Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011134.01.T01 | XP_003597545.2 | 93.939 | 165 | 10 | 0 | 1 | 165 | 1 | 165 | 5.59E-110 | 320 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011134.01.T01 | G7IS16 | 93.939 | 165 | 10 | 0 | 1 | 165 | 1 | 165 | 2.67e-110 | 320 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000218.01 | MsG0280011134.01 | 0.837851 | 4.071985e-57 | 5.357552e-54 |
| MsG0180000354.01 | MsG0280011134.01 | -0.800727 | 1.258087e-48 | 5.901066e-46 |
| MsG0180000355.01 | MsG0280011134.01 | -0.818828 | 1.598521e-52 | 1.209521e-49 |
| MsG0180000400.01 | MsG0280011134.01 | 0.813302 | 2.742049e-51 | 1.784018e-48 |
| MsG0180000509.01 | MsG0280011134.01 | -0.816908 | 4.339434e-52 | 3.114622e-49 |
| MsG0180003353.01 | MsG0280011134.01 | -0.806987 | 6.299565e-50 | 3.467791e-47 |
| MsG0180003821.01 | MsG0280011134.01 | -0.808828 | 2.556127e-50 | 1.476488e-47 |
| MsG0180004493.01 | MsG0280011134.01 | -0.837048 | 6.541756e-57 | 8.396517e-54 |
| MsG0180005429.01 | MsG0280011134.01 | -0.801212 | 1.001577e-48 | 4.756006e-46 |
| MsG0180006104.01 | MsG0280011134.01 | -0.815677 | 8.177591e-52 | 5.675321e-49 |
| MsG0280011134.01 | MsG0380017094.01 | 0.824574 | 7.497930e-54 | 6.667886e-51 |
| MsG0280011134.01 | MsG0480019984.01 | 0.823240 | 1.540568e-53 | 1.319065e-50 |
| MsG0280011134.01 | MsG0480022401.01 | -0.815743 | 7.907341e-52 | 5.497828e-49 |
| MsG0280011134.01 | MsG0480023384.01 | -0.816738 | 4.737856e-52 | 3.384747e-49 |
| MsG0280011134.01 | MsG0480023400.01 | 0.815555 | 8.706327e-52 | 6.021847e-49 |
| MsG0280011134.01 | MsG0580024930.01 | 0.807808 | 4.217866e-50 | 2.371997e-47 |
| MsG0280011134.01 | MsG0580028613.01 | 0.814925 | 1.201630e-51 | 8.168964e-49 |
| MsG0280011134.01 | MsG0580029919.01 | -0.811481 | 6.852966e-51 | 4.246883e-48 |
| MsG0280011134.01 | MsG0680031805.01 | 0.802253 | 6.125454e-49 | 2.986448e-46 |
| MsG0280011134.01 | MsG0780041482.01 | -0.823832 | 1.119975e-53 | 9.751902e-51 |
| MsG0280011134.01 | MsG0780041729.01 | 0.805001 | 1.648107e-49 | 8.621877e-47 |
| MsG0280011134.01 | MsG0780041734.01 | 0.811627 | 6.369803e-51 | 3.962421e-48 |
| MsG0280011134.01 | MsG0880046867.01 | -0.803552 | 3.300692e-49 | 1.663624e-46 |
| MsG0280007165.01 | MsG0280011134.01 | 0.817538 | 3.130366e-52 | 2.285991e-49 |
| MsG0280009685.01 | MsG0280011134.01 | -0.814397 | 1.572817e-51 | 1.054235e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011134.01.T01 | MTR_2g099440 | 93.939 | 165 | 10 | 0 | 1 | 165 | 1 | 165 | 6.77e-114 | 320 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280011134.01.T01 | AT2G43540 | 48.235 | 170 | 80 | 3 | 1 | 164 | 1 | 168 | 7.41e-43 | 140 |
