AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0580024552.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0580024552.01.T01 MTR_5g013080 78.409 176 31 2 7 180 34 204 3.80e-90 262
MsG0580024552.01.T01 MTR_5g013140 69.608 102 31 0 7 108 147 248 8.28e-38 134
MsG0580024552.01.T01 MTR_5g013140 83.099 71 12 0 110 180 323 393 9.28e-35 126
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 40 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATATTTGACTGTTGAAAAT+TGG 0.316067 5:+6286106 None:intergenic
TCAAATATCACGGAGATTAC+AGG 0.343830 5:-6286092 MsG0580024552.01.T01:CDS
TGCAATGAGACCTACAAAAC+TGG 0.420267 5:-6286414 MsG0580024552.01.T01:intron
GCAATCATGGAAGTCCTGTT+TGG 0.422527 5:-6286478 MsG0580024552.01.T01:CDS
TATTGACAATACTTTAAATC+CGG 0.428551 5:-6286582 MsG0580024552.01.T01:CDS
TTGTAGTGTTCAACAAGTAT+AGG 0.428975 5:-6286191 MsG0580024552.01.T01:intron
TTGTGTTGGACACAAACTTT+CGG 0.442750 5:+6287061 None:intergenic
GTTCACTGTCTTCTTATACT+TGG 0.447231 5:+6286502 None:intergenic
AGAAGCATGCTTAACATCAT+TGG 0.458517 5:-6286450 MsG0580024552.01.T01:CDS
TCACGGAGATTACAGGGAAA+TGG 0.459332 5:-6286085 MsG0580024552.01.T01:CDS
GCAGCCATACATGCAACATA+AGG 0.463012 5:-6286549 MsG0580024552.01.T01:CDS
GCTTAAGAGATGGGTTCATA+AGG 0.466191 5:-6286009 MsG0580024552.01.T01:CDS
AAGACAGTGAACTGCAATCA+TGG 0.467236 5:-6286491 MsG0580024552.01.T01:CDS
TTCAAAGCAGGCCTACGGTC+AGG 0.467928 5:+6286041 None:intergenic
CATGCTTCTCCATTCCAAAC+AGG 0.474983 5:+6286464 None:intergenic
AAGAGATGGGTTCATAAGGT+TGG 0.479955 5:-6286005 MsG0580024552.01.T01:CDS
TGGCAGGGCGATGGTAGTGC+TGG 0.481700 5:-6286623 MsG0580024552.01.T01:intron
AGTGCCTTATGTTGCATGTA+TGG 0.483200 5:+6286545 None:intergenic
ACTGTCTTCTTATACTTGGT+CGG 0.489810 5:+6286506 None:intergenic
CGGTCAGGTTTACGTTTCTG+TGG 0.490934 5:+6286056 None:intergenic
AGCCTAGGTTGCTTCAAAGC+AGG 0.506799 5:+6286029 None:intergenic
AGCAACCTAGGCTTAAGAGA+TGG 0.510316 5:-6286019 MsG0580024552.01.T01:CDS
TCGGTTGCACATTCTCGAAC+CGG 0.514678 5:+6287080 None:intergenic
GAATGGTGTTGCAAAGAGAA+AGG 0.528695 5:-6286144 MsG0580024552.01.T01:CDS
GCAACCTAGGCTTAAGAGAT+GGG 0.542462 5:-6286018 MsG0580024552.01.T01:CDS
CAAATATCACGGAGATTACA+GGG 0.544371 5:-6286091 MsG0580024552.01.T01:CDS
CATGGAAGTCCTGTTTGGAA+TGG 0.547429 5:-6286473 MsG0580024552.01.T01:CDS
GGTCAGGTTTACGTTTCTGT+GGG 0.548383 5:+6286057 None:intergenic
GTGGAGGTAAATAAAGATGA+AGG 0.554468 5:-6287103 MsG0580024552.01.T01:CDS
TATCAAAAGAGCTATTGTGT+TGG 0.555066 5:+6287047 None:intergenic
TGAACCCATCTCTTAAGCCT+AGG 0.559582 5:+6286014 None:intergenic
AAGTATAGGAACAGTTCCAT+TGG 0.591926 5:-6286177 MsG0580024552.01.T01:CDS
