AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002100.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002100.01.T01 MTR_1g048960 97.917 96 2 0 1 96 1 96 1.44e-65 192
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002100.01.T01 AT1G72020 69.072 97 29 1 1 96 1 97 8.09e-45 140

Find 43 sgRNAs with CRISPR-Local

Find 179 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACAATAATCAAATGGATT+TGG 0.226650 1:+32894724 MsG0180002100.01.T01:three_prime_UTR
GGAATCATATTGCATTAATA+TGG 0.232140 1:-32895011 None:intergenic
AACAATAATCAAATGGATTT+GGG 0.236605 1:+32894725 MsG0180002100.01.T01:three_prime_UTR
CTAGACTAGCATATTGGTTT+TGG 0.253646 1:+32894752 MsG0180002100.01.T01:three_prime_UTR
GTGAGGGATTTGACCTGATT+TGG 0.301994 1:-32892729 None:intergenic
AATGTCCAAGCACAAATATT+TGG 0.313332 1:-32894868 None:intergenic
CTTGGACAAGATGAAGTATC+AGG 0.339393 1:+32894580 MsG0180002100.01.T01:CDS
TGATTATTACAAGAGATATA+TGG 0.342391 1:-32894831 None:intergenic
TTGATGAAGTTGGTTATTGC+TGG 0.377823 1:+32894957 MsG0180002100.01.T01:three_prime_UTR
CATTTGATTATTGTTACAAA+TGG 0.377897 1:-32894717 None:intergenic
AATAGAGGATTTCATTCAAC+TGG 0.390216 1:+32894488 MsG0180002100.01.T01:CDS
GGGATCCTAGACTAGCATAT+TGG 0.403278 1:+32894746 MsG0180002100.01.T01:three_prime_UTR
CTTCAGTAGTTTGATGAAGT+TGG 0.410222 1:+32894947 MsG0180002100.01.T01:three_prime_UTR
GCATATACTCTGGCTCACTA+TGG 0.425528 1:-32894538 None:intergenic
GAATCCTCCATCTCCAAATC+AGG 0.426967 1:+32892716 MsG0180002100.01.T01:CDS
CAGCAAAAGAAGACAGCATC+TGG 0.440272 1:+32894677 MsG0180002100.01.T01:CDS
TTGTTAAGCTTGGCGAAACT+AGG 0.466604 1:+32892575 MsG0180002100.01.T01:five_prime_UTR
GAAACACAAATTTCTTTCGA+AGG 0.478060 1:+32892612 MsG0180002100.01.T01:five_prime_UTR
CATTTGTAACAATAATCAAA+TGG 0.478844 1:+32894718 MsG0180002100.01.T01:three_prime_UTR
AGCTTTCAGCATTGGTGTTG+CGG 0.482260 1:+32894631 MsG0180002100.01.T01:CDS
AAGAGGATGGGAGGCGGACA+TGG 0.489860 1:+32894515 MsG0180002100.01.T01:CDS
ATATGGAATGAAACAAATGA+GGG 0.495115 1:-32894994 None:intergenic
GTGTTCACAGCTTTCAGCAT+TGG 0.501579 1:+32894623 MsG0180002100.01.T01:CDS
TTCAACTGGCGTGAAGAGGA+TGG 0.515922 1:+32894502 MsG0180002100.01.T01:CDS
TTGACCTGATTTGGAGATGG+AGG 0.515967 1:-32892720 None:intergenic
CAAAACCAATATGCTAGTCT+AGG 0.520108 1:-32894751 None:intergenic
AATATGGAATGAAACAAATG+AGG 0.526846 1:-32894995 None:intergenic
TGCATATCACATGTACAACT+TGG 0.529028 1:+32894562 MsG0180002100.01.T01:CDS