Find 51 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAGTTCCTGTTGGTGTTGC+TGG | 0.251042 | 2:+80028729 | MsG0280011134.01.T01:CDS |
| CTTCGAAGGTGTGTTGTGTT+AGG | 0.256702 | 2:-80028461 | None:intergenic |
| TCTTTACACCGACGCTGTTT+TGG | 0.298710 | 2:+80028680 | MsG0280011134.01.T01:CDS |
| CGACAGCGTCCTTCGACTTC+CGG | 0.324677 | 2:+80028635 | MsG0280011134.01.T01:CDS |
| GTCGCAGGCTGAGTTGAATT+CGG | 0.348824 | 2:-80028415 | None:intergenic |
| TCGCAGGCTGAGTTGAATTC+GGG | 0.354900 | 2:-80028414 | None:intergenic |
| GACGCTGTCGCGGTTGTGAT+CGG | 0.362382 | 2:-80028622 | None:intergenic |
| GATCTGGTCCTGTGCTGCTT+GGG | 0.387437 | 2:+80028778 | MsG0280011134.01.T01:CDS |
| ATTGGTGCTGTTGCAAGATC+TGG | 0.425634 | 2:+80028762 | MsG0280011134.01.T01:CDS |
| AGATCTGGTCCTGTGCTGCT+TGG | 0.427958 | 2:+80028777 | MsG0280011134.01.T01:CDS |
| TTCGAAGGTGTGTTGTGTTA+GGG | 0.436255 | 2:-80028460 | None:intergenic |
| ACGCTGTCGCGGTTGTGATC+GGG | 0.437599 | 2:-80028621 | None:intergenic |
| GGCGGAGTGCGGAGTCAACT+TGG | 0.443036 | 2:-80028587 | None:intergenic |
| GTTGCTGGGATCGTTGGAAT+TGG | 0.443473 | 2:+80028744 | MsG0280011134.01.T01:CDS |
| TGGGTTCGATCGGGAGGAGA+TGG | 0.444187 | 2:-80028567 | None:intergenic |
| ATGGGACCCAGTTGTGGATG+AGG | 0.463184 | 2:-80028548 | None:intergenic |
| ACGATCCCAGCAACACCAAC+AGG | 0.463266 | 2:-80028735 | None:intergenic |
| TAGGGAGAGGCAGTGGATGA+AGG | 0.474118 | 2:-80028442 | None:intergenic |
| GAGTTCCTGTTGGTGTTGCT+GGG | 0.475369 | 2:+80028730 | MsG0280011134.01.T01:CDS |
| GGTGGTTGGTGAGGAGAGCG+TGG | 0.479032 | 2:-80028524 | None:intergenic |
| CGAGCCCATTCCTTGAAAAC+CGG | 0.493356 | 2:-80028654 | None:intergenic |
| GTGGATGAAGGTTGTGTCGC+AGG | 0.509502 | 2:-80028430 | None:intergenic |
| GGAGTCAACTTGGGTTCGAT+CGG | 0.509646 | 2:-80028577 | None:intergenic |
| GAGTCAACTTGGGTTCGATC+GGG | 0.510610 | 2:-80028576 | None:intergenic |
| GTTGGTGTTGCTGGGATCGT+TGG | 0.512435 | 2:+80028738 | MsG0280011134.01.T01:CDS |
| TTTGTTGGAGCTTACTCGAT+CGG | 0.517892 | 2:+80028804 | MsG0280011134.01.T01:CDS |
| GGGTTCGATCGGGAGGAGAT+GGG | 0.520000 | 2:-80028566 | None:intergenic |
| AGCGTGGATTTGATCGGAAG+CGG | 0.524433 | 2:-80028508 | None:intergenic |
| GTTGTGTTAGGGAGAGGCAG+TGG | 0.528825 | 2:-80028449 | None:intergenic |
| ACAAAACTCCCAAGCAGCAC+AGG | 0.530149 | 2:-80028786 | None:intergenic |
| CGCGGTTGTGATCGGGTGGA+AGG | 0.531290 | 2:-80028614 | None:intergenic |
| TGGGACCCAGTTGTGGATGA+GGG | 0.534470 | 2:-80028547 | None:intergenic |
| TGTGATCGGGTGGAAGGACA+CGG | 0.537395 | 2:-80028608 | None:intergenic |
| GGACCCAGTTGTGGATGAGG+GGG | 0.538461 | 2:-80028545 | None:intergenic |
