Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007909.01.T01 | XP_003594831.1 | 95.57 | 158 | 7 | 0 | 1 | 158 | 1 | 158 | 6.70E-105 | 306 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007909.01.T01 | P27047 | 86.076 | 158 | 22 | 0 | 1 | 158 | 1 | 158 | 4.35E-95 | 275 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007909.01.T01 | G7IMY7 | 95.570 | 158 | 7 | 0 | 1 | 158 | 1 | 158 | 3.20e-105 | 306 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003320.01 | MsG0280007909.01 | 0.823603 | 1.266999e-53 | 1.096057e-50 |
| MsG0180003322.01 | MsG0280007909.01 | 0.815547 | 8.740911e-52 | 6.044500e-49 |
| MsG0180003604.01 | MsG0280007909.01 | 0.814146 | 1.787229e-51 | 1.189761e-48 |
| MsG0180005648.01 | MsG0280007909.01 | 0.805282 | 1.439572e-49 | 7.585225e-47 |
| MsG0280007906.01 | MsG0280007909.01 | 0.843578 | 1.285687e-58 | 2.020573e-55 |
| MsG0280007908.01 | MsG0280007909.01 | 0.847724 | 9.649100e-60 | 1.730761e-56 |
| MsG0280007909.01 | MsG0280007911.01 | 0.805570 | 1.252575e-49 | 6.649109e-47 |
| MsG0280007909.01 | MsG0280009232.01 | 0.820409 | 6.963482e-53 | 5.505784e-50 |
| MsG0280007909.01 | MsG0280010005.01 | 0.811719 | 6.083110e-51 | 3.793478e-48 |
| MsG0280007909.01 | MsG0380013263.01 | 0.821852 | 3.238675e-53 | 2.665971e-50 |
| MsG0280007909.01 | MsG0380016086.01 | 0.805324 | 1.410228e-49 | 7.438986e-47 |
| MsG0280007909.01 | MsG0380017168.01 | 0.801403 | 9.155466e-49 | 4.368361e-46 |
| MsG0280007909.01 | MsG0480018521.01 | 0.825770 | 3.911253e-54 | 3.599015e-51 |
| MsG0280007909.01 | MsG0480018522.01 | 0.834427 | 3.016159e-56 | 3.578391e-53 |
| MsG0280007909.01 | MsG0480018622.01 | 0.801770 | 7.698359e-49 | 3.707285e-46 |
| MsG0280007909.01 | MsG0480019024.01 | 0.808342 | 3.247173e-50 | 1.852053e-47 |
| MsG0280007909.01 | MsG0480020118.01 | 0.806719 | 7.177319e-50 | 3.923705e-47 |
| MsG0280007909.01 | MsG0480020904.01 | 0.822899 | 1.850867e-53 | 1.569588e-50 |
| MsG0280007909.01 | MsG0580024207.01 | 0.811182 | 7.958136e-51 | 4.892920e-48 |
| MsG0280007909.01 | MsG0580024932.01 | 0.807633 | 4.594815e-50 | 2.572238e-47 |
| MsG0280007909.01 | MsG0580024990.01 | 0.814430 | 1.547092e-51 | 1.037878e-48 |
| MsG0280007909.01 | MsG0580025965.01 | 0.805705 | 1.173439e-49 | 6.251263e-47 |
| MsG0280007909.01 | MsG0580026521.01 | 0.811794 | 5.858265e-51 | 3.660540e-48 |
| MsG0280007909.01 | MsG0680031139.01 | 0.810859 | 9.345711e-51 | 5.697455e-48 |