TGTTAAGCTTGGCGAAACTA+GGG 0.529654 1:+32892576 MsG0180002100.01.T01:five_prime_UTR
GATTTGACCTGATTTGGAGA+TGG 0.541306 1:-32892723 None:intergenic
GGCGTGAAGAGGATGGGAGG+CGG 0.552929 1:+32894509 MsG0180002100.01.T01:CDS
CTTGAAAGGAAATATAGACT+TGG 0.553076 1:+32894898 MsG0180002100.01.T01:three_prime_UTR
TTCATTCAACTGGCGTGAAG+AGG 0.553273 1:+32894498 MsG0180002100.01.T01:CDS
TCAACTGGCGTGAAGAGGAT+GGG 0.574066 1:+32894503 MsG0180002100.01.T01:CDS
TGGAAAATGACTGCATACAC+AGG 0.585917 1:-32894656 None:intergenic
TATGTTGTGACAGTGAATAG+AGG 0.607805 1:+32894473 MsG0180002100.01.T01:intron
TTGGACAAGATGAAGTATCA+GGG 0.612137 1:+32894581 MsG0180002100.01.T01:CDS
ACAATAATCAAATGGATTTG+GGG 0.612392 1:+32894726 MsG0180002100.01.T01:three_prime_UTR
TGATATGCATGCATATACTC+TGG 0.624731 1:-32894548 None:intergenic
ATAACGATGGCATTGAACAA+CGG 0.641834 1:+32892659 MsG0180002100.01.T01:exon
ACTGGCGTGAAGAGGATGGG+AGG 0.672984 1:+32894506 MsG0180002100.01.T01:CDS
TATGGAATGAAACAAATGAG+GGG 0.691924 1:-32894993 None:intergenic
AATCGAATTTGCGATAACGA+TGG 0.695476 1:+32892646 MsG0180002100.01.T01:exon

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGATTTGATTTATTAATAAA+TGG + Chr1:32892859-32892878 MsG0180002100.01.T01:intron 10.0%
!! TTGATTTATTAATAAATGGA+GGG + Chr1:32892863-32892882 MsG0180002100.01.T01:intron 15.0%
!!! AGTTTTATATATGTATTACA+TGG + Chr1:32893560-32893579 MsG0180002100.01.T01:intron 15.0%
!!! GTTTTATATATGTATTACAT+GGG + Chr1:32893561-32893580 MsG0180002100.01.T01:intron 15.0%
!!! TTACATTAGGTTTTTTATTT+GGG + Chr1:32892822-32892841 MsG0180002100.01.T01:intron 15.0%
!!! TTTGATTTATTAATAAATGG+AGG + Chr1:32892862-32892881 MsG0180002100.01.T01:intron 15.0%
!!! TTTGTTTTTACTTTCAATTT+CGG + Chr1:32892799-32892818 MsG0180002100.01.T01:intron 15.0%
!! AATTAACGGTTTAGATTAAA+TGG + Chr1:32893650-32893669 MsG0180002100.01.T01:intron 20.0%
!! CATTTGATTATTGTTACAAA+TGG - Chr1:32894720-32894739 None:intergenic 20.0%
!! CATTTGTAACAATAATCAAA+TGG + Chr1:32894718-32894737 MsG0180002100.01.T01:three_prime_UTR 20.0%
!! GAATATTATTAATGATACTC+TGG - Chr1:32893530-32893549 None:intergenic 20.0%
!! TGATTATTACAAGAGATATA+TGG - Chr1:32894834-32894853 None:intergenic 20.0%
!! TTATTTGACTAATGAACATA+TGG + Chr1:32893472-32893491 MsG0180002100.01.T01:intron 20.0%
!!! AACAATAATCAAATGGATTT+GGG + Chr1:32894725-32894744 MsG0180002100.01.T01:three_prime_UTR 20.0%
!!! GTTACATTAGGTTTTTTATT+TGG + Chr1:32892821-32892840 MsG0180002100.01.T01:intron 20.0%
!!! GTTCATGTTTAATTTTCATA+TGG + Chr1:32894803-32894822 MsG0180002100.01.T01:three_prime_UTR 20.0%
