Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014644.01.T01 | KDO61249.1 | 51.128 | 133 | 28 | 2 | 4 | 99 | 64 | 196 | 1.98E-30 | 117 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014644.01.T01 | G7J263 | 60.150 | 133 | 16 | 1 | 4 | 99 | 127 | 259 | 6.19e-39 | 139 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000575.01 | MsG0380014644.01 | 0.806597 | 7.616121e-50 | 4.150503e-47 |
| MsG0180001886.01 | MsG0380014644.01 | 0.819508 | 1.119420e-52 | 8.631324e-50 |
| MsG0180004152.01 | MsG0380014644.01 | 0.805504 | 1.293173e-49 | 6.852958e-47 |
| MsG0180004481.01 | MsG0380014644.01 | 0.827647 | 1.393737e-54 | 1.353617e-51 |
| MsG0180004482.01 | MsG0380014644.01 | 0.806511 | 7.939736e-50 | 4.317475e-47 |
| MsG0180004509.01 | MsG0380014644.01 | 0.832245 | 1.055025e-55 | 1.172389e-52 |
| MsG0180004928.01 | MsG0380014644.01 | 0.813832 | 2.096792e-51 | 1.383952e-48 |
| MsG0380014644.01 | MsG0380016067.01 | 0.858752 | 6.639610e-63 | 1.712565e-59 |
| MsG0380014644.01 | MsG0380016860.01 | 0.842926 | 1.919064e-58 | 2.953983e-55 |
| MsG0380014644.01 | MsG0480021451.01 | 0.800240 | 1.581065e-48 | 7.324964e-46 |
| MsG0380014644.01 | MsG0480022223.01 | 0.801881 | 7.303521e-49 | 3.527403e-46 |
| MsG0380014644.01 | MsG0480022696.01 | 0.831095 | 2.026448e-55 | 2.176795e-52 |
| MsG0380014644.01 | MsG0480022711.01 | 0.815046 | 1.129597e-51 | 7.704831e-49 |
| MsG0380014644.01 | MsG0680031507.01 | 0.809704 | 1.659234e-50 | 9.810911e-48 |
| MsG0380014644.01 | MsG0780040545.01 | 0.818565 | 1.834265e-52 | 1.377808e-49 |
| MsG0380014644.01 | MsG0780041392.01 | 0.819495 | 1.127369e-52 | 8.689488e-50 |
| MsG0380014644.01 | MsG0780041462.01 | 0.823165 | 1.604208e-53 | 1.370681e-50 |
| MsG0380014644.01 | MsG0880045145.01 | 0.822892 | 1.857208e-53 | 1.574683e-50 |
| MsG0380014644.01 | MsG0880046631.01 | 0.830158 | 3.437303e-55 | 3.592350e-52 |
| MsG0380014644.01 | MsG0880047472.01 | 0.801410 | 9.122354e-49 | 4.353424e-46 |
| MsG0380014644.01 | MsG0880047764.01 | 0.803442 | 3.479611e-49 | 1.748744e-46 |
| MsG0280006593.01 | MsG0380014644.01 | 0.807938 | 3.958279e-50 | 2.233546e-47 |
| MsG0280006678.01 | MsG0380014644.01 | 0.838694 | 2.470029e-57 | 3.334307e-54 |
| MsG0280010233.01 | MsG0380014644.01 | 0.802821 | 4.677688e-49 | 2.313937e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014644.01.T01 | MTR_3g061590 | 60.150 | 133 | 16 | 1 | 4 | 99 | 127 | 259 | 1.57e-42 | 139 |
| MsG0380014644.01.T01 | MTR_8g073580 | 53.846 | 52 | 24 | 0 | 48 | 99 | 191 | 242 | 2.36e-11 | 58.2 |
| MsG0380014644.01.T01 | MTR_8g073580 | 53.846 | 52 | 24 | 0 | 48 | 99 | 238 | 289 | 3.92e-11 | 57.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380014644.01.T01 | AT3G20890 | 69.231 | 52 | 16 | 0 | 48 | 99 | 178 | 229 | 3.15e-19 | 79.0 |
| MsG0380014644.01.T01 | AT3G20890 | 69.231 | 52 | 16 | 0 | 48 | 99 | 241 | 292 | 2.49e-18 | 77.4 |
Find 26 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTCAGAGGGAGACCATC+TGG | 0.353842 | 3:-57669663 | MsG0380014644.01.T01:intron |
| TCCATCTCACCATGCGTTGA+AGG | 0.422533 | 3:+57669546 | None:intergenic |
| ACTACAAGCAATTGCAAATG+AGG | 0.452387 | 3:-57669875 | MsG0380014644.01.T01:CDS |
