Alfalfa Gene Editing Database
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0480019337.01.T01 | XP_004506453.1 | 75.41 | 61 | 14 | 1 | 17 | 76 | 380 | 440 | 2.14E-17 | 86.3 |
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|---|---|---|---|---|---|---|---|---|---|---|
MsG0480019337.01.T01 | A0A1S2YL64 | 75.410 | 61 | 14 | 1 | 17 | 76 | 380 | 440 | 1.02e-17 | 86.3 |
Gene ID | Type | Classification |
---|
Gene ID | Type | Classification |
---|
Co-expression Network
Gene1 | Gene2 | correlation coefficient | p_value | FDR |
---|---|---|---|---|
MsG0080047869.01 | MsG0480019337.01 | 0.823348 | 1.453534e-53 | 1.248285e-50 |
MsG0080047944.01 | MsG0480019337.01 | 0.813271 | 2.785597e-51 | 1.810774e-48 |
MsG0080047959.01 | MsG0480019337.01 | 0.802203 | 6.272712e-49 | 3.054215e-46 |
MsG0080048856.01 | MsG0480019337.01 | 0.801266 | 9.763947e-49 | 4.642759e-46 |
MsG0080048874.01 | MsG0480019337.01 | 0.802481 | 5.496874e-49 | 2.695557e-46 |
MsG0180000470.01 | MsG0480019337.01 | 0.830988 | 2.153540e-55 | 2.306026e-52 |
MsG0180000550.01 | MsG0480019337.01 | 0.807570 | 4.739930e-50 | 2.648997e-47 |
MsG0180000650.01 | MsG0480019337.01 | 0.804434 | 2.164139e-49 | 1.115705e-46 |
MsG0180001063.01 | MsG0480019337.01 | 0.810093 | 1.367861e-50 | 8.170964e-48 |
MsG0180001410.01 | MsG0480019337.01 | 0.806429 | 8.263432e-50 | 4.484129e-47 |
MsG0180001971.01 | MsG0480019337.01 | 0.812928 | 3.312658e-51 | 2.133499e-48 |
MsG0180002089.01 | MsG0480019337.01 | 0.818212 | 2.205353e-52 | 1.640489e-49 |
MsG0180002195.01 | MsG0480019337.01 | 0.810738 | 9.926996e-51 | 6.032168e-48 |
MsG0180003669.01 | MsG0480019337.01 | 0.840686 | 7.490139e-58 | 1.075142e-54 |
MsG0180004264.01 | MsG0480019337.01 | 0.829438 | 5.147256e-55 | 5.268053e-52 |
MsG0180005050.01 | MsG0480019337.01 | 0.802840 | 4.634465e-49 | 2.293696e-46 |
MsG0180005250.01 | MsG0480019337.01 | 0.801340 | 9.431769e-49 | 4.493129e-46 |
MsG0180005763.01 | MsG0480019337.01 | 0.806669 | 7.353691e-50 | 4.014765e-47 |
MsG0180006257.01 | MsG0480019337.01 | 0.828320 | 9.601245e-55 | 9.509906e-52 |
MsG0280006324.01 | MsG0480019337.01 | 0.816316 | 5.888817e-52 | 4.159044e-49 |
MsG0280010698.01 | MsG0480019337.01 | 0.811487 | 6.829793e-51 | 4.233333e-48 |
MsG0280011156.01 | MsG0480019337.01 | 0.800705 | 1.271729e-48 | 5.961407e-46 |
MsG0280011175.01 | MsG0480019337.01 | 0.816875 | 4.413020e-52 | 3.164808e-49 |
MsG0380011609.01 | MsG0480019337.01 | 0.826872 | 2.137431e-54 | 2.029564e-51 |
MsG0380011755.01 | MsG0480019337.01 | 0.803295 | 3.732536e-49 | 1.868732e-46 |
MsG0380011895.01 | MsG0480019337.01 | 0.801012 | 1.100555e-48 | 5.200000e-46 |
MsG0380014251.01 | MsG0480019337.01 | 0.811998 | 5.289183e-51 | 3.323065e-48 |
MsG0380014537.01 | MsG0480019337.01 | 0.800596 | 1.337949e-48 | 6.254877e-46 |
MsG0380014573.01 | MsG0480019337.01 | 0.803413 | 3.528041e-49 | 1.771780e-46 |
MsG0480018964.01 | MsG0480019337.01 | 0.829586 | 4.738980e-55 | 4.871198e-52 |
MsG0480019336.01 | MsG0480019337.01 | 0.805837 | 1.100724e-49 | 5.883596e-47 |
MsG0480019337.01 | MsG0480019795.01 | 0.811835 | 5.738232e-51 | 3.589391e-48 |
