AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380014142.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014142.01.T01 MTR_7g094120 87.421 159 20 0 1 159 1 159 5.06e-102 290
MsG0380014142.01.T01 MTR_7g021400 82.390 159 25 2 1 159 1 156 9.10e-85 246
MsG0380014142.01.T01 MTR_7g094150 79.874 159 29 2 1 159 1 156 8.51e-82 238
MsG0380014142.01.T01 MTR_7g094280 73.125 160 40 2 1 159 1 158 8.80e-81 236
MsG0380014142.01.T01 MTR_5g022710 77.358 159 32 2 1 159 1 155 6.81e-79 231
MsG0380014142.01.T01 MTR_3g110048 72.671 161 39 3 1 159 1 158 1.39e-78 230
MsG0380014142.01.T01 MTR_7g094170 77.987 159 32 2 1 159 1 156 1.42e-78 230
MsG0380014142.01.T01 MTR_5g022730 76.101 159 34 2 1 159 1 155 1.65e-77 227
MsG0380014142.01.T01 MTR_7g021380 77.358 159 33 2 1 159 1 156 2.32e-76 224
MsG0380014142.01.T01 MTR_4g032450 68.790 157 46 2 4 159 21 175 1.83e-75 223
MsG0380014142.01.T01 MTR_4g032400 69.811 159 45 2 2 159 7 163 1.40e-73 218
MsG0380014142.01.T01 MTR_7g094310 68.125 160 38 3 1 159 1 148 6.73e-72 213
MsG0380014142.01.T01 MTR_8g056060 66.875 160 49 3 1 159 1 157 8.05e-69 206
MsG0380014142.01.T01 MTR_4g032510 63.975 161 48 3 1 159 1 153 4.91e-68 203
MsG0380014142.01.T01 MTR_4g032500 61.875 160 52 2 1 159 1 152 2.74e-67 201
MsG0380014142.01.T01 MTR_8g468480 65.000 160 51 3 1 159 1 156 2.16e-65 197
MsG0380014142.01.T01 MTR_5g085030 61.635 159 56 3 1 158 1 155 1.96e-63 192
MsG0380014142.01.T01 MTR_4g032460 65.000 160 45 3 1 159 1 150 4.10e-63 191
MsG0380014142.01.T01 MTR_4g033455 59.236 157 61 2 3 159 7 160 5.21e-62 188
MsG0380014142.01.T01 MTR_4g017860 52.062 194 52 3 7 159 27 220 2.23e-57 179
MsG0380014142.01.T01 MTR_4g033460 59.441 143 55 2 3 145 7 146 9.66e-55 173
MsG0380014142.01.T01 MTR_4g033460 72.973 74 18 1 42 115 161 232 7.19e-29 106
MsG0380014142.01.T01 MTR_5g082340 58.125 160 51 3 1 159 1 145 1.22e-52 164
MsG0380014142.01.T01 MTR_1g099870 75.962 104 21 2 58 159 2 103 3.06e-51 159
MsG0380014142.01.T01 MTR_4g021390 55.063 158 66 2 2 159 3 155 2.24e-50 159
MsG0380014142.01.T01 MTR_8g089730 65.600 125 24 1 35 159 1 106 1.91e-48 152
MsG0380014142.01.T01 MTR_7g094320 50.943 159 36 1 1 159 1 117 1.38e-45 145
MsG0380014142.01.T01 MTR_4g032420 55.814 129 40 3 32 159 38 150 1.75e-44 144
MsG0380014142.01.T01 MTR_7g094410 53.846 156 33 4 1 117 1 156 1.48e-41 137
MsG0380014142.01.T01 MTR_4g032470 49.315 146 28 2 15 159 34 134 8.18e-40 131