GTTGCTTCAAAGCAGGCCTA+CGG 0.594096 5:+6286036 None:intergenic
GGTGGACAATGGCAGGGCGA+TGG 0.604468 5:-6286632 MsG0580024552.01.T01:intron
TTGCATAATGAGGGAAACAG+TGG 0.626434 5:-6287122 None:intergenic
GGCCTGCTTTGAAGCAACCT+AGG 0.627807 5:-6286031 MsG0580024552.01.T01:CDS
CATAATGAGGGAAACAGTGG+AGG 0.636407 5:-6287119 None:intergenic
AGGTAAATAAAGATGAAGGC+CGG 0.649787 5:-6287099 MsG0580024552.01.T01:CDS
CCATTGGCATGTGAAAAGAA+TGG 0.681612 5:-6286161 MsG0580024552.01.T01:CDS
AGAAACGTAAACCTGACCGT+AGG 0.705065 5:-6286052 MsG0580024552.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTGATTAGTAATTTGAAT+TGG - Chr5:6286140-6286159 MsG0580024552.01.T01:CDS 15.0%
!! ACAAGAAATAAAGAAAATGA+TGG - Chr5:6286839-6286858 MsG0580024552.01.T01:intron 20.0%
!! TATTGACAATACTTTAAATC+CGG - Chr5:6286522-6286541 MsG0580024552.01.T01:CDS 20.0%
!!! TAACTGAATGATCTATTTTT+AGG + Chr5:6286331-6286350 None:intergenic 20.0%
! AAGATGCATAATCAAAATTC+AGG + Chr5:6286181-6286200 None:intergenic 25.0%
! GATATTTGACTGTTGAAAAT+TGG + Chr5:6287001-6287020 None:intergenic 25.0%
! GATTATGCATCTTAGTAATA+TGG - Chr5:6286188-6286207 MsG0580024552.01.T01:CDS 25.0%
! TGAATGGAAATTGTTTAGTT+TGG - Chr5:6286268-6286287 MsG0580024552.01.T01:intron 25.0%
!! TAGCTCTTTTGATAAAAACA+TGG - Chr5:6286066-6286085 MsG0580024552.01.T01:CDS 25.0%
!! TTTTCAACAGTCAAATATCA+CGG - Chr5:6287002-6287021 MsG0580024552.01.T01:intron 25.0%
GAGATATTACAAGAGAAATC+AGG + Chr5:6286108-6286127 None:intergenic 30.0%
TATCAAAAGAGCTATTGTGT+TGG + Chr5:6286060-6286079 None:intergenic 30.0%
TTGTAGTGTTCAACAAGTAT+AGG - Chr5:6286913-6286932 MsG0580024552.01.T01:intron 30.0%
! AAGTTTTGCTAAAAGCTTCT+TGG - Chr5:6286799-6286818 MsG0580024552.01.T01:intron 30.0%
! CTCTTTTGATAAAAACATGG+AGG - Chr5:6286069-6286088 MsG0580024552.01.T01:CDS 30.0%
!! TAGTTAGTTGATGACATGAA+TGG - Chr5:6286252-6286271 MsG0580024552.01.T01:intron 30.0%
!! TTTAGTTTGGCAAACTTCAA+AGG - Chr5:6286281-6286300 MsG0580024552.01.T01:intron 30.0%
AACAGTGAGCTCAAATTACT+TGG + Chr5:6286445-6286464 None:intergenic 35.0%
AACTTTCTTCTGTTGAACGT+TGG + Chr5:6286386-6286405 None:intergenic 35.0%
AAGTATAGGAACAGTTCCAT+TGG - Chr5:6286927-6286946 MsG0580024552.01.T01:intron 35.0%
ACAACGACGAAGAAAATTCT+TGG - Chr5:6286869-6286888 MsG0580024552.01.T01:intron 35.0%
ACAGTGAGCTCAAATTACTT+GGG + Chr5:6286444-6286463 None:intergenic 35.0%
ACTGTCTTCTTATACTTGGT+CGG + Chr5:6286601-6286620 None:intergenic 35.0%
AGGTAAATAAAGATGAAGGC+CGG - Chr5:6286005-6286024 MsG0580024552.01.T01:CDS 35.0%
CAAATATCACGGAGATTACA+GGG - Chr5:6287013-6287032 MsG0580024552.01.T01:intron 35.0%
CACTACAATAATGTCTTTCC+TGG + Chr5:6286901-6286920 None:intergenic 35.0%