MsG0480019337.01 | MsG0480020634.01 | 0.818541 | 1.857459e-52 | 1.394366e-49 |
MsG0480019337.01 | MsG0480021536.01 | 0.820877 | 5.438469e-53 | 4.356144e-50 |
MsG0480019337.01 | MsG0480021558.01 | 0.814751 | 1.313293e-51 | 8.886671e-49 |
MsG0480019337.01 | MsG0480021853.01 | 0.818127 | 2.305160e-52 | 1.710752e-49 |
MsG0480019337.01 | MsG0480021925.01 | 0.813357 | 2.666262e-51 | 1.737397e-48 |
MsG0480019337.01 | MsG0480022222.01 | 0.800004 | 1.766458e-48 | 8.134860e-46 |
MsG0480019337.01 | MsG0480022259.01 | 0.816658 | 4.935646e-52 | 3.518572e-49 |
MsG0480019337.01 | MsG0480022795.01 | 0.801543 | 8.568501e-49 | 4.102844e-46 |
MsG0480019337.01 | MsG0480023654.01 | 0.809644 | 1.708957e-50 | 1.008949e-47 |
MsG0480019337.01 | MsG0480023707.01 | 0.810543 | 1.093947e-50 | 6.612380e-48 |
MsG0480019337.01 | MsG0580024552.01 | 0.811828 | 5.758200e-51 | 3.601216e-48 |
MsG0480019337.01 | MsG0580025022.01 | 0.823490 | 1.346884e-53 | 1.161450e-50 |
MsG0480019337.01 | MsG0580025201.01 | 0.800530 | 1.380307e-48 | 6.442045e-46 |
MsG0480019337.01 | MsG0580025638.01 | 0.807278 | 5.465386e-50 | 3.031586e-47 |
MsG0480019337.01 | MsG0580026165.01 | 0.802140 | 6.461459e-49 | 3.141149e-46 |
MsG0480019337.01 | MsG0580027833.01 | 0.807112 | 5.925987e-50 | 3.272687e-47 |
MsG0480019337.01 | MsG0580027922.01 | 0.803496 | 3.391322e-49 | 1.706773e-46 |
MsG0480019337.01 | MsG0580028527.01 | 0.800526 | 1.383222e-48 | 6.454899e-46 |
MsG0480019337.01 | MsG0580028990.01 | 0.808472 | 3.046275e-50 | 1.743377e-47 |
MsG0480019337.01 | MsG0580029554.01 | 0.802165 | 6.385574e-49 | 3.106291e-46 |
MsG0480019337.01 | MsG0680030316.01 | 0.813625 | 2.328258e-51 | 1.528209e-48 |
MsG0480019337.01 | MsG0680030882.01 | 0.823258 | 1.526133e-53 | 1.307351e-50 |
MsG0480019337.01 | MsG0680031355.01 | 0.815852 | 7.475393e-52 | 5.212413e-49 |
MsG0480019337.01 | MsG0680031505.01 | 0.807076 | 6.032158e-50 | 3.328204e-47 |
MsG0480019337.01 | MsG0680032996.01 | 0.806373 | 8.490895e-50 | 4.600907e-47 |
MsG0480019337.01 | MsG0680033412.01 | 0.815476 | 9.066551e-52 | 6.257967e-49 |
MsG0480019337.01 | MsG0680034230.01 | 0.806382 | 8.451085e-50 | 4.580402e-47 |
MsG0480019337.01 | MsG0680034565.01 | 0.823058 | 1.699390e-53 | 1.447695e-50 |
MsG0480019337.01 | MsG0680035347.01 | 0.816295 | 5.951540e-52 | 4.200991e-49 |
MsG0480019337.01 | MsG0680035573.01 | 0.813521 | 2.454674e-51 | 1.606643e-48 |
MsG0480019337.01 | MsG0680035692.01 | 0.841580 | 4.358324e-58 | 6.432506e-55 |
MsG0480019337.01 | MsG0680035694.01 | 0.804457 | 2.140048e-49 | 1.103960e-46 |
MsG0480019337.01 | MsG0680035844.01 | 0.830393 | 3.012208e-55 | 3.169504e-52 |
MsG0480019337.01 | MsG0780036128.01 | 0.826430 | 2.724309e-54 | 2.554435e-51 |
MsG0480019337.01 | MsG0780036498.01 | 0.808488 | 3.022010e-50 | 1.730185e-47 |
MsG0480019337.01 | MsG0780037054.01 | 0.813435 | 2.563555e-51 | 1.674005e-48 |
MsG0480019337.01 | MsG0780038391.01 | 0.806799 | 6.901729e-50 | 3.780877e-47 |
MsG0480019337.01 | MsG0780038401.01 | 0.810226 | 1.280531e-50 | 7.675531e-48 |
