AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003671.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003671.01.T01 MTR_1g068600 99.265 136 1 0 1 136 1 136 1.08e-93 266
MsG0180003671.01.T01 MTR_4g063240 85.034 147 11 2 1 136 1 147 1.84e-81 236
MsG0180003671.01.T01 MTR_4g063220 85.811 148 9 2 1 136 1 148 4.58e-78 228
MsG0180003671.01.T01 MTR_4g063990 85.811 148 9 2 1 136 1 148 4.58e-78 228
MsG0180003671.01.T01 MTR_4g063200 85.811 148 9 2 1 136 1 148 4.58e-78 228
MsG0180003671.01.T01 MTR_4g064020 85.811 148 9 2 1 136 1 148 4.58e-78 228
MsG0180003671.01.T01 MTR_1g090780 91.304 138 9 2 1 136 1 137 5.32e-78 227
MsG0180003671.01.T01 MTR_1g492910 91.304 138 9 2 1 136 1 137 6.14e-78 227
MsG0180003671.01.T01 MTR_4g071180 85.811 148 9 2 1 136 1 148 4.48e-77 225
MsG0180003671.01.T01 MTR_4g070240 85.811 148 9 2 1 136 1 148 4.48e-77 225
MsG0180003671.01.T01 MTR_4g088025 86.713 143 12 2 1 136 1 143 9.64e-76 221
MsG0180003671.01.T01 MTR_4g070245 84.459 148 11 2 1 136 1 148 1.60e-75 221
MsG0180003671.01.T01 MTR_4g063275 83.108 148 13 2 1 136 1 148 5.06e-75 220
MsG0180003671.01.T01 MTR_4g064983 84.722 144 10 2 1 132 1 144 2.63e-73 219
MsG0180003671.01.T01 MTR_7g099960 95.370 108 4 1 30 136 31 138 3.00e-64 192
MsG0180003671.01.T01 MTR_2g084480 62.921 89 33 0 47 135 136 224 1.02e-37 128
MsG0180003671.01.T01 MTR_8g069990 71.591 88 23 2 8 95 3 88 6.24e-35 117
MsG0180003671.01.T01 MTR_4g063270 90.196 51 5 0 74 124 1 51 5.97e-27 95.5
MsG0180003671.01.T01 MTR_7g114580 90.244 41 4 0 96 136 11 51 7.28e-20 77.4
MsG0180003671.01.T01 MTR_4g032777 39.744 78 47 0 43 120 33 110 1.47e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003671.01.T01 AT3G45980 83.333 150 11 2 1 136 1 150 6.24e-77 225
MsG0180003671.01.T01 AT2G37470 88.406 138 13 2 1 135 1 138 8.11e-76 221
MsG0180003671.01.T01 AT1G07790 83.784 148 12 2 1 136 1 148 2.43e-75 221
MsG0180003671.01.T01 AT2G28720 83.444 151 10 2 1 136 1 151 2.35e-74 218
MsG0180003671.01.T01 AT3G53650 87.681 138 15 2 1 136 1 138 1.62e-73 216
MsG0180003671.01.T01 AT5G59910 87.407 135 14 1 5 136 16 150 6.45e-73 214
MsG0180003671.01.T01 AT3G46030 88.372 129 12 1 11 136 17 145 1.93e-71 211
MsG0180003671.01.T01 AT5G02570 83.571 140 11 2 1 136 1 132 1.47e-68 203
MsG0180003671.01.T01 AT3G09480 80.147 136 17 1 1 136 1 126 1.28e-65 195
MsG0180003671.01.T01 AT5G22880 81.119 143 17 2 4 136 3 145 2.72e-65 195
MsG0180003671.01.T01 AT1G08170 62.353 85 32 0 49 133 151 235 9.20e-38 128

