AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780041392.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041392.01.T01 MTR_7g109980 95.833 120 5 0 1 120 1 120 5.49e-81 233
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0780041392.01.T01 AT4G01897 62.393 117 44 0 3 119 5 121 1.27e-54 167

Find 29 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTGATCTAGTTTGCTAAGA+TGG 0.325847 7:-90401108 None:intergenic
CAAAGAAATGGGTCCACTTT+CGG 0.360708 7:+90401055 MsG0780041392.01.T01:CDS
TCCTCAGCTTGGCAATGGTT+TGG 0.371213 7:+90401573 MsG0780041392.01.T01:intron
AAATAGTACAATATAATCAT+TGG 0.384411 7:-90401932 None:intergenic
TGGGAAGAATTGCAAAGAAA+TGG 0.392074 7:+90401043 MsG0780041392.01.T01:CDS
TCTTCAGATTGATGCTAAGA+AGG 0.429001 7:+90401299 MsG0780041392.01.T01:CDS
CCATCTTAGCAAACTAGATC+AGG 0.463319 7:+90401108 MsG0780041392.01.T01:CDS
CTATTTCCTCAGCTTGGCAA+TGG 0.465272 7:+90401568 MsG0780041392.01.T01:intron
GTCAAGTTCACCACCGAAAG+TGG 0.465358 7:-90401068 None:intergenic
GATGATCAAGGTTTGTCTGT+TGG 0.475434 7:-90401838 None:intergenic
TTTCTTGCGATGTGTGTGTA+AGG 0.481912 7:+90401787 MsG0780041392.01.T01:three_prime_UTR
AATCAGCACGGCGAAGGAAT+GGG 0.486850 7:+90401024 MsG0780041392.01.T01:CDS
GGGAAGAATTGCAAAGAAAT+GGG 0.488668 7:+90401044 MsG0780041392.01.T01:CDS
GAATCAGCACGGCGAAGGAA+TGG 0.494049 7:+90401023 MsG0780041392.01.T01:CDS
GAGAAGCTGTCTCTTTCAAA+TGG 0.500982 7:+90401691 MsG0780041392.01.T01:CDS
TCATTAAGAAAAGATGATCA+AGG 0.501413 7:-90401850 None:intergenic
AGCTGTCTCTTTCAAATGGT+CGG 0.507865 7:+90401695 MsG0780041392.01.T01:CDS
AGTTATTGACATGCTACTGT+TGG 0.527145 7:+90401889 MsG0780041392.01.T01:three_prime_UTR
TCTTAGCATCAATCTGAAGA+AGG 0.553114 7:-90401296 None:intergenic
TGGTTTGGTGTATGAAATTG+TGG 0.560108 7:+90401588 MsG0780041392.01.T01:CDS
GTACAGAATCAGCACGGCGA+AGG 0.581959 7:+90401018 MsG0780041392.01.T01:CDS
ATTGCAGGTTCGATCAACGT+TGG 0.585116 7:+90401239 MsG0780041392.01.T01:intron
TATCCCTCTCTCTCTAGATG+TGG 0.592426 7:+90401657 MsG0780041392.01.T01:CDS
GTGACCACATCTAGAGAGAG+AGG 0.596325 7:-90401661 None:intergenic
AGAGGGATAAAGCTTCGAGA+TGG 0.636294 7:-90401643 None:intergenic
TTGGTGTATGAAATTGTGGA+CGG 0.638694 7:+90401592 MsG0780041392.01.T01:CDS
AGAAATGGGTCCACTTTCGG+TGG 0.639639 7:+90401058 MsG0780041392.01.T01:CDS
TCTAGATGTGGTCACAATAG+CGG 0.643121 7:+90401669 MsG0780041392.01.T01:CDS
TGACCACATCTAGAGAGAGA+GGG 0.654116 7:-90401660 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAATTATTTTATTATTGC+AGG + Chr7:90401224-90401243 MsG0780041392.01.T01:intron 10.0%
!! AAATAGTACAATATAATCAT+TGG - Chr7:90401935-90401954 None:intergenic 15.0%