MsG0480019337.01 | MsG0780038654.01 | 0.805498 | 1.296493e-49 | 6.869706e-47 |
MsG0480019337.01 | MsG0780039252.01 | 0.806616 | 7.543017e-50 | 4.112688e-47 |
MsG0480019337.01 | MsG0780040576.01 | -0.806401 | 8.375264e-50 | 4.541472e-47 |
MsG0480019337.01 | MsG0780040596.01 | 0.820539 | 6.501281e-53 | 5.158600e-50 |
MsG0480019337.01 | MsG0780040704.01 | 0.810836 | 9.454844e-51 | 5.760358e-48 |
MsG0480019337.01 | MsG0780041456.01 | 0.840165 | 1.024505e-57 | 1.447221e-54 |
MsG0480019337.01 | MsG0880041836.01 | 0.838974 | 2.090730e-57 | 2.846446e-54 |
MsG0480019337.01 | MsG0880042232.01 | 0.800776 | 1.229517e-48 | 5.774145e-46 |
MsG0480019337.01 | MsG0880042294.01 | 0.812462 | 4.187919e-51 | 2.664644e-48 |
MsG0480019337.01 | MsG0880042698.01 | 0.802605 | 5.181401e-49 | 2.548936e-46 |
MsG0480019337.01 | MsG0880042800.01 | 0.817770 | 2.775726e-52 | 2.039983e-49 |
MsG0480019337.01 | MsG0880042966.01 | 0.832852 | 7.461429e-56 | 8.443207e-53 |
MsG0480019337.01 | MsG0880043509.01 | 0.807602 | 4.665922e-50 | 2.609794e-47 |
MsG0480019337.01 | MsG0880044085.01 | 0.805326 | 1.408759e-49 | 7.431753e-47 |
MsG0480019337.01 | MsG0880044415.01 | 0.801513 | 8.691674e-49 | 4.158653e-46 |
MsG0480019337.01 | MsG0880044416.01 | 0.806419 | 8.301230e-50 | 4.503454e-47 |
MsG0480019337.01 | MsG0880044574.01 | 0.820516 | 6.580439e-53 | 5.217811e-50 |
MsG0480019337.01 | MsG0880045895.01 | 0.800497 | 1.401661e-48 | 6.536300e-46 |
MsG0480019337.01 | MsG0880045896.01 | 0.813066 | 3.088818e-51 | 1.996757e-48 |
MsG0480019337.01 | MsG0880045937.01 | 0.810514 | 1.110177e-50 | 6.705346e-48 |
MsG0480019337.01 | MsG0880046620.01 | 0.803193 | 3.918320e-49 | 1.956721e-46 |
MsG0380015566.01 | MsG0480019337.01 | 0.834308 | 3.230471e-56 | 3.818969e-53 |
MsG0380015720.01 | MsG0480019337.01 | 0.832189 | 1.089440e-55 | 1.208691e-52 |
MsG0380015933.01 | MsG0480019337.01 | 0.820830 | 5.575721e-53 | 4.460127e-50 |
MsG0380016004.01 | MsG0480019337.01 | 0.804617 | 1.982209e-49 | 1.026751e-46 |
MsG0380016064.01 | MsG0480019337.01 | 0.819615 | 1.058297e-52 | 8.183785e-50 |
MsG0380016475.01 | MsG0480019337.01 | 0.822125 | 2.800182e-53 | 2.322884e-50 |
MsG0380016910.01 | MsG0480019337.01 | 0.822412 | 2.402560e-53 | 2.009550e-50 |
MsG0380017140.01 | MsG0480019337.01 | 0.811030 | 8.582981e-51 | 5.256373e-48 |
MsG0380017461.01 | MsG0480019337.01 | 0.827442 | 1.560640e-54 | 1.506949e-51 |
MsG0380017680.01 | MsG0480019337.01 | 0.819364 | 1.207223e-52 | 9.270061e-50 |
MsG0480018163.01 | MsG0480019337.01 | 0.821273 | 4.408608e-53 | 3.569991e-50 |
MsG0480018250.01 | MsG0480019337.01 | 0.800427 | 1.448345e-48 | 6.742299e-46 |
MsG0280006491.01 | MsG0480019337.01 | 0.807189 | 5.706460e-50 | 3.157942e-47 |
MsG0280008112.01 | MsG0480019337.01 | 0.811421 | 7.059531e-51 | 4.368018e-48 |
MsG0280008615.01 | MsG0480019337.01 | 0.839160 | 1.870349e-57 | 2.560879e-54 |
MsG0280008894.01 | MsG0480019337.01 | 0.821920 | 3.123879e-53 | 2.576307e-50 |
MsG0280009297.01 | MsG0480019337.01 | 0.807544 | 4.800094e-50 | 2.680903e-47 |
MsG0280010443.01 | MsG0480019337.01 | 0.823580 | 1.282853e-53 | 1.109045e-50 |
