Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015933.01.T01 | XP_003601378.1 | 97.619 | 126 | 3 | 0 | 1 | 126 | 410 | 535 | 3.18E-79 | 253 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015933.01.T01 | Q8RW96 | 69.672 | 122 | 36 | 1 | 3 | 124 | 396 | 516 | 2.14E-53 | 178 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015933.01.T01 | G7J6P7 | 97.619 | 126 | 3 | 0 | 1 | 126 | 410 | 535 | 1.52e-79 | 253 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000470.01 | MsG0380015933.01 | 0.820919 | 5.318271e-53 | 4.264797e-50 |
| MsG0180005250.01 | MsG0380015933.01 | 0.805532 | 1.275815e-49 | 6.765816e-47 |
| MsG0180006261.01 | MsG0380015933.01 | 0.804055 | 2.595621e-49 | 1.325269e-46 |
| MsG0380011609.01 | MsG0380015933.01 | 0.822482 | 2.313293e-53 | 1.938721e-50 |
| MsG0380015566.01 | MsG0380015933.01 | 0.809682 | 1.676910e-50 | 9.910034e-48 |
| MsG0380015933.01 | MsG0380016910.01 | 0.805002 | 1.647108e-49 | 8.616949e-47 |
| MsG0380015933.01 | MsG0480019337.01 | 0.820830 | 5.575721e-53 | 4.460127e-50 |
| MsG0380015933.01 | MsG0480019795.01 | 0.806299 | 8.798657e-50 | 4.758777e-47 |
| MsG0380015933.01 | MsG0480021215.01 | 0.804097 | 2.544448e-49 | 1.300482e-46 |
| MsG0380015933.01 | MsG0480021536.01 | 0.803381 | 3.581545e-49 | 1.797125e-46 |
| MsG0380015933.01 | MsG0480021925.01 | 0.817492 | 3.206352e-52 | 2.338542e-49 |
| MsG0380015933.01 | MsG0480022795.01 | 0.805662 | 1.197972e-49 | 6.374547e-47 |
| MsG0380015933.01 | MsG0580024552.01 | 0.836744 | 7.819444e-57 | 9.945562e-54 |
| MsG0380015933.01 | MsG0580025135.01 | 0.800596 | 1.338263e-48 | 6.256280e-46 |
| MsG0380015933.01 | MsG0580025638.01 | 0.808498 | 3.007426e-50 | 1.722292e-47 |
| MsG0380015933.01 | MsG0580027922.01 | 0.817330 | 3.487882e-52 | 2.532531e-49 |
| MsG0380015933.01 | MsG0680030882.01 | 0.814672 | 1.367550e-51 | 9.234082e-49 |
| MsG0380015933.01 | MsG0680035692.01 | 0.810032 | 1.410226e-50 | 8.411061e-48 |
| MsG0380015933.01 | MsG0780037054.01 | 0.802753 | 4.829885e-49 | 2.385069e-46 |
| MsG0380015933.01 | MsG0880043937.01 | 0.809529 | 1.809154e-50 | 1.064785e-47 |
| MsG0280008112.01 | MsG0380015933.01 | 0.810825 | 9.509349e-51 | 5.791699e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015933.01.T01 | MTR_3g080070 | 97.619 | 126 | 3 | 0 | 1 | 126 | 410 | 535 | 3.86e-83 | 253 |
| MsG0380015933.01.T01 | MTR_3g086010 | 67.257 | 113 | 36 | 1 | 3 | 115 | 402 | 513 | 3.14e-45 | 154 |
| MsG0380015933.01.T01 | MTR_6g022720 | 60.714 | 112 | 43 | 1 | 3 | 114 | 396 | 506 | 3.96e-42 | 145 |
| MsG0380015933.01.T01 | MTR_3g107980 | 56.140 | 114 | 50 | 0 | 1 | 114 | 384 | 497 | 7.77e-36 | 128 |