!!! TAAAACAATTTTGTTGTCTA+AGG + Chr1:32893043-32893062 MsG0180002100.01.T01:intron 20.0%
!!! TAACAATAATCAAATGGATT+TGG + Chr1:32894724-32894743 MsG0180002100.01.T01:three_prime_UTR 20.0%
!!! TACATTAGGTTTTTTATTTG+GGG + Chr1:32892823-32892842 MsG0180002100.01.T01:intron 20.0%
! AAAGAATATGAAGGGAAAAA+AGG + Chr1:32893252-32893271 MsG0180002100.01.T01:intron 25.0%
! AAATTAACATGGATCTTAGA+GGG + Chr1:32893192-32893211 MsG0180002100.01.T01:intron 25.0%
! AAGACCTTAGATAAATTTGA+AGG - Chr1:32893368-32893387 None:intergenic 25.0%
! AATATGGAATGAAACAAATG+AGG - Chr1:32894998-32895017 None:intergenic 25.0%
! AGACCTTAGATAAATTTGAA+GGG - Chr1:32893367-32893386 None:intergenic 25.0%
! ATATGGAATGAAACAAATGA+GGG - Chr1:32894997-32895016 None:intergenic 25.0%
! GGAATCATATTGCATTAATA+TGG - Chr1:32895014-32895033 None:intergenic 25.0%
! GTCATTGGATTAACAATTAA+CGG + Chr1:32893636-32893655 MsG0180002100.01.T01:intron 25.0%
! GTTAAATACAGTTCTTGATT+GGG + Chr1:32894263-32894282 MsG0180002100.01.T01:intron 25.0%
! TGTTAAATACAGTTCTTGAT+TGG + Chr1:32894262-32894281 MsG0180002100.01.T01:intron 25.0%
!! ACAATAATCAAATGGATTTG+GGG + Chr1:32894726-32894745 MsG0180002100.01.T01:three_prime_UTR 25.0%
!! ATCAATATTTTCAATGCATC+GGG - Chr1:32892991-32893010 None:intergenic 25.0%
!! CATCAATATTTTCAATGCAT+CGG - Chr1:32892992-32893011 None:intergenic 25.0%
!!! ACATTAGGTTTTTTATTTGG+GGG + Chr1:32892824-32892843 MsG0180002100.01.T01:intron 25.0%
!!! ATTTTGTACATTTTGATCCA+AGG - Chr1:32893803-32893822 None:intergenic 25.0%
!!! GTTTTGATTCCAAATTAACA+TGG + Chr1:32893181-32893200 MsG0180002100.01.T01:intron 25.0%
!!! TGATGTTTTAAATTGCCATT+AGG + Chr1:32892899-32892918 MsG0180002100.01.T01:intron 25.0%
!!! TTGTGGTTTTGTTTATATTG+TGG + Chr1:32892922-32892941 MsG0180002100.01.T01:intron 25.0%
AAACAAGCACGAATATTCAA+TGG + Chr1:32893942-32893961 MsG0180002100.01.T01:intron 30.0%
AAAGAATTCCTGTTAGTCAT+TGG + Chr1:32893621-32893640 MsG0180002100.01.T01:intron 30.0%
AAAGAGTCGAAAGAATATGA+AGG + Chr1:32893243-32893262 MsG0180002100.01.T01:intron 30.0%
AAATCTTCCTCAACAGTAAT+CGG - Chr1:32892774-32892793 None:intergenic 30.0%
AACAAGCACGAATATTCAAT+GGG + Chr1:32893943-32893962 MsG0180002100.01.T01:intron 30.0%
AAGAGTCGAAAGAATATGAA+GGG + Chr1:32893244-32893263 MsG0180002100.01.T01:intron 30.0%
AATAGAGGATTTCATTCAAC+TGG + Chr1:32894488-32894507 MsG0180002100.01.T01:CDS 30.0%
AATGTCCAAGCACAAATATT+TGG - Chr1:32894871-32894890 None:intergenic 30.0%