MsG0380014142.01.T01 MTR_4g131980 55.797 138 48 6 1 136 1 127 1.72e-37 125
MsG0380014142.01.T01 MTR_7g094360 44.509 173 44 3 1 122 1 172 9.86e-37 125
MsG0380014142.01.T01 MTR_4g127240 63.810 105 30 4 41 143 26 124 4.36e-36 121
MsG0380014142.01.T01 MTR_7g006370 50.694 144 60 3 2 145 3 135 5.55e-36 121
MsG0380014142.01.T01 MTR_7g094350 43.258 178 45 4 1 125 1 175 5.26e-32 113
MsG0380014142.01.T01 MTR_8g056090 46.541 159 49 8 1 157 1 125 2.62e-28 102
MsG0380014142.01.T01 MTR_4g119422 42.282 149 77 2 3 149 227 368 6.42e-28 106
MsG0380014142.01.T01 MTR_1g112590 35.897 156 80 4 11 149 3 155 1.54e-26 98.2
MsG0380014142.01.T01 MTR_4g032520 46.281 121 38 2 31 150 2 96 1.41e-25 95.1
MsG0380014142.01.T01 MTR_7g071200 62.319 69 25 1 92 159 4 72 7.17e-25 91.7
MsG0380014142.01.T01 MTR_2g066010 37.342 158 87 5 3 158 14 161 8.35e-24 92.4
MsG0380014142.01.T01 MTR_2g065720 37.342 158 87 5 3 158 31 178 4.27e-23 90.9
MsG0380014142.01.T01 MTR_2g018980 32.895 152 87 3 9 159 3 140 1.53e-21 85.1
MsG0380014142.01.T01 MTR_4g032540 60.317 63 24 1 93 154 70 132 1.65e-21 84.7
MsG0380014142.01.T01 MTR_5g096950 36.250 160 82 5 1 158 1 142 7.11e-20 80.9
MsG0380014142.01.T01 MTR_8g007195 33.803 142 80 4 11 146 13 146 1.02e-17 78.6
MsG0380014142.01.T01 MTR_6g053620 32.903 155 90 4 1 149 9 155 2.57e-17 77.8
MsG0380014142.01.T01 MTR_6g053620 32.903 155 90 4 1 149 9 155 3.91e-17 77.4
MsG0380014142.01.T01 MTR_2g100830 31.126 151 88 4 6 149 8 149 4.33e-17 76.6
MsG0380014142.01.T01 MTR_5g078680 31.410 156 67 4 11 149 3 135 9.84e-17 75.1
MsG0380014142.01.T01 MTR_2g100825 30.667 150 91 3 5 149 7 148 1.92e-16 75.1
MsG0380014142.01.T01 MTR_1g042510 31.013 158 69 4 9 149 1 135 3.39e-16 73.9
MsG0380014142.01.T01 MTR_1g112570 35.461 141 64 6 9 131 1 132 3.42e-16 73.9
MsG0380014142.01.T01 MTR_8g007185 29.252 147 89 4 11 150 13 151 2.84e-15 71.6
MsG0380014142.01.T01 MTR_8g007205 29.252 147 89 4 11 150 13 151 4.99e-15 70.9
MsG0380014142.01.T01 MTR_8g030670 33.333 102 61 1 48 149 62 156 1.17e-14 70.5
MsG0380014142.01.T01 MTR_7g077810 30.464 151 89 4 6 149 15 156 1.94e-14 69.7
MsG0380014142.01.T01 MTR_7g036780 29.167 144 88 3 1 138 1 136 1.09e-12 63.9
MsG0380014142.01.T01 MTR_3g061700 32.990 97 56 2 54 149 1 89 5.02e-11 58.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380014142.01.T01 AT1G20140 70.000 160 43 1 5 159 4 163 3.36e-78 229
