Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036259.01.T01 | XP_027356688.1 | 62.176 | 193 | 63 | 3 | 1 | 190 | 85 | 270 | 1.36E-77 | 246 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036259.01.T01 | P21226 | 59.585 | 193 | 68 | 3 | 1 | 190 | 80 | 265 | 5.35E-76 | 234 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036259.01.T01 | A0A371FG58 | 62.694 | 193 | 62 | 3 | 1 | 190 | 98 | 283 | 5.31e-77 | 244 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000028.01 | MsG0780036259.01 | 0.801563 | 8.489332e-49 | 4.066946e-46 |
| MsG0180001029.01 | MsG0780036259.01 | 0.802767 | 4.798905e-49 | 2.370625e-46 |
| MsG0180003320.01 | MsG0780036259.01 | 0.805010 | 1.640738e-49 | 8.585396e-47 |
| MsG0180004059.01 | MsG0780036259.01 | 0.837289 | 5.676270e-57 | 7.339170e-54 |
| MsG0180004467.01 | MsG0780036259.01 | 0.803929 | 2.756392e-49 | 1.402868e-46 |
| MsG0180005059.01 | MsG0780036259.01 | 0.807742 | 4.356783e-50 | 2.445992e-47 |
| MsG0180005060.01 | MsG0780036259.01 | 0.834896 | 2.298886e-56 | 2.765713e-53 |
| MsG0180005082.01 | MsG0780036259.01 | 0.814232 | 1.711049e-51 | 1.141697e-48 |
| MsG0180005130.01 | MsG0780036259.01 | 0.817373 | 3.410232e-52 | 2.479171e-49 |
| MsG0180005143.01 | MsG0780036259.01 | 0.816283 | 5.990496e-52 | 4.227001e-49 |
| MsG0180005906.01 | MsG0780036259.01 | 0.811435 | 7.011407e-51 | 4.339885e-48 |
| MsG0180006253.01 | MsG0780036259.01 | 0.844003 | 9.890317e-59 | 1.575204e-55 |
| MsG0280006316.01 | MsG0780036259.01 | 0.813309 | 2.731711e-51 | 1.777678e-48 |
| MsG0280010623.01 | MsG0780036259.01 | 0.825491 | 4.552750e-54 | 4.155999e-51 |
| MsG0380013212.01 | MsG0780036259.01 | 0.803506 | 3.374807e-49 | 1.698941e-46 |
| MsG0380013213.01 | MsG0780036259.01 | 0.827857 | 1.241229e-54 | 1.213027e-51 |
| MsG0380013226.01 | MsG0780036259.01 | 0.805857 | 1.089998e-49 | 5.829471e-47 |
| MsG0380013239.01 | MsG0780036259.01 | 0.805640 | 1.210685e-49 | 6.438584e-47 |
| MsG0380013263.01 | MsG0780036259.01 | 0.807692 | 4.463436e-50 | 2.502479e-47 |
| MsG0380013843.01 | MsG0780036259.01 | 0.807196 | 5.688768e-50 | 3.148676e-47 |
| MsG0680031200.01 | MsG0780036259.01 | 0.806420 | 8.296063e-50 | 4.500820e-47 |
| MsG0680032026.01 | MsG0780036259.01 | 0.809149 | 2.182393e-50 | 1.271522e-47 |
| MsG0680034974.01 | MsG0780036259.01 | 0.813445 | 2.550014e-51 | 1.665685e-48 |
| MsG0480018559.01 | MsG0780036259.01 | 0.825856 | 3.730976e-54 | 3.441757e-51 |
| MsG0480018617.01 | MsG0780036259.01 | 0.815556 | 8.700206e-52 | 6.017901e-49 |
| MsG0480018622.01 | MsG0780036259.01 | 0.811672 | 6.227316e-51 | 3.878327e-48 |
| MsG0480018692.01 | MsG0780036259.01 | 0.801804 | 7.573033e-49 | 3.650295e-46 |
| MsG0480019322.01 | MsG0780036259.01 | 0.813030 | 3.145996e-51 | 2.031810e-48 |
| MsG0480019573.01 | MsG0780036259.01 | -0.804802 | 1.813285e-49 | 9.438178e-47 |