AGTCATCGGATTAAAATAGA+TGG - Chr1:32894339-32894358 None:intergenic 30.0%
CAAATTAACATGGATCTTAG+AGG + Chr1:32893191-32893210 MsG0180002100.01.T01:intron 30.0%
CATTAAATGCAGCAAAAACA+TGG - Chr1:32893423-32893442 None:intergenic 30.0%
CTTGAAAGGAAATATAGACT+TGG + Chr1:32894898-32894917 MsG0180002100.01.T01:three_prime_UTR 30.0%
CTTTCAATTTCGGTTACATT+AGG + Chr1:32892809-32892828 MsG0180002100.01.T01:intron 30.0%
GAAACACAAATTTCTTTCGA+AGG + Chr1:32892612-32892631 MsG0180002100.01.T01:five_prime_UTR 30.0%
TAAACAAAACCACAACCTAA+TGG - Chr1:32892917-32892936 None:intergenic 30.0%
TATGGAATGAAACAAATGAG+GGG - Chr1:32894996-32895015 None:intergenic 30.0%
TGATTGGGCATACAAAATAT+TGG + Chr1:32894278-32894297 MsG0180002100.01.T01:intron 30.0%
TTCAGAATTTAACTCCTAGA+AGG + Chr1:32893966-32893985 MsG0180002100.01.T01:intron 30.0%
TTGTTAATCCAATGACTAAC+AGG - Chr1:32893632-32893651 None:intergenic 30.0%
TTTCCTAGACATCTTAAAAC+AGG - Chr1:32894415-32894434 None:intergenic 30.0%
! AAAACATTCTTTGTTGGTTG+TGG + Chr1:32894164-32894183 MsG0180002100.01.T01:intron 30.0%
! AAACATTCTTTGTTGGTTGT+GGG + Chr1:32894165-32894184 MsG0180002100.01.T01:intron 30.0%
! CAGATACATTTTGTTAAGCT+TGG + Chr1:32892565-32892584 MsG0180002100.01.T01:five_prime_UTR 30.0%
! GAAGATAAAAGTGTAATGTG+AGG - Chr1:32893688-32893707 None:intergenic 30.0%
! GGACATTTTTATGTCTTGAA+AGG + Chr1:32894884-32894903 MsG0180002100.01.T01:three_prime_UTR 30.0%
! TTTAAATTGCCATTAGGTTG+TGG + Chr1:32892905-32892924 MsG0180002100.01.T01:intron 30.0%
! TTTTGCCAAATATTTGTGCT+TGG + Chr1:32894863-32894882 MsG0180002100.01.T01:three_prime_UTR 30.0%
! TTTTTATGTGGAAACGAAAG+AGG - Chr1:32894370-32894389 None:intergenic 30.0%
!! CTCTAAGATCCATGTTAATT+TGG - Chr1:32893193-32893212 None:intergenic 30.0%
!!! TGAGATCGACTTTTTTTATG+TGG - Chr1:32894382-32894401 None:intergenic 30.0%
AAAGCTAACAACATCACCAT+TGG + Chr1:32894192-32894211 MsG0180002100.01.T01:intron 35.0%
AAGAGGTTCTTGTTAGTCAT+CGG - Chr1:32894353-32894372 None:intergenic 35.0%
AATCGAATTTGCGATAACGA+TGG + Chr1:32892646-32892665 MsG0180002100.01.T01:exon 35.0%
AGCAGACTAATGTATGAGAA+GGG + Chr1:32893087-32893106 MsG0180002100.01.T01:intron 35.0%
ATAAATTTGAAGGGCGACTT+AGG - Chr1:32893358-32893377 None:intergenic 35.0%
ATACAAAATATTGGCGTCTG+GGG + Chr1:32894287-32894306 MsG0180002100.01.T01:intron 35.0%
CAACTGAACTAAGAAACAGT+AGG - Chr1:32893396-32893415 None:intergenic 35.0%
CATACAAAATATTGGCGTCT+GGG + Chr1:32894286-32894305 MsG0180002100.01.T01:intron 35.0%