MsG0380014142.01.T01 AT2G25700 67.901 162 43 2 5 159 4 163 1.84e-74 220
MsG0380014142.01.T01 AT1G75950 71.069 159 42 1 5 159 2 160 2.24e-73 217
MsG0380014142.01.T01 AT5G42190 65.294 170 45 1 4 159 2 171 1.24e-70 211
MsG0380014142.01.T01 AT4G34210 64.516 155 51 1 5 159 2 152 3.68e-70 209
MsG0380014142.01.T01 AT4G34470 62.581 155 54 1 5 159 2 152 1.93e-67 202
MsG0380014142.01.T01 AT3G60010 61.290 155 55 2 7 159 3 154 7.56e-63 190
MsG0380014142.01.T01 AT2G03170 59.211 152 53 4 9 159 6 149 5.11e-58 178
MsG0380014142.01.T01 AT2G03190 54.938 162 62 2 9 159 6 167 2.91e-57 177
MsG0380014142.01.T01 AT3G21860 57.692 156 61 2 4 159 2 152 3.16e-57 176
MsG0380014142.01.T01 AT3G21850 56.688 157 62 3 4 159 2 153 7.10e-57 175
MsG0380014142.01.T01 AT2G03160 48.148 189 64 4 5 159 2 190 8.86e-52 164
MsG0380014142.01.T01 AT3G25650 56.173 162 60 4 9 159 6 167 1.24e-51 162
MsG0380014142.01.T01 AT3G60020 52.229 157 61 4 9 159 5 153 6.03e-51 160
MsG0380014142.01.T01 AT2G20160 53.548 155 64 2 5 158 2 149 9.18e-51 159
MsG0380014142.01.T01 AT1G10230 54.305 151 68 1 9 158 6 156 4.72e-49 155
MsG0380014142.01.T01 AT3G21830 51.923 156 70 2 4 159 2 152 8.23e-49 155
MsG0380014142.01.T01 AT3G21840 50.413 121 55 2 4 124 2 117 6.85e-30 105
MsG0380014142.01.T01 AT3G53060 59.302 86 26 3 32 116 3 80 3.94e-25 92.8
MsG0380014142.01.T01 AT2G45950 31.788 151 91 4 4 149 13 156 1.28e-16 75.9
MsG0380014142.01.T01 AT2G45950 31.788 151 91 4 4 149 13 156 1.28e-16 75.9
MsG0380014142.01.T01 AT2G45950 31.788 151 91 4 4 149 13 156 1.47e-16 75.5
MsG0380014142.01.T01 AT2G45950 31.788 151 91 4 4 149 13 156 1.47e-16 75.5
MsG0380014142.01.T01 AT2G45950 31.788 151 91 4 4 149 13 156 1.47e-16 75.5
MsG0380014142.01.T01 AT3G61415 32.000 150 88 4 6 149 15 156 5.44e-16 73.9
MsG0380014142.01.T01 AT3G61415 32.000 150 88 4 6 149 15 156 6.08e-16 73.9

Find 45 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCAGGGTGATCATCTTCT+TGG 0.232916 3:+50307066 None:intergenic
CAGACGATTATCTTAAGAAT+TGG 0.277347 3:-50306847 MsG0380014142.01.T01:CDS
TTTCACCGTCGAAACTCTTC+AGG 0.296550 3:+50307050 None:intergenic
TACCTTCGCTAACATCTTGC+CGG 0.364565 3:+50306928 None:intergenic
AGACGATTATCTTAAGAATT+GGG 0.405999 3:-50306846 MsG0380014142.01.T01:CDS
ATCCCGGTTCAGAACGTTAC+CGG 0.408425 3:-50306947 MsG0380014142.01.T01:CDS
CTGAACCGGGATTGCGTTGT+CGG 0.412527 3:+50306958 None:intergenic