| MsG0280007909.01 | MsG0680034974.01 | 0.826850 | 2.163253e-54 | 2.052769e-51 |
| MsG0280007909.01 | MsG0780036259.01 | 0.807093 | 5.982289e-50 | 3.302174e-47 |
| MsG0280007909.01 | MsG0780036472.01 | 0.829527 | 4.896421e-55 | 5.024281e-52 |
| MsG0280007909.01 | MsG0780038657.01 | 0.828056 | 1.111416e-54 | 1.092508e-51 |
| MsG0280007909.01 | MsG0780040615.01 | 0.821249 | 4.463055e-53 | 3.611835e-50 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007909.01.T01 | MTR_2g035120 | 95.570 | 158 | 7 | 0 | 1 | 158 | 1 | 158 | 8.12e-109 | 306 |
| MsG0280007909.01.T01 | MTR_2g035150 | 86.076 | 158 | 22 | 0 | 1 | 158 | 1 | 158 | 1.31e-96 | 276 |
| MsG0280007909.01.T01 | MTR_2g035100 | 83.439 | 157 | 26 | 0 | 1 | 157 | 1 | 157 | 2.03e-93 | 268 |
| MsG0280007909.01.T01 | MTR_2g035130 | 84.713 | 157 | 24 | 0 | 1 | 157 | 1 | 157 | 2.17e-93 | 268 |
| MsG0280007909.01.T01 | MTR_2g035105 | 82.166 | 157 | 28 | 0 | 1 | 157 | 1 | 157 | 4.06e-92 | 264 |
| MsG0280007909.01.T01 | MTR_6g033450 | 68.987 | 158 | 49 | 0 | 1 | 158 | 1 | 158 | 4.40e-76 | 224 |
| MsG0280007909.01.T01 | MTR_4g120970 | 68.987 | 158 | 49 | 0 | 1 | 158 | 1 | 158 | 4.40e-76 | 224 |
| MsG0280007909.01.T01 | MTR_2g035320 | 59.236 | 157 | 64 | 0 | 1 | 157 | 1 | 157 | 2.01e-63 | 192 |
| MsG0280007909.01.T01 | MTR_2g035190 | 58.599 | 157 | 65 | 0 | 1 | 157 | 1 | 157 | 3.74e-62 | 189 |
| MsG0280007909.01.T01 | MTR_2g035170 | 57.962 | 157 | 66 | 0 | 1 | 157 | 1 | 157 | 1.44e-59 | 182 |
| MsG0280007909.01.T01 | MTR_2g035210 | 56.688 | 157 | 68 | 0 | 1 | 157 | 1 | 157 | 6.16e-59 | 180 |
| MsG0280007909.01.T01 | MTR_2g035220 | 57.325 | 157 | 67 | 0 | 1 | 157 | 1 | 157 | 9.28e-58 | 177 |
| MsG0280007909.01.T01 | MTR_2g035320 | 58.586 | 99 | 41 | 0 | 59 | 157 | 24 | 122 | 3.07e-36 | 122 |
| MsG0280007909.01.T01 | MTR_4g120760 | 38.217 | 157 | 96 | 1 | 1 | 156 | 1 | 157 | 1.29e-35 | 121 |
| MsG0280007909.01.T01 | MTR_4g120760 | 38.217 | 157 | 96 | 1 | 1 | 156 | 1 | 157 | 1.32e-35 | 121 |
| MsG0280007909.01.T01 | MTR_2g435310 | 37.179 | 156 | 97 | 1 | 1 | 155 | 1 | 156 | 2.15e-35 | 120 |
| MsG0280007909.01.T01 | MTR_4g120950 | 45.679 | 162 | 81 | 5 | 1 | 158 | 1 | 159 | 4.34e-34 | 117 |
| MsG0280007909.01.T01 | MTR_4g120940 | 71.622 | 74 | 21 | 0 | 85 | 158 | 47 | 120 | 4.18e-30 | 106 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 42 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACGAAAGTGAGTTTCTTGA+TGG | 0.319436 | 2:-22789146 | None:intergenic |