CAGTTAAGTGATGACATGAA+TGG - Chr5:6286345-6286364 MsG0580024552.01.T01:intron 35.0%
CTAACTAATGACCTACTTCA+AGG + Chr5:6286239-6286258 None:intergenic 35.0%
GAAAATTCTTGGAAGAAACC+AGG - Chr5:6286880-6286899 MsG0580024552.01.T01:intron 35.0%
GCAAAACTTGTGCTAATATC+AGG + Chr5:6286788-6286807 None:intergenic 35.0%
GTGGAGGTAAATAAAGATGA+AGG - Chr5:6286001-6286020 MsG0580024552.01.T01:CDS 35.0%
GTTCACTGTCTTCTTATACT+TGG + Chr5:6286605-6286624 None:intergenic 35.0%
TCAAATATCACGGAGATTAC+AGG - Chr5:6287012-6287031 MsG0580024552.01.T01:intron 35.0%
TTGTGTTGGACACAAACTTT+CGG + Chr5:6286046-6286065 None:intergenic 35.0%
! AGAAGCATGCTTAACATCAT+TGG - Chr5:6286654-6286673 MsG0580024552.01.T01:intron 35.0%
! TATGGCTGCAAAATCTTTTC+CGG + Chr5:6286544-6286563 None:intergenic 35.0%
AAAACTGGTACAATAGCGTG+AGG - Chr5:6286728-6286747 MsG0580024552.01.T01:intron 40.0%
AAGACAGTGAACTGCAATCA+TGG - Chr5:6286613-6286632 MsG0580024552.01.T01:CDS 40.0%
AGTGCCTTATGTTGCATGTA+TGG + Chr5:6286562-6286581 None:intergenic 40.0%
CCATTGGCATGTGAAAAGAA+TGG - Chr5:6286943-6286962 MsG0580024552.01.T01:intron 40.0%
TAATATCAGGCTTCTCAGCT+TGG + Chr5:6286775-6286794 None:intergenic 40.0%
TGCAATGAGACCTACAAAAC+TGG - Chr5:6286690-6286709 MsG0580024552.01.T01:intron 40.0%
! AAGAGATGGGTTCATAAGGT+TGG - Chr5:6287099-6287118 MsG0580024552.01.T01:CDS 40.0%
! CCATTCTTTTCACATGCCAA+TGG + Chr5:6286946-6286965 None:intergenic 40.0%
! GCTTAAGAGATGGGTTCATA+AGG - Chr5:6287095-6287114 MsG0580024552.01.T01:CDS 40.0%
!! CGCTATTGTACCAGTTTTGT+AGG + Chr5:6286726-6286745 None:intergenic 40.0%
!! GAATGGTGTTGCAAAGAGAA+AGG - Chr5:6286960-6286979 MsG0580024552.01.T01:intron 40.0%
!! TCTCATTGCACCAGTTTTGT+AGG + Chr5:6286703-6286722 None:intergenic 40.0%
!! TTGAGCTCACTGTTTTTCTG+TGG - Chr5:6286451-6286470 MsG0580024552.01.T01:CDS 40.0%
!!! TGTTTTTCTGTGGTGGACAA+TGG - Chr5:6286461-6286480 MsG0580024552.01.T01:CDS 40.0%
AGAAACGTAAACCTGACCGT+AGG - Chr5:6287052-6287071 MsG0580024552.01.T01:CDS 45.0%
AGCAACCTAGGCTTAAGAGA+TGG - Chr5:6287085-6287104 MsG0580024552.01.T01:CDS 45.0%
CATGCTTCTCCATTCCAAAC+AGG + Chr5:6286643-6286662 None:intergenic 45.0%
CATGGAAGTCCTGTTTGGAA+TGG - Chr5:6286631-6286650 MsG0580024552.01.T01:intron 45.0%
GCAACCTAGGCTTAAGAGAT+GGG - Chr5:6287086-6287105 MsG0580024552.01.T01:CDS 45.0%
GCAATCATGGAAGTCCTGTT+TGG - Chr5:6286626-6286645 MsG0580024552.01.T01:intron 45.0%
GCAGCCATACATGCAACATA+AGG - Chr5:6286555-6286574 MsG0580024552.01.T01:CDS 45.0%
GCTCAATTGCACCTTGAAGT+AGG - Chr5:6286225-6286244 MsG0580024552.01.T01:intron 45.0%
GGTCAGGTTTACGTTTCTGT+GGG + Chr5:6287050-6287069 None:intergenic 45.0%