CATCCTTGCTGCAAACTACT+TGG 0.414458 3:-50306780 MsG0380014142.01.T01:CDS
CGAACTTCTTCCTCTTCCTC+CGG 0.424372 3:+50306650 None:intergenic
TTGCCGGTAACGTTCTGAAC+CGG 0.428419 3:+50306944 None:intergenic
CTTTGAGGTTTCCGAGGCTG+TGG 0.449264 3:-50307026 MsG0380014142.01.T01:CDS
AACATGTTGCGAACTTCCTC+GGG 0.452049 3:+50306695 None:intergenic
TGCCGGTAACGTTCTGAACC+GGG 0.453203 3:+50306945 None:intergenic
CTTGGATATCAAGAGTCTAC+TGG 0.463162 3:-50306762 MsG0380014142.01.T01:CDS
AAACATGTTGCGAACTTCCT+CGG 0.463765 3:+50306694 None:intergenic
TCATCATGTCCGCCGCAGAC+TGG 0.476717 3:+50306729 None:intergenic
TGATTACACTCCGGAGGAAG+AGG 0.478345 3:-50306660 MsG0380014142.01.T01:CDS
CATGTCCGCCGCAGACTGGC+AGG 0.488091 3:+50306733 None:intergenic
CTTCTTGGTTGTTGAAGACA+TGG 0.497969 3:+50307081 None:intergenic
TTTCGACGGTGAAACCTTTG+AGG 0.503637 3:-50307041 MsG0380014142.01.T01:CDS
AGATAATCGTCTGATTCTGA+AGG 0.529717 3:+50306857 None:intergenic
TTAATTCATTCAAATGCCCA+TGG 0.538461 3:+50306617 None:intergenic
TGATTCGAGCGCCACAGCCT+CGG 0.547036 3:+50307015 None:intergenic
TTGTGCCGACAACGCAATCC+CGG 0.550445 3:-50306963 MsG0380014142.01.T01:CDS
TTCACCGTCGAAACTCTTCA+GGG 0.553987 3:+50307051 None:intergenic
GGAAGAGGAAGAAGTTCGAA+AGG 0.564146 3:-50306645 MsG0380014142.01.T01:CDS
CACAGCCTCGGAAACCTCAA+AGG 0.593672 3:+50307027 None:intergenic
TGATTTCGTGAAGGTTGAGA+AGG 0.594910 3:-50306819 MsG0380014142.01.T01:CDS
TAATTCATTCAAATGCCCAT+GGG 0.599690 3:+50306618 None:intergenic
TCTTACCTGCCAGTCTGCGG+CGG 0.607285 3:-50306738 MsG0380014142.01.T01:CDS
ATCACCCTGAAGAGTTTCGA+CGG 0.609201 3:-50307055 MsG0380014142.01.T01:CDS
AAGTTCGAAAGGAAAACCCA+TGG 0.609330 3:-50306634 MsG0380014142.01.T01:CDS
TGAAACCTTTGAGGTTTCCG+AGG 0.612551 3:-50307032 MsG0380014142.01.T01:CDS
GACAATCAAGCACATGATCG+AGG 0.614315 3:-50306990 MsG0380014142.01.T01:CDS
TCTGCGGCGGACATGATGAA+AGG 0.628481 3:-50306725 MsG0380014142.01.T01:CDS
CTGCAAGAAGCACGTCGAGG+TGG 0.646155 3:-50306897 MsG0380014142.01.T01:CDS
GGATCTTACCTGCCAGTCTG+CGG 0.652693 3:-50306741 MsG0380014142.01.T01:CDS
TACCGGCAAGATGTTAGCGA+AGG 0.658951 3:-50306930 MsG0380014142.01.T01:CDS
TATCCAAGTAGTTTGCAGCA+AGG 0.660134 3:+50306777 None:intergenic
AGTTCGAAAGGAAAACCCAT+GGG 0.663178 3:-50306633 MsG0380014142.01.T01:CDS
TTGGGATGCTGATTTCGTGA+AGG 0.669657 3:-50306828 MsG0380014142.01.T01:CDS
CAAGAATGATTACACTCCGG+AGG 0.672447 3:-50306666 MsG0380014142.01.T01:CDS