CGGATTGAACATGAAAGAAA+AGG - Chr1:32892754-32892773 None:intergenic 35.0%
CTTCAGTAGTTTGATGAAGT+TGG + Chr1:32894947-32894966 MsG0180002100.01.T01:three_prime_UTR 35.0%
GAACATGAAAGAAAAGGTGA+GGG - Chr1:32892748-32892767 None:intergenic 35.0%
TAAATTTGAAGGGCGACTTA+GGG - Chr1:32893357-32893376 None:intergenic 35.0%
TAGCAGACTAATGTATGAGA+AGG + Chr1:32893086-32893105 MsG0180002100.01.T01:intron 35.0%
TATGTTGTGACAGTGAATAG+AGG + Chr1:32894473-32894492 MsG0180002100.01.T01:intron 35.0%
TCGCCCTTCAAATTTATCTA+AGG + Chr1:32893361-32893380 MsG0180002100.01.T01:intron 35.0%
TGAACATGAAAGAAAAGGTG+AGG - Chr1:32892749-32892768 None:intergenic 35.0%
TGATATGCATGCATATACTC+TGG - Chr1:32894551-32894570 None:intergenic 35.0%
TGATTCATGCAAAACATCCA+AGG - Chr1:32893865-32893884 None:intergenic 35.0%
TGCATATCACATGTACAACT+TGG + Chr1:32894562-32894581 MsG0180002100.01.T01:CDS 35.0%
! AATGTATGAGAAGGGTATGA+TGG + Chr1:32893095-32893114 MsG0180002100.01.T01:intron 35.0%
! ATAACGATGGCATTGAACAA+CGG + Chr1:32892659-32892678 MsG0180002100.01.T01:exon 35.0%
! ATCTTAGAGGGCTTTTGTTA+TGG + Chr1:32893204-32893223 MsG0180002100.01.T01:intron 35.0%
! CAAAACCAATATGCTAGTCT+AGG - Chr1:32894754-32894773 None:intergenic 35.0%
! TTGATGAAGTTGGTTATTGC+TGG + Chr1:32894957-32894976 MsG0180002100.01.T01:three_prime_UTR 35.0%
! TTGGACAAGATGAAGTATCA+GGG + Chr1:32894581-32894600 MsG0180002100.01.T01:CDS 35.0%
!! GTACATTTTGATCCAAGGTT+GGG - Chr1:32893798-32893817 None:intergenic 35.0%
!! TGTACATTTTGATCCAAGGT+TGG - Chr1:32893799-32893818 None:intergenic 35.0%
!! TTGAGAAGTATCAGTATGCA+AGG - Chr1:32893773-32893792 None:intergenic 35.0%
!!! ATGGGTATTTTGATCCAAGA+TGG - Chr1:32893742-32893761 None:intergenic 35.0%
!!! CTAGACTAGCATATTGGTTT+TGG + Chr1:32894752-32894771 MsG0180002100.01.T01:three_prime_UTR 35.0%
!!! TCTCCTGTTTTAAGATGTCT+AGG + Chr1:32894409-32894428 MsG0180002100.01.T01:intron 35.0%
AAGATGGCAATGACAACAAG+AGG - Chr1:32893726-32893745 None:intergenic 40.0%
AGTATGCAAGGATGGTGATA+TGG - Chr1:32893761-32893780 None:intergenic 40.0%
ATGCAAAACATCCAAGGATG+GGG - Chr1:32893859-32893878 None:intergenic 40.0%
CATGCAAAACATCCAAGGAT+GGG - Chr1:32893860-32893879 None:intergenic 40.0%
CCACAAGTAGAGCTATGTTA+TGG + Chr1:32893018-32893037 MsG0180002100.01.T01:intron 40.0%
GCATACAAAATATTGGCGTC+TGG + Chr1:32894285-32894304 MsG0180002100.01.T01:intron 40.0%
GGCTGGAAAACATTCTTTGT+TGG + Chr1:32894158-32894177 MsG0180002100.01.T01:intron 40.0%
