AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0880046631.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046631.01.T01 MTR_4g112780 75.949 158 20 4 1 145 1 153 5.10e-66 197
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0880046631.01.T01 AT5G08180 51.899 158 61 4 1 145 1 156 1.43e-47 151
MsG0880046631.01.T01 AT5G08180 51.899 158 61 4 1 145 1 156 1.43e-47 151

Find 25 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACAATGGATTAACATAT+TGG 0.284643 8:-75951170 MsG0880046631.01.T01:three_prime_UTR
CCCATTGCAAAACCTCTTGC+TGG 0.348880 8:-75953211 MsG0880046631.01.T01:CDS
TGAGATAGTTCACCCTTTGA+AGG 0.372203 8:+75951303 None:intergenic
CGCAGAGTGTGTATTATAGC+TGG 0.392404 8:-75952152 MsG0880046631.01.T01:intron
TAGTTCACCCTTTGAAGGCT+TGG 0.444404 8:+75951308 None:intergenic
ACATGAGTGATGACATCAAT+AGG 0.449731 8:+75952119 None:intergenic
TCCTTATCCTCACATAAAAT+CGG 0.452854 8:+75952095 None:intergenic
TTAGATGAGACATATACATA+TGG 0.460404 8:+75952068 None:intergenic
GTCCAGGACCTTGCAACGGC+AGG 0.468819 8:-75951368 MsG0880046631.01.T01:intron
CGACAGTGAAGGTGAGAAAT+CGG 0.483487 8:-75953275 MsG0880046631.01.T01:CDS
GTGATGACCAAGCCTTCAAA+GGG 0.500756 8:-75951315 MsG0880046631.01.T01:CDS
TCGTTGCTCCTGCCGTTGCA+AGG 0.501816 8:+75951360 None:intergenic
AGTGATGACCAAGCCTTCAA+AGG 0.525648 8:-75951316 MsG0880046631.01.T01:CDS
ACACTCAAGCTTGTTAAAAG+AGG 0.527156 8:-75953169 MsG0880046631.01.T01:CDS
CGGTGCAGAGGGAGAAGGAG+AGG 0.549279 8:-75953255 MsG0880046631.01.T01:CDS
GAAAGCTTCTTTCCAGCAAG+AGG 0.560382 8:+75953199 None:intergenic
GAAGGAGAGGAAGAAGATGC+AGG 0.571568 8:-75953242 MsG0880046631.01.T01:CDS
GAAATCGGTGCAGAGGGAGA+AGG 0.585599 8:-75953260 MsG0880046631.01.T01:CDS
AAGGTGAGAAATCGGTGCAG+AGG 0.589190 8:-75953267 MsG0880046631.01.T01:CDS
GCAGAGTGTGTATTATAGCT+GGG 0.592201 8:-75952151 MsG0880046631.01.T01:intron
AGGTGAGAAATCGGTGCAGA+GGG 0.594395 8:-75953266 MsG0880046631.01.T01:CDS
CATCACTAAAACACAACAAG+TGG 0.604554 8:+75951332 None:intergenic
AAAGGGTGAACTATCTCAAG+AGG 0.620179 8:-75951298 MsG0880046631.01.T01:CDS
ATTTGTCCAGGACCTTGCAA+CGG 0.636321 8:-75951372 MsG0880046631.01.T01:intron
GAAATGGGAAGCGACAGTGA+AGG 0.681973 8:-75953286 MsG0880046631.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAATTGAAACATAAAAAAT+GGG + Chr8:75952167-75952186 None:intergenic 15.0%
!! ATTCCAAAAAAAAGAAAAAA+CGG + Chr8:75951824-75951843 None:intergenic 15.0%
!! TTATAAGCTATAAACTATAT+TGG - Chr8:75951584-75951603 MsG0880046631.01.T01:intron 15.0%
!! ATAGCTTATAAAAACAACTT+AGG + Chr8:75951575-75951594 None:intergenic 20.0%