| MsG0380015933.01.T01 | MTR_7g102920 | 57.843 | 102 | 43 | 0 | 4 | 105 | 22 | 123 | 1.84e-35 | 119 |
| MsG0380015933.01.T01 | MTR_1g011460 | 57.018 | 114 | 49 | 0 | 1 | 114 | 377 | 490 | 2.10e-35 | 127 |
| MsG0380015933.01.T01 | MTR_1g111570 | 55.357 | 112 | 49 | 1 | 3 | 114 | 378 | 488 | 4.32e-33 | 120 |
| MsG0380015933.01.T01 | MTR_1g062950 | 59.184 | 98 | 40 | 0 | 3 | 100 | 378 | 475 | 2.00e-32 | 119 |
| MsG0380015933.01.T01 | MTR_4g113020 | 43.443 | 122 | 69 | 0 | 4 | 125 | 387 | 508 | 1.46e-27 | 105 |
| MsG0380015933.01.T01 | MTR_1g112940 | 29.787 | 94 | 66 | 0 | 4 | 97 | 337 | 430 | 7.29e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0380015933.01.T01 | AT4G15415 | 69.672 | 122 | 36 | 1 | 3 | 124 | 396 | 516 | 2.18e-54 | 178 |
| MsG0380015933.01.T01 | AT4G15415 | 69.672 | 122 | 36 | 1 | 3 | 124 | 396 | 516 | 2.18e-54 | 178 |
| MsG0380015933.01.T01 | AT4G15415 | 69.672 | 122 | 36 | 1 | 3 | 124 | 396 | 516 | 2.18e-54 | 178 |
| MsG0380015933.01.T01 | AT4G15415 | 69.672 | 122 | 36 | 1 | 3 | 124 | 396 | 516 | 2.18e-54 | 178 |
| MsG0380015933.01.T01 | AT3G21650 | 66.102 | 118 | 40 | 0 | 3 | 120 | 417 | 534 | 2.08e-52 | 173 |
| MsG0380015933.01.T01 | AT3G21650 | 66.102 | 118 | 40 | 0 | 3 | 120 | 417 | 534 | 2.08e-52 | 173 |
| MsG0380015933.01.T01 | AT3G26020 | 67.619 | 105 | 34 | 0 | 1 | 105 | 406 | 510 | 3.81e-43 | 148 |
| MsG0380015933.01.T01 | AT3G26020 | 67.619 | 105 | 34 | 0 | 1 | 105 | 406 | 510 | 3.81e-43 | 148 |
| MsG0380015933.01.T01 | AT3G26020 | 67.619 | 105 | 34 | 0 | 1 | 105 | 406 | 510 | 3.81e-43 | 148 |
| MsG0380015933.01.T01 | AT3G26020 | 67.619 | 105 | 34 | 0 | 1 | 105 | 406 | 510 | 3.81e-43 | 148 |
| MsG0380015933.01.T01 | AT3G26020 | 67.961 | 103 | 33 | 0 | 3 | 105 | 410 | 512 | 1.41e-42 | 146 |
| MsG0380015933.01.T01 | AT3G26020 | 67.961 | 103 | 33 | 0 | 3 | 105 | 410 | 512 | 1.41e-42 | 146 |
| MsG0380015933.01.T01 | AT3G26020 | 67.961 | 103 | 33 | 0 | 3 | 105 | 410 | 512 | 1.41e-42 | 146 |
| MsG0380015933.01.T01 | AT3G26020 | 67.961 | 103 | 33 | 0 | 3 | 105 | 427 | 529 | 1.78e-42 | 146 |
| MsG0380015933.01.T01 | AT1G13460 | 68.000 | 100 | 32 | 0 | 1 | 100 | 385 | 484 | 8.09e-42 | 144 |
| MsG0380015933.01.T01 | AT1G13460 | 68.000 | 100 | 32 | 0 | 1 | 100 | 385 | 484 | 8.09e-42 | 144 |
| MsG0380015933.01.T01 | AT5G25510 | 53.782 | 119 | 55 | 0 | 3 | 121 | 381 | 499 | 1.46e-35 | 127 |
| MsG0380015933.01.T01 | AT3G26030 | 54.000 | 100 | 46 | 0 | 3 | 102 | 375 | 474 | 6.94e-35 | 125 |
| MsG0380015933.01.T01 | AT3G09880 | 52.336 | 107 | 51 | 0 | 3 | 109 | 386 | 492 | 8.82e-34 | 122 |