GTATGCAAGGATGGTGATAT+GGG - Chr1:32893760-32893779 None:intergenic 40.0%
GTTAGTGGCATAGTTGTATG+TGG + Chr1:32894043-32894062 MsG0180002100.01.T01:intron 40.0%
GTTCAATCCGATTACTGTTG+AGG + Chr1:32892764-32892783 MsG0180002100.01.T01:intron 40.0%
TATGTTTGTGAGATTGCACG+AGG - Chr1:32893831-32893850 None:intergenic 40.0%
TCATGCAAAACATCCAAGGA+TGG - Chr1:32893861-32893880 None:intergenic 40.0%
TCTCTGACTTCTAACCTTCT+AGG - Chr1:32893983-32894002 None:intergenic 40.0%
TGGAAAATGACTGCATACAC+AGG - Chr1:32894659-32894678 None:intergenic 40.0%
TGTTAAGCTTGGCGAAACTA+GGG + Chr1:32892576-32892595 MsG0180002100.01.T01:five_prime_UTR 40.0%
TTGTTAAGCTTGGCGAAACT+AGG + Chr1:32892575-32892594 MsG0180002100.01.T01:five_prime_UTR 40.0%
! CCATAACATAGCTCTACTTG+TGG - Chr1:32893021-32893040 None:intergenic 40.0%
! CTTGGACAAGATGAAGTATC+AGG + Chr1:32894580-32894599 MsG0180002100.01.T01:CDS 40.0%
! GAAGTATCAGTATGCAAGGA+TGG - Chr1:32893769-32893788 None:intergenic 40.0%
! TCTTGTTGTCATTGCCATCT+TGG + Chr1:32893725-32893744 MsG0180002100.01.T01:intron 40.0%
! TTTAGAGCCTCAAAAGCAGT+TGG - Chr1:32894107-32894126 None:intergenic 40.0%
!! CTTGGAATGTCCTGTTTTTC+AGG + Chr1:32894916-32894935 MsG0180002100.01.T01:three_prime_UTR 40.0%
!! CTTTTGTTATGGAATCGGTG+TGG + Chr1:32893215-32893234 MsG0180002100.01.T01:intron 40.0%
!! GAGGGCTTTTGTTATGGAAT+CGG + Chr1:32893210-32893229 MsG0180002100.01.T01:intron 40.0%
!! GATTTGACCTGATTTGGAGA+TGG - Chr1:32892726-32892745 None:intergenic 40.0%
AACATACAGAACCCCATCCT+TGG + Chr1:32893845-32893864 MsG0180002100.01.T01:intron 45.0%
AACCAGCATCCCCATTTCAA+TGG + Chr1:32894126-32894145 MsG0180002100.01.T01:intron 45.0%
ACCAGCATCCCCATTTCAAT+GGG + Chr1:32894127-32894146 MsG0180002100.01.T01:intron 45.0%
AGAACCACCACCTGAAAAAC+AGG - Chr1:32894929-32894948 None:intergenic 45.0%
AGGGGATGAATCAGTTTCAG+TGG + Chr1:32894235-32894254 MsG0180002100.01.T01:intron 45.0%
AGTGGCATAGTTGTATGTGG+TGG + Chr1:32894046-32894065 MsG0180002100.01.T01:intron 45.0%
CAGCAAAAGAAGACAGCATC+TGG + Chr1:32894677-32894696 MsG0180002100.01.T01:CDS 45.0%
GAATCCTCCATCTCCAAATC+AGG + Chr1:32892716-32892735 MsG0180002100.01.T01:CDS 45.0%
GCGTGAGAGAGTTATATGCT+CGG + Chr1:32894013-32894032 MsG0180002100.01.T01:intron 45.0%
GCTAACAACATCACCATTGG+TGG + Chr1:32894195-32894214 MsG0180002100.01.T01:intron 45.0%
GGGATCCTAGACTAGCATAT+TGG + Chr1:32894746-32894765 MsG0180002100.01.T01:three_prime_UTR 45.0%
GTGAGGGATTTGACCTGATT+TGG - Chr1:32892732-32892751 None:intergenic 45.0%