!! ATTCATAACATAACATTCTT+GGG + Chr8:75951736-75951755 None:intergenic 20.0%
!! CACAATTGAAACATAAAAAA+TGG + Chr8:75952168-75952187 None:intergenic 20.0%
!! CTATTGTATTCATAATATAC+AGG + Chr8:75952749-75952768 None:intergenic 20.0%
!! GAATTGTTGATATATTATGT+AGG + Chr8:75952820-75952839 None:intergenic 20.0%
!! TACATAAATCTTTGTCTTAT+GGG - Chr8:75951916-75951935 MsG0880046631.01.T01:intron 20.0%
!! TCATAAATAACTAAAAGTTG+AGG - Chr8:75952486-75952505 MsG0880046631.01.T01:intron 20.0%
!! TTACATAAATCTTTGTCTTA+TGG - Chr8:75951915-75951934 MsG0880046631.01.T01:intron 20.0%
!! TTCCATTCAAATATGTTATA+TGG + Chr8:75952610-75952629 None:intergenic 20.0%
!!! AAATGTATTTGTACTGTTTT+TGG - Chr8:75953188-75953207 MsG0880046631.01.T01:CDS 20.0%
!!! CATTTTTTATGTTTCAATTG+TGG - Chr8:75952166-75952185 MsG0880046631.01.T01:intron 20.0%
!!! GTTTTTTCTTTTTTTTGGAA+TGG - Chr8:75951823-75951842 MsG0880046631.01.T01:intron 20.0%
!!! TAATGAAAATCGTTAATTTG+TGG - Chr8:75951271-75951290 MsG0880046631.01.T01:CDS 20.0%
!!! TGTTGATTCTGTAATTAAAA+AGG - Chr8:75951858-75951877 MsG0880046631.01.T01:intron 20.0%
!!! TTTCAGTTTCGTTTTAAATA+TGG - Chr8:75952852-75952871 MsG0880046631.01.T01:intron 20.0%
! AATAAGTTGTTTATCCAAAC+AGG + Chr8:75951451-75951470 None:intergenic 25.0%
! ATATGGTATTAAGAGAAACA+TGG - Chr8:75952869-75952888 MsG0880046631.01.T01:intron 25.0%
! ATTTATCTTCCTTGACAAAA+TGG - Chr8:75952114-75952133 MsG0880046631.01.T01:CDS 25.0%
! GATTCATAACATAACATTCT+TGG + Chr8:75951737-75951756 None:intergenic 25.0%
! GTCCATATAACATATTTGAA+TGG - Chr8:75952605-75952624 MsG0880046631.01.T01:intron 25.0%
! TTAGATGAGACATATACATA+TGG + Chr8:75952315-75952334 None:intergenic 25.0%
!! CTTATTCTAAAGAGAGATTA+GGG + Chr8:75952412-75952431 None:intergenic 25.0%
!! TACATTTTGTAAGTCTGTTT+TGG + Chr8:75951675-75951694 None:intergenic 25.0%
!! TCTTATTCTAAAGAGAGATT+AGG + Chr8:75952413-75952432 None:intergenic 25.0%
!! TTTCAAAGTACTAGAGTAAT+TGG + Chr8:75952964-75952983 None:intergenic 25.0%
!!! AATTTTTGAGATATTTGTCC+AGG - Chr8:75952996-75953015 MsG0880046631.01.T01:intron 25.0%
!!! ATGAACTTGTTATGGAATTA+AGG - Chr8:75951424-75951443 MsG0880046631.01.T01:intron 25.0%
!!! CTTCAAAAATGAACTTGTTA+TGG - Chr8:75951416-75951435 MsG0880046631.01.T01:intron 25.0%
!!! TGAACTTGTTATGGAATTAA+GGG - Chr8:75951425-75951444 MsG0880046631.01.T01:intron 25.0%
AAATCTGCTATGCAATCATT+TGG - Chr8:75952914-75952933 MsG0880046631.01.T01:intron 30.0%
AACATTAGATTGCTCACAAA+GGG - Chr8:75952053-75952072 MsG0880046631.01.T01:intron 30.0%
ACAATTTGTGTTATGAATGC+CGG + Chr8:75952570-75952589 None:intergenic 30.0%
ATGGTAAAAAGACAGCAAAA+AGG + Chr8:75951789-75951808 None:intergenic 30.0%