TCACGGAGATTACAGGGAAA+TGG - Chr5:6287019-6287038 MsG0580024552.01.T01:intron 45.0%
TGAACCCATCTCTTAAGCCT+AGG + Chr5:6287093-6287112 None:intergenic 45.0%
!! AGCTCACTGTTTTTCTGTGG+TGG - Chr5:6286454-6286473 MsG0580024552.01.T01:CDS 45.0%
AGCCTAGGTTGCTTCAAAGC+AGG + Chr5:6287078-6287097 None:intergenic 50.0%
CGGTCAGGTTTACGTTTCTG+TGG + Chr5:6287051-6287070 None:intergenic 50.0%
GTTGCTTCAAAGCAGGCCTA+CGG + Chr5:6287071-6287090 None:intergenic 50.0%
TCGGTTGCACATTCTCGAAC+CGG + Chr5:6286027-6286046 None:intergenic 50.0%
!! TTCTGTGGTGGACAATGGCA+GGG - Chr5:6286466-6286485 MsG0580024552.01.T01:CDS 50.0%
!! TTTCTGTGGTGGACAATGGC+AGG - Chr5:6286465-6286484 MsG0580024552.01.T01:CDS 50.0%
TTCAAAGCAGGCCTACGGTC+AGG + Chr5:6287066-6287085 None:intergenic 55.0%
!! GGCCTGCTTTGAAGCAACCT+AGG - Chr5:6287073-6287092 MsG0580024552.01.T01:CDS 55.0%
GGTGGACAATGGCAGGGCGA+TGG - Chr5:6286472-6286491 MsG0580024552.01.T01:CDS 65.0%
! TGGCAGGGCGATGGTAGTGC+TGG - Chr5:6286481-6286500 MsG0580024552.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr5 gene 6285989 6287137 6285989 ID=MsG0580024552.01;Name=MsG0580024552.01
Chr5 mRNA 6285989 6287137 6285989 ID=MsG0580024552.01.T01;Parent=MsG0580024552.01;Name=MsG0580024552.01.T01;_AED=0.49;_eAED=0.51;_QI=0|0|0|1|0.5|0.66|3|0|181
Chr5 exon 6287036 6287137 6287036 ID=MsG0580024552.01.T01:exon:2470;Parent=MsG0580024552.01.T01
Chr5 exon 6286415 6286639 6286415 ID=MsG0580024552.01.T01:exon:2469;Parent=MsG0580024552.01.T01
Chr5 exon 6285989 6286207 6285989 ID=MsG0580024552.01.T01:exon:2468;Parent=MsG0580024552.01.T01
Chr5 CDS 6287036 6287137 6287036 ID=MsG0580024552.01.T01:cds;Parent=MsG0580024552.01.T01
Chr5 CDS 6286415 6286639 6286415 ID=MsG0580024552.01.T01:cds;Parent=MsG0580024552.01.T01
Chr5 CDS 6285989 6286207 6285989 ID=MsG0580024552.01.T01:cds;Parent=MsG0580024552.01.T01
Gene Sequence

>MsG0580024552.01.T01

ATGAGGGAAACAGTGGAGGTAAATAAAGATGAAGGCCGGTTCGAGAATGTGCAACCGAAAGTTTGTGTCCAACACAATAGCTCTTTTGATAAAAACATGGAGGGCGATGGTAGTGCTGGAATGAAGAAAATTTTAACTATTGACAATACTTTAAATCCGGAAAAGATTTTGCAGCCATACATGCAACATAAGGCACTTCATCATACTCTTGATCCGACCAAGTATAAGAAGACAGTGAACTGCAATCATGGAAGTCCTGTTTGGAATGGAGAAGCATGCTTAACATCATTGGACACTGATACAAGTGCAATGAGACCTACAAAACTGTGTTCAACAAGTATAGGAACAGTTCCATTGGCATGTGAAAAGAATGGTGTTGCAAAGAGAAAGGCATTGACAGAAGCAACCAATTTTCAACAGTCAAATATCACGGAGATTACAGGGAAATGGCAATGCCCACAGAAACGTAAACCTGACCGTAGGCCTGCTTTGAAGCAACCTAGGCTTAAGAGATGGGTTCATAAGGTTGGAAAGATCTCTCAGTGA

Protein sequence

>MsG0580024552.01.T01

MRETVEVNKDEGRFENVQPKVCVQHNSSFDKNMEGDGSAGMKKILTIDNTLNPEKILQPYMQHKALHHTLDPTKYKKTVNCNHGSPVWNGEACLTSLDTDTSAMRPTKLCSTSIGTVPLACEKNGVAKRKALTEATNFQQSNITEITGKWQCPQKRKPDRRPALKQPRLKRWVHKVGKISQ*