| TTGATGACATCAACAACCTT+TGG | 0.332899 | 2:-22789085 | None:intergenic |
| GGCCCTTGAGGGTTACTGTT+TGG | 0.338813 | 2:+22789747 | MsG0280007909.01.T01:CDS |
| TATATGCTTTAGTTGTAATC+AGG | 0.341112 | 2:-22789775 | None:intergenic |
| CACTTTGTGTAAATCATACT+TGG | 0.346823 | 2:-22789503 | None:intergenic |
| TGATGACATCAACAACCTTT+GGG | 0.365094 | 2:-22789084 | None:intergenic |
| ACTTGGTAGATGATGCTAAC+TGG | 0.375253 | 2:+22789527 | MsG0280007909.01.T01:CDS |
| AGTGAGTTTCTTGATGGTTC+CGG | 0.377477 | 2:-22789140 | None:intergenic |
| GAAATCTTCTCTACTGTGTC+TGG | 0.378194 | 2:-22789586 | None:intergenic |
| CATCAACAACCTTTGGGATA+AGG | 0.380175 | 2:-22789078 | None:intergenic |
| AAGAAACTCACTTTCGTTGA+AGG | 0.384811 | 2:+22789151 | MsG0280007909.01.T01:CDS |
| AAAAGTATTGACATTGTTGA+AGG | 0.419014 | 2:+22789106 | MsG0280007909.01.T01:CDS |
| ATTGACATTGTTGAAGGAAA+TGG | 0.422286 | 2:+22789112 | MsG0280007909.01.T01:CDS |
| CTTGAAAAGACCTTCACCCT+TGG | 0.454441 | 2:-22789725 | None:intergenic |
| GCTTACAACTACAGCATAGT+TGG | 0.483791 | 2:+22789550 | MsG0280007909.01.T01:CDS |
| AAAGCTTTGTGAAGTGTAGC+AGG | 0.484835 | 2:-22789037 | None:intergenic |
| TTTGAAGCTAAATTGTCTGC+AGG | 0.489005 | 2:+22789610 | MsG0280007909.01.T01:CDS |
| CAAGTATGATTTACACAAAG+TGG | 0.491575 | 2:+22789504 | MsG0280007909.01.T01:CDS |
| TTTGCAATGGATCCTCCATT+CGG | 0.495837 | 2:-22789634 | None:intergenic |
| ACTAGGAGTAGCATCACCTT+TGG | 0.504856 | 2:-22789677 | None:intergenic |
| CTTGGTAGATGATGCTAACT+GGG | 0.513123 | 2:+22789528 | MsG0280007909.01.T01:CDS |
| TTTCACACTAAGCTTTGCAA+TGG | 0.532493 | 2:-22789647 | None:intergenic |
| CTCTCGAGGTCCTCTTCACT+AGG | 0.534215 | 2:-22789694 | None:intergenic |
| GTTGAAGGAAATGGTGGCGC+CGG | 0.546300 | 2:+22789121 | MsG0280007909.01.T01:CDS |
| TGATTTACACAAAGTGGACT+TGG | 0.556769 | 2:+22789510 | MsG0280007909.01.T01:CDS |
| CCTCGAGAGTGGCAAAGCCA+AGG | 0.566158 | 2:+22789708 | MsG0280007909.01.T01:CDS |
| AAGTGTAGCAGGAGCTACAA+TGG | 0.570662 | 2:-22789026 | None:intergenic |
| AGTGGCAAAGCCAAGGGTGA+AGG | 0.576358 | 2:+22789715 | MsG0280007909.01.T01:CDS |
| CTCGAGAGTGGCAAAGCCAA+GGG | 0.586680 | 2:+22789709 | MsG0280007909.01.T01:CDS |
| CCTTGGCTTTGCCACTCTCG+AGG | 0.588424 | 2:-22789708 | None:intergenic |
| AAATTGTCTGCAGGTCCGAA+TGG | 0.591580 | 2:+22789619 | MsG0280007909.01.T01:CDS |