!! AAAAAAAAATGTAACTCTTG+TGG + Chr7:90402006-90402025 MsG0780041392.01.T01:three_prime_UTR 20.0%
!! AAAAAAAATGTAACTCTTGT+GGG + Chr7:90402007-90402026 MsG0780041392.01.T01:three_prime_UTR 20.0%
!! TTGATTTAACTAAAATCTGA+AGG + Chr7:90401386-90401405 MsG0780041392.01.T01:intron 20.0%
!!! ATTAAAAAAAGCATAGTGAA+AGG - Chr7:90401339-90401358 None:intergenic 20.0%
!!! GTTACATTTTTTTTTTTGAG+AGG - Chr7:90402001-90402020 None:intergenic 20.0%
!!! TTAAAAAAAGCATAGTGAAA+GGG - Chr7:90401338-90401357 None:intergenic 20.0%
! TCATTAAGAAAAGATGATCA+AGG - Chr7:90401853-90401872 None:intergenic 25.0%
!! TGATCATCTTTTCTTAATGA+AGG + Chr7:90401853-90401872 MsG0780041392.01.T01:three_prime_UTR 25.0%
TATAAGTCGCTTCATTCATT+CGG + Chr7:90401182-90401201 MsG0780041392.01.T01:intron 30.0%
!! AATAGCTTCCCTCATTTTTA+TGG + Chr7:90401619-90401638 MsG0780041392.01.T01:CDS 30.0%
!! ATAGCTTCCCTCATTTTTAT+GGG + Chr7:90401620-90401639 MsG0780041392.01.T01:CDS 30.0%
!!! CTGTTGGAATTTTAAGTTTC+TGG + Chr7:90401905-90401924 MsG0780041392.01.T01:three_prime_UTR 30.0%
AGTTATTGACATGCTACTGT+TGG + Chr7:90401889-90401908 MsG0780041392.01.T01:three_prime_UTR 35.0%
GGGAAGAATTGCAAAGAAAT+GGG + Chr7:90401044-90401063 MsG0780041392.01.T01:CDS 35.0%
TCTTAGCATCAATCTGAAGA+AGG - Chr7:90401299-90401318 None:intergenic 35.0%
TCTTCAGATTGATGCTAAGA+AGG + Chr7:90401299-90401318 MsG0780041392.01.T01:CDS 35.0%
TGGGAAGAATTGCAAAGAAA+TGG + Chr7:90401043-90401062 MsG0780041392.01.T01:CDS 35.0%
TTGGTGTATGAAATTGTGGA+CGG + Chr7:90401592-90401611 MsG0780041392.01.T01:CDS 35.0%
! TTTTGTGTACAGAATCAGCA+CGG + Chr7:90401012-90401031 MsG0780041392.01.T01:CDS 35.0%
!! TGGTTTGGTGTATGAAATTG+TGG + Chr7:90401588-90401607 MsG0780041392.01.T01:CDS 35.0%
AGCTGTCTCTTTCAAATGGT+CGG + Chr7:90401695-90401714 MsG0780041392.01.T01:CDS 40.0%
CAAAGAAATGGGTCCACTTT+CGG + Chr7:90401055-90401074 MsG0780041392.01.T01:CDS 40.0%
CCATCTTAGCAAACTAGATC+AGG + Chr7:90401108-90401127 MsG0780041392.01.T01:CDS 40.0%
GAGAAGCTGTCTCTTTCAAA+TGG + Chr7:90401691-90401710 MsG0780041392.01.T01:CDS 40.0%
GAGATGGCCCATAAAAATGA+GGG - Chr7:90401630-90401649 None:intergenic 40.0%
GATGATCAAGGTTTGTCTGT+TGG - Chr7:90401841-90401860 None:intergenic 40.0%
TCTAGATGTGGTCACAATAG+CGG + Chr7:90401669-90401688 MsG0780041392.01.T01:CDS 40.0%
TTTCTTGCGATGTGTGTGTA+AGG + Chr7:90401787-90401806 MsG0780041392.01.T01:three_prime_UTR 40.0%
!! CCTGATCTAGTTTGCTAAGA+TGG - Chr7:90401111-90401130 None:intergenic 40.0%
AGAGGGATAAAGCTTCGAGA+TGG - Chr7:90401646-90401665 None:intergenic 45.0%
ATTGCAGGTTCGATCAACGT+TGG + Chr7:90401239-90401258 MsG0780041392.01.T01:intron 45.0%
CGAGATGGCCCATAAAAATG+AGG - Chr7:90401631-90401650 None:intergenic 45.0%