GTGTTCACAGCTTTCAGCAT+TGG + Chr1:32894623-32894642 MsG0180002100.01.T01:CDS 45.0%
TACTTCTCAAGACCCAACCT+TGG + Chr1:32893783-32893802 MsG0180002100.01.T01:intron 45.0%
TTCATTCAACTGGCGTGAAG+AGG + Chr1:32894498-32894517 MsG0180002100.01.T01:CDS 45.0%
TTTGTGAGATTGCACGAGGA+TGG - Chr1:32893827-32893846 None:intergenic 45.0%
! ACAACATCACCATTGGTGGT+AGG + Chr1:32894199-32894218 MsG0180002100.01.T01:intron 45.0%
! CAGATGCTGTCTTCTTTTGC+TGG - Chr1:32894679-32894698 None:intergenic 45.0%
! GCATATACTCTGGCTCACTA+TGG - Chr1:32894541-32894560 None:intergenic 45.0%
!! AACCCTGTTGGTAGCTTGAT+GGG - Chr1:32894079-32894098 None:intergenic 45.0%
!! AGCTTTCAGCATTGGTGTTG+CGG + Chr1:32894631-32894650 MsG0180002100.01.T01:CDS 45.0%
!! TTCCTCTCCAACTGCTTTTG+AGG + Chr1:32894097-32894116 MsG0180002100.01.T01:intron 45.0%
!! TTGACCTGATTTGGAGATGG+AGG - Chr1:32892723-32892742 None:intergenic 45.0%
!!! ATGTCCTGTTTTTCAGGTGG+TGG + Chr1:32894922-32894941 MsG0180002100.01.T01:three_prime_UTR 45.0%
!!! GGAATGTCCTGTTTTTCAGG+TGG + Chr1:32894919-32894938 MsG0180002100.01.T01:three_prime_UTR 45.0%
AGCCTCAAAAGCAGTTGGAG+AGG - Chr1:32894102-32894121 None:intergenic 50.0%
ATCCCCATCAAGCTACCAAC+AGG + Chr1:32894073-32894092 MsG0180002100.01.T01:intron 50.0%
ATGCTCGGCATCTTCGTTAG+TGG + Chr1:32894028-32894047 MsG0180002100.01.T01:intron 50.0%
CCAGCATCCCCATTTCAATG+GGG + Chr1:32894128-32894147 MsG0180002100.01.T01:intron 50.0%
CCATTTCAATGGGGTGGCAT+CGG + Chr1:32894137-32894156 MsG0180002100.01.T01:intron 50.0%
CGACTTAGGGTAGAGAAGGT+CGG - Chr1:32893344-32893363 None:intergenic 50.0%
CGATGCCACCCCATTGAAAT+GGG - Chr1:32894139-32894158 None:intergenic 50.0%
GATGCCACCCCATTGAAATG+GGG - Chr1:32894138-32894157 None:intergenic 50.0%
GGCATAGTTGTATGTGGTGG+TGG + Chr1:32894049-32894068 MsG0180002100.01.T01:intron 50.0%
TCAACTGGCGTGAAGAGGAT+GGG + Chr1:32894503-32894522 MsG0180002100.01.T01:CDS 50.0%
TCCCCATCAAGCTACCAACA+GGG + Chr1:32894074-32894093 MsG0180002100.01.T01:intron 50.0%
TTCAACTGGCGTGAAGAGGA+TGG + Chr1:32894502-32894521 MsG0180002100.01.T01:CDS 50.0%
! ACTGATTCATCCCCTCCCAT+AGG - Chr1:32894230-32894249 None:intergenic 50.0%
! ATCACCATTGGTGGTAGGTG+TGG + Chr1:32894204-32894223 MsG0180002100.01.T01:intron 50.0%
!! ACCCTGTTGGTAGCTTGATG+GGG - Chr1:32894078-32894097 None:intergenic 50.0%
!! GAACCCTGTTGGTAGCTTGA+TGG - Chr1:32894080-32894099 None:intergenic 50.0%
AGGGCGACTTAGGGTAGAGA+AGG - Chr1:32893348-32893367 None:intergenic 55.0%
CAGTTGGAGAGGAACCCTGT+TGG - Chr1:32894091-32894110 None:intergenic 55.0%