PPI
Gene1 | Gene2 | Type |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
---|
Find 13 sgRNAs with CRISPR-Local
Find 13 sgRNAs with CRISPR-GE
CRISPR-Local
sgRNA_sequence | on_target_score | Position | Region |
---|---|---|---|
GTTGATAATGTTGCTGTTAT+AGG | 0.203344 | 4:+18629770 | MsG0480019337.01.T01:CDS |
ATCCTGTTGTTGCATGTAAT+AGG | 0.349513 | 4:+18629671 | MsG0480019337.01.T01:CDS |
ACATGCAACAACAGGATATC+TGG | 0.351520 | 4:-18629665 | None:intergenic |
TCCTGTTGTTGCATGTAATA+GGG | 0.398078 | 4:+18629672 | MsG0480019337.01.T01:CDS |
CTTCTTCTACCGTTTGATGA+AGG | 0.424965 | 4:-18629842 | None:intergenic |
AGTCATTTCGATATTCTTGT+TGG | 0.454214 | 4:-18629896 | None:intergenic |
TTCTTCTACCGTTTGATGAA+GGG | 0.463767 | 4:-18629841 | None:intergenic |
TCCCTATTACATGCAACAAC+AGG | 0.466654 | 4:-18629673 | None:intergenic |
TCAAACGGTAGAAGAAGAAA+TGG | 0.486110 | 4:+18629848 | MsG0480019337.01.T01:CDS |
CTGTTAGACCCTTCATCAAA+CGG | 0.513492 | 4:+18629833 | MsG0480019337.01.T01:CDS |
TCAACATTCCTATTTAACAG+AGG | 0.637888 | 4:-18629752 | None:intergenic |
TGCAGCTGTAGAATCAGTCA+TGG | 0.650502 | 4:-18629717 | None:intergenic |
AGGCTCAGAAATTCAGACAA+CGG | 0.673554 | 4:+18629790 | MsG0480019337.01.T01:CDS |
CRISPR-GE
badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
---|---|---|---|---|---|
TCAACATTCCTATTTAACAG+AGG | - | Chr4:18629755-18629774 | None:intergenic | 30.0% | |
!! | GTTGATAATGTTGCTGTTAT+AGG | + | Chr4:18629770-18629789 | MsG0480019337.01.T01:CDS | 30.0% |
ATCCTGTTGTTGCATGTAAT+AGG | + | Chr4:18629671-18629690 | MsG0480019337.01.T01:CDS | 35.0% | |
TCAAACGGTAGAAGAAGAAA+TGG | + | Chr4:18629848-18629867 | MsG0480019337.01.T01:CDS | 35.0% | |
TCCTGTTGTTGCATGTAATA+GGG | + | Chr4:18629672-18629691 | MsG0480019337.01.T01:CDS | 35.0% | |
TTCTTCTACCGTTTGATGAA+GGG | - | Chr4:18629844-18629863 | None:intergenic | 35.0% | |
! | CTTTTGTGCCTCTGTTAAAT+AGG | + | Chr4:18629744-18629763 | MsG0480019337.01.T01:CDS | 35.0% |
ACATGCAACAACAGGATATC+TGG | - | Chr4:18629668-18629687 | None:intergenic | 40.0% | |
AGGCTCAGAAATTCAGACAA+CGG | + | Chr4:18629790-18629809 | MsG0480019337.01.T01:CDS | 40.0% | |
CTGTTAGACCCTTCATCAAA+CGG | + | Chr4:18629833-18629852 | MsG0480019337.01.T01:CDS | 40.0% | |
CTTCTTCTACCGTTTGATGA+AGG | - | Chr4:18629845-18629864 | None:intergenic | 40.0% | |
TCCCTATTACATGCAACAAC+AGG | - | Chr4:18629676-18629695 | None:intergenic | 40.0% | |
TGCAGCTGTAGAATCAGTCA+TGG | - | Chr4:18629720-18629739 | None:intergenic | 45.0% |
Chromosome | Type | Strat | End | Strand | Name |
---|---|---|---|---|---|
Chr4 | gene | 18629656 | 18629916 | 18629656 | ID=MsG0480019337.01;Name=MsG0480019337.01 |
Chr4 | mRNA | 18629656 | 18629916 | 18629656 | ID=MsG0480019337.01.T01;Parent=MsG0480019337.01;Name=MsG0480019337.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|86 |
Chr4 | exon | 18629656 | 18629916 | 18629656 | ID=MsG0480019337.01.T01:exon:10874;Parent=MsG0480019337.01.T01 |
Chr4 | CDS | 18629656 | 18629916 | 18629656 | ID=MsG0480019337.01.T01:cds;Parent=MsG0480019337.01.T01 |
Gene Sequence |
Protein sequence |