| GAGGAGATGGGACCCAGTTG+TGG | 0.541506 | 2:-80028554 | None:intergenic |
| AGGTGTGTTGTGTTAGGGAG+AGG | 0.553651 | 2:-80028455 | None:intergenic |
| TGGAAGGACACGGCGGAGTG+CGG | 0.555448 | 2:-80028598 | None:intergenic |
| GAGGAGAGCGTGGATTTGAT+CGG | 0.559071 | 2:-80028514 | None:intergenic |
| CGCAGGCTGAGTTGAATTCG+GGG | 0.568422 | 2:-80028413 | None:intergenic |
| GCGGAGTGCGGAGTCAACTT+GGG | 0.578376 | 2:-80028586 | None:intergenic |
| CCTTGAAAACCGGAAGTCGA+AGG | 0.578517 | 2:-80028644 | None:intergenic |
| GGGACCCAGTTGTGGATGAG+GGG | 0.591665 | 2:-80028546 | None:intergenic |
| CTAACACAACACACCTTCGA+AGG | 0.594264 | 2:+80028462 | MsG0280011134.01.T01:CDS |
| CTGTCGCGGTTGTGATCGGG+TGG | 0.596032 | 2:-80028618 | None:intergenic |
| TCAACTTGGGTTCGATCGGG+AGG | 0.602242 | 2:-80028573 | None:intergenic |
| GAAGTCGAAGGACGCTGTCG+CGG | 0.617152 | 2:-80028632 | None:intergenic |
| GTAGGGTAAGACTCCTTCGA+AGG | 0.634603 | 2:-80028475 | None:intergenic |
| AGCAGAAGCCAAAACAGCGT+CGG | 0.649459 | 2:-80028688 | None:intergenic |
| TTGATTGTAAGAGTTCCTGT+TGG | 0.666947 | 2:+80028720 | MsG0280011134.01.T01:CDS |
| GCAGGCTGAGTTGAATTCGG+GGG | 0.679036 | 2:-80028412 | None:intergenic |
| GATCGGGTGGAAGGACACGG+CGG | 0.789521 | 2:-80028605 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTGCTCTTTCTATTTTTCT+TGG | + | Chr2:80028828-80028847 | MsG0280011134.01.T01:CDS | 30.0% |
| TTGATTGTAAGAGTTCCTGT+TGG | + | Chr2:80028720-80028739 | MsG0280011134.01.T01:CDS | 35.0% | |
| TTTGTTGGAGCTTACTCGAT+CGG | + | Chr2:80028804-80028823 | MsG0280011134.01.T01:CDS | 40.0% | |
| ! | TTCGAAGGTGTGTTGTGTTA+GGG | - | Chr2:80028463-80028482 | None:intergenic | 40.0% |
| !! | ACTTCCGGTTTTCAAGGAAT+GGG | + | Chr2:80028650-80028669 | MsG0280011134.01.T01:CDS | 40.0% |
| CTAACACAACACACCTTCGA+AGG | + | Chr2:80028462-80028481 | MsG0280011134.01.T01:CDS | 45.0% | |
| ! | CTTCGAAGGTGTGTTGTGTT+AGG | - | Chr2:80028464-80028483 | None:intergenic | 45.0% |
| ! | TCTTTACACCGACGCTGTTT+TGG | + | Chr2:80028680-80028699 | MsG0280011134.01.T01:CDS | 45.0% |
| !! | ATTGGTGCTGTTGCAAGATC+TGG | + | Chr2:80028762-80028781 | MsG0280011134.01.T01:CDS | 45.0% |
| !! | GACTTCCGGTTTTCAAGGAA+TGG | + | Chr2:80028649-80028668 | MsG0280011134.01.T01:CDS | 45.0% |
| !!! | GAAGCGGTTTTGAGTTGGTA+GGG | - | Chr2:80028495-80028514 | None:intergenic | 45.0% |
| ACAAAACTCCCAAGCAGCAC+AGG | - | Chr2:80028789-80028808 | None:intergenic | 50.0% | |
| AGCAGAAGCCAAAACAGCGT+CGG | - | Chr2:80028691-80028710 | None:intergenic | 50.0% | |
| AGCGTGGATTTGATCGGAAG+CGG | - | Chr2:80028511-80028530 | None:intergenic | 50.0% | |
| AGGTGTGTTGTGTTAGGGAG+AGG | - | Chr2:80028458-80028477 | None:intergenic | 50.0% | |