GAGAAGAAGGCAAAAAATAA+GGG + Chr8:75952206-75952225 None:intergenic 30.0%
TAACATTAGATTGCTCACAA+AGG - Chr8:75952052-75952071 MsG0880046631.01.T01:intron 30.0%
TATGACATCAAATTAACCTC+AGG + Chr8:75952471-75952490 None:intergenic 30.0%
TCCTTATCCTCACATAAAAT+CGG + Chr8:75952288-75952307 None:intergenic 30.0%
TGAGAAGAAGGCAAAAAATA+AGG + Chr8:75952207-75952226 None:intergenic 30.0%
! ATAACACAAATTGTGTTGAC+AGG - Chr8:75952576-75952595 MsG0880046631.01.T01:intron 30.0%
! GAATGTATGCTTTTCTATAC+TGG - Chr8:75952627-75952646 MsG0880046631.01.T01:intron 30.0%
! GCTTGAATGTTTTGTCAATT+TGG - Chr8:75951891-75951910 MsG0880046631.01.T01:intron 30.0%
!! CTTGCAATCATTTTGATCTA+TGG - Chr8:75953241-75953260 MsG0880046631.01.T01:CDS 30.0%
!! GCTACAATGGATTAACATAT+TGG - Chr8:75953210-75953229 MsG0880046631.01.T01:CDS 30.0%
!!! CTGCCGTTTTTTCTTTTTTT+TGG - Chr8:75951818-75951837 MsG0880046631.01.T01:intron 30.0%
AAAACGGCAGCAAATTTGTA+TGG + Chr8:75951808-75951827 None:intergenic 35.0%
ACATGAGTGATGACATCAAT+AGG + Chr8:75952264-75952283 None:intergenic 35.0%
AGCACTCAAGTTACAAAACA+AGG + Chr8:75952537-75952556 None:intergenic 35.0%
CACAGTTTATCGAGAAAGAA+AGG - Chr8:75952775-75952794 MsG0880046631.01.T01:intron 35.0%
CATCACTAAAACACAACAAG+TGG + Chr8:75953051-75953070 None:intergenic 35.0%
CTTGACAAAATGGTTACTCA+AGG - Chr8:75952124-75952143 MsG0880046631.01.T01:CDS 35.0%
GAAAACAAGTGCATAAAGAG+AGG - Chr8:75951974-75951993 MsG0880046631.01.T01:intron 35.0%
GGAGAGTTCTTGAAAATAAG+TGG - Chr8:75951605-75951624 MsG0880046631.01.T01:intron 35.0%
TAAAGAGAGATTAGGGAAAC+AGG + Chr8:75952405-75952424 None:intergenic 35.0%
TAATATACAGGTCTATGAGC+TGG + Chr8:75952737-75952756 None:intergenic 35.0%
TCTATGAGCTGGATTATAGA+TGG + Chr8:75952726-75952745 None:intergenic 35.0%
TGAAATAAGCCAATCCAAAC+TGG - Chr8:75951710-75951729 MsG0880046631.01.T01:intron 35.0%
TTTCTTACACATATAGCCTG+AGG - Chr8:75952452-75952471 MsG0880046631.01.T01:intron 35.0%
! AACAAGCTTGAGTGTTTTCT+TGG + Chr8:75951206-75951225 None:intergenic 35.0%
! AGGAGGTGGTTAAAAGTATA+AGG - Chr8:75952002-75952021 MsG0880046631.01.T01:intron 35.0%
! CTTGAGTAACCATTTTGTCA+AGG + Chr8:75952126-75952145 None:intergenic 35.0%
! TCATGTTCCGATTTTATGTG+AGG - Chr8:75952278-75952297 MsG0880046631.01.T01:intron 35.0%
! TCCGATTTTATGTGAGGATA+AGG - Chr8:75952284-75952303 MsG0880046631.01.T01:intron 35.0%
!! ACACTCAAGCTTGTTAAAAG+AGG - Chr8:75951211-75951230 MsG0880046631.01.T01:exon 35.0%
!!! TGTACTGTTTTTGGCTACAA+TGG - Chr8:75953197-75953216 MsG0880046631.01.T01:CDS 35.0%