| AGCAGGAGCTACAATGGAGG+TGG | 0.593310 | 2:-22789020 | None:intergenic |
| TACAACTACAGCATAGTTGG+AGG | 0.598501 | 2:+22789553 | MsG0280007909.01.T01:CDS |
| AGTGAAGAGGACCTCGAGAG+TGG | 0.617170 | 2:+22789697 | MsG0280007909.01.T01:CDS |
| TAGCCAAACAGTAACCCTCA+AGG | 0.618520 | 2:-22789750 | None:intergenic |
| TGCTGACAACCTTATCCCAA+AGG | 0.624297 | 2:+22789069 | MsG0280007909.01.T01:CDS |
| AGCCAAACAGTAACCCTCAA+GGG | 0.627034 | 2:-22789749 | None:intergenic |
| TGATGCTACTCCTAGTGAAG+AGG | 0.634756 | 2:+22789684 | MsG0280007909.01.T01:CDS |
| TTGTCTGCAGGTCCGAATGG+AGG | 0.646240 | 2:+22789622 | MsG0280007909.01.T01:CDS |
| AGTGTGAAATACTTCACCAA+AGG | 0.651010 | 2:+22789661 | MsG0280007909.01.T01:CDS |
| GACATTGTTGAAGGAAATGG+TGG | 0.661697 | 2:+22789115 | MsG0280007909.01.T01:CDS |
| TGTAGCAGGAGCTACAATGG+AGG | 0.722910 | 2:-22789023 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACCTAAATATATTAAGTAA+TGG | + | Chr2:22789430-22789449 | MsG0280007909.01.T01:intron | 15.0% |
| !! | AATCTTACTTATTAAGATTT+AGG | + | Chr2:22789210-22789229 | MsG0280007909.01.T01:intron | 15.0% |
| !! | ATCTTACTTATTAAGATTTA+GGG | + | Chr2:22789211-22789230 | MsG0280007909.01.T01:intron | 15.0% |
| !!! | ATATTTAGGTTTTTAAAAGT+AGG | - | Chr2:22789421-22789440 | None:intergenic | 15.0% |
| !!! | TCATTTTCTCAAAATTTTAT+CGG | + | Chr2:22789334-22789353 | MsG0280007909.01.T01:intron | 15.0% |
| !! | GACCATTACTTAATATATTT+AGG | - | Chr2:22789435-22789454 | None:intergenic | 20.0% |
| !!! | TTATTAAGATTTAGGGTTTA+GGG | + | Chr2:22789218-22789237 | MsG0280007909.01.T01:intron | 20.0% |
| !! | CTTATTAAGATTTAGGGTTT+AGG | + | Chr2:22789217-22789236 | MsG0280007909.01.T01:intron | 25.0% |
| !!! | AAAAGTATTGACATTGTTGA+AGG | + | Chr2:22789106-22789125 | MsG0280007909.01.T01:CDS | 25.0% |
| ATTGACATTGTTGAAGGAAA+TGG | + | Chr2:22789112-22789131 | MsG0280007909.01.T01:CDS | 30.0% | |
| CAAGTATGATTTACACAAAG+TGG | + | Chr2:22789504-22789523 | MsG0280007909.01.T01:CDS | 30.0% | |
| CACTTTGTGTAAATCATACT+TGG | - | Chr2:22789506-22789525 | None:intergenic | 30.0% | |
| TTGTCAAAAACTATGAAGCA+CGG | - | Chr2:22789389-22789408 | None:intergenic | 30.0% | |
| AACGAAAGTGAGTTTCTTGA+TGG | - | Chr2:22789149-22789168 | None:intergenic | 35.0% | |
| AAGAAACTCACTTTCGTTGA+AGG | + | Chr2:22789151-22789170 | MsG0280007909.01.T01:CDS | 35.0% | |
| AGTGTGAAATACTTCACCAA+AGG | + | Chr2:22789661-22789680 | MsG0280007909.01.T01:CDS | 35.0% | |