| MsG0480020285.01 | MsG0780036259.01 | 0.834580 | 2.761602e-56 | 3.291357e-53 |
| MsG0480020904.01 | MsG0780036259.01 | 0.809550 | 1.790670e-50 | 1.054499e-47 |
| MsG0480021091.01 | MsG0780036259.01 | 0.843595 | 1.271883e-58 | 1.999980e-55 |
| MsG0480021234.01 | MsG0780036259.01 | 0.842107 | 3.164160e-58 | 4.748171e-55 |
| MsG0480022298.01 | MsG0780036259.01 | 0.812120 | 4.974811e-51 | 3.135960e-48 |
| MsG0580024994.01 | MsG0780036259.01 | 0.824560 | 7.554411e-54 | 6.715268e-51 |
| MsG0580025007.01 | MsG0780036259.01 | 0.846281 | 2.396487e-59 | 4.103763e-56 |
| MsG0580025024.01 | MsG0780036259.01 | 0.850909 | 1.251877e-60 | 2.490272e-57 |
| MsG0580025252.01 | MsG0780036259.01 | -0.803728 | 3.035082e-49 | 1.536624e-46 |
| MsG0380016452.01 | MsG0780036259.01 | -0.800684 | 1.284066e-48 | 6.016282e-46 |
| MsG0380017168.01 | MsG0780036259.01 | 0.813377 | 2.640114e-51 | 1.721310e-48 |
| MsG0380017200.01 | MsG0780036259.01 | 0.810600 | 1.063237e-50 | 6.436846e-48 |
| MsG0480018362.01 | MsG0780036259.01 | 0.808997 | 2.351742e-50 | 1.364578e-47 |
| MsG0580026521.01 | MsG0780036259.01 | 0.827434 | 1.568255e-54 | 1.513860e-51 |
| MsG0280007513.01 | MsG0780036259.01 | 0.813207 | 2.877099e-51 | 1.866979e-48 |
| MsG0280007677.01 | MsG0780036259.01 | 0.828553 | 8.433874e-55 | 8.411127e-52 |
| MsG0280007689.01 | MsG0780036259.01 | 0.833301 | 5.768253e-56 | 6.615969e-53 |
| MsG0280007874.01 | MsG0780036259.01 | 0.803250 | 3.813088e-49 | 1.906902e-46 |
| MsG0280007909.01 | MsG0780036259.01 | 0.807093 | 5.982289e-50 | 3.302174e-47 |
| MsG0280010425.01 | MsG0780036259.01 | 0.823527 | 1.320374e-53 | 1.139740e-50 |
| MsG0780035930.01 | MsG0780036259.01 | 0.806006 | 1.014381e-49 | 5.445675e-47 |
| MsG0780036258.01 | MsG0780036259.01 | 0.930673 | 1.161976e-93 | 6.996842e-89 |
| MsG0780036259.01 | MsG0780036260.01 | 0.831205 | 1.904630e-55 | 2.052530e-52 |
| MsG0780036259.01 | MsG0780038657.01 | 0.810069 | 1.384333e-50 | 8.264342e-48 |
| MsG0780036259.01 | MsG0780039686.01 | 0.800316 | 1.525965e-48 | 7.083659e-46 |
| MsG0780036259.01 | MsG0780040670.01 | 0.805969 | 1.032612e-49 | 5.538247e-47 |
| MsG0780036259.01 | MsG0780040716.01 | 0.807464 | 4.991823e-50 | 2.782301e-47 |
| MsG0780036259.01 | MsG0780041385.01 | -0.815566 | 8.659126e-52 | 5.991052e-49 |
| MsG0780036259.01 | MsG0780041447.01 | 0.825991 | 3.465365e-54 | 3.208936e-51 |
| MsG0780036259.01 | MsG0780041823.01 | 0.821165 | 4.668528e-53 | 3.769360e-50 |
| MsG0780036259.01 | MsG0880042633.01 | 0.828493 | 8.718665e-55 | 8.680188e-52 |
| MsG0780036259.01 | MsG0880042728.01 | 0.830499 | 2.837884e-55 | 2.995056e-52 |
| MsG0780036259.01 | MsG0880044105.01 | -0.802378 | 5.772562e-49 | 2.823517e-46 |
| MsG0780036259.01 | MsG0880045854.01 | 0.801363 | 9.326677e-49 | 4.445783e-46 |