CATCAAGAATGATTACACTC+CGG 0.688170 3:-50306669 MsG0380014142.01.T01:CDS
GATGAAAGGAAAGACACCCG+AGG 0.735218 3:-50306711 MsG0380014142.01.T01:CDS
ATACTGCAAGAAGCACGTCG+AGG 0.756219 3:-50306900 MsG0380014142.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AGACGATTATCTTAAGAATT+GGG - Chr3:50306855-50306874 MsG0380014142.01.T01:CDS 25.0%
CAGACGATTATCTTAAGAAT+TGG - Chr3:50306854-50306873 MsG0380014142.01.T01:CDS 30.0%
AGATAATCGTCTGATTCTGA+AGG + Chr3:50306847-50306866 None:intergenic 35.0%
CATCAAGAATGATTACACTC+CGG - Chr3:50307032-50307051 MsG0380014142.01.T01:CDS 35.0%
AAACATGTTGCGAACTTCCT+CGG + Chr3:50307010-50307029 None:intergenic 40.0%
AAGTTCGAAAGGAAAACCCA+TGG - Chr3:50307067-50307086 MsG0380014142.01.T01:CDS 40.0%
AGTTCGAAAGGAAAACCCAT+GGG - Chr3:50307068-50307087 MsG0380014142.01.T01:CDS 40.0%
CTTGGATATCAAGAGTCTAC+TGG - Chr3:50306939-50306958 MsG0380014142.01.T01:CDS 40.0%
TGATTTCGTGAAGGTTGAGA+AGG - Chr3:50306882-50306901 MsG0380014142.01.T01:CDS 40.0%
! TATCCAAGTAGTTTGCAGCA+AGG + Chr3:50306927-50306946 None:intergenic 40.0%
AACATGTTGCGAACTTCCTC+GGG + Chr3:50307009-50307028 None:intergenic 45.0%
ATCACCCTGAAGAGTTTCGA+CGG - Chr3:50306646-50306665 MsG0380014142.01.T01:CDS 45.0%
CAAGAATGATTACACTCCGG+AGG - Chr3:50307035-50307054 MsG0380014142.01.T01:CDS 45.0%
CATCCTTGCTGCAAACTACT+TGG - Chr3:50306921-50306940 MsG0380014142.01.T01:CDS 45.0%
CTTCAGGGTGATCATCTTCT+TGG + Chr3:50306638-50306657 None:intergenic 45.0%
GACAATCAAGCACATGATCG+AGG - Chr3:50306711-50306730 MsG0380014142.01.T01:CDS 45.0%
GGAAGAGGAAGAAGTTCGAA+AGG - Chr3:50307056-50307075 MsG0380014142.01.T01:CDS 45.0%
TACCTTCGCTAACATCTTGC+CGG + Chr3:50306776-50306795 None:intergenic 45.0%
TTCACCGTCGAAACTCTTCA+GGG + Chr3:50306653-50306672 None:intergenic 45.0%
TTTCACCGTCGAAACTCTTC+AGG + Chr3:50306654-50306673 None:intergenic 45.0%
TTTCGACGGTGAAACCTTTG+AGG - Chr3:50306660-50306679 MsG0380014142.01.T01:CDS 45.0%
!! TGAAACCTTTGAGGTTTCCG+AGG - Chr3:50306669-50306688 MsG0380014142.01.T01:CDS 45.0%
!! TTGGGATGCTGATTTCGTGA+AGG - Chr3:50306873-50306892 MsG0380014142.01.T01:CDS 45.0%
ATCCCGGTTCAGAACGTTAC+CGG - Chr3:50306754-50306773 MsG0380014142.01.T01:CDS 50.0%
CGAACTTCTTCCTCTTCCTC+CGG + Chr3:50307054-50307073 None:intergenic 50.0%
GATGAAAGGAAAGACACCCG+AGG - Chr3:50306990-50307009 MsG0380014142.01.T01:CDS 50.0%
TACCGGCAAGATGTTAGCGA+AGG - Chr3:50306771-50306790 MsG0380014142.01.T01:CDS 50.0%