| MsG0380015933.01.T01 | AT3G54930 | 59.184 | 98 | 40 | 0 | 3 | 100 | 395 | 492 | 2.68e-32 | 119 |
| MsG0380015933.01.T01 | AT3G54930 | 59.184 | 98 | 40 | 0 | 3 | 100 | 395 | 492 | 2.68e-32 | 119 |
| MsG0380015933.01.T01 | AT5G03470 | 51.042 | 96 | 47 | 0 | 3 | 98 | 386 | 481 | 2.47e-30 | 113 |
Find 22 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTCCTTAATTTCATCTTCT+TGG | 0.194898 | 3:+76837253 | None:intergenic |
| ATTCCAAGAAGATGAAATTA+AGG | 0.317220 | 3:-76837256 | MsG0380015933.01.T01:CDS |
| CTGAACGGGCACTGTTTCTA+TGG | 0.327712 | 3:-76837461 | MsG0380015933.01.T01:CDS |
| GGAATCCATGTGGAAGAAAT+TGG | 0.329641 | 3:-76837211 | MsG0380015933.01.T01:CDS |
| GCCGATAATCTTCCCTGCTT+TGG | 0.336864 | 3:-76837388 | MsG0380015933.01.T01:CDS |
| TCCAAAGCAGGGAAGATTAT+CGG | 0.387741 | 3:+76837387 | None:intergenic |
| ATGGCTACAGGTAGCTGAAC+GGG | 0.461747 | 3:-76837475 | MsG0380015933.01.T01:CDS |
| ACGAGAGAAGCAAGAGAAGC+GGG | 0.492255 | 3:-76837232 | MsG0380015933.01.T01:CDS |
| ATCTTCCAATTTCTTCCACA+TGG | 0.518321 | 3:+76837206 | None:intergenic |
| GTGGACTTGATTATGGCTAC+AGG | 0.532610 | 3:-76837487 | None:intergenic |
| TCACATTCAGAGTCAAACTC+TGG | 0.532668 | 3:+76837331 | None:intergenic |
| AACTGCAGCAACGGCAAGAG+AGG | 0.543678 | 3:+76837125 | None:intergenic |
| GAGTCAAACTCTGGACAGCT+TGG | 0.546137 | 3:+76837340 | None:intergenic |
| ATGAAGCAGTTCTTGCCTCA+AGG | 0.551323 | 3:-76837155 | MsG0380015933.01.T01:CDS |
| AACGAGAGAAGCAAGAGAAG+CGG | 0.557982 | 3:-76837233 | MsG0380015933.01.T01:CDS |
| TCTGGACAGCTTGGTTCCAG+TGG | 0.564294 | 3:+76837349 | None:intergenic |
| TGCTTCATTGCTTATAGACG+TGG | 0.582925 | 3:+76837170 | None:intergenic |
| GTTTCATTGAACTGCAGCAA+CGG | 0.591067 | 3:+76837116 | None:intergenic |
| GTGGAAGAAATTGGAAGATG+TGG | 0.612850 | 3:-76837202 | MsG0380015933.01.T01:CDS |
| TATGGCTACAGGTAGCTGAA+CGG | 0.628011 | 3:-76837476 | MsG0380015933.01.T01:CDS |
| AAGAGAAGCGGGAATCCATG+TGG | 0.736377 | 3:-76837221 | MsG0380015933.01.T01:CDS |
| AAGAGAGGAAGCAAACCTTG+AGG | 0.738771 | 3:+76837140 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATTCCAAGAAGATGAAATTA+AGG | - | Chr3:76837340-76837359 | MsG0380015933.01.T01:CDS | 25.0% |
| ! | GAATGTGAGAAAAATATTCT+CGG | - | Chr3:76837280-76837299 | MsG0380015933.01.T01:CDS | 25.0% |
| GTTCCTTAATTTCATCTTCT+TGG | + | Chr3:76837346-76837365 | None:intergenic | 30.0% | |
| ATCTTCCAATTTCTTCCACA+TGG | + | Chr3:76837393-76837412 | None:intergenic | 35.0% | |
| TGTGAGAAAAATATTCTCGG+AGG | - | Chr3:76837283-76837302 | MsG0380015933.01.T01:CDS | 35.0% | |
| GGAATCCATGTGGAAGAAAT+TGG | - | Chr3:76837385-76837404 | MsG0380015933.01.T01:CDS | 40.0% | |