| AGTTGAATTCGGGGGAGGTT+TGG | - | Chr2:80028407-80028426 | None:intergenic | 50.0% | |
| CCTTGAAAACCGGAAGTCGA+AGG | - | Chr2:80028647-80028666 | None:intergenic | 50.0% | |
| CGAGCCCATTCCTTGAAAAC+CGG | - | Chr2:80028657-80028676 | None:intergenic | 50.0% | |
| GAGTCAACTTGGGTTCGATC+GGG | - | Chr2:80028579-80028598 | None:intergenic | 50.0% | |
| GGAGTCAACTTGGGTTCGAT+CGG | - | Chr2:80028580-80028599 | None:intergenic | 50.0% | |
| GTAGGGTAAGACTCCTTCGA+AGG | - | Chr2:80028478-80028497 | None:intergenic | 50.0% | |
| GTTGCTGGGATCGTTGGAAT+TGG | + | Chr2:80028744-80028763 | MsG0280011134.01.T01:CDS | 50.0% | |
| ! | CCTTCGACTTCCGGTTTTCA+AGG | + | Chr2:80028644-80028663 | MsG0280011134.01.T01:CDS | 50.0% |
| ! | GAGGAGAGCGTGGATTTGAT+CGG | - | Chr2:80028517-80028536 | None:intergenic | 50.0% |
| ! | GTCGCAGGCTGAGTTGAATT+CGG | - | Chr2:80028418-80028437 | None:intergenic | 50.0% |
| ! | TCGCAGGCTGAGTTGAATTC+GGG | - | Chr2:80028417-80028436 | None:intergenic | 50.0% |
| !! | AGAGTTCCTGTTGGTGTTGC+TGG | + | Chr2:80028729-80028748 | MsG0280011134.01.T01:CDS | 50.0% |
| !! | CGGTTTTCAAGGAATGGGCT+CGG | + | Chr2:80028655-80028674 | MsG0280011134.01.T01:CDS | 50.0% |
| !! | GAGTTCCTGTTGGTGTTGCT+GGG | + | Chr2:80028730-80028749 | MsG0280011134.01.T01:CDS | 50.0% |
| !!! | GATCGGAAGCGGTTTTGAGT+TGG | - | Chr2:80028500-80028519 | None:intergenic | 50.0% |
| !!! | GGAAGCGGTTTTGAGTTGGT+AGG | - | Chr2:80028496-80028515 | None:intergenic | 50.0% |
| !!! | GTGCTGCTTGGGAGTTTTGT+TGG | + | Chr2:80028789-80028808 | MsG0280011134.01.T01:CDS | 50.0% |
| ACGATCCCAGCAACACCAAC+AGG | - | Chr2:80028738-80028757 | None:intergenic | 55.0% | |
| ATGGGACCCAGTTGTGGATG+AGG | - | Chr2:80028551-80028570 | None:intergenic | 55.0% | |
| GTGGATGAAGGTTGTGTCGC+AGG | - | Chr2:80028433-80028452 | None:intergenic | 55.0% | |
| GTTGTGTTAGGGAGAGGCAG+TGG | - | Chr2:80028452-80028471 | None:intergenic | 55.0% | |
| TAGGGAGAGGCAGTGGATGA+AGG | - | Chr2:80028445-80028464 | None:intergenic | 55.0% | |
| TCAACTTGGGTTCGATCGGG+AGG | - | Chr2:80028576-80028595 | None:intergenic | 55.0% | |
| TGGGACCCAGTTGTGGATGA+GGG | - | Chr2:80028550-80028569 | None:intergenic | 55.0% | |
| TGTGATCGGGTGGAAGGACA+CGG | - | Chr2:80028611-80028630 | None:intergenic | 55.0% | |
| ! | AGATCTGGTCCTGTGCTGCT+TGG | + | Chr2:80028777-80028796 | MsG0280011134.01.T01:CDS | 55.0% |
| ! | CGCAGGCTGAGTTGAATTCG+GGG | - | Chr2:80028416-80028435 | None:intergenic | 55.0% |
| ! | GCAGGCTGAGTTGAATTCGG+GGG | - | Chr2:80028415-80028434 | None:intergenic | 55.0% |
| !! | GATCTGGTCCTGTGCTGCTT+GGG | + | Chr2:80028778-80028797 | MsG0280011134.01.T01:CDS | 55.0% |