TGATTACACTCCGGAGGAAG+AGG - Chr3:50307041-50307060 MsG0380014142.01.T01:CDS 50.0%
! ATACTGCAAGAAGCACGTCG+AGG - Chr3:50306801-50306820 MsG0380014142.01.T01:CDS 50.0%
! TTGCCGGTAACGTTCTGAAC+CGG + Chr3:50306760-50306779 None:intergenic 50.0%
CACAGCCTCGGAAACCTCAA+AGG + Chr3:50306677-50306696 None:intergenic 55.0%
CTGAACCGGGATTGCGTTGT+CGG + Chr3:50306746-50306765 None:intergenic 55.0%
GGATCTTACCTGCCAGTCTG+CGG - Chr3:50306960-50306979 MsG0380014142.01.T01:CDS 55.0%
TCTGCGGCGGACATGATGAA+AGG - Chr3:50306976-50306995 MsG0380014142.01.T01:CDS 55.0%
! TGCCGGTAACGTTCTGAACC+GGG + Chr3:50306759-50306778 None:intergenic 55.0%
! TTGTGCCGACAACGCAATCC+CGG - Chr3:50306738-50306757 MsG0380014142.01.T01:CDS 55.0%
!! CTTTGAGGTTTCCGAGGCTG+TGG - Chr3:50306675-50306694 MsG0380014142.01.T01:CDS 55.0%
TCATCATGTCCGCCGCAGAC+TGG + Chr3:50306975-50306994 None:intergenic 60.0%
TCTTACCTGCCAGTCTGCGG+CGG - Chr3:50306963-50306982 MsG0380014142.01.T01:CDS 60.0%
TGATTCGAGCGCCACAGCCT+CGG + Chr3:50306689-50306708 None:intergenic 60.0%
! CTGCAAGAAGCACGTCGAGG+TGG - Chr3:50306804-50306823 MsG0380014142.01.T01:CDS 60.0%
CATGTCCGCCGCAGACTGGC+AGG + Chr3:50306971-50306990 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr3 gene 50306622 50307101 50306622 ID=MsG0380014142.01;Name=MsG0380014142.01
Chr3 mRNA 50306622 50307101 50306622 ID=MsG0380014142.01.T01;Parent=MsG0380014142.01;Name=MsG0380014142.01.T01;_AED=0.30;_eAED=0.30;_QI=0|-1|0|1|-1|1|1|0|159
Chr3 exon 50306622 50307101 50306622 ID=MsG0380014142.01.T01:exon:9694;Parent=MsG0380014142.01.T01
Chr3 CDS 50306622 50307101 50306622 ID=MsG0380014142.01.T01:cds;Parent=MsG0380014142.01.T01
Gene Sequence

>MsG0380014142.01.T01

ATGTCTTCAACAACCAAGAAGATGATCACCCTGAAGAGTTTCGACGGTGAAACCTTTGAGGTTTCCGAGGCTGTGGCGCTCGAATCACAGACAATCAAGCACATGATCGAGGATGATTGTGCCGACAACGCAATCCCGGTTCAGAACGTTACCGGCAAGATGTTAGCGAAGGTAATTGAATACTGCAAGAAGCACGTCGAGGTGGCGAGTGCTGATGAAAAACCTTCAGAATCAGACGATTATCTTAAGAATTGGGATGCTGATTTCGTGAAGGTTGAGAAGGATACGCTTTTTGAACTCATCCTTGCTGCAAACTACTTGGATATCAAGAGTCTACTGGATCTTACCTGCCAGTCTGCGGCGGACATGATGAAAGGAAAGACACCCGAGGAAGTTCGCAACATGTTTAACATCAAGAATGATTACACTCCGGAGGAAGAGGAAGAAGTTCGAAAGGAAAACCCATGGGCATTTGAATGA

Protein sequence

>MsG0380014142.01.T01

MSSTTKKMITLKSFDGETFEVSEAVALESQTIKHMIEDDCADNAIPVQNVTGKMLAKVIEYCKKHVEVASADEKPSESDDYLKNWDADFVKVEKDTLFELILAANYLDIKSLLDLTCQSAADMMKGKTPEEVRNMFNIKNDYTPEEEEEVRKENPWAFE*