| GTGGAAGAAATTGGAAGATG+TGG | - | Chr3:76837394-76837413 | MsG0380015933.01.T01:CDS | 40.0% | |
| TCACATTCAGAGTCAAACTC+TGG | + | Chr3:76837268-76837287 | None:intergenic | 40.0% | |
| TCCAAAGCAGGGAAGATTAT+CGG | + | Chr3:76837212-76837231 | None:intergenic | 40.0% | |
| TGCTTCATTGCTTATAGACG+TGG | + | Chr3:76837429-76837448 | None:intergenic | 40.0% | |
| ! | GTGCATTTTTCTCCAAAGCA+GGG | + | Chr3:76837223-76837242 | None:intergenic | 40.0% |
| !! | CTGCTTTGGAGAAAAATGCA+CGG | - | Chr3:76837222-76837241 | MsG0380015933.01.T01:CDS | 40.0% |
| !! | TGCTTTGGAGAAAAATGCAC+GGG | - | Chr3:76837223-76837242 | MsG0380015933.01.T01:CDS | 40.0% |
| AACGAGAGAAGCAAGAGAAG+CGG | - | Chr3:76837363-76837382 | MsG0380015933.01.T01:CDS | 45.0% | |
| AAGAGAGGAAGCAAACCTTG+AGG | + | Chr3:76837459-76837478 | None:intergenic | 45.0% | |
| ATGAAGCAGTTCTTGCCTCA+AGG | - | Chr3:76837441-76837460 | MsG0380015933.01.T01:CDS | 45.0% | |
| TATGGCTACAGGTAGCTGAA+CGG | - | Chr3:76837120-76837139 | MsG0380015933.01.T01:CDS | 45.0% | |
| ! | CGTGCATTTTTCTCCAAAGC+AGG | + | Chr3:76837224-76837243 | None:intergenic | 45.0% |
| !! | GCTTTGGAGAAAAATGCACG+GGG | - | Chr3:76837224-76837243 | MsG0380015933.01.T01:CDS | 45.0% |
| AAGAGAAGCGGGAATCCATG+TGG | - | Chr3:76837375-76837394 | MsG0380015933.01.T01:CDS | 50.0% | |
| ACGAGAGAAGCAAGAGAAGC+GGG | - | Chr3:76837364-76837383 | MsG0380015933.01.T01:CDS | 50.0% | |
| ATGGCTACAGGTAGCTGAAC+GGG | - | Chr3:76837121-76837140 | MsG0380015933.01.T01:CDS | 50.0% | |
| GAGTCAAACTCTGGACAGCT+TGG | + | Chr3:76837259-76837278 | None:intergenic | 50.0% | |
| GCCGATAATCTTCCCTGCTT+TGG | - | Chr3:76837208-76837227 | MsG0380015933.01.T01:CDS | 50.0% | |
| !! | CTGAACGGGCACTGTTTCTA+TGG | - | Chr3:76837135-76837154 | MsG0380015933.01.T01:CDS | 50.0% |
| AACTGCAGCAACGGCAAGAG+AGG | + | Chr3:76837474-76837493 | None:intergenic | 55.0% | |
| AGAAAAATGCACGGGGCCAC+TGG | - | Chr3:76837231-76837250 | MsG0380015933.01.T01:CDS | 55.0% | |
| TCTGGACAGCTTGGTTCCAG+TGG | + | Chr3:76837250-76837269 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr3 | gene | 76837119 | 76837499 | 76837119 | ID=MsG0380015933.01;Name=MsG0380015933.01 |
| Chr3 | mRNA | 76837119 | 76837499 | 76837119 | ID=MsG0380015933.01.T01;Parent=MsG0380015933.01;Name=MsG0380015933.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|126 |
| Chr3 | exon | 76837119 | 76837499 | 76837119 | ID=MsG0380015933.01.T01:exon:24490;Parent=MsG0380015933.01.T01 |
| Chr3 | CDS | 76837119 | 76837499 | 76837119 | ID=MsG0380015933.01.T01:cds;Parent=MsG0380015933.01.T01 |
| Gene Sequence |
| Protein sequence |