| ATGAATCTATGACATGCAGA+TGG | + | Chr2:22789475-22789494 | MsG0280007909.01.T01:intron | 35.0% | |
| TGATGACATCAACAACCTTT+GGG | - | Chr2:22789087-22789106 | None:intergenic | 35.0% | |
| TTTCACACTAAGCTTTGCAA+TGG | - | Chr2:22789650-22789669 | None:intergenic | 35.0% | |
| ! | TGATTTACACAAAGTGGACT+TGG | + | Chr2:22789510-22789529 | MsG0280007909.01.T01:CDS | 35.0% |
| ! | TTGATGACATCAACAACCTT+TGG | - | Chr2:22789088-22789107 | None:intergenic | 35.0% |
| ! | TTTGAAGCTAAATTGTCTGC+AGG | + | Chr2:22789610-22789629 | MsG0280007909.01.T01:CDS | 35.0% |
| ACTTGGTAGATGATGCTAAC+TGG | + | Chr2:22789527-22789546 | MsG0280007909.01.T01:CDS | 40.0% | |
| CTTGGTAGATGATGCTAACT+GGG | + | Chr2:22789528-22789547 | MsG0280007909.01.T01:CDS | 40.0% | |
| GAAATCTTCTCTACTGTGTC+TGG | - | Chr2:22789589-22789608 | None:intergenic | 40.0% | |
| GACATTGTTGAAGGAAATGG+TGG | + | Chr2:22789115-22789134 | MsG0280007909.01.T01:CDS | 40.0% | |
| GCTTACAACTACAGCATAGT+TGG | + | Chr2:22789550-22789569 | MsG0280007909.01.T01:CDS | 40.0% | |
| TACAACTACAGCATAGTTGG+AGG | + | Chr2:22789553-22789572 | MsG0280007909.01.T01:CDS | 40.0% | |
| TGATGTGTCTGTGTTGTGAT+TGG | + | Chr2:22789360-22789379 | MsG0280007909.01.T01:intron | 40.0% | |
| TTTGCAATGGATCCTCCATT+CGG | - | Chr2:22789637-22789656 | None:intergenic | 40.0% | |
| ! | AAAGCTTTGTGAAGTGTAGC+AGG | - | Chr2:22789040-22789059 | None:intergenic | 40.0% |
| ! | AGTGAGTTTCTTGATGGTTC+CGG | - | Chr2:22789143-22789162 | None:intergenic | 40.0% |
| !! | CATCAACAACCTTTGGGATA+AGG | - | Chr2:22789081-22789100 | None:intergenic | 40.0% |
| AAATTGTCTGCAGGTCCGAA+TGG | + | Chr2:22789619-22789638 | MsG0280007909.01.T01:CDS | 45.0% | |
| AAGTGTAGCAGGAGCTACAA+TGG | - | Chr2:22789029-22789048 | None:intergenic | 45.0% | |
| ACTAGGAGTAGCATCACCTT+TGG | - | Chr2:22789680-22789699 | None:intergenic | 45.0% | |
| AGCCAAACAGTAACCCTCAA+GGG | - | Chr2:22789752-22789771 | None:intergenic | 45.0% | |
| CTTGAAAAGACCTTCACCCT+TGG | - | Chr2:22789728-22789747 | None:intergenic | 45.0% | |
| TAGCCAAACAGTAACCCTCA+AGG | - | Chr2:22789753-22789772 | None:intergenic | 45.0% | |
| TGATGCTACTCCTAGTGAAG+AGG | + | Chr2:22789684-22789703 | MsG0280007909.01.T01:CDS | 45.0% | |
| TGCTGACAACCTTATCCCAA+AGG | + | Chr2:22789069-22789088 | MsG0280007909.01.T01:CDS | 45.0% | |
| ! | CAAGGGTGAAGGTCTTTTCA+AGG | + | Chr2:22789726-22789745 | MsG0280007909.01.T01:CDS | 45.0% |
