATTTCTAA+TGG - Chr2:45676537-45676556 None:intergenic 35.0%
! ATGGAAATACCTTCTAGAGA+AGG - Chr2:45676355-45676374 None:intergenic 35.0%
! GATGATGATGATGATGATGT+TGG + Chr2:45676450-45676469 MsG0280009118.01.T01:CDS 35.0%
! TTCATCATCACATTTTCCTG+AGG + Chr2:45676428-45676447 MsG0280009118.01.T01:CDS 35.0%
!! TCTCTAGAAGGTATTTCCAT+TGG + Chr2:45676355-45676374 MsG0280009118.01.T01:CDS 35.0%
!!! AAGTGTTTTGGTTAGGGAAA+TGG + Chr2:45676749-45676768 MsG0280009118.01.T01:CDS 35.0%
!!! ACCAACAAGTGTTTTGGTTA+GGG + Chr2:45676743-45676762 MsG0280009118.01.T01:CDS 35.0%
AAGGAGAAAGAAGAGTCATG+TGG - Chr2:45676336-45676355 None:intergenic 40.0%
AGCTGTGGAACCTATTTCAA+TGG + Chr2:45676932-45676951 MsG0280009118.01.T01:CDS 40.0%
CCATTGGAAAAAGAAGGCTT+TGG + Chr2:45676371-45676390 MsG0280009118.01.T01:CDS 40.0%
CTTCTTTCTCCTTCTCTAGA+AGG + Chr2:45676343-45676362 MsG0280009118.01.T01:CDS 40.0%
GCTAAGTATGAGAGGGTTAT+TGG + Chr2:45677026-45677045 MsG0280009118.01.T01:CDS 40.0%
GCTGTGGAACCTATTTCAAT+GGG + Chr2:45676933-45676952 MsG0280009118.01.T01:CDS 40.0%
TCATCATCATCATCATCCTC+AGG - Chr2:45676447-45676466 None:intergenic 40.0%
TCCCTAACCAAAACACTTGT+TGG - Chr2:45676747-45676766 None:intergenic 40.0%
TGCTTGAAGAACCAATTTGG+AGG + Chr2:45676835-45676854 MsG0280009118.01.T01:CDS 40.0%
TTCTACATGATGAACCCTGT+TGG + Chr2:45677068-45677087 MsG0280009118.01.T01:CDS 40.0%
! AGCAACACCAACAAGTGTTT+TGG + Chr2:45676737-45676756 MsG0280009118.01.T01:CDS 40.0%
! CCAAAGCCTTCTTTTTCCAA+TGG - Chr2:45676374-45676393 None:intergenic 40.0%
! GATGATGATGATGATGTTGG+TGG + Chr2:45676453-45676472 MsG0280009118.01.T01:CDS 40.0%
!!! CACCAACAAGTGTTTTGGTT+AGG + Chr2:45676742-45676761 MsG0280009118.01.T01:CDS 40.0%
ACACCAGCACCCATTGAAAT+AGG - Chr2:45676945-45676964 None:intergenic 45.0%
AGAAGAGTCATGTGGTGACT+TGG - Chr2:45676328-45676347 None:intergenic 45.0%
AGCAAGTAAAGGCAGAGTTC+AGG - Chr2:45677104-45677123 None:intergenic 45.0%
ATGAATGTGGATCCGAGGAA+TGG + Chr2:45676898-45676917 MsG0280009118.01.T01:CDS 45.0%
CAAAATGGACATGTCCACGA+CGG - Chr2:45676671-45676690 None:intergenic 45.0%
GAACCTATTTCAATGGGTGC+TGG + Chr2:45676939-45676958 MsG0280009118.01.T01:CDS 45.0%
GCATTGGAGTGTGAGAAGAA+AGG + Chr2:45676792-45676811 MsG0280009118.01.T01:CDS 45.0%
GGCTCAACTTTGAGTCATCT+TGG - Chr2:45676698-45676717 None:intergenic 45.0%
TGGAGTGTGAGAAGAAAGGA+GGG + Chr2:45676796-45676815 MsG0280009118.01.T01:CDS 45.0%
TGTTGCCAATGCCAAGTAGT+GGG + Chr2:45676964-45676983 MsG0280009118.01.T01:CDS 45.0%
TTGGAGTGTGAGAAGAAAGG+AGG + Chr2:45676795-45676814 MsG0280009118.01.T01:CDS 45.0%
TTGGTTAGGGAAATGGCTTC+TGG + Chr2:45676756-45676775 MsG0280009118.01.T01:CDS 45.0%
! ATGATGAACCCTGTTGGTAG+TGG + Chr2:45677074-45677093 MsG0280009118.01.T01:CDS 45.0%
! CGTTCACTTCACAGTTCTAG+TGG + Chr2:45676615-45676634 MsG0280009118.01.T01:CDS 45.0%
!! GGCTTTGGATGAAGATGATG+AGG + Chr2:45676386-45676405 MsG0280009118.01.T01:CDS 45.0%
!! TGTTGGTGGTGAGAAAGAAG+AGG + Chr2:45676467-45676486 MsG0280009118.01.T01:CDS 45.0%
CCATTCCCACTACTTGGCAT+TGG - Chr2:45676972-45676991 None:intergenic 50.0%
GGGAATGGAGGTGAGTTTGA+AGG + Chr2:45676984-45677003 MsG0280009118.01.T01:CDS 50.0%
GTGTTGCCAATGCCAAGTAG+TGG + Chr2:45676963-45676982 MsG0280009118.01.T01:CDS 50.0%
TAGGCATGAATGTGGATCCG+AGG + Chr2:45676893-45676912 MsG0280009118.01.T01:CDS 50.0%
TCACCTCCATTCCCACTACT+TGG - Chr2:45676978-45676997 None:intergenic 50.0%
TCCACGACGGTATCCGAAAA+GGG - Chr2:45676658-45676677 None:intergenic 50.0%
TCTAGTGGCACACGTATGGT+GGG + Chr2:45676630-45676649 MsG0280009118.01.T01:CDS 50.0%
TGGAGTGGTGAGGATTGCAT+TGG + Chr2:45676776-45676795 MsG0280009118.01.T01:CDS 50.0%
TTCTAGTGGCACACGTATGG+TGG + Chr2:45676629-45676648 MsG0280009118.01.T01:CDS 50.0%
! ATGCCAAGTAGTGGGAATGG+AGG + Chr2:45676972-45676991 MsG0280009118.01.T01:CDS 50.0%
! CAGTTCTAGTGGCACACGTA+TGG + Chr2:45676626-45676645 MsG0280009118.01.T01:CDS 50.0%
! CCAATGCCAAGTAGTGGGAA+TGG + Chr2:45676969-45676988 MsG0280009118.01.T01:CDS 50.0%
! TAGGGAAATGGCTTCTGGAG+TGG + Chr2:45676761-45676780 MsG0280009118.01.T01:CDS 50.0%
!! AAATGGCTTCTGGAGTGGTG+AGG + Chr2:45676766-45676785 MsG0280009118.01.T01:CDS 50.0%
!! ATGAACCCTGTTGGTAGTGG+TGG + Chr2:45677077-45677096 MsG0280009118.01.T01:CDS 50.0%
CAGGACCACCACTACCAACA+GGG - Chr2:45677085-45677104 None:intergenic 55.0%
GTCCACGACGGTATCCGAAA+AGG - Chr2:45676659-45676678 None:intergenic 55.0%
TCAGGACCACCACTACCAAC+AGG - Chr2:45677086-45677105 None:intergenic 55.0%
! ACCCTTTTCGGATACCGTCG+TGG + Chr2:45676654-45676673 MsG0280009118.01.T01:CDS 55.0%
!!! CGTATGGTGGGCACCCTTTT+CGG + Chr2:45676642-45676661 MsG0280009118.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr2 gene 45676318 45677133 45676318 ID=MsG0280009118.01;Name=MsG0280009118.01
Chr2 mRNA 45676318 45677133 45676318 ID=MsG0280009118.01.T01;Parent=MsG0280009118.01;Name=MsG0280009118.01.T01;_AED=0.47;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|271
Chr2 exon 45676318 45677133 45676318 ID=MsG0280009118.01.T01:exon:438;Parent=MsG0280009118.01.T01
Chr2 CDS 45676318 45677133 45676318 ID=MsG0280009118.01.T01:cds;Parent=MsG0280009118.01.T01
Gene Sequence