| !! | GTTGGTGTTGCTGGGATCGT+TGG | + | Chr2:80028738-80028757 | MsG0280011134.01.T01:CDS | 55.0% |
| !!! | GACGCTGTTTTGGCTTCTGC+TGG | + | Chr2:80028690-80028709 | MsG0280011134.01.T01:CDS | 55.0% |
| ACCACCCCCTCATCCACAAC+TGG | + | Chr2:80028541-80028560 | MsG0280011134.01.T01:CDS | 60.0% | |
| ACGCTGTCGCGGTTGTGATC+GGG | - | Chr2:80028624-80028643 | None:intergenic | 60.0% | |
| CCACCCCCTCATCCACAACT+GGG | + | Chr2:80028542-80028561 | MsG0280011134.01.T01:CDS | 60.0% | |
| CGACAGCGTCCTTCGACTTC+CGG | + | Chr2:80028635-80028654 | MsG0280011134.01.T01:CDS | 60.0% | |
| GAAGTCGAAGGACGCTGTCG+CGG | - | Chr2:80028635-80028654 | None:intergenic | 60.0% | |
| GACGCTGTCGCGGTTGTGAT+CGG | - | Chr2:80028625-80028644 | None:intergenic | 60.0% | |
| GAGGAGATGGGACCCAGTTG+TGG | - | Chr2:80028557-80028576 | None:intergenic | 60.0% | |
| GCGGAGTGCGGAGTCAACTT+GGG | - | Chr2:80028589-80028608 | None:intergenic | 60.0% | |
| GGACCCAGTTGTGGATGAGG+GGG | - | Chr2:80028548-80028567 | None:intergenic | 60.0% | |
| GGGACCCAGTTGTGGATGAG+GGG | - | Chr2:80028549-80028568 | None:intergenic | 60.0% | |
| GGGTTCGATCGGGAGGAGAT+GGG | - | Chr2:80028569-80028588 | None:intergenic | 60.0% | |
| GTTGTGGATGAGGGGGTGGT+TGG | - | Chr2:80028541-80028560 | None:intergenic | 60.0% | |
| TGGGTTCGATCGGGAGGAGA+TGG | - | Chr2:80028570-80028589 | None:intergenic | 60.0% | |
| ! | GGCTGAGTTGAATTCGGGGG+AGG | - | Chr2:80028412-80028431 | None:intergenic | 60.0% |
| CCCAGTTGTGGATGAGGGGG+TGG | - | Chr2:80028545-80028564 | None:intergenic | 65.0% | |
| CGCGGTTGTGATCGGGTGGA+AGG | - | Chr2:80028617-80028636 | None:intergenic | 65.0% | |
| CTGTCGCGGTTGTGATCGGG+TGG | - | Chr2:80028621-80028640 | None:intergenic | 65.0% | |
| GATCGGGTGGAAGGACACGG+CGG | - | Chr2:80028608-80028627 | None:intergenic | 65.0% | |
| GGCGGAGTGCGGAGTCAACT+TGG | - | Chr2:80028590-80028609 | None:intergenic | 65.0% | |
| TGGAAGGACACGGCGGAGTG+CGG | - | Chr2:80028601-80028620 | None:intergenic | 65.0% | |
| ! | GGATGAGGGGGTGGTTGGTG+AGG | - | Chr2:80028536-80028555 | None:intergenic | 65.0% |
| !! | GGTGGTTGGTGAGGAGAGCG+TGG | - | Chr2:80028527-80028546 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 80028366 | 80028863 | 80028366 | ID=MsG0280011134.01;Name=MsG0280011134.01 |
| Chr2 | mRNA | 80028366 | 80028863 | 80028366 | ID=MsG0280011134.01.T01;Parent=MsG0280011134.01;Name=MsG0280011134.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|165 |
| Chr2 | exon | 80028366 | 80028863 | 80028366 | ID=MsG0280011134.01.T01:exon:21505;Parent=MsG0280011134.01.T01 |
| Chr2 | CDS | 80028366 | 80028863 | 80028366 | ID=MsG0280011134.01.T01:cds;Parent=MsG0280011134.01.T01 |
| Gene Sequence |
| Protein sequence |