| TGTAGCAGGAGCTACAATGG+AGG | - | Chr2:22789026-22789045 | None:intergenic | 50.0% | |
| ! | GCACCGACACTTCTGATTGA+CGG | + | Chr2:22789259-22789278 | MsG0280007909.01.T01:intron | 50.0% |
| ! | GCACCGTCAATCAGAAGTGT+CGG | - | Chr2:22789265-22789284 | None:intergenic | 50.0% |
| ! | TCGTGTCGATGTCGAATGTC+AGG | - | Chr2:22789290-22789309 | None:intergenic | 50.0% |
| !! | AGGTCTTTTCAAGGCCCTTG+AGG | + | Chr2:22789735-22789754 | MsG0280007909.01.T01:CDS | 50.0% |
| !! | CAGAAGTGTCGGTGCAACTT+AGG | - | Chr2:22789254-22789273 | None:intergenic | 50.0% |
| !! | GGTCTTTTCAAGGCCCTTGA+GGG | + | Chr2:22789736-22789755 | MsG0280007909.01.T01:CDS | 50.0% |
| AGCAGGAGCTACAATGGAGG+TGG | - | Chr2:22789023-22789042 | None:intergenic | 55.0% | |
| AGTGAAGAGGACCTCGAGAG+TGG | + | Chr2:22789697-22789716 | MsG0280007909.01.T01:CDS | 55.0% | |
| AGTGGCAAAGCCAAGGGTGA+AGG | + | Chr2:22789715-22789734 | MsG0280007909.01.T01:CDS | 55.0% | |
| CTCGAGAGTGGCAAAGCCAA+GGG | + | Chr2:22789709-22789728 | MsG0280007909.01.T01:CDS | 55.0% | |
| CTCTCGAGGTCCTCTTCACT+AGG | - | Chr2:22789697-22789716 | None:intergenic | 55.0% | |
| GGCCCTTGAGGGTTACTGTT+TGG | + | Chr2:22789747-22789766 | MsG0280007909.01.T01:CDS | 55.0% | |
| TTGTCTGCAGGTCCGAATGG+AGG | + | Chr2:22789622-22789641 | MsG0280007909.01.T01:CDS | 55.0% | |
| ! | GTTGAAGGAAATGGTGGCGC+CGG | + | Chr2:22789121-22789140 | MsG0280007909.01.T01:CDS | 55.0% |
| CCTCGAGAGTGGCAAAGCCA+AGG | + | Chr2:22789708-22789727 | MsG0280007909.01.T01:CDS | 60.0% | |
| !! | CCTTGGCTTTGCCACTCTCG+AGG | - | Chr2:22789711-22789730 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 22788992 | 22789789 | 22788992 | ID=MsG0280007909.01;Name=MsG0280007909.01 |
| Chr2 | mRNA | 22788992 | 22789789 | 22788992 | ID=MsG0280007909.01.T01;Parent=MsG0280007909.01;Name=MsG0280007909.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|1|1|2|0|158 |
| Chr2 | exon | 22788992 | 22789172 | 22788992 | ID=MsG0280007909.01.T01:exon:13870;Parent=MsG0280007909.01.T01 |
| Chr2 | exon | 22789494 | 22789789 | 22789494 | ID=MsG0280007909.01.T01:exon:13871;Parent=MsG0280007909.01.T01 |
| Chr2 | CDS | 22788992 | 22789172 | 22788992 | ID=MsG0280007909.01.T01:cds;Parent=MsG0280007909.01.T01 |
| Chr2 | CDS | 22789494 | 22789789 | 22789494 | ID=MsG0280007909.01.T01:cds;Parent=MsG0280007909.01.T01 |
| Gene Sequence |
| Protein sequence |