Find 32 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGAAGATGTAAATCTTAT+AGG 0.133504 1:-66389193 None:intergenic
AATTCAAGAACTCGTAAATT+TGG 0.207431 1:-66389442 None:intergenic
TTCTTTCGAGATCTTCTTCT+CGG 0.293193 1:-66389127 None:intergenic
GCTGTGCGTTTGGTTCTTCC+TGG 0.321985 1:+66389375 MsG0180003671.01.T01:CDS
TTTGAAGAAATACCTATATC+AGG 0.334896 1:-66389234 None:intergenic
CATCTTCAAGGTTCTCAAAC+AGG 0.361477 1:+66389206 MsG0180003671.01.T01:CDS
GAGATCTTCTTCTCGGCTCT+TGG 0.362586 1:-66389120 None:intergenic
CTATAAGATTTACATCTTCA+AGG 0.370441 1:+66389194 MsG0180003671.01.T01:CDS
GATTCAAACCGCTGTGCGTT+TGG 0.390239 1:+66389365 MsG0180003671.01.T01:CDS
TTTGGTTCTTCCTGGTGAAT+TGG 0.398260 1:+66389383 MsG0180003671.01.T01:CDS
TTTCTCTGCCTTGGGAGCCA+TGG 0.402280 1:-66389049 None:intergenic
AAACAGGTTCATCCTGATAT+AGG 0.421612 1:+66389222 MsG0180003671.01.T01:CDS
AGGTATTTCTTCAAAAGCTA+TGG 0.429032 1:+66389242 MsG0180003671.01.T01:CDS
CTTGTTGTAACGAGCCAATC+TGG 0.445538 1:-66389319 None:intergenic
TCCGCGGGTTTCTTCTCAGC+GGG 0.464857 1:-66389075 None:intergenic
TCTGCTGGTGATTTCTCCGC+GGG 0.470002 1:-66389090 None:intergenic
CGCTCAGGAAGCTTCCAGAT+TGG 0.475450 1:+66389305 MsG0180003671.01.T01:CDS
TGCTGTTTCTGAAGGAACTA+AGG 0.493611 1:+66389413 MsG0180003671.01.T01:CDS
GCATGTTTCGCCAATTCACC+AGG 0.516345 1:-66389393 None:intergenic
GGTATTTCTTCAAAAGCTAT+GGG 0.517184 1:+66389243 MsG0180003671.01.T01:CDS
GCGAAACATGCTGTTTCTGA+AGG 0.530754 1:+66389405 MsG0180003671.01.T01:CDS
ATTCAGATCCATGGCTCCCA+AGG 0.541897 1:+66389041 None:intergenic
GAAACCGACGATTACGTCAC+GGG 0.556515 1:+66389341 MsG0180003671.01.T01:CDS
CTCTGCTGGTGATTTCTCCG+CGG 0.560516 1:-66389091 None:intergenic
GAGAAGAAGATCTCGAAAGA+AGG 0.564931 1:+66389129 MsG0180003671.01.T01:CDS
TATCTTTGAGAAACTCGCTC+AGG 0.586744 1:+66389290 MsG0180003671.01.T01:CDS
ATCTCCCGTGACGTAATCGT+CGG 0.590908 1:-66389345 None:intergenic
CTCCGCGGGTTTCTTCTCAG+CGG 0.628471 1:-66389076 None:intergenic
AAGAAGATCTCGAAAGAAGG+AGG 0.628797 1:+66389132 MsG0180003671.01.T01:CDS
AGAAACCGACGATTACGTCA+CGG 0.672005 1:+66389340 MsG0180003671.01.T01:CDS
ACCCGCTGAGAAGAAACCCG+CGG 0.690098 1:+66389074 MsG0180003671.01.T01:CDS
AGGAAGAACCAAACGCACAG+CGG 0.757762 1:-66389373 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CTATAAGATTTACATCTTCA+AGG + Chr1:66389194-66389213 MsG0180003671.01.T01:CDS 25.0%
! CTTGAAGATGTAAATCTTAT+AGG - Chr1:66389196-66389215 None:intergenic 25.0%
! TTTGAAGAAATACCTATATC+AGG - Chr1:66389237-66389256 None:intergenic 25.0%
AGGTATTTCTTCAAAAGCTA+TGG + Chr1:66389242-66389261 MsG0180003671.01.T01:CDS 30.0%
GGTATTTCTTCAAAAGCTAT+GGG + Chr1:66389243-66389262 MsG0180003671.01.T01:CDS 30.0%
AAACAGGTTCATCCTGATAT+AGG + Chr1:66389222-66389241 MsG0180003671.01.T01:CDS 35.0%
TTCTTTCGAGATCTTCTTCT+CGG - Chr1:66389130-66389149 None:intergenic 35.0%
AAGAAGATCTCGAAAGAAGG+AGG + Chr1:66389132-66389151 MsG0180003671.01.T01:CDS 40.0%
CATCTTCAAGGTTCTCAAAC+AGG + Chr1:66389206-66389225 MsG0180003671.01.T01:CDS 40.0%
GAGAAGAAGATCTCGAAAGA+AGG + Chr1:66389129-66389148 MsG0180003671.01.T01:CDS 40.0%
! TATCTTTGAGAAACTCGCTC+AGG + Chr1:66389290-66389309 MsG0180003671.01.T01:CDS 40.0%
!! TGCTGTTTCTGAAGGAACTA+AGG + Chr1:66389413-66389432 MsG0180003671.01.T01:CDS 40.0%
!! TTTGGTTCTTCCTGGTGAAT+TGG + Chr1:66389383-66389402 MsG0180003671.01.T01:CDS 40.0%
AGAAACCGACGATTACGTCA+CGG + Chr1:66389340-66389359 MsG0180003671.01.T01:CDS 45.0%
! CTTGTTGTAACGAGCCAATC+TGG - Chr1:66389322-66389341 None:intergenic 45.0%
!! GCGAAACATGCTGTTTCTGA+AGG + Chr1:66389405-66389424 MsG0180003671.01.T01:CDS 45.0%
AGGAAGAACCAAACGCACAG+CGG - Chr1:66389376-66389395 None:intergenic 50.0%
ATCTCCCGTGACGTAATCGT+CGG - Chr1:66389348-66389367 None:intergenic 50.0%
GAAACCGACGATTACGTCAC+GGG + Chr1:66389341-66389360 MsG0180003671.01.T01:CDS 50.0%
GAGATCTTCTTCTCGGCTCT+TGG - Chr1:66389123-66389142 None:intergenic 50.0%
GATTCAAACCGCTGTGCGTT+TGG + Chr1:66389365-66389384 MsG0180003671.01.T01:CDS 50.0%
GCATGTTTCGCCAATTCACC+AGG - Chr1:66389396-66389415 None:intergenic 50.0%
!! GCTCTTGGCTTTTTCTCTGC+TGG - Chr1:66389108-66389127 None:intergenic 50.0%
!!! AGCGGGTTTTTTCTCTGCCT+TGG - Chr1:66389061-66389080 None:intergenic 50.0%
!!! GCGGGTTTTTTCTCTGCCTT+GGG - Chr1:66389060-66389079 None:intergenic 50.0%
CGCTCAGGAAGCTTCCAGAT+TGG + Chr1:66389305-66389324 MsG0180003671.01.T01:CDS 55.0%
CTCTGCTGGTGATTTCTCCG+CGG - Chr1:66389094-66389113 None:intergenic 55.0%
TCTGCTGGTGATTTCTCCGC+GGG - Chr1:66389093-66389112 None:intergenic 55.0%
!! GCTGTGCGTTTGGTTCTTCC+TGG + Chr1:66389375-66389394 MsG0180003671.01.T01:CDS 55.0%
ACCCGCTGAGAAGAAACCCG+CGG + Chr1:66389074-66389093 MsG0180003671.01.T01:CDS 60.0%
CTCCGCGGGTTTCTTCTCAG+CGG - Chr1:66389079-66389098 None:intergenic 60.0%
TCCGCGGGTTTCTTCTCAGC+GGG - Chr1:66389078-66389097 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 66389051 66389461 66389051 ID=MsG0180003671.01;Name=MsG0180003671.01
Chr1 mRNA 66389051 66389461 66389051 ID=MsG0180003671.01.T01;Parent=MsG0180003671.01;Name=MsG0180003671.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|136
Chr1 exon 66389051 66389461 66389051 ID=MsG0180003671.01.T01:exon:29878;Parent=MsG0180003671.01.T01
Chr1 CDS 66389051 66389461 66389051 ID=MsG0180003671.01.T01:cds;Parent=MsG0180003671.01.T01
Gene Sequence