AAAGGGTGAACTATCTCAAG+AGG - Chr8:75953082-75953101 MsG0880046631.01.T01:intron 40.0%
AACTTTGCGCTGAATCTTGA+TGG - Chr8:75952349-75952368 MsG0880046631.01.T01:intron 40.0%
AAGGTGCAACATCATCAATG+AGG + Chr8:75952518-75952537 None:intergenic 40.0%
AGTATAAGGCGAGGTCAAAA+AGG - Chr8:75952016-75952035 MsG0880046631.01.T01:intron 40.0%
ATAACATTCTTGGGCCAGTT+TGG + Chr8:75951727-75951746 None:intergenic 40.0%
ATCAAGATTCAGCGCAAAGT+TGG + Chr8:75952350-75952369 None:intergenic 40.0%
CATAAAGAGAGGAGTTAAGG+AGG - Chr8:75951985-75952004 MsG0880046631.01.T01:intron 40.0%
GCAGAGTGTGTATTATAGCT+GGG - Chr8:75952229-75952248 MsG0880046631.01.T01:intron 40.0%
GCTTATTTCAGCTGATCTAC+TGG + Chr8:75951700-75951719 None:intergenic 40.0%
GTATAAGGCGAGGTCAAAAA+GGG - Chr8:75952017-75952036 MsG0880046631.01.T01:intron 40.0%
GTGCATAAAGAGAGGAGTTA+AGG - Chr8:75951982-75952001 MsG0880046631.01.T01:intron 40.0%
TATGGAATTAAGGGCCTGTT+TGG - Chr8:75951434-75951453 MsG0880046631.01.T01:intron 40.0%
TGAGATAGTTCACCCTTTGA+AGG + Chr8:75953080-75953099 None:intergenic 40.0%
! CTAAGCATTGCAGTGTTTCT+GGG - Chr8:75951297-75951316 MsG0880046631.01.T01:CDS 40.0%
! GTGGTTAAAAGTATAAGGCG+AGG - Chr8:75952007-75952026 MsG0880046631.01.T01:intron 40.0%
! TCTAAGCATTGCAGTGTTTC+TGG - Chr8:75951296-75951315 MsG0880046631.01.T01:CDS 40.0%
! TTGAGTGCTTTTCCTTATGC+CGG - Chr8:75952548-75952567 MsG0880046631.01.T01:intron 40.0%
!!! TTGCAGTGTTTCTGGGTTTT+TGG - Chr8:75951304-75951323 MsG0880046631.01.T01:CDS 40.0%
AAAGAGAGGAGTTAAGGAGG+TGG - Chr8:75951988-75952007 MsG0880046631.01.T01:intron 45.0%
AGAGAGATTAGGGAAACAGG+CGG + Chr8:75952402-75952421 None:intergenic 45.0%
AGTGATGACCAAGCCTTCAA+AGG - Chr8:75953064-75953083 MsG0880046631.01.T01:intron 45.0%
ATTCTTGGGCCAGTTTGGAT+TGG + Chr8:75951722-75951741 None:intergenic 45.0%
ATTTGTCCAGGACCTTGCAA+CGG - Chr8:75953008-75953027 MsG0880046631.01.T01:intron 45.0%
CGACAGTGAAGGTGAGAAAT+CGG - Chr8:75951105-75951124 MsG0880046631.01.T01:three_prime_UTR 45.0%
CGCAGAGTGTGTATTATAGC+TGG - Chr8:75952228-75952247 MsG0880046631.01.T01:intron 45.0%
GAAAGCTTCTTTCCAGCAAG+AGG + Chr8:75951184-75951203 None:intergenic 45.0%
GTGATGACCAAGCCTTCAAA+GGG - Chr8:75953065-75953084 MsG0880046631.01.T01:intron 45.0%
GTGTTATGAATGCCGGCATA+AGG + Chr8:75952563-75952582 None:intergenic 45.0%
! AGCAAGAGGTTTTGCAATGG+GGG + Chr8:75951170-75951189 None:intergenic 45.0%
! CAGCAAGAGGTTTTGCAATG+GGG + Chr8:75951171-75951190 None:intergenic 45.0%
! CCAGCAAGAGGTTTTGCAAT+GGG + Chr8:75951172-75951191 None:intergenic 45.0%
! TAGTTCACCCTTTGAAGGCT+TGG + Chr8:75953075-75953094 None:intergenic 45.0%
