AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0480019337.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 13 sgRNAs with CRISPR-Local

Find 13 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGATAATGTTGCTGTTAT+AGG 0.203344 4:+18629770 MsG0480019337.01.T01:CDS
ATCCTGTTGTTGCATGTAAT+AGG 0.349513 4:+18629671 MsG0480019337.01.T01:CDS
ACATGCAACAACAGGATATC+TGG 0.351520 4:-18629665 None:intergenic
TCCTGTTGTTGCATGTAATA+GGG 0.398078 4:+18629672 MsG0480019337.01.T01:CDS
CTTCTTCTACCGTTTGATGA+AGG 0.424965 4:-18629842 None:intergenic
AGTCATTTCGATATTCTTGT+TGG 0.454214 4:-18629896 None:intergenic
TTCTTCTACCGTTTGATGAA+GGG 0.463767 4:-18629841 None:intergenic
TCCCTATTACATGCAACAAC+AGG 0.466654 4:-18629673 None:intergenic
TCAAACGGTAGAAGAAGAAA+TGG 0.486110 4:+18629848 MsG0480019337.01.T01:CDS
CTGTTAGACCCTTCATCAAA+CGG 0.513492 4:+18629833 MsG0480019337.01.T01:CDS
TCAACATTCCTATTTAACAG+AGG 0.637888 4:-18629752 None:intergenic
TGCAGCTGTAGAATCAGTCA+TGG 0.650502 4:-18629717 None:intergenic
AGGCTCAGAAATTCAGACAA+CGG 0.673554 4:+18629790 MsG0480019337.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
TCAACATTCCTATTTAACAG+AGG - Chr4:18629755-18629774 None:intergenic 30.0%
!! GTTGATAATGTTGCTGTTAT+AGG + Chr4:18629770-18629789 MsG0480019337.01.T01:CDS 30.0%
ATCCTGTTGTTGCATGTAAT+AGG + Chr4:18629671-18629690 MsG0480019337.01.T01:CDS 35.0%
TCAAACGGTAGAAGAAGAAA+TGG + Chr4:18629848-18629867 MsG0480019337.01.T01:CDS 35.0%
TCCTGTTGTTGCATGTAATA+GGG + Chr4:18629672-18629691 MsG0480019337.01.T01:CDS 35.0%
TTCTTCTACCGTTTGATGAA+GGG - Chr4:18629844-18629863 None:intergenic 35.0%
! CTTTTGTGCCTCTGTTAAAT+AGG + Chr4:18629744-18629763 MsG0480019337.01.T01:CDS 35.0%
ACATGCAACAACAGGATATC+TGG - Chr4:18629668-18629687 None:intergenic 40.0%
AGGCTCAGAAATTCAGACAA+CGG + Chr4:18629790-18629809 MsG0480019337.01.T01:CDS 40.0%
CTGTTAGACCCTTCATCAAA+CGG + Chr4:18629833-18629852 MsG0480019337.01.T01:CDS 40.0%
CTTCTTCTACCGTTTGATGA+AGG - Chr4:18629845-18629864 None:intergenic 40.0%
TCCCTATTACATGCAACAAC+AGG - Chr4:18629676-18629695 None:intergenic 40.0%
TGCAGCTGTAGAATCAGTCA+TGG - Chr4:18629720-18629739 None:intergenic 45.0%
Chromosome Type Strat End Strand Name
Chr4 gene 18629656 18629916 18629656 ID=MsG0480019337.01;Name=MsG0480019337.01
Chr4 mRNA 18629656 18629916 18629656 ID=MsG0480019337.01.T01;Parent=MsG0480019337.01;Name=MsG0480019337.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|86
Chr4 exon 18629656 18629916 18629656 ID=MsG0480019337.01.T01:exon:10874;Parent=MsG0480019337.01.T01
Chr4 CDS 18629656 18629916 18629656 ID=MsG0480019337.01.T01:cds;Parent=MsG0480019337.01.T01
Gene Sequence

>MsG0480019337.01.T01

ATGGCTTATCCAGATATCCTGTTGTTGCATGTAATAGGGAATCTTGCTCAAGATGTTGTTGCCATGACTGATTCTACAGCTGCAGCAGCTTTTGTGCCTCTGTTAAATAGGAATGTTGATAATGTTGCTGTTATAGGCTCAGAAATTCAGACAACGGATAGAAACTTACAGATTAGTCTGTTAGACCCTTCATCAAACGGTAGAAGAAGAAATGGCGTTTCAAGACAAGTTTCAAATGAACCAACAAGAATATCGAAATGA

Protein sequence

>MsG0480019337.01.T01

MAYPDILLLHVIGNLAQDVVAMTDSTAAAAFVPLLNRNVDNVAVIGSEIQTTDRNLQISLLDPSSNGRRRNGVSRQVSNEPTRISK*