CCCCATTGAAATGGGGATGC+TGG - Chr1:32894131-32894150 None:intergenic 55.0%
CCGATGCCACCCCATTGAAA+TGG - Chr1:32894140-32894159 None:intergenic 55.0%
GCATCCCCATTTCAATGGGG+TGG + Chr1:32894131-32894150 MsG0180002100.01.T01:intron 55.0%
TAGGCCACACCTACCACCAA+TGG - Chr1:32894211-32894230 None:intergenic 55.0%
TGGTGGTAGGTGTGGCCTAT+GGG + Chr1:32894212-32894231 MsG0180002100.01.T01:intron 55.0%
! TTGGTGGTAGGTGTGGCCTA+TGG + Chr1:32894211-32894230 MsG0180002100.01.T01:intron 55.0%
AAGAGGATGGGAGGCGGACA+TGG + Chr1:32894515-32894534 MsG0180002100.01.T01:CDS 60.0%
ACTGGCGTGAAGAGGATGGG+AGG + Chr1:32894506-32894525 MsG0180002100.01.T01:CDS 60.0%
GGTAGGTGTGGCCTATGGGA+GGG + Chr1:32894216-32894235 MsG0180002100.01.T01:intron 60.0%
GTAGGTGTGGCCTATGGGAG+GGG + Chr1:32894217-32894236 MsG0180002100.01.T01:intron 60.0%
TGGTAGGTGTGGCCTATGGG+AGG + Chr1:32894215-32894234 MsG0180002100.01.T01:intron 60.0%
!! TTCAATGGGGTGGCATCGGC+TGG + Chr1:32894141-32894160 MsG0180002100.01.T01:intron 60.0%
GGCGTGAAGAGGATGGGAGG+CGG + Chr1:32894509-32894528 MsG0180002100.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 32892562 32895039 32892562 ID=MsG0180002100.01;Name=MsG0180002100.01
Chr1 mRNA 32892562 32895039 32892562 ID=MsG0180002100.01.T01;Parent=MsG0180002100.01;Name=MsG0180002100.01.T01;_AED=0.49;_eAED=0.49;_QI=103|1|1|1|1|1|2|336|96
Chr1 exon 32894486 32895039 32894486 ID=MsG0180002100.01.T01:exon:7482;Parent=MsG0180002100.01.T01
Chr1 exon 32892562 32892737 32892562 ID=MsG0180002100.01.T01:exon:7481;Parent=MsG0180002100.01.T01
Chr1 five_prime_UTR 32892562 32892664 32892562 ID=MsG0180002100.01.T01:five_prime_utr;Parent=MsG0180002100.01.T01
Chr1 CDS 32892665 32892737 32892665 ID=MsG0180002100.01.T01:cds;Parent=MsG0180002100.01.T01
Chr1 CDS 32894486 32894703 32894486 ID=MsG0180002100.01.T01:cds;Parent=MsG0180002100.01.T01
Chr1 three_prime_UTR 32894704 32895039 32894704 ID=MsG0180002100.01.T01:three_prime_utr;Parent=MsG0180002100.01.T01
Gene Sequence

>MsG0180002100.01.T01

ATGGCATTGAACAACGGTCTCAGATCTGCTGTGAAGCTAATTGCTTCTTCTGAATCCTCCATCTCCAAATCAGTGAATAGAGGATTTCATTCAACTGGCGTGAAGAGGATGGGAGGCGGACATGGCCATAGTGAGCCAGAGTATATGCATGCATATCACATGTACAACTTGGACAAGATGAAGTATCAGGGTTTGAAAATGTCTCTTGCTGTGTTCACAGCTTTCAGCATTGGTGTTGCGGTTCCTGTGTATGCAGTCATTTTCCAGCAAAAGAAGACAGCATCTGGCTGA
Protein sequence

>MsG0180002100.01.T01

MALNNGLRSAVKLIASSESSISKSVNRGFHSTGVKRMGGGHGHSEPEYMHAYHMYNLDKMKYQGLKMSLAVFTAFSIGVAVPVYAVIFQQKKTASG*