! TCCAGCAAGAGGTTTTGCAA+TGG + Chr8:75951173-75951192 None:intergenic 45.0%
AAGGTGAGAAATCGGTGCAG+AGG - Chr8:75951113-75951132 MsG0880046631.01.T01:three_prime_UTR 50.0%
AGGTGAGAAATCGGTGCAGA+GGG - Chr8:75951114-75951133 MsG0880046631.01.T01:three_prime_UTR 50.0%
CACACTCTGCGTTGAGAAGA+AGG + Chr8:75952219-75952238 None:intergenic 50.0%
CCCATTGCAAAACCTCTTGC+TGG - Chr8:75951169-75951188 MsG0880046631.01.T01:three_prime_UTR 50.0%
GAAGGAGAGGAAGAAGATGC+AGG - Chr8:75951138-75951157 MsG0880046631.01.T01:three_prime_UTR 50.0%
GAAATCGGTGCAGAGGGAGA+AGG - Chr8:75951120-75951139 MsG0880046631.01.T01:three_prime_UTR 55.0%
TCGTTGCTCCTGCCGTTGCA+AGG + Chr8:75953023-75953042 None:intergenic 60.0%
CGGTGCAGAGGGAGAAGGAG+AGG - Chr8:75951125-75951144 MsG0880046631.01.T01:three_prime_UTR 65.0%
CTCCTGCCGTTGCAAGGTCC+TGG + Chr8:75953017-75953036 None:intergenic 65.0%
GTCCAGGACCTTGCAACGGC+AGG - Chr8:75953012-75953031 MsG0880046631.01.T01:intron 65.0%
Chromosome Type Strat End Strand Name
Chr8 gene 75951097 75953305 75951097 ID=MsG0880046631.01;Name=MsG0880046631.01
Chr8 mRNA 75951097 75953305 75951097 ID=MsG0880046631.01.T01;Parent=MsG0880046631.01;Name=MsG0880046631.01.T01;_AED=0.50;_eAED=0.51;_QI=0|0|0.33|1|0|0|3|127|145
Chr8 exon 75951097 75951384 75951097 ID=MsG0880046631.01.T01:exon:32421;Parent=MsG0880046631.01.T01
Chr8 exon 75952060 75952169 75952060 ID=MsG0880046631.01.T01:exon:32420;Parent=MsG0880046631.01.T01
Chr8 exon 75953139 75953305 75953139 ID=MsG0880046631.01.T01:exon:32419;Parent=MsG0880046631.01.T01
Chr8 CDS 75953139 75953305 75953139 ID=MsG0880046631.01.T01:cds;Parent=MsG0880046631.01.T01
Chr8 CDS 75952060 75952169 75952060 ID=MsG0880046631.01.T01:cds;Parent=MsG0880046631.01.T01
Chr8 CDS 75951224 75951384 75951224 ID=MsG0880046631.01.T01:cds;Parent=MsG0880046631.01.T01
Chr8 three_prime_UTR 75951097 75951223 75951097 ID=MsG0880046631.01.T01:three_prime_utr;Parent=MsG0880046631.01.T01
Gene Sequence

>MsG0880046631.01.T01

ATGGGAAGCGACAGTGAAGGTGAGAAATCGGTGCAGAGGGAGAAGGAGAGGAAGAAGATGCAGGCTCTAGCCCCCATTGCAAAACCTCTTGCTGGAAAGAAGCTTTCCAAGAAAACACTCAAGCTTGTTAAAAGAGGTATTTTCGAAGCTTTTAAACACTATAGCTTAGTGTGTATTATAGCTGGGAACATATCGCCTATTGATGTCATCACTCATGTTCCGATTTTATGTGAGGATAAGGACATTCCATATGTATATGTCTCATCTAAAGAAGTAAGACCTTGCAACGGCAGGAGCAACGAAGAGACCACTTGTTGTGTTTTAGTGATGACCAAGCCTTCAAAGGGTGAACTATCTCAAGAGGTACAAGAGAAACTAAAGTCAGAGTATGATCAAGTTGCATCAGAGTGTAGAGAGCTTCAGTCTACACTTTTTTGA

Protein sequence

>MsG0880046631.01.T01

MGSDSEGEKSVQREKERKKMQALAPIAKPLAGKKLSKKTLKLVKRGIFEAFKHYSLVCIIAGNISPIDVITHVPILCEDKDIPYVYVSSKEVRPCNGRSNEETTCCVLVMTKPSKGELSQEVQEKLKSEYDQVASECRELQSTLF*