>MsG0280009118.01.T01

ATGGCAGCCAAGTCACCACATGACTCTTCTTTCTCCTTCTCTAGAAGGTATTTCCATTGGAAAAAGAAGGCTTTGGATGAAGATGATGAGGAAGAAATACTCAATATAAGTTCATCATCACATTTTCCTGAGGATGATGATGATGATGATGTTGGTGGTGAGAAAGAAGAGGTTCATCATCATCGACATCATCATAATCATTTAAGAATCCAAATGCCATTAGAAATTGCTGCTCTTGAAAAGAAGAAGAATTCAAAATCAAAGTTCAAGTCAGCACTAACAATTCTCACAAAGAATCGTTCACTTCACAGTTCTAGTGGCACACGTATGGTGGGCACCCTTTTCGGATACCGTCGTGGACATGTCCATTTTGCTTTCCAAGATGACTCAAAGTTGAGCCCTACATTCTTAATTGAGCTAGCAACACCAACAAGTGTTTTGGTTAGGGAAATGGCTTCTGGAGTGGTGAGGATTGCATTGGAGTGTGAGAAGAAAGGAGGGAGAAAGAGTTTGAAGTTGCTTGAAGAACCAATTTGGAGGTCTTATTGTAATGGAAGAAAATGTGGTTATGCATATAGGCATGAATGTGGATCCGAGGAATGGAAGATATTGAAAGCTGTGGAACCTATTTCAATGGGTGCTGGTGTGTTGCCAATGCCAAGTAGTGGGAATGGAGGTGAGTTTGAAGGTGAGCTCATGTATATGAGAGCTAAGTATGAGAGGGTTATTGGGTCTAAAGATTCTGAAGCTTTCTACATGATGAACCCTGTTGGTAGTGGTGGTCCTGAACTCTGCCTTTACTTGCTTAGAGTTTAA

Protein sequence

>MsG0280009118.01.T01

MAAKSPHDSSFSFSRRYFHWKKKALDEDDEEEILNISSSSHFPEDDDDDDVGGEKEEVHHHRHHHNHLRIQMPLEIAALEKKKNSKSKFKSALTILTKNRSLHSSSGTRMVGTLFGYRRGHVHFAFQDDSKLSPTFLIELATPTSVLVREMASGVVRIALECEKKGGRKSLKLLEEPIWRSYCNGRKCGYAYRHECGSEEWKILKAVEPISMGAGVLPMPSSGNGGEFEGELMYMRAKYERVIGSKDSEAFYMMNPVGSGGPELCLYLLRV*