CTATTTCCTCAGCTTGGCAA+TGG + Chr7:90401568-90401587 MsG0780041392.01.T01:intron 45.0%
TATCCCTCTCTCTCTAGATG+TGG + Chr7:90401657-90401676 MsG0780041392.01.T01:CDS 45.0%
TGACCACATCTAGAGAGAGA+GGG - Chr7:90401663-90401682 None:intergenic 45.0%
!! GCTATGCTATTTCCTCAGCT+TGG + Chr7:90401562-90401581 MsG0780041392.01.T01:intron 45.0%
AATCAGCACGGCGAAGGAAT+GGG + Chr7:90401024-90401043 MsG0780041392.01.T01:CDS 50.0%
ACCAAACCATTGCCAAGCTG+AGG - Chr7:90401577-90401596 None:intergenic 50.0%
AGAAATGGGTCCACTTTCGG+TGG + Chr7:90401058-90401077 MsG0780041392.01.T01:CDS 50.0%
GTCAAGTTCACCACCGAAAG+TGG - Chr7:90401071-90401090 None:intergenic 50.0%
GTGACCACATCTAGAGAGAG+AGG - Chr7:90401664-90401683 None:intergenic 50.0%
TCCTCAGCTTGGCAATGGTT+TGG + Chr7:90401573-90401592 MsG0780041392.01.T01:intron 50.0%
GAATCAGCACGGCGAAGGAA+TGG + Chr7:90401023-90401042 MsG0780041392.01.T01:CDS 55.0%
GTACAGAATCAGCACGGCGA+AGG + Chr7:90401018-90401037 MsG0780041392.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr7 gene 90400973 90402026 90400973 ID=MsG0780041392.01;Name=MsG0780041392.01
Chr7 mRNA 90400973 90402026 90400973 ID=MsG0780041392.01.T01;Parent=MsG0780041392.01;Name=MsG0780041392.01.T01;_AED=0.31;_eAED=0.31;_QI=31|1|1|1|1|1|3|285|120
Chr7 exon 90400973 90401129 90400973 ID=MsG0780041392.01.T01:exon:12339;Parent=MsG0780041392.01.T01
Chr7 exon 90401246 90401320 90401246 ID=MsG0780041392.01.T01:exon:12340;Parent=MsG0780041392.01.T01
Chr7 exon 90401580 90402026 90401580 ID=MsG0780041392.01.T01:exon:12341;Parent=MsG0780041392.01.T01
Chr7 five_prime_UTR 90400973 90401003 90400973 ID=MsG0780041392.01.T01:five_prime_utr;Parent=MsG0780041392.01.T01
Chr7 CDS 90401004 90401129 90401004 ID=MsG0780041392.01.T01:cds;Parent=MsG0780041392.01.T01
Chr7 CDS 90401246 90401320 90401246 ID=MsG0780041392.01.T01:cds;Parent=MsG0780041392.01.T01
Chr7 CDS 90401580 90401741 90401580 ID=MsG0780041392.01.T01:cds;Parent=MsG0780041392.01.T01
Chr7 three_prime_UTR 90401742 90402026 90401742 ID=MsG0780041392.01.T01:three_prime_utr;Parent=MsG0780041392.01.T01
Gene Sequence

>MsG0780041392.01.T01

ATGAGTGATTTTGTGTACAGAATCAGCACGGCGAAGGAATGGGAAGAATTGCAAAGAAATGGGTCCACTTTCGGTGGTGAACTTGACAAGTCTTCTTCTTTCATCCATCTTAGCAAACTAGATCAGGTTCGATCAACGTTGGATAATTTTTTCTTGAATTCTAAAGATGAACTCTACCTTCTTCAGATTGATGCTAAGAAGCTTGGCAATGGTTTGGTGTATGAAATTGTGGACGGTTCAAATAGCTTCCCTCATTTTTATGGGCCATCTCGAAGCTTTATCCCTCTCTCTCTAGATGTGGTCACAATAGCGGAGAAGCTGTCTCTTTCAAATGGTCGGTTCAGTTGTAGTTTGCTTGATTAA

Protein sequence

>MsG0780041392.01.T01

MSDFVYRISTAKEWEELQRNGSTFGGELDKSSSFIHLSKLDQVRSTLDNFFLNSKDELYLLQIDAKKLGNGLVYEIVDGSNSFPHFYGPSRSFIPLSLDVVTIAEKLSLSNGRFSCSLLD*