| CTCCATCATATGATTTAGCT+CGG | 0.453352 | 3:+57669812 | None:intergenic |
| TCCATCATATGATTTAGCTC+GGG | 0.508153 | 3:+57669813 | None:intergenic |
| CTCGGGGAGCACTACGACGA+GGG | 0.508597 | 3:+57669830 | None:intergenic |
| CCATCTCACCATGCGTTGAA+GGG | 0.532489 | 3:+57669547 | None:intergenic |
| CCCCGAGCTAAATCATATGA+TGG | 0.536873 | 3:-57669814 | MsG0380014644.01.T01:CDS |
| CCCTTCAACGCATGGTGAGA+TGG | 0.538750 | 3:-57669547 | MsG0380014644.01.T01:CDS |
| GCAAAGGATAGAATGACGCT+TGG | 0.544787 | 3:-57669591 | MsG0380014644.01.T01:CDS |
| CGTCATTCTATCCTTTGCCA+TGG | 0.545218 | 3:+57669596 | None:intergenic |
| CAAAGGATAGAATGACGCTT+GGG | 0.551160 | 3:-57669590 | MsG0380014644.01.T01:CDS |
| GAGCTATTCCCTTCAACGCA+TGG | 0.586026 | 3:-57669555 | MsG0380014644.01.T01:CDS |
| AATTCAGAGGGAGACCATCT+GGG | 0.590937 | 3:-57669662 | MsG0380014644.01.T01:intron |
| TGATGATTCAAAAGCAGCCA+TGG | 0.593984 | 3:-57669613 | MsG0380014644.01.T01:CDS |
| TTCAAAAGCAGCCATGGCAA+AGG | 0.597698 | 3:-57669607 | MsG0380014644.01.T01:CDS |
| ATGGAAGATGCAATTTCAAG+AGG | 0.609038 | 3:-57669528 | MsG0380014644.01.T01:CDS |
| CCATCATATGATTTAGCTCG+GGG | 0.614326 | 3:+57669814 | None:intergenic |
| TCACAATAAAGCCTCCCAGA+TGG | 0.627050 | 3:+57669648 | None:intergenic |
| TCGGGGAGCACTACGACGAG+GGG | 0.629479 | 3:+57669831 | None:intergenic |
| CGAGCTAAATCATATGATGG+AGG | 0.660288 | 3:-57669811 | MsG0380014644.01.T01:CDS |
| AAAAGATTCGCTGAGCACAC+AGG | 0.663588 | 3:-57669788 | MsG0380014644.01.T01:CDS |
| GCTCGGGGAGCACTACGACG+AGG | 0.665801 | 3:+57669829 | None:intergenic |
| AATGAGGTTTCAGATGCTCG+AGG | 0.692222 | 3:-57669859 | MsG0380014644.01.T01:CDS |
| GAGGTTTCAGATGCTCGAGG+CGG | 0.695093 | 3:-57669856 | MsG0380014644.01.T01:CDS |
| TCAGAGGGAGACCATCTGGG+AGG | 0.755162 | 3:-57669659 | MsG0380014644.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTACCATTTTCTGCTAATA+AGG | - | Chr3:57669681-57669700 | MsG0380014644.01.T01:intron | 25.0% |
| CACATTGTAATGAATTCAGA+GGG | - | Chr3:57669754-57669773 | MsG0380014644.01.T01:intron | 30.0% | |
| TCATCCTTATTAGCAGAAAA+TGG | + | Chr3:57669688-57669707 | None:intergenic | 30.0% | |
| TGCTAATAAGGATGACATAA+TGG | - | Chr3:57669693-57669712 | MsG0380014644.01.T01:intron | 30.0% | |
| ! | TTGAAGTTTTCAGAAGTAAG+AGG | - | Chr3:57669524-57669543 | MsG0380014644.01.T01:CDS | 30.0% |
| ACTACAAGCAATTGCAAATG+AGG | - | Chr3:57669553-57669572 | MsG0380014644.01.T01:CDS | 35.0% | |
| ATGGAAGATGCAATTTCAAG+AGG | - | Chr3:57669900-57669919 | MsG0380014644.01.T01:CDS | 35.0% | |
| CCACATTGTAATGAATTCAG+AGG | - | Chr3:57669753-57669772 | MsG0380014644.01.T01:intron | 35.0% | |
| CCTCTGAATTCATTACAATG+TGG | + | Chr3:57669756-57669775 | None:intergenic | 35.0% | |
| CTCCATCATATGATTTAGCT+CGG | + | Chr3:57669619-57669638 | None:intergenic | 35.0% | |
| ! | TCCATCATATGATTTAGCTC+GGG | + | Chr3:57669618-57669637 | None:intergenic | 35.0% |
| ! | TGACATAATGGAGTTCTTCA+AGG | - | Chr3:57669705-57669724 | MsG0380014644.01.T01:intron | 35.0% |
| CAAAGGATAGAATGACGCTT+GGG | - | Chr3:57669838-57669857 | MsG0380014644.01.T01:CDS | 40.0% | |
| CGAGCTAAATCATATGATGG+AGG | - | Chr3:57669617-57669636 | MsG0380014644.01.T01:CDS | 40.0% | |
| TGATGATTCAAAAGCAGCCA+TGG | - | Chr3:57669815-57669834 | MsG0380014644.01.T01:CDS | 40.0% | |
| ! | AGTTTTCAGAAGTAAGAGGC+AGG | - | Chr3:57669528-57669547 | MsG0380014644.01.T01:CDS | 40.0% |
| ! | CCATCATATGATTTAGCTCG+GGG | + | Chr3:57669617-57669636 | None:intergenic | 40.0% |
| ! | CTTCAAGGAGTATGTGTTGT+CGG | - | Chr3:57669720-57669739 | MsG0380014644.01.T01:intron | 40.0% |
| AAAAGATTCGCTGAGCACAC+AGG | - | Chr3:57669640-57669659 | MsG0380014644.01.T01:CDS | 45.0% | |
| AATGAGGTTTCAGATGCTCG+AGG | - | Chr3:57669569-57669588 | MsG0380014644.01.T01:CDS | 45.0% | |
| AATTCAGAGGGAGACCATCT+GGG | - | Chr3:57669766-57669785 | MsG0380014644.01.T01:intron | 45.0% | |
| CCCCGAGCTAAATCATATGA+TGG | - | Chr3:57669614-57669633 | MsG0380014644.01.T01:CDS | 45.0% | |
| CGTCATTCTATCCTTTGCCA+TGG | + | Chr3:57669835-57669854 | None:intergenic | 45.0% | |
| GCAAAGGATAGAATGACGCT+TGG | - | Chr3:57669837-57669856 | MsG0380014644.01.T01:CDS | 45.0% | |
| TCACAATAAAGCCTCCCAGA+TGG | + | Chr3:57669783-57669802 | None:intergenic | 45.0% | |
| TTCAAAAGCAGCCATGGCAA+AGG | - | Chr3:57669821-57669840 | MsG0380014644.01.T01:CDS | 45.0% | |
| CCATCTCACCATGCGTTGAA+GGG | + | Chr3:57669884-57669903 | None:intergenic | 50.0% | |
| GAATTCAGAGGGAGACCATC+TGG | - | Chr3:57669765-57669784 | MsG0380014644.01.T01:intron | 50.0% | |
| GAGCTATTCCCTTCAACGCA+TGG | - | Chr3:57669873-57669892 | MsG0380014644.01.T01:CDS | 50.0% | |
| TCCATCTCACCATGCGTTGA+AGG | + | Chr3:57669885-57669904 | None:intergenic | 50.0% | |
| ! | CAAGGAGTATGTGTTGTCGG+AGG | - | Chr3:57669723-57669742 | MsG0380014644.01.T01:intron | 50.0% |
| CCCTTCAACGCATGGTGAGA+TGG | - | Chr3:57669881-57669900 | MsG0380014644.01.T01:CDS | 55.0% | |
| GAGGTTTCAGATGCTCGAGG+CGG | - | Chr3:57669572-57669591 | MsG0380014644.01.T01:CDS | 55.0% | |
| !! | ACACAGGAGTGTTGCGACTG+AGG | - | Chr3:57669656-57669675 | MsG0380014644.01.T01:CDS | 55.0% |
| !! | ACAGGAGTGTTGCGACTGAG+GGG | - | Chr3:57669658-57669677 | MsG0380014644.01.T01:CDS | 55.0% |
| !! | CACAGGAGTGTTGCGACTGA+GGG | - | Chr3:57669657-57669676 | MsG0380014644.01.T01:CDS | 55.0% |
| TCAGAGGGAGACCATCTGGG+AGG | - | Chr3:57669769-57669788 | MsG0380014644.01.T01:intron | 60.0% | |
| !! | CTCGGGGAGCACTACGACGA+GGG | + | Chr3:57669601-57669620 | None:intergenic | 65.0% |
| !! | TCGGGGAGCACTACGACGAG+GGG | + | Chr3:57669600-57669619 | None:intergenic | 65.0% |
| !! | GCTCGGGGAGCACTACGACG+AGG | + | Chr3:57669602-57669621 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 57669521 | 57669929 | 57669521 | ID=MsG0380014644.01;Name=MsG0380014644.01 |
| Chr3 | mRNA | 57669521 | 57669929 | 57669521 | ID=MsG0380014644.01.T01;Parent=MsG0380014644.01;Name=MsG0380014644.01.T01;_AED=0.43;_eAED=0.46;_QI=0|0|0|1|1|1|2|0|99 |
| Chr3 | exon | 57669787 | 57669929 | 57669787 | ID=MsG0380014644.01.T01:exon:11176;Parent=MsG0380014644.01.T01 |
| Chr3 | exon | 57669521 | 57669677 | 57669521 | ID=MsG0380014644.01.T01:exon:11175;Parent=MsG0380014644.01.T01 |
| Chr3 | CDS | 57669787 | 57669929 | 57669787 | ID=MsG0380014644.01.T01:cds;Parent=MsG0380014644.01.T01 |
| Chr3 | CDS | 57669521 | 57669677 | 57669521 | ID=MsG0380014644.01.T01:cds;Parent=MsG0380014644.01.T01 |
| Gene Sequence |
| Protein sequence |