| MsG0780036259.01 | MsG0880046402.01 | 0.802111 | 6.550810e-49 | 3.182233e-46 |
| MsG0780036259.01 | MsG0880046408.01 | -0.806109 | 9.648021e-50 | 5.193031e-47 |
| MsG0780036259.01 | MsG0880046946.01 | 0.802708 | 4.936103e-49 | 2.434647e-46 |
| MsG0780036259.01 | MsG0880047312.01 | 0.826780 | 2.248774e-54 | 2.129532e-51 |
| MsG0780036259.01 | MsG0880047450.01 | 0.842918 | 1.928184e-58 | 2.967397e-55 |
| MsG0780036259.01 | MsG0880047460.01 | 0.801981 | 6.965102e-49 | 3.372217e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036259.01.T01 | MTR_3g118390 | 61.140 | 193 | 66 | 3 | 1 | 190 | 138 | 324 | 7.13e-79 | 241 |
| MsG0780036259.01.T01 | MTR_8g074350 | 51.813 | 193 | 83 | 4 | 1 | 190 | 92 | 277 | 1.53e-65 | 205 |
| MsG0780036259.01.T01 | MTR_8g074330 | 50.259 | 193 | 86 | 4 | 1 | 190 | 92 | 277 | 5.20e-62 | 196 |
| MsG0780036259.01.T01 | MTR_8g074335 | 47.150 | 193 | 79 | 3 | 1 | 190 | 94 | 266 | 4.18e-54 | 175 |
| MsG0780036259.01.T01 | MTR_7g110780 | 40.206 | 194 | 106 | 4 | 1 | 190 | 48 | 235 | 6.00e-48 | 158 |
| MsG0780036259.01.T01 | MTR_2g034680 | 35.025 | 197 | 116 | 6 | 1 | 190 | 68 | 259 | 2.19e-31 | 116 |
| MsG0780036259.01.T01 | MTR_7g021640 | 34.518 | 197 | 117 | 6 | 1 | 190 | 72 | 263 | 1.02e-30 | 115 |
| MsG0780036259.01.T01 | MTR_2g099465 | 39.007 | 141 | 73 | 3 | 7 | 145 | 69 | 198 | 3.63e-30 | 112 |
| MsG0780036259.01.T01 | MTR_2g099470 | 37.423 | 163 | 89 | 3 | 5 | 165 | 96 | 247 | 7.89e-30 | 112 |
| MsG0780036259.01.T01 | MTR_1g105535 | 34.010 | 197 | 118 | 6 | 1 | 190 | 75 | 266 | 1.74e-29 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0780036259.01.T01 | AT3G12500 | 56.995 | 193 | 74 | 3 | 1 | 190 | 98 | 284 | 3.70e-74 | 227 |
| MsG0780036259.01.T01 | AT4G01700 | 44.330 | 194 | 98 | 4 | 1 | 190 | 50 | 237 | 1.34e-53 | 173 |
| MsG0780036259.01.T01 | AT1G02360 | 41.753 | 194 | 103 | 4 | 1 | 190 | 42 | 229 | 2.24e-49 | 162 |
| MsG0780036259.01.T01 | AT1G02360 | 42.408 | 191 | 100 | 4 | 4 | 190 | 97 | 281 | 5.66e-49 | 162 |
| MsG0780036259.01.T01 | AT1G02360 | 41.753 | 194 | 103 | 4 | 1 | 190 | 88 | 275 | 6.11e-49 | 162 |
| MsG0780036259.01.T01 | AT3G54420 | 40.881 | 159 | 81 | 3 | 8 | 164 | 90 | 237 | 3.11e-33 | 120 |
| MsG0780036259.01.T01 | AT2G43590 | 36.364 | 165 | 88 | 4 | 2 | 164 | 80 | 229 | 4.00e-33 | 120 |
| MsG0780036259.01.T01 | AT1G05850 | 35.533 | 197 | 115 | 6 | 1 | 190 | 72 | 263 | 1.17e-30 | 115 |
| MsG0780036259.01.T01 | AT1G05850 | 35.533 | 197 | 115 | 6 | 1 | 190 | 72 | 263 | 1.17e-30 | 115 |
| MsG0780036259.01.T01 | AT3G16920 | 33.503 | 197 | 119 | 6 | 1 | 190 | 80 | 271 | 2.87e-30 | 114 |
| MsG0780036259.01.T01 | AT3G16920 | 33.503 | 197 | 119 | 6 | 1 | 190 | 104 | 295 | 3.69e-30 | 114 |
| MsG0780036259.01.T01 | AT2G43580 | 34.254 | 181 | 96 | 6 | 2 | 180 | 81 | 240 | 9.53e-30 | 111 |
| MsG0780036259.01.T01 | AT2G43610 | 36.842 | 152 | 80 | 3 | 2 | 153 | 100 | 235 | 1.43e-27 | 105 |