>MsG0180003671.01.T01

ATGGCTCCCAAGGCAGAGAAAAAACCCGCTGAGAAGAAACCCGCGGAGAAATCACCAGCAGAGAAAAAGCCAAGAGCCGAGAAGAAGATCTCGAAAGAAGGAGGCGATAAGAAGAAGAAGCGCGTGAAGAAGAGCGTTGAAACCTATAAGATTTACATCTTCAAGGTTCTCAAACAGGTTCATCCTGATATAGGTATTTCTTCAAAAGCTATGGGAATTATGAACAGTTTCATCAATGATATCTTTGAGAAACTCGCTCAGGAAGCTTCCAGATTGGCTCGTTACAACAAGAAACCGACGATTACGTCACGGGAGATTCAAACCGCTGTGCGTTTGGTTCTTCCTGGTGAATTGGCGAAACATGCTGTTTCTGAAGGAACTAAGGCTGTTACCAAATTTACGAGTTCTTGA

Protein sequence

>MsG0180003671.01.T01

MAPKAEKKPAEKKPAEKSPAEKKPRAEKKISKEGGDKKKKRVKKSVETYKIYIFKVLKQVHPDIGISSKAMGIMNSFINDIFEKLAQEASRLARYNKKPTITSREIQTAVRLVLPGELAKHAVSEGTKAVTKFTSS*