| MsG0780036259.01.T01 | AT2G43620 | 38.028 | 142 | 72 | 3 | 2 | 143 | 102 | 227 | 3.03e-27 | 105 |
| MsG0780036259.01.T01 | AT1G56680 | 41.611 | 149 | 70 | 5 | 2 | 149 | 96 | 228 | 1.07e-26 | 103 |
| MsG0780036259.01.T01 | AT1G56680 | 42.657 | 143 | 65 | 5 | 2 | 143 | 119 | 245 | 1.68e-26 | 103 |
| MsG0780036259.01.T01 | AT2G43600 | 37.584 | 149 | 75 | 4 | 2 | 149 | 92 | 223 | 3.28e-24 | 97.1 |
| MsG0780036259.01.T01 | AT2G43600 | 37.584 | 149 | 75 | 4 | 2 | 149 | 113 | 244 | 5.05e-24 | 97.1 |
| MsG0780036259.01.T01 | AT2G43570 | 37.500 | 144 | 77 | 4 | 1 | 143 | 89 | 220 | 7.46e-24 | 96.3 |
| MsG0780036259.01.T01 | AT3G47540 | 27.044 | 159 | 87 | 6 | 8 | 164 | 48 | 179 | 4.06e-11 | 60.5 |
Find 75 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTCCTTCGTTGCCGAACC+TGG | 0.245191 | 7:-5463823 | None:intergenic |
| CAGAGGAGGTTTCCAGGATA+TGG | 0.257878 | 7:+5464260 | MsG0780036259.01.T01:CDS |
| TCAAGAGTGCTATATGGTTT+TGG | 0.289569 | 7:+5464144 | MsG0780036259.01.T01:CDS |
| TCTAAATTTCCATGTGTTCC+TGG | 0.293914 | 7:+5463993 | MsG0780036259.01.T01:CDS |
| ATCAAAGGGAATAAAATATA+TGG | 0.311345 | 7:+5464047 | MsG0780036259.01.T01:CDS |
| GGAGAATGTGGAAGTATAAT+AGG | 0.323922 | 7:+5464068 | MsG0780036259.01.T01:CDS |
| AAAGAAATATCCCCGGTCAT+AGG | 0.328899 | 7:-5463925 | None:intergenic |
| GCCGAACCTGGGGAATGTTT+TGG | 0.339992 | 7:-5463811 | None:intergenic |
| GGGTCTGTGGCAATGAGATC+TGG | 0.352495 | 7:-5464110 | None:intergenic |
| CGTGGCAAGATGGCTTTACT+TGG | 0.369053 | 7:-5464193 | None:intergenic |
| GCACTCTTGAAGGATATAAC+AGG | 0.377927 | 7:-5464131 | None:intergenic |
| TTTAGTCAGAGGAGGTTTCC+AGG | 0.389026 | 7:+5464254 | MsG0780036259.01.T01:CDS |
| AAGAAATATCCCCGGTCATA+GGG | 0.408514 | 7:-5463924 | None:intergenic |
| GGAATGCGCGACCGGGTGGC+TGG | 0.413441 | 7:+5464314 | MsG0780036259.01.T01:CDS |
| TATCCTTCAAGAGTGCTATA+TGG | 0.418078 | 7:+5464138 | MsG0780036259.01.T01:CDS |
| TCTTGCCACGACGCTATAAC+CGG | 0.430220 | 7:+5464206 | MsG0780036259.01.T01:CDS |
| AAACCATATAGCACTCTTGA+AGG | 0.434167 | 7:-5464141 | None:intergenic |
| GCTGGGAAGGAATGCGCGAC+CGG | 0.453510 | 7:+5464306 | MsG0780036259.01.T01:CDS |
| TCTCCTTCGTTGCCGAACCT+GGG | 0.454761 | 7:-5463822 | None:intergenic |
| GCCAAAACATTCCCCAGGTT+CGG | 0.455985 | 7:+5463810 | MsG0780036259.01.T01:CDS |
| CTAAATTTCCATGTGTTCCT+GGG | 0.462737 | 7:+5463994 | MsG0780036259.01.T01:CDS |
| ACACATGGAAATTTAGAGCT+TGG | 0.463623 | 7:-5463987 | None:intergenic |
| CACCTCTTAGCCCCTATGAC+CGG | 0.464103 | 7:+5463913 | MsG0780036259.01.T01:CDS |
| TGACTAAAATCAAAATCAGT+AGG | 0.468194 | 7:-5464239 | None:intergenic |
| TTCGCAGTAGTCTTCTTCGA+TGG | 0.468797 | 7:-5463964 | None:intergenic |
| CTCTCGTAGTATTGCTTCCC+AGG | 0.474080 | 7:-5464011 | None:intergenic |
| ACCTCTTAGCCCCTATGACC+GGG | 0.474353 | 7:+5463914 | MsG0780036259.01.T01:CDS |
| CAAGATGGCTTTACTTGGAA+AGG | 0.479312 | 7:-5464188 | None:intergenic |
| ATGACCACATATGTCATAGA+AGG | 0.491232 | 7:+5464284 | MsG0780036259.01.T01:CDS |
| TCGCAGTAGTCTTCTTCGAT+GGG | 0.491948 | 7:-5463963 | None:intergenic |
| CTGGGAAGGAATGCGCGACC+GGG | 0.494873 | 7:+5464307 | MsG0780036259.01.T01:CDS |
| CCCCGGTCATAGGGGCTAAG+AGG | 0.498386 | 7:-5463915 | None:intergenic |
| CCACATATGTCATAGAAGGC+TGG | 0.498873 | 7:+5464288 | MsG0780036259.01.T01:CDS |
| AGAGGAGGTTTCCAGGATAT+GGG | 0.498919 | 7:+5464261 | MsG0780036259.01.T01:CDS |
| AAGATGGCTTTACTTGGAAA+GGG | 0.503243 | 7:-5464187 | None:intergenic |
| TATGTGGTCATCCCATATCC+TGG | 0.504283 | 7:-5464272 | None:intergenic |
| ATCAGTAGGGATCCATCGTC+CGG | 0.511652 | 7:-5464225 | None:intergenic |
| TTATTCCCTTTGATCTGAAG+CGG | 0.516176 | 7:-5464038 | None:intergenic |
| CACATATGTCATAGAAGGCT+GGG | 0.516692 | 7:+5464289 | MsG0780036259.01.T01:CDS |
| AATAAAATATATGGAGAATG+TGG | 0.517331 | 7:+5464056 | MsG0780036259.01.T01:CDS |
| AGGGGTCCGCTTCAGATCAA+AGG | 0.525246 | 7:+5464032 | MsG0780036259.01.T01:CDS |
| GGCTTTACTTGGAAAGGGAA+AGG | 0.540330 | 7:-5464182 | None:intergenic |
| GGTCTGTGGCAATGAGATCT+GGG | 0.547304 | 7:-5464109 | None:intergenic |
| CCAGCCTTCTATGACATATG+TGG | 0.551787 | 7:-5464288 | None:intergenic |
| GGATGTAAACGAGATCGATC+GGG | 0.553292 | 7:+5464335 | MsG0780036259.01.T01:CDS |
| ACTTGGAAAGGGAAAGGTGG+AGG | 0.555116 | 7:-5464176 | None:intergenic |
| GGGGTCCGCTTCAGATCAAA+GGG | 0.557701 | 7:+5464033 | MsG0780036259.01.T01:CDS |
| AAGCTGCCAAAACATTCCCC+AGG | 0.566465 | 7:+5463805 | MsG0780036259.01.T01:CDS |
| GACTAAAATCAAAATCAGTA+GGG | 0.566815 | 7:-5464238 | None:intergenic |
| TGGATGTAAACGAGATCGAT+CGG | 0.570882 | 7:+5464334 | MsG0780036259.01.T01:CDS |
| CTCGTTTACATCCAGCCACC+CGG | 0.571779 | 7:-5464325 | None:intergenic |
| GATGATGAGTGTGAGGGGAA+AGG | 0.572756 | 7:+5463756 | MsG0780036259.01.T01:CDS |
| AGCTCTCACAAAACAAGAGG+TGG | 0.574719 | 7:+5463888 | MsG0780036259.01.T01:CDS |
| GGAAGGAATGCGCGACCGGG+TGG | 0.575460 | 7:+5464310 | MsG0780036259.01.T01:CDS |
| CTCCTTCGTTGCCGAACCTG+GGG | 0.576604 | 7:-5463821 | None:intergenic |
| TATTGCTTCCCAGGAACACA+TGG | 0.580157 | 7:-5464002 | None:intergenic |
| TTCCCCAGGTTCGGCAACGA+AGG | 0.585501 | 7:+5463819 | MsG0780036259.01.T01:CDS |
| GTTATAGCGTCGTGGCAAGA+TGG | 0.586870 | 7:-5464203 | None:intergenic |
| CACTCTTGAAGGATATAACA+GGG | 0.603547 | 7:-5464130 | None:intergenic |
| CGCGACGATGATGAGTGTGA+GGG | 0.608511 | 7:+5463750 | MsG0780036259.01.T01:CDS |
| TATGTCATAGAAGGCTGGGA+AGG | 0.610194 | 7:+5464293 | MsG0780036259.01.T01:CDS |
| CATTCACAAAGAAATATCCC+CGG | 0.613688 | 7:-5463932 | None:intergenic |
| AGCTTCAACAAATGCTTCGT+AGG | 0.613992 | 7:-5463787 | None:intergenic |
| TCGCGACGATGATGAGTGTG+AGG | 0.615130 | 7:+5463749 | MsG0780036259.01.T01:CDS |
| AGAAATATCCCCGGTCATAG+GGG | 0.616770 | 7:-5463923 | None:intergenic |
| GAAGGATATAACAGGGTCTG+TGG | 0.619235 | 7:-5464123 | None:intergenic |
| CCTCTTAGCCCCTATGACCG+GGG | 0.619811 | 7:+5463915 | MsG0780036259.01.T01:CDS |
| CTGGGAAGCAATACTACGAG+AGG | 0.622203 | 7:+5464012 | MsG0780036259.01.T01:CDS |
| CAAAGCTCTCACAAAACAAG+AGG | 0.630156 | 7:+5463885 | MsG0780036259.01.T01:CDS |
| TGGGAAGCAATACTACGAGA+GGG | 0.631128 | 7:+5464013 | MsG0780036259.01.T01:CDS |
| TTTACTTGGAAAGGGAAAGG+TGG | 0.645661 | 7:-5464179 | None:intergenic |
| ACGACGCTATAACCGGACGA+TGG | 0.658263 | 7:+5464213 | MsG0780036259.01.T01:CDS |
| GCGACGATGATGAGTGTGAG+GGG | 0.672599 | 7:+5463751 | MsG0780036259.01.T01:CDS |
| ATCGTCCGGTTATAGCGTCG+TGG | 0.679868 | 7:-5464211 | None:intergenic |
| GGGAAGCAATACTACGAGAG+GGG | 0.685899 | 7:+5464014 | MsG0780036259.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATAAAATATATGGAGAATG+TGG | + | Chr7:5464056-5464075 | MsG0780036259.01.T01:CDS | 20.0% |
| !! | ATCAAAGGGAATAAAATATA+TGG | + | Chr7:5464047-5464066 | MsG0780036259.01.T01:CDS | 20.0% |
| ! | GACTAAAATCAAAATCAGTA+GGG | - | Chr7:5464241-5464260 | None:intergenic | 25.0% |
| ! | TGACTAAAATCAAAATCAGT+AGG | - | Chr7:5464242-5464261 | None:intergenic | 25.0% |
| !!! | ATTTTGATTTTAGTCAGAGG+AGG | + | Chr7:5464246-5464265 | MsG0780036259.01.T01:CDS | 30.0% |
| !!! | CTGATTTTGATTTTAGTCAG+AGG | + | Chr7:5464243-5464262 | MsG0780036259.01.T01:CDS | 30.0% |
| AAACCATATAGCACTCTTGA+AGG | - | Chr7:5464144-5464163 | None:intergenic | 35.0% | |
| ATGACCACATATGTCATAGA+AGG | + | Chr7:5464284-5464303 | MsG0780036259.01.T01:CDS | 35.0% | |
| CACTCTTGAAGGATATAACA+GGG | - | Chr7:5464133-5464152 | None:intergenic | 35.0% | |
| CATTCACAAAGAAATATCCC+CGG | - | Chr7:5463935-5463954 | None:intergenic | 35.0% | |
| CTAAATTTCCATGTGTTCCT+GGG | + | Chr7:5463994-5464013 | MsG0780036259.01.T01:CDS | 35.0% | |
| TCTAAATTTCCATGTGTTCC+TGG | + | Chr7:5463993-5464012 | MsG0780036259.01.T01:CDS | 35.0% | |
| TTATTCCCTTTGATCTGAAG+CGG | - | Chr7:5464041-5464060 | None:intergenic | 35.0% | |
| ! | AAGATGGCTTTACTTGGAAA+GGG | - | Chr7:5464190-5464209 | None:intergenic | 35.0% |
| ! | ACACATGGAAATTTAGAGCT+TGG | - | Chr7:5463990-5464009 | None:intergenic | 35.0% |
| ! | GGAGAATGTGGAAGTATAAT+AGG | + | Chr7:5464068-5464087 | MsG0780036259.01.T01:CDS | 35.0% |
| !! | TATCCTTCAAGAGTGCTATA+TGG | + | Chr7:5464138-5464157 | MsG0780036259.01.T01:CDS | 35.0% |
| !!! | AGAGACATTGCTGCTTTTTT+TGG | + | Chr7:5463861-5463880 | MsG0780036259.01.T01:CDS | 35.0% |
| !!! | TCAAGAGTGCTATATGGTTT+TGG | + | Chr7:5464144-5464163 | MsG0780036259.01.T01:CDS | 35.0% |
| AAAGAAATATCCCCGGTCAT+AGG | - | Chr7:5463928-5463947 | None:intergenic | 40.0% | |
| AAGAAATATCCCCGGTCATA+GGG | - | Chr7:5463927-5463946 | None:intergenic | 40.0% | |
| AGCTTCAACAAATGCTTCGT+AGG | - | Chr7:5463790-5463809 | None:intergenic | 40.0% | |
| CAAAGCTCTCACAAAACAAG+AGG | + | Chr7:5463885-5463904 | MsG0780036259.01.T01:CDS | 40.0% | |
| CACATATGTCATAGAAGGCT+GGG | + | Chr7:5464289-5464308 | MsG0780036259.01.T01:CDS | 40.0% | |
| GCACTCTTGAAGGATATAAC+AGG | - | Chr7:5464134-5464153 | None:intergenic | 40.0% | |
| TGGATGTAAACGAGATCGAT+CGG | + | Chr7:5464334-5464353 | MsG0780036259.01.T01:CDS | 40.0% | |
| TTTACTTGGAAAGGGAAAGG+TGG | - | Chr7:5464182-5464201 | None:intergenic | 40.0% | |
| ! | CAAGATGGCTTTACTTGGAA+AGG | - | Chr7:5464191-5464210 | None:intergenic | 40.0% |
| AGAAATATCCCCGGTCATAG+GGG | - | Chr7:5463926-5463945 | None:intergenic | 45.0% | |
| AGAGGAGGTTTCCAGGATAT+GGG | + | Chr7:5464261-5464280 | MsG0780036259.01.T01:CDS | 45.0% | |
| AGCTCTCACAAAACAAGAGG+TGG | + | Chr7:5463888-5463907 | MsG0780036259.01.T01:CDS | 45.0% | |
| CCACATATGTCATAGAAGGC+TGG | + | Chr7:5464288-5464307 | MsG0780036259.01.T01:CDS | 45.0% | |
| CCAGCCTTCTATGACATATG+TGG | - | Chr7:5464291-5464310 | None:intergenic | 45.0% | |
| GAAGGATATAACAGGGTCTG+TGG | - | Chr7:5464126-5464145 | None:intergenic | 45.0% | |
| GGATGTAAACGAGATCGATC+GGG | + | Chr7:5464335-5464354 | MsG0780036259.01.T01:CDS | 45.0% | |
| GGCTTTACTTGGAAAGGGAA+AGG | - | Chr7:5464185-5464204 | None:intergenic | 45.0% | |
| TATGTCATAGAAGGCTGGGA+AGG | + | Chr7:5464293-5464312 | MsG0780036259.01.T01:CDS | 45.0% | |
| TATGTGGTCATCCCATATCC+TGG | - | Chr7:5464275-5464294 | None:intergenic | 45.0% | |
| TATTGCTTCCCAGGAACACA+TGG | - | Chr7:5464005-5464024 | None:intergenic | 45.0% | |
| TCGCAGTAGTCTTCTTCGAT+GGG | - | Chr7:5463966-5463985 | None:intergenic | 45.0% | |
| TGGGAAGCAATACTACGAGA+GGG | + | Chr7:5464013-5464032 | MsG0780036259.01.T01:CDS | 45.0% | |
| TTCGCAGTAGTCTTCTTCGA+TGG | - | Chr7:5463967-5463986 | None:intergenic | 45.0% | |
| TTTAGTCAGAGGAGGTTTCC+AGG | + | Chr7:5464254-5464273 | MsG0780036259.01.T01:CDS | 45.0% | |
| AAGCTGCCAAAACATTCCCC+AGG | + | Chr7:5463805-5463824 | MsG0780036259.01.T01:CDS | 50.0% | |
| ACTTGGAAAGGGAAAGGTGG+AGG | - | Chr7:5464179-5464198 | None:intergenic | 50.0% | |
| ATCAGTAGGGATCCATCGTC+CGG | - | Chr7:5464228-5464247 | None:intergenic | 50.0% | |
| CAGAGGAGGTTTCCAGGATA+TGG | + | Chr7:5464260-5464279 | MsG0780036259.01.T01:CDS | 50.0% | |
| CTCTCGTAGTATTGCTTCCC+AGG | - | Chr7:5464014-5464033 | None:intergenic | 50.0% | |
| CTGGGAAGCAATACTACGAG+AGG | + | Chr7:5464012-5464031 | MsG0780036259.01.T01:CDS | 50.0% | |
| GATGATGAGTGTGAGGGGAA+AGG | + | Chr7:5463756-5463775 | MsG0780036259.01.T01:CDS | 50.0% | |
| GCCAAAACATTCCCCAGGTT+CGG | + | Chr7:5463810-5463829 | MsG0780036259.01.T01:CDS | 50.0% | |
| GGGAAGCAATACTACGAGAG+GGG | + | Chr7:5464014-5464033 | MsG0780036259.01.T01:CDS | 50.0% | |
| GGTCTGTGGCAATGAGATCT+GGG | - | Chr7:5464112-5464131 | None:intergenic | 50.0% | |
| GTTATAGCGTCGTGGCAAGA+TGG | - | Chr7:5464206-5464225 | None:intergenic | 50.0% | |
| TCTTGCCACGACGCTATAAC+CGG | + | Chr7:5464206-5464225 | MsG0780036259.01.T01:CDS | 50.0% | |
| ! | CGTGGCAAGATGGCTTTACT+TGG | - | Chr7:5464196-5464215 | None:intergenic | 50.0% |
| ACCTCTTAGCCCCTATGACC+GGG | + | Chr7:5463914-5463933 | MsG0780036259.01.T01:CDS | 55.0% | |
| ACGACGCTATAACCGGACGA+TGG | + | Chr7:5464213-5464232 | MsG0780036259.01.T01:CDS | 55.0% | |
| AGGGGTCCGCTTCAGATCAA+AGG | + | Chr7:5464032-5464051 | MsG0780036259.01.T01:CDS | 55.0% | |
| ATCGTCCGGTTATAGCGTCG+TGG | - | Chr7:5464214-5464233 | None:intergenic | 55.0% | |
| CACCTCTTAGCCCCTATGAC+CGG | + | Chr7:5463913-5463932 | MsG0780036259.01.T01:CDS | 55.0% | |
| CGCGACGATGATGAGTGTGA+GGG | + | Chr7:5463750-5463769 | MsG0780036259.01.T01:CDS | 55.0% | |
| CTCGTTTACATCCAGCCACC+CGG | - | Chr7:5464328-5464347 | None:intergenic | 55.0% | |
| GCGACGATGATGAGTGTGAG+GGG | + | Chr7:5463751-5463770 | MsG0780036259.01.T01:CDS | 55.0% | |
| GGGGTCCGCTTCAGATCAAA+GGG | + | Chr7:5464033-5464052 | MsG0780036259.01.T01:CDS | 55.0% | |
| GGGTCTGTGGCAATGAGATC+TGG | - | Chr7:5464113-5464132 | None:intergenic | 55.0% | |
| TCGCGACGATGATGAGTGTG+AGG | + | Chr7:5463749-5463768 | MsG0780036259.01.T01:CDS | 55.0% | |
| TCTCCTTCGTTGCCGAACCT+GGG | - | Chr7:5463825-5463844 | None:intergenic | 55.0% | |
| ! | GCCGAACCTGGGGAATGTTT+TGG | - | Chr7:5463814-5463833 | None:intergenic | 55.0% |
| CCTCTTAGCCCCTATGACCG+GGG | + | Chr7:5463915-5463934 | MsG0780036259.01.T01:CDS | 60.0% | |
| CTCCTTCGTTGCCGAACCTG+GGG | - | Chr7:5463824-5463843 | None:intergenic | 60.0% | |
| GTCTCCTTCGTTGCCGAACC+TGG | - | Chr7:5463826-5463845 | None:intergenic | 60.0% | |
| TTCCCCAGGTTCGGCAACGA+AGG | + | Chr7:5463819-5463838 | MsG0780036259.01.T01:CDS | 60.0% | |
| CCCCGGTCATAGGGGCTAAG+AGG | - | Chr7:5463918-5463937 | None:intergenic | 65.0% | |
| CTGGGAAGGAATGCGCGACC+GGG | + | Chr7:5464307-5464326 | MsG0780036259.01.T01:CDS | 65.0% | |
| GCTGGGAAGGAATGCGCGAC+CGG | + | Chr7:5464306-5464325 | MsG0780036259.01.T01:CDS | 65.0% | |
| GGAAGGAATGCGCGACCGGG+TGG | + | Chr7:5464310-5464329 | MsG0780036259.01.T01:CDS | 70.0% | |
| GGAATGCGCGACCGGGTGGC+TGG | + | Chr7:5464314-5464333 | MsG0780036259.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr7 | gene | 5463738 | 5464370 | 5463738 | ID=MsG0780036259.01;Name=MsG0780036259.01 |
| Chr7 | mRNA | 5463738 | 5464370 | 5463738 | ID=MsG0780036259.01.T01;Parent=MsG0780036259.01;Name=MsG0780036259.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|210 |
| Chr7 | exon | 5463738 | 5464370 | 5463738 | ID=MsG0780036259.01.T01:exon:2935;Parent=MsG0780036259.01.T01 |
| Chr7 | CDS | 5463738 | 5464370 | 5463738 | ID=MsG0780036259.01.T01:cds;Parent=MsG0780036259.01.T01 |
| Gene Sequence |
